ANALYTICAL CHEMISTRY
·
2015
·
IF 5.636
Yingzi Liu,Meina Li,Fan Zhang,Anwei Zhu,Guoyue Shi
A Au disk nanoelectrode down to 3 nm in radius was developed by a facile and reliable method and successfully applied for monitoring dopamine release from single living vesicles. A fine etched Au wire was coated with cathodic electrophoretic paint followed by polyimide, which retracted from the tip end during curing to expose the Au nanotip. By cyclic voltammetric scanning the above tip in 0.5 M KCl, the transformation of a core-shaped apex into a geometrically well-defined Au disk nanoelectrode with different dimensions can be controllably and reproducibly achieved. Scanning electron microscopy, transmission electron microscopy, and steady-state voltammetry were used to determine the size of nanoelectrodes. The results showed that the specific etching and insulation method not only avoids the use of toxic etching solution and the uncontrollable treatment to expose the tip but also makes possible the controllable and reproducible fabrication of Au disk nanoelectrode down to 3 nm in radius. The nanoelectrodes with well-demonstrated analytical performance were further applied for amperometrically monitoring dopamine release from single rat pheochromacytoma cells with high spatial resolution.
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高影响因子
ONCOLOGY REPORTS
·
2015
·Vol. 34
·期 6
·3147-3155
·
IF 2.301
Lili Zhang,Jie Zhang,Li Chen,Jianli Wang
Autophagy is an intracellular multi-step catabolic degradation process that involves the degradation and recycling of cellular proteins and cytoplasmic damaged organelles to maintain cellular homeostasis and reduction of metabolic stress. Numerous studies have indicated the importance of autophagy in cancer, but the role of autophagy in human skin squamous cell carcinoma (SSCC) development and response to therapy it is still unclear. In the present study, we investigated the role of autophagy in SSCC and the relationship with chemotherapy sensitivity. The present study demonstrated that autophagy related gene the microtubule-associated protein 1 light chain 3 (LC3) expression was low in SSCC. The negative correlation with Bcl2 and survivin, and the chemotherapy drug 5-FU increased the level of autophagy and the autophagy inhibitor 3-MA inhibited this effect in SSCC cells, time- and dose-dependently. When SSCC cells were treated first with 3-MA and then with 5-FU, the inhibition of proliferation, migration, invasion and apoptosis of SSCC cells was enhanced. Our results suggested the possibility of autophagy as a potential target in SSCC therapy and 3-MA and 5-FU combination treatment may be an effective SSCC therapy via autophagy modulating.
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高影响因子
Journal of Materials Chemistry C
·
2024
·
IF 5.7
Yanyang Li,Huimin Du,Yue Ma,Meifang Liu,Jian Zou,Shentang Wang,Jun Yang,Shanshan Hu,Jun Lin
The existence of energy transfer channels from the self-trapped exciton (STE) emission to lanthanide (Ln3+) ions in double perovskites (DPs) opens up new possibilities for high security anti-counterfeiting applications. In the present investigation, we synthesized Sb3+/Ho3+/Er3+ doped Cs2NaGdCl6 rare-earth-based DPs using a simple solvothermal method, whose luminescence could be quenched by water and restored after drying. The efficient down-shifting (DS) red emission from the 4f–4f transition of Ho3+ was enhanced 8-fold due to the energy transfer of STEs → Ho3+ ions via Sb3+ sensitization. The DS and up-conversion (UC) dual-mode multicolor emissions were obtained by further introducing Er3+. Specifically, the DS luminescence of the tri-doped sample under the UV region and that under 448 nm irradiation were white and red, respectively; and the UC luminescence under 980 nm irradiation was green. In view of their stimulus response to water and dual-mode multicolor luminescence properties, advanced anti-counterfeiting materials based on the obtained samples were designed.
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高影响因子
Nanomaterials
·
2017
·Vol. 7
·期 2
·25
·
IF 3.553
Xiang-Nan Xu,Xiao-Na Guan,Hui-Hua Zhou,Yue-Feng Zhu
3-Hydroxy-2-naphthoic acid hydrazide (HNH), a new reductant and modifier, was applied to reduce and modify graphene oxide (GO) in a one-step process. The obtained HNH reduced graphene oxide (HNH-rGO) was characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), Raman spectroscopy, X-ray photoelectron spectroscopic (XPS) and Fourier transform infrared spectra (FTIR). The results demonstrated that GO was successfully reduced to graphene and the surface of HNH-rGO was grafted with HNH. The interlayer space was increased from 0.751 nm to 1.921 nm, and its agglomeration was much more attenuated compared with GO. HNH-rGO/polypropylene and graphene/polypropylene composites were synthesized through melt-blending method. The viscosity was enhanced with increased addition of graphene and surface modified graphene demonstrated stronger rheological behavior improving effect than the untreated graphene.Keywords:graphene;reduction;surface modification;nanocomposites
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Fengping Liu,Jiaqian Tang,Jun Xu,Yun Shu,Qin Xu,Hongmei Wang,Xiaoya Hu
An amperometric sensor was firstly established for the detection of indole-3-acetic acid (IAA) at low potential based on the hemin/reduced graphene oxide (hemin/rGO) composite. The hemin/rGO nanocomposite was prepared by a simple and facile hydrothermal method without using any reducing agent. It exhibited peroxidase-like activity for the catalytic oxidation of IAA in the presence of oxygen. The consumption of oxygen has a linear relationship with the concentration of IAA in the range from 0.1 to 43 μM and from 43 to 183 μM. The detection limit was down to 0.074 μM. This sensor was unaffected by many interfering substances and stable over time. Such work broadened the application of hemin/rGO and provided a new method for IAA detection.
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高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2017
·Vol. 21
·期 6
·1631-1637
·
IF 2.316
Ma Xinying,Wang Xueliang,Chen Meifeng,Li Fuchuan
A novel electrochemical immunoassay for glypicans-3 (GPC3) was established based on its new high affinity antibody (Ig). In this study, we used glass carbon film (GCF) as carrier for immobilizing Ig and avoiding the high electron transfer resistance (Ret) in electrochemical immunoassay sensor (Ig-Biotin-GR/GCE) because many materials such as bovine serum albumin (BSA), avidin, biotin, Ig, GPC3, and ALP were covered on. The Ig was combined with GCP3 labeled alkaline phosphatase (ALP-GPC3) which catalyzes p-nitro phenyl phosphate (PNPP) into p-nitro phenol (PNP). In order to obtain highly sensitive electrochemical signals of the PNP and further determine ALP-GPC3, the GR/GCE modified polylysine (PLYS/GR/GCE) was fabricated and used for detecting the PNP. According to the principle of competitive inhibition, less ALP-GPC3 would be combined with the Ig in the presence of GPC3. By investigating the signals of PNP in the absence and presence of GPC3, GPC3 could be qualitatively and quantitatively analyzed. Under the optimal conditions, the peak current of PNP on the PLYS/GR/GCE was linear with the concentration of GPC3 in the range of 0.75~75 ng mL −1 and a detection limit of 0.2 ng mL −1 . The recognition of GPC3 and detection of PNP were separated, which provides a new way for studying the diagnosis of liver cancer.
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PLoS One
·
2016
·Vol. 11
·期 2
·e0149032
·
IF 3.057
Zhao Gao,Yu Han,Yunhui Hu,Xiaoyan Wu,Yongbin Wang,Xiaoqun Zhang,Jinjuan Fu,Xue Zou,Jun Zhang,Xiongwen Chen,Pedro A. Jose,Xi Lu,Chunyu Zeng
Both oxidative stress and inflammation are involved in the pathogenesis of contrast-induced nephropathy (CIN). Epigallocatechin-3-gallate (EGCG), a purified catechin from green tea, has antioxidant and anti-inflammatory effects. However, it is unknown whether or not EGCG is effective in treating CIN. Our present study found that intravenous administration of EGCG, either before or just after the establishment of CIN, had a protective effect, determined by normalization of serum creatinine and blood urea nitrogen levels, improvement in renal histopathological scoring and alleviation of apoptosis, accompanied by decreased oxidative stress and inflammation. Because EGCG is a potent inducer of the antioxidant heme oxygenase-1 (HO-1), we studied HO-1 signaling in CIN. HO-1 levels were increased in CIN; treatment with EGCG further increased HO-1 levels, accompanied by an increase in Nrf2, a regulator of antioxidant proteins. Interestingly, blockade of HO-1 with protoporphyrin IX zinc(II) (ZnPP) prevented the protective effect of EGCG on CIN. ZnPP also blocked the ability of EGCG to increase the activity of an antioxidant (superoxide dismutase), and decrease markers of oxidative stress (myeloperoxidase and malondialdehyde) and inflammation (myeloperoxidase and IL-1β), indicating that HO-1 is the upstream molecule that regulates the EGCG-mediated protection. To determine further the role of HO-1 on the EGCG-mediated inhibition of inflammation, we studied the effect of EGCG on the NLRP3 inflammasome, an upstream signaling of IL-1β. EGCG down-regulated NLRP3 expression, which was blocked by ZnPP, indicating that HO-1 links EGCG with NLRP3. Therefore, EGCG, via up-regulation of HO-1, protects against CIN by amelioration of oxidative stress and inflammation.
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高影响因子
RSC Advances
·
2016
·Vol. 6
·期 19
·15354-15361
·
IF 3.289
Xinhuan Lu,Yang chen,Zhenshuang Zhao,Hao Deng,Dan Zhou,Changcheng Wei,Renfeng Nie,Qinghua Xia
The carbon supported 10% Ni catalysts doped with 3‰ Rh have been prepared by an impregnation method. These catalysts have been used to catalyze the one-step hydrogenation of nitrobenzene to cyclohexylamine. The results show that the 3‰ Rh–10% Ni/CSC (biocarbon) catalyst exhibits an excellent performance to achieve 100 mol% conversion of nitrobenzene and 91.6% selectivity of cyclohexylamine under reaction conditions of 3.5 MPa and 140 °C. The recycling tests reveal recyclable stability of 3‰ Rh–10% Ni/CSC. This catalyst is active for the hydrogenation of a series of electron-deficient nitrobenzenes. Some factors such as the type of carriers, the content of Ni and Rh, the type of metals and additives play important roles in controlling the selective hydrogenation.
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高影响因子
TETRAHEDRON
·
2016
·
IF 2.645
Xiong-Wei Liu,Zhen Yao,Jun Yang,Zhi-Yong Chen,Xiong-Li Liu,Zhi Zhao,Yi Lu,Ying Zhou,Yu Cao
A facile and efficient methodology was developed for the synthesis of isoxazole-fused spiropyrrolidine oxindoles 3–5 via a 1,3-dipolar cycloaddition reaction of 3-methyl-4-nitro-5-alkenyl-isoxazoles with azomethine ylides (thermally generated in situ from isatin derivatives and proline or thioproline or sarcosine). Products bearing adjacent quaternary-tertiary centers were smoothly obtained in high yields (up to 90% yield) with good diastereoselectivity (up to 20:1). Furthermore, their biological activity has been preliminarily demonstrated by in vitro evaluation against human prostate cancer cells PC-3, human lung cancer cells A549 and human leukemia cells K562 by the MTT-based assays using the commercially available standard drug Cisplatin as a positive control. These results suggested that most of isoxazole-fused spiropyrrolidine oxindoles 3–5 showed considerable cytotoxicities to these three cell lines K562, A549 and PC-3, and that isoxazole-fused spiropyrrolidine oxindoles may be potential leads for further biological screening.
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高影响因子
ENERGY & FUELS
·
2017
·
IF 3.091
Cheng Sun,Shujing Wen,Jingkai Zhao,Chongjian Zhao,Wei Li,Sujing Li,Dongxiao Zhang
In this work, the blends of 1-hydroxyethyl-3-methylimidazolium glycine ([C2OHmim][Gly]) synthesized by our laboratory and N-methyldiethanolamine (MDEA) in aqueous solution were prepared for CO2 capture, and the maximum absorption performance of the blends was obtained at the mole ratio of 8:2 of MDEA/[C2OHmim][Gly] with a total concentration of 1.0 mol L–1. CO2 loading of the blended absorbent was less adversely influenced by the temperature and O2 concentration than that of MDEA aqueous, and it had a good performance in regeneration ability. The reaction mechanism of the CO2 absorption in MDEA/[C2OHmim][Gly] was investigated by 13C nuclear magnetic resonance. CO2 first reacted with [C2OHmim][Gly] to form carbamate, and then carbamate promoted the reaction between CO2 and MDEA, which could be described as a shuttle mechanism. The kinetics of CO2 absorption was investigated in a double stirred-cell absorber at different temperatures, and some important kinetic parameters were obtained, such as the reaction rate constant (k2) and the overall rate constant (kov). Experimental results indicated that the addition of [C2OHmim][Gly] enhanced CO2 absorption of MDEA under low CO2 partial pressure, which could improve the application of MDEA in industry.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2012
·
IF 3.898
Yi Huang,Lijuan Bu,Wen Wang,Xiaoli Qin,Zou Li,Zhao Huang,Yingchun Fu,Xiaoli Su,Qingji Xie,Shouzhuo Yao
Chemical oxidation of glycoprotein-bound thiophene-3-boronic acid (TBA) monomer by relatively mild oxidant chloroplatinate in neutral aqueous solution containing uricase yielded uricase–PTBA–Pt nano bionanocomposite with high enzymatic specific activity on a platinized (Pt plate ) Au electrode, which was then covered by an outer-layer chitosan (CS) film to fabricate a CS/uricase–PTBA–Pt nano /Pt plate /Au electrode. This electrode exhibited a linear amperometric response to uric acid concentration from 5 μM to 1.2 mM with a sensitivity of 134 μA mM −1 cm −2 , a limit of detection ( S / N = 3) of 1 μM, and excellent operation/storage stability, which also worked well in serum samples.
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高影响因子
ELECTROCHIMICA ACTA
·
2015
·
IF 4.504
Youqin Lu,Xilin Wang,Min Wang,Lingqian Kong,Jinsheng Zhao
This paper reports the synthesis and characterization of two none-noble oxygen reduction reaction (ORR) catalysts including the CuPPyPhen/C and the FePPyPhen/C. The catalysts were prepared by covalently bonding of the 1,10-phenanthroline metal complexes with the poly(pyrrole-3-carboxylic acid)/C composite, which was prepared with an oxidative chemical method of polymerization of pyrrole-3-carboxylic acid on the carbon surface. Both catalysts were characterized using electrochemical techniques such as cyclic voltammetry (CV), rotating disk electrode (RDE), as well as rotating ring disk electrode (RRDE) to quantitatively obtain ORR kinetic constants and the reaction mechanisms. The results of the data showed that both catalysts underwent a predominant four-electron pathway for the reduction of oxygen, with the CuPPyPhen/C catalyst exhibited a better ORR activity and stability than that of the FePPyPhen/C and PPyPhen/C catalyst, suggesting that the ORR activity of the catalyst depend on the species of the metal ions which plays a key role to enhance the eletrocatalytic activity and accelerate the step for ORR. The physical morphologies and the surface chemical compositions of the catalysts were examined by scanning electron microscopy and X-ray photoelectron spectroscopy, respectively.
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