BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
·
2013
·Vol. 17
·期 6
·1128-1134
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IF 1.277
Li Bin,Wang Lei,Su Lingqia,Chen Sheng,Li Zhaofeng,Chen Jian,Wu Jing
OmpA signal peptide mediated cgt gene from Paenibacillus macerans JFB05-01 was cloned and expressed in E. coli BL21 (DE3). The effects of glycine and Triton X-100 on extracellular production of α-cyclodextrin glycosyltransferase (α-CGTase) were investigated. When supplemented with Gly or Triton X-100 to the culture media individually, the secreted extracellular enzyme reached 32 or 33 U/mL at 48 h of cultivation, respectively. When supplemented with Gly and Triton X-100 together, the extracellular α-CGTase activity reached 48 U/mL after 48 h cultivation, which was 20-fold of the control group without any additives. Analysis of membrane permeability demonstrated that addition of glycine and Triton X-100 enhanced the permeability of both outer and inner membrane. The potential mechanism of the enhanced protein secretion was discussed.
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Journal of Food Measurement and Characterization
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2023
·
IF 3.4
Du Juan,Liu Kai,Liu Jialei,Zhao Dianbo,Bai Yanhong
Herein, a novel core-shell material Fe 3 O 4 @MIL-100(Fe) was synthesized for developing a novel LFIA by a two-step solvothermal method with good magnetic properties and high specific surface area. It was used as both magnetic separation and label agents in LFIA for the detection of Listeria monocytogenes . The enrichment of target bacteria can be achieved quickly and easily by direct adsorption of Fe 3 O 4 @MIL-100(Fe) on the bacteria and by the action of an applied magnetic field. It was beneficial in separation and concentration of target bacteria from food samples. The Fe 3 O 4 @MIL-100(Fe)-bacterial was then detected by the anti- Listeria monocytogenes antibody on the T line under the capillary action of the LFIA strip, resulting in the formation of a visible orange band. Under the optimal conditions, there was a good linear relationship between the intensity of the T line and the quantity of Listeria monocytogenes in the range of 10 5 -10 8 CFU mL − 1 , with a visual detection limit of 3.3 × 10 6 CFU mL − 1 . The strip showed strong specificity for L. monocytogenes , and the detection could be finished in 45 min, without the needing of pre-treatment. Thus, the proposed Fe 3 O 4 @MIL-100(Fe)-based strip was simple prepared, label-free, low cost, rapid and convenience.
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ACS Sustainable Chemistry & Engineering
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2022
·
IF 9.224
Jiabi Xu,Jiayi Peng,Xiangting Wang,Xiaohong Hou
Nanozymes have gradually become a research hotspot in the field of enzymology, benefiting from their low cost, high stability, and availability. To break through the limitation that conventional nanozymes exert peroxidase-like (POD-like) activity under only acidic conditions, we rationally designed Fe3O4@MIL-100(Fe) with excellent superparamagnetism and catalytic activity. An optimization of the microwave-assisted in situ synthesis conditions was carried out to obtain the highest POD-like activity of the nanozyme under nearly neutral conditions. Adenosine triphosphate (ATP) was appointed as an auxiliary regulator to further amplify the activity of the nanozyme Fe3O4@MIL-100(Fe). A series of behaviors related to enzymology and the mechanism of ATP-assisted enhancement were further explored. Consequently, a colorimetric sensing strategy was proposed for the one-step detection of cholesterol with a linear range of 0.05–0.4 mM and a limit of detection (LOD) of 1.3 μM (3.3 δ/S). The result of the present work implied that the established one-step platform could be successfully applied to the detection of cholesterol in actual samples. From the nanozyme synthesis to the colorimetric sensing process, the concepts of time savings and straightforwardness were always upheld and satisfactorily implemented. Most importantly, Fe3O4@MIL-100(Fe) could be a great potential biocatalyst candidate for the development of the corresponding commercial kits.
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JOURNAL OF HAZARDOUS MATERIALS
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2022
·
IF 14.224
Rongsheng Ning,Heliang Pang,Zhongsen Yan,Zhenyu Lu,Qiankun Wang,Zengling Wu,Wenxin Dai,Lingshan Liu,Zhongsheng Li,Gongduan Fan,Xianzhi Fu
The development of high efficient photocatalysts for antibiotics contamination in water remains a severe challenge. In this study, a novel step-scheme (S-scheme) photocatalytic heterojunction nanocomposites were fabricated from integrating AgCl nanoparticles on the MIL-100(Fe) octahedron surface through facile multi-stage stirring strategy. The S-scheme heterojunction structure in AgCl/MIL-100(Fe) (AM) nanocomposite provided a more rational utilization of electrons (e - ) and holes (h + ), accelerated the carrier transport at the junction interface, and enhanced the overall photocatalytic performance of nanomaterials . The visible-light-driven photocatalysts were used to degrade sulfamethazine (SMZ) which attained a high removal efficiency (99.9%). The reaction mechanisms of SMZ degradation in the AM photocatalytic system were explored by electron spin resonance (ESR) and active species capture experiments, which superoxide radical (•O 2 - ), hydroxyl radical (•OH), and h + performed as major roles. More importantly, the SMZ degradation pathway and toxicity assessment were proposed. There were four main pathways of SMZ degradation, including the processes of oxidation , hydroxylation, denitrification, and desulfonation. The toxicity of the final products in each pathway was lower than that of the parent according to the toxicity evaluation results. Therefore, this work might provide new insights into the environmentally-friendly photocatalytic processes of S-scheme AM nanocomposites for the efficient degradation of antibiotics pollutants.
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JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Jiang-Bo Huo,Guoce Yu
Efficient elimination of As(III) from drinking water and wastewater has been a challenge because of its neutral molecular form. To address this problem, a novel nanocomposite , mesoporous cerium oxide-anchored magnetic polyhedrons derived from MIL-100(Fe) was fabricated via a strategy combining impregnation and calcination . The resultant products (denoted as Fe 2 O 3 /CeO 2 -t) exhibited a unique octahedral nanostructure decorated by mesoporous cerium oxide . Surface modification of CeO 2 enhanced As(III) removal in comparison to unmodified Fe 2 O 3 . Particularly, Fe 2 O 3 /CeO 2 -4 h can reduce As(III) concentration from 180 to 10 µg/L within 20 min, which was almost 9 times faster than unmodified Fe 2 O 3 . The adsorption behavior conformed to the pseudo-second-order kinetic model ( R 2 = 0.9908) and the Freundlich isotherm model ( R 2 = 0.9943). The maximum adsorption capacity of As(III) by Fe 2 O 3 /CeO 2 -4 h was 68.25 mg/g, higher than those reported for similar adsorbents. Its enhanced removal mechanism can be attributed mainly to the mesoporous characteristics and oxidization ability of surface ceria. The composite can be separated from water by external magnets and easily regenerated. This study may offer a clue to the design of metal-organic framework-based composites as an alternative adsorbent for arsenite cleanup.
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Journal of Environmental Chemical Engineering
·
2020
·
IF 4.3
Shanshan Chen,Zhongyang Zang,Shusheng Zhang,Gangfeng Ouyang,Runping Han
Metal-organic framework and nano carbon materials were widely used in separation field in recent years. In this study, nanocomposite of MIL-100(Fe) and multi-walled carbon nanotubes (MIL-100(Fe)-CNTs) was prepared and characterized and its adsorption property towards Oxytetracycline (OTC) was presented. MIL-100(Fe)-CNTs exhibits higher adsorption capacity compared to MIL-100(Fe), making this composite as one of the favorable adsorbents. The adsorption capacity of MIL-100(Fe)-CNTs reached 429 mg·g −1 at 313 K. The langmuir model and Koble-Corrigan model were better to predict the equilibrium data while the pseudo-second-order kinetic model could well describe the kinetic process. The process was endothermic and spontaneous while the ion exchange was the main adsorption mechanism of OTC on MIL-100(Fe)-CNTs. The nanocomposite is promising to effectively remove OTC from solution.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
·
IF 3.232
Wenjun Zhou,Qiang Gao,Darui Liu,Chen Li,Shu Liu,Kaisheng Xia,Bo Han,Chenggang Zhou
A novel salicylaldehyde bis-Schiff-base probe decorated with imidazolium ionic liquid moieties at both ends (SAS-IMIs) was designed and facilely synthesized as a colorimetric/ratiometric sensor to visually detect Cu 2+ and Pd 2+ in pure aqueous media. Due to the positively charged characters of its head and tail, the SAS-IMIs exhibited high water solubility with a potential advantage in minimizing the self-aggregation. More importantly, simply by varying the solution pH, colorimetric/ratiometric sensing detection of individual metal ion (Cu 2+ or Pd 2+ ) was realized without any mutual interference. Subsequently, sensitive, selective, and differential detections for Cu 2+ (LOD: 0.080 μM) and Pd 2+ (LOD: 0.076 μM) in 100% aqueous solutions were achieved, which proved to be applicable for real water samples. Results from density functional theory (DFT) calculations unveiled the Cu 2+ /Pd 2+ -binding properties of SAS-IMIs, which were in accordance with the experimental observations. Furthermore, a SAS-IMIs-based solid phase sensor was fabricated, which manifested satisfactory detection abilities for Cu 2+ and Pd 2+ .
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 27
·期 30
·37839-37851
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IF 3.056
Xu Jun,Xu Jinmei,Jiang Shanqing,Cao Yu,Xu Kailin,Zhang Qiuya,Wang Liping
In this work, a novel visible light–driven heterostructure Ag 3 PO 4 /MIL-100(Fe) composite photocatalyst was successfully synthesized via facile chemical deposition method at room temperature. Especially when the mass ratio of Ag 3 PO 4 was 20% of MIL-100(Fe) (APM-2), it displayed the best photocatalytic performance, for which the degradation rate of tetracycline (TC) in conventional environment was 6.8 times higher than that of bare MIL-100(Fe). In addition, the effects of the initial concentration and pH of the solution on the degradation of tetracycline were also studied, and the results showed that the degradation of tetracycline was more favorable in a weakly alkaline environment. The excellent performance of Ag 3 PO 4 /MIL-100(Fe) composites was attributed to the fact that on the basis of having adequate photocatalytic active sites, modifying MIL-100(Fe) with an appropriate amount of Ag 3 PO 4 particles can more effectively separate photogenerated electron–hole pairs. Five cycles of experiments showed that APM-2 has good photostability. Lastly, it was proved through quenching experiments that •O 2 − , h + , and •OH all played corresponding roles in the degradation process, and a possible Z-scheme heterostructure photocatalytic degradation mechanism was proposed.
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Journal of Cleaner Production
·
2021
·
IF 9.297
Lizhen Guo,Yuqi Wang,Meng Wang,Hiba Shaghaleh,Yousef Alhaj Hamoud,Xu Xu,He Liu
With the severe shortage of water resources and the low utilization efficiency of fertilizer, slow-release fertilizer equipped with water-absorbing and water-retaining capacity has captured much attention. Herein, by integrating metal-organic frameworks (MOFs) with cellulose nanofibres (CNFs) and sodium alginate (SA), a novel cellulose-based hydrogel (MIL-100(Fe)@CNF-SA) was fabricated that acted as a carrier for urea. The resultant hydrogel was not only qualified with a high surface area of 129.17 m 2 /g but also affluent in the hydrophilic groups . In parallel with the good water-holding capacity of 55% when the loading of the resultant hydrogel was only 0.5 g, the complete loss of the water in the treated soil was successfully slowed down by nearly 20 d compared with the soil without treatment. Urea (1.47 g/g) could be captured in the composite hydrogel , wherein the slow release of urea was achieved due to the synergism of the highly porous structure of the hydrogel and the crystals with a high specific surface area. Moreover, the positive effect of the resultant hydrogel on the growth of wheat was indexed to the germination rate, number of tillers, photosynthetic rate and chlorophyll content of the crop, which verified their further application in irrigating farming.
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MOLECULES
·
2019
·Vol. 24
·期 20
·3718
·
IF 3.06
Mao-Long Chen,Shu-Yang Zhou,Zhou Xu,Li Ding,Yun-Hui Cheng
MIL-100(Fe, Cr) and MIL-101(Cr) were synthesized by the hydrothermal method and applied to the adsorptions of five aromatic amines from aqueous solutions. These three metal-organic frameworks (MOFs) were well characterized by powder X-ray diffraction (PXRD), scanning electron microscope (SEM), transmission electron microscope (TEM), thermogravimetric analysis (TGA) and surface area analysis. The adsorption mechanism of three MOFs and the effects of the structures of MOFs on the adsorption of aromatic amines were discussed. The results show that the cavity system and suitable hydrogen bond acceptor were important factors for the adsorption for five aromatic amines of aniline, 1-naphthalamine, o-toluidine, 2-amino-4-nitrotoluene and 2-nitroaniline: (a) the saturated adsorption capacity of aniline, 1-naphthylamine and o-toluidine on MIL-100(Fe) were 52.0, 53.4 and 49.6 mg/g, respectively, which can be attributed to the intermolecular hydrogen bond interaction and cavity system diffusion. (b) The adsorption capacity of 2-nitroaniline and 2-amino-4-nitrotoluene on MIL-101(Cr) were 54.3 and 25.0 mg/g, respectively, which can be attributed to the more suitable pore size of MIL-101(Cr) than that of MIL-100(Fe, Cr). The MOFs of MIL-100(Fe) and MIL-101(Cr) can be potential materials for removing aromatic amines from aqueous solutions.Keywords:metal-organic framework;adsorption;aromatic amines;aniline;1-naphthalamine;o-toluidine;2-amino-4-nitrotoluene;2-nitroaniline
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JOURNAL OF CHEMICAL THERMODYNAMICS
·
2014
·
IF 2.423
Yuanchao Pei,Yanjie Huang,Lin Li,Jianji Wang
In this paper, micro-emulsions composed of cholinium-based ionic liquids (ILs), octylphenol ethoxylate (Triton X-100) and water were prepared. These ternary systems were found to be stable over 12 months at room temperature. Their phase behaviour was investigated by using cloud titrations, and their microstructures were characterised by means of cyclic voltammetry and electrical conductance measurements at T = 298.15 K. It was shown that the micro-emulsions exhibited IL-in-water, bi-continuous and water-in-IL microstructures. Dynamic light scattering data suggest that Triton X-100 forms micelles in water, which were swelled by the ILs added. Droplets with the size about 20 nm were formed in these IL-based micro-emulsions, and the droplet size increased with the increase of the IL concentrations. These IL-based micro-emulsions may have potential in drug delivery, chemical reactions and nanomaterial preparation as a new type of nanoreactors.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2017
·
IF 2.536
Nan Gao,Pengcheng Huang,Fangying Wu
In this study, we have developed a method for rapid, highly efficient and selective detection of melamine . The negatively charged citrate ions form an electrostatic layer on gold nanoparticles (AuNPs) and keep the NPs dispersed and stable. When citrate-capped AuNPs were further modified with Triton X-100, it stabilized the AuNPs against the conditions of high ionic strength and a broad pH range. However, the addition of melamine caused the destabilization and aggregation of NPs. This may be attributed to the interaction between melamine and the AuNPs through the ligand exchange with citrate ions on the surface of AuNPs leading Triton X-100 to be removed. As a result, the AuNPs were unstable, resulting in the aggregation. The aggregation induced a wine red-to-blue color change, and a new absorption peak around 630 nm appeared. Triton X-100-AuNPs could selectively detect melamine at the concentration as low as 5.1 nM. This probe was successfully applied to detect melamine in milk. Furthermore, paper-based quantitative detection system using this colorimetric probe was also demonstrated by integrating with a smartphone.
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