TALANTA
·
2018
·
IF 4.244
Weiwei Wang,Suqin Liu,Chengjie Li,Yan Wang,Chao Yan
A novel dual-target recognition sandwich strategy for selective capture and detection of MCF-7 breast cancer cells based on core-shell magnetic mesoporous silica (Fe 3 O 4 @nSiO 2 @mSiO 2 @apt) nanoparticles was developed. Fe 3 O 4 @nSiO 2 @mSiO 2 @apt nanoparticles, which were prepared by a layer-by-layer method and were used for the first time to capture cancer cells, have large surface areas, particularly accessible mesochannels, and good biocompatibility, enabling aptamers to be compactly anchored onto the surface of the core-shell magnetic nanoparticles . A mucin 1 protein (MUC1)-targeted Fe 3 O 4 @nSiO 2 @mSiO 2 @apt nanoparticle was used as an affinity magnetic isolate material to capture target MCF-7 cells selectively and to reduce interference through affinity interaction between the anti-MUC1 aptamer and the MUC1 protein over-expressed on the surface of the MCF-7 cells. Meanwhile, a folate receptor (FR)-targeted affinity fluorescent probe (FA-BSA-FITC) was developed by coupling folic acid and FITC to the surface of BSA, enabling high sensitivity, selective fluorescent labeling of FR over-expressed MCF-7 cells. A dual-target recognition sandwich assay was developed based on the MUC1-targeted magnetic nanoparticles and the FR-targeted fluorescent probes . Under optimum conditions, a quantitative assay of MCF-7 cells was achieved with a dynamic range of 10 2 –10 5 cells/mL (R 2 = 0.9991). This assay showed high specificity and sensitivity to the target MCF-7 cells. Finally, the proposed strategy could be extended to detect MCF-7 cells in human plasma and whole blood with a recovery range of 86.1–104.0% and a RSD range of 1.2–8.4%, respectively. This indicates that the dual-target recognition method developed in this research exhibits good selectivity , anti-interference capability, and reliability even in plasma and whole blood samples and is more suitable for complex samples than previous targeted assays. Therefore, the approach proposed here may have great potential for early breast cancer diagnosis.
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高影响因子
Catalysis Science & Technology
·
2018
·Vol. 8
·期 21
·5506-5514
·
IF 5.365
Wanbing Gong,Chun Chen,Haimin Zhang,Guozhong Wang,Huijun Zhao
N-Doped carbon nanotube-encapsulated metal nanoparticles are of great interest in heterogeneous catalysis owing to their improved mass transfer ability and superior stability. Herein, a facile one-pot pyrolysis approach using melamine as the carbon and nitrogen source was developed to fabricate metal nanoparticles embedded in bamboo-like N-doped carbon nanotubes (named as Co@NCNTs-600-800 and Ni@NCNTs-600-800). The optimized Co@NCNTs-600-800 catalyst exhibited outstanding activity in furfural (FAL) selective hydrogenation to furfuryl alcohol (FOL) or cyclopentanone (CPO) in aqueous media. High yields of FOL (100%) and CPO (75.3%) were achieved at 80 °C and 140 °C, respectively. Besides, this cobalt catalyst showed very good stability and recyclability during the reaction. The synergistic effect between metallic cobalt and N-doped carbon nanotubes was systematically investigated. In addition, the as-prepared Ni@NCNTs-600-800 catalyst also exhibited remarkable activity. Under optimal conditions (100 °C and 4 MPa H2 pressure), a maximum tetrahydrofurfuryl alcohol (THFOL) yield (99.5%) was obtained in the aqueous-phase hydrogenation of FAL. The research thus highlights new perspectives for non-noble metal-based N-doped carbon nanotube catalysts for biomass transformation.
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CHEMISTRY-A EUROPEAN JOURNAL
·
2018
·Vol. 24
·期 64
·17148-17154
·
IF 5.16
Hong Dong,Gui-Xin Yang,Xin Zhang,Xiang-Bin Meng,Jing-Li Sheng,Xiao-Jun Sun,Yu-Jie Feng,Feng-Ming Zhang
Graphical Targeted drug delivery : Nanoscale metal–organic frameworks modified with folic acid (FA-NMOFs) have been prepared as potential drug carriers for targeted delivery to tumor cells (see figure). The anticancer drug 5-fluorouracil (5-FU) was encapsulated in the pores of the FA-NMOFs. The 5-FU-loaded FA-NMOFs showed excellent biocompatibility and were found to effectively kill tumor cells through the targeting effect of the FA-NMOFs. Nanoscale metal–organic frameworks (NMOFs) have proven to be a class of promising drug carriers as a result of their high porosity, crystalline nature with definite structure information, and potential for further functionality. However, MOF-based drug carriers with active tumor-targeting function have not been extensively researched until now. Here we show a strategy for constructing active tumor-targeted NMOF drug carriers by anchoring functional folic acid (FA) molecules onto the metal clusters of NMOFs. Two zirconium-based MOFs, MOF-808 and NH 2 -UiO-66, were chosen as models to reduce to the nanoscale for application as drug carriers, and then the terminal carboxylates of FA molecules were coordinated to Zr 6 clusters on the surfaces of the nanoparticles by substitution of the original formate or terminal -OH ligands. The successful modification with FA was confirmed by solid-state 13 C MAS NMR and UV/Vis spectroscopy and other characterization methods. Drug loading and controlled release behavior at different pH were determined by utilizing the anticancer drug 5-fluorouracil (5-FU) as the model drug. Confocal laser scanning microscopy measurements further demonstrated that 5-FU-loaded FA-NMOFs have excellent targeting ability through the efficient cellular uptake of FA-NMOFs. This work opens up a new avenue to the construction of active tumor-targeted NMOF-based drug carriers with potential for cancer therapies.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 7
·4981-4990
·
IF 3.201
Min Wang,Ke-yan Zheng,Shao-wu Lv,Hai-feng Zou,Hong-sen Liu,Gang-lin Yan,Ai-dong Liu,Xiao-fang Fei
Besides having many important physiological functions, cell membrane proteins have been recognized as major markers for many diseases in clinical examination. The discovery of new cell membrane proteins has attracted significant research interest. Herein, a multifunctional Fe3O4@SiO2/CdTe nanocomposite was designed for the detection and separation of cell membrane proteins, which was synthesized by a covalent coupling reaction between amino groups and carboxyl groups. It can serve as a universal tool for modifying antigens or other molecules that have amino groups. To verify the feasibility and practicability of this method, a folic acid (FA)-functionalized magnetic/fluorescent nanoprobe (Fe3O4@SiO2/CdTe-NH-FA) has been developed for recognizing and separating the FA receptor on HepG2 cells. FA was fixed to the shell of Fe3O4@SiO2/CdTe nanocomposites via 1,6-hexamethylenediamine. It obviously reduces the fluorescence quenching effect of FA on magnetic/fluorescent nanocomposites. The results demonstrate that the synthesized nanoprobe Fe3O4@SiO2/CdTe-NH-FA provides excellent optical characteristics for the reliable imaging of cancer cells and has good magnetic properties to enrich and isolate cell membrane proteins. Consequently, this biological nanoprobe can be used to find, obtain, and study unknown membrane proteins and predict the occurrence of some diseases. In addition, this nanoprobe will be a potential platform for accurate cancer diagnosis and therapy in the future.
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ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Chengxin Luan,Huan Wang,Qi Han,Xiaoyan Ma,Dagan Zhang,Yueshuang Xu,Baoan Chen,Minli Li,Yuanjin Zhao
Recovery of circulating tumor cells (CTCs) from cancer patients by an efficient CTCs capture and release method can greatly increase their application in diagnostics and treatment of cancers. In this paper, we presented a folic acid (FA)-functionalized hybrid photonic barcode for capture and release of CTCs. The hybrid photonic barcodes were formed by two nano-ordered parts, poly(ethylene glycol) diacrylate (PEGDA) inverse opal structure for sustaining integrity and methacrylated gelatin (GelMA) gel filler for conjugating FA molecules to mediate cell capture. The nano-ordered structures of the hybrid photonic barcodes not only increased contact area, but also decreased steric hindrance among FA molecules. Thus, the topographic interaction between the barcodes and CTCs was greatly enhanced. In addition, GelMA gel was soft and extracellular matrix (ECM) alike, which was beneficial to decrease impairment to CTCs during the CTCs-barcode interaction as well as preserving their viability. Demonstrated by four CTCs types, Hela (epithelial tissue, folate receptor positive, FR+), A02 (bone marrow, FR+), Raji (lymphoid tissue, FR+), and A549 (epithelial tissue, folate receptor negative, FR-), FR+ CTCs could be captured efficiently with reliability and specificity. The captured cells could be controllably released with high viability just by quick trypsinization. The whole processes were simple and efficient. These features indicated that the FA-functionalized hybrid photonic barcodes were promising for full recovery of CTCs from cancer patients, which was important for diagnosis and treatment of cancer.
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高影响因子
Materials Chemistry Frontiers
·
2018
·Vol. 2
·期 8
·1529-1538
·
IF 0
Lijing Niu,Feiyan Zhu,Bowen Li,Lingling Zhao,Hongze Liang,Yinghua Yan,Hui Tan
A folate-conjugated and pH-sensitive polymeric micellar system for the co-delivery of DOX and PTX was studied. A doxorubicin conjugated prodrug was synthesized via Schiff's base reaction. Subsequently, folate was grafted onto the prodrug and PTX was encapsulated. Sustained drug release of both DOX and PTX from the polymeric micelles was observed and the release rate could be accelerated by decreasing the media pH. A cellular uptake assay revealed that the polymeric micelles were internalized in the cytoplasm via endocytosis by SW1353 cells, and the cellular uptake was enhanced for the folate-conjugated micelles due to an active FR-mediated endocytosis pathway, showing stronger red fluorescence compared to that of non-folate micelles. The in vitro anticancer efficiency of the polymeric micellar system was evaluated using a cytotoxicity assay by incubating different drug formulations with the SW1353 cells. Both free drugs and micellar formulations displayed inhibition of cell growth at different levels, while the folate-conjugated dual-drug loaded polymeric micelles (Folate–Oxd–DOX/PTX) displayed a much lower IC50 value than other drug formulations, indicating a desirable in vitro anticancer efficiency due to the synergistic effect of co-delivery and active targeting. Thus, the polymeric micellar system is a promising platform for targeted cancer chemotherapy.
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JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2018
·Vol. 18
·期 6
·4196-4203
·
IF 1.354
Wu Jinzhu,Feng Yaxiu,Shao Yanbin,Sun Yanchun
Nitrogen and silicon co-doped carbon nanodots (N/Si-CDs) were synthesized by one-step hydrothermal carbonization of folic acid and 3-aminopropyl trimethoxy silane with the assistance of glycerol. The resultant N/Si-CDs present intense excitation-independent photoluminescence (PL) emission
owing to their monodisperse sizes and specific surface states. In comparison to nitrogen single doped CDs (N-CDs), the co-doped N/Si-CDs possess better PL properties following different emission manner due to the synergistic effects of the nitrogen and silicon dopants. Besides, the functionalized
surfaces of N/Si-CDs endow them with superior solubility and stability. The as-synthesized N/Si-CDs demonstrate selective and sensitive fluorescence response to Fe3+, which could be explained by the PL quenching effects of Fe3+ on N/Si-CDs. Based on this finding, the
quantitative analysis of aqueous Fe3+ by N/Si-CDs was established, showing good linearity in a range of 10 nM~45 μM and good sensitivity with a detection limit of 3.8 nM, so did for the detection of Fe3+ in fetal bovine serum (FBS) spiked samples. Therefore,
the N/Si-CDs as a novel fluorescence probe hold promising for monitoring environmental and clinical Fe3+.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2018
·Vol. 34
·期 2
·203-211
·
IF 1.248
Song Shengmei,Li Minglu,Gong Xiaojuan,Han Hui,Zhou Yehong,Wang Li,Shuang Shaomin,Dong Chuan
In the paper, folic acid(FA)-mediated and β -cyclodextrin( β -CD) derivatives functionalized magnetic Fe 3 O 4 nanoparticles(MNPs) were successfully prepared as drug carriers for the targeted delivery and controlled release of water-insoluble anticancer drug. FA-sulfobutyl ether- β -CD-MNPs(FA-SBE- β -CD-MNPs), FA-hydroxypropyl- β -CD-MNPs(FA-HP- β -CD-MNPs) and FA-carboxymethyl- β -CD-MNPs(FA-CM- β -CD-MNPs) were fabricated, and the loading efficiency and relative entrapment rate of curcumin are 12.04 mg/g, 95.56% for FA-SBE- β -CD-MNPs, 9.6 mg/g, 81.63% for FA-HP- β -CD-MNPs and 7.88 mg/g, 85.28% for FA-CM- β -CD-MNPs, respectively. Meanwhile, at pH=5.0, the optimal release rate of curcumin is about 46.07% for FA-SBE- β -CD-MNPs in 5 h. Cellular uptake indicates that curcumin can be selectively transported to targeting site and released from the internalized carriers. The in vitro cytotoxicity reveals that non-toxic FA-SBE- β -CD-MNPs have excellent biocompatibility on HepG2 cells in the tested concentrations. Therefore, FA-SBE- β -CD-MNPs could provide a promising platform for the targeting delivery of water insoluble anti-cancer drugs for different treatment needs of cancer therapy.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Zihao Zhang,Zehua Pei,Hao Chen,Kequan Chen,Zhaoyin Hou,Xiuyang Lu,Pingkai Ouyang,Jie Fu
Al2O3-supported Cu and Ni monometallic as well as Cu–Ni bimetallic catalysts were synthesized using a coprecipitation method and studied for the in-situ hydrogenation of furfural (FAL) with isopropanol as the solvent and hydrogen donor. The Cu–Ni bimetallic catalysts showed improved activity toward the production of 2-methylfuran (2-MF) and 2-methyltetrahydrofuran (2-MTHF) over that of monometallic catalysts. The results indicated that isopropanol exhibited better performance than methanol for the in-situ hydrogenation of FAL to produce 2-MF and 2-MTHF under the same conditions. The reaction conditions such as the copper–nickel ratios, catalyst loading amount, reaction temperature, and time were optimized. After the reaction was complete, the supported Cu–Ni bimetallic catalyst could be reused four times without a significant loss in catalytic activity.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2018
·
IF 3.997
Jie Zhang,Xiuli Ren,Xiaohan Tian,Peng Zhang,Zhenhua Chen,Xun Hu,Xifan Mei
Developing safe and effective stimuli-responsive nanocarriers is very important for tumor chemotherapy. In this work, bovine serum albumin (BSA) and green tea polyphenol (TP) were used to prepare glutathione (GSH) and enzyme (trypsin) responsive nanocarriers for doxorubicin (DOX). These nanocarriers were further modified with folate, briefly named as DOX@BSA-TP-FA NSs. The diameter of nanocarriers was about 220 nm. The DOX loading efficiency and loading amount were 86.4% and 23.5 wt%, respectively. The cellular uptake, apoptosis, and GSH and trypsin responsive release properties of these nanocarriers were investigated.
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高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2018
·Vol. 19
·期 10
·3132
·
IF 3.687
Liang Li,Na Liang,Danfeng Wang,Pengfei Yan,Yoshiaki Kawashima,Fude Cui,Shaoping Sun
The present investigation aimed to develop a tumor-targeting drug delivery system for paclitaxel (PTX). The hydrophobic deoxycholic acid (DA) and active targeting ligand folic acid (FA) were used to modify water-soluble chitosan (CS). As an amphiphilic polymer, the conjugate FA-CS-DA was synthesized and characterized by Proton nuclear magnetic resonance (1H-NMR) and Fourier-transform infrared spectroscopy (FTIR) analysis. The degree of substitutions of DA and FA were calculated as 15.8% and 8.0%, respectively. In aqueous medium, the conjugate could self-assemble into micelles with the critical micelle concentration of 6.6 × 10−3mg/mL. Under a transmission electron microscope (TEM), the PTX-loaded micelles exhibited a spherical shape. The particle size determined by dynamic light scattering was 126 nm, and the zeta potential was +19.3 mV. The drug loading efficiency and entrapment efficiency were 9.1% and 81.2%, respectively. X-Ray Diffraction (XRD) analysis showed that the PTX was encapsulated in the micelles in a molecular or amorphous state. In vitro and in vivo antitumor evaluations demonstrated the excellent antitumor activity of PTX-loaded micelles. It was suggested that FA-CS-DA was a safe and effective carrier for the intravenous delivery of paclitaxel.Keywords:chitosan;deoxycholic acid;folic acid;amphiphilic polymer;micelles;paclitaxel
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xin Hai,Yiting Wang,Xiaoyao Hao,Xuwei Chen,Jianhua Wang
Folic acid (FA) encapsulated graphene quantum dots (FA-GQDs) were prepared via a one-pot microwaved alkali cutting method, using graphene oxide (GO) and FA as raw material. TEM and AFM images revealed that FA was successfully cross-linked on the surface of GQDs under alkali circumstance, giving rising to stable and homogeneous FA-GQDs with size of about 33.6 nm. XPS and FT-IR confirmed that there are abundant hydroxyl, carboxyl and amino groups on the encapsulated FA-GQDs. The as-prepared FA-GQDs possessed the fluorescence behaviors of multi-excitation and emission sites, and exhibited sensitive response to the pH variation within the pH range of 3–9, making it excellent fluorescence probe for pH sensing in cell lysate and environmental water samples. The broad emission with pH-sensitive characteristic and low toxicity of FA-GQDs also provided an alternative for the specific multicolor imaging and sensing of pH in living cells.
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