Food Analytical Methods
·
2023
·Vol. 16
·期 6
·1131-1144
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IF 2.9
Yu Chen,Luo Xiaogang,Wu Fengshou,Zhang Juan
A covalent organic framework/reduced graphene oxide composite (COF/rGO) was fabricated by facile ultrasonic self-assembly strategy and further coated onto stainless-steel wire by physically adhesion for headspace solid-phase microextraction (HS-SPME) of polycyclic aromatic hydrocarbons (PAHs) prior to their determination by gas chromatography-flame ionization detection (GC-FID). Compared with the individual porphyrin-based COF and GO, the as-prepared COF/rGO composite effectively prevented the stacking of GO nanosheets and exhibited enhanced extraction efficiency due to the synergistic effect, with the enrichment factor up to 8816 for phenanthrene. The morphology, structure, and stability of COF/rGO composite was characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermogravimetric analysis, and N 2 adsorption–desorption. Effective adsorption and desorption parameters including extraction temperature, extraction time, desorption temperature, desorption time, agitation speed, and ionic strength were optimized. The established HS-SPME/GC-FID method based on COF/rGO composite fiber was evaluated in terms of linear ranges from 0.5 to 250.0 ng mL −1 with R 2 ≥ 0.9985, limits of detection in the range of 0.09–0.59 ng mL −1 , and limits of quantitation in the range of 0.31–1.97 ng mL −1 . The intra-day, inter-day, and fiber-to-fiber relative standard deviations (RSDs) were in the range of 1.86–4.37%, 2.48–5.40%, and 3.51–7.81%, respectively. The established method was employed to determine trace level of PAHs in honey with good recoveries (62.99–128.8%) and reliable reproducibility (0.51–7.63%).
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ANALYTICA CHIMICA ACTA
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2023
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IF 6.2
Yang Liu,Xueping Dang,Huaixia Chen
A molecularly imprinted polymer (MIP) monolithic column was prepared in situ in a pipette tip using phenol and bisphenol A as dual templates, 4-vinyl pyridine and β -cyclodextrin as bifunctional monomers . It was used for the selective and simultaneous solid phase extraction of eight phenolics, including phenol, m-cresol, p-tert-butylphenol, bisphenol A, bisphenol B, bisphenol E, bisphenol Z, and bisphenol AP. The MIP monolithic column was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy , thermogravimetric analysis and nitrogen adsorption experiment. The results of selective adsorption experiments showed that the MIP monolithic column can selective recognize the phenolics and have excellent adsorption property. The imprinting factor for bisphenol A can be as high as 4.31, and the maximum adsorption capacity for bisphenol Z can reach 201.66 mg g −1 . Under the optimal extraction conditions, a selective and simultaneous extraction and determination method for eight phenolics was established based on the MIP monolithic column and high-performance liquid chromatography with ultraviolet detection. The linear ranges (LRs) of the eight phenolics were 0.5–200 μg L −1 , the limits of quantification (LOQs) and detection (LODs) were 0.5–2.0 μg L −1 and 0.15–0.67 μg L −1 . The method was applied to detect the migration quantity of the eight phenolics from polycarbonate cups and had satisfactory recovery. The method has the advantages of simple synthesis, short extraction time, as well as good repeatability and reproducibility, which provides a sensitive and reliable strategy for extracting and detecting phenolics from food contact material.
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ACS Applied Polymer Materials
·
2023
·
IF 5
Yan Qin,Weihua Su,Guihua Meng,Jianning Wu,Lin Cui,Shengchao Yang,Zhiyong Liu,Xuhong Guo
An intelligent responsive pesticide delivery system has been applied in agriculture to improve the utilization rate of pesticides. When plants are affected by fungi, a large number of reactive oxygen species (ROS) are produced rapidly on the wound surface. Based on this, a ROS responsive star polymer micellar slow-release system was constructed to load carbendazim (MBC) and improve its bioavailability. The star polymer was synthesized by using 3-mercaptopropionic acid, acetone, pentaerythritol (PER), ε-caprolactone (ε-CL), and methoxypolyethylene glycols (mPEG5000, Mw = 5000) and named PER-PCL-TK-mPEG5000. By loading MBC, the drug loading micelle MBC-loaded PER-PCL-TK-mPEG5000 was obtained. The cumulative release amount of MBC-loaded PER-PCL-TK-mPEG5000 containing H2O2 is 1.64 times of that without H2O2 after 120 h release. The inhibitory rate of MBC-loaded PER-PCL-TK-mPEG5000 containing H2O2 against Rhizoctonia solani (R. solani) was 91.6%. The critical micelle concentration (CMC) of PER-PCL-TK-mPEG5000 is 4.37 mg/L, and the result showed that PER-PCL-TK-mPEG5000 and MBC-loaded PER-PCL-TK-mPEG5000 have good stability at 4 °C for 14 d. This study proved that the strategy of designing pesticide vectors according to the target environment can be widely used to protect crops, improve pesticide utilization rate, and protect the environment.
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Advanced Science
·
2023
·
IF 15.1
Yuting Ji,Liya Sun,Yuan Liu,Yanhui Li,Tongxuan Li,Jiameng Gong,Xiali Liu,Huiqiang Ma,Jingying Wang,Bing Chen,Shan-Yu Fung,Hong Yang
Excessive activation of Toll-like receptor (TLR) signaling pathways and the circulating endotoxin are key players in the pathogenesis of many acute and chronic inflammatory diseases. Regulation of TLR-mediated inflammatory responses by bioactive nanodevices represents a promising strategy for treating these diseases. In searching for novel, clinically applicable nanodevices with potent TLR inhibitory activities, three types of hexapeptide-modified nano-hybrids with different cores of phospholipid nanomicelles, liposomes, and poly(lactic-co-glycolic acid) nanoparticles are constructed. Interestingly, only the peptide-modified lipid–core nanomicelles (M-P12) display potent TLR inhibitory activities. Further mechanistic studies disclose that lipid–core nanomicelles have a generic property to bind to and scavenge lipophilic TLR ligands including lipopolysaccharide to block the ligand–receptor interaction and down-regulate the TLR signaling extracellularly. In addition, the peptide modification enables M-P12 a unique capability to modulate endosomal acidification upon being endocytosed into macrophages, which subsequently regulates the endosomal TLR signal transduction. In an acute lung injury mouse model, intratracheal administration of M-P12 can effectively target lung macrophages and reduce lung inflammation and injuries. This work defines a dual mechanism of action of the peptide-modified lipid–core nanomicelles in regulating TLR signaling, and provides new strategies for the development of therapeutic nanodevices for treating inflammatory diseases.
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International Journal of Nanomedicine
·
2023
·
IF 8
Danrong Hu,Omar Mezghrani,Lei Zhang,Yi Chen,Xue Ke,Tianyuan Ci
Novel breast carcinoma dual-targeted redox-responsive nanoparticles (NPs) based on cholesteryl-hyaluronic acid conjugates were designed for intracellular delivery of the antitumor drug doxorubicin (DOX). A series of reduction-responsive hyaluronic acid derivatives grafted with hydrophobic cholesteryl moiety (HA-ss-Chol) and GE11 peptide conjugated HA-ss-Chol (GE11–HA-ss-Chol) were synthesized. The obtained conjugates showed attractive self-assembly characteristics and high drug loading capacity. GE11–HA-ss-Chol NPs were highly stable under conditions mimicking normal physiological conditions, while showing a fast degradation of the vehicle’s structure and accelerating the drug release dramatically in the presence of intracellular reductive environment. Furthermore, the cellular uptake assay confirmed GE11–HA-ss-Chol NPs were taken up by MDA-MB-231 cells through CD44- and epidermal growth factor receptor-mediated endocytosis. The internalization pathways of GE11–HA-ss-Chol NPs might involve clathrin-mediated endocytosis and macropinocytosis. The intracellular distribution of DOX in GE11–HA-ss-Chol NPs showed a faster release and more efficient nuclear delivery than the insensitive control. Enhanced in vitro cytotoxicity of GE11–HA-ss-Chol DOX-NPs further confirmed the superiority of their dual-targeting and redox-responsive capacity. Moreover, in vivo imaging investigation in MDA-MB-231 tumor-bearing mice confirmed that GE11–HA-ss-Chol NPs labeled with 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide, a near-infrared fluorescence dye, possessed a preferable tumor accumulation ability as compared to the single-targeting counterpart (HA-ss-Chol NPs). The antitumor efficacy showed an improved therapy efficacy and lower systemic side effect. These results suggest GE11–HA-ss-Chol NPs provide a good potential platform for antitumor drugs.
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Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Shiyu Bao,Likun Zhao,Yuanwen Liu,Lewei Yang,Peng Liu,Chaoqi Chen,Xuhui Mao
Polycyclic aromatic hydrocarbons (PAHs) are a group of contaminants with low bioavailability and biodegradability . As a result, removing PAHs from contaminated soils is often inefficient. In this study, soils spiked with fluoranthene and pyrene were inoculated with Aeromonas sp. BCP-3, a newly isolated strain from coke-plant sludge , and treated in slurry bioreactors . The removal of PAHs was significantly affected by aeration rate, slurry water-soil mass ratio, stirring rate, temperature, and the concentration of Tween-80. Such effects were attributed to changes in BCP-3 growth, PAHs solid-liquid mass transfer, and slurry particle size. Notably, under optimal conditions (i.e., aeration rate of 1.5 L/min, temperature of 30 °C, stirring rate of 200 rpm, water-soil mass ratio of 1.5:1, and Tween-80 concentration of 1.3 %), 96.3 % and 97.2 % of initially spiked fluoranthene and pyrene were removed within 12 days, respectively. A PAH-contaminated soil was collected from a local coking plant and treated under the same conditions, except for the Tween-80 concentration. For the real soil, the Tween-80 concentration was 4 % to ensure a significant proportion of PAHs released into the liquid phase. Twenty-one days after inoculation of BCP-3, the removal efficiencies of low molecular weight PAHs and high molecular weight PAHs were 82.2 % and 51.8 %, respectively. The removal efficiencies of PAHs were significantly higher than the slurry without BCP-3 or HgCl 2 -sterilized slurry. BCP-3 improved the bioavailability and biodegradability of PAHs, resulting in effective PAH removal in slurry bioreactors . Overall, successful bioaugmentation was achieved with the addition of Tween-80 and the optimization of operating conditions.
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Journal of Cleaner Production
·
2023
·
IF 11.1
Chun Wang,Qianqian Wang,Jintao Yu,Xinmeng Wang,Lijuan Wang,Bin Zhao,Lin Hao,Weihua Liu,Zhi Wang,Hao Chen,Qiuhua Wu
White pollution caused by the massive discharges of waste expanded polystyrene (WEPS) has become a critical global issue, so the effective conversion of WEPS is of great significance to reduce white pollution. Herein, adopting WEPS as raw material, three high-value-added hyper-crosslinked polymers (WEPS-HCPs) with high surface areas and abundant micro/mesoporous structures were synthesized via Friedel-Crafts alkylation reaction using dimethoxymethane, dichloroxylene and 4,4′-bis(chloromethyl)-1,1′-biphenyl (BCB) as cross-linker, respectively. The WEPS-HCPs were used as sorbent to extract aflatoxins (AFs) and all the HCPs exhibited high adsorption capacity . For the fast separation and recovery of the sorbent, WEPS-BCB was chosen as a model HCP to be magnetically functionalized to obtain a magnetic HCP (mWEPS-BCB) for the first time. Based on magnetic solid phase extraction with the mWEPS-BCB as sorbent, a fast and sensitive method was developed to determine trace level of AFs in grain food . Under the optimal conditions, the proposed method presented wide linear range (0.008–10.0 ng g −1 ), low detection limit (0.002–0.015 ng g −1 ) and satisfactory precision (relative standard deviation < 7%). Converting WEPS into a high-value-added sorbent not only provided a facile strategy for effective recycling of WEPS, but also developed a sensitive method for accurate determination of AFs in grain food, achieving significant economic and environmental benefits.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2023
·
IF 3
Chengjiang Zhang,Yuhuang Li,Hongmei Yuan,Zeyi Lu,Qi Zhang,Lirong Zhao
Covalent organic frameworks (COFs) are a burgeoning class of crystalline porous materials with unique properties and have been considered as a promising functional extraction medium in sample pretreatment. In this study, a new methacrylate-bonded COF (TpTh-MA) was well designed and synthesized via the aldehyde-amine condensation reaction, and the TpTh-MA was incorporated into poly (ethylene dimethacrylate) porous monolith by a facile polymerization reaction inside capillary to prepare a novel TpTh-MA monolithic column. The fabricated TpTh-MA monolithic column was characterized with scanning electron microscope, Fourier transform infrared spectrometer, X-ray diffraction, and N 2 adsorption–desorption experiments. Then, the homogeneous porous structure, good permeability and high mechanical stability of TpTh-MA monolithic column was used as separation and enrichment media of capillary microextraction, which was coupled with high-performance liquid chromatography fluorescence detection for online enrichment and analysis of trace estrogens. The main experimental parameters influencing the extraction efficiency were systematically investigated. The adsorption mechanism for three estrogens was also explored and discussed based on hydrophobic effect, π-π affinity and hydrogen bonding interaction, which contributed to its strong recognition affinity to target compounds. The enrichment factors of the TpTh-MA monolithic column micro extraction method for the three estrogens were 107–114, indicating a significant preconcentration ability. Under optimal conditions, a new online analysis method was developed and exhibited good sensitivity and wide linearity range of 0.25–100.0 µg·L -1 with a coefficient of determination ( R 2 ) higher than 0.9990 and a low limit of detection with 0.05–0.07 µg·L -1 . The method was successfully applied for online analysis of three estrogens of milk and shrimp samples and the recoveries obtained from spiking experiments were in range of 81.4–113% and 77.9–111%, with the relative standard deviations of 2.6–7.9% and 2.1–8.3% ( n = 5), respectively. The results revealed the great potential for the application of the COFs-bonded monolithic column in the field of sample pretreatment.
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GENE
·
2023
·
IF 3.5
Shaolin Xie,Yongyong Feng,Aiguo Zhou,Zhiyuan Lu,JixingZou
Cytochrome P450 1 (CYP1) is an important enzyme family involved in the metabolism of pollutants, and used as a biomarker to monitor environmental pollution. In this study, a fluorescence-labeled cyp1a zebrafish line, named as KI ( cyp1a +/+ -T2A-mCherry) (KICM), was originally constructed to monitor dioxin-like compounds in the environment. However, the cyp1a gene expression in the KICM line was inhibited by the fluorescence labeling, thus leading to a significantly increased sensitivity of KICM zebrafish line to PAHs. Then, a cyp1a knockout zebrafish line, named KOC, were constructed for comparative analysis with the cyp1a low-expression line. Interestingly, knockout of the cyp1a gene did not increase the sensitivity of zebrafish to PAHs as significantly as the cyp1a low-expression line. So, the expression levels of related genes in the aryl hydrocarbon receptor pathway were analyzed and the results showed that the expression level of cyp1b in KOC group was significantly higher than that of wild type and KICM under the same PAH exposure. This indicated that the effect of losing cyp1a was compensated by inducing expression of cyp1b . In conclusion, two new zebrafish models including cyp1a low-expression line and cyp1a knockout line were constructed in this study, which may provide a convenient model for subsequent studies on the toxicity mechanism of PAHs and the role of cyp1a in detoxification.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Maolin Wang,Yaling Wang,Xudong Jing,Jiahui Xu,Min Li,Qing Xie,Xiyun Cai
Iron-activated persulfate (PS) systems commonly suffer from the poor Fe(III)/Fe(II) redox cycle in groundwater and soils, due to the rapid oxidation of Fe(II) by both PS-related and naturally occurring oxidative substances. Maintaining the Fe(III)/Fe(II) redox cycle is necessary to achieve the high efficiency and stability of iron-activated persulfate systems toward aromatic contaminants (e.g., PAHs) in groundwater and soils. Herein, we discover the mixtures of dithionite (DTN) and zerovalent iron (ZVI) to synergistically activate PS, and develop highly efficient, stable DTN-ZVI/PS systems for PAHs removal in groundwater and soils. DTN and intermediates sulfite/thiosulfate initiated reduction of ZVI-derived Fe(III) species in DTN-ZVI/PS systems. This reaction accelerated the Fe(III)/Fe(II) redox cycle and coated ZVI with FeS x , both favoring the generation of HO and SO 4 − . DTN-ZVI/PS exhibited Nap removal of >90% in water during 11 of 15 supplementations of Nap and/or PS, 1.4–2.4 times ZVI/PS. Similarly, 0.47–4.3 fold increments of Nap removal k obs were obtained in five chemical types of groundwater, and 1.3–2.4 fold increments of PAHs removal in soils. This study may provide a technical basis to develop new Fe-activated oxidation systems for groundwater and soil remediation .
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EUROPEAN POLYMER JOURNAL
·
2023
·
IF 6
Mengting Du,Ying Yi,Yihua Yin,Zhi Cai,Weiquan Cai,Junli Li,Guanghua He,Jingli Zhang
Faced with increasingly serious drug resistance and environmental pollution, there is an urgent necessity to develop green and efficient pesticides with new mechanisms of action. A novel antibacterial nano-system for plant protection based on photodynamic action was prepared by grafting hematoporphyrin (HP) onto modified chitosan through ester bonding. The self-quenching effect caused by aggregation of grafted HP could effectively inhibit its photodegradation, thus enhancing its photostability. The breakage of ester bonds induced by enzyme stimulation at the sites of bacterial infection could trigger HP release exerting an efficient photodynamic effect. The antibacterial experiments showed that the nanoparticles (NPs) have good photodynamic antibacterial activity, and the inhibition rate was as high as 96 % after 60 min of light exposure. Adhesion and safety studies have shown that the NPs are well retained on plant foliage without causing damage to the crop. Thus, NPs have great potential in sustainable plant disease management.
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RSC Advances
·
2023
·Vol. 13
·期 17
·11782-11793
·
IF 3.9
Xiaoxiao Gu,Lixian Xu,Haoyang Yuan,Cailing Li,Juan Zhao,Shuang Li,Dinghua Yu
Anti-bacterial photodynamic therapy is the most promising treatment protocol for bacterial infection, but low accumulation of photosensitizers has seriously hindered their development in clinical application. Here, with inherent outstanding affinity to bacterial cell envelope, sophorolipid produced from Candida bombicola has been conjugated to toluidine blue (SL-TB) through amidation reaction. The structure of SL-TB conjugates was identified by 1H-NMR, FT-IR and ESI-HRMS. The interfacial assembly and photophysical properties of SL-TB conjugates have been disclosed through surface tension, micro-polarity, electronic and fluorescence spectra. After light irradiation, the log10 (reduced CFU) of free toluidine blue to P. aeruginosa and S. aureus were 4.5 and 7.9, respectively. In contrast, SL-TB conjugates showed a higher bactericidal activity, with a reduction of 6.3 and 9.7 log10 units of CFU against P. aeruginosa and S. aureus, respectively. The fluorescence quantitative results showed that SL-TB could accumulate 2850 nmol/1011 cells and 4360 nmol/1011 cells by P. aeruginosa and S. aureus, which was much higher than the accumulation of 462 nmol/1011 cells and 827 nmol/1011 cells of free toluidine blue. Through the cooperation of triple factors, including sophorose affinity to bacterial cells, hydrophobic association with plasma membrane, and electrostatic attraction, higher SL-TB accumulation was acquired, which has enhanced antibacterial photodynamic efficiencies.
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