LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
·
IF 4.952
Pengfei Zhou,Daobang Tang,Jinhao Zou,Xuping Wang
The low stability and loss of antimicrobial activity of catechins limited their application in food preservation. In this study, four kinds of catechins including (−)-epicatechin (EC), (−)-epigallocatechin (EGC), (−)-epicatechin-3-gallate (ECG), and (−)-epigallocatechin-3-gallate (EGCG) were selected as model compounds. Natural deep eutectic solvents (NADES) are a new generation of green solvents that have attracted much attention due to their unique characteristics. The effects of NADES on the stability and antimicrobial activity of catechins were investigated in the present study. Eight typical NADES, especially choline chloride/glycerol (ChG), were found to significantly enhance the thermal and storage stability of catechins. This finding accounted for by hydrogen bonding network formation between ChG and catechins, confirmed by FT-IR and 2D NMR analysis. Additionally, ChG decreased the minimum inhibitory concentration of catechins on Gram-positive and Gram-negative bacteria, which may be associated with its stabilizing property. The stability of catechins combined with NADES revealed their potential of applications in the food industry.
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Journal of Functional Foods
·
2021
·
IF 4.451
Xiaolan Chen,Bingyan Cai,Jing Wang,Zhicun Sheng,Haifeng Yang,Dada Wang,Jiahao Chen,Qingqing Ning
Mulberry leaf polysaccharide (MLP) possess many biological characteristics, such as immunomodulatory and antioxidant properties, but whether MLP can impact the distribution and characteristics of the gut microbiota is not yet clear. In this study, the effects of MLP on diversity- and immune-related factors and short-chain fatty acids (SCFAs) of the gut microbiota in mice were investigated. The results showed that MLP could restore the indexes of the thymus and spleen, the damaged intestinal barrier and levels of inflammatory cytokines in cyclophosphamide (CTX)-treated mice, and alleviate oxidative damage to the liver. Additionally, MLP were associated with a relatively higher abundance of Bacteroidetes but lower levels of Firmicutes, Butyricimonas and Eubacterium in CTX-treated mice. Moreover, MLP restored the levels of acetic acid, propionic acid, and n-butyric acid reduced by CTX. These results indicated that MLP, as a special ingredient, might regulate the immune response by altering the spatial structure of the gut microbiota and the production of SCFAs.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Xiao-Guo Ji,Kun-Lin Chang,Mai Chen,Liang-Liang Zhu,Arwa Osman,Hao Yin,Li-Ming Zhao
Chitooligosaccharides (COS), with a specific degree of polymerization, were evaluated for their effect on human fecal microbiota, as well as their metabolite, using an in vitro anaerobic batch fermentation model. COS increased the richness and diversity of the gut microbial community. More importantly, the microbial community structure of the COS groups was significantly different from the control and fructooligosaccharides (FOS) groups. COS specifically enriched the butyric and propionic acid-producing microbiota and targeted Faecalibacterium , Clostridium_sensu_stricto_1 , C._sensu_stricto_13 , and Fusicatenibacter . Furthermore, COS increased the acetic, propionic, and butyric acid contents. The production of propionic acid was greater than that obtained using FOS. Metabolome analysis suggested that COS could upregulate allocholic acid, 1,3-butanediol, 3,4-dihydroxybutanotic acid, and 2-hydroxybutyric acid production and downregulate putrescine, ethanamine, hexanoic acid, and branched chain amino acid ( l -valine and l -leucine) production in the fermentation broth. These differential metabolites were closely related to lipid, glucose, and amino acid metabolism. These results should inform further development of COS as potential raw materials for functional foods and health products.
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CARBOHYDRATE POLYMERS
·
2021
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IF 9.381
Feng Shen,Hao Ling,Wenjiao Ge,Yang Yang,Xiaoying Wang,Junli Ren,Xiaohui Wang
In this work, a series of amphiphilic hemicellulose-based grafting polymers are synthesized by homogeneous esterification with various hydrophobic chain fatty acids. With the flexible chain conformation of hemicelluloses, the hemicellulose-graft-fatty acid is self-assembled into hard sphere micelles in aqueous solution through hydrophobic interactions. The resultant micelles show apparent hydrodynamic radius (R h ) varying in the range of 34–57 nm and radius of gyration (R g) varying from 30 to 44 nm. Moreover, the R h , R g , zeta potential and critical micelle concentration (CMC) of the micelles gradually decreases with the increasing hydrophobic chain length. The aggregation number of micelles can be tuned by varying alkyl chain lengths. Taking curcumin (Cur) as a model drug, the hemicellulose micelles were investigated as drug carriers, which exhibit a chain length-controlled drug release behavior. Taken together, structure and property tunable hemicellulose-based micelles were obtained, and showed potential of application in pharmaceutical, food, and cosmetics fields.
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PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2021
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IF 4.384
Yu Cai,Qi Tian,Chao Liu,Liang Fang
The purpose of present study was to develop a long-acting drug-in-adhesive patch of rivastigmine (RVS) to achieve controlled release under high drug loading. Formulation factors including ion-pair, pressure sensitive adhesive (PSA), drug-loading and permeation enhancers were investigated through in vitro skin permeation experiments. Optimized patch was evaluated by pharmacokinetic study. The mechanism of controlled release was studied by FTIR, Raman, DSC , rheology study and molecular modeling . The optimized patch composed of RVS-SA (equal to 30% RVS), 15% POCC as permeation enhancer and AAOH as PSA matrix. The RVS in optimized patch was basically permeated at a uniform rate, and the ratio of the skin permeation amount (2803.38 ± 153.85 μg/cm 2 ) in 72 hours to that of the control group (1000.89 ± 62.45 μg/cm 2 ) was 2.8. The plasma concentration of RVS was stable for 72 hours in vivo ( AUC optimized = 5721.30 ± 1994.87 h ng/mL, MRT 0-t = 29.55 ± 2.49 h), and C max was significantly controlled. The results of the study on the controlled release mechanism showed that the addition of counter ion formed hydrogen bonds with RVS and PSA respectively, which reduced the fluidity and molecular mobility of PSA, and enhanced the interaction between RVS and PSA, thus achieving the purpose of long-acting effect. In conclusion, long-acting drug-in-adhesive patch of RVS was developed, and provided a new idea for the long term drug delivery of Alzheimer's disease .
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Food Frontiers
·
2021
·Vol. 2
·期 2
·208-217
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IF 0
Huiting Lin,Jiawen Zhang,Shiyang Li,Baodong Zheng,Jiamiao Hu
The current study was aimed to explore the beneficial effects of p olysaccharides isolated from Laminaria japonica (LP) on gestational diabetes mellitus (GDM) mice. The obtained results demonstrated that the LP improved serum biochemical index, body weight index, and glucose tolerance. Furthermore, the evidence also suggested that the beneficial effects of LP might be attributed to the alternation in gut microbiota by LP supplementation. Particularly, the Turicibacter , a short-chain fatty acids-producing bacterium, was found to be up-regulated. In conclusion, the obtained results indicated that the LP might serve as prebiotic, and might have a great potential to be used as food supplementation for GDM patients.
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Food & Function
·
2021
·Vol. 12
·期 12
·5524-5538
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IF 5.396
Bo Li,Xinglishang He,Hai-Ying Jin,Hui-Ying Wang,Fu-Chen Zhou,Ning-Yu Zhang,Dong-Ying Jie,Lin-Zi Li,Jie Su,Xiang Zheng,Rong Luo,Gui-Yuan Lv,Ning-Hua Jiang,Qiao-Xian Yu,Su-Hong Chen
Given the increasing global trend toward unhealthy lifestyles and dietary decisions, such as “over-consumption of alcohol, and high sugar and fat diets” (ACHSFDs), it is not surprising that metabolic hypertension (MH) is now the most common type of hypertension. There is an urgent, global need for effective measures for the prevention and treatment of MH. Improper diet leads to decreased short-chain fatty acid (SCFA) production in the gut, leading to decreased gastrointestinal function, metabolism, and blood pressure as a result of signaling through G-protein-coupled receptors (GPCRs), ultimately causing MH. Previous studies have suggested that Dendrobium officinale (DO) may improve gastrointestinal function, lower blood pressure, and regulate metabolic abnormalities, but it is not clear whether it acts on MH by increasing SCFA and, if so, how. In this research, it was observed that Dendrobium officinale ultrafine powder (DOFP) could lower blood pressure and improve lipid abnormalities in ACHSFD-induced MH model rats. Moreover, DOFP was found to improve the intestinal flora and increased the SCFA level in feces and serum, as well as increased the expressions of GPCR43/41 and eNOS and the nitric oxide (NO) level. An experiment on isolated aorta rings revealed that DOFP improved the vascular endothelial relaxation function in MH rats, and this effect could be blocked by the eNOS inhibitor L-NAME. These experimental results suggest that DOFP improved the intestinal flora and increased the production, transportation, and utilization of SCFA, activated the intestinal-vascular axis SCFA-GPCR43/41 pathway, improved vascular endothelial function, and finally lowered blood pressure in MH model rats. This research provides a new focus for the mechanism of the effect of DOFP against MH by triggering the enteric-origin SCFA-GPCR43/41 pathway.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Junjun Li,Han Jin,Ximei Yan,Dongyan Shao,Xinzhong Hu,Junling Shi
Artemisia sphaerocephala Krasch polysaccharide (ASKP) consists of two main fractions, 60P (molecular weight at 551 kDa) and 60S (molecular weight at 39 kDa). The anti-obesity effects of ASKP and its two fractions were investigated in high-fat-diet-fed mice and showed similar capability in efficiently preventing the development of obesity. The final body weight and body weight gain of obesity mice model were reduced by 12.44% and 35.33% by ASKP, 10.63% and 34.35% by 60P, and 7.82% and 20.04% by 60S. They also showed similar efficiency to ameliorate dyslipidemia, systematic inflammation, and gut dysbiosis. The colonic genes of barrier integrity were significantly upregulated and the genes of hepatic lipid metabolism and that of colonic inflammatory response were suppressed. They attenuated the gut dysbiosis in obese mice, such as the significant enrichment of beneficial genera ( Bifidobacterium and Olsenella ) and suppression of harmful ones ( Mucispirillum and Helicobacter ). Significant enrichment of carbohydrate metabolism associated with the promotion of short-chain fatty acid production and decrease of the metabolisms related to obesity and gut dysbiosis (valine, leucine, and isoleucine biosynthesis, and nitrogen metabolism) were also observed by the administration of ASKP, 60P, and 60S. Overall, these polysaccharides showed potential in acting as prebiotics in preventing high-fat-diet-induced obesity.
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Journal of Functional Foods
·
2021
·
IF 4.451
Guoliang Wang,Diying Zhong,Haohan Liu,Tao Yang,Qiqi Liang,Jing Wang,Runguang Zhang,Youlin Zhang
Dietary fiber intake is associated with a low incidence of suffering from metabolic syndrome. To explore the potential health benefits of walnut meal dietary fiber (WMDF) as a prebiotic, the functional role of WMDF on metabolic syndrome in mice induced by high fructose diet (20%, HF) was investigated. The animal experiment results showed that administration of WMDF to HF-fed mice alleviated abnormal body weight gain, insulin resistance, oxidative stress, lipid metabolism disorders and inflammation. Histopathological observation confirmed the preventative effects of WMDF on hepatic steatosis and vascular endothelial dysfunction. Also, WMDF intake increased the production of acetic acid, propionic acid and butyric acid. Moreover, WMDF ingestion effectively improved the disorder of gut microbiota caused by HF, increased the diversity of gut microbiota and the relative abundance of short-chain fatty acids-producing bacteria. These findings demonstrate WMDF can be used as a prebiotic to prevent HF-induced metabolic syndrome.
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TALANTA
·
2021
·
IF 6.057
Hua-Ming Xiao,Yan Shi,Yong-Xue Yang,Shuai Zhao,Xian Wang
The free fatty acids that contain one to eight carbons (C 1 –C 8 ) in biodiesel would affect the quality of biodiesel. It is still a matter of challenge to simultaneously determine the composition of C 1 –C 8 fatty acids in seed oil and seed oil-based biodiesel. Herein, a novel method of charge derivatization coupling with direct infusion mass spectrometry (CD-DIMS) was developed for the determination of the C 1 –C 8 fatty acids in biodiesels. A fixed-charge derivatization reagent, 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide (CMCT), was used to convert fatty acids into their cationic derivatives, which significantly improved the sensitivity and selectivity of detection. Good linearity was observed with the limits of detection (LODs) in the range of 0.0002–0.001 μg mL −1 for the investigated fatty acids. The recovery was in the range of 85.1%–101.9% and the matrix effect was within the range of 75.5–93.2%. The developed method was carried out to analyze C 1 –C 8 fatty acids in rubber seed oil (RSO) and RSO-based biodiesels produced by different catalysts, including NaOH, TiO 2 , and carbodiimide . It was also applied to the dynamic monitoring of C 1 –C 8 fatty acids in RSO and produced RSO biodiesels during the oxidation process. As results, formic acid , acetic acid, and propionic acid were detected in aged RSO and biodiesel samples. The contents of formic acid, acetic acid, and propionic acid all increased in aged RSO and biodiesels, but with different growth rates. These results demonstrated that the developed CD-DIMS method can provide a quick, accurate, and sensitive analysis of C 1 –C 8 fatty acids in seed oil and biodiesel samples.
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FOOD AND AGRICULTURAL IMMUNOLOGY
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2021
·
IF 3.101
Jie-Biao Guo,Tai-Long Wei,Qing-Hua He,Jin-Sheng Cheng,Xiu-Zhen Qiu,Wang-Pei Liu,Xian-Quan Lan,Lu-Fen Chen,Min Guo
A magnetic separation-based immunosensor for deoxynivalenol assay, coupled with a nano-affinity cleaning up for instrumental confirmation, was developed. Maltose-binding protein with mimic epitope of DON was coupled to CdSe/ZnS quantum dot nanobeads for fluorescent reporter. Graphene oxide was linked with magnetic nanoparticles and protein G for immunomagnetic adsorbent. The magnetic separation of reporter could be inhibited by DON so the fluorescent remained in the solution indicates a good quantitative correlation to DON concentration. The DON separated by immune adsorbent can be cleaned up for instrumental confirmation. The optimized immunosensor showed results as follows: the linear range was in 0.3–3000 ng mL−1. The limits of detection (S/N = 3) were 0.50, 1.0, and 3.0 ng mL−1 in different backgrounds. The recoveries for DON-spiked samples range from 78.7% to 88.5%. Both results of UPLC-MS/MS based on affinity column purification and coupled with nano-affinity cleaning up showed a good agreement with the current immunosensor.
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ACS Omega
·
2020
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IF 2.87
Yumeng Yang,Dan Gao,Rui Qian,Yuyang Jiang
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using conventional organic matrices for detection of small molecules has some limitations, such as heterogeneous analyte/matrix co-crystals, as well as interference of matrices in the low-molecular-weight range. In this work, a zeolite framework nanomaterial, TS-1, was applied as a MALDI matrix for the analysis of small molecules by MALDI-MS for the first time. To improve the signal intensity and reproducibility, TS-1 was modified with polydopamine (TS-1@PDA). Using TS-1@PDA as a matrix, organic substances in the low-molecular-weight region such as amino acids, nucleosides, peptides, oligosaccharides, and fatty acids can be detected by MALDI-MS in positive ion mode. Compared with traditional organic matrices like 2,5-dihydroxybenzoic acid (2,5-DHB) and α-cyano-4-hydroxycinnamic acid (CHCA), TS-1@PDA has the advantages including the formation of uniform sample spots, small background interference at low molecular weight, and better salt tolerance. Furthermore, this matrix was employed for the analysis of endogenous glucose in urine samples, and the level of glucose was quantified with a linear range of 0–10 mM (R2 > 0.98). The results demonstrated that TS-1@PDA has the potential to be used as an effective MALDI matrix for the analysis of small molecules in biological samples with excellent reproducibility and moderate sensitivity.
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