ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Mengyuan Yin,Yaxing Zhang,Hanxue Liang,Chang Liu,Yanhui Bi,Juanjuan Sun,Weiwei Guo
Stimuli-responsive DNA hydrogels have shown great potential in sensing applications due to their attractive properties such as programmable target responsiveness, excellent biocompatibility, and biodegradability. In contrast to the extensively developed DNA hydrogel sensing systems based on the stimuli-responsive hydrogel-to-solution phase transition of the hydrogel matrix, the quantitative sensing application of DNA hydrogels exhibiting smart shape deformations has rarely been explored. Moreover, bulk DNA hydrogel-based sensing systems also suffer from high material cost and slow response. Herein, free-standing bilayer polyacrylamide/DNA hybrid hydrogel films with programmable responsive properties directed by the sequence of functional DNA units have been constructed. Compared with bulk DNA hydrogels, these DNA hydrogel films with a thickness at the micrometer scale not only greatly reduce the consumption of DNA materials but also facilitate the mass transfer of biomacromolecular substances within the hydrogel network, thus favoring their sensing applications. Therefore, a target-responsive smart DNA hydrogel film-based sensor system is further demonstrated based on the large amplitude macroscopic shape deformation of the film as a visual signal readout. As a proof of concept, Pb2+ or UO22+ ion-responsive DNA units were introduced into the active layer of the bilayer hydrogel films. In the presence of Pb2+ or UO22+ ions, the occurrence of a cleavage reaction within the DNA units leads to the release of DNA segments from the hydrogel film, inducing a dramatic shape deformation of the film, and thus sensing of Pb2+ or UO22+ ions with high specificity is achieved based on measuring the bending angle changes of these smart free-standing films. These smart DNA hydrogel film sensors with target-programmable responsiveness, simple operation, and ease of storage may hold promise for future rapid on-site testing applications.
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高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 9
·1-13
·
IF 5.3
He Linxin,Wei Yuxin,Chen Cizhi,Li Chuanhai,Xu Ying,Chen Mei
A sensitive dual-target electrochemical biosensor was developed integrating circular bipedal DNA walkers with a three-dimensional hollow carbon nanospheres-tetrametallic nanoparticle (3D HCNs-TT NPs) modified platform, enabling concurrent detection of two critical epidermal growth factor receptor (EGFR) exon 19 mutations: E746_A750 deletion (Mut DNA A) and L747_S752delinsS (Mut DNA B). The circular bipedal DNA walkers address the limitations of low amplification efficiency in traditional single-legged DNA walkers and mitigate the sensitivity reduction caused by excessively long walking strands. Additionally, we used the 3D HCNs-TT NPs nanocomposite materials to modify the electrodes, which significantly increased the electron transfer rate and provided more active sites for molecular probe loading, thereby further enhancing the performance of the detection platform. This biosensor demonstrated good performance, with detection limits as low as 3.4 fM (Mut DNA A) and 2 fM (Mut DNA B) (S/N = 3). The designed sensor was applied for detection using clinical samples, including cells with gene mutations and diluted human serum, and these results confirm the sensor’s potential for clinical translation, offering a potential tool for early non-small cell lung cancer (NSCLC) diagnosis and personalized tyrosine kinase inhibitors (TKI) therapy guidance. Graphical abstract
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Sili Chen,Xiangyu Ding,Tao Zheng,Hao Deng,Qiaoyuan Yang
Benzo[ghi]perylene (BghiP), a prevalent high-molecular-weight (HMW) polycyclic aromatic hydrocarbon (PAH) with significant environmental exposure, poses potential health risks that remain inadequately characterized. This study investigated the respiratory toxicity of BghiP exposure and its underlying mechanisms in vitro (human bronchial epithelial cells BEAS-2B and 16HBE) and in vivo (mouse model). Chronic exposure to environmentally relevant concentrations of BghiP (500 ng/mL for 2 weeks in vitro ; 500 µg/m 3 for 4 weeks in vivo ) significantly reduced cell proliferation, induced apoptosis and cell cycle arrest, and caused DNA damage in BEAS-2B and 16HBE cells. In vivo, BghiP exposure increased lung coefficients, induced pathological alterations, and triggered pulmonary inflammation. Mechanistically, BghiP exposure upregulated the expression of cytochrome P450 (CYP1A1), which subsequently promoted the transcription of chimeric RNA RPL17-C18orf32. We identified that the CYP1A1/RPL17-C18orf32 axis plays a pivotal role in mediating BghiP's toxic effects. Knockdown of either CYP1A1 or RPL17-C18orf32 attenuated BghiP-induced cytotoxicity, DNA damage, and cellular dysfunction, while overexpression of RPL17-C18orf32 exacerbated these detrimental effects. Co-manipulation experiments further confirmed that CYP1A1 acts upstream to regulate RPL17-C18orf32 expression. This study is the first to provide evidence that the CYP1A1/RPL17-C18orf32 axis is a critical mediator of BghiP-induced respiratory toxicity and DNA damage. Our findings systematically elucidate the cytotoxic and genotoxic effects of BghiP, introducing the novel concept of chimeric RNA involvement in PAH toxicity mechanisms and offering a new perspective for understanding the health impacts of environmental pollutants.
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BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Jingqi Tian,Qian Liu,Jinle Shi,Jianming Hu,Abdullah M. Asiri,Xuping Sun,Yuquan He
Considerable recent attention has been paid to homogeneous fluorescent DNA detection with the use of nanostructures as a universal “quencher”, but it still remains a great challenge to develop such nanosensor with the benefits of low cost, high speed, sensitivity, and selectivity . In this work, we report the use of iron-based metal–organic framework nanorods as a high-efficient sensing platform for fluorescent DNA detection. It only takes about 4 min to complete the whole “mix-and-detect” process with a low detection limit of 10 pM and a strong discrimination of single point mutation . Control experiments reveal the remarkable sensing behavior is a consequence of the synergies of the metal center and organic linker. This work elucidates how composition control of nanostructures can significantly impact their sensing properties, enabling new opportunities for the rational design of functional materials for analytical applications.
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高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2018
·Vol. 18
·期 6
·3825-3831
·
IF 1.354
Chen Jian,Wang Jun-Feng,Wu Xue-Zhong,Rong Zhen,Dong Pei-Tao,Xiao Rui
We developed a high-performance surface-enhanced Raman scattering (SERS) sensing platform that can be used for specific and sensitive DNA detection. The SERS platform combines the advantages of Au film over nanosphere (AuFON) substrate and Ag@PATP@SiO2 SERS tag. SERS tag-on-AuFON
is a sensing system that operates by the self-assembly of SERS tag onto an AuFON substrate in the presence of target DNAs. The SERS signals can be dramatically enhanced by the formation of “hot spots” in the interstices between the assembled nanostructures, as confirmed by finite-difference
time-domain (FDTD) simulation. As a new sensing platform, SERS tag-on-AuFON was utilized to detect Staphylococcus aureus (S. aureus) DNA with a limit of detection at 1 nM. A linear relationship was also observed between the SERS intensity at Raman peak 1439 cm−1 and
the logarithm of target DNA concentrations ranging from 1 μM to 1 nM. Besides, the sensing platform showed good homogeneity, with a relative standard deviation of about 1%. The sensitive SERS platform created in this study is a promising tool for detecting trace biochemical molecules
because of its relatively simple and effective fabrication procedure, high sensitivity, and high reproducibility of the SERS effect.
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ANALYTICAL SCIENCES
·
2017
·Vol. 33
·期 5
·585-590
·
IF 1.228
Zhu Xiaoqian,Huang Min,Li Jiao,He Hanping,Zhang Xiuhua,Wang Shengfu
In this paper, a novel electrochemical sensor was developed for the rapid detection of G-G mismatched DNA based on hexaammineruthenium(III) chloride ([Ru(NH 3 ) 6 ]C l3 ) as a redox indicator. The sensor platform was constructed by immobilizing small molecules (NC-linker) on the gold electrode via amide bonds. The as-prepared NC-linker as the nucleic acids recognition molecule can interact with the G base of DNA. After the sensor was incubated with G-G mismatched DNA, the double-stranded DNA (dsDNA) acted as carriers of the signal tags-[Ru(NH3)6]Cl3, which resulted in a remarkable electrochemical signal. More binding of [Ru(NH 3 ) 6 ]C l3 led to increases of the electrochemical signal. Other mismatched DNA produced only a low response, as well as complementary DNA. Thus G-G mismatched DNA can be easily discriminated from other mismatched and complementary DNA based on the sensor. Furthermore, the method was simple, rapid and repeatable for the detection of G-G mismatched DNA. The selective detection of target dsDNA was achieved by a relative current ratio of the target and control DNA. These results demonstrated that this strategy could provide great promise for the rapid and specific detection of other sequence-specific DNA.
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ANALYTICAL BIOCHEMISTRY
·
2022
·
IF 3.191
Huikai Ma,Ping Wang,Yaoyao Xie,Jinghan Liu,Wei Feng,Sanqiang Li
A novel ratiometric electrochemical aptasensor was constructed for the detection of carcinoembryonic antigen (CEA) based on a hairpin DNA (hpDNA) probe and exonuclease Ⅰ (Exo Ⅰ)-assisted target recycling amplification strategy. A thiolated methylene blue (MB)-labeled hpDNA as the internal control element was fixed on the surface of the gold nanoparticles (AuNPs)-modified gold electrode (AuE) through Au–S bonds. A ferrocene (Fc)-modified aptamer DNA (Fc-Apt) was partially hybridized with hpDNA to form a Fc-Apt/hpDNA duplex. Due to the specific recognition of Fc-Apt to CEA, the presence of CEA caused dissociation of Fc-Apt from the duplex, and further triggered the degradation process of Exo Ⅰ and recycling of CEA. Hence, the Fc tags were released from the electrode surface and the oxidation peak current of Fc (I Fc ) decreased while that of MB (I MB ) remained stable owing to the distance between MB tags and the electrode unchanged. A linear relationship was observed between I Fc /I MB and the logarithm of CEA concentration from 10 pg mL −1 to 100 ng mL −1 with a detection limit of 1.9 pg mL −1 . Moreover, the developed aptasensor had been applied to detect CEA in diluted human serum with satisfactory results, indicating its great potential in practical applications.
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MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Wanling Cui,Xiaoyang Fan,Congcong Shen,Qingshuai Chen,Xiumei Zhang,Enguang Lv,Chonghui Li,Qiang Li,Rui Wang,Shicai Xu,Jihua Wang
T4 polynucleotide kinase (T4 PNK) and T4 DNA ligase are two important repair enzymes that play a significant role in maintaining the stability and integrity of genome. Here, we proposed a multiple primers-mediated exponential rolling circle amplification strategy for label-free and highly sensitive fluorescent analysis of T4 PNK and T4 DNA ligase activity. Firstly, we designed a target strand that contained two C-rich sequences, a complementary sequence of the primer, and three cleavage sites of Nt.BbvCI scattered among the three sequences. The 5′-hydroxyl terminus of target strand was catalyzed by T4 PNK to form a phosphorylated target strand. After the phosphorylated target strand was combined with the primer, T4 DNA ligase catalyzed the formation of circular DNA. Then, the primer triggered an exponential rolling circle amplification reaction along the circular DNA template, exponentially generating G-rich sequences. N-methyl mesoporphyrin IX as signal molecules specifically bound to the G-quadruplexs folded by G-rich sequences, generating a detectable fluorescent signal. Benefiting from exponential signal growth, this strategy showed extremely high detection sensitivity with detection limits of 8.11 × 10 −5 U mL −1 of T4 PNK and 3.07 × 10 −4 U mL −1 of T4 DNA ligase, respectively. In addition, the proposed strategy exhibited excellent inhibitor evaluation effects for two repair enzymes, and could detect T4 PNK in HeLa cell lysate. The obtained results showed that the proposed strategy had excellent application prospects in disease diagnosis and treatment evaluation.
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Small
·
2018
·Vol. 14
·期 40
·1802292
·
IF 9.598
Keying Zhang,Shuting Song,Shan Huang,Lin Yang,Qianhao Min,Xingcai Wu,Feng Lu,Jun-Jie Zhu
Intracellular microRNAs imaging based on upconversion nanoprobes has great potential in cancer diagnostics and treatments. However, the relatively low detection sensitivity limits their application. Herein, a lock-like DNA (LLD) generated by a hairpin DNA (H1) hybridizing with a bolt DNA (bDNA) sequence is designed, which is used to program upconversion nanoparticles (UCNPs, NaYF 4 @NaYF 4 :Yb, Er@NaYF 4 ) and gold nanoparticles (AuNPs). The upconversion emission is quenched through luminescence resonance energy transfer (LRET). The multiple LLD can be repeatedly opened by one copy of target microRNA under the aid of fuel hairpin DNA strands (H2) to trigger disassembly of AuNPs from the UCNP, resulting in the lighting up of UCNPs with a high detection signal gain. This strategy is verified using microRNA-21 as model. The expression level of microRNA-21 in various cells lines can be sensitively measured in vitro, meanwhile cancer cells and normal cells can be easily and accurately distinguished by intracellular microRNA-21 imaging via the nanoprobes. The detection limit is about 1000 times lower than that of the previously reported upconversion nanoprobes without signal amplification. This is the first time a nonenzymatic signal amplification method has been combined with UCNPs for imaging intracellular microRNAs, which has great potential for cancer diagnosis.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 8
·1-7
·
IF 5.705
Meng Fanyu,Xu Yuanyuan,Dong Wenfei,Tang Yuguo,Miao Peng
The paper describes a voltammetric method for the quantitation of the activity of telomerase extracted from cancer cells. A thiolated single-stranded telomerase substrate primer was firstly immobilized on a gold electrode. In the presence of a mixture of telomerase and deoxynucleotide triphosphates, the primer becomes elongated and contains repetitive nucleotide sequences (TTAGGG) n . After hybridization with blocker DNA, gold nanoparticles are added and captured by the elongated single-stranded DNA. This reduces the charge transfer resistance of the gold electrode. The telomerase activity is then quantified via differential pulse voltammetry, typically at 0.12 V (vs. SCE). The method is PCR-free, rapid, and convenient. It was applied to the detection of HeLa cells via the telomerase activity of lysed cells. The detection range was from 500 to 50,000 cells/mL and the detection limit was as low as 500 cells/mL.
A telomerase substrate (TS) primer is immobilized on a gold electrode as the sensing interface to detect the activity of telomerase extracted from cancer cells. Unmodified gold nanoparticles (AuNPs) are utilized which change the electrochemical responses.
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高影响因子
Journal of Materials Chemistry B
·
2015
·Vol. 3
·期 41
·8180-8187
·
IF 4.726
Ke-Jing Huang,Yu-Jie Liu,Qiu-Fen Zhai
Recently, two-dimensional layered transition metal dichalcogenide materials have been very fascinating in electrochemical fields due to their unique electronic and electrochemical properties. In the present work, a novel VS2–graphene (VS2–GR) composite is firstly prepared by a facile one-step hydrothermal method. A sensitive, universal and label-free electrochemical aptamer-based sensor (aptasensor) is then designed to detect the platelet-derived growth factor BB (PDGF-BB) based on VS2–GR coupled with Exo III-aided autocatalytic signal amplification. In the absence of PDGE-BB, the aptamer hybridized with the complementary DNA (cDNA) and the single-strand signal DNA labeled with biotin at 5′ end cannot be cleaved by the Exo III. When the above mixture is applied on the tetrahedron-structured probe (T-DNA) modified AuNP/VS2–GR electrode, signal DNA hybridizes with the protruding of T-DNA and a large number of avidin-HRP are adsorbed on the modified electrode by the specific binding of avidin–biotin, which leads to a strong current response by the catalysis of HRP to the mixture of hydrogen peroxide and hydroquinone (H2O2 + HQ). However, in the presence of PDGF-BB, the aptamer is preferentially associated with PDGF-BB, which facilitates the formation of double-strand DNA between cDNA and signal DNA. The duplex DNA is digested by Exo III from the 3′ blunt terminus of signal DNA and the cDNA is released. The liberative cDNA then reacts with the remaining signal DNA in the solution to perform a new cleavage process. Finally, the cyclic hybridization–hydrolysis process leads to a great decrease of biotin on the electrode, thus reducing the quantity of signal molecule HRP and causing an obvious decline of current response. This proposed protocol exhibits high specificity, good sensitivity, and good stability for PDGF-BB detection. Moreover, the aptasensor can be applied as a universal assay for the detection of various targets including proteins, DNAs, and metal ions.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 12
·1-9
·
IF 5.479
Li Hui,Lu Yuexiang,Pang Jiawei,Sun Jingwei,Yang Fengyi,Wang Ziyi,Liu Yueying
A fluorometric and colorimetric method are described for the determination of hydrogen peroxide and glucose by integrating copper nanoclusters (CuNCs) into a Fenton-like reaction. The mechanism mainly depends on the fast formation of long-strand DNA-templated CuNCs with strong red fluorescence (with excitation/emission maxima at 340/640 nm) in the absence of H 2 O 2 . The DNA can be cleaved into short-oligonucleotide fragments by hydroxy radicals as formed in the Ce(III)-triggered Fenton-like reaction in the presence of H 2 O 2 . As a result, short-strand DNA loses the ability as a template for the formation of CuNCs. This leads to a decrease of fluorescence. The colorimetric assay, in turn, is based on the oxidation of colorless Ce(III) ions to the distinctly yellow Ce(IV) ions (with an absorption maximum at 400 nm) by H 2 O 2 . Compared with those assays based on the use of enzyme mimics, this method does not require any chromogenic substrates such as ABTS and TMB. Based on the dual-signal readout platform, we successfully achieved the detection of H 2 O 2 and glucose. LODs are as low as 0.266 μM and 2.92 μM. The methods were applied to the sensitive determination of glucose by using glucose oxidase (GOx) which catalyzes the oxidization of glucose to produce H 2 O 2 . The practical application was demonstrated by determination of glucose in human serum, with apparent recoveries of 98.4–101.9% and 99.1–105.6%, respectively. The concentration of glucose ranges from 1 to 500 μM and 50 to 600 μM based on the dual-signal readout platform, respectively. This fluorometric and colorimetric dual-mode strategy will pave a new avenue for constructing effective assays for H 2 O 2 -related analytes in biochemical and clinical applications.
Schematic representation of a fluorometric and colorimetric dual-readout strategy for the sensitive determination of hydrogen peroxide and glucose. The assay has been designed by integrating copper nanoclusters into a Ce(III)-triggered Fenton-like reaction.
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