SENSORS AND ACTUATORS B-CHEMICAL
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2020
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IF 7.1
Congming Sun,Weiru Dong,Junxiang Peng,Xiaoliang Wan,Zhenli Sun,Dehao Li,Suhua Wang
Increasingly serious uranium pollution necessitates a new method for rapid qualitative and quantitative uranium detection. Some methods based on fluorescence spectroscopy and surface-enhanced Raman scattering (SERS) have been reported to detect uranyl ions (UO 2 2+ ), the main species of uranium. However, various factors affect the fluorescence selectivity and SERS quantitative analysis. Herein we report on a composite material for a dual-mode fluorescence–SERS detection protocol for simultaneous quantitative and qualitative analysis of UO 2 2+ , based on a layer-by-layer assembly strategy, combining the advantages of the two complementary techniques. The composite material consists of a Fe 3 O 4 @SiO 2 core coated by gold nanoparticles (FA) via electrostatic adsorption. The CdTe quantum dots (CdTe QDs) outside the FA are linked with amide bonds to form a satellite Fe 3 O 4 -Au@CdTe nanostructure (FA-CdTe). The fluorescence signal of FA-CdTe was selectively quenched by UO 2 2+ , with a detection limit as low as 1.2 nM; the material was also used as a SERS substrate to detect the Raman mode of uranyl at 828 cm −1 . Furthermore, this method has been applied for UO 2 2+ detection in seawater, river water, and tap water, demonstrating the broad application prospects and providing novel development concepts.
研究领域:
环境科学
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分析化学
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纳米技术
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材料科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MODERN PHYSICS B
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2022
·
IF 1.404
Yu-Hua Liu,Li-Ju Tan,Kun-Peng Wang,Jiang-Tao Wang
In this work, a sensitive fluorescent sensor for the detection of ciprofloxacin (CIP) was developed by wrapping the surface of CdTe quantum dots (QDs) with molecularly imprinted polymers (MIPs). The [email protected] were synthesized via a simple sol–gel method using 3-aminopropyltriethoxysilane (APTES) as the functional monomer and tetraethoxysilane (TEOS) as the crosslinker. The fluorescence emission of the probe was at 643 nm. Linear calibration curves were obtained in the range of 0.5–24 μ M for CIP with the detection limit of 0.23 μ M (S/N = 3). The fluorescent probe was successfully applied to the determination of CIP in seawater samples with the spiked recoveries ranging from 98.2% to 110.6% and the relative standard deviation (RSD) was less than 10.7%. It is the first time that QDs with molecularly imprinted polymers ( [email protected] ) were introduced for separation and detection of CIP in seawater and obtained good specific identification ability and potential practical value.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2024
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IF 8.5
Yimeng Zhang,Chuanliang Wang,Guo Wei,Xuesong Wang,Wanhui Liu,Gangqiang Yang,Peng Zhang,Qinglian Li,Xuhui Geng,Lingxin Chen,Zhihua Song
Malachite green (MG) possesses high toxicity, therefore, the detection of MG in fish tissues is of vital importance. A novel core–shell MIPs doped CdTe quantum dots coated silica nanoparticles (CdTe-MIP/SiO 2 NPs) were synthesized via a simple one-pot strategy. The materials were characterized carefully. The resulting CdTe-MIP/SiO 2 NPs were coated on the thin layer chromatography plate, and coupled with miniaturized fluorimeter for fluorescence detection of MG in fish samples. The resulting CdTe-MIP/SiO 2 NPs based system possessed good linearity (0.01 ∼ 20 μmol/L), high recoveries (98.36 %∼101.45 %) and low detection limit (3.7 nmol/L) for MG. Furthermore, CdTe-MIP/SiO 2 NPs based system were employed to measure fish samples spiked with MG, meanwhile, HPLC was utilized to evaluate the accuracy and reliability. And the paired t -test was conducted to evaluate differences between fluorescence method and HPLC, P > 0.05 means no significant difference was observed, the results demonstrated that both fluorescence method and HPLC are suitable for MG analysis.
研究领域:
分析化学
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环境科学
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纳米技术
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食品安全
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产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
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2022
·
IF 6.14
Jialing Zhou,Yumeng Zhang,Yaqian Du,Ruoxi Zhao,Shikai Liu,Wenting Li,Qingqing Wang,Piaoping Yang
The Fenton reaction has been widely utilized in tumor therapy and ferroptosis immunity in recent years. Therefore, the detection of hydrogen peroxide (H2O2), hydroxyl radicals (•OH), and iron(II) (Fe2+) in serum is urgently in need. A multifunctional combined carbon–CdTe quantum dots (C-CdTeQDs) probe is first established for the quick succession determination of H2O2 and Fe2+ in goat serum and human urine based on the fluorescence and colorimetric method. The double-emission fluorescence detection is performed by breaking the thioglycolic acid bond on the surface of CdTeQDs by H2O2 to quench the fluorescence at 594 nm. After the condition optimization, the fluorescence intensity is linearly related to H2O2 concentration, with a limit of detection of 58 nM. The colorimetric detection of •OH and Fe2+ concentrations is then continued by the Fenton reaction and the complexation reaction. Among them, the detection range of •OH is 0.01–0.1 μM with the limit of detection of 8 nM. Several advantages such as versatility, convenience, high selectivity, and sensitivity of the C-CdTeQDs probe indicated that it has great potential for the determination of H2O2 and Fe2+ in serum.
研究领域:
分析化学
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生物化学
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纳米技术
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医学诊断
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Hui Yingfei,Wang Mingyue,Liu Yinsheng,Peng Liping,Tian Jiaying,Ren Borong,Guo Hao,Yang Wu
In this study, a ratiometric fluorescent sensor CdTe QDs@ZIF-8 with butterfly spectra is successfully constructed by in situ encapsulating mercaptopropionic acid-modified CdTe quantum dots in zeolitic imidazolate framework-8 (ZIF-8) with a simple strategy, and used for the detection of tetracycline in fluorescence/smartphone colorimetry dual-mode. ZIF-8 not only reduces the agglomeration of the quantum dots but also surprisingly generates a new green fluorescence signal at 524 nm while the red fluorescence of the CdTe quantum dots at 650 nm quenches when tetracycline is added. The two opposing fluorescence signals create a butterfly-shaped fluorescence spectrum, allowing the sensor to detect tetracycline over a linear range of 0–70 μM with the detection limit (LOD) of 0.0155 μM by using a ratiometric fluorescence technique. What is more, based on the obvious color change of the fluorescent sensor gradually from red to green under UV light, a highly stable point-of-care testing sensor has been developed for on-site detection of tetracycline through color recognition by smartphones, which can be used for real-time detection of this antibiotic in the range of 0–1000 μM with the LOD of 0.0249 μM. This work provides a simple and efficient method for the on-site detection of tetracycline. Graphical Abstract
研究领域:
分析化学
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纳米技术
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 21
·5437-5444
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IF 3.286
Jiao Xiangyu,Zhou Yabin,Zhao Dan,Pang Duo,Wang Changtao,Du Hongwu,Wen Yongqiang,Zhang Xueji
To meet the need for high-throughput immunoassays, many multiplex fluorescent immunoassays have been proposed. Most of them need different kinds of fluorescent label indicators during the test. In this work, a novel indirect ELISA-inspired dual-channel fluorescent immunoassay based on 3-mercaptopropionic acid capped CdTe/ZnS quantum dots (QDs) was constructed. The ELISA wells were coated with two kinds of antigen–QD complex. When the primary antibodies were present in a sample, they mediated the binding of a secondary antibody–DNA–gold nanoparticle complex to the antigen–QD complex. Then the gold nanoparticles quenched the fluorescence of the QDs and a decrease in fluorescence intensity was observed. Thus, the amount of primary antibody could be estimated from the decrease of fluorescence intensity. Owing to the wide absorption range and the relatively narrow emission band of the QDs, the dual-channel fluorescent immunoassay system could work at the same excitation wavelength and the emission wavelengths of each channel had no interference. As a result, two different kinds of primary antibody could be detected at the same time in one ELISA well, which simplified the operation and greatly improved the efficiency. Besides, only one type of secondary antibody needs to be added to the prepared microtiter plates, which further simplified the operation during the detection procedure. This dual-channel fluorescent immunoassay system will provide new insights into high-throughput immunodetection.
Graphical abstract
研究领域:
生物化学
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分析化学
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纳米技术
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免疫学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2022
·Vol. 38
·期 10
·1305-1312
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IF 1.967
Liu Piaotong,Hao Rusi,Sun Wenliang,Lin Ziyi,Jing Tianfeng,Yang Haizhen
A facile and efficient “bottle-around-ship” approach for preparing the ratiometric fluorescent probe has been developed by encapsulating the red-colored fluorescence CdTe quantum dots (QDs) and blue-colored fluorescence graphitic carbon nitride quantum dots (g-CNQDs) into the zeolitic imidazolate metal–organic frameworks (ZIF-8) in one step. At a single excitation of 360 nm, the obtained probe ZIF-8@g-CNQD/CdTe shows the dual-emission peaked at 450 and 633 nm, respectively. The red emission of CdTe QDs is selectively quenched by the Hg 2+ , whereas the blue fluorescence of g-CNQDs as an internal reference is insensitive, resulting in an apparent color transformation from pink to blue for special recognition of Hg 2+ . By this approach, the relative fluorescence intensity ratio ( F 633 / F 450 ) decreased linearly with increasing Hg 2+ concentration in the 0.2–3.5 μM range with a low limit of detection (LOD) of ~ 46 nM. Therefore, we demonstrate that this “bottle-around-ship” process provides a new strategy for the construction of ratiometric fluorescent Hg 2+ probes with good simplicity, high efficiency, and excellent stabilities. Moreover, the obtained Hg 2+ fluorescent probe shows good results in the detection of actual samples. Graphical abstract
研究领域:
材料科学
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分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Bin Chen,Junjie Liu,Tong Yang,Lin Chen,Jia Hou,Changhao Feng,Cheng Zhi Huang
Ag + as one of the most commonly seen toxic heavy metal ions is involved in numerous vital biological processes, which would cause fatal damages and environmental contamination when Ag + is excessive. In the present work, CdTe quantum dots (QDs) with green, orange, and red emission capped by mercaptoacetic acid (TGA) were synthesized at one time by controlling the synthesis time and utilized for Ag + detection. Both fluorescence spectral red-shift and intensity decrease could be used for Ag + discrimination. Fluorescence lifetime , Zeta potential , and XRD, etc. were carried out to analyze the detection mechanism. Results displayed that surface passivation and electron transfer due to binding effects of Ag + to Te atom on traps of QDs could be relied on to explain the sensing mechanism. Additionally, in accordance with PCA analysis, Ag + could be also be successfully differentiated from Hg 2+ and the other metal ions. Importantly, a home-made portable device based on a 32 bit embed Micro Control Unit (MCU) system was first proposed for Ag + detection. The power supply system adopt the mini-sized lithium cell instead of the power supply system, which ensure its practical applicability. The relative position of light source and detector is set at 90° to minimize the interference. According to the detection results, the linear detection range using the device was from 5 nM to 200 nM (with a larger coefficient of determination, R 2 ), and the detection limit was calculated to be about 5 nM, which indicated that this proposed method and device could fulfil the practical application requirements.
研究领域:
环境科学
·
分析化学
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材料科学
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电气工程
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 10
·1-9
·
IF 6.232
Huang Lingting,Wang Jun,Wang Qingshui,Tang Dianping,Lin Yao
A paper-based visual fluorescence immunoassay is presented for the detection of matrix metalloproteinase-7 (MMP7) that is related to renal cancer. The method is based on the distance-dependent fluorescence quenching of CdTe quantum dots (QDs) on a nitrocellulose membrane by Ag + following a sandwich-type immunoreaction on microtiter wells using silver nanoparticle (AgNP)–labeled secondary antibody– and primary antibody–coated microtiter wells. The silver nanoparticles captured in the well are dissolved with HNO 3 , while the quenching effect of QDs is based on silver ion-exchange reaction under 365-nm excitation light irradiation. Increasing concentration of released Ag + , thus higher concentration of the protein, leads to an increased distance of quenching on the nitrocellulose membrane. The paper-based immunoassay by combination of AgNP-assisted ion-exchange reaction with QD gives good distance-dependent responses and allows the detection of MMP7 at a concentration as low as 7.3 pg mL −1 . The coefficients of variation are less than 6.9% and 12.4% for intra-assay and inter-assay, respectively. High specificity and long-term stability are achieved during the assay. Importantly, the testing of human serum samples using our strategy shows well-matched results with commercial human MMP7 ELISA kits.
A distance-dependent visual immunoassay is developed for the determination of serum matrix metalloproteinase-7 on CdTe quantum dot–impregnated paper with silver ion-exchange reaction.
研究领域:
分析化学
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生物化学
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医学诊断
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Yizhong Shen,Shaopu Liu,Ling Kong,Dan Li,Youqiu He
Thioglycolic acid-capped (TGA-capped) CdTe/CdS quantum dots (QDs) were prepared in the water phase. The interaction of TGA-CdTe/CdS QDs with polymyxin B sulfate (POBS) was investigated by ultraviolet–visible (UV–vis) absorption, fluorescence (FL), resonance Rayleigh scattering (RRS) spectroscopy and the measurement of fluorescence lifetime . Under optimum conditions, not only the UV–vis absorption and fluorescence spectra were changed, but also the RRS intensity was greatly enhanced. These phenomena offered useful techniques for the determination of POBS with fluorescence quenching and RRS methods . The linear ranges and detection limits of POBS were 0.09–5.25 μg mL −1 and 27.5 ng mL −1 for the fluorescence quenching technique, 0.02–6.0 μg mL −1 and 6.36 ng mL −1 for the RRS technique. Among them, the RRS approach obtained the higher sensitivity. Accordingly, a novel rapid, convenient, and highly sensitive RRS method for the determination of POBS was proposed and applied to detect POBS in pharmaceutical preparations with satisfactory results. Furthermore, the suitable reaction conditions, affecting factors as well as the influence of coexisting substances were studied. Some possible reaction mechanisms were also discussed.
研究领域:
分析化学
·
生物医学工程
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Wenqi Zhang,Jiang Ling,Di Wen,Zijia Cheng,Suiping Wang,Yanjun Ding
The early diagnosis of acute myocardial infarction (AMI) was a tough nut in both clinical medicine and forensic science since the occurrence of gross changes needed at least 12 h. Compared to other diagnostic methods, microRNA was reported as an earlier cardiac biomarker with higher sensitive. In this work, we designed a novel fluorescent analytical strategy using a dual-emission probe based on T7 Exo-assisted target recycling amplification for simultaneous detection of AMI-related miR-199a and miR-499, which could indicate myocardial ischemia or damage in AMI. The dual-emission probe was conducted by yellow-emission and red-emission CdTe quantum dots capped with beta-cyclodextrin. The florescence of probe was firstly quenched by graphene oxide (GO) and then recovered in the presence of target miR-199a and miR-499. Under the optimized conditions, the limit of detection as low as 0.1 pM was obtained and the overall test could be performed within 50 min. Besides, the simultaneous detections of microRNAs in spiked serum samples were achieved with the recovery of 81.3–109.6%, which suggested the potential method for diagnosis in practical. The advantages of this method include easy operation, high accuracy and low labor cost, which makes point-of-care test possible both in hospital and forensic application.
研究领域:
临床医学
·
法医学
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生物技术
·
纳米技术
·
分子生物学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Jing Wang,Daquan Li,Yu Qiu,Xinyue Liu,Xin Zhang,Liang Huang,Huimin Wen,Jun Hu
Developing a reliable fluorescent nanosensor for cyanide anions (CN − ), with the potential to enable visual discrimination by naked eye, is highly sought after yet is limited by low sensitivity and selectivity. Herein based on the synergistic effect of fluorescence recovery and enhancement, a specific dual-emission nano-system was designed for ultrasensitive detection of CN − , by employing carbon dots (peaking at 443 nm) as a reference and N-acetyl-L-cysteine (NALC)-capped CdTe quantum dots (QDs, peaking at 611 nm) as a reporter. The red fluorescence of CdTe QDs is first quenched by Cu 2+ primarily with electron transfer. Upon addition of CN − , CN − coordinates with Cu 2+ to form [Cu(CN) n ] (n−1)− complex, causing the Cu 2+ to detach from the QDs, and consequently fluorescence recovery. More importantly, the CN − can undergo a nucleophilic addition reaction with the carbonyl group of NALC ligands, inducing 1.3-fold fluorescence enhancement towards the original QDs while the blue fluorescence of carbon dots remains constant. This results the fluorescence intensity ratios ( I 611 / I 443 ) are proportional to the concentrations of CN − in the ranges of 0.02―10 and 15―80 μM, and an ultra-low detection limit down to 10.35 nM is achieved. By using both Cu 2+ -promoted complexation and addition reaction as recognition units, the present method also showed excellent selectivity for CN − over other coexisting anions. Especially, we have already demonstrated, by spiked tests, the practicability of monitoring the concentration changes of CN − in both environmental water and cassava samples, and further realized visual monitoring of CN − changes in aqueous solution and test paper.
研究领域:
分析化学
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纳米技术
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环境科学
·
材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）