AMB Express
·
2019
·Vol. 9
·期 1
·1-9
·
IF 2.226
Zhao Shuxue,Hu Chunhui,Guo Lizhong,Li Kuiran,Yu Hao
A 3-hydroxypyridine degrading bacterium, designated strain DW-1, was isolated from petroleum contaminated soil in Liao River China. 16S rRNA-based phylogenetic analysis indicates that strain DW-1 belongs to genus Agrobacterium. The optimal cultivation temperature and pH for strain DW-1 with 3-hydroxypyridine were 30 °C and 8.0, respectively. Under optimal conditions, strain DW-1 could completely degrade up to 1500 mg/L of 3-hydroxypyridine in 66 h. The 3-hydroxypyridine degradation pathway of strain DW-1 was suggested by HPLC and LC–MS analysis. The first reaction of 3-hydroxypyridine degradation in strain DW-1 was α-hydroxylation so that the major metabolite 2,5-dihydroxypyridine was produced, and then 2,5-dihydroxypyridine was transformed by a Fe2+-dependent dioxygenase to form N-formylmaleamic acid. N-Formylmaleamic acid will be transformed to maleic acid and fumaric acid through maleamic acid. This is the first report of the 3-hydroxypyridine degradation pathway and the utilization of 3-hydroxypyridine by a Agrobacterium sp. It may be potentially used for the bioremediation of environments polluted with 3-hydroxypyridine.
研究领域:
微生物学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2024
·
IF 4.5
Yaru Li,Qian Yang,Lanlan Fan,Fang Liu,Yaqi Shang,Baoxin Zhang,Zheng Wang,Yanjun Zhao
Ovarian cancers (OC) often resist current standard chemotherapy because of the cancer heterogeneity and over-expression of an anti-apoptotic protein, myeloid cell leukemia-1 (MCL-1). The heterogeneous OC shows impaired redox homeostasis and relies heavily on glutathione peroxidase 4 to eliminate toxic lipid peroxides. Therefore, integrated apoptosis and ferroptosis therapy is promising for OC management. The current work reports a liposomal nanosystem for apoptotic and ferroptotic OC therapy. Simvastatin (Sim) and vorinostat (Saha) were selected as the pleiotropic model drugs. The successive release of Saha and Sim from liposomes enabled the sequential amplification of MCL-1 by Saha, and then MCL-1 inhibition by Sim. Such a strategy could potently induce apoptosis in a human OC cell line (SKOV3), as indicated by increased intracellular cytochrome c concentration, caspase 3/9 activity, and the extent of apoptosis. Besides, Saha played multiple roles in cell death. It could induce cell cycle arrest at the G 2 /M phase and apoptosis. Saha also inhibited the glutamate-cystine transporter (SLC7A11) and provoked ferroptosis, which was corroborated by the depletion of glutathione, reduced nicotinamide adenine dinucleotide phosphate, and boost of lipid peroxides and malondialdehyde in SKOV3 cells. The current work integrated the “sequential amplification and inhibition of MCL-1 target” concept with combinational apoptosis and ferroptosis OC therapy, which might offer a new approach for managing resistant OC and other malignant diseases.
研究领域:
肿瘤学
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药学
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细胞生物学
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纳米技术
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DOI
高影响因子
RADIATION PHYSICS AND CHEMISTRY
·
2025
·
IF 3.3
Hanqin Weng,Baoying Zhang,Yizhi Chen,Shinichi Yamashita,Yusa Muroya,Hao Yu,Xinrui Zhang,Xiaofan Yang,Weiqun Shi
The ligand of 2,9-Diamide-1,10-phenanthroline (DAPhen), developed for group actinide separation in the past decade, showed excellent extraction and separation performance for various actinides from trivalent lanthanides. However, its radiolysis mechanism remains unclear, restricting its application. Mechanism for the radiolysis of three different DAPhen in 1-octanol was studied by pulse radiolysis, of which the results demonstrated that reductive degradation was the main route of their radiolysis in 1-octanol. Solvated electrons (e sol − ), and α-hydroxyoctyl radicals have been recognized as the most important transient species responsible for DAPhen radiolysis. Rate constants for the reactions of e sol − with N,N′ -diethyl- N,N′ -ditethyl-2,9-diamide-1,10-phenanthroline (Et–Et-DAPhen, EED), N,N′ -dioctyl- N,N′ -ditoctlyl-2,9-diamide-1,10-phenanthroline (Oct-Oct-DAPhen, OOD), and N,N′ -diethyl- N,N′ -ditolyl-2,9-diamide-1,10-phenanthroline (Et-Tol-DAPhen, ETD) were estimated to be 1.9 × 10 9 , 2.3 × 10 9 , and 1.5 × 10 9 L mol −1 s −1 , respectively. Those of α-hydroxyoctyl radicals with EED, OOD, and ETD were approximately 1.1 × 10 8 , 2.1 × 10 8 , and 2.7 × 10 8 L mol −1 s −1 , respectively. The studied DAPhen exhibited high reactivity with e sol − , and α-hydroxyoctyl radicals in 1-octanol, resulting in their vulnerability under irradiation. A versatile strategy was accordingly proposed to enhance their radiation stability based on the insights into transient processes of DAPhen radiolysis. If the HNO 3 -equilibrated organic phase was saturated with nitrous oxide (N 2 O), the reductive species responsible for DAPhen degradation would be scavenged. As a consequence, the radiation-resistance of DAPhen was significantly improved because the key elementary reaction of radiolysis was inhibited by the synergetic effect of N 2 O and HNO 3 .
研究领域:
放射化学
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配体设计
·
核化学与技术
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DOI
高影响因子
RADIOCHIMICA ACTA
·
2022
·Vol. 111
·期 1
·63-71
·
IF 2.083
Peng Liu,Zhuang Shen,Jiaxian Cheng,Zhen Han,Wenda Xu,Mingbo Ji,Fuqiu Ma
Due to the relative abundance, long half-life and high mobility of radioactive cesium (Cs), new adsorbents are urgently needed to treat Cs to ensure public health. In this study, a graphene oxide (GO) based adsorbent for Cs(I) adsorption was prepared by 1-aza-18-crown-6 ether modification. XRD, FT-IR, XPS and SEM results showed that the properties of 1-aza-18-crown 6 ether modified GO (18C6-GO) changed dramatically compared with that of raw graphite. The adsorption properties of 18C6-GO for Cs(I) were studied by batch static adsorption experiments. The results showed that the adsorption equilibrium time of 18C6-GO was 20 h. Kinetic study revealed that the adsorption rate of Cs(I) conformed to pseudo-second-order kinetic model. Langmuir adsorption isotherm simulation indicated that the adsorption arises at homogeneous adsorption sites on 18C6-GO. Therefore, crown ether modified GO may have implications for the treatment of wastewater.
研究领域:
环境科学
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材料科学
·
化学工程
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DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Hairui Huang,Hanyu Wu,Yizhou Zhang,Xunda Feng
Molecular-scale adsorption, catalysis, and separation demand nanoporous materials with high permeability, extensive surface areas, and pronounced nanoconfined effects. Fabricating polymeric particles with 3-D interwoven pores of ∼1 nm potentially addresses these needs. However, significant challenges remain in controlling their pore interconnectivity, uniformity, and achieving faceted particle shapes. Herein we present facile fabrication of polyhedral particles possessing interpenetrating 1 nm pores by suspension polymerization of double-gyroid (DG) liquid crystalline droplets. Mechanical stirring of the disordered phase at elevated temperatures, followed by undercooling, leads to the emulsification of DG droplets, as confirmed by synchrotron small-angle X-ray scattering (SAXS). UV-induced cross-linking of the DG droplets preserves the ordered network of 1 nm pores, as characterized by SAXS and microscopy. Intriguingly, due to the elasticity induced by the Ia3̅d periodicities, these particles adopt polyhedral shapes to avoid the elastic energy penalty associated with conventional sphericity. We demonstrate that these faceted particles, featuring 1 nm pores and efficient packing, enable rapid, size-exclusive adsorption and nanoconfined degradation of organic pollutants, driven by their 3-D permeability, high surface area, and enhanced nanoconfinement effects.
研究领域:
材料科学
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环境科学
·
化学工程
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF CATALYSIS
·
2025
·
IF 17.7
Shaolei Gao,Peng Lu,Liang Qi,Yingli Wang,Hua Li,Mao Ye,Valentin Valtchev,Alexis T. Bell,Zhongmin Liu
Methyl methoxyacetate (MMAc) and methyl formate (MF) can be produced directly by heterogeneous zeolite-catalyzed carbonylation and disproportionation of dimethoxymethane (DMM), with near 100% selectivity for each process. Despite continuous research efforts, the insight into the reaction mechanism and kinetics theory are still in their nascent stage. In this study, ZEO-1 material, a zeolite with up to now the largest cages comprising 16×16-MRs, 16×12-MRs, and 12×12-MRs, was explored for DMM carbonylation and disproportionation reactions. The rate of MMAc formation based on accessible Brönsted acid sites is 2.5 times higher for ZEO-1 (Si/Al = 21) relative to the previously investigated FAU (Si/Al = 15), indicating the positive effect of spatial separation of active sites in ZEO-1 on catalytic activity. A higher MF formation rate is also observed over ZEO-1 with lower activation energy (79.94 vs. 95.19 kJ/mol) compared with FAU (Si/Al = 30). Two types of active sites are proposed within ZEO-1 zeolite: Site 1 located in large cages formed by 16×16-MRs and 16×12-MRs, which is active predominantly for MMAc formation, and Site 2 located in smaller cages for methyl formate/dimethyl ether formation. Kinetics investigation of DMM carbonylation over ZEO-1 exhibit a first-order dependence on CO partial pressure and a slightly inverse-order dependence on DMM partial pressure. The DMM disproportionation is nearly first-order dependence on DMM partial pressure, while it reveals a strongly inverse dependence with increasing CO partial pressure. Furthermore, ZEO-1 exhibits good catalytic stability, and almost no deactivation is observed during the more than 70 h test with high carbonylation selectivity of above 89%, due to the well-enhanced diffusion property demonstrated by intelligent-gravimetric analysis.
研究领域:
化学工程
·
催化科学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Kaifang Wang,Danlin Chen,Jiali Tang,Zihao Hong,Zhongtai Zhu,Ziyi Yuan,Zhihong Lin,Yuqi Liu,Raphael Semiat,Xuezhong He
Membrane gas separation technologies have emerged as highly prospective avenues for the selective separation of CO 2 . In this research, a novel array of mixed-matrix membranes (MMMs) was engineered, integrating two-dimensional nanosheets of MXene, specifically Ti 3 C 2 T x , into polymers of intrinsic microporosity (PIMs) to selectively separate CO 2 from N 2 . The inclusion of Ti 3 C 2 T x nanosheets, rich in polar functional groups, significantly increases the affinity between CO 2 molecules and the fabricated MMMs. Additionally, the precise modulation of the molecular diffusion channels for CO 2 and N 2 is achievable due to the interlayer spacing between MXene layers (∼0.35 nm) within the PIM-1 matrix. This innovation is predicated on the synergistic effect of solution-diffusion and molecular sieving mechanisms that underpin the gas separation process, leading to a marked improvement in both selectivity for CO 2 over N 2 and the permeability of CO 2 . Among the series, the MMMs with a 0.5 wt% MXene display exceptional separation efficiency, achieving a CO 2 permeability of 12475.3 barrer and a CO 2 /N 2 selectivity of 32.7. Notably, all the PIM-1/MXene membranes prepared with different compositions in this study exceed the Robeson upper bound defined in 2019, indicating their substantial promise for CO 2 separation and capture in industrial applications.
研究领域:
材料科学
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环境工程
·
化学工程
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产品关联
DOI
高影响因子
Molecular Catalysis
·
2024
·
IF 3.9
Yuhao Yang,Minghui Tan,Hailun Geng,Xiujuan Gao,Yisheng Tan,Qingde Zhang
Oriented designing the microenvironment of catalytic sites is essential for understanding catalytic processes. In this work, EDTA was first grafted in UiO-66 and then chelated Rh to obtain an efficient catalyst for 1-hexene hydroformylation reaction. EDTA not only served as a chelation site for Rh immobilization in UiO-66, but also markedly influenced the structure and properties of Rh/UiO-66. The incorporation of EDTA reduced the micropore content and suppressed the formation of heavy products. Moreover, the grafted EDTA regulated the electronic state of Rh effectively to improve the CO and propene adsorption, thus constructing an appropriate microenvironment for hydroformylation. Therefore, EDTA-modified Rh/U-E catalyst exhibited higher aldehydes yield, TOF and stability than Rh/U without EDTA. The dual role of EDTA in anchoring Rh sites and modulating the microenvironment for hydroformylation provides more prospects for designing customized MOFs-based catalysts.
研究领域:
催化化学
·
材料科学
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产品关联
DOI
高影响因子
ACTA PHARMACOLOGICA SINICA
·
2025
·
IF 8.4
Xia Ling-yan,Yu Nai-rong,Huang Su-ling,Qu Hui,Qin Li,Zhao Qin-shi,Leng Ying
Non-alcoholic steatohepatitis (NASH) has emerged as a prevalent chronic liver disease with a huge unmet clinical need. A few studies have reported the beneficial effects of Poria cocos Wolf ( P. cocos ) extract on NASH mice, but the active components were still unknown. In this study we investigated the therapeutic effects of dehydrotrametenolic acid methyl ester (ZQS5029-1), a lanosterol-7,9(11)-diene triterpenes in P. cocos , in a high-fat diet plus CCl 4 induced murine NASH model and a GAN diet induced ob/ob murine NASH model. The NASH mice were treated with ZQS5029-1 (75 mg·kg –1 ·d –1 , i.g.) for 6 and 8 weeks, respectively. We showed that ZQS5029-1 treatment markedly relieved liver injury, inflammation and fibrosis in both the murine NASH models. We found that ZQS5029-1 treatment significantly suppressed hepatic NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome activation in both the NASH murine models, and blocked lipopolysaccharides (LPS)+adenosine 5’-triphosphate (ATP)/Nigericin-induced NLRP3 inflammasome activation in bone marrow-derived macrophages (BMDMs) and Kupffer cells in vitro. We demonstrated that ZQS5029-1 directly bound to the H236 residue of mouse Caspase-1, thereby inhibiting NLRP3 inflammasome activation. The effects of ZQS5029-1 on macrophage-hepatocyte/HSC crosstalk were analyzed using the supernatants from macrophages preconditioned with LPS + ATP introduced into hepatocytes and hepatic stellate cells (HSCs). We found that the conditioned medium from the BMDMs induced injury and death, as well as lipid accumulation in hepatocytes, and activation of HSCs; these effects were blocked by conditioned medium from BMDMs treated with ZQS5029-1. Moreover, the protective effects of ZQS5029-1 on hepatocytes and HSCs were eliminated by H236A-mutation of Caspase-1. We conclude that ZQS5029-1 is a promising lead compound for the treatment of NASH by inhibiting NLRP3 inflammasome activation through targeting Caspase-1 and regulating the macrophage-hepatocyte/HSC crosstalk.
研究领域:
药理学
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肝脏病学
·
免疫学
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DOI
PubMed
高影响因子
Communications Biology
·
2024
·Vol. 7
·期 1
·1-13
·
IF 5.2
Chen Song,Hu Zongren,Tang Jianbang,Zhu Haipeng,Zheng Yuhua,Xiao Jiedong,Xu Youhua,Wang Yao,Luo Yi,Mo Xiaoying,Wu Yalan,Guo Jianwen,Zhang Yongliang,Luo Huanhuan
High temperature and humidity in the environment are known to be associated with discomfort and disease, yet the underlying mechanisms remain unclear. We observed a decrease in plasma glucagon-like peptide-1 levels in response to high-temperature and humidity conditions. Through 16S rRNA gene sequencing, alterations in the gut microbiota composition were identified following exposure to high temperature and humidity conditions. Notably, changes in the gut microbiota have been implicated in bile acid synthesis. Further analysis revealed a decrease in lithocholic acid levels in high-temperature and humidity conditions. Subsequent in vitro experiments demonstrated that lithocholic acid increases glucagon-like peptide-1 secretion in NCI-H716 cells. Proteomic analysis indicated upregulation of farnesoid X receptor expression in the ileum. In vitro experiments revealed that the combination of lithocholic acid with farnesoid X receptor inhibitors resulted in a significant increase in GLP-1 levels compared to lithocholic acid alone. In this study, we elucidate the mechanism by which reduced lithocholic acid suppresses glucagon-like peptide 1 via farnesoid X receptor activation under high-temperature and humidity condition.
研究领域:
生理学
·
微生物学
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内分泌学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Linhui Feng,Heping Cui,Khizar Hayat,Xiaoming Zhang,Chi-Tang Ho
The Amadori rearrangement product (GR-ARP) derived from glutathione/ribose mixture (GSH/Rib) was prepared through spray drying characterized by short-term high temperature dehydration. The water activity of spray-dried samples containing GR-ARP decreased continuously with an intensified degree of dehydration. Lower water activity (from 0.23 to 0.13) and higher GR-ARP yield (from 46.56% to 64.02%) in the spray-dried products were found with the increase of inlet temperature, the slowdown of feed speed, and the alkalization of stock solution. Moreover, during the spray drying and formation of powders, the intensified dissipation of bound water to free water promoted intermolecular condensation that compensated bound water, favoring the directed conversion of GSH/Rib into GR-ARP. Compared to rotary evaporation at 80 °C, spray drying enabled a stronger restraint of amide cleavage and sulfhydryl oxidation in GSH, which reduced the yield of GSH-derived byproducts by 16.39%. Thus, GR-ARP was achieved a peak yield of 64.02% at an initial pH of 7.00 based on more efficient intermolecular condensation and controlled side reactions of GSH when the inlet temperature was set at 170 °C. The spray-dried sample containing richer GR-ARP and other flavor precursors showed a more similar flavor profile to the heated GSH/Rib mixture (complete Maillard reaction products) than other GR-ARP-related thermal processing samples, such as vacuum-dehydrated, GR-ARP, GR-ARP + GSH, and GR-ARP + Rib samples, revealing a great potential of the ARP mixture prepared by spray drying for flavor enhancement.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2024
·
IF 5.8
Haihan Huang,Jie Zheng,Kaiyue Wu,Jian Lin,Xin Lin,Quan Yao,Qianyu Fan,Dechao Wang,Yuanbo Huang,Jianchun Jiang,Zhifeng Zheng
Catalytic pyrolysis technology is one of the most environmental and efficient approach in converting biomass waste into high-value chemicals and fuels. In this study, the ex-situ catalytic pyrolysis of cellulose to produce furans and olefins was reported using various metal-loaded TS-1 as catalysts. The experimental results showed that the Au/TS-1 exhibited an exceptional catalytic activity in the production of furans and olefins, achieving a selectivity of 49.17 % and 40.65 %, respectively. Ni/TS-1 effectively promoted the formation of ethylene, yielding a selectivity of 97.49 % and a yield of 6.11 C-mol%. Under the optimized conditions: pyrolysis temperature of 550 ℃, catalytic temperature of 600 ℃, and catalyst to cellulose mass ratio of 20:1, the Au/TS-1 catalyst achieved a furans selectivity of 41.91 % with a yield of 11.94 C-mol%, while olefins selectivity and yield were 49.84 % and 18.99 C-mol%, respectively. At this condition, Au/TS-1 still has excellent catalytic activity after 10 cycles and has good catalytic effect towards different biomass feedstocks. Furthermore, the possible mechanism to produce furans and olefins was revealed. This research offers valuable insights for guiding the directed conversion of biomass into value-added chemicals, paving the way for a more sustainable future.
研究领域:
化学工程
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催化
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生物质转化
·
可再生能源
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）