Food & Function
·
2022
·Vol. 13
·期 11
·6129-6138
·
IF 6.317
Weiwei Li,Di Liu,Laicui Song,Hao Li,Shijie Dai,Ya Su,Qianjin Li,Jianlin Li,Tiesong Zheng
Surface modified pH-responsive porous silicon (PSi) carriers were developed for efficient delivery of lutein. PSi particles were prepared by the electrochemical etching method and modified with two chemical groups: hydroxyl and octadecyl silane, respectively. Chitosan (CS) was used for coating of PSi to ensure pH-responsive release. The loading conditions, release properties, cytotoxicity and toxicity were investigated. The highest loading percentage of lutein could be obtained with oxidized PSi and the structure of the microparticles was characterized by Fourier transform-infrared spectroscopy. The surface area and pore size of the microparticles were obtained from the N2 adsorption–desorption isotherm. The CS–PSi–Lut microparticles showed the minimum surface area of 220.30 m2 g−1 and a relatively larger average pore width of 179.00 Å. In vitro release experiments showed a pH-responsive and controlled release of lutein, with the fastest release rate and highest cumulative release rate of 97% under acidic conditions (pH 5.0) within 7 h. PSi, chitosan and lutein showed synergistic toxic effects, and the CS–PSi–Lut microparticles could effectively inhibit the proliferation of HT-29 cells in a dose-dependent manner, with an inhibition rate of 77% when the lutein concentration reached 40 μg mL−1. The in vivo toxicological evaluation of CS–PSi–Lut microparticles indicated good biocompatibility in the range of experimental doses. The chitosan-coated oxidized PSi capable of delivering bioactive compounds in a targeted and controlled manner provides a novel platform for the development and application of lutein.
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Materials Science & Engineering C-Materials for Biological Applications
·
2019
·
IF 4.959
Weijun Xu,Jinlei Wang,Junmin Qian,Guanghui Hou,Yaping Wang,Lijie Ji,Aili Suo
Combination of different therapy modalities with multiple tumoricidal mechanisms has emerged as a promising anticancer strategy. Herein, we reported an aldehyde/catechol-functionalized hyaluronic acid (DAHA) and hydroxyethyl chitosan (HECS) decorated gold nanorod (GNR) platform for combined chemo-photothermal therapy of breast cancer. The DAHA was synthesized by conjugating dopamine onto oxidized hyaluronic acid. The nanoplatform was prepared by successively modifying GNR with DAHA via Au-catechol bonds, conjugating DOX onto DAHA moiety through Schiff base linkage, and coating HECS interlayer for charge-reversal and hyaluronic acid corona for tumor cell targeting. The resulting nanoplatform GNR-HA DOX CH exhibited acid-triggered surface charge-reversal and pH/NIR dual-responsive drug release behaviors. The nanoplatform could be efficiently internalized into MCF-7 breast cancer cells and displayed greater cancer cell killing than individual modalities. Therefore, polysaccharide decoration could ensure the co-delivery of GNR and DOX into cancer cells, and the developed GNR-HA DOX CH holds great potential for breast cancer treatment.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Xuegang Li,Pengqi Gong,Qiming Zhao,Xue Zhou,Yanan Zhang,Yong Zhao
A novel DNA hybridization optical fiber sensor with temperature and pH compensation for detecting a common lung cancer gene of epidermal growth factor receptor (EGFR) gene was demonstrated in this paper. The paper proposes the first research to achieve dual surface plasmon resonance (SPR) in an Fiber Bragg grating (FBG) and implement a three-parameter detection of DNA sequence , temperature and pH using a single fiber. Probe DNA sequences (pDNA) and PAA/CS, a pH-sensitive material, are immobilized on the sensor surface for EGFR gene exon-20 detection and temperature and pH monitoring simultaneously. The effectiveness of this functionalized method has been proved by using an atomic force microscope (AFM) and scanning electron microscope (SEM). The limit of detection (LOD) of EGFR gene was experimentally verified to 13.5 nM. The advantages of fast response, high sensitivity, label free, plug in, low LOD and three-parameter detection demonstrated by this sensor in the experiment are important for the field of DNA hybridization research and solving the problems of biosensor susceptibility to temperature and pH.
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ELECTROPHORESIS
·
2019
·
IF 2.754
Ping Yan,Keke Zhang,Lingyu Wang,Wenjun Tong,David D. Y. Chen
Dynamic pH junction is an online focusing method in CE based on the electrophoretic mobility difference of analytes in the sample matrix and the background electrolyte. An advantage of this method over the conventional CE is that the sensitivity can be significantly improved. By injecting a long sample plug in the capillary and focusing the analytes at the pH boundary between the background electrolyte and sample matrix, the LOD can be improved by 10–100 folds. The dynamic pH junction method can be easily coupled with ESI-MS. In this work, we used this method for the analysis of microcystins (MCs). The detection limits and dynamic ranges were studied. The separation was optimized by adjusting the injection time, and concentrations and pH values of the background electrolyte. The optimization of analyte focusing leads to enhanced detection response compared to conventional injections, achieving 200–400 fold higher averaged peak heights for four microcystin (MC) variants. More importantly, this method was successfully used for the quantitative analysis of microcystins (MCs) in crude algae samples from natural water bodies, making it promising for practical applications.
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MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2017
·Vol. 219
·期 2
·1700427
·
IF 2.5
Pan Yuan,Zheng Ruan,Le Liu,Tuanwei Li,Titao Jing,Lifeng Yan
Supramolecular self-assembly of polymeric molecules for sharp-pH-sensitive (SPS) nanoparticles is an especially important application in biology. Here, amphiphilic polypeptide copolymers are synthesized and spontaneously assembled to micelles in aqueous solution, and protonation of the tertiary amine groups in the side-chain of the copolymer under weak acid causes the micelle disassembly, which is sharp-pH-sensitive, and the responsive pHs can be adjusted by adding salts. Size and morphologic change of the polymeric micelles are characterized by means of dynamic lighting scattering, transmission electron microscope (TEM), and atomic force microscope (AFM) in sequential pH alterations. It is found that the Hofmeister ions play a key factor in controlling the subtle interaction of different noncovalent bonds among polypeptide molecules during their self-assembly or disassembly. The physical property exploration of the SPS micelles may provide a new chance for the development of dynamic, complex nanostructures in various pH-responsive applications.
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iScience
·
2024
·
IF 4.6
Meng Wang,Xiaoyu Li,Yanrui Su,Jiaoge Wu,Tian Sun,Xuan Xu,Faying Fan,Yufei Zhao,Wa Gao
Fenton or Fenton-like reactions have been widely used in various fields, including solar energy conversion to generate hydroxyl radicals, environmental remediation, biology, and life science. However, the slow Fe 3+ /Fe 2+ cycle and narrow applicable pH range still present significant challenges. Here, a heterostructured CoFe-layered double hydroxide/MoS 2 nanocomposite (CoFe-LDH/MoS 2 ) was prepared via simple electrostatic interactions. The heterostructure establishes a robust interfacial contact, leading to an abundance of exposed Mo 6+ sites. Consequently, the developed CoFe-LDH/MoS 2 +H 2 O 2 system exhibited superior performance in the degradation of tetracycline (>85%) within 60 min across a wide pH range from acidic to basic. Moreover, the CoFe-LDH/MoS 2 heterojunction catalysts exhibited exceptional resistance to common anions and efficiently degraded various organic pollutants. The mechanism study verified that the CoFe-LDH/MoS 2 had high efficiency in producing 1 O 2 and ‧OH to degrade various organic pollutants. The present study will serve as a foundation for creating efficient catalyst systems for related environmental remediation.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Xiaomei Dai,Xiaojun Liu,Lele Yang,Siyuan Yuan,Qingqing Xu,Yu Li,Feng Gao
Intracellular bacterial infections pose enormous challenges to food safety and public health. Antibiotic-based polymer prodrugs have been used to treat intracellular bacterial infection. However, the overuse of antibiotics may lead to the emergence of antibiotic resistance . In this work, we aimed to develop antibiotic-free pH-responsive polymeric prodrugs to combat intracellular S. aureus infection. Amphiphilic poly(ethylene glycol)- b -poly[(3-phenylprop-2-ene-1,1-diyl)bis(oxy)bis(enthane-2,1- diyl)diacrylate] (PEG- b -PCAE) was obtained by radical polymerization and they could self-assemble to form micelles . PEG- b -PCAE micelles could uptake by macrophage. Upon exposure to the acidic phagolysosome, PEG- b -PCAE micelles could release cinnamaldehyde (CA) through hydrolysis of the acetal linkage. PEG- b -PCAE could kill intracellular bacteria by damaging the bacterial membrane. Furthermore, PEG- b -PCAE micelles could generate reactive oxygen species (ROS) in macrophages and subsequently activate immune system to clear bacteria by inducing macrophages differentiation to M1 phenotype. PEG- b -PCAE micelles could accelerate the wound healing process of the S. aureus -infected model in vivo . It is anticipated that multifunctional antibiotic-free PEG- b -PCAE micelles with intrinsic antibacterial activities hold promise for improved outcomes in intracellular S. aureus infections.
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Polymer Chemistry
·
2017
·Vol. 8
·期 19
·2997-3005
·
IF 5.375
Meng Shan,Chu Gong,Bingqiang Li,Guolin Wu
An injectable polyethylene glycol (PEG) hydrogel was successfully prepared via borate–catechol complexation using dopamine functionalized 4-armed PEG (4-arm-PEG-DA) and phenylboronic acid modified 4-armed PEG (4-arm-PEG-PBA). The hydrogel was formed within 10 s by mixing 15 wt% 4-arm-PEG-DA with 15 wt% 4-arm-PEG-PBA buffer solutions at pH 9.0. The hydrogel was characterized by Fourier transform infrared (FT-IR) spectroscopy, rheological measurements, scanning electron microscopy (SEM) and lap shear strength tests. Furthermore, pH, glucose and dopamine responsiveness of the hydrogel based on phenylborate–catechol complexation was demonstrated by degradation experiments and drug release tests. The lap shear strength tests indicated that the hydrogel possessed the ability of self-healing which was bestowed by the phenylborate–catechol dynamic covalent bond. Moreover, the hydrogel could serve as a decent bio-adhesive due to its adhesion properties on different substrates especially the porcine skin. In vitro cytotoxicity tests demonstrated the excellent cytocompatibility of the hydrogel. The results of all the experiments indicate that our newly developed multi-functionalized hydrogel can have potential applications in biomedical fields such as drug delivery, tissue engineering, and bio-adhesives.
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EUROPEAN POLYMER JOURNAL
·
2013
·
IF 2.562
Jingquan Liu,Liang Cui,Na Kong,Colin J. Barrow,Wenrong Yang
A pH-sensitive, mechanically strong and thermally stable graphene/poly (acrylic acid) (graphene/PAA) hydrogel was prepared via reversible addition fragmentation transfer (RAFT) polymerizations in the presence of a cross-linking agent. The RAFT agent was covalently coupled onto graphene basal planes via an esterification reaction, with benzoic acid functionalities pre-attached on graphene with its aryl diazonium salt precursor. AFM and SEM analysis revealed the successful preparation of single layered graphene sheets and graphene/polymer hydrogels with pH controlled porous structures. Attenuated total reflection infrared (ATR-IR) and thermogravimetric analyzer (TGA) verified the successful stepwise preparation of graphene/PAA hydrogel. This graphene/PAA hydrogel was pH-sensitive and more mechanically elastic than the PAA hydrogel prepared without graphene. The pH sensitivity of the hydrogel was further utilized for controlled drug release. Doxorubicin was chosen as a model drug and loaded into the hydrogels. The drug loading and release experiment indicated that this hydrogel can be used to efficiently control drug release in the intestine environment (pH = 7.4), better than release in a more acidic environment.
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FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Ye Zi,Cuiping Shi,Guangyi Kan,Jiawei Peng,Huan Gong,Xichang Wang,Jian Zhong
The development of genipin modification methods has attracted much attention to improve the functional properties of proteins in the fields of food science . In this work, two types of pH-responsive genipin-crosslinked bovine bone gelatin conjugates with high surface hydrophobicity were developed for emulsion stabilization. One was genipin molecule-crosslinked gelatin conjugates prepared at acidic pH and the other was genipin macromer-crosslinked gelatin conjugates prepared at neutral and basic pH. The degrees of crosslinking increased with the increase of preparation pH. They increased with the increase of genipin-to-gelatin mass ratios at acidic pH, whereas decreased with the increase of genipin-to-gelatin mass ratios at neutral and basic pH. The surface hydrophobicity values, molecular weights, and emulsifying properties (creaming stability, emulsion activity index, and emulsion stability index) of the obtained conjugates increased with the increase of both preparation pH and genipin-to-gelatin mass ratios. This work provided useful knowledge to understand how the preparation parameters (preparation pH and genipin-to-gelatin mass ratio) and genipin structures (molecule and macromer) affect a protein's degrees of crosslinking, physicochemical properties, and emulsifying properties.
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Journal of Materials Chemistry A
·
2024
·
IF 10.7
Ben Zhang,Yijuan Zheng,Zhenyu Xing,Zihe Wu,Chong Cheng,Tian Ma,Shuang Li
Designing and synthesizing eco-friendly and efficient catalysts for the hydrogen evolution reaction (HER) plays a crucial role in the field of large-scale and high-purity hydrogen production. However, it is severely hampered by the sluggish kinetics of the water dissociation step in non-acidic media. To optimize catalytic kinetics, interfacial electron engineering between active sites and supports has been regarded as an essential strategy to achieve compelling electrocatalytic all-pH-value-hydrogen production. Herein, we present the successful fabrication of WNO-coupled Rh layers (Rh–WNO) by a simple thermal treatment of a urea sol–gel with mixed W and Rh salts. This facile one-pot synthetic method is easy to scale up and the resulting Rh–WNO catalyst features Rh nanoclusters anchored on the surface of the well-ordered WNO crystals via Rh–N and Rh–O bonds. The optimal catalyst, Rh–WNO, delivered low overpotentials of 19, 22, and 134 mV at a current density of 10 mA cm−2 in 0.5 M H2SO4, 1.0 M KOH, and 1.0 M PBS, respectively. Notably, Rh–WNO exhibits a high turnover frequency (TOF) of 7.84 H2 s−1 in alkaline conditions, which is much higher than those of Pt/C (0.9 H2 s−1), Rh/C (0.77 H2 s−1), and most of the reported HER electrocatalysts. This work may provide an effective and economic approach for scaling up the production of highly efficient and stable metal nitride-based HER catalysts.
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Journal of Tissue Engineering
·
2024
·
IF 6.7
Yue Zhao,Yinlian Yao,Shilong Fan,Xin Shen,Xingxing Chai,Zimin Li,Jiachun Zeng,Jiang Pi,Zhikun Zhou,Gonghua Huang,Hua Jin
The incidence of ulcerative colitis (UC) is rapidly rising worldwide. Oral drug delivery system is a promising approach for treating UC, but it often fails to accumulate to the inflammatory lesions, thus, it is impressive to develop a colon-targeted oral delivery system for preventing systemic toxicity and maintaining UC therapeutics. Here, a negative-charged PLGA nanoparticle system was designed to encapsulate celastrol (Cel), and then chitosan and mannose were coated on the surface of the nanoparticles (MC@Cel-NPs) to endow these nanoparticles with the mucosal adsorption and macrophage targeting abilities. MC@Cel-NPs demonstrate excellent resist decomposition ability against the strong acidic gastrointestinal environment, and accumulates in the specific inflammatory sites through the affinity of electrostatic reaction. After releasing the payload, MC@Cel-NPs could remarkably alleviate the colon inflammation, which was evidenced by the decrease in pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 in both blood and colon sections, and scavenging reactive oxygen species (ROS) in colon cells, including macrophage, neutrophil, T cell, and B cell. This nanoparticle system provided a new approach for treating UC through a Chinese herbal ingredient-related oral delivery manner.
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