JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Hui Feng,Meng Luo,Guonian Zhu,Kerage Dorothy Mokeira,Yaoxin Yang,Yongxin Lv,Qing Tan,Xiangwen Lei,Hang Zeng,Hefa Cheng,Shuxia Xu
This study described the development of a photocatalytic electrochemical aptasensor for the purpose of detecting chloramphenicol (CAP) antibiotic residues in water by utilizing SYBR Green I (SG) and chemically exfoliated MoS 2 (ce-MoS 2 ) as synergistically signal-amplification platforms. The Au nanoparticles (AuNPs) were electrodeposited onto the surface of an indium tin oxide (ITO) electrode. After that, the thiolate-modified cDNA, also known as capture DNA, was combined with the aptamer. Subsequently, photosensitized SG molecules and ce-MoS 2 nanomaterial were inserted into the groove of the resultant double-stranded DNA (dsDNA). The activation of the photocatalytic process upon exposure to light resulted in the generation of singlet oxygen. This singlet oxygen effectively splits the double-stranded DNA, resulting in a significant enhancement in the current of [Fe(CN) 6 ] 3-/4- . When the CAP was present, both SG molecules and ce-MoS 2 broke away from the double-stranded DNA, which turned off the photosensitization response, leading to a significant decrease in the current of [Fe(CN) 6 ] 3-/4- . Under optimal conditions, the aptasensor exhibited a linear relationship between CAP and logarithmic concentrations from 20 to 1000 nM, with a detection limit (3 σ ) of 3.391 nM. The aptasensor also demonstrated good selectivity towards CAP in the presence of interfering antibiotics such as tetracycline, streptomycin, levofloxacin, ciprofloxacin, and sulfadimethoxine. Additionally, the results obtained from the analysis of natural water samples using the proposed aptasensor were consistent with the findings acquired through the use of a liquid chromatograph-mass spectrometer. Therefore, with its simplicity and high sensitivity, this biosensor can potentially detect alternative antibiotics in environmental water samples by replacing the aptamers based on photosensitization.
研究领域:
环境科学
·
分析化学
·
生物传感器
·
水质监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Inorganic and Nano-Metal Chemistry
·
2019
·
IF 0.685
Xue-Wen Liu,Jie Huang,Yu-Xuan Tang,Song-Bai Zhang,Ji-Lin Lu
Two new ruthenium complexes containing a new polyaza ligand were found to inhibit the DNA supercoiled relaxation mediated by topoisomerase I (topo I), cleave DNA under irradiation and interact with CT-DNA by intercalation. Both complexes exhibited fast singlet oxygen generation rate, and DNA photocleavage reaction should be initiated by 1O2. Furthermore, complex 2 showed higher photocleavage activity, topo I inhibition activity, and DNA affinity than complex 1. This work shows that two complexes can act as DNA binders with high affinity and good DNA photocleavers. Furthermore, complex 2 can act as a good topo I inhibitor.
研究领域:
化学
·
分子生物学
·
药理学
✓ 已验证
DOI
高影响因子
Micromachines
·
2025
·Vol. 16
·期 3
·270
·
IF 3
Jiangbo Wu,Zihan Yan,Yongqing He,Jie Liu,Yao Lv
Deterministic lateral displacement (DLD) is a passive particle separation method based on microfluidic technology, with its separation mechanism primarily relying on particle size differences. Therefore, the critical separation size is of great significance in the design of DLD devices. The geometric asymmetry of the pillar array design significantly influences fluid behavior and critical particle size variations. This study first analyzed particle motion characteristics through particle trajectory observation experiments within asymmetric microfluidic chips. Subsequently, a two-dimensional numerical simulation method was employed to investigate the effects of three different ratios of lateral gap size to downstream gap size (Gx:Gy) on particle trajectories and flow field distribution. The results indicate that as Gx:Gydecreases, the upward flow rate gradually reduces, accompanied by changes in the flow field velocity distribution, causing particles to favor displacement mode. This study provides new theoretical foundations for the precise regulation of particle motion behavior and introduces novel insights for optimizing DLD device design.
研究领域:
生物工程
·
微流控技术
·
颗粒分离
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INORGANICA CHIMICA ACTA
·
2022
·
IF 3.118
Xin Yan,Jia-Qian Chen,Mao-Lin Hu,Hiroshi Sakiyama,Mohd. Muddassir,Jian-Qiang Liu
Two different silver(I) complexes with diphenylphosphonic acid as peripheral ligand were designed and synthesized by using different solvents. The complexes were characterized by X-ray photoelectron spectroscopy (XPS), infrared spectroscopy (IR), elemental analysis (EA), thermogravimetric analysis (TG). Single crystal X-ray analysis established the final structure of both complexes. The interaction modes of the two clusters with DNA were investigated by fluorescence and electrochemical analysis. The theoretical result was justified with the help of theoretical data. The results established that the cluster with methanol solvent was more stable binding with the DNA and protein than the cluster with DMF molecule.
研究领域:
生物无机化学
·
配位化学
·
生物物理学
✓ 已验证
DOI
高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2018
·Vol. 32
·期 8
·e4423
·
IF 3.581
XueWen Liu,Jie Huang,YuXuan Tang,YouMing Shen,JiLin Lu
A new polypyridyl ligand containing a nitro group and two new ruthenium complexes of it were synthesized. The two complexes exhibited non-dppz DNA ‘light switch’ effects after interaction with calf thymus DNA. Introducing both electron-withdrawing group (─ NO 2 ) and electron-donating group (─ CH 3 ) may be the reason that the two complexes display DNA ‘light switch’ behaviors. Furthermore, one of the complexes showed higher photocleavage activity, topoisomerase I inhibition activity and DNA affinity than the other. The present work shows that the more active complex can be employed as a non-dppz DNA ‘light switch’, DNA photocleaver and topoisomerase I inhibitor. In addition, the two complexes have no or weak cytotoxic activities against Eca-109 and A549 cells.
研究领域:
化学
·
生物化学
·
分子生物学
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 9
·3325-3332
·
IF 4.58
Ji Danyang,Meng Hongmin,Ge Jia,Zhang Lin,Wang Hongqi,Bai Dongmei,Li Jianjun,Qu Lingbo,Li Zhaohui
The authors describe a silver(I) mediated fluorescent assay for glutathione (GSH). An allosteric oligonucleotide strand containing a G-rich sequence is used to produce a G-quadruplex, and N-methylmesoporphyrin IX (NMM) is chosen as the fluorescent probe. In the absence of Ag(I), the DNA strand is partially intramolecularly hybridized to form a hairpin structure wherein the G-rich sequence is partially caged. On addition of Ag(I), the hairpin is disrupted by forming C-Ag(I)-C base pairs. As a result, the G-rich sequence is released and folds into a G-quadruplex structure, which is able to bind NMM to generate strong fluorescence at 612 nm. However, in the presence of GSH, due to the strong binding ability between GSH and Ag(I), the C-Ag(I)-C structure is not formed. Hence, the DNA probe reverts back to its original structure and fluorescence is not increased. Based on these findings, a method was worked out that has a detection limit as low as 3.5 nM. Due to the inherent selectivity of the interaction between GSH and Ag(I), the method is highly selective over common potential interfering species. It was successfully applied to the fluorometric determination of GSH in cell extracts.
Schematic of a highly sensitive fluorometric glutathione (GSH) assay based on a conformational switch of a G-quadruplex mediated by silver(I) and N-methylmesoporphyrin IX (NMM) as a signal reporter.
研究领域:
分析化学
·
生物化学
·
分子生物学
✓ 已验证
DOI
高影响因子
Advanced Science
·
2024
·
IF 14.3
Zhen Tong,Jia-Peng Zou,Su-Yun Wang,Wei-Wei Luo,Yan-Yi Wang
Interferons (IFNs) activate JAK-STAT pathways to induce downstream effector genes for host defense against invaded pathogens and tumors. Here both type I (β) and II (γ) IFNs are shown that can activate the transcription factor IRF3 in parallel with STAT1. IRF3-deficiency impairs transcription of a subset of downstream effector genes induced by IFN-β and IFN-γ. Mechanistically, IFN-induced activation of IRF3 is dependent on the cGAS-STING-TBK1 axis. Both IFN-β and IFN-γ cause mitochondrial DNA release into the cytosol. In addition, IFNs induce JAK1-mediated tyrosine phosphorylation of cGAS at Y214/Y215, which is essential for its DNA binding activity and signaling. Furthermore, deficiency of cGAS, STING, or IRF3 impairs IFN-β- or IFN-γ-mediated antiviral and antitumor activities. The findings reveal a novel IRF3 activation pathway parallel with the canonical STAT1/2 activation pathways triggered by IFNs and provide an explanation for the pleiotropic roles of the cGAS-STING-IRF3 axis in host defense.
研究领域:
免疫学
·
分子生物学
·
微生物学
·
癌症研究
✓ 已验证
DOI
PubMed
高影响因子
Cell Reports
·
2025
·
IF 6.9
Li Chai,Chaoqun Li,Xuefeng Wang,Yanyang Qin,Hemin Sun,Jie Du,Liu Yang,Dongmei Hu,Jianan Xiong,Zhiyuan Zhao,Rong Gong,Tao Wu,Meng Wu,Meng Nie,Jialin Gao,Jihui Jia,Chengjiang Gao,Wei Zhao,Huan Zhou,Dongwei Kang,Mutian Jia
Perturbation of energy metabolism is an essential feature during infection and inflammation. TANK-binding kinase 1 (TBK1) is crucial for initiating the innate immune response against viral infection, although aberrant and ongoing TBK1 activation induces excessive production of type I interferons (IFN-I). Nonetheless, the mechanisms whereby energy metabolism controls TBK1 activation remain unclear. Here, we elucidate a mechanism linking energy metabolism to the inhibition of TBK1-induced IFN-I responses via the immune response gene 1 (IRG1)-itaconic acid axis. Mechanistically, itaconic acid and its derivatives alkylated TBK1 at Cys605, thereby disrupting TBK1 dimerization and rapid activation. IRG1, the enzyme that catalyzes itaconic acid production, is upregulated during late-phase viral infection and acts as a feedback regulator to restrain TBK1 activity. We developed itaconic acid-based compounds ITA-5/ITA-9 as alternative TBK1 inhibitors. ITA-5/ITA-9 effectively limited excess IFN-I-mediated hyperinflammation. These findings provide a promising therapeutic strategy for treating diseases mediated by aberrant TBK1 activation.
研究领域:
免疫学
·
分子生物学
·
代谢调控
·
先天免疫
·
病毒感染
·
信号转导
·
药物开发
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Zhishuang Yang,Tianjing Lan,Hongyan Luo,Pei Li,Mingshu Wang,Renyong Jia,Shun Chen,Mafeng Liu,Xinxin Zhao,Qiao Yang,Ying Wu,Shaqiu Zhang,Juan Huang,Xumin Ou,Sai Mao,Qun Gao,Di Sun,Bin Tian,Anchun Cheng,Dekang Zhu
Antimicrobial resistance remains an utmost concern in human and veterinary medicine, impacting humans, animals, and the environment while significantly influencing the principles of One Health. While Riemerella anatipestifer ( R. anatipestifer ) is recognized as a waterfowl pathogen with multidrug-resistant properties, the specifics of its lincosamide resistance mechanism are inadequately understood. In this study, we identified a novel lincosamide resistance gene, lnu (I), in R. anatipestifer RCAD0121, and investigated its potential origin, transfer mechanisms, and dissemination status through genomic epidemiology. This exhibited 74.80 % amino acid identity with a previously reported gene, lnu (H). PCR analysis revealed lnu (I) prevalence in at least 44 R. anatipestifer isolates collected from multiple provinces in China. Furthermore, genomic mining unveiled 56 lnu (I) sequences within publicly available databases, primarily originating from environmental sources. In addition, members of the family Flavobacteriaceae were the dominant (16/56, 28.57 %) bacteria carrying the lnu (I) gene, with Flavobacterium exhibiting a similar GC content as lnu (I). Notably, specific instances of the lnu (I) gene were linked to mobile genetic elements within human and animal pathogenic bacteria. These findings suggest that Flavobacterium species within the environment could serve as potential ancestral sources of the novel lnu (I) gene, which has undergone mobilization events toward pathogenic bacteria.
研究领域:
微生物学
·
分子生物学
·
医学
·
基因组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2017
·
IF 4.291
Chen Guilin,Guo Mingquan
Topoisomerase I (Topo I) catalyzes topological interconversion of duplex DNA during DNA replication and transcription, and has been deemed as important antineoplastic targets. In this study, the fraction R.d-60 from ethyl acetate extracts of Rhamnus davurica showed higher inhibitory rates against SGC-7901 and HT-29 compared with the R.d-30 fraction in vitro. However, the specific active components of R.d-60 fraction remain elusive. To this end, a method based on bio-affinity ultrafiltration and high performance liquid chromatography/electrospray mass spectrometry (HPLC- ESI-MS/MS) was developed to rapidly screen and identify the Topo I inhibitors in this fraction. The enrichment factors (EFs) were calculated to evaluate the binding affinities between the bioactive constituents and Topo I. As a result, eight ligands were identified and six of which with higher EFs showed more potential antitumor activity. Furthermore, antiproliferative assays in vitro (IC50 values) with two representative candidates (apigenin, quercetin) against SGC-7901, HT-29 and Hep G2 cells were conducted and further validated. Finally, the structure-activity relationships revealed that flavones contain a C2-C3 double bond of C ring exhibited higher bio-affinities to Topo I than those without it. This integrated method combining Topo I ultrafiltration with HPLC-MS/MS proved to be very efficient in rapid screening and identification of potential Topo I inhibitors from the complex extracts of medicinal plants, and could be further explored as a valuable high-throughput screening platform in the early drug discovery stage.
研究领域:
药理学
·
分析化学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COORDINATION CHEMISTRY
·
2022
·
IF 1.869
Chengjie Gao,Zhenzhou Sun,Ning Zhu,Hongliang Han,Zhongfeng Li,Chaoyue Gu,Yuping Yang,Xiulan Xin,Qiming Qiu,Wei Yang,Guo Wang,Qionghua Jin
Two new complexes, [Cu(dmp)(2-bdppmapy)](CF3SO3)·CH2Cl2 (1) and [Cu(imidazo[4,5-f])(2-bdppmapy)](SCN) (2) (2-bdppmapy = N,N-bis[(diphenylphosphino)methyl]-2-pyridinamine, dmp = 2,9-dimethyl-(1,10)phenanthroline and imidazo[4,5-f] = 1H-imidazo[4,5f][1,10]phenanthroline), have been synthesized in mixed solvents of CH3OH and CH2Cl2 (1:1, V/V). On the basis of studying the structure and photophysical properties of the Cu(I) complexes, the effects of different N ligands and counter anions on the luminescence properties of the Cu(I) complexes were characterized by single-crystal X-ray diffraction, fluorescence spectra and terahertz time-domain spectroscopy (THz-TDS). The research shows that 1 exhibits excellent photoluminescence quantum yields. It is ascribed to the unique stacked structure. In addition, the relationship between the multiple weak forces, structure, and photophysical properties of the complexes was investigated.
研究领域:
化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Omega
·
2020
·
IF 2.87
Yan Ji,Xiaoliang Yang,Zhi Ji,Linhui Zhu,Nana Ma,Dejun Chen,Xianbin Jia,Junming Tang,Yilin Cao
The infrared spectrum (IR) characteristic peaks of amide I, amide II, and amide III bands are marked as amide or peptide characteristic peaks. Through the nuclear magnetic resonance study, N-methylacetamide has been determined to have six fine components, which include protonation, hydration, and hydroxy structures. Then the independent IR spectrum of every component in N-methylacetamide is calculated by using the density functional theory quantum chemistry method, and the contribution of each component to amide I, II, and III bands is analyzed. The results of this research can help to explain the formation of the amide infrared spectrum, which has positive significance in organic chemistry, analytical chemistry, and chemical biology.
研究领域:
有机化学
·
分析化学
·
化学生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）