CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Xia Yang,Jing Wang,Zhikai Ding,Qingchuan Lin,Liangang Zhuo,Wei Liao,Yan Zhao,Yue Feng,Yue Chen,Hongyuan Wei,Yuchuan Yang
Hyaluronan (HA) have been widely used as the ideal biomaterials. It is important to understand their degradation and distribution for better optimization. From a new aspect of using radiotracers, we designed the HA-tyramine-bisphosphonate derivative for dual-labelling with two radionuclides ( 99m Tc and 131 I) simultaneously for in vitro and in vivo tracking. This dual-radiolabelled HA derivative can still be non-covalently crosslinked by hydroxyapatites to form injectable gel. The excellent properties of the gel, such as robust, biodegradable, and self-healing capacity were maintained. We firstly proved the possibility to distinguish different radionuclides in the degraded gel using the high-resolution gamma-ray spectrometry. The radiolabelled gel showed lower toxicity than pure hydroxyapatites against various cell lines, while the in vivo results proved that the 99m Tc/ 131 I-labelling of the gel was safe and stable enough for imaging and quantitatively tracking. The present method can also be applied for the development of dual-radiolabelled gels from other polysaccharides.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Proteomics
·
2019
·
IF 3.537
Zhuo-Qing Li,Ling-Li Wang,Jing Zhou,Xian Zheng,Yan Jiang,Ping Li,Hui-Jun Li
Dictamnine (DTN), a major furoquinoline alkoloid from Dictamni Cortex, was reported to induce hepatotoxicity. However, the underlying mechanism is unclear. In the present study, integrated transcriptomic and metabolomics analysis of mouse liver was performed in combination with serum biochemical and histopathological evaluation to investigate the potential mechanism. The results suggested that 640 mg/kg DTN significantly increased serum alanine transaminase and aspartate transaminase levels and induced serious cellular degeneration, with no changes occurring with 4 mg/kg DTN. Integrated analysis suggested that the metabolism of xenobiotics by cytochrome P450 , drug metabolism-other enzymes , bile secretion and glutathione metabolism were the major metabolic pathways involved in DTN-induced hepatotoxicity. Notably, 640 mg/kg DTN exposure increased hepatic GSH, GSH peroxidase , superoxide dismutase and malondialdehyde, and decreased ROS , together with altered expression of Idh2 and Nedd9. Representative genes, including Mup12 , Lipc, NTCP , MRP3, MRP4, CYP2E1 , CYP2D9 and UGT1A9 , in altered pathways were verified through PCR and Western blot . Collectively, the combined strategy of transcriptomics and metabolomics profiling could facilitate a better understanding for the discovery of metabolic pathways and that oxidative damage, ABC transporters and lipid metabolism might be the mechanisms linked to DTN-induced hepatotoxicity in mice. Significance Dictamnine (DTN) was reported to induce hepatotoxicity. Nevertheless, the underlying mechanism is unknown. This study is the first to utilize integrated transcriptomics and metabolomics in combination with general toxicity evaluation to characterize the potential molecular mechanism in DTN-induced hepatotoxicity in mice. We found that acute exposure to higher dose of DTN induced hepatocellular liver injury with more changes in biochemical parameters, genes and metabolites. Gene expression and metabolite profiles were more sensitive than general toxicity studies for detecting earlier hepatotoxicity. Integrated analysis suggested that oxidative damage, ABC transporters and lipid metabolism were closely correlated with DTN-induced hepatotoxicity. Overall, our results provide insights into the mechanism responsible for DTN-induced hepatotoxicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 24
·3909-3916
·
IF 5.047
Huan Feng,Yue Wang,Jianping Liu,Zhiqiang Zhang,Xinyi Yang,Rong Chen,Qingtao Meng,Run Zhang
Hypochlorous acid (HOCl) is closely related to human health and is widely used in our daily lives. Accordingly, detection of HOCl in live organisms and natural water samples is significantly important to human and animal health. In this paper, a novel chromogenic and fluorogenic probe (PI) was prepared for HOCl detection with high sensitivity and specificity. PI exhibits weak fluorescence emission owing to the intramolecular charge transfer (ICT) process from the carbazole to indandione unit. The oxidative cleavage of the alkene unit of PI induces the interruption of its π-conjugation, resulting in colorimetric and fluorogenic responses towards HOCl in PBS buffer. The detection limit of PI for HOCl was calculated to be as low as 24 nM. The fluorescence response of PI towards HOCl was found to be completed within 1 minute. PI exhibits high selectivity towards HOCl and low cytotoxicity, which allows it to be used as a fluorescent imaging probe for visualisation of exogenous and endogenous HOCl in live adult zebrafish and nude mice. Moreover, “naked-eye” analysis of the HOCl concentration in several natural water samples has been realized using PI-based test plates.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Huihui Liu,Yajing Li,Mengwei Yuan,Genban Sun,Huifeng Li,Shulan Ma,Qingliang Liao,Yue Zhang
Electrospinning and annealing methods are applied to prepare cobalt nanoparticles decorated in N-doped carbon nanofibers (Co/N-C NFs) with solid and macroporous structures. In detail, the nanocomposites are synthesized by carbonization of as-electrospun polyacrylonitrile/cobalt acetylacetonate nanofibers in an argon atmosphere. The solid Co/N-C NFs have lengths up to dozens of microns with an average diameter of ca. 500 nm and possess abundant cobalt nanoparticles on both the surface and within the fibers, and the cobalt nanoparticle size is about 20 nm. The macroporous Co/N-C NFs possess a hierarchical pore structure, and there are macropores (500 nm) and mesopores (2–50 nm) existing in this material. The saturation magnetization (Ms) and coercivity (Hc) of the solid Co/N-C NFs are 28.4 emu g–1 and 661 Oe, respectively, and those of the macroporous Co/N-C NFs are 23.3 emu g–1 and 580 Oe, respectively. The solid Co/N-C NFs exhibit excellent electromagnetic wave absorbability, and a minimum reflection loss (RL) value of −25.7 dB is achieved with a matching thickness of 2 mm for solid Co/N-C NFs when the filler loading is 5 wt %, and the effective bandwidth (RL ≤ −10 dB) is 4.3 GHz. Moreover, the effective microwave absorption can be achieved in the whole range of 1–18 GHz by adjusting the thickness of the sample layer and content of the dopant sample.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Ji Xia,Yong Fang,Yi Shi,Xinchun Shen,Jian Wu,Minhao Xie,Peng Li,Fei Pei,Qiuhui Hu
The bioavailability and oxidative damage toxicity of lead (Pb) in seven food matrices, including rice, milk, tomato, garlic, apple, kelp and pork, were determined using an in vitro digestion/Caco-2 cell model and a rat pheochromocytoma (PC12) oxidative damage model. Results showed that Pb bioaccessibility and bioavailability in the apple and kelp groups were significantly lower than other food matrix groups, with corresponding values of 11.05–28.31% and 1.57–8.81%, respectively. Oxidative damage assays showed that digestion products of apple polyphenol extract, which was selected from seven food matrices, could increase the oxidation resistance and the levels of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and acetyl cholinesterase (AChE) by 32.23%, 39.02%, 27.14% and 30.90%, respectively. Additionally, malondialdehyde (MDA) and reactive oxygen species (ROS) levels could be decreased by 59.66% and 40.21%, respectively. In conclusion, phenolics were an important food matrix that could decrease the bioavailability and oxidative damage of Pb.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 13
·11023-11030
·
IF 3.201
Lulu Liang,Li Nie,Minjuan Jiang,Fusheng Bie,Linjun Shao,Chenze Qi,X. Man Zhang,Xuejing Liu
Chitosan composite superfine fibers with a diameter of 321 ± 99 nm were prepared by electrospinning with PEO as the co-spinning polymer and itaconic acid as the in situ cross-linking agent. Itaconic acid was homogeneously dispersed in chitosan fibers and the in situ cross-linking endowed the prepared chitosan composite fibers with improved solvent resistance, thermostability and mechanical strength. Palladium species immobilized on these annealed chitosan fibers can efficiently catalyze the reduction reaction of 4-nitrophenol as well as the Mizoroki–Heck reaction of aromatic iodides with n-butyl acrylate in an aqueous medium. Furthermore, the larger fiber structure can facilitate the recovery and reuse of the palladium catalyst. The remarkable catalytic performance and easy recovery make this novel fiber-supported palladium catalyst hold great potential applications in green chemistry.
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 9
·2329
·
IF 3.098
Shao-Nan Wang,Yong-Sheng Ding,Xiao-Jie Ma,Cheng-Bowen Zhao,Ming-Xuan Lin,Jing Luo,Yi-Nan Jiang,Shuai He,Jian-You Guo,Jin-Li Shi
Zhi zhu xiang(ZZX for short) is the root and rhizome ofValeriana jatamansiJones, which is a Traditional Chinese Medicine (TCM) used to treat various mood disorders for more than 2000 years, especially anxiety. The aim of the present work was to identify the bioactive chemical markers inZhi zhu xiangimproving anxiety in rats by a fingerprint-efficacy study. More specifically, the chemical fingerprint of ZZX samples collected from 10 different regions was determined by High Performance Liquid Chromatography (HPLC) and the similarity analyses were calculated based on 10 common characteristic peaks. The anti-anxiety effect of ZZX on empty bottle stimulated rats was examined through the Open Field Test (OFT) and the Elevated Plus Maze Test (EPM). Then we measured the concentration of CRF, ACTH, and CORT in rat’s plasma by the enzyme-linked immune sorbent assay (ELISA) kit, while the concentration of monoamine and metabolites (NE, DA, DOPAC, HVA, 5-HT, 5-HIAA) in the rat’s cerebral cortex and hippocampus was analysed by HPLC coupled with an Electrochemical Detector. At last, the fingerprint-efficacy study between chemical fingerprint and anti-anxiety effect of ZZX was accomplished by partial least squares regression (PLSR). As a result, we screened out four compounds (hesperidin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C) as the bioactive chemical markers for the anti-anxiety effect of ZZX. The fingerprint-efficacy study we established might provide a feasible way and some elicitation for the identification of the bioactive chemical markers for TCM.Keywords:anti-anxiety;Valeriana jatamansiJones;fingerprint-efficacy relationship;PLSR
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Junhe Lu,Peizeng Yang,Wei Dong,Yuefei Ji,Qingguo Huang
Benzophenone-4 (BP4) is UV-filter that is widely in sunscreens and cosmetics to prevent skin damage from sunlight exposure. Washing off of BP4 from the human body in swimming pools represents a direct source of BP4 in the environment. In this study, we investigated the transformation of BP4 by free chlorine, chloramine, and UV/chlorine, which can be found in swimming pool water. It was found that BP4 can be rapidly removed by both chlorine and chloramine treatment, but only chlorination led to appreciable formation of chlorinated disinfection by-products (DBPs), such as dichloroacetic acid and trichloroacetic acid . However, chloramination of BP4 resulted in higher levels of chlorinated intermediates, which were relatively recalcitrant to further chlorination. The UV/chlorine process was significantly more efficient than the chlorine treatment alone for BP4 removal and resulted in trace chlorinated intermediates and formation of DBPs. Radical scavenger tests revealed that the removal of BP4 in the UV/chlorine process was mostly ascribed to reactions with the Cl and OH generated from HClO photolysis. The presence of methanol as a radical scavenger resulted in incomplete removal of BP4 in the UV/chlorine process and enhanced the formation of DBPs. The increase in DBP formation was because the residual BP4 can be exited to a triplet state ( 3 BP4 ∗ ) upon UV irradiation. 3 BP4 ∗ subsequently reacted with O 2 to form 1 O 2 , a reactive oxygen species that generated DBPs by reacting with chlorinated intermediates in water. All benzophenone-type UV-filters may have similar photochemical activity and thus influence the transformation of other organics in sunlit surface water environments.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Sciences
·
2018
·
IF 3.12
Yanjun Zhang,Changwei Zhao,Shaofeng Zhang,Ling Yu,Jiding Li,Li-an Hou
The lack of fresh water in the world makes the search for an effective method to decontaminate water an urgent priority. An important step is to remove different multivalent ions in salt treatment. Nanofiltration (NF) has been used for treating water containing different kinds of salts. In this work, sulfonate group-modified graphene oxide (SGO) was prepared, and added during the interfacial polymerization (IP) reaction to prepare SGO-modifiedNF membranes (PA-SGO). The chemical composition, structure and surface properties of PA and PA-SGO membranes were characterized by FT-IR, XPS, SEM, AFM, contact angle and zeta potential measurements. Their water flux , salt rejection and anti-fouling abilities were investigated systematically. The testing results showed that the water flux of PA-SGO (0.03% SGO) was 45.85 LMH under a pressure of 0.2 MPa, and the salt rejection varied in the order of Na 2 SO 4 (98.99%) > MgSO 4 (91.25%) > MgCl 2 (42.27%) > NaCl (21.96%). An anti-fouling experiment indicated that the PA-SGO membrane had good anti-fouling properties because of its decreased roughness and increased hydrophilicity and electronegativity. The PA-SGO membrane has good potential for use in removing salt ions from water.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2018
·
IF 3.129
Kaiqiang Wang,Da-Wen Sun,Qingyi Wei,Hongbin Pu
Emulsion is a commonly investigated bioactive loaded delivery system. The bioactive content and its location in oil phase primarily determine the quality and chemical stability of emulsion. In this study, Raman microspectroscopy was used to quantify α-tocopherol and to visualize its distribution in oil-in-water emulsion stabilized by whey protein isolates. Results suggested that α-tocopherol contents (25–300 g/kg) in corn oil with the integrating Raman intensity at 481.9 and 588.6 cm −1 showed determination coefficient ( R 2 ) of 0.98 and 0.99, and limit of detection of 5.1 and 21.2 g/kg, respectively. For detecting α-tocopherol in emulsions, the relative standard deviation values from Raman method using intensities at 481.9 cm −1 and 588.6 cm −1 were in the ranges of 4%–16% and 2%–6%, respectively. The developed Raman method provided correlative results with those of HPLC method ( R 2 = 0.99). Moreover, Raman chemical imaging depicted the non-homogeneous TOC distribution within oil droplets, where TOC had the trend of migrating to the interface of oil and water. This study provided a novel approach for functional emulsion analysis, which may serve the basis for designing stable and controllable release of emulsion systems in future.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2018
·Vol. 134
·期 2
·1071-1082
·
IF 2.209
Zhou Shaojie,Qian Yi,Chen Xilei,Li Long
Layered double hydroxides-silicon dioxide hybrids (LDH-Si) were in situ synthesized by a co-precipitation method. Flame-retardant, smoke suppression and thermal degradation properties of the LDH-Si in ethylene-vinyl acetate (EVA) composites were studied using cone calorimeter test (CCT), limiting oxygen index (LOI), smoke density test (SDT), and thermogravimetry–Fourier transform infrared spectrometry. The CCT results indicated that the peak heat release rate and peak smoke production rate of the EVA/LDH-Si composite with 2.5 mass% SiO 2 were decreased by 28.5 and 21.9% compared to EVA/LDH composite, respectively. The LOI results showed that a suitable addition of SiO 2 can significantly enhance the LOI values of the EVA composites. It was obtained from SDT that SiO 2 could help smoke suppression. The EVA/LDH-Si composites possessed a higher thermal stability than the EVA/LDH composites at high temperatures.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
·
2018
·
IF 3.165
Yueyue Wu,Xinmei Huang,Min Yang,Jiong Xu,Zaoping Chen,Zhiyan Yu,Jun Liu
The current investigation was performed for the detailed analysis of protective effect of biofabricate berberine coated nano‑silver ameliorate (BBR-AgNPs) on acetaminophen (APAP) induced hepato-renal damages in diabetic rats by blood biochemistry , tissue biochemistry , histopathological and immunohistochemical analysis. The spherical shaped BBR-AgNPs were synthesized by the Biofabrication technique and its physico-chemical characterizations done by different spectroscopic (UV–vis spectrophotometer , XRD spectroscopy, FTIR spectroscopy EDAX & DLS analyses) and microscopic (FE-SEM) techniques. The diabetic developed rats were administrated with APAP (2.0 g/5 mL/kg) and scrutinize its hepato-renal injuries. The synthesized BBR-AgNPs (75 mg/kg p.o) was administrated orally to the APAP-induced diabetic rats. The result of biochemical markers and lipid peroxidation were significantly (P ˂ 0.05) increased in APAP-induced diabetic rats but decreased the level of antioxidants (P ˂ 0.05), which results obtained in liver and kidney compared to the control group. Immunohistochemical studies result showed that the APAP-induced diabetic rats expressed a high immunoreactivity of nuclear transcription factor (NF-kB). Whereas, the acetaminophen-induced diabetic rats were treated with BBR-AgNPs renovated the changes in the above parameters analyzed. The results of the study clearly indicated that the BBR-AgNPs possess the antioxidant properties as well as anti-diabetic effects, furthermore, the acetaminophen-induced liver and kidney damage was probably inhibited by the inhibition of proinflammatory factor & NF-kB factors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）