Food Analytical Methods
·
2022
·Vol. 16
·期 2
·318-329
·
IF 3.498
Yun Ning,Lu Chaoqun,Sun Tian,Xu Baocai,Song Yushen,Zong Zibing,Chen Kangwen,Huang Ganhui,Chen Xingguang,Gu Qianhui
Red pepper oil is a kind of condiment used in food factories, and its spicy degree can be reflected by the content of capsaicin. However, the classification method of red pepper oil is too subjective to be applied in food factories. In our work, a sensitivity electrochemical sensor for detecting capsaicin was constructed based on the glassy carbon electrode (GCE) modified with β-cyclodextrin (β-CD) assisted reduced graphene oxide (rGO). The results showed that the introduction of β-CD made rGO more dispersed, increasing the electrochemical active surface area of β-CD/rGO, thus improving the charge transfer rate. Furthermore, polycyclodextrin with selective recognition ability was dispersed on the surface of rGO, providing the possibility of capsaicin enrichment on the surface of the sensor. Based on these reasons, the sensor had a lower detection limit (0.05 μg/mL), and acceptable stability and anti-interference. Most importantly, β-CD/rGO/GCE displayed a satisfactory recovery rate (94.83 ~ 115.75%) in the detection of red pepper oil, and there was no statistical significance difference between this method and the LC–MS method.
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Frontiers in Immunology
·
2022
·
IF 8.786
Tian Xiaoying,Fan Rongbo,He Hong,Cui Qingyu,Liang Xi,Liu Qiqi,Liu Tongjie,Lin Kai,Zhang Zhe,Yi Huaxi,Gong Piming,Zhang Lanwei
Food allergy is a serious public health problem because of its high incidence and risk. Probiotics can induce immune regulation in patients with allergic diseases, but its mechanism is not fully clear. In this paper, β-lactoglobulin (β-LG)-sensitized mice were used as models to explore the mechanism of Bifidobacterium animalis KV9 (KV9) and Lactobacillus vaginalis FN3 (FN3) on reducing allergic reactions and regulating immune cell function. The results showed that oral administration of KV9 and FN3 significantly reduced the scores of allergic symptoms, hypothermia symptoms, and serum levels of β-LG-specific immunoglobulins E (β-LG-sIgE), histamine, and mast cell protease in allergic mice. Flow cytometry analysis of intestinal dendritic cells (DCs) showed that the proportion of CD11c+major histocompatibility complex (MHC)-II+DCs, CD11c+CD80+DCs, and CD11c+ CD86+DCs increased after KV9 and FN3 intervention, indicating that the strains induced immature DCs and decreased the antigen-presenting capacity of DCs. Meanwhile, the toll-like receptor 4 (TLR4)-NF-κB signaling pathway was activated in DCs. The secretion of interleukin-12 (IL-12) was significantly increased, while interleukin-4 (IL-4) was decreased by DCs after KV9 and FN3 intervention, indicating that DCs have the potential to promote T-cell differentiation into T helper type 1 (Th1) cells. Furthermore, the proportion of CD3+CD8−IFN-γ+ T cells in the spleen increased, while CD3+CD8−IL-4+T cells decreased after oral administration of KV9 and FN3, correcting the T helper type 2 (Th2)-skewed immune responses. These results indicate that KV9 and FN3 reduce β-LG-induced allergic symptoms in mice, and suggest that the two potential probiotics might be used as an alternative therapeutic agent for mitigating food allergy.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Yuying Xu,Minting Liang,Jintao Huang,Yapei Fan,Haiyue Long,Qunjie Chen,Zhe Ren,Chaoxi Wu,Yifei Wang
Single helical β -glucan is a one-dimensional host that can form a hybrid helix with DNAs/RNAs as delivery systems. However, unmodified β -glucan has a gelling tendency and a single helical conformation is challenging to obtain. Therefore, in this study, we developed a β -glucan formyl derivative with stable single helical conformation and no gelling tendency. Circular dichroism studies found that the formyl- β -glucan could form a hybrid helix with DNA CpG-poly(dA). The hybrid helix delivery system showed improved activation on antigen-presenting cells, thereby upregulating the mRNA and protein levels of inflammatory factors, and had an immune-enhancing effect on ovalbumin (OVA) immunized mice. These results indicate that formyl- β -glucan can be developed as a non-cationic supramolecular DNA delivery platform with low toxicity and high efficiency.
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Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 46
·9639-9653
·
IF 7.571
Xinyuan Yuan,Teliang Lu,Fupo He,Tingting Wu,Xiaolan Wang,Jiandong Ye
Biomaterials in combination with multiple bioactive ions could create a favorable microenvironment for bone remolding. Herein, zinc silicate/β-tricalcium phosphate (ZS/β-TCP) composite ceramic scaffolds with different amounts of ZS (5, 10, and 15 wt%) were constructed using a three-dimensional fiber deposition (3DF) technique. The physicochemical, osteogenic and angiogenic properties of these interconnected macroporous scaffolds were investigated systematically. Simultaneously, GeneChip, alkaline phosphatase (ALP), western blot (WB) and polymerase chain reaction (PCR) were utilized to elucidate the underlying mechanism of the enhancement in osteogenic differentiation. The results showed that the incorporation of ZS significantly improved the mechanical performance by more than 5 fold in comparison with the β-TCP ceramic scaffold (4.79 ± 0.99 MPa). The ZS modified β-TCP scaffolds greatly supported the cytoactivity, adhesion, proliferation of mouse bone marrow mesenchymal stem cells (mBMSCs) and human umbilical vein endothelial cells (HUVECs). The expression levels of osteogenic genes and proteins as well as angiogenic genes were markedly upregulated by the sustained release of bioactive ions (mainly Si and Zn) from the composite scaffolds. The 10ZS/β-TCP demonstrated the best overall performance in vitro. Moreover, the 10ZS/β-TCP displayed a high bone volume fraction, bone maturity and angiogenesis after implantation in the rat skull defects for 6 weeks. It was further verified that ZS/β-TCP scaffolds stimulated the osteogenic differentiation of mBMSCs by activating the p38 signaling pathway directly. The 10ZS/β-TCP ceramic scaffold holds great potential for the fast repair of bone defects, and deep understanding of the mechanism will facilitate the formulation of new strategies for bone repair.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Xinyi Xing,Xian Shi,Rui Hu,Ying Guan,Hui Gao,Siquan Xu
In this work, a series of (x)Hf-β zeolite catalysts were prepared by a simple wet impregnation method and used as bifunctional catalysts with Lewis and Brønsted acid sites for synthesizing 5-hydroxymethylfurfural (5-HMF) from cellulose in H 2 O (NaCl) -THF biphasic system. Characterization analysis of (x)Hf-β zeolite catalysts with ICP-OES, N 2 adsorption-desorption isotherm, XRD, FTIR , SEM, NH 3 -TPD and Py-FTIR indicated that the catalysts possessed favorable pore structure, appropriate acid strength and acidity. A superior 86.3 % cellulose conversion and 53.4 % 5-HMF yield could be obtained at 200 °C for 4 h by using (5 %)Hf-β zeolite as the catalyst. In addition, the catalytic system provided a favorable 5-HMF yield from other carbohydrates, including glucose (85.1 %), sucrose (80.6 %), starch (65.1 %), maltose (65.0 %), dextran (59.9 %), and Moso bamboo (21.9 %). Nevertheless, the recyclability of (5 %)Hf-β zeolite catalyst was unsatisfactory and the 5-HMF yield showed a downtrend from 53.4 % to 24.4 % after 5 cycles. The catalyst has promising potential for converting biomass materials into high-value platform compounds.
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ANALYST
·
2022
·Vol. 147
·期 16
·3652-3661
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IF 5.227
Qinghao Cao,Chao Chen,Jun Huang,Dantong Shen,Haolin Chen,Huiqing Zhong,Zhiming Liu,Zhouyi Guo
The flexible surface-enhanced Raman scattering (SERS) platform has ceaselessly propelled the development of point-of-care testing (POCT) in diverse fields. Herein, we report a facile strategy for the SERS-chemometric analysis of four β-blockers (bisoprolol, metoprolol, acebutolol and esmolol) based on a super-sticky mussel-inspired hydrogel SERS tape. The surface morphology and mechanical properties of the hydrogel tape can be easily controlled by adjusting the compositional ratio. The optimized tape with excellent toughness and adhesiveness allows efficient collection of analytes through a simple “paste and peel off” approach, further by spraying with silver nanoparticles using a household sprayer to instantly assemble a flexible SERS substrate, the analytes can then detected by a portable Raman spectrometer. This POCT strategy enables the identification and discrimination of four similar β-blockers with high sensitivity and accuracy in combination with the statistical algorithms. The developed SERS tape is finally utilized for the recognition of β-blockers in simulated urine solution, which realizes a limit of detection of 1.0 ng mL−1, revealing a promising potential of this SERS-based POCT for the clinical detection of doping abuse.
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INORGANICA CHIMICA ACTA
·
2022
·
IF 3.118
Chang-Qing Miao,Ni Wang,Yan-Ni Ling,Xia-Qing Ma,Yu-Xin Chen,Rui-Feng Wang,Li-Hua Hou,Yu-Peng Hua,Min-Yan Kang,Ming Fang
Four new Ln III 2 compounds [Ln 2 (dbm) 2 (L) 2 (CH 3 OH)] (Ln(III) = Gd ( 1 ), Tb ( 2 ), Ho ( 3 ), and Tm ( 4 )), where H 2 L is 2-hydroxyamino-propionic acid (3,5-di- tert -butyl-2-hydroxy-benzylidene)-hydrazide and Hdbm is dibenzoylmethane, were successfully synthesized via solvothermal method and characterized structurally and magnetically. Magnetic studies reveal the anti-ferromagnetic interaction was existed in compound 1 . Moreover, magnetic refrigeration property study suggests that compound 1 exhibits magnetocaloric effect with − Δ S m = 21.07 J kg −1 K −1 for Δ H = 7.0 T at 2.0 K. Alternating-current (ac) magnetic susceptibility study of 2 and 3 suggests that single-molecule magnet (SMM) behavior occurs for 2 and 3 under H dc = 0 Oe with the effective energy barrier Δ E / k B of 20.05 K and τ 0 of 2.93 × 10 −6 s for 2 and Δ E / k B = 0.85 K and τ 0 = 3.47 × 10 −5 s for 3 .
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Catalysis Science & Technology
·
2022
·Vol. 12
·期 20
·6163-6173
·
IF 6.177
Jin Zhang,Mengting Gao,Ruiyi Wang,Xincheng Li,Pengqi Zhu,Yunwei Wang,Zhanfeng Zheng
Selective hydrogenation of α,β-unsaturated aldehydes under mild conditions is a great challenge in the synthesis of the corresponding alcohols. Herein, we report that a series of oxygen vacancy enriched MgAl-LDH coordinated transition metal hydroxide M(OH)x (M = Ru, Pt, Pd, Ag, Rh, Fe, Co, Ni and Cu) photocatalysts were successfully prepared taking advantage of the “memory effect” of MgAl-LDH. A high yield of α,β-unsaturated alcohols was achieved under visible light irradiation. DFT simulations and DRIFTS results show that oxygen vacancies facilitate the adsorption of –CO groups and hinder the adsorption of –CC groups in cinnamaldehyde, which ensures the high selectivity to cinnamyl alcohol. The highly dispersed and electron-rich transition metal hydroxides facilitated the activation of isopropanol to provide active H species under light irradiation and then enhanced the photocatalytic performance. This study demonstrated oxygen vacancy enriched MgAl-LDH supported transition metal hydroxide M(OH)x photocatalysts for efficient selective hydrogenation under visible light irradiation.
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EUROPEAN POLYMER JOURNAL
·
2022
·
IF 5.546
Pu Li,Changqing Tu,Miao-Miao Xun,Wan-Xia Wu
Vinyl-functionalized poly( β -thioether ester) (PVTE) was synthesized by enzyme-catalyzed polymerization using immobilized lipase B from Candida antarctica (CALB) for the first time. Four different functionalized monomers were prepared and three polymerization strategies including polycondensation (PC), ring-opening polymerization (ROP), and ring-opening and condensation copolymerization (ROCCP) were investigated. The influences of reaction conditions such as polymerization temperature, time, and monomer ratio on the molecular weight of functionalized poly( β -thioether ester)s were also studied. Both the homopolymer and copolymers were characterized by NMR, SEC, TGA and DSC. Moreover, these poly( β -thioether ester)s containing pendant double bonds toward postfunctionalization were demonstrated by photoinitiated thiol-ene click chemistry and Michael addition reaction, providing a way to cross-linked polyester gels.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Zunbin Duan,He Bian,Lijun Zhu,Daohong Xia
Efficient removal of thiophenic sulfides is important for the purification of fuel. Herein, 2-hydroxypropyl- β -cyclodextrin polymers (2-HP- β -CDPs) with micro-mesopores are synthesized using 2-HP- β -CD and tetrafluoroterephthalonitrile (TFTPN) for removing thiophenic sulfides. The cross-linking of 2-HP- β -CD and TFTPN units forms the mesopores, and the cyclodextrin cavity acts as the micropores. The 2-HP- β -CDP 1:4 (1:4 is the molar ratio of 2-HP- β -CD and TFTPN in preparation) possesses a surface area of 197.49 m 2 g –1 , and most of pores are at 1.09 nm of micropores and 6.06 nm of mesopores. The synergistic effect of the micro-mesopores promotes 2-HP- β -CDP 1:4 to remove the thiophenic sulfides in the order of benzo[ b ]thiophene > 4,6-dimethyldibenzothiophene ≈ dibenzothiophene > thiophene. The removal efficiency and rate using 2-HP- β -CDP 1:4 are better than those of CD-based and most traditional adsorbents that have been reported. Most of sulfides adsorbed by mesopores diffuse rapidly to micropores from the wide end of cyclodextrin and then are selectively captured by the cyclodextrin units in 2-HP- β -CDP 1:4 . These findings can guide the synthesis of CD-based polymers and the application of fuel purification.
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Foods
·
2022
·Vol. 11
·期 10
·1443
·
IF 5.561
Hongbo Li,Suya Xie,Shangqiao Cao,Liangbin Hu,Dan Xu,Jiayi Zhang,Haizhen Mo,Zhenbin Liu
Edible fungi play an important role in material and energy cycling. This study explored the role ofPleurotus eryngiimycelia in the transformation of potato high-calorie starch to low-calorie β-glucan. First, the 3D printing performance of the potato medium was optimized. After inoculating the fermentation broth ofPleurotus eryngiiin 3D printing, we studied the microstructure and material composition of the product. Along with the increase in 3D printing filling ratio, the starch content of the culture product decreased from 84.18% to 60.35%, while the starch content in the solid medium prepared using the mold was 67.74%. The change in β-glucan content in cultured products was opposite to that of starch, and the content of the culture product increased from 12.57% to 24.31%, while the β-glucan content in the solid medium prepared using the mold was 22.17%. The amino acid composition and content of the 3D printing culture system and solid culture products prepared using the mold were similar. The 3D printing culture system promoted the bioconversion efficiency of mycelia. It also showed high application potential ofPleurotus eryngiimycelia for the preparation of low-calorie food.Keywords:bioconversion;Pleurotus eryngiimycelia;starch;3D printing;β-glucan
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BONE
·
2022
·
IF 4.626
Senxin Cai,Yuchun Zou,Yong Zhao,Hanyu Lin,Dali Zheng,Linyu Xu,Youguang Lu
Aims Mechanical stress overload in the temporomandibular joint (TMJ) is an important cause of TMJ osteoarthritis (TMJOA). Whether secreted frizzled-related proteins (SFRPs) play important roles in the development of mechanical stress-induced TMJOA remains controversial. In this study, we investigated the roles of the Wnt/β-catenin signaling and SFRPs in the progression of mechanical stress-induced TMJOA. Methods We investigated the progression of mechanical stress-induced TMJOA using an in vivo model via modified increased occlusal vertical dimension (iOVD) malocclusion and an in vitro model in which isolated chondrocytes were subjected to mechanical stress. The effects of inhibition of Wnt/β-catenin signal on TMJOA induced by mechanical stress were studied by in vitro drug added and in vivo intra-articular injection of XAV-939. TMJOA progression, Wnt/β-catenin signaling and SFRPs was assessed by Cone beam computed tomography (CBCT) analysis, histochemical and immunohistochemical (IHC) staining, quantitative real-time PCR (qRT–PCR), Western blotting (WB), and immunofluorescence (IF) staining. Results Our in vivo results showed that iOVD-induced mechanical stress in the TMJ disrupted mandible growth, induced OA-like changes in TMJ cartilage, and increased OA-related cytokine expression. In addition, iOVD activated Wnt/β-catenin signaling and suppressed Sfrp1 , Sfrp3 , and Sfrp4 expression in condylar cartilage. Moreover, our in vitro study showed that stress disrupted homeostasis , activated Wnt/β-catenin signaling and inhibited SFRP3 and SFRP4 expression in chondrocytes. Suppression of Wnt/β-catenin signaling with XAV-939 promoted SFRP3 and SFRP4 expression and rescued mechanical stress-induced cartilage degeneration in vivo and in vitro. Conclusions Our work suggests that mechanical stress reduces SFRPs expression both in vivo and in vitro and promotes TMJOA via Wnt/β-catenin signaling. Suppression of Wnt/β-catenin signaling promotes SFRPs expression, especially SFRP3 and SFRP4 expression, and rescues mechanical stress-induced cartilage degeneration. Wnt/β-catenin signaling and SFRPs may represent potential therapeutic targets for TMJOA.
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