JOURNAL OF HAZARDOUS MATERIALS
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2021
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IF 10.588
Yuhao Wu,Junke Wang,Tianxin Zhao,Jiadong Chen,Lian Kang,Yuexin Wei,Lindong Han,Lianju Shen,Chunlan Long,Shengde Wu,Guanghui Wei
Di-(2-ethylhexyl) phthalate (DEHP) is an extensively used plasticizer and has been shown to cause reproductive dysfunction in humans and model animals. However, the exact mechanisms of testicular injury induced by DEHP exposure have not been fully clarified. Using gas chromatography-mass spectrometry, we found that mono-2-ethylhexyl ester (MEHP, a major biometabolite of DEHP) and DEHP concentrations were elevated in mouse serum after DEHP exposure. Using RNA-seq, we found that ferroptosis and HIF-1 signaling pathways might be involved in testicular injury due to prepubertal DEHP exposure. Subsequent Western blotting, ferrous iron and MDA measurements, and immunofluorescence of testicular sections verified the RNA-seq findings. Consistently, based on the RNA-seq findings, we found that ferroptosis and HIF-1 signaling pathways might play crucial roles in Leydig and Sertoli cell injury due to MEHP exposure in vitro. Further experiments also confirmed ferroptosis in Leydig and Sertoli cells. Using Western blotting, cellular immunofluorescence and ChIP-qPCR, we found that MEHP exposure caused HIF-1α accumulation and stabilization, resulted in HIF-1α translocation into the nucleus, and induced HIF-1α/ Hmox1 binding in Leydig and Sertoli cells. To clarify whether HIF-1α plays a pivotal role in MEHP-induced ferroptosis, we knocked out Hif-1α using the CRISPR/Cas9 technique. We found that Hif-1α knockout rescued MEHP-induced ferroptosis. In summary, our findings certified that prepubertal DEHP exposure led to ferroptosis in mouse testes via the HIF-1α/HO-1 signaling pathway.
研究领域:
毒理学
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生殖生物学
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分子生物学
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DOI
PubMed
高影响因子
LIFE SCIENCES
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2018
·
IF 3.234
Runxiao Lv,Lili Du,Lixin Zhang,Zhiqiang Zhang
Aims Spinal cord injury (SCI) is one of the most devastating central lesions, resulting in serious locomotor deficit. Polydatin is a glucoside of resveratrol with proven anti-cardiovascular, anti-inflammatory and anti-oxidative properties. The main purpose of this study was to investigate whether polydatin could alleviate SCI in rats and explore the underlying mechanisms. Materials and methods SCI rats induced by a weight-drop device were treated with intraperitoneal injection of 20 or 40 mg/kg polydatin. Then the locomotor function of SCI rats was evaluated by the Basso, Beattie and Bresnahan locomotor rating scale, spinal cord edema was measured by the wet/dry weight method, oxidative stress markers were detected by commercial kits and cell apoptosis status was measured by TUNEL staining. In addition, reactive oxygen species (ROS) generation, lactate dehydrogenase (LDH) production and apoptosis status were detected in murine microglia BV2 cells treated with 100 ng/ml lipopolysaccharides (LPS) and 4.0 μM polydatin. The expression of apoptosis-related proteins involved in nuclear factor E2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway was measured by western blot. Key findings Our data showed that polydatin treatment improved locomotor performance of SCI rats, as well as reduced oxidative stress and inhibited apoptosis by enhancing Nrf2/HO-1 signaling. In addition, polydatin was found to up-regulate Nrf2 activity and the inhibitory effects of polydatin on oxidative stress and apoptosis in LPS-stimulated BV2 microglia was neutralized by silencing Nrf2 using specific siRNA. Significance We demonstrate that polydatin may protect the spinal cord from SCI by suppression of oxidative stress and apoptosis via improving Nrf2/HO-1 signaling in microglia.
研究领域:
神经科学
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药理学
·
分子生物学
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DOI
PubMed
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
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2019
·
IF 3.407
Li-Ming Yu,Xue Dong,Xiao-Dong Xue,Jian Zhang,Zhi Li,Hong-Jiang Wu,Zhong-Lu Yang,Yang Yang,Hui-Shan Wang
Punicalagin has been found to exert cardiac protective effects against myocardial ischemia/reperfusion (MI/R) injury, although the detailed mechanisms remain largely unknown. This experiment was performed to explore the potential involvement of silent information regulator 1 (SIRT1)-NFE2-related factor 2 (NRF-2)-heme oxygenase-1 (HO-1) pathway in the cardiac protective actions of punicalagin. Sprague-Dawley (SD) rats were subjected to MI/R operation with or without punicalagin treatment (40 mg kg −1 d −1 ). We showed that punicalagin-treated group exhibited enhanced cardiac function, reduced myocardial infarction and decreased cleaved caspase-3 level. Furthermore, myocardial oxidative/nitrosative stress was ameliorated by punicalagin as evidenced by suppressed superoxide generation, gp91 phox and iNOS expressions, NO metabolites as well as myocardial nitrotyrosine level. Additionally, punicalagin decreased myocardial IL-6, TNF-α and the levels of ICAM-1, VCAM-1 and IKK-β expressions as well as IκB-α phosphorylation and NF-κB nuclear translocation. However, these effects were abolished by EX527 (5 mg kg −1 d −1 , a selective SIRT1 inhibitor). We further found that punicalagin dose-dependently enhanced SIRT1 nuclear distribution and NRF-2-HO-1 signaling. While EX527 treatment not only reduced SIRT1 activity, but also reversed the activation of NRF-2-HO-1 pathway. Collectively, these results revealed that punicalagin reduced cardiac oxidative/nitrosative stress and inflammatory response induced by MI/R operation through SIRT1-mediated activation of NRF-2-HO-1 signaling.
研究领域:
心脏病学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Zekun Dong,Jiao Yang,Jie Yao,Yu Tang,Yan Zhang
Electro-peroxone (EP) is an emerging technology that combines electrochemistry and O 3 to remove contaminants. However, conventional carbon cathodes primarily contribute to the synthesis of H 2 O 2 in the system, which may scavenge HO . Herein, a series of Fe-based layered double hydroxides (LDH) was loaded onto graphite felt cathodes to strengthen the removal of organic contaminants. The EP system with CuFe-LDH/GF cathode (EP-CuFe-LDH/GF) demonstrated the highest removal rate (91.2 % within 20 min) of oxalic acid (OA), a typical ozone-resistance substance, approximately twice that of the EP-GF system. HO was identified the main reactive species. A new pathway for O 2 reduction and the generation of HO was found. The loading of CuFe-LDH altered the reduction pathway of O 2 on the GF cathode from 2e − to 1e − or 4e − process. As a result, HO was produced by the combination of O 3 and O 2 − instead of O 3 and H 2 O 2 , which avoided the quenching of HO by H 2 O 2 and enhanced the removal of OA. Moreover, the breaking complexation by O 3 , cathodic O 3 reduction and metal-OH catalyzed O 3 processes also contributed to the HO formation. Furthermore, the system could efficiently remove OA across a broad pH range of 3.0 to 9.0, addressing the poor performance of conventional EP systems under acidic conditions. Overall, the finding of this significant mechanism advances the understanding of organic pollutants removal and broadens the potential applications of EP system.
研究领域:
环境科学
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电化学工程
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产品关联
DOI
高影响因子
Advanced Optical Materials
·
2024
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IF 8
Shanshan Zhao,Dechao Yu,Benchun Li,Saima Kanwal,Tiantian Shen,Jincheng Wu,Songlin Zhuang,Dawei Zhang
The development of blue/green/red tricolor upconversion (UC) phosphors under invisible light irradiation has attracted significant interest in emerging applications such as anti-counterfeiting, three-dimensional (3D) optical data storage, and solid-state 3D displays. In this study, a series of LiYO 2 :RE 3+ ,Yb 3+ (RE = Tm, Ho, Eu) phosphors are successfully prepared via first precipitation process followed by calcination, and completely optimized for efficient tricolor UC luminescence with 980 nm laser excitation. Mechanisms involving two- or three-photon absorption processes and low-phonon-energy-suppressed multi-phonon relaxation have been proposed for Ho 3+ (Eu 3+ )/Yb 3+ and Tm 3+ /Yb 3+ codoping, respectively. In addition, the RE 3+ (Yb 3+ ) concentration-induced phase transition of the LiYO 2 host is discussed for interesting possibilities in spectral regulation. Based on the broad color gamut achieved by the tricolor UC phosphor mixture, homochromatic and polychromatic metamerisms are experimentally fabricated via screen printing for high-level anti-counterfeiting and information security. Moreover, for a proof-of-concept demonstration, prototypes of 3D optical data storage and volumetric displays are constructed by embedding tricolor UC mixtures and their 3D printed patterns in polydimethylsiloxane. It is believed that the continuous exploration of tricolor UC under invisible light excitation for metamerism anti-counterfeiting and 3D displays can promote the development of high-level information security and advanced volumetric display technologies.
研究领域:
材料科学
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光学工程
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产品关联
DOI
高影响因子
FREE RADICAL BIOLOGY AND MEDICINE
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2026
·
IF 8
Zhan Wang,Cheng Zheng,Wenzhen Li,Kaung Myat Kyaw,Yunmin Zhu,Yihan Lin,Zhen Lin,Di Wu,Zhiqi Hu,Yang Sun,Kaitao Li
Introduction Androgenetic alopecia (AGA), the most common form of hair loss, lacks ideal therapies due to the limitations of current drugs. Natural compounds with low toxicity and good biocompatibility present promising alternatives. Ginkgolide B (GB), a bioactive component of Ginkgo biloba , possesses multiple biological activities, yet its efficacy and mechanism against AGA remain unexplored. Objectives This study aimed to investigate the hair growth-promoting effect of GB in a dihydrotestosterone (DHT)-induced hair loss model and to elucidate the underlying molecular mechanism. Additionally, a novel detachable microneedle delivery system was developed and evaluated to overcome GB's poor transdermal permeability, thereby enhancing its therapeutic efficacy. Methods The AGA microenvironment was first simulated using DHT-stimulated human dermal papilla cells (HDPCs) in vitro. The molecular mechanism by which GB improves the secretory function of HDPCs was investigated through network pharmacology, western blot, transmission electron microscopy, and immunofluorescence. Subsequently, a detachable microneedle system was designed, in which GB was encapsulated in poly (lactic-co-glycolic acid) (PLGA) microspheres and integrated with the sulfobutylether-β-cyclodextrin (SCD)-based matrix (GB@MS-MN). The therapeutic efficacy of this system was evaluated in a DHT-induced hair loss model. Results GB rescued DHT-induced dysfunction in HDPCs, reversing the aberrant secretion of hepatocyte growth factor (HGF) and dickkopf-1 (DKK1). Mechanistically, GB alleviated DHT-induced endoplasmic reticulum stress (ERS) in HDPCs by activating the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway. And GB promoted hair growth in DHT-induced hair loss mice. The GB@MS-MN system demonstrated excellent mechanical properties and targeted follicular delivery and showed superior hair growth promotion compared to free GB and minoxidil. It effectively suppressed follicular ERS, with no observed systemic toxicity. Conclusion In summary, this study confirms that GB protects HDPCs function by suppressing DHT-induced ERS through activation of the Nrf2/HO-1 pathway. Furthermore, a detachable microneedle delivery system based on a PLGA-SCD composite effectively overcomes the transdermal barrier of GB and demonstrates superior hair-promoting efficacy.
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DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2026
·
IF 4
Haixia Han,Juanjuan Gao,Quan Sun,Dandan Tong,Jian Liu,Xiuli Yang,Jingjing Sun,Yue Cui,Juan Wang,Zuoshan Feng,Mingxi Jia
BACKGROUND Acrylamide (ACR), an environmental neurotoxicant prevalent in thermally processed foods, contributes to brain injury via oxidative stress and neuroinflammation. The cumulative effects of long-term low-dose exposure are particularly alarming. As a traditional food ingredient, the inflorescence of Coreopsis tinctoria Nutt. is rich in polyphenolic compounds that exhibit significant anti-inflammatory and antioxidant properties. This study systematically evaluated the neuroprotective potential of Coreopsis tinctoria Nutt. polyphenols (CTNP) against chronic low-dose ACR exposure in mice. RESULTS Behavioral analyses demonstrated that CTNP (0.25–1.00 g kg −1 ) significantly ameliorated ACR-induced gait abnormalities and restored voluntary activity. CTNP mitigated neuronal misalignment and loss of synaptic density in the hippocampal CA1/CA3 regions and markedly reduced serum levels of brain injury markers MBP and GFAP by 30.6% and 41.7%, respectively. Mechanistic investigations revealed that CTNP attenuated oxidative damage via activation of the Nrf2/HO-1 pathway, leading to decreased brain reactive oxygen species and malondialdehyde levels while enhancing catalase, superoxide dismutase and glutathione peroxidase activities. Furthermore, CTNP suppressed MAPK pathway-mediated neuroinflammation, reducing pro-inflammatory factors such as COX-2, TNF- α and IL-1 β . CONCLUSION These findings demonstrate that CTNP, as diet-derived bioactives, mitigate ACR neurotoxicity through coordinated modulation of Nrf2/HO-1 antioxidant and MAPK anti-inflammatory pathways, supporting their potential as a functional food component for preventing environmental toxicant-associated neural damage. © 2026 Society of Chemical Industry.
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DOI
高影响因子
PHYTOMEDICINE
·
2026
·
IF 11.3
Chunge Zhang,Shuai Huang,Wenxin Zhang,Hongbo Teng,Yuna Shao,Yihua Zhang,Shihang Ren,Qi Wu,Yan Zhao,Zhaowei Yan
Background Acanthopanax sessiliflorus ( A. sessiliflorus ) extract has been used in various traditional Chinese medicines and compound preparations for the treatment of arrhythmia. Our preliminary studies have demonstrated that A. sessiliflorus extract exerts significant protective effects against arrhythmia induced by barium chloride. The present study aims to elucidate the bioactive components of A. sessiliflorus and to investigate the underlying mechanisms by which it mitigates barium chloride-induced arrhythmia. Methods Network pharmacology was employed to predict the pharmacological profiles of chemical constituents from A. sessiliflorus, followed by a systematic screening of their biological activities. Subsequently, a barium chloride-induced arrhythmia model was established, and the potential therapeutic mechanisms were analyzed using transcriptomics. Functional and histopathological analyses of the rat hearts were performed. Western blot was used to detect the expression levels of the Nrf2/HO-1 axis, connexins, ion channels, and autophagy-related proteins in myocardial tissue and NRVMs. Subsequently, PCR, immunofluorescence, co-immunoprecipitation, and molecular docking were employed to verify the involvement of the targets in the intervention of arrhythmia. Results The results showed that after treatment with divaroside, the arrhythmia and redox system imbalance induced by BaCl₂ in rats were significantly restored. Divaroside can inhibit calcium overload by regulating the expression levels of RyR2 and SERCA2a in the sarcoplasmic reticulum, serving as a means to alleviate arrhythmia. In addition, we have confirmed that autophagy plays a crucial role in arrhythmia, and divaroside can activate autophagy, thereby protecting cardiomyocytes from BaCl₂-induced damage. Finally, it was confirmed that divaroside can weaken the interaction between the Keap1-Nrf2 complex and promote the nuclear translocation of Nrf2, activating the Nrf2/HO-1 axis and regulating the aforementioned pathways to counteract arrhythmia. Conclusion This study suggests that divaroside prevents the occurrence of arrhythmia by activating autophagy and reducing abnormal calcium ion homeostasis through the Nrf2/HO-1 axis.
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2026
·
IF 6.5
Jiaqi Ding,Xiaolin Xiong,Huanhuan Shi,Yuxiao Mu,Haojun Ji,Jingwen Wang
pH switches reactive species in ZVC/PAA: HO • at pH 3, Cu(III) at pH 7. • Surface Cu x O shell is the key site governing the generation of reactive species. • HO • -dominated system rapidly removes diverse sulfonamides with non-selectivity. • Cu(III) system selectively degrades sulfonamides containing heterocyclic rings. • ZVC demonstrates higher reusability and lower Cu ions leaching at pH 7. Zerovalent copper (ZVC) has garnered considerable attention in Fenton-like reactions, but the surface properties of ZVC that regulate reactive species (RSs) generation remain elusive. Herein, we systematically investigated the pH-dependent regulation of hydroxyl radical (HO • ) and high-valent copper (Cu(III)) generation during peracetic acid (PAA) activation by ZVC for the degradation of sulfonamide antibiotics (SAs). Characterization results revealed that ZVC has a core-shell structure, in which the surface copper oxide (Cu x O) layer serves as the primary active site. Theoretical and experimental analyses elucidated that pH modulates the surface properties of ZVC. At pH 3, the protonation of Cu x O promotes the generation of HO • in the ZVC/PAA system; conversely, under neutral conditions (pH 7.0), the unprotonated state favors the formation of Cu(III) as the dominant RSs. This trade-off between HO • and Cu(III) generation leads to distinct degradation performance for SAs degradation. HO • -dominated system achieved non-selective removal (>96 %) of various SAs; whereas the Cu(III) pathway selectively degraded SAs with heterocyclic rings at pH 7. The Cu(III) pathway outperformed the HO • system in terms of humic acid tolerance and catalyst recyclability in the ZVC/PAA process. This work clarifies the decisive role of surface protonation of ZVC in steering HO • versus Cu(III) pathways and provides a theoretical basis for designing pH‑specific Fenton-like reaction processes. Download: Download high-res image (355KB) Download: Download full-size image
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DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 19
·10889-10896
·
IF 3.049
Xinxin Yan,Tiesheng Li,Linna Guo,Honglei Li,Penglei Chen,Minghua Liu
Pure cubic phase and uniform BiF3:Ln3+ (Ln = Ho, Er, Tm)/Yb3+ nanoparticles (NPs) were prepared by coprecipitation. The growth mechanism of BiF3:2%Er3+/20%Yb3+ NPs was proposed based on evolution analysis of the time-dependent morphology, in which BiF3:2%Er3+/20%Yb3+ was formed through the growth process of “nucleation to crystallization and Ostwald ripening”. The upconversion luminescence (UCL) properties and mechanism of BiF3:Ln3+ (Ln = Ho, Er, Tm)/Yb3+ under dual-wavelength excitation were also systematically investigated. The emission intensity of BiF3:2%Er3+/20%Yb3+ by dual-wavelength excitation (λ = 980 nm + 1550 nm) was 1.49 times more than that excited by 1550 nm or 980 nm individually. Furthermore, the properties of the bright white and multicolor UCL showed that yellow, purple, green, or pinkish light could be observed by controlling the doping concentration of Ln3+ (Ln = Yb, Er, Tm, and Ho), indicating that they had potential applications in backlight sources of color displays and security labeling. The temperature sensitivity of BiF3:2%Er3+/20%Yb3+ exhibited a downward tendency and its max value was about 0.0036 K−1 at 273 K. Cell toxicity tests showed that the UCNPs in phospholipid aqueous solution presented low cytotoxicity. Also, in vivo imaging and X-ray imaging revealed that the BiF3:2%Er3+/20%Yb3+ NPs had deep penetration and high contrast, which meant it could be used as a potential probe and contrast agent in in vivo optical bioimaging.
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DOI
高影响因子
Antioxidants
·
2026
·Vol. 15
·期 9
·1114
·
IF 8.2
Wen-Xu Yao,Yao-Dan Ma,Shi-Yao Ding,Jian Lu,Hao-Lan Tang,Zeng-Ming Yang
Successful embryo implantation requires intimate crosstalk between the blastocyst and the uterine epithelium within a defined window of receptivity. However, the metabolic signals that mediate this process in mammals remain poorly understood. In this study, pregnant mice, primary uterine cell culture and uterine epithelial organoids were used to examine the regulation and function of heme oxygenase-1 (HO-1) during mouse embryo implantation and decidualization. We demonstrate that embryo-derived lactic acid drives heme catabolism and regulates epithelial receptivity in mice through a hypoxia-inducible factor 1α (HIF1α) -heme oxygenase-1 (HO-1) signaling axis. Specifically, lactic acid stabilizes HIF1α to induce HO-1 expression in uterine epithelial cells by promoting von Hippel-Lindau (VHL) nucleolar sequestration and downregulating PHD2/3. Additionally, lactic acid suppresses the transcriptional repressor BACH1, further facilitating HO-1 induction. At physiological heme levels, HO-1-derived bilirubin promotes epithelial receptivity by increasing phosphorylated STAT3 (p-STAT3) and downregulating MUC1. A low dose of hemin promotes epithelial receptivity and decidualization, whereas a high dose of hemin suppresses these processes. Pharmacological inhibition of HO-1 in mice markedly reduces implantation sites, establishing the functional necessity of this pathway. However, when heme levels exceed the regulatory capacity of HO-1, epithelial dysfunction ensues, characterized by reduced p-STAT3 and elevated MUC1, which ultimately disrupts implantation. Consistent with this, chronic heme exposure by oral gavage in mice increases uterine heme levels and upregulates BACH1, thereby suppressing HO-1 and trapping the uterus in a non-receptive state, causing implantation failure. Our findings define a lactic acid-HIF1α-HO-1-heme metabolic checkpoint that couples glycolytic signaling to heme regulation and endometrial receptivity. Dysregulation of this checkpoint may contribute to implantation disorders associated with heme stress, providing mechanistic insights into heme stress-related uterine receptivity failure.
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高影响因子
CERAMICS INTERNATIONAL
·
2022
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IF 5.532
Zhiqiang Li,Shiguang Yan,Jiake Xia,Fei Cao,Xuefeng Chen,YunXiong Song,Yonghong Chen,Zhi-Sheng Lin,Genshui Wang
With the increasing demand for the miniaturization, high capacitance and high reliability of the Multilayer Ceramic Capacitor (MLCC), the thickness of single dielectric layer is less than 1 μm and the original partical size of the powder has reached the scale less than 300 nm. In this paper, BaTiO 3 powder of various partical sizes (150–500 nm) was modified with Dy and Ho through a chemical coating method. The dense and uniform ceramics in accordance with EIA X7R-type temperature stability were sintered under low oxygen partial pressure and good dielectric properties of dielectric constant >2000 and dielectric loss <1% were obtained. The grain size effect on the dielectric properties under various dc-bias field were systematically investigated. It is found that the fine-grain ceramic exhibits a lower absolute change of the dielectric constant and a higher dc-bias stability. Furthermore, the modified 150 nm BaTiO 3 powders was made into 1210-type MLCCs, and the dielectric behavior of the MLCCs with different dc-fields and ac-fields were study over −55 °C–125 °C. It is found that the ac electric field had a greater influence on the dielectric properties at room temperature and below, and the dielectric constant variation under a dc-bias field was also greatly advanced for the modified fine-grain MLCCs. The results provide a reference for the design of the next generation of BME-MLCCs.
研究领域:
材料科学
·
电子工程
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DOI
高影响因子
危险品化学品经营许可证（带存储）