WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2026
·Vol. 42
·期 5
·213
·
IF 4.7
Huan Tong,Zhang Jing-Yi,Huang Jia-Ying,Si Wen-Jie,Wu Yue,Zhang Ye-Wang
Chondroitinase ABC (ChSase ABC), which can digest chondroitin sulfate (CS), has been utilized for the analysis of CS glycan structure and the preparation of low-molecular-weight chondroitin sulfate. However, the catalytic activity of most ChSase ABCs remains insufficient for industrial or large-scale applications. Previous structure studies of ChSase ABCs indicate that the N-terminal domain is typically involved in substrate recognition and binding. Furthermore, the N-terminal domain of the ChSase ABC from Bacteroides thetaiotaomicron ( Bt ChSase ABC) also exhibits similarity with the carbohydrate-binding module of other glycosidases. In the present work, the N-terminal of Bt ChSase ABC was chosen for engineering. Through multiple sequence alignment, 9 non-conserved residues were selected as candidate mutation sites, and a mutant library containing 20 variants was constructed by single-point and combinatorial mutations. Among them, the catalytic activity of 13 mutants showed varying degrees of improvement. The mutant L137V showed 79.29% and 84.49% increases in specific activity and V max , respectively, compared to the wild type. SAX-HPLC analysis of enzymatic hydrolysates confirmed that the mutation retained the enzyme’s substrate recognition and cleavage mechanism. Kinetic parameters revealed that the enhanced catalytic activity of the mutants was mainly attributed to an increased turnover number ( k cat ), indicating that the rate-limiting step of catalysis was effectively accelerated.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2026
·
IF 9.2
Linyu Zhang,Mianyuan Wu,Yushui Han,Xuefeng Bai
Co/PDA-Al 2 O 3 precursors were prepared via a NaBH 4 reduction process with polydopamine-modified alumina (PDA-Al 2 O 3 ) as the carrier. Co 4 Pd/PDA-Al 2 O 3 catalysts were prepared via the galvanic replacement reaction (GRR) method with Co/PDA-Al 2 O 3 as the template and reducing agent. The above-prepared catalysts were analyzed for composition, structure, and morphology. The abundant N and O groups on the PDA-Al 2 O 3 surface anchored Co NPs, which promoted the uniform distribution of PdCo NPs with an average particle size of 2.92 nm. Ultrafine PdCo NPs and the coordination and strain effects between Pd and Co enhanced the catalytic performance of dodecahydro-N-ethylcarbazole (12H-NEC) dehydrogenation. The dehydrogenation efficiency of 12H-NEC catalyzed by Co 4 Pd/PDA-Al 2 O 3 achieved 100% at 180 °C for 4 h, and 98.45% at 170 °C for 6 h. The catalytic performance of 12H-NEC continuous dehydrogenation was evaluated in the fixed-bed reactor using Co 4 Pd/PDA-Al 2 O 3 as catalyst. The dehydrogenation efficiency was maintained at 95.4% for 100 h, indicating the excellent stability.
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MACROMOLECULES
·
2026
·
IF 5.7
Chi Li,Jiayu Yu,Wei Jiang,Jing jin,Xiangling Ji,Yanxiong Pan
Looped or cyclic polymers without chain ends exhibit unusual physicochemical properties and have been explored for emerging applications in surface coatings, nanolithography and electronic patterning, and drug or gene delivery. However, there is little information reported on the topological effect of the thermoresponsive looped brush on corresponding properties. Herein, two types of poly(N-isopropylacrylamide) (PNIPAM) terminated with one and two thiol groups are selected to bond with gold chips to form linear and looped PNIPAM brushes. The difference in conformational transition and protein adsorption behaviors for the two types of brushes at different temperatures has been evaluated using quartz crystal microbalance with dissipation measurements. The looped brushes exhibit anomalous hydration retention and negligible thermal hysteresis. Crucially, looped topology overcomes dehydration by enforcing extended conformations that can sustain the hydration barriers during collapse and further reduces protein adsorption. This work provides new insights into understanding the impact of different topological structures on conformational transitions and the corresponding protein adsorption, enriching the scope of research on temperature-responsive polymers.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Hao Fang,Yuan Li,Haotian Cheng,Shiyi Song,Fuping Lu,Yi-Heng P. Job Zhang
Lacto-N-biose I (LNB), a core structural unit of human milk oligosaccharides, was usually fermented by microbial cell factories because its biosynthesis involved the supplies of ATP and UDP-sugars. Here we designed an in vitro ATP- and UDP-sugar-free enzymatic pathway for the biosynthesis of LNB from lactose and N-acetylglucosamine (GlcNAc). Thermoclostridium caenicola cellobiose phosphorylase was discovered to have a very high promiscuous activity of lactose phosphorylase. Its lactose phosphorylase activity was enhanced greatly by directed evolution, yielding the variant M2 (N654A/Y501A) having 1.56-fold higher lactose activity and doubled lactose/cellobiose specificity. The coenzyme-free three-enzyme molecular machine containing lactose phosphorylase M2, lacto-N-biose phosphorylase and polyphosphate glucokinase produced up to 115 g/L LNB from lactose and GlcNAc and exhibited the highest volumetric productivity of 14.4 g/L/h. This coenzyme-free multienzyme molecular machine could provide a cost-competitive platform for in vitro biomanufacturing of LNB.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2026
·
IF 19.7
Longyu Qiu,Menggang Li,Fenyang Tian,Lin He,Wenxin Zhu,Fengyu Wu,Xue Ren,Tongbo Zhang,Zhekai Zhang,Shipeng Zhang,Jiecai Han,Weiwei Yang,Yongsheng Yu
Proposing an in-plane N-bridged structure for atomic-level robust coordination. • Strong orbital overlap redirects hydroxy adsorption from Ni/Fe sites to Ru atoms. • Optimized coordination alleviates scaling relations and suppresses Ni site oxidation. • AEMWE achieves 2 A cm 2 at 1.84 V with ultra-stable operation over 800 h. The pursuit of high-performance anion exchange membrane water electrolysis (AEMWE) catalysts is limited by conventional supported and lattice-doped materials, which suffer from weak interfacial coupling and suboptimal intermediate adsorption, leading to degradation under industrial conditions. Although atomic-level modulation is highly appealing, achieving a synergistic balance between robust structural stability and precise control over the reaction pathway remains challenging. Herein, by employing a nitrogen-coordinated metal bridging strategy, we report a Ru-NiFeN with atomically coordinated Ru-N bridges for practical water electrolysis. This configuration manipulates the local electronic microenvironment to trigger a site-specific redirection of OH adsorption from Fe to Ru centers, effectively bypassing host-lattice scaling-relation limitations and lowering OER barriers. In-situ spectroscopy and theoretical calculations reveal that the Ru-N bridge promotes resilient dynamic structural reconstruction, enabling potential-triggered NiOOH formation while suppressing detrimental metal oxidation. The Ru-NiFeN catalyst achieves a low overpotential of 340 mV at 1 A cm 2 and demonstrates outstanding AEMWE durability, operating for over 800 h at 2 A cm 2 with a decay rate of only 24.4 μV h 1 , positioning it among the most active catalysts reported. This work provides a strategic pathway for optimizing adsorption landscapes via atomic coordination engineering for efficient, stable energy conversion. Download: Download high-res image (191KB) Download: Download full-size image
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2026
·
IF 19.7
Yongxin Guo,Hongda Zhang,Siji Chen,Bolun Zhang,Jiaoyang Ji,Gang Wang,Sitong Zhang,Yong Yu
A novel In 2 S 3 /N-BC@PVA hydrogel evaporator was developed for water purification. • The In 2 S 3 /N-BC heterojunction facilitated interfacial charge redistribution and separation. • Synergistic effect achieves > 99% ARB and > 95% ARG removal. • The evaporator achieves a high evaporation rate (3.93 kg m −2 h −1 ). An In 2 S 3 /N-BC@PVA evaporator is constructed based on the synergistic effect of photothermal catalysis and the regulation mechanism of water molecules, which demonstrates the inactivation rate of ARB (99.77 ± 0.12%), removal rate of ARGs (95–99%), and evaporation rate (3.93 ± 0.16 kg·m −2 ·h −1 ). In 2 S 3 /N-BC reduces the equivalent evaporation enthalpy by activating intermediate water within the hydrogel network. At the In 2 S 3 /N-BC interface, strong interactions induce a metallic-like electronic structure characterized by a high density of states near the Fermi level and a reduced work function, which facilitate spatial charge separation and directed the migration of photogenerated carriers. The -NH 2 is electron-bridging sites to facilitate interfacial charge transfer, which promotes the generation of •OH via hole oxidation. Outdoor testing confirms stable freshwater yield and antibacterial efficacy in 30 d. This work integrates photocatalysis and interfacial evaporation in water remediation strategy, and provides a sustainable approach to address antibiotic resistance and freshwater scarcity. Download: Download high-res image (289KB) Download: Download full-size image
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PEST MANAGEMENT SCIENCE
·
2026
·
IF 4.2
Wang Geng,Ruirui Zhao,Wei Zhang,Jinsong Yang,Kai Yan,Xiuhai Gan
BACKGROUND Protoporphyrinogen IX oxidase (PPO, EC 1.3.3.4) is an important target for the development of highly efficient and environmentally friendly herbicides. Unfortunately, conventional N -phenylphthalimide-derived PPO inhibitors are often plagued by limited herbicidal spectra and unsatisfactory crop selectivity. Therefore, a series of novel and highly active broad-spectrum N -phenylphthalimide herbicidal inhibitors were designed and synthesized via the introduction of amide and isoindolinone moieties. RESULTS Based on the active substructure splicing strategy, a series of N -phenylphthalimide derivatives were synthesized. Among them, compound C4 showed a strong inhibitory effect on Nicotiana tabacum PPO ( Nt PPO) with an inhibition constant ( K i = 38.1 n m ), which was superior to Flumiclorac-pentyl ( K i = 46.3 nM) and Flumioxazin ( K i = 52.0 n m ). Glasshouse assays indicated that compound C4 showed excellent herbicidal activity against six weed species at 150 g a.i. ha −1 and exhibited higher safety on seven crops. Target validation demonstrated that compound C4 could completely inhibit the growth of the ΔhemG- Escherichia coli strain complemented with Arabidopsis thaliana PPO (∆hemG- At PPO) at 100 μ m . Computer simulation results showed that compound C4 could not only form strong hydrogen-bonds with Arg98 (2.9 Å) and alkyl–π interactions with the Gly65, Phe439, and Trp435 residues of Nt PPO, but also possessed excellent chemical reactivity, good electrostatic field characteristics, and high polarity. CONCLUSION Studies have shown that the N -phenylphthalimide derivative C4 , containing amide and isoindolinone structural moieties, can serve as a potential PPO inhibitor. © 2026 Society of Chemical Industry.
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AICHE JOURNAL
·
2026
·
IF 4.4
Zhou Chen,Shengyao Lv,Zhongyao Zhang,Wenyao Chen,Gang Qian,Jing Zhang,Xinggui Zhou,De Chen,Weikang Yuan,Xuezhi Duan
Alkylation of amines with alcohols offers a sustainable route featuring high atom economy and minimal waste, yet remains kinetically challenging due to the inertness of alcohols and the need for coordinated activation of multiple sites. In this work, we report a strategy of engineering Ni/NiAl m O heterojunction structure that synergizes Ni 0 and Ni(Al)O sites toward efficient N-alkylation. A series of Ni/NiAl m O catalysts with varied Ni/Al ratios were prepared by coprecipitation and characterized. The results show a gradual evolution of the oxide phase from Ni(Al)O toward NiO with decreasing Al content, with Ni/NiAl 0.5 O exhibiting the most apparent Ni/Ni(Al)O heterojunction and the highest catalytic activity. Density functional theory calculations demonstrate that Ni(Al)O lowers the barrier for O H cleavage in ethanol relative to NiO, thereby cooperating with Ni 0 sites for subsequent C H activation. This multisite synergistic strategy provides a new avenue for the rational design and preparation of efficient N-alkylation catalysts.
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RSC Advances
·
2026
·Vol. 16
·期 28
·25717-25731
·
IF 6.1
Zhao Du,Yanan Wu,Mengmeng Yu,Zishuang Cheng,Po Hu,Zhonglu Guo,Guifeng Chen,Chengchun Tang,Yi Fang
Herein, porous boron nitride nanosheets (BNNS) exhibiting n-type semiconductor characteristics were synthesized via high-temperature pyrolysis. Subsequently, a series of Bi2O3/BNNS composites with superior photocatalytic activity were constructed using a solvothermal method. Experimental results demonstrate that the optimized composite photocatalyst exhibits significantly enhanced adsorption capacity and photocatalytic activity compared to pristine BNNS and Bi2O3. The optimal Bi2O3/BNNS composite achieved degradation efficiencies of 94.65%, 94.23%, and 93.97% for tetracycline (TC), oxytetracycline (OTC), and doxycycline (DC) (each at 50 mg L−1), respectively, under simulated solar irradiation. This study highlights that the exceptional adsorption capability of BNNS enables the Bi2O3/BNNS composite to fulfill the requirements for synergistic adsorption–photocatalysis. Furthermore, BNNS serves as an effective growth substrate, significantly regulating the growth of Bi2O3 nanowires and suppressing their agglomeration, thereby endowing the composite with a large specific surface area and pore volume. The formation of a p–n heterojunction also effectively suppresses the recombination of photogenerated charge carriers within the catalyst. Finally, this work elucidates the detailed process and underlying mechanism of photocatalytic tetracycline degradation driven by simulated sunlight. In summary, this study innovatively employs the Bi2O3/BNNS composite as a novel photocatalyst for tetracycline degradation and provides theoretical guidance for the design of advanced photocatalysts.
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ACS Applied Polymer Materials
·
2026
·
IF 5.2
Liu Yang,Hu Guangkai,Zhang Xin,Lei Cong,Li Yongxiang
Highly flexible carbon-based electrochemical sensing
electrodes
remain a challenge. Introducing conducting polymers into carbon-based
materials is an effective strategy to enhance mechanical flexibility.
In this work, an n-type conducting polymer, poly(benzodifurandione)
(PBFDO), with intrinsic flexibility and ultrahigh conductivity (2000
S cm –1 ) was introduced into carbon-based ink (named
C:PBFDO ink). PBFDO covers the cracks in a carbon-based film, enabling
a uniform surface to dissipate stress. The electrical conductivity
of the C:PBFDO composite film (6.0 ± 0.5 S cm –1 ) was twice that of the carbon-based film, supporting a half-thickness
electrochemical sensing electrode design and further improving the
mechanical flexibility. After 10,000 bending cycles (a bending radius
of 1 mm), the sheet resistance of the C:PBFDO electrode increased
by less than 15%, and the electrochemical performance did not decline
significantly compared with that of the severely attenuated carbon-based
electrodes (CEs). The C:PBFDO composite electrode demonstrates excellent
mechanical flexibility, electrochemical performance, and storage stability,
making it a promising candidate for applications in flexible wearable
electronics.
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Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 5
·124738
·
IF 7.5
Xinyi Tao,Weiwei Chen,Xuehong Fan,Rui Hu,Xuejian Wang,Shiquan Sun,Yu-Hong Cui,Zheng-Qian Liu
Waste-derived biochar catalysts are widely used in catalytic ozonation. However, biochar is often constrained by insufficient surface active sites and low interfacial electron transfer efficiency. In this work, nitrogen (N) and sulfur (S) co-doped sediment-derived biochar (C-N 1 S 2 -600) was prepared by one-step pyrolysis using a mixed compost of black-odorous sediment, reed straw, and food waste as precursors, with melamine and thiourea using as the N and S sources, respectively. The optimized C-N 1 S 2 -600 has a well-developed mesoporous structure, abundant structural defects, and numerous reactive sites originating from N/S–C coordination. Ozonation with C-N 1 S 2 -600 achieved 62.8% Deethylatrazine degradation, which was significantly higher than that of ozonation alone (13.9%) and ozonation with pristine biochar (33.1%). C-N 1 S 2 -600 also exhibited excellent reusability over five consecutive cycles and maintained high performance in secondary effluent. Reactive species identification confirmed that C-N 1 S 2 -600 facilitated the conversion of O 3 into • OH. Theoretical calculations further revealed that N, S co-doping induced a more non-uniform surface charge distribution and narrowed the orbital energy gap, providing electronic-level insights into enhanced O 3 adsorption, interfacial electron transfer, and reactive oxygen species generation. C-N 1 S 2 -600 exhibited good operational stability and tolerance to matrix interference under the tested conditions. This study provides a feasible strategy for converting solid-waste-derived biochar into efficient carbonaceous catalysts for advanced wastewater treatment, and offers a promising strategy for micropollutant abatement and wastewater purification by catalytic ozonation with high-performance biochar catalyst.
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APPLIED CATALYSIS A-GENERAL
·
2026
·
IF 4.8
Huimin Luo,Jinming Yan,Deyun Xu,Jia Guo,Mengjie Fan,Longtian Kang,Qingfu Sun
P/N-type Z-scheme heterojunction of Co-PDI/Cu-CuTCPP is prepared by a simple way. • Multifunctional photocatalytic activity of is achieved for POE, PHE and CO 2 RR. • Catalytic activities for POE and CO 2 RR increase by ~3 and ~12 times, respectively. • The overall CO 2 RR in the aqueous phase can be achieved to yield O 2 , CO and CH 4 . • It is found that Co-O-Cu linkage exists and has important role in photocatalysis. Developing a multifunctional system for photocatalytic water splitting (PWS) and carbon dioxide reduction reaction (CO 2 RR) is a promising approach to achieve the green carbon cycling. Here, the p/n-type Z-scheme heterojunction (Co-PDI/Cu-CuTCPP) between cobalt perylene diimide nanorods (Co-PDI) and copper porphyrin metal-organic framework nanosheets (Cu-CuTCPP NSs) is constructed via a cetyltrimethylammonium bromide (CTAB)-assisted physical method. Systematical characterizations prove the existence of Co–O–Cu linkage, π-π interaction, and electrostatic forces between Co-PDI and Cu-CuTCPP. The effect of Co-PDI/Cu-CuTCPP ratio on the optical, photoelectric and electrical properties, as well as multifunctional photocatalytic activity is investigated and analyzed. Under visible light irradiation (λ > 420 nm), the Co-PDI/Cu-CuTCPP composites exhibit the photocatalytic activities of water splitting to H 2 and O 2 , as well as CO 2 RR to CO and CH 4 , respectively. After optimizing photocatalytic system, the oxygen evolution rate of 18.7 mmol·g −1 ·h −1 is reached, which is a 4.4-fold compared that of pure Co-PDI. The photogenerated electron conversion efficiency during CO 2 RR is 12.9-fold higher in Co-PDI/Cu-CuTCPP than in Cu-CuTCPP. Meanwhile, the overall CO 2 RR in the aqueous phase can be achieved without H 2 production. Mechanism studies reveal the photoelectrical conversion process, and confirm the synergistic role between Co-PDI and Cu-CuTCPP. Download: Download high-res image (310KB) Download: Download full-size image
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