JOURNAL OF LIPID RESEARCH
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2021
·Vol. 63
·期 1
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IF 5.922
Rui Peng,Juan Le,Shu-Lin Yang,Jing-Ru Cheng,Yan Li,Shao-Ting Wang
Sex hormones, including androgens, estrogens, and progestogens, are important biomarkers for various diseases. Quantification of sex hormones is typically conducted by LC-MS/MS. At present, most methods require liquid-liquid extraction or solid phase extraction for sample preparation. However, these pretreatments are prone to compromise LC-MS/MS throughput. To improve on the current standard practices, we investigated cold-induced phase separation for sex hormone extraction. After protein precipitation with acetonitrile and adjusting the solution constitution with water, samples were stored at −30°C for 10 min to generate two distinct phases: an acetonitrile-rich layer on top of a water-rich layer. During this process, the hydrophobic sex hormones spontaneously separate into the upper layer. This simple and reliable cold-induced phase separation-based LC-MS/MS methodology was used here to simultaneously detect estrone, estradiol, estriol, testosterone, androstenedione, dehydroepiandrosterone, progesterone, and 17-hydroxyprogesterone in serum. Validation of this method indicated satisfactory performance, including acceptable linearity, accuracy, precision, and tractability. Compared with the mainstream liquid-liquid extraction-based method, this new method exhibits significant progress in throughput, which shortens the time cost of sample preparation from 90 to 40 min. We propose that this method can be an excellent alternative for sex hormone analysis in routine clinical laboratories.
研究领域:
医学检验
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分析化学
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内分泌学
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PubMed
高影响因子
Metabolites
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2022
·Vol. 12
·期 9
·841
·
IF 5.581
Simeng Zhang,Yuchan Deng,Annan Zhang,Lili Yan,Zhichao Zhang,Jing Wei,Qiang Zhang
The transitional expression and aggregation of amyloid β (Aβ) are the most important causative factors leading to the deterioration of Alzheimer’s disease (AD), a commonly occurring metabolic disease among older people. Antioxidant agents such as vitamin C (Vc) have shown potential effects against AD and aging. We applied an liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method and differential metabolites strategy to explore the metabolic disorders and Vc restoration in a human Aβ transgenic (Punc-54::Aβ1–42) nematode model CL2006. We combined the LC-MS/MS investigation with the KEGG and HMDB databases and the CFM-ID machine-learning model to identify and qualify the metabolites with important physiological roles. The differential metabolites responding to Aβ activation and Vc treatment were filtered out and submitted to enrichment analysis. The enrichment showed that Aβ mainly caused abnormal biosynthesis and metabolism pathways of phenylalanine, tyrosine and tryptophan biosynthesis, as well as arginine and proline metabolism. Vc reversed the abnormally changed metabolites tryptophan, anthranilate, indole and indole-3-acetaldehyde. Vc restoration affected the tryptophan metabolism and the biosynthesis of phenylalanine, tyrosine and tryptophan. Our findings provide supporting evidence for understanding the metabolic abnormalities in neurodegenerative diseases and the repairing effect of drug interventions.Keywords:Alzheimer’s disease;dementia;neurodegenerative disease;aging;Aβ;metabolism;amino acid;ascorbic acid
研究领域:
神经科学
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代谢组学
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药理学
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生物化学
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PubMed
高影响因子
FOOD CHEMISTRY
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2023
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IF 8.8
Zhixin Fei,Mingyue Sun,Qing Song,Chengxi Li,Yang Liu
For the first time, a simple, quick, sensitive, and low cost method for quantification of perchlorate in honey using liquid chromatography-tandem mass spectrometry was developed. Through freezing-assisted sugaring-out liquid–liquid extraction, one-step simultaneous extraction and clean-up of perchlorate from honey were perfectly achieved. Glucose and fructose, the most abundant sugars in honey, were almost completely removed from the extract without use of any clean-up materials. Under optimum conditions, the proposed approach exhibited satisfactory linearity, negligible matrix effects, and low detection limit of 0.05 µg/kg, providing recoveries of 96.7 %–102.3 % with relative standard deviation of < 9 % for honey samples. The validated method was applied to the analysis of perchlorate in 36 honey samples, and detection rate was 94.4 %. This work provided a simple and reliable method for extensive monitoring of perchlorate in honey and opened- up new insights for analysis of contaminants in honey matrixes.
研究领域:
食品科学
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环境科学
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PubMed
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Li Liang,Jincheng Zhang,Baoguo Sun,Ming Zhang,Yan Zhang,Ku Li,Yuyu Zhang
Hypertension has emerged as a major factor detrimental to human health. Umami peptide IIVFGRQLL from yeast has a certain ACE inhibitory activity (IC 50 : 59.35 μM) at 0.14 mg/mL. Moreover, IIVFGRQLL could regulate the systolic blood pressure of spontaneously hypertensive rats model indicating its potential antihypertensive effect. The changes in ACE inhibitory activity and peptide sequence during the digestion were investigated based on the ACE inhibition assays, simulated gastrointestinal digestion, and LC-MS/MS. The ACE inhibitory activity of IIVFGRQLL decreased by 39.0% after digestion. It was hydrolyzed into 5 peptide fragments, among which IVF took the biggest share followed by IVFG, VF, FGR, and RQL. The inhibitory rate of IVF (78.70%), FGR (86.30%), and VF (83.50%) was higher than that of IIVFGRQLL (60.11%). The molecular docking results revealed all the peptides interacted with active residues related to Zn 2+ and S1 pocket in ACE. Besides, IVF, FGR and RQL showed increased electrostatic interaction with Zn 2+ . These results provide theoretical support for the future exploration of umami peptide as a food additive or seasoning to alleviate hypertension.
研究领域:
药理学
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食品科学
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营养学
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生物化学
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高影响因子
Scientific Reports
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2015
·Vol. 5
·期 1
·1-11
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IF 5.578
Yang Ting,Xu Feifei,Fang Danjun,Chen Yun
The distinct roles of protein isoforms in cancer are becoming increasingly evident. FRα and FRβ, two major isoforms of the folate receptor family, generally have different cellular distribution and tissue specificity. However, the presence of FRβ in breast tumors, where FRα is normally expressed, complicates this situation. Prior to applying any FR isoform-based diagnosis and therapeutics, it is essential to monitor the expression profile of FR isoforms in a more accurate manner. An LC-MS/MS-based targeted proteomics assay was developed and validated in this study because of the lack of suitable methodology for the simultaneous and specific measurement of highly homologous isoforms occurring at low concentrations. FRα and FRβ monitoring was achieved by measuring their surrogate isoform-specific peptides. Five human breast cell lines, isolated macrophages and 60 matched pairs of breast tissue samples were subjected to the analysis. The results indicated that FRβ was overexpressed in tumor-associated macrophages (TAMs) but not epithelial cells, in addition to an enhanced level of FRα in breast cancer cells and tissue samples. Moreover, the levels of the FR isoforms were evaluated according to the histology, histopathological features and molecular subtypes of breast cancer. Several positive associations with PR/ER and HER2 status and metastasis were revealed.
研究领域:
癌症生物学
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蛋白质组学
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临床肿瘤学
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PubMed
高影响因子
Food Bioscience
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2024
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IF 4.8
Qi Sun,Guangqing Mu,Peng Sun,Qing Zhao,Xiaomeng Wu,Fanhua Kong
Casein (CN) is the primary factor leading to allergic reactions in infants to bovine milk, severely limiting the intake of bovine milk formula by allergic infants. This study investigates the conformational changes and regulation of linear epitopes during the hydrolysis of CN by five proteases derived from microbial and plant sources using multiple spectroscopic techniques combined with LC/MS-MS. The results showed that the IgE inhibition rates for SD (subtilisin hydrolysate), PD (papain hydrolysate), BD (bromelain hydrolysate), KD (proteinase K hydrolysate), and FD (ficin hydrolysate) were 43.19%, 41.99%, 35.45%, 48.15%, and 14.80%, respectively. The differences in antigenic residues remaining after hydrolysis by different proteases mainly result from the varying degrees of structural alteration of CN and the hydrolysis allergic epitope regions. This study provides theoretical basis for understanding the mechanism by which proteases reduce the allergenicity of CN. It also offers new insights into the development of multienzyme combinatorial hydrolysis strategies.
研究领域:
食品科学
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免疫学
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生物化学
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高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2024
·
IF 3.1
Xina Yu,Shanshan Song,Zhanhua Li,Tiantian Wang,Hui Huang,Qing Shen,Zongyuan Wu,Pei Luo
Panaxynol is a bioactive polyacetylene in food plants; however, its specific benefits in diabetes and metabolic disorders remain unclear. Previous studies have mainly focused on biochemical indicators and clinical evaluations. Limited research has systematically elucidated the beneficial effects of panaxynol from the oxylipins perspective. In this study, we employed an oxylipin analysis platform we previously established using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) based on the multiple reaction monitoring (MRM) method for the profiling of oxylipins. After a 7-week administration of panaxynol to db/db mice, significant alterations in serum oxylipins and potential benefits to hyperglycemia, insulin resistance, and hepatic steatosis were observed. Our analysis also revealed correlations among epoxygenase products derived from arachidonic acid (AA), linoleic acid (LA), and α-linolenic acid (ALA) via cytochrome P450 (CYP) pathways. Furthermore, six potential oxylipins were identified, as offering insights into the mechanisms by which panaxynol may modulate diabetes. These results provide the first in vivo evidence of the impact of panaxynol on oxylipin metabolism and lay the foundation for developing panaxynol as a nutraceutical for diabetes management.
研究领域:
代谢组学
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营养学
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药理学
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PubMed
高影响因子
Food Bioscience
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2024
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IF 4.8
Cheng Yang,Yan Wang,Pei-Zhi Dong,Yanan Li,Yue-Hong Pang
Bisphenols (BPs) pose a pervasive threat to human health due to their potential to leach from plastic packaging into food, thereby endangering the endocrine system. Given their low concentrations in complex samples, the development of a swift, efficient pretreatment method is imperative. In this study, a magnetic covalent organic framework (Fe 3 O 4 @TzDa) was synthesized via a layer-by-layer growth approach, utilized in rapid magnetic solid-phase extraction (MSPE) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the determination BPs. This method exhibited remarkable sensitivity, detecting five BPs (BPS, BPF, BPA, BPB, and BPAF) at levels between 0.68 and 0.77 μg L −1 . Fe 3 O 4 @TzDa nanocomposites maintained an extraction efficiency of approximately 98.0% over five cycles, ensuring robust and repeatable performance. Sample analysis, encompassing plastic bottled water, pear juice, mix juices, pop-top canned curry beef, and box-packed milk, yielded recoveries of 72.0%–114.0% (RSDs <7.5%, n = 5). Furthermore, BPs migration in sports cups and portable bottles was evaluated, revealing migration levels ranging from 0.0014 to 0.0058 mg kg −1 . This study advances BP analysis by introducing a reliable method for their efficient determination in beverage samples from plastic food packages. The utilization of Fe 3 O 4 @TzDa underscores a pivotal advancement in sample preparation, enhancing both sensitivity and efficiency for the extraction and determination of BPs in food samples.
研究领域:
食品安全
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分析化学
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环境科学
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高影响因子
JOURNAL OF SEPARATION SCIENCE
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2021
·Vol. 44
·期 20
·3799-3809
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IF 3.645
Xu-zhao Li,Shuai-nan Zhang,Qi Liu,Wu-de Yang,Ying Zhou
Herba Lysimachiae inhibits synovial damage in osteoarthritis via regulating two bio labels (integrin alpha 2b/beta 3). However, the relevant active ingredients are still unknown. Here, the active ingredients of herbal medicines were analyzed based on the liquid chromatography–tandem mass spectrometry technology and public bioinformatics platforms. The liquid chromatography–tandem mass spectrometry technology was used for compound analysis, and public databases (PubChem BioAssay and STRING) were applied to establish the links between herbal compounds and both bio labels, and identify which herbal compounds may regulate these bio labels. Subsequently, the osteoarthritis model was used to confirm the results. Totally, ninety compounds in Herba Lysimachiae were identified based on the liquid chromatography–tandem mass spectrometry technology. Bioinformatics analysis showed that five compounds (myricetin, fisetin, esculetin, 7-hydroxycoumarin-4-acetic acid, and caffeic acid) may synergistically regulate bio labels through 11 targets, which may be the active ingredients of Herba Lysimachiae for osteoarthritis treatment. In the verification experiments, five compounds markedly suppressed the overexpression of bio labels in the synovium of the osteoarthritis model. In conclusion, the present study effectively and rapidly analyzed the active ingredients of Herba Lysimachiae for osteoarthritis treatment.
研究领域:
药理学
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生物信息学
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分析化学
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风湿病学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2026
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IF 5.2
Liang Li,Wanting Gao,Xinyan Chen,Meijia Liu,Wei Long,Qunyin Zhu
NH₂-MCOF enables ultra-sensitive detection of 17 PFASs down to 0.0346 ng/L. • Fast MSPE within 40 min and reusable for ≥6 cycles with stable performance. • Successfully detected PFASs in real urban waters, including 3.346 μg/L PFDS. Per - and polyfluoroalkyl substances (PFAS), as a class of persistent organic pollutants, are characterized by strong environmental persistence and high bioaccumulation potential, making them a major concern in global environmental and health fields. Currently, the trace detection of PFASs in urban environmental water samples, including surface water, source water, and domestic wastewater faces challenges such as insufficient adsorption selectivity of traditional materials and cumbersome pretreatment steps, highlighting the urgent need to develop efficient enrichment and highly sensitive analytical methods. To improve the enrichment efficiency of PFAS, this study designed and synthesized an amino-functionalized magnetic covalent organic framework material (NH₂-MCOF) as an adsorbent. Combined with magnetic solid-phase extraction (MSPE) and liquid chromatography-tandem mass spectrometry, a synchronous analytical method was established for the determination of 17 PFASs in real urban environmental water matrices. Through systematic optimization, the optimal pretreatment conditions were determined as follows: adsorbent dosage of 20 mg, solution pH of 3.0, ultrasonic-assisted adsorption time of 40 min, and a stepwise elution strategy using methanol, ammoniated methanol, and formic acid-modified methanol. Under the optimized conditions, the method demonstrated good linearity within the range of 0.5–200 μg/L, with detection limits of 0.0346–1.30 ng/L. The spiked recoveries ranged from 50% to 138%, and the intra -day and inter -day precision, expressed as relative standard deviations (RSD), were below 13.5% and 14.9%, respectively. Application to real water samples confirmed the method's effectiveness in detecting multiple PFASs, demonstrating its practicality and reliability. The analytical method established in this study integrates functionalized material design, efficient pretreatment, and sensitive detection, providing a reliable approach for the synchronous monitoring of multiple trace PFAS in real urban environmental water matrices and highlighting the potential application of functionalized magnetic covalent organic framework materials in environmental analysis. Download: Download high-res image (103KB) Download: Download full-size image
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2026
·
IF 2.5
Yingxiao Han,Songwei Ma,Rui Ma,Jingjing Li,Lei Qiu,Jia wen Ma,Yanyan Ma,Mengyin Tang,Zhijuan Cao,Ziqi Li,Shulin zhang,Guorong Ma
Mycobacterium tuberculosis culture filtrate proteins (MTB-CFPs) are critical for tuberculosis diagnosis and vaccine development. To explore their diagnostic potential, MTB was statically cultured in Sauton's medium for 15 days; MTB-CFPs were collected, fractionated using six biomimetic affinity chromatography (BiAC) resins, and analyzed by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). Four MTB-specific regions of difference (RDs) and the PstS1 protein were recombinantly expressed and purified, with their serological efficacy assessed by ELISA using sera from 157 pulmonary tuberculosis (pTB) patients and 71 healthy controls (HCs). LC-MS/MS identified 622 MTB proteins, 307 of which were validated with ≥2 peptides and P < 0.05. BiAC fractionation significantly increased the average peptide number (2.09-fold), peptide coverage (1.43-fold), and the exponentially modified Protein Abundance Index (emPAI) (1.70-fold) in 281 common identifications. Receiver Operating Characteristic (ROC) analysis revealed rEC (a fusion expression product of the Rv3875 and Rv3874 genes in the RD1 region, linked by a 15-nucleotide connector) as the optimal diagnostic marker Area Under the Curve (AUC = 0.8030), with sharply distinct IgG levels between pTB patients and HCs ( P < 0.0001). Notably, rEC combined with GmdA (encoded by the Rv1511 gene located in RD11) enhanced sensitivity while preserving high specificity, especially for Acid-Fast Bacilli (AFB) negative pTB cases demonstrating substantial clinical translation potential. Additionally, extended culture promoted MTB autolysis and cytoplasmic protein release, providing valuable insights into TB pathogenesis and biomarker discovery. This integrated approach of BiAC fractionation, proteomics, and serological validation advances TB precision diagnostics.
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PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
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IF 4.1
Yang Shuang,Wan Wei,Qu Ziyu,Yi Keke,Zhang Di,Jiang You,Ye Zihong,Fang Xiang,Xie Jie,Dai Xinhua
Steroid hormones play crucial regulatory roles in human growth, development, reproduction, and the maintenance of internal environmental homeostasis. Because the concentrations of most hormones in the body are extremely low and their chemical properties vary widely, developing an accurate and sensitive quantitative method has become a primary objective in clinical diagnostics. In this study, an isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) method was developed and validated for the simultaneous quantification of 22 steroid hormones in human serum. To enhance extraction and purification efficiency, a protein precipitation–solid-phase extraction (PP-SPE) approach was employed, forming a “dual purification” strategy. The limits of quantification for progesterone, cortisol, dihydrotestosterone, and dehydroepiandrosterone sulfate were 0.05, 0.50, 0.05, and 1.00 ng/mL, respectively, representing an improvement over previously reported values obtained using similar sample pretreatment methods. Method validation results demonstrated strong linearity for all 22 steroid hormones ( R 2 > 0.98). Accuracy ranged from 86.9% to 119.5%, intra-day precision was below 9.7%, inter-day precision was below 12.0%, and the matrix effect was controlled within 80.3%–118.1%. These findings indicate that the method is accurate, reliable, and suitable for analyzing multiple components in complex serum matrices. Twenty real serum samples were analyzed to evaluate the clinical applicability of the method. The results demonstrated that this method can be used clinically to monitor steroid hormone levels, assist in diagnosing and treating endocrine disorders, and provide a diagnostic basis for adrenal diseases. It holds significant reference value for clinical laboratory testing. Graphical
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