INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2022
·Vol. 57
·期 12
·7917-7928
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IF 3.612
Yu Liu,Zheming Wang,Yizhen Huang,Kunihiko Konno,Tianjian Tong,Chenxu Yu,Beiwei Zhu,Xiuping Dong
Summary The dark muscle of yellowtail kingfish is regularly consumed as fresh sashimi alongside with the ordinary muscle. Thus, in this study, three types of ATPase activities, chymotryptic digestion patterns as well as thermal unfolding of various myosin domains were determined to reveal the denaturation characterisation of myosin in dark muscle and compare it with normal muscle. The thermal inactivation rate of dark muscle myosin Ca 2+ -ATPase at 30°C (7.3 × 10 −4 , sec −1 ) was significantly ( P < 0.05) greater than that of ordinary muscle (5.9 × 10 −4 , sec −1 ), while dark muscle myosin became more stable than ordinary muscle one once bound with F-actin. On the other hand, the rod region especially light meromyosin region of the dark muscle myosin was much more stable than ordinary myosin. These results characterised that myosin in the dark muscle of yellowtail kingfish is constituted with less stable head and more stable rod regions than those in the ordinary muscle.
研究领域:
生物化学
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肌肉生理学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
·
IF 4.6
Lingchen Wang,Yue Huang,Linlin Sun,Xiaojuan Gong,Chuan Dong,Shaomin Shuang
Herein, a novel rapid, specific, and sensitive probe for adenosine triphosphate (ATP) detection is developed. We have pioneered the integration of split ATP aptamers with aggregation-induced emission (AIE) via chemical binding to obtain a novel ATP detection probe (TPE probe). Upon the binding of the ATP split aptamer to the target, the intermolecular distance of the AIE molecules decreases, restricting their spatial rotation, which leads to an enhancement of the fluorescence signal, thereby enabling the detection of ATP. This unique design provides the probe with both target-specific recognition capability of the aptamer and sensitive signal-reporting function. Under the optimized experimental conditions, the probe exhibits a linearity range from 1-1000 nM with limit of detection 0.14 nM, displaying a comparative advantage over previously reported methods in terms of sensitivity and selectivity. Additionally, this method demonstrated satisfactory performance for ATP detection in beer samples, with recoveries between 97.5 % to 104.6 %. More importantly, the current research is expected to provide an effective monitoring method for beer quality control.
研究领域:
生物化学
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分析化学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Ruiyang Yu,Hui Qiao,Gengcheng Liao,Zongyu Huang,Qiaoliang Bao,Xiang Qi
Here, a photoelectrochemical (PEC) photodetector with good flexibility and high photoresponsivity was successfully fabricated in a vertical structure, where the MXene (Ti2CTx) nanosheet and carbon black electrode were separated by adenosine triphosphate/nicotinamide adenine dinucleotide phosphate (ATP/NADPH)-incorporated solid-state electrolyte. The photocurrent and photoresponsivity can reach 1.84 μA/cm2 and 8.89 μA/W, respectively, under a light intensity of 90 mW/cm2 at a bias potential of 0.6 V, which are approximately 2.3 times those of Ti2CTx nanosheets. The addition of ATP and NADPH to the electrolyte also leads to a large decrease of the rise time from 0.76 to 0.26 s. Furthermore, the photodetector can continue to function and maintain stability under 45° bending and after 500 cycles of bending, indicating a robust device structure and great flexibility. The performance enhancement of the PEC photodetector can be attributed to the synergistic effect of electrolyte additives on Ti2CTx nanosheets, where ATP and NADPH greatly enhance the circulation and utilization of photogenerated carriers. This work suggests that the incorporation of chloroplast-inspired carrier circulation with two-dimensional nanosheets could achieve efficient light-current conversion, providing a new strategy to improve the performance of PEC-type photodetectors.
研究领域:
材料科学
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光电器件
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生物启发设计
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DOI
PubMed
高影响因子
ACS Nano
·
2017
·
IF 13.942
Youqian Xu,Jinbo Fei,Guangle Li,Tingting Yuan,Junbai Li
Molecule assembly and functionalization of protocells have achieved a great success. However, the yield efficiency of photophosphorylation in the present cell-like systems is limited. Herein, inspired by natural photobacteria, we construct a protocell membrane reconstituting motor protein for highly efficient light-mediated adenosine triphosphate (ATP) synthesis through a layer-by-layer technique. The assembled membrane, compartmentally integrating photoacid generator, proton conductor, and ATP synthase, possesses excellent transparency, fast proton production, and quick proton transportation. Remarkably, these favorable features permit the formation of a large proton gradient in a confined region to drive ATP synthase to produce ATP with high efficiency (873 ATP s–1). It is the highest among the existing artificial photophosphorylation systems. Such a biomimetic system provides a bioenergy-supplying scenario for early photosynthetic life and holds promise in remotely controlled ATP-consumed biosensors, biocatalysts, and biodevices.
研究领域:
生物化学
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生物物理学
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合成生物学
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仿生系统
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Zhuoheng Zhong,Shengzhi Liu,Songling Han,Yaohan Li,Minglei Tao,Amin Liu,Qiang He,Sixue Chen,Criag Dufresne,Wei Zhu,Jingkui Tian
Indole alkaloids from Catharanthus roseus (L.) G. Don were valued for their wide spectrum of pharmaceutical effects. Their biosynthesis is modulated by various abiotic factors including ultraviolet B radiation which induced ATP production and led to accumulation of indole alkaloids. To investigate the regulatory mechanism of secondary metabolism in the leaf mitochondria of C. roseus under ultraviolet B radiation, combinatory study of proteomic and metabolomic analyses of C. roseus was performed. ATP export in the leaves of C. roseus increased under UVB radiation, which required mitochondrial ATP synthase. Proteins related to mitochondrial complexes II/IV and their gene expression levels increased, and those related to mitochondrial complex I decreased. Proteins related to the methylerythritol phosphate pathway, especially geranylgeranyl pyrophosphate synthase, increased under ultraviolet B radiation. Metabolites whose levels changed were mainly alkaloids, organic acids, carbohydrates, phenylpropanoids, and fatty acids, while eight indole alkaloids increased. Integrative analysis of omics data indicated that the metabolism of glutamate and tyrosine was downregulated. These results suggest that ultraviolet B radiation induces dynamic changes in mitochondria in C. roseus , which guarantees ATP production, regulates the flux of the methylerythritol phosphate pathway to the biosynthesis of monoterpene moieties, and leads to accumulation of various indole alkaloids.
研究领域:
植物生物化学
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分子生物学
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药理学
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环境科学
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DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
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2024
·
IF 2.7
Bo He,Jianuo Ma,Luobing Xiang,Han Wang,Xi Wang,Chunwang Yi
As a representative of dope-dye, the color masterbatch method has been widely applied in modern dyeing processes, while it has difficulties in solving the dispersibility of inorganic pigments color master batch in the polymer matrix. In this work, attapulgite (ATP) was used as the adsorption carrier of inorganic pigments to prepare color-dyed attapulgite (CD-ATP). The selected environmentally friendly inorganic pigments were well adsorbed by ATP in KOH aqueous solution, and could stably be monodispersed in KOH aqueous solution for more than 168 h. Moreover, the KOH aqueous solution could be reused repeatedly, thereby, a sustainable method to prepare novel CD-ATP was realized. By blending the as-prepared CD-ATP with polyamide-6 (PA6), a series of dope-dyed colorful PA6 composites was prepared. Scanning electron microscope (SEM) results indicated that the CD-ATP was still monodispersed in the PA6 matrix, and endowed PA6 with a vivid color. Further studies show that the adding of CD-ATP had little influence on the properties of PA6, but significantly reduced the water adsorption. This novel color-dyed ATP has potential implications in making paper-making blankets and is highly expected to have more extensive applications in many fields of life.
研究领域:
材料科学
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染色技术
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2022
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IF 10.383
Xiaoyan He,Nan Sun,Hui Jia,Miaomiao Hou,Zheping Tan,Xiaoquan Lu
As an energy supplier, ATP plays an important role in various life activities, and there is an urgent need to develop an effective means of detecting ATP. However, the traditional sensors face serious nonspecific adsorption. In this work, an antifouling electrochemical biosensor based on the interpenetrating network of Y-DNA scaffold and polyaniline hydrogel was designed for ATP detection. The polyaniline hydrogel was conducive to the transport of electrons and ions, the structure of Y-DNA cross-linked by ATP aptamers in the polyaniline hydrogel achieved the effect of signal amplification. Super hydrophilic cellulose nanocrystals (CNCs) and zwitterion polypeptide sequence (Pep) were doped to play a synergistic antifouling effect. The hydrogel sensor we have built has a wide linear range of 0.1 pM–1 μM for ATP detection and a low detection limit of 0.025 pM (S/N = 3). For ATP detection in actual serum samples, the recovery of this sensor was 99.5%–106%, and the relative standard deviation was 0.4%–2.88%. It is proven that the sensor has good ATP detection performance, and it will provide a certain reference value for the detection of other biological small molecules.
研究领域:
分析化学
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生物化学
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材料科学
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生物传感器
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DOI
PubMed
高影响因子
NEUROCHEMICAL RESEARCH
·
2026
·Vol. 51
·期 4
·232
·
IF 4.5
Zhang Xinfeng,Lian Zhuo,Lei Sijie,Yi Tanhui,Zhao Shuyin,Liu Mingxiang,Gui Yiling,Zhu Chengyi,Xie Qian,Ma Rong
The selection of appropriate in vitro inflammatory models is critical for mechanistic studies of neuroinflammation and the screening of anti-inflammatory therapeutics. Lipopolysaccharide (LPS) combined with interferon-γ (IFN-γ) and LPS combined with adenosine triphosphate (ATP) are widely used to induce microglial inflammation; however, their distinct phenotypic characteristics and optimal applications have not been systematically compared. In this study, we combined transcriptomic profiling with functional assays to compare these two inflammatory paradigms in BV2 microglia. Our results showed that both models activated the chemokine–cytokine inflammatory network and induced the production of inflammatory mediators and chemokines, but exhibited distinct molecular and functional biases. LPS/IFN-γ stimulation preferentially upregulated genes such as Lcn2 , Ccl2 , Upp1 , and Pim2 , which are associated with innate immune activation, chemokine-mediated inflammatory responses, and cell survival signaling, and was accompanied by marked increases in NO and TNF-α production. In contrast, the LPS/ATP model preferentially upregulated genes associated with ECM/cell adhesion, inflammatory and cellular homeostatic regulation, oxidative stress, and calcium-dependent secretion, including Thbs1 , Thbd , Srxn1 , Syt7 , and Procr . Functionally, this model exhibited more pronounced IL-1β maturation and release, relatively lower NO production, and greater mitochondrial dysfunction and cell injury. Pharmacological inhibition of NLRP3 with MCC950 significantly attenuated ATP-induced IL-1β maturation, supporting the involvement of the NLRP3 inflammasome in the LPS/ATP model. Overall, the LPS/IFN-γ model is more suitable for investigating the NO/iNOS–TNF-α axis, IFN-γ-mediated synergistic pro-inflammatory responses, and classical pro-inflammatory activation, as well as for screening anti-inflammatory agents. In contrast, the LPS/ATP model is more appropriate for studying IL-1β maturation and release, NLRP3 inflammasome activation, mitochondrial damage, and inflammation-related cell death, as well as for screening inflammasome inhibitors. Our findings provide an empirical framework for rational model selection in microglial inflammation research.
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高影响因子
Synthetic and Systems Biotechnology
·
2025
·
IF 4.4
Yue Chen,Linbo Gou,Di Liu,Shengfang Wu,Xiuwen Zhou,Tai-Ping Fan,Long Wang,Yujie Cai
(S)-(+)-linalool, a valuable acyclic monoterpenol secondary metabolite of plants, finds extensive applications in the food, flavor and fragrance, pharmaceutical, and daily chemical industries. Microbial synthesis offers two pathways for its production, among which the mevalonate (MVA) pathway derived from halophilic archaea is more widely employed. However, the traditional Haloarchaea-type MVA pathway relies on bifunctional enzyme catalysis, consuming 3 molecules of ATP. Moreover, the catalytic activity of natural linalool synthase (LIS) is generally low, failing to meet industrial requirements. Additionally, linalool exhibits significant toxicity to microbial hosts, thereby limiting the production capacity of conventional chassis microorganisms. To address these bottlenecks, this study implemented systematic optimizations: Firstly, the Archaeal mevalonate pathway was reconstructed by replacing the original bifunctional enzyme with two monofunctional enzymes, successfully reducing ATP consumption to 2 molecules. Secondly, through directed screening and rational design, a high-activity linalool synthase mutant, CsMLIS I331V/I444L , derived from Coriandrum sativum , was obtained. Furthermore, an enzyme fusion strategy was adopted, involving the introduction of a long flexible linker between key genes, which significantly enhanced catalytic efficiency. Finally, S . marcescens HBQA7Δ sIaAB-pyc , a strain previously screened in our laboratory with broad-spectrum tolerance to terpenoids, was selected as the novel chassis cell. Collectively, these efforts resulted in the construction of a microbial cell factory for the efficient synthesis of (S)-(+)-linalool, laying a solid foundation for industrial-scale production.
研究领域:
代谢工程
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合成生物学
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微生物生物技术
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酶工程
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工业生物技术
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jingzhe Zang,Yingyu Zhang,Xianwei Zhang,Mengxin Zhang,Jing Gao,Kangbo Liu,Ying Xu,Jie Zhang,Peng Wang,Wancun Zhang
The abnormal localization of mitochondrial adenosine triphosphate (ATP) is closely linked to tumor proliferation, migration, and drug resistance. While ATP is widely distributed in cells, such as in the cytoplasm, nucleus, organelles, and cell membranes. Consequently, the development of strategies with precise localization control for in situ imaging of mitochondrial ATP poses a significant challenge. Therefore, an apurinic/apyrimidinic endonuclease 1 (APE1) translocation-mediated domino-type DNA nanomachine (C-ATP-tFNA) was developed, which can target mitochondria by using the mitochondria-specific localization of cytochrome c (cyt c) as the targeting agent. The interaction between S5-hairpin 1 (S5-HP1) of C-ATP-tFNA and cyt c induces a conformational change in S5-HP1 to HP1, thereby exposing the AP site for cleavage by mitochondrial APE1 and releasing a single-strand cyclic DNA sequence (cyclic sequence), which leads to the first configurational change of C-ATP-tFNA. Then, the cyclic sequence can be hybridized with hairpin 2 (HP2) of C-ATP-tFNA in a circular mode, leading to a second cleavage by APE1 in a domino effect and thus causing the second configuration change of C-ATP-tFNA. Finally, upon cleavage of the AP site on HP2 by APE1, the ATP aptamer on HP2 is activated and gains the ability to bind ATP, thus generating a fluorescence signal in the presence of ATP. The experimental results of this study demonstrated that C-ATP-tFNA can achieve highly specificity and sensitivity in vivo imaging of mitochondrial ATP in tumor and inflammatory cells. In particular, C-ATP-tFNA can monitor drug resistance in neuroblastoma (NB) in vivo , offering a novel and reliable approach for the surveillance of NB drug resistance.
研究领域:
生物化学
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分子生物学
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纳米技术
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癌症生物学
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药理学
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生物医学成像
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产品关联
DOI
高影响因子
Journal of Applied Biomaterials & Functional Materials
·
2025
·
IF 3.1
Linxiang Zhang,Ting Dai,Xiaoyu Wu,Bin Yi,Hongbin Zhao
Guided tissue regeneration/guided bone regeneration membranes (GTR/GBR) are widely used to repair damaged bone, manufactured using various materials, including synthetic polymers, natural polymers, metals, and inorganic compounds. Our previous study showed that nano-attapulgite (nano-ATP) composite membranes could significantly increase bone regeneration. Here, the porous nano-ATP-incorporated CH membranes were fabricated via the technique of freeze gelation (FG). The property and thermal stability of membranes were tested by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA). Cell proliferation and morphology on the membranes were also studied. In vivo Micro-CT and histological analyses were used to evaluate new bone formation in rat cranium defect models. The results indicated that with increasing ATP content, the water absorption, porosity, swelling rate, and degradation rate of chitosan membranes significantly decreased; conversely, the thermal weight loss and mechanical strength exhibited a marked increase. Culture of mouse mesenchymal cells (D1) showed that ATP-incorporated membranes increased cell proliferation and matrix deposition. Furthermore, the results from Micro-CT and histological staining demonstrated that ATP-incorporated membranes could increase new bone formation. These results suggest that this novel nano-ATP incorporated membrane has great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.
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DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2020
·
IF 3.306
Qian Qiu,Ru-Ru Gao,Aming Xie,Yingzhi Jiao,Wei Dong
Content of adenosine triphosphate (ATP) is an essential index in the development of various diseases and even some malignant tumors. Biosensing for ATP with high sensitivity and accuracy is of fundamental importance in both biological research and medical diagnostics. Here we report a ratiometric fluorescent sensor for ATP based on multi-color silver nanoclusters (Ag NCs). This nanosystem contains two core components: a hairpin DNA integrated with an ATP aptamer, an anchor sequence and a special template segment for synthesis of green-emitting Ag NCs; a single-stranded DNA (ssDNA) which is incompletely complementary with the anchor sequence to be a duplex scaffold for red-emitting Ag NCs. Once exposed to the target, formation of the ATP/aptamer complexes opens the hairpin structure and releases the anchor sequence to hybridize with the ssDNA, accompanied by the “turn-off” of the green fluorescence and “turn-on” of the red fluorescence. By measuring the fluorescence intensity ratio of the two signals, we successfully establish a ratiometric sensor which exhibits sensitive and accurate assay performance toward ATP, with the limit of detection (LOD) as low as 0.38 μM. Meanwhile, this ratiometric sensor shows stable recovery in real sample assay, indicating their potential promise in practical applications.
研究领域:
生物传感
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医学诊断
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分子生物学
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DOI
高影响因子
危险品化学品经营许可证（带存储）