Nanomaterials
·
2024
·Vol. 14
·期 10
·838
·
IF 4.4
Hangyu Yan,Guowei Li,Fengyuan Zhang,Jingsong Liu,Mengdai Luoshan
Noble metal nanomaterials with a localized surface plasmon resonance effect exhibit outstanding advantages in areas such as photothermal therapy and photocatalysis. As a unique plasmonic metal nanostructure, gold nanobipyramids have been attracting much interest due to their strong specific local electric field intensity, large optical cross sections, and high refractive index sensitivity. In this study, we propose a novel three-component hetero-nanostructure composed of rough gold nanobipyramids (R-Au NBPs), Pt, and CdS. Initially, purified gold nanobipyramids are regrown to form R-Au NBPs that have a certain degree of roughness. These R-Au NBP substrates with a rough surface provide more hotspots and strengthen the intensity of localized electric fields. Subsequently, Pt and CdS nanoparticles are selectively deposited onto the surface of R-Au NBPs. Pt nanoparticles can provide more active sites. Each component of this hetero-nanostructure directly contacts others, creating multiple electron transfer channels. This novel design allows for tunable localized plasmon resonance wavelengths ranging from the visible to near-infrared regions. These factors contribute to the final superior photothermal conversion performance of the R-Au/Pt-CdS nanohybrids. Under the irradiation of near-infrared light (1064 nm), the photothermal conversion efficiency of R-Au/Pt-CdS reached 38.88%, which is 4.49, 1.5, and 1.22 times higher than that of Au NBPs, R-Au NBPs, and R-Au NBPs/Pt, respectively.
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Pharmaceuticals
·
2025
·Vol. 18
·期 7
·1079
·
IF 4.8
Rui Wang,Yuqian Yang,Tong Zhou,Bingjie Zou,Li Ding
Objectives: (R)-ketamine hydrochloride injection is a novel, rapid-acting antidepressant for the treatment of treatment-resistant depression. The aim of this study was to assess the pharmacokinetics, safety, and tolerability of (R)-ketamine hydrochloride injection in healthy Chinese subjects following ascending single intravenous doses ranging from 10.0 mg to 180 mg.Methods: This randomized, double-blind, placebo-controlled study was conducted in 50 healthy male and female Chinese subjects after single ascending doses of (R)-ketamine hydrochloride injection (10.0, 30.0, 60.0, 120, and 180 mg). Ten subjects (including two subjects treated with a placebo) were included in each dose cohort. Pharmacokinetic characteristics, safety, and tolerability profiles of the study drug were evaluated.Results:After the intravenous doses administered from 10.0 mg to 180 mg of (R)-ketamine hydrochloride injection to the subjects, the Cmaxand AUC values for both (R)-ketamine and its metabolite (R)-norketamine in the subjects increased approximately proportionally to the doses. The average peak plasma concentration levels at the five dose cohorts ranged from 56.0 to 1424 ng/mL and 27.7 to 491 ng/mL for (R)-ketamine and (R)-norketamine, respectively. The adverse events of (R)-ketamine hydrochloride injection were temporary and recovered spontaneously without treatment.Conclusions: In summary, (R)-ketamine hydrochloride injection was safe and well tolerated in healthy Chinese subjects. The clinical study results laid a foundation for the further clinical studies of (R)-ketamine hydrochloride injection in patients.
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WATER AIR AND SOIL POLLUTION
·
2025
·Vol. 236
·期 10
·1-14
·
IF 3
Liu Jingliang,An Fengxia,Zheng Di,Li Chao,Sun Jingya
In this study, Mn-Ni-layered double hydroxides (Mn-Ni-LDHs) were synthesized and utilized to activate peroxymonosulfate (PMS) for degrading reactive brilliant blue KN-R (KN-R) in aqueous solutions. The characterization results demonstrate that the catalysts synthesized are layered structures, and the surface area is 169.3 m 2 /g. The Mn-Ni-LDHs exhibited significant efficiency effective for the degradation of KN-R via activating PMS, achieving 96.5% degradation of KN-R within 90 min under optimal conditions (0.20 g/L catalyst, 10 mM PMS, pH=10.0 and 25°C). The reaction followed pseudo-first-order kinetics with a rate constant of 0.0351 min -1 , and the apparent activation energy was calculated to be 11.94 kJ/mol. Electron paramagnetic resonance (EPR) and free radical scavenging experiments identified ·O 2 − and 1 O 2 as the primary reactive species involved in the reaction for KN-R degradation. The proposed catalytic mechanism suggests that redox cycling between Mn 4+ /Mn 3+ and Ni 3+ /Ni 2+ within Mn-Ni-LDHs plays a critical role in enhancing PMS activation, thereby promoting the continuous generation of active oxygen species.
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ISME Journal
·
2025
·
IF 10
Yan Baofei,Zheng Xian,Lu Danya,Li Ting,Chen Xi,Shao Zhitao,Fu Tingming
Microbiota-associated factors are increasingly recognized as significant contributors to the progression of ulcerative colitis, and microbial modulation has emerged as an effective therapy for this condition. The herbal compound silibinin has demonstrated properties that modulate gut microbiota. Herein, we investigated the response of gut microbiota to silibinin in ameliorating colitis, using a mouse model of colitis coupled with antibiotic exposure. Results indicated that antibiotic pretreatment negated the benefits of silibinin in mice with colitis. Furthermore, fecal microbiota transplantation involving silibinin-modulated gut microbiota further substantiated the gut microbiota-dependent effects of silibinin. Within the metabolic profiles of silibinin-regulated microbiota, we identified that Alistipes-associated (R)-2,3-dihydroxy-isovalerate exhibited the most pronounced anti-inflammatory effects in vitro and demonstrated protective effects against colitis. Moreover, (R)-2,3-dihydroxy-isovalerate reinstated the protective effects of silibinin in mice with colitis under antibiotic exposure. These effects were primarily mediated via the targeting of the colonic GABA transporter 3 by (R)-2,3-dihydroxy-isovalerate. We further revealed that the retinoic acid receptor β and the retinoid-related orphan nuclear receptor γt may mediate the impact of silibinin-derived microbiota and (R)-2,3-dihydroxy-isovalerate on colitis. Additionally, the knockdown of colonic GABA transporter 3 diminished the impact of silibinin on the GABA transporter 3/retinoic acid receptor β/retinoid-related orphan nuclear receptor γt axis and colitis. Our findings highlight that (R)-2,3-dihydroxy-isovalerate, enriched from microbiota derived from silibinin, can target the GABA transporter 3/retinoic acid receptor β/retinoid-related orphan nuclear receptor γt axis, which is essential for anti-colitis properties of silibinin-regulated microbiota.
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Frontiers in Pharmacology
·
2022
·
IF 5.988
Dong Zhen,Tang Shu-sheng,Ma Xiao-lan,Tan Bin,Tang Zhao-shan,Li Chang-hong,Yang Zi-hui,Zeng Jian-guo
The protopine alkaloids are widely distributed within the opium poppy family and have a wide range of pharmacological effects. MPTA is a product of the protopine total alkaloids extracted from the Macleaya cordata (Willd.) R. Br. Previously, we reported good anti-inflammatory activity of MPTA as well as oral acute and sub-chronic toxicity studies in rats. In order to perform a systematic toxicological safety assessment of MPTA, oral acute toxicity, genotoxicity (bone marrow cell chromosome aberration test, sperm abnormality test, bone marrow cell micronucleus test, and rat teratogenicity test), and chronic toxicity in mice were performed in this study. In the oral acute toxicity test, the LD50 in ICR mice was 481.99 mg/kg, with 95% confidence limits ranging from 404.27 to 574.70 mg/kg. All three mutagenicity tests tested negative in the range of 60.25–241.00 mg/kg. The results of the teratogenicity test in rats showed no reproductive or embryonic developmental toxicity at only 7.53 mg/kg, which can be considered as a no observed effect level (NOEL) for the teratogenicity test. Therefore, MPTA is safe for use at the doses tested, but attention should be paid to the potential risk to pregnant animals and the safety evaluation and toxicity mechanisms in target animals should be further investigated.
研究领域:
毒理学
·
药理学
·
遗传学
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Synthetic and Systems Biotechnology
·
2025
·
IF 4.4
Ruizhou Tang,Jiahuan Li,Xiaole Yang,Xia Tian,Zehui Wang,Xuning Zhang,Tingting Li
Aminotransferases are promising green biocatalysts for the synthesis of chiral amines, yet their limited catalytic efficiencies restrict broader industrial applications. In this study, a novel ( R )-amine transaminase, FalAT, was identified from Fusarium albosuccineum through genome mining. FalAT exhibited optimal activity at 30 °C and pH 7.0 and catalyzed the conversion of 1-Boc-3-piperidone to ( R )-1-Boc-3-aminopiperidine with >99 % enantiomeric excess. To efficiently enhance its catalytic performance, a Substrate–Protein Interaction Network (SPIN) strategy was implemented, integrating structure-guided analysis, molecular docking, virtual saturation mutagenesis, and dual energy–distance filtering. SPIN first screened and constructed a mutational library covering 70 amino acid positions, which was subsequently narrowed to 9 key residues, ultimately yielding 15 candidate mutants for experimental validation. Experimental results showed that five mutants exhibited higher catalytic activity than the wild-type enzyme, among which R126A was the most effective, displaying approximately a 4-fold increase in catalytic activity and a 13-fold enhancement in catalytic efficiency ( k ca t /K m = 2.05 s −1 mM −1 ). Molecular dynamics simulations revealed that the R126A mutation expanded the active-site cavity, alleviated steric hindrance, and strengthened hydrophobic interactions, thereby improving substrate binding and catalytic turnover. Furthermore, substrate profiling demonstrated that FalAT possesses moderate substrate promiscuity. Overall, the SPIN strategy significantly improved the catalytic performance of FalAT while markedly reducing experimental workload, providing an efficient and generalizable approach for the directed evolution of ( R )-amine transaminases for the green synthesis of chiral amines.
研究领域:
酶工程
·
合成生物学
·
生物催化
·
结构生物学
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分子设计
·
绿色化学
·
药物代谢
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Materials Today Communications
·
2021
·
IF 3.383
Bushra Naureen,A.S.M.A. Haseeb,W.J. Basirun,Farina Muhamad
Tissue degeneration due to diseases, accidents, and wars is a worldwide issue. One of the methods used by regenerative medicine (RM) to aid tissue repair is by using a biodegradable tissue engineering scaffold (TES). Different body tissues have variable regeneration abilities, and hence, they require TES with variable degradation rates. This paper focuses on synthesising four polyurethane foam scaffolds (PUFS) with variable degradation rates based on the concentration of incorporated chain extender (CE) poly[(R)-3-hydroxybutyrate] (P3HB). The polyol employed is poly (propylene fumarate) (PPF) along with aliphatic 1,6-hexamethylene diisocyanate (HDI). GPC provided the molecular weight (M n = 455.67 g mol −1 , M w = 587.15 g mol −1 ) and PDI (1.29) of the PPF. FTIR and 1 H and 13 C NMR confirmed the proposed structures (C C, COOR, and NHCOO) of the PUFS. The FESEM images revealed a bimodal pore structure , with random, irregular, interconnected pores (242–340 µm) and discrete void spaces (735–860 µm). The size of PUFS pores and void spaces (PU4 > PU3 > PU2 > PU1) is directly proportional to their respective degradation rates. The TGA/DTA curves show a decrease of an onset thermal decomposition temperature from PU1 to PU4 (267.17 ºC PU1, 267.28 ºC PU2, 256.01 ºC PU3 and 240.72 ºC PU4) due to the respective increase in CE concentration. The results of in-vitro degradation analysis at day 70, revealed significant difference between the weight loss (%) of each group (PU1 = 4.09 ± 0.18%, PU2 = 8.03 ± 0.17%, PU3 = 13.14 ± 0.12% and PU4 = 20.21 ± 0.19%). Thus, PUFS with a higher concentration of P3HB have a faster degradation rate due to the associated P3HB hydrolysable ester/-COOR functional group within the PUFS backbone. The chemical structure , bimodal morphology, and variable degradation rate of these PUFS could be employed for various RM applications.
研究领域:
生物材料学
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再生医学
·
高分子化学
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ENZYME AND MICROBIAL TECHNOLOGY
·
2012
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IF 2.367
Jing-Yun Wang,Cui-Li Ma,Yong-Ming Bao,Pin-San Xu
Lipase from Burkholderia cepacia was encapsulated inside zirconia particles by biomimetic mineralization of K 2 ZrF 6 induced with protamine, a natural cationic protein. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FT-IR) were employed for the characterization of the novel immobilized lipase. SEM and TEM images showed that both the zirconia particles with and without lipase have good spherical structures with average particle sizes of 150 nm. Fluorescence microscopy demonstrated that the lipase was indeed encapsulated inside the zirconia particles. The maximum immobilization capacity of the zirconia particles was 0.15 units/mg under optimum immobilization conditions. Biochemical characterization showed that the encapsulated lipase could retain most of its initial activity. Compared with free lipase, the encapsulated lipase exhibited improved thermal, pH, and recycling stabilities. After 8 weeks of storage, no substantial loss in catalytic activity was observed for the encapsulated lipase. The conversion of the kinetic resolution of ( R,S )-1-phenylethanol with vinyl acetate as acetyl donor catalyzed by zirconia-immobilized lipase reached 49.9% with higher ee s of 99.9% under the following optimal conditions: octane as solvent, 0.1 M ( R,S )-1-phenylethanol, 70 mg immobilized lipase, 180 rpm, 50 °C for 48 h. After 6 cycles (288 h), the conversion and ee s were still 43% and 85%, respectively.
研究领域:
生物化学
·
材料科学
·
酶工程
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JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Hongyun Niu,Siyu Zhu,Xiaoling Gu,Shaodong Jiang,Yaqi Cai
The extensive use of sulfonamides (SAs) in the treatment of respiratory and bacterial infections for humans and animals have triggered their ubiquitous occurrence in environment. Anodic oxidation processes have prominent potential in degrading SAs from wastewater. Herein, carbon cotton (CC) and graphite plate (GP) are applied as anodes to degrade SAs with various R substitutions at low current density (j, 0.42–5 mA/cm2, current intensity 2.5–30 mA). At j = 1.67 mA/cm 2 , degradation efficiency of sulfathiazole, sulfadiazine, sulfisoxazole, sulfamethoxazole, sulfapyridine, and sulfamethazine reached 100, 90.1, 81.6, 92.8, 84.2, and 90.8 % at CC, and 100, 81.5, 54.2, 79.3, 83.2 and 79.1 % at GP, respectively. Most of the degraded SAs have been mineralized in both reaction systems. Sulfaguanidine, without heterocycle in R substituent, was more refractory to degrade at CC and GP surface even at higher j. The quenching experiments and DFT calculation disclosed that direct electron transfer (DET) process took a fundamental role on SAs decomposition. The surface sorbed [•OH] was responsible for mineralization of SAs. The two anodes can efficiently degraded sulfathiazole in different high-salinity wastewater samples at low j. Low-energy consumption and excellent mineralization efficiency endowed CC and GP with promising application for electrochemical oxidation of SAs from wastewater.
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CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Bohan Liu,Yihui Zou,Shuai Chen,Huawei Zhang,Jin Sun,Xilin She,Dongjiang Yang
Due to the high theoretical capacity and open frameworks, the low-cost sodium iron pyrophosphate Na 3.12 Fe 2.44 (P 2 O 7 ) 2 has become a promising candidate for cathode materials of the sodium ion batteries (SIBs). However, NFPO is very sensitive to moisture and CO 2 , which causes serious surface oxidation reaction and results in degraded high-rate charge/discharge performance and cyclic stability. Herein, we developed a seaweed-derived synthesis strategy to prepare air stable NFPO/r-GO aerogels by using sodium alginate (SA) and reduced graphene oxide (r-GO) as precursors. X-ray photoelectron spectroscopy (XPS) proved that there were no residual Na species on the surface of prepared NFPO/r-GO aerogels and no any oxidized impurities after exposing in air for 100 days. When evaluating the NFPO/r-GO aerogels as cathode materials for SIBs, the sample exhibits excellent reversible capacity of 116.1 mAh g −1 at 0.1 C, 88.82% capacity retention after 5000 cycles and superior rate capacity of 72.3 mAh g −1 at 20 C. The superior electrochemical performances are mainly attributed the delicate structure, which can not only increase the electronic conductivity, but also improve air stability of NFPO by suppressing surface side reactions with moisture and CO 2 .
研究领域:
材料科学
·
电化学
·
能源存储
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DALTON TRANSACTIONS
·
2026
·
IF 3.3
Cai-Ming Liu,Yi-Quan Zhang,Xiang Hao,Xi-Li Li
It is still a major challenge to construct multifunctional complexes using axial chiral ligands. Herein, the axial chiral ligands (R)/(S)-1,1'-binaphthyl-2,2'-diyl hydrogen phosphate (R-HL/S-HL) was used as a bridging ligand, and together with the Schiff base bridging ligand {H2LSchiff = [2-[(E)-(5-fluoro-2-hydroxyphenyl)methylidene]diazanyl](4-chloropyridin-2-yl)methanone}, two pairs of homochiral Dy2 complexes [Dy2(R-L/S-L)2(LSchiff)2(DMA)2]·3DMA·4H2O (R-1/S-1) and [Dy2(R-L/S-L)2(LSchiff)2(DMF)2]·3DMF (R-2/S-2) were successfully constructed at room temperature in different solvents (DMA vs DMF). Interestingly, although their coordination modes as terminal ligands are very similar, the subtle differences between DMA and DMF molecules cause changes in the space groups: R-1 and S-1 crystallize in the P1 chiral space group whereas R-2 and S-2 in the P212121 chiral space group, which may affect their nonlinear optical properties: R-1 and S-1 exhibits both SHG and THG responses, while R-2 and S-2 only displays a THG response. Notably, R-1/S-1 and R-2/S-2 are ferromagnetic coupling, and behave as single-molecule magnets (SMMs) at 0 Oe, and their magnetic properties were further studied using Ab initio calculations. Moreover, the magneto-optical effects of R-1/S-1 and R-2/S-2 were investigated by their magnetic circular dichroism (MCD) spectra.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Bin Yan,Hongru Zhao,Bingmei Su,Juan Lin
Protein engineering altered the reaction direction of TsRTA for AP. • Adjusting the catalytic center and entrance of TsRTA promoted AP binding. • A 10-site variant of TsRTA enabled biosynthesis of ( R )-1-PEA. • An acetone elimination module increased Mu10 productivity by 57.4%. ( R )-1-Phenylethylamine [( R )-1-PEA] is an important chiral compound widely used in pharmaceuticals, chemical synthesis, and materials science. ω-Transaminases are valuable biocatalysts for the asymmetric amination of ketone substrates under mild conditions and represent an attractive tool for the green synthesis of chiral amines. However, in natural ( R )-selective transaminases, acetophenone (AP) is more commonly produced as the deamination product of ( R )-1-PEA, whereas its amination as an amino acceptor is generally inefficient. In this study, an ( R )-selective ω-transaminase from Thermomyces stellatus (TsRTA), which originally catalyzed only the deamination of ( R )-1-PEA to AP, was selected as the engineering target. Protein engineering was performed to shift its reaction equilibrium, thereby enabling the asymmetric synthesis of ( R )-1-PEA from AP. To overcome the thermodynamic limitation caused by acetone accumulation, an “acetone elimination–cofactor regeneration” coupled module was introduced to improve the whole-cell catalytic efficiency for ( R )-1-PEA synthesis using AP as the substrate. Under optimal reaction conditions (35 °C, pH 10.0, 0.5 mM PLP, an isopropylamine/AP molar ratio of 4:1, 200 mM formate, and 0.2 mM NAD + ), the whole-cell catalytic system produced 67 mM ( R )-1-PEA from 100 mM AP within 8 h. This study lays a foundation for the scalable preparation of ( R )-1-PEA and provides a useful reference for regulating the reaction direction of transaminases. Download: Download high-res image (205KB) Download: Download full-size image
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