查找使用阿拉丁产品的科研文献

Preparation and characterization of BSA-loaded liraglutide and platelet fragment nanoparticle delivery system for the treatment of diabetic atherosclerosis

He Mingping,Fang Ming,Fan Limin,Maimaitijiang Alimujiang

Background Diabetic atherosclerosis is one of the main causes of morbidity and mortality worldwide, but its therapeutic options are limited. Liraglutide (LIR), a synthetic analog of GLP-1 approved as an anti-obesity drug by the FDA, has been reported as a promising drug for diabetic atherosclerosis. However, the main problem with LIR is its use that requires regular parenteral injections, which necessitates the improvement of drug delivery for increased efficiency and minimization of injection numbers. Results The objective of our present study was to prepare and characterize nanoparticles (BSA@LIR-PMF) for targeted drug delivery using LIR-encapsulated platelet membrane fragments (PMF) coated bovine serum albumin (BSA). We used various methods to characterize the prepared nanoparticles and evaluated their efficiency on diabetes-induced atherosclerosis in vitro and in vivo. The results showed that the nanoparticles were spherical and had good stability and uniform size with intact membrane protein structure. The loading and encapsulation rates (LR and ER) of BSA@LIR-PMF were respectively 7.96% and 85.56%, while the cumulative release rate was around 77.06% after 24 h. Besides, we also examined the impact of BSA@LIR-PMF on the proliferation, migration, phagocytosis, reactive oxygen species (ROS) levels, oxidative phosphorylation, glycolysis, lactate and ATP levels, and lipid deposition in the aortas. The results indicated that BSA@LIR-PMF could effectively inhibit ox-LDL-stimulated abnormal cell proliferation and migration, reduce the level of ROS and lactate concentration, and enhance the level of ATP, thereby improving oxidative phosphorylation in ox-LDL-treated cells. Conclusion BSA@LIR-PMF significantly inhibited diabetes-induced atherosclerosis. It was anticipated that the BSA@LIR-PMF nanoparticles might be used for treating diabetes-associated cardiovascular complications.

Horseradish peroxidase-catalyzed polyacrylamide gels: monitoring their polymerization with BSA-stabilized gold nanoclusters and their functional validation in electrophoresis

Chang Liao,Tao Li,Fengjiao Chen,Shaoying Yan,Liying Zhu,Hua Tang,Dan Wang

Polyacrylamide gel (PAG) is extensively used as a matrix for biomolecular analysis and fractionation. However, the traditional polymerization catalyst system N,N,N′,N′-tetramethylethylenediamine (TEMED)/ammonium persulphate (APS) of PAG presents non-negligible toxicity. Herein, we utilized the green and efficient bio-enzyme horseradish peroxidase (HRP) to catalyze the gel polymerization of polyacrylamide. At the same time, the efficacy of this gel system in separating nucleic acids and proteins was confirmed by applying the gel system in electrophoresis. This study aims to explore a higher biosafety polyacrylamide gel polymerization catalytic system which can be applied to electrophoresis technology. Furthermore, in order to differentiate between the bio-enzymatic catalytic system and the traditional toxic catalytic system during polymerization, aggregation-induced luminescence (AIE) of bovine serum albumin-stabilized gold nanoclusters (BSA-Au NCs) was used to monitor the polymerization reaction of the system. The results indicated that the fluorescence intensity of the polymeric system containing BSA-Au NCs increased with the polymerization of the monomers. Subsequently, we assessed whether certain components of nucleic acid electrophoresis and protein electrophoresis such as sodiumdodecylsulfate (SDS) and TBE buffer (Tris-boric acid, EDTA, pH 8.3) would affect the polymerization of the polyacrylamide gels catalyzed by the biological enzymes. The experimental conditions were also optimized to explore the optimal concentration of the ternary system of HRP, H2O2 and ACAC. Our results suggested that the bioenzyme-catalyzed system could be a feasible alternative to the TEMED/APS-catalyzed system, which also could provide new insights into the methods of monitoring the polymerization system.

Improved enzymatic assay for hydrogen peroxide and glucose by exploiting the enzyme-mimicking properties of BSA-coated platinum nanoparticles

He Shao-Bin,Chen Rui-Ting,Wu Yan-Yu,Wu Gang-Wei,Peng Hua-Ping,Liu Ai-Lin,Deng Hao-Hua,Xia Xing-Hua,Chen Wei

Platinum nanoparticles (Pt NPs) covered with a bovine serum albumin scaffold and a particle size of 1.5 nm (BSA-Pt S NPs) are shown to display enhanced multiple enzyme-mimicking activities including peroxidase, oxidase, and catalase-like activities. The peroxidase-like activity is characterized by robustness and low signal background. BSA-Pt S NPs were used to design colorimetric assays for H 2 O 2 and glucose. H 2 O 2 latter reacts with 3,3′,5,5′-tetramethylbenzidine in the presence of BSA-Pt S NPs to form a blue product with an absorption maximum at 652 nm. The assay works in the 5–250 μM H 2 O 2 concentration range. The glucose assay is based on its glucose oxidase-catalyzed oxidation to produce gluconic acid and H 2 O 2 which then is colorimetrically quantified. Response is linear in the 10–120 μM glucose concentration range, and the detection limit is 2 μM (at S/ N  = 3). The method correlates well with the glucose standard method (R 2  = 0.997 in the 95% confidence interval) which confirms that glucose in human serum has been successfully detected. Improved enzymatic assay for hydrogen peroxide and glucose by exploiting the enzyme-mimicking properties of BSA-coated platinum nanoparticles.

Investigation on the binding behavior between BSA and lenvatinib with the help of various spectroscopic and in silico methods

Bao-Li Wang,Song-Bo Kou,Zhen-Yi Lin,Jie-Hua Shi

Lenvatinib was a multi-tyrosine kinase inhibitor (TKI) used for treating advanced renal cell carcinoma and differentiated thyroid cancer. Multiple fluorescence spectroscopy including SSF, SF, 3DF, UV, FTIR, and molecular modeling were utilized for better understanding the binding interaction between lenvatinib and bovine serum albumin (BSA). SSF spectroscopy results showed lenvatinib quenched the intrinsic fluorescence of BSA in the form of the mixed mechanism. The binding constants (K b ) of lenvatinib on BSA were about 10 4  M −1 , indicating the affinity on BSA was moderate. Based on the analysis of the thermodynamic parameters, it can be inferred that lenvatinib spontaneously binds onto BSA and the hydrogen bonding and hydrophobic interactions are responsible for both bindings. The outcomes of UV, SF spectroscopy and FTIR showed the conformational change of BSA was caused due to binding lenvatinib. The outcomes of the molecular docking revealed that lenvatinib inserted into the hydrophobic groove between sub-domain IIA, IIB and IIIA, which further demonstrated the competition experiments result of ibuprofen and phenylbutazone markers. Furthermore, molecular docking and molecular dynamics (MD) simulations were performed to investigate the binding behavior of lenvatinib with BSA. The effect of some ions such as K + , Cu 2+ , Ca 2+ , Mg 2+ , Ni 2+ , Zn 2+ and Fe 3+ on the binding affinity was also discussed.

Uricase-Based Highly Sensitive and Selective Spectrophotometric Determination of Uric Acid Using BSA-Stabilized Au Nanoclusters as Artificial Enzyme

Huihui Zhao,Zhonghui Wang,Xue Jiao,Lichun Zhang,Yi Lv

Determination of the activity of telomerase in cancer cells by using BSA-protected gold nanoclusters as a fluorescent probe

Xu Yujuan,Zhang Peng,Wang Zhen,Lv Shaoping,Ding Caifeng

Gold nanoclusters (AuNCs) protected with a bovine serum albumin (BSA) coating are known to emit red fluorescence (peaking at 650 nm) on photoexcitation with ultraviolet light (365 nm). On addition of Cu(II) ions, fluorescence is quenched because Cu(II) complexes certain amino acid units in the BSA chain. Fluorescence is, however, restored if pyrophosphate (PPi) is added because it will chelate Cu(II) and remove it from the BSA coating on the AuNCs. Because PPi is involved in the function of telomerase, the BSA@AuNCs loaded with Cu(II) can act as a fluorescent probe for determination of the activity of telomerase. A fluorescent assay was worked out for telomerase that is highly sensitive and has a wide linear range (10 nU to 10 fM per mL). The fluorescent probe was applied to the determination of telomerase activity in cervix carcinoma cells via imaging. It is shown that tumor cells can be well distinguished from normal cells by monitoring the differences in intracellular telomerase activity. Gold nanoclusters (AuNCs) protected by bovine serum albumin (BSA) and displaying red photoluminescence were prepared as fluorescent probe for the determination of telomerase activity and used for imaging of cervix carcinoma (HeLa) cells.

Adsorption of IgG and BSA on Two Chromatographic Resins—Poly(ethylenimine)-4FF Resin and Tetrapeptide-poly(ethylenimine)-4FF Resin

Yu-Ming Fang,Dong-Qiang Lin,Shan-Jing Yao

Poly(ethylenimine) (PEI) as a long flexible chain has been well used in protein chromatography with the advantages of high spatial utilization and “chain delivery” effect. In this work, two resins, i.e., poly(ethylenimine)-4FF resin (PEI-4FF resin) and Ac-YFRH-PEI-4FF resin by grafting tetrapeptide to PEI-4FF resin, were prepared for the protein adsorption and chromatographic separation. The pH-dependent adsorption properties of PEI-4FF resin and Ac-YFRH-PEI-4FF resin were discovered by getting the data of adsorption capacities of bovine serum albumin (BSA) and immunoglobulin G (IgG) under different pH conditions and buffer systems. The results showed high binding capacities of BSA on PEI-4FF resins across a range of pH values (5.0–8.9) with almost very little adsorption of IgG over a pH range from 5.0 to 8.0, indicating that IgG could be separated by flow-through method using PEI-4FF resin. Since PEI has the advantage of having many flexible branched chains and Ac-YFRH (Ac-Try-Phe-Arg-His) as a peptide ligand has the advantages of comparatively high stability and low immunogenicity for antibody adsorption, Ac-YFRH-PEI-4FF resins were prepared by using PEI as the spacer arm and Ac-YFRH as the peptide ligand. The isotherm adsorption of the Ac-YFRH-PEI-4FF resin showed that the maximum binding capacity of IgG (qm = 123.6 mg/g resin, Kd = 0.10 mg/mL) was far more than that of BSA (qm = 62.0 mg/g resin, Kd = 0.25 mg/mL) at pH 8.0. Meanwhile, this tetrapeptide resin also showed high binding capacity of BSA (qm = 131.0 mg/g resin, Kd = 0.05 mg/mL) and low binding capacity of IgG (qm = 2.0 mg/g resin, Kd = 0.01 mg/mL) at pH 5.0, indicating that the separation of BSA and IgG could be achieved with the big differences in adsorption behaviors.

Biodegradable chitosan/alginate BSA-gel-capsules for pH-controlled loading and release of doxorubicin and treatment of pulmonary melanoma

Haijun Shen,Hui Shi,Meng Xie,Kun Ma,Bo Li,Song Shen,Xinshi Wang,Yi Jin

We engineered advanced biodegradable chitosan–alginate BSA-gel-capsules and demonstrated their applicability for the treatment of lung diseases. The pH-controlled loading and release of doxorubicin (DOX) was demonstrated. After treatment with DOX-loaded chitosan–alginate BSA-gel-capsules, effective cytotoxicity against B16F10 cells in vitro and higher efficacy of the pulmonary melanoma in vivo were revealed. In particular, the real-time biodistribution of DOX from DOX-loaded capsules was investigated for the first time, indicating the metabolism of capsules from the lung. These engineered capsules present new opportunities en route to the development of polyelectrolyte microcapsules for drug delivery.

Adsorption and catalytic performance of pipe growth rings from water distribution networks using 5-BSA as the target pollutant

Wanyi Huang,Yixing Yuan,Dan Zhong,Wencheng Ma,Yuan Yuan,Peng Zhang

In this study, we investigated the use of pipe growth rings from water distribution networks as a catalyst in heterogeneous Fenton-like oxidation processes. The major constituents of real pipe growth rings (α- and γ-FeOOH) were prepared and considered as a simulated growth ring (SGR). Its performance in removing 5-bromosalicylic acid (5-BSA), a novel phenolic halogenated disinfection byproduct , was examined. SGR exhibited strong catalytic ability and a certain degree of adsorption capacity. Under acidic conditions, the adsorption and oxidation efficiencies were 32.65% and 87.67%, respectively. Furthermore, 72.19% of 5-BSA could be oxidized even at pH 0 of 7. Kinetic characterizations at various temperatures revealed that both the adsorption and catalytic oxidation processes followed pseudo-second-order kinetic models and were surface-controlled with high activation energies (31.26 and 23.58 kJ mol −1 , respectively). Ecotoxicity analyses of the transformation products (TPs) showed that the SGR/H 2 O 2 system could effectively reduce the toxicity of 5-BSA. Moreover, the number of iron ions leaching from SGR was below 0.1 mg L −1 in all experiments. The results of this study support further investigation of using real pipe growth rings in off-line water treatment , as well water network contamination remediation.

Degradation Kinetic Studies of BSA@ZIF-8 Nanoparticles with Various Zinc Precursors, Metal-to-Ligand Ratios, and pH Conditions

Jia Gao,Wenhui Chu,Xuankai Ding,Lingzhi Ding,Qing Guo,Yongqian Fu

Nanosized zeolitic imidazolate framework particles (ZIF-8 nanoparticles [NPs]) have strong potential as effective carriers for both in vivo and in vitro protein drug delivery. Synthesis of ZIF-8 and stability of protein encapsulation within ZIF-8 are affected by several factors, notably the metal ion source and molar ratio. To systematically investigate these factors, we investigated such effects using BSA as a model test protein to synthesize BSA@ZIF-8 NPs at various metal-to-ligand (M:L) ratios. SEM, FTIR, XRD, and DLS were applied to characterize the morphology and structure of BSA@ZIF-8 NPs and their effects on protein loading capacity. Degradation kinetics and protein release behavior of BSA@ZIF-8 NPs were evaluated at pH 5.0 (to simulate the tumor environment) and pH 7.4 (to mimic the blood environment). Our objective was to define optimal combinations of the high protein loading rate and rapid release under varying pH conditions, and we found that (i) the yield of BSA@ZIF-8 NPs decreased as the M:L ratio increased, but the protein content increased. This highlights the need to strike a balance between cost-effectiveness and practicality when selecting ZIF-8 NPs with different molar ratios for protein-based drug formulation. (ii) BSA@ZIF-8 NPs exhibited cruciate flower-like shapes when synthesized using Zn(NO3)2 as the zinc precursor at M:L ratios of 1:16 or 1:20. In all other cases, the NPs displayed a regular rhombic dodecahedral structure. Notably, the release behavior of the NPs did not differ significantly between these morphologies. (iii) When Zn(OAc)2 was used as the zinc precursor, the synthesized ZIF-8 NPs exhibited a smaller size compared to the Zn(NO3)2-derived ZIF-8 NPs. (iv) The release rate and amount of BSA protein were higher at pH 5.0 compared to pH 7.4. (v) Among the different formulations, BSA@ZIF-8 with an M:L ratio of 1:16 at pH 5.0 was observed to have a shorter time to reach a plateau (0.5 h) and higher protein release, making it suitable for locally rapid administration in a tumor environment. BSA@ZIF-8 prepared from Zn(OAc)2 at an M:L ratio of 1:140 showed the slower release of BSA protein over a 24-h period, indicating its suitability for sustained release delivery. In conclusion, our findings provide a useful basis for the practical application of ZIF-8 NPs in protein-based drug delivery systems.

Enhancement of antifouling and separation properties of poly(m-phenylene isophthalamide) hybrid ultrafiltration membrane using highly crystalline poly(heptazine imide) nanosheets

Zhimeng Sun,Yiyong Yang,Zhongguo Zhang,Yue Shan,Zhifeng Hu,Jindi Yang,Xiaojing Ren

A highly crystalline phase g-C 3 N 4 , namely poly (heptazine imide) (PHI) was synthesized using molten salts. A high-performance ultrafiltration membrane was prepared by blending PHI nanosheets with Poly( m -phenylene isophthalamide) (PMIA) to obtain casting solution, and further prepared by phase conversion method. Hybrid PMIA membranes were prepared by incorporating 0.02–0.10 wt% of PHI, which were further characterized by SEM, AFM, XRD, FTIR, XPS, TGA and surface zeta potential. The hydrophilicity, water flux, and rejection of bovine serum albumin (BSA) of the membrane significantly increased. For instance, the water permeability of M4 (264.6 Lm −2 h −1 bar −1 ) was about 2.3 times that of bare PMIA membrane (113.6 Lm −2 h −1 bar −1 ), while maintaining a BSA rejection of 94.7 % for a 1000 mgL −1 BSA feed solution. Moreover, the M4 membrane showed excellent antifouling performance with a flux recovery rate of up to 88.7 % and lower irreversible fouling of 11.3 %. This study may provide reference for the modulation of highly crystalline g-C 3 N 4 structure and its application in membrane fabrication for water treatment.

Synthesis and characterization of FeIII/CoIII/CuII complexes with Schiff base ligand and their hybrid proteins, SOD activity and asymmetric catalytic oxidation of sulfides

Dan Mo,Jingnan Shi,Dandan Zhao,Yuzhen Zhang,Yifu Guan,Yunjun Shen,Hedong Bian,Fuping Huang,Shengfu Wu

Three transition metal complexes (Fe III , Co III , and Cu II ) were synthesized by hydrothermal method from bis(2-hydroxy-1-naphthalene-formaldehyde) 3,4-diaminobenzoic acid (H 3 L). They were characterized by infrared spectroscopy, elemental analysis, mass spectrometry. Artificial enzymes were constructed by hybridizing bovine serum albumin (BSA) with the metal complexes and characterized by MALDI-TOF-MS. The interaction of the metal complexes with BSA was studied by Raman spectroscopy, fluorescence spectroscopy, and circular dichroism spectroscopy. All the three metal complexes can cause the intrinsic fluorescence quenching of BSA, which is mainly static quenching. The addition of metal complexes changed the conformation of BSA and decreased the content of α–helix structure. The activity of SOD was measured by xanthine oxidase-NBT method. All metal complexes exhibited certain SOD activity, which increased after the formation of hybrid proteins. Asymmetric oxidation of thioether catalyzed by artificial enzymes was studied. The optimum reaction conditions were obtained by optimizing the reaction conditions. Substrate universality experiments were carried out under the optimum reaction conditions, showing good activity and enantioselectivity, with enantioselectivity up to 81%.