MOLECULES
·
2024
·Vol. 29
·期 9
·2043
·
IF 4.2
Ju Liu,Wenfeng Li,Lu Jin,Yingchao Wang,Xinjun Xu,Enyao Ma,Depo Yang,Zhimin Zhao
Two unreported heteropolysaccharides, denoted as YCJP–1 and YCJP–2, were isolated from the herbs ofChloranthus japonicus. YCJP–1 was a heteropolysaccharide composed of glucose, galactose, arabinose, mannose, rhamnose, and a minor proportion of uronic acids, with the molecular weight mainly distributed in the 74,475–228,443 Da range. YCJP–2 was mainly composed of glucose, mannose, and galactose, with the molecular weights ranging from 848 to 5810 Da. To further evaluate the anti-gastric cancer effects ofC. japonicus, the inhibitory effects of the crude polysaccharide (YCJP) and the purified polysaccharides (YCJP–1 and YCJP–2) were determined using a CCK-8 assay and colon-forming assay on MGC-803 and AGS gastric cancer cell lines. Our results showed that YCJP, YCJP–1, and YCJP–2 possess prominent inhibitory effects on the proliferation of MGC-803 and AGS cells, and the AGS cell was more sensitive to YCJP, YCJP–1, and YCJP–2. Moreover, YCJP–2 demonstrated superior anti-gastric cancer effects compared to YCJP–1. This could potentially be attributed to YCJP–2’s higher glucose content and narrower molecular weight distribution.
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ENERGY
·
2024
·
IF 9
Chao Wang,Xiangrong Wu,Ao Xia,Abdul-Sattar Nizami,Yun Huang,Xianqing Zhu,Xun Zhu,Qiang Liao
Anaerobic digestion (AD) is an effective means to convert biomass hydrolysate to biomethane for bioenergy utilization. However, byproducts such as furfural and 5-hydroxymethylfurfural may be generated from soluble sugars during the hydrothermal pretreatment of lignocellulosic biomass, potentially inhibiting subsequent AD. Emerging electro-anaerobic digestion technology may be effective in releasing the inhibition. In this study, various voltages (0.3−1.2 V) were applied for the first time in the AD of lignocellulose hydrolysate. The results revealed that maximum chemical oxygen demand (COD) removal efficiency of 95.5 % occurred at 0.9 V, outperforming the voltage absence control by 22.5 %. Meanwhile, the most significant methane yield (336.9 NmL/g COD) and energy yield (12.1 kJ/g COD) were also achieved at 0.9 V, exceeding the control by 60.1 %. At 0.3, 0.6, and 1.2 V, AD performance outperformed that of the voltage absence, although it was not optimal due to either inadequate potential drive or excess-voltage suppression. The imposed voltage stimulated the growth of biofilm and tryptophan and humic acid secretion, enhancing the electrochemical activity. The more functionally selective electroactive bacteria Petrimonas and Sedimentobacter and archaebacteria Methanosarcina and Methanomassiliicoccus were significantly enriched under applied voltages. Efficient reciprocal metabolism among electroactive microorganisms was promoted to diversify methane production pathways.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yuchao Shao,Jianchao Wang,Zhiqiang Dong,Yujie Wang,Wenjing He,Weizhong Huo,Rong Ye,Yanqing Liu,Kexue Liu,Jialin Xue,Wenjing Lu,Tianxue Yang
Biomass-derived hydrochar exhibits significant potential for producing hydrothermal humic acid (HHA) in an alkaline hydrothermal environment. However, the quantitative relationship between the chemical characteristics of hydrochar and HHA yield remains unclear, and a method for assessing the hydrothermal humification potential of lignocellulosic biowaste under complex conditions is lacking. This study addressed this knowledge gap by identifying three scenarios based on the key indicator “the unsaturation degree of lignocellulosic biowaste’s and hydrochar’s average molecular formula (defined as Ω 1 and Ω 2 , respectively)”: (1) insufficient hydrothermal carbonization (Ω 2 − Ω 1 < 1), (2) sufficient hydrothermal carbonization (Ω 2 − Ω 1 ≥ 1 and Ω 2 ≤ 4), and (3) excessive hydrothermal carbonization (Ω 2 ≥ 4). Using these scenarios, the study compiled data from literature and established a database (approximately 500 data sets) on the hydrothermal humification of lignocellulosic biowaste via hydrochar. Subsequently, the study applied a machine learning approach—Particle Swarm Optimization and Least Squares Support Vector Machine (PSO-LS-SVM)—to predict HHA yield based on different raw materials and complex hydrothermal conditions. The MAE, MSE, RMSE, and R 2 values from 50 random PSO-LS-SVM models demonstrated high accuracy and stability in predictions, with the highest R 2 values reaching 0.96 (scenario 1) and 0.97 (scenario 2). This approach can significantly reduce costs and effort for users conducting biowaste hydrothermal humification experiments.
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Frontiers in Sustainable Food Systems
·
2024
·
IF 3.7
Weiwei Xu,Wei Zhou,Jinfeng Sun,Weiwei Chen,Xuanye Wu,Tong Guan,Yilin Zhao,Pengcheng Yang,Zhengyu Hu,Gao Li
The purpose of this study was to obtain polysaccharides from Panax ginseng C. A. Meyer stems (PGSPs), agro-byproducts with development potential, and fully explore the potential value in P. ginseng stems. Two novel polysaccharides firstly from P. ginseng stems (PGSP-1 and PGSP-2) were obtained by water extraction and alcohol precipitation method and column chromatography, and then characterized by FT-IR, HPGPC, HPLC, SEM, TGA, GC–MS and NMR. The results demonstrated that PGSP-1 (Mw = 723.83 kDa) and PGSP-2 (Mw = 620.48 kDa) were characterized →4)-β-D-Galp-(1→, →6)-α-D-Glcp-(1 → and →2)-α-L-Rhap-(1 → as the skeleton, →4,6)-β-D-Galp-(1 → and →2,6)-α-D-Manp-(1 → as the cross junction, α-L-Araf-(1 → as the terminal unit, and PGSP-1 still contained →4)-β-D-Galp, while PGSP-2 contained →4)-β-D-Xylp-(1→, →3)-β-D-GlcpA-(1→, →4)-β-D-GalpA-(1 →) and α-D-Glcp-(1→, with different microstructures and thermal stability. And the results of hypoglycemic activity revealed that both PGSP-1 and PGSP-2 showed excellent inhibitory activity against α-glucosidase and α-amylase, in which PGSP-2 had the better performance. The inhibition kinetics result showed that PGSPs on α-glucosidase and α-amylase were non-competitive type and mixed type inhibition. This study provided a theoretical basis for making full use of and exploiting the economic value of agro-byproducts such as P. ginseng stems and offered a theoretical reference for the effective utilization of PGSP as a functional component to prevent and reduce T2DM.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Sheng-Jie Chen,Ming-Jun Zhu,Si-Ying Cui
High salinity waste, such as food waste, is difficult for microbial utilization as high salt increases cellular osmosis. In this study, a novel cellulolytic halotolerant (<150 g/L salinity) strain Clostridium thermopalmarium HCD (halotolerant and cellulose-degrading strain) was screened from paper sludge, which obtained 108.47 and 42.53 mM hydrogen from cellobiose under 30 and 80 g/L NaCl respectively. HCD could improve 25.14 % hydrogen production of MT (a mixed thermophilic Clostridiales ) consortium from co-substrates of pretreated sugarcane bagasse and food waste as bio-enhancer. Microbial community analysis showed that the inoculation of HCD profoundly changed the original community structure and dominant species of MT with the prolongation of fermentation time, leading to increase utilization of co-substrates and higher hydrogen production. In response to high salinity, HCD regulated the cell shape from a slender rod to a stubby one, balancing the high osmotic pressure. Furthermore, HCD could secrete and transport betaine as the main response to high salinity. Betaine could mediate the Na/K pump-like transport system, especially Na + /H + antiporter to balance the intracellular osmotic pressure. Betaine could also relieve cell membrane damage, improving the transportation of extracellular P ions to the intracellular. Moreover, betaine could increase the levels of extracellular polymers and intracellular ATP. This study provided a halotolerant hydrogen-producing strain and its halotolerant mechanism, which paid a solid foundation for the utilization of high-salt waste biomass.
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Journal of Cleaner Production
·
2024
·
IF 9.7
Yikui Zhu,Long Li,Yansu Hou,Chuan Xu,Guoxiang Hu,Yi Man,Vasilevich Sergey Vladimirovich,Jun Li,Qingang Xiong
Pulp molded generated from renewable bagasse as biodegradable packaging materials has been widely viewed as an eco-friendly and cheap way to substitute the current huge consumption of nondegradable plastics, and deep eutectic solvents (DES) pretreatment are a promising approach to obtain high-yield pulp efficiently and sustainably. However, mainstream binary-component DES still suffers issues of high viscosity and corrosiveness. This study put forward a ternary-component DES (3C-DES) with the addition of ethylene glycol (EG) into choline chloride (ChCl) and P-toluenesulfonic acid (PTSA) and examined pretreatment performance and optimal DES ratio for thread rolling bagasse via compositional analysis and physicochemical characterization. Moreover, density functional theory calculations were used to reveal structures and weak interactions of different ratios of 3C-DES, and a novel 3C-DES pretreatment chemical-mechanical pulp and pulp molded were finally prepared. The results indicate that the 3C-DES with ChCl:PTSA:EG molar ratio of 1:1:3 is most effective, evidenced by more than 97.94% lignin removals. The 3C-DES pretreated mechanical pulp exhibits superior fiber properties, whereas the hot-pressed pulp molded shows excellent physical properties and thermal stability. Thus, the proposed 3C-DES has great potential to obtain high-yield high-performance pulp molded with low cost and environmental sustainability.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2024
·Vol. 47
·期 6
·957-969
·
IF 3.5
Zou Xiao-Zhou,Gong Lu-Chan,Li Ting-Ting,Lv Shu-Yi,Wang Jun
γ-Aminobutyric acid (GABA) is a crucial neurotransmitter with wide application prospects. In this study, we focused on a GABA-producing strain from a traditional Chinese fermented beverage system. Among the six isolates, Lactobacillus hilgardii GZ2 exhibited the greatest ability to produce GABA in the traditional Chinese fermented beverage system. To increase GABA production, we optimized carbon sources, nitrogen sources, temperature, pH, and monosodium glutamate and glucose concentrations and conducted fed-batch fermentation. The best carbon and nitrogen sources for GABA production and cell growth were glucose, yeast extract and tryptone. Gradual increases in GABA were observed as the glucose and monosodium glutamate concentrations increased from 10 g/L to 50 g/L. During fed-batch fermentation, lactic acid was used to maintain the pH at 5.56, and after feeding with 0.03 g/mL glucose and 0.4 g/mL sodium glutamate for 72 h, the GABA yield reached 239 g/L. This novel high-GABA-producing strain holds great potential for the industrial production of GABA, as well as the development of health-promoting functional foods and medical fields.
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FOOD CONTROL
·
2024
·
IF 5.6
Luyao Zhao,Jin Liu,Bing Bai,Yongpeng An,Yukun Yang,Xiaowen Wang,Xu Jing
Herein, we established a green, rapid, and sensitive method to analyze ochratoxin A (OTA) in cereals based on modified QuEChERS and enzyme-linked immunosorbent assay (ELISA). Lignocellulose is a renewable material mainly consisting of cellulose, hemicellulose, and lignin. In the modified QuEChERS, a natural deep eutectic solvent synthesized from glucose and levulinic acid derived from cellulose and hemicellulose was used as an extraction solvent to replace traditional organic solvents. Furthermore, modified lignin was used as an adsorbent to purify the extraction solution. In the magnetic bead-based ELISA, the effervescent reaction accelerated the immune competition. Additionally, magnetic beads were used to shorten the incubation and washing time, whereas gold nanoparticles were used to amplify the optical signal. This method has been successfully applied to the analysis of OTA in cereal samples. The limits of detection were 0.68–0.83 μg kg −1 . This pretreatment and detection strategy has potential application in analyzing mycotoxins.
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DESALINATION
·
2024
·
IF 8.3
Gao Xuerui,Li Ping,Qin Yuan,Bai Chengling,Gu Zhengyang,Yu Shuili
The negative rejection of certain solutes is an interesting phenomenon often observed in nanofiltration (NF) process, which presents an ideal solute separation effect. In this study, the NF membrane pore size, electric charge property and influent ions composition were controlled to investigate the rule and mechanism of negative rejection phenomenon during SO 4 2− /Cl − mixed salt NF. The results show that the changing of the membrane pore diameter and charge will significantly affect the NF selectivity to SO 4 2− /Cl − , then affect the negative rejection phenomenon. Selective efficiency of Cl − /SO 4 2− can be up to 156.04–283.20 for the negative charged NF membrane with an average pore diameter of 0.62–0.68 nm, and the Cl − was negatively rejected under many influent conditions with the lowest rejection rate of −252.36 %. The substitution of Na + for Mg 2+ and the increase of SO 4 2− /Cl − ratio are beneficial to improve selectivity. The Donnan Steric Pore Model with dielectric exclusion model was used to calculate the rejection curve which is consistent with the experimental results. The analysis of NF mechanism shows that the negative rejection of Cl − is caused by the extra transfer power derived from the NF selectivity to Cl − , which is a compensation for the higher rejection rate of SO 4 2− .
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Biomolecules
·
2024
·Vol. 14
·期 9
·1188
·
IF 4.8
Jiao Ma,Zhongke Sun,Zifu Ni,Yanli Qi,Qianhui Sun,Yuansen Hu,Chengwei Li
This study identified a salt-tolerant GH11 xylanase, Xyn st , which was isolated from a soil bacterium Bacillus sp. SC1 and can resist as high as 4 M NaCl. After rational design and high-throughput screening of site-directed mutant libraries, a double mutant W6F/Q7H with a 244% increase in catalytic activity and a 10 °C increment in optimal temperature was obtained. Both Xyn st and W6F/Q7H xylanases were stimulated by high concentrations of salts. In particular, the activity of W6F/Q7H was more than eight times that of Xyn st in the presence of 2 M NaCl at 65 °C. Kinetic parameters indicated they have the highest affinity for beechwood xylan ( K m = 0.30 mg mL −1 for Xyn st and 0.18 mg mL −1 for W6F/Q7H), and W6F/Q7H has very high catalytic efficiency ( K cat / K m = 15483.33 mL mg −1 s −1 ). Molecular dynamic simulation suggested that W6F/Q7H has a more compact overall structure, improved rigidity of the active pocket edge, and a flexible upper-end alpha helix. Hydrolysis of different xylans by W6F/Q7H released more xylooligosaccharides and yielded higher proportions of xylobiose and xylotriose than Xyn st did. The conversion efficiencies of Xyn st and W6F/Q7H on all tested xylans exceeded 20%, suggesting potential applications in the agricultural and food industries.
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FOOD CHEMISTRY
·
2018
·
IF 4.946
Lei Wang,Bin Zhang,Jie Xiao,Qiang Huang,Chao Li,Xiong Fu
Water-soluble polysaccharides (RTFP) were extracted from Rosa roxburghii Tratt fruit by hot water method. The physicochemical, functional, and hypoglycemic properties of RTFP were investigated. The results revealed that RTFP mainly contained carbohydrates (63.79 ± 0.73%, g/g), uronic acids (14.8 ± 0.06%, g/g), and proteins (4.10 ± 0.58%, g/g). RTFP was composed of arabinose, galactose, glucose, mannose, xylose, and fucose with molar percentages of 33.8, 37.3, 20.7, 1.74, 3.43, and 2.95%, respectively. Functional analyses indicated that RTFP had good oil-holding capacity, foaming properties, and emulsifying capacity. The rheological results showed that RTFP exhibited typical shear-thinning behavior and viscoelastic properties influenced by sample concentration, temperature and inorganic salt. Additionally, RTFP exhibited favorable inhibitory activities against α-glucosidase in a mixed inhibition type, and against α-amylase in an uncompetitive inhibition type. These results suggest that RTFP can be exploited as a multi-functional additive or hypoglycemic agent in foods, pharmaceuticals and cosmetics.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Jun Yuan,Shuxian Chen,Liping Wang,Tingting Xu,Xu Shi,Yi Jing,Haijiang Zhang,Yange Huang,Ying Xu,Dong Li,Xing Chen,Jianhui Chen,Qingping Xiong
The aim of this paper was to prepare purified fractions of polysaccharides from Monetaria moneta Linnaeus and further compare their characteristics and antioxidant activities. Firstly, three novel purified fractions, named MM-P1, MM-P2 and MM-P3, were successfully prepared by a DEAE-Sepharose fast-flow column. Then, their characteristics were compared using chemical testing, FT-IR, GC and HPGPC. The results suggested that MM-P3 had higher molecular weights than MM-P1 and MM-P2. MM-P1 was consisted of glucose, MM-P2 was consisted of glucose and xylose, and MM-P3 was comprised of glucose, xylose and mannose. Differed from MM-P1 and MM-P2, MM-P3 had sulfuric radical and uronic acid groups. Finally, their antioxidant activities were also compared. We found that MM-P3 exhibited better antioxidant bioactivities than MM-P1 and MM-P2. The data demonstrated that three purified fractions derived from different adsorption capacity of DEAE-Sepharose fast-flow column possessed different structural characteristics and antioxidant activity.
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危险品化学品经营许可证（带存储）