JOURNAL OF CONTROLLED RELEASE
·
2016
·
IF 7.441
Wenjun Zhang,Feihu Wang,Yun Wang,Jining Wang,Yanna Yu,Shengrong Guo,Rongjun Chen,Dejian Zhou
A thiolated pH-responsive DNA conjugated gold nanorod (GNR) was developed as a multifunctional nanocarrier for targeted, pH-and near infrared (NIR) radiation dual-stimuli triggered drug delivery. It was further passivated by a thiolated poly(ethylene glycol)-biotin to improve its cancer targeting ability by specific binding to cancer cell over-expressed biotin receptors. Doxorubicin (DOX), a widely used clinical anticancer drug , was conveniently loaded into nanocarrier by intercalating inside the double-stranded pH-responsive DNAs on the GNR surface to complete the construction of the multifunctional nanomedicine . The nanomedicine can rapidly and effectively release its DOX payload triggered by an acidic pH environment (pH ~ 5) and/or applying an 808 nm NIR laser radiation. Compared to free DOX, the biotin-modified nanomedicine displayed greatly increased cell uptake and significantly reduced drug efflux by model multidrug resistant (MDR) breast cancer cell lines (MCF-7/ADR). The application of NIR radiation further increased the DOX release and facilitated its nuclear accumulation. As a result, this new DNA-GNR based multifunctional nanomedicine exerted greatly increased potency (~ 67 fold) against the MDR cancer cells over free DOX.
研究领域:
纳米技术
·
癌症治疗
·
药物递送系统
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·10646-10646
·
IF 3.8
Qinghua Xie,Xuemei Wang,Gaorui Zhang,Dawei Zhou,Yuxuan Zhao,Hong Liu,Jiazhi Duan,Dexin Yu,Yuanhua Sang
Individual theranostic agents with dual-mode MRI responses and therapeutic efficacy have attracted extensive interest due to the real-time monitor and high effective treatment, which endow the providential treatment and avoid the repeated medication with side effects. However, it is difficult to achieve the integrated strategy of MRI and therapeutic drug due to complicated synthesis route, low efficiency and potential biosafety issues. In this study, novel self-assembled ultrasmall Fe 3 O 4 nanoclusters were developed for tumor-targeted dual-mode T 1 /T 2 -weighted magnetic resonance imaging (MRI) guided synergetic chemodynamic therapy (CDT) and chemotherapy. The self-assembled ultrasmall Fe 3 O 4 nanoclusters synthesized by facilely modifying ultrasmall Fe 3 O 4 nanoparticles with 2,3-dimercaptosuccinic acid (DMSA) molecule possess long-term stability and mass production ability. The proposed ultrasmall Fe 3 O 4 nanoclusters shows excellent dual-mode T 1 and T 2 MRI capacities as well as favorable CDT ability due to the appropriate size effect and the abundant Fe ion on the surface of ultrasmall Fe 3 O 4 nanoclusters. After conjugation with the tumor targeting ligand Arg-Gly-Asp (RGD) and chemotherapy drug doxorubicin (Dox), the functionalized Fe 3 O 4 nanoclusters achieve enhanced tumor accumulation and retention effects and synergetic CDT and chemotherapy function, which serve as a powerful integrated theranostic platform for cancer treatment.
研究领域:
纳米技术
·
生物医学工程
·
分子影像
·
癌症治疗
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2018
·
IF 8.806
Zhenqi Jiang,Yuchen Tian,Dingying Shan,Yinjie Wang,Ethan Gerhard,Jianbi Xia,Rong Huang,Yan He,Aiguo Li,Jianchao Tang,Huimin Ruan,Yong Li,Juan Li,Jian Yang,Aiguo Wu
Nanoparticle-based tumor therapies are extensively studied; however, few are capable of improving patient survival time due to premature drug leakage, off target effects, and poor tissue penetration. Previously, we successfully synthesized a novel family of Y 1 receptor (Y 1 R) ligand modified, photoluminescent BPLP nanobubbles and nanoparticles for targeted breast cancer ultrasound imaging ; however, increased accumulation could also be observed in the liver, kidney, and spleen, suggesting significant interaction of the particles with macrophages in vivo . Herein, for the first time, we imparted antiphagocytosis capability to Y 1 R ligand functionalized BPLP-WPU polymeric micelles through the incorporation of a CD47 human glycoprotein based self-peptide. Application of self-peptide modified, DOX loaded micelles in vivo resulted in a 100% survival rate and complete tumor necrosis over 100 days of treatment. In vivo imaging of SPION loaded, self-peptide modified micelles revealed effective targeting to the tumor site while analysis of iron content demonstrated reduced particle accumulation in the liver and kidney, demonstrating reduced macrophage interaction, as well as a 2-fold increase of particles in the tumor. As these results demonstrate, Y 1 R ligand, self-peptide modified BPLP-WPU micelles are capable of target specific cancer treatment and imaging, making them ideal candidates to improve survival rate and tumor reduction clinically.
研究领域:
癌症治疗
·
纳米医学
·
药理学
·
分子影像
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Extreme Manufacturing
·
2024
·
IF 16.1
Youdi Hu,Yanlei Hu,Zhenyu Wang,Jiale Yong,Wei Xiong,Dong Wu,Shixiang Xu
Surface enhanced Raman scattering (SERS) enabled trace molecules detection has important application prospects. By structuring/modifying the surface of SERS substrate, molecules in highly-diluted solution can be concentrated into localized active area for highly sensitive detection. However, subject to the difficulty of fabrication process, it remains challenging to balance hot-spots construction and concentration capacity to molecules simultaneously. Therefore, preparing SERS substrate with dense ordered hot-spots and efficient concentration capacity is of great significance for highly sensitive detection. Herein, we propose the Ag and fluoroalkyl modified hierarchical armour substrate (Ag/F-HA), which has a double-layer stacking design to combine analyte concentration with hotspot construction. The micro armour structure fabricated by femtosecond-laser processing to serve as a superhydrophobic and low-adhesive surface to concentrate molecules, while anodic aluminum oxide (AAO) template creates nanopillars array serving as dense and ordered hot spots. Under the synergy action of hot-spots and molecule concentration, Ag/F-HA achieves the detection limit down to 10−7 M of Doxorubicin (DOX) molecules with a relative standard deviation (RSD) of 7.69%. Additionally, Ag/F-HA exhibits the excellent robustness to resist external disturbance such as liquid splash or abrasion. Based on our strategy, the SERS substrates with directional analyte concentration are further explored by patterning microcone array with a defect. This work opens a way to the realistic implementation of SERS in diverse scenarios.
研究领域:
分析化学
·
材料科学
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED MATERIALS
·
2024
·
IF 27.4
Kangkang Liu,Lining Wang,Jing Peng,Yuanji Lyu,Yiming Li,Dengyi Duan,Wenyi Zhang,Guojiang Wei,Taipeng Li,Yuanjie Niu,Yang Zhao
Intravesical Bacillus Calmette–Guérin (BCG) is a well-established strategy for managing high-risk nonmuscle-invasive bladder cancer (NMIBC); however, over half of patients still experience disease recurrence or progression. Although the combined intravesical instillation of various chemotherapeutic drugs is implemented in clinical trials to enhance the BCG therapy, the outcome is far from satisfying due to severe irritative effects and treatment intolerance at high doses. Therefore, it is adopted the “biotin–streptavidin strategy” to doxorubicin (DOX)-encapsulated nanoparticles within live BCG bacteria (DOX@BCG) to improve treatment outcomes. Adherence of BCG to the bladder epithelium helps precisely target DOX@BCG to the local tumor cells and simultaneously increases intratumoral transport of therapeutic drugs. DOX@BCG effectively inhibits cancer progression and prolongs the survival of rats/mice with orthotopic bladder cancer owing to synergism between BCG-immunotherapy, DOX-chemotherapy, and DOX-induced immunogenic tumor cell death; furthermore, it exhibits improved tolerance and biosafety, and establishes antitumor immunity in the tumor microenvironment. Therefore, the drug-loaded live BCG bacterial delivery system holds considerable potential for clinical translation in the intravesical treatment of bladder cancer.
研究领域:
肿瘤学
·
药理学
·
免疫治疗
·
纳米技术
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2012
·Vol. 5
·期 1
·253-261
·
IF 5.914
Xiaojiao Kang,Dongmei Yang,Yunlu Dai,Mengmeng Shang,Ziyong Cheng,Xiao Zhang,Hongzhou Lian,Ping'an Ma,Jun Lin
In this study, multifunctional poly(acrylic acid) modified lanthanide-doped GdVO4 nanocomposites [PAA@GdVO4: Ln3+ (Ln = Yb/Er, Yb/Ho, Yb/Tm)] were constructed by filling PAA hydrogel into GdVO4 hollow spheres via photoinduced polymerization. The up-conversion (UC) emission colors (green, red and blue) can be tuned by changing the codopant compositions in the matrices. The composites have potential applications as bio-probes for cell imaging. Meanwhile, the hybrid spheres can act as T1 contrast agents for magnetic resonance imaging (MRI) owing to the existence of Gd3+ ions on the surface of composites. Due to the nature of PAA, DOX-loaded PAA@GdVO4:Yb3+/Er3+ system exhibits pH-dependent drug releasing kinetics. A lower pH offers a faster drug release rate. Such character makes the loaded DOX easily released at cancer cells. The cell uptake process of drug-loaded composites was observed by using confocal laser scanning microscopy (CLSM). The results indicate the potential application of the multifunctional composites as theragnostics (effective bimodal imaging probes and pH-responsive drug carriers).
研究领域:
材料科学
·
纳米技术
·
生物医学工程
·
分子生物学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2018
·
IF 3.862
Weixia Xu,Xiaofeng Gao,Pengjin Ge,Fuquan Jiang,Xiaokun Zhang,Jingjing Xie
Multidrug resistance (MDR), as a major obstacle in cancer therapy, has resulted in over 90% of cancer chemotherapeutic failure. Mesoporous silica nanospheres (MSNs) have been demonstrated to be tuned with large pore sizes, mediating the MDR-reversal effects. However, the study that surface functionality of the large pore sized-MSNs affects the MDR-overcoming effects hasn’t been extensively studied. In this study, we developed a new dendrimer-like MSNs delivery system based on a rational synthesis strategy and further modified MSNs with various surface functionalities to evaluate their roles in overcoming cancer MDR. Our results showed that the small particle sized-MSNs could be fabricated with dendrimer-like internal structure, resulting in the large pore size of 9 nm. Surface functionality of MSNs, especially hydroxylation and carboxylation, largely improved the intra-nuclear delivery and therapeutic efficiency of DOX for MCF7/ADR cells, which was not up to inhibiting P-gp expression but significantly increasing the intracellular drug accumulation of over 90% even under the strong drug efflux. This study indicates that surface functionality design strategy may display the potential of the large pore sized-MSNs as the efficient chemotherapeutic carriers to combat MDR.
研究领域:
纳米技术
·
药学
·
癌症治疗
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Wei Fang,Zhuyuan Wang,Shenfei Zong,Hui Chen,Dan Zhu,Yuan Zhong,Yiping Cui
A type of pH-controllable drug carrier is demonstrated based on mesoporous silica nanoparticles and chitosan/poly (methacrylic acid), which can simultaneously serve as the surface enhanced Raman scattering (SERS) traceable drug carriers for targeting cancer cells. The pH-sensitive releasing characteristics can be achieved by coating the nanoparticles with a layer of chitosan/poly (methacrylic acid) (CS-PMAA), while strong SERS signals can be obtained from the SERS reporter tagged Ag nanoparticles in the core. Our experimental results show that doxorubicin (DOX) was effectively encapsulated into the nanocarriers and can be released in response to the ambient pH value. Specifically, an increased amount of DOX release was observed at lower pH value. In addition, the composite nanoparticles were conjugated with transferrin (Tf) to target transferrin receptor (TfR)-overexpressed cancer cells. The targeting ability as well as the intracellular location of the drug carrier was investigated through SERS mapping while the distribution of DOX was monitored by fluorescence images. The results show that the demonstrated drug carrier can simultaneously fulfill the functionalities of pH-responsive drug release, SERS-traceable characteristics and cancer cells targeting, which has a unique potential for the pH-controllable drug delivery nanosystems .
研究领域:
纳米技术
·
药学
·
癌症研究
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xu Zhang,Yanbao Zhao,Liuqin Cao,Lei Sun
In this work, pH/redox-responsive silica-based nanocarriers were constructed for drug delivery. Lemon-like porous silica (L-pSiO 2 ) sample presents uniform sphere with size of 160–180 nm and processes thiol-functionalized center-radial inner pores and amine-functionalized shell. Cystine (Cys) molecules were conjugated on the surface of L-pSiO 2 to seal the drug-loaded pores. To minimize the premature release, ZnO quantum dots (QDs) were further used to seal the nanopores of pSiO 2 NSs , which could act as gates and fluorescence probes. Both in vitro cellular cytotoxicity and hemolysis assay demonstrated that the L-pSiO 2 /Cys nanospheres (NSs) were highly biocompatible. The L-pSiO 2 /Cys NSs have high specific surface area (201 m 2 g −1 ) and large pore volume (0.426 cm 3 g −1 ) and are suitable to utilize as drug carriers. The DOX-loaded L-pSiO 2 /Cys NSs displayed more efficient cytotoxic to HepG2 cells than free DOX . Both L-pSiO 2 /Cys and L-pSiO 2 /Cys/ZnO carriers displayed low premature and pH/redox-responsive release. The DOX release from L-pSiO 2 /Cys followed the first-order kinetics model with high correlation coefficient. Interestingly, the L-pSiO 2 /Cys carrier displayed GSH-responsive degrading the inner core and pH-responsive dissolving silica shell at physiological environment.
研究领域:
纳米技术
·
药学
·
材料科学
·
生物材料
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
·
2020
·
IF 10.317
Jing Yan,Qinghua Wu,Ziyin Zhao,Jianhua Wu,Huan Ye,Qiujun Liang,Zhuchao Zhou,Mengying Hou,Xudong Li,Yong Liu,Lichen Yin
The anticancer performance of nanomedicine is largely impeded by insufficient intratumoral penetration. Herein, tumor microenvironment (TME)-amendatory and self-adaptive nanoclusters (NCs) capable of cancer-associated fibroblasts (CAFs) depletion and size/charge conversion were engineered to mediate light-assisted, hierarchical intratumoral penetration. Particularly, large-sized NCs (~50 nm) were prepared via self-assembly of FAP-α-targeting peptide-modified, 1 O 2 -sensitive polymers, which were further used to envelope small-sized dendrimer (~5 nm) conjugated with Ce6 and loaded with DOX (DC/D). After systemic administration, the NCs efficiently targeted CAFs and generated lethal levels of 1 O 2 upon light irradiation, which depleted CAFs and concomitantly dissociated the NCs to liberate small-sized, positively charged DC/D. Such stroma attenuation and NCs transformation collectively facilitated the delivery of DC/D into deeper regions of CAF-rich tumors, where DOX and 1 O 2 provoked synergistic anti-cancer efficacies. This study provides an effective approach to facilitate the tumor penetration of nanomedicine by concurrently and spatiotemporally reconfiguring the nano-properties and remodeling the TME.
研究领域:
纳米技术
·
癌症治疗
·
药学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
Asian Journal of Pharmaceutical Sciences
·
2025
·
IF 11.9
Zhiyuan Tang,Yuening Sun,Quanhua Yi,Qian Ding,Yang Ding,Jianfei Huang
The effective intracellular accumulation of doxorubicin (DOX) is crucial for improving antitumor efficacy, which is severely impeded by limited drug penetration, uncontrollable drug release, and drug resistance. In this study, a thermal-deformative polymer embedding ultrasmall ceria (CeO 2 ) was rationally designed for deep tumor drug shuttling and hypoxia reversal to improve chemotherapy. Structurally, the CeO 2 nanozyme was covalently grafted with a polymer of p(NIPAM-co-AM) that could sharply shrink for DOX loading, which was consolidated with polydopamine (PDA) film encapsulation. Thereafter, a tumor penetration guide of apolipoprotein A-I (apoA-I) conjugated iRGD peptide (apoA-I-iRGD) was further decorated onto the PDA shell via Michael addition for preparing CeO 2 P/DOX@iAPDA. With the aid of apoA-I-iRGD, CeO 2 P/DOX@iAPDA penetrated both the tumor spheroids (∼78 μm) and the tumors of the mouse model deeply. After internalization by tumor cells and triggering by low pH in lysosomes, rapid DOX release was achieved by peeling off the PDA shell and thermosensitive deformation of p(NIPAM-co-AM). CeO 2 P/DOX@iAPDA provided 66.4% tumor suppression in 4T1-derived tumor spheroids and 63.2% in 4T1-tumor-bearing mice, respectively. Preliminary mechanistic research involving western blotting and immunohistochemistry revealed that CeO 2 P/DOX@iAPDA reversed resistance through the through HIF-1α-P-gp/lipid axis. Collectively, this study intelligently integrated CeO 2 nanozymes, temperature-sensitive polymers, and imitated biochemical modifications to improve chemotherapy for breast cancer.
研究领域:
纳米技术
·
癌症治疗
·
药学科学
·
生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Zhihao Wang,Qiuli Cheng,Xingda Jiang,Xinyang Chu,Bingcan Li,Leitao Zhang,Wenlan Wu,Junbo Li,Ravin Narain
Nanoparticles-based drug delivery systems show great promise for cancer therapy due to the advantages of crossing physiological barrier, high drug availability and good stability. However, deep tumor penetration, long blood circulation, and rapid drug release remain intractable problems for nanocarriers. In this work, we report a tumor microenvironment-sensitive nanodrug delivery system containing both acidic pH and hypoxia response sites to achieve enhanced drug permeability and on-demand release for precise cancer therapy. The covalent organic frameworks (COFs) with introduced azobenzene (azo) groups were used as employed as nanocarrier, and modified by pH-sensitive sulfamide-based zwitterionic polymers (PBA), followed by loading with the chemotherapeutic drug doxorubicin (DOX) to obtain COF-PBA@DOX. Copolymer PBA achieved charge conversions in tumor acidic environment, resulting in a positive charge on the nanocarrier surfaces, thus facilitating the augmented tumor accumulation. Meanwhile, azo which was formed the COF carrier, could be reduced to amines by the highly expressed azoreductase under tumor hypoxia conditions, resulting in nanoparticle dissociation and the drug-controlled release at the solid tumor sites. The fabricated dual-responsive nanomedicine, based on the surface charge-reversal of zwitterion in the acidic environment of tumor, can realize the accumulation and targeted drug release at the tumor site, which has great potential as a nanocarrier in tumor therapy.
研究领域:
纳米技术
·
癌症研究
·
药物递送系统
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）