MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Linlin Chen,Songyao Wu,Wei Li,Ming Zhang,Xiao Yu,Jiaqi Song,Dong Liang,Jiaxin Zhang
Organophosphorus pesticides (OPs) are one of the most widely used pesticides in agricultural production, which can cause accumulation of acetylcholine, overstimulation of synaptic receptors, and ultimately lead to neurological damage. Based on the activity of hybrid lipase to catalyze the hydrolysis of OPs to produce P-NP and the inhibition of its ability to hydrolyze P-NPP by OPs, a dual assay system has been developed, which enables the detection of OPs with high sensitivity and low cost. Meanwhile, a hybridization lipase (BCL@Zn-hNF) with high catalytic activity and large surface area was prepared, and a cascaded sensor simulating peroxidase activity (BCL@Zn-hNF/AuNPs/Au) was established and used for electrochemical biosensor detection of OPs residues. In addition, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) results show that BCL@Zn-hNF consists of interlaced lamellar structures. Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD), X-ray Photoelectron Spectroscopy (XPS), and particle size analysis confirm the presence of phosphates. The hybridization process did not significantly affect its crystal morphology with good stability and dispersion. These results confirm the successful preparation of BCL@Zn-hNF. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) verified that adding BCL@Zn-hNF increased the sensor's catalytic rate. The sensor exhibited good sensitivity (5.182 × 10 8 A M −1 cm −2 ), a wide linear range (3.80 × 10 −8 –4.56 × 10 −5 M), and a low detection limit (1.03 × 10 −9 M) for the indirect detection of OPs compared with the direct detection. Meanwhile, it has good reproducibility, stability, and immunity to interference in the detection of OPs, and good recovery for the detection of MP residues in real samples.
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高影响因子
FOOD CONTROL
·
2025
·
IF 6.3
Xiaoyong Hu,Yuhuan Qin,Jiahui Zhao,Joseph Brake,Shuangbo Zhang,Wenxue Sun,Jiang Bian,Xiaobin Wu
The extensive use of chromium in various industrial processes may cause toxic effects in organisms due to its accumulation in the environment and foods. Traditional methods for chromium detection are time-consuming and infeasible for on-site usage. It is urgent to build a rapid, real-time and economical method for determining chromium to ensure human health and environmental safety. Here we developed an optimized colorimetric method for rapid detection of Cr 3+ ion based on the specific chromium chelator 2′3-dimercaptosuccinic acid (DMSA) which was screened by a novel biological method. Chemical methods further verified the specificity of DMSA for chromium. Both in vitro and in vivo experiments demonstrated that DMSA is safe for utilization. DMSA was complexed with gold nanoparticles (AuNPs) to increase the specificity and sensitivity for Cr 3+ . The optimized DMSA-AuNPs reaction achieved a color change from wine red to blue upon adding Cr 3+ at less than 5 μM. The naked-eye visual detection limit was 0.5 μM with a total reaction time of less than 1 min. The linear range of this colorimetric sensor was 0.5–5 μM and the detection limit reached 23 nM. The Cr 3+ detection by DMSA-AuNPs across several food samples including rice, soybean, milk, grape, peach and cabbage was consistent with those determined by inductively coupled plasma-mass spectrometry. Its detection results in 6 food samples (rice, soybean, milk, etc.) are highly consistent with ICP-MS (correlation coefficient R 2 > 0.99), and the entire detection process can be completed within 1 min. Our study provides an original and practical method for rapid, safe, on-site and cost-effective chromium monitoring of water, foods and the environment.
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高影响因子
SENSORS
·
2025
·Vol. 25
·期 24
·7462
·
IF 3.5
Yuanyue Lu,Yong Mei,Yingying Gu,Ye Tao,Yuhan Yang,Jiao Yu,Yang Zhang,Lin Liu,Xin Li
An electrochemical immunosensor was fabricated to identify CA15-3, a biomarker for breast cancer (BC). A composite sensor substrate made of “zeolitic imidazolate framework-8” (ZIF-8) and “reduced graphene oxide” (rGO) was chosen and its conductivity was further improved by the addition of chitosan (CS)-doped gold nanoparticles (AuNPs). The CS@AuNPs are able to conjugate with antibodies via the strong Au-S interaction, which offers multiple active sites for antibody immobilization and enhances the sensor performance. This immunosensor is capable of ultrasensitive detection of CA15-3 by specific antigen–antibody –interactions. In healthy people, normal serum CA15-3 is up to 25 U/mL. Under optimized experimental conditions, the alteration in the signal intensity measured by the sensor was related to the CA15-3 activity. The quantitative relationship was linear over 0.001–400 U/mL with a limit of detection (LOD) of 0.0031 U/mL at a “signal-to-noise ratio” (S/N) of 3 and a “correlation coefficient” (r2) of 0.9983. The developed immunosensor showed great accuracy, stability, and selectivity, and was able to detect CA15-3 in human serum samples. These results validate its potential as a reliable analytical platform for BC diagnosis and early clinical screening.
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PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Lihua Li,Shengpeng Zhang,Lizhen Yu,Wenzhi Zhang,Yan Wei,Dexiang Feng
A sensitive immunosensor for quantifying prostate specific antigen (PSA) had been described. In the design, gold nanoparticles decorated thionine-functionalized carbon nanospheres (CNSs/Thi@AuNPs) for immobilization of primary antibody (Ab 1 ) as an electrochemical sensing platform. Silicon dioxide nanospheres (SiO 2 NSs), with large surface area and good biocompatible, were loaded with horseradish peroxidase (HRP) and horseradish peroxidase labeled secondary antibodies (HRP-Ab 2 ). In the presence of PSA, a sandwich type immunocomplex from Ab 1 -Ag-Ab 2 had been formed. Differential pulse voltmmetry (DPV) was employed to obtain the response signal of immunosensor. Under the suitable experimental conditions, the changes of current intensity were linear with the logarithm of PSA concentration from 0.1 pg·mL -1 to 100 ng·mL -1 , and the detection limit was 0.025 pg·mL -1 (S/N=3). Moreover, the immunosensor possessed satisfactory specificity, repeatability and stability, suggesting that it had potential application value in the detection of tumor markers.
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高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2025
·Vol. 172
·期 4
·047501
·
IF 3.3
Yaping Zhang,Gao Si,Yilong Wang,Zhendong Wang,Zutian Li,Haiyan Wei,Huaixia Yang,Fuchun Si,Xiaofei Li
The sensitive detection of down-regulator of transcription 1 (DR1) provides important information for early noninvasive diagnosis and treatment of Hashimoto thyroiditis (HT). In this study, a label-free electrochemical biosensor for DR1 detection was constructed based on electrodeposited gold nanoparticles (AuNPs) and poly(lactic-co-glycolic acid) (PLGA). The AuNPs were first modified to the electrode surface by electrodeposition to increase the specific surface area of the electrode, thus loading more target molecules to increase the sensitivity of the sensor. Then, a sandwich structure was formed on the electrode surface based on the specific recognition of antigen and antibody. PLGA was fixed on the electrode surface by binding with antibody 2 (Ab2) through amide bond to further improve the sensitivity of the sensor. Induced by the dual signal amplification, the sensor has a linear detection range of 50 fg·ml−1 to 50 ng·ml−1, with a detection limit as low as 19.4 fg·ml−1. Moreover, the sensor has good selectivity, specificity, and anti-interference capability, which holds great potential for the early diagnosis of HT and analysis of other disease markers.
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高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Qianlong Wang,Lancong Liu,Xiaoyi Chen,Tiantian Wang,Hua Zhou,Hui Huang,Linsen Qing,Pei Luo
Urine is the most appropriate body fluid for analysis because it is easily and less-invasively obtained than blood; thus, urinary miRNAs can better represent the local stage of the disease and might grow up to be a new class of noninvasive biomarkers of postmyocardial infarction (MI). Monofunctionalized Au nanoparticles (AuNPs) with only one selective DNA at a specific location are more promising in nanotechnology. This study developed a urinary miRNA ultratrace detection strategy based on single-target DNA-functionalized AuNPs for the noninvasive prognosis of post-MI. The AuNPs were designed with only single-stranded biotinylated DNA complementary to the target miRNA through a ratio-optimized stoichiometric method for the first time. Combined with the duplex specific nuclease-assisted target recycling amplification, the single-target DNA-functionalized AuNPs for the first time were used in inductively coupled plasma–mass spectrometry for the determination of urinary miRNA with high sensitivity. After optimizing the reaction conditions, a linear detection range between 1 fM and 10 pM for miR-155 and a detection limit of 0.47 fM were obtained. Finally, the target miR-155 in urine samples collected from MI rats was quantified and the level of miR-155 in MI groups was 30 times higher than in the control groups. The results suggest that urinary miR-155 could be a novel biomarker for the noninvasive diagnosis of MI.
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DOI
PubMed
高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Yao Yu,Yansong Li,Qi Zhang,Yonghong Zha,Shiying Lu,Yong Yang,Peiwu Li,Yu Zhou
Fumonisin B1 (FB1) is one of the most toxic and widely distributed subtypes of the 20 fumonisins, which has attracted much attention in food and feed safety. In this study, a convenient colorimetric immunoassay was proposed for the detection of FB1 based on Mn 2+ -mediated aggregation of gold nanoparticles (AuNPs). After hydrolyzing of ascorbic acid 2-phosphate (AAP) by alkaline phosphatase (ALP), the generated ascorbic acid (AA) decomposes brown MnO 2 shell to Mn 2+ , which growth onto AuNPs and inducing the aggregation of AuNPs. The color change of the solution induced by aggregating of AuNPs can be read by smartphone to avoid subjective differences. The limit of detection (LOD) of this assay was 0.15 ng/mL and the limit of quantification (LOQ) was 0.50 ng/mL. The method has not cross-reaction with other mycotoxins . The average recoveries of FB1 in the maize flour samples ranged from 86.4 % to 110.9 %, which met the food and Drug Administration (FDA) restrictions on FBS in agricultural products. The colorimetric immunoassay can be potentially used for mycotoxin-related food safety monitoring.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Ningyi Chen,Hongyu Liu,Yujie Zhang,Zhuangwei Zhou,Wenpei Fan,Guocan Yu,Zheyu Shen,Aiguo Wu
A new colorimetric sensor based on citrate-stabilized AuNPs was proposed for the rapid pesticide residue detection of both terbuthylazine (TBA) and dimethoate (DMT) with ultra-sensitivities and high selectivities. The detection mechanisms have been verified via FT-IR, UV–vis spectra, Zeta Potential, TEM and DLS. Under the optimized experimental conditions, 30 kinds of potential environmental pollutants have no interference on the TBA or DMT detection indicating the high selectivities of our AuNP-based colorimetric sensor. The limit of detections (LODs) of TBA and DMT by eye vision are respectively 0.3 μM and 20 nM, and those based on calculation (3σ/S) are 0.02 μM and 6.2 nM, respectively. The minimum detectable concentrations of TBA or DMT are much lower than the maximum residue limit (MRL) regulated by the governments of EU and China. The linear relationships of the UV–vis spectrometry demonstrate that our AuNP-based colorimetric sensor can be used for the quantitative analysis of TBA in the range of 0.1–0.9 μM, and DMT in the range of 1–40 nM. Finally, our AuNP-based colorimetric sensor is also verified to have a good practical applicability for TBA or DMT detection in the real environmental samples.
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高影响因子
Biosensors-Basel
·
2026
·Vol. 16
·期 2
·103
·
IF 6.2
Luwei Chai,Yarong Wang,Shuang Jiang,Xue Wang,Yong Xie,Tao Le
Sulfamethazine (SMZ) is widely used in livestock production, and its residues can enter water and soil environments, posing potential risks to human health and ecosystems. This study focuses on environmental samples and constructs an AuNP-based colorimetric aptasensor using the SMZ1S aptamer for the rapid visual detection of SMZ. Under optimized conditions, the aptasensor showed a wide linear range from 0.05 to 0.4 µg/mL and a limit of detection of 0.039 µg/mL. Molecular dynamics simulations have demonstrated that the aptamer’s binding to SMZ is stable, providing a theoretical basis for the high selectivity of the aptasensor. Spike-and-recovery experiments yielded recoveries of 87.3–105.5%, 88.6–102.8%, and 87.5–103.4% for SMZ in lake water, tap water, and soil samples, respectively, with relative standard deviations of 5.9–8.3%, 8.0–10.6%, and 4.8–9.6%, showing good agreement with high-performance liquid chromatography (HPLC) results (R2≥ 0.981). Overall, the proposed aptasensor provides a simple and effective approach for rapid detection of SMZ in environmental samples.
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PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Zihan Kou,Jiameng Li,Linlin Li,Qianlong Xue,Yuansen Chen,Yikuan Liu,Xiangyan Meng,Haojun Fan
Extracorporeal membrane oxygenation (ECMO) is a life-saving technology for patients with severe cardiopulmonary failure. However, bleeding complications remain a major clinical challenge, largely due to high shear stress-induced Von Willebrand factor (VWF) dysfunction. Existing methods often lack timeliness and sensitivity for VWF detection in early bleeding risk assessment. Here, we proposed an electrochemical biosensor that combined Two-Step Synthesized graphene oxide-gold nanoparticles (rGO@AuNPs) nanocomposites modified screen-printed electrodes and a miniaturized wireless communication circuit for point-of-care testing (POCT) of VWF levels. The introduction of rGO@AuNPs significantly enhanced the sensing performance, achieving a low limit of detection of 0.39 pg/mL for VWF within 15 min. Moreover, there are Pearson correlation coefficients of 0.926 and 0.974 between the VWF biosensor and ELISA for porcine models and clinical samples. Notably, VWF degradation was associated with high shear stress, and VWF depletion was observed at the same time of APTT prolongation, suggesting its utility as a biomarker for bleeding risk. Its rapid, portable, and cost-effective design supports POCT, offering a promising tool for early monitoring and intervention in ECMO-related bleeding.
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PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2018
·
IF 3.235
Xiaoyu Zhao,Jianhua Ding,Wenlong Bai,Yanfei Wang,Yujia Yan,Yan Cheng,Juankun Zhang
A PEDOT:PSS/AuNPs/CA modified screen-printed carbon based disposable electrochemical sensor for sensitive and selective determination of carmine was studied. Enhanced electrode conductivity was achieved via a 3-step process: (i) construction of a conductive polymer (PEDOT: PSS) on a disposable screen-printed carbon electrode (SPCE) (ii) electropolymerization with gold nanoparticles (AuNPs) and (iii) modification with positively charged β-mercaptoethylamine (CA) in acidic solution using an Au S bond. The prepared nanomaterials were characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectrum analysis (EDX). The properties of the resulting sensor were examined by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). Experimental parameters associated with optimal detection conditions such as modes of electrode modification, ratio of PEDOT:PSS, pH of electrolyte, number of turns used for AuNPs polymerization, and CA concentrations were evaluated. The data revealed that the prepared sensor had a low detection limit (6.05 × 10 −9 mol/L) and could achieve linearity over a wide range of concentration (9.0 × 10 −9 to 3.9 × 10 −6 mol/L), thereby meeting the requirements for analysis of trace carmine. The reliability of our technique for practical applications was demonstrated by analyzing carmine in spiked beverages.
研究领域:
电分析化学
·
纳米材料科学
·
食品安全监测
·
传感器与生物传感器
·
环境分析化学
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Yingchun Li,Yaqin Zhang,Fange Kong,Chunxia Wang,Shanshan Chen,Jiasi Wang,Di Wang
Fusarium temperatum ( F. temperatum ) causes maize stalk rot disease, reducing grain yield and producing multiple harmful mycotoxins that threaten food safety and quality. However, current detection methods are limited by the requirement of well-trained personnel, complicated operations and expensive instruments. To address this challenge, gold nanoparticles (AuNPs)-based colorimetric assay using loop-mediated isothermal amplification (LAMP) coupled with CRISPR-Cas12a (Au-CRISPR) was tested for F. temperatum detection. The assay utilizes DNA-modified AuNPs as a colorimetric probe and the results can be readily observed by the naked eye. After optimization, the method can specifically detect as low as 100 copies/μL of target DNA or 10 −8 ng/μL of extracted DNA from F. temperatum . The detection platform allows high-throughput testing by integrating microtiter plates and microplate reader. Furthermore, a portable smartphone-based device was developed to realize point of care (POC) detection of F. temperatum in resource-limited settings. This simple, inexpensive and sensitive detection platform has great potential for the applications in field detection.
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高影响因子
危险品化学品经营许可证（带存储）