ACS Applied Materials & Interfaces
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2013
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IF 5.008
Tianbin Ren,Wei Wu,Menghong Jia,Haiqing Dong,Yongyong Li,Zhouluo Ou
In the treatment of cancer, multidrug resistance (MDR) has been the major obstacle to the success of chemotherapy. The underlying mechanism relies on the overexpression of drug-efflux transporters that prevent the intracellular transport of the drug. In this study, reduction-cleavable vesicles were designed and developed with efficient glutathione-mediated drug-release behavior for reversing drug resistance. Polymeric vesicles were self-assembled from triblock copolymers with disulfide-bond-linked poly(ethylene glycol) (PEG) and poly(ε-benzyloxycarbonyl-l-lysine) (PzLL). Observations from transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM) outline an obvious hollow structure surrounded by a thin outer layer, indicating the successful formation of the vesicles. Using fluorescently detectable doxorubicin hydrochloride (DOX·HCl) as the model drug, a significant acceleration of drug release regulated by glutathione (GSH) was found (>3-fold difference). Upon incubation of the DOX·HCl-loaded polymeric vesicles with the HeLa cervical cancer cell line exposed to glutathione, an enhanced nuclear accumulation of DOX·HCl was observed, elicited by the preferred disassembly of the vesicle structure under reducing conditions. Importantly, by using the gemcitabine hydrochloride (GC·HCl)-resistant breast cancer cell line MDA-MB-231, it was found that cell viability was significantly reduced after treatment with GC·HCl-loaded polymeric vesicles, indicating that these vesicles can help to reverse the drug resistance.
研究领域:
药理学
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癌症研究
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聚合物化学
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药物递送系统
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DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2021
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IF 8.742
Fang-Fang Chen,Ting Su,Xue-Ting Zhao,Jie-Feng Pan,Li-Fen Liu
Polyamide thin-film composite reverse osmosis (RO) membranes usually suffer from fouling problem, which greatly limits the wide application of RO technology. In this study, a novel rigid-flexible interpenetrating polyamide RO membrane was fabricated via two step modifications to alleviate the membrane fouling during the RO process. The first step modification was carried out by incorporation a new aliphatic diamine of 1,3-diamino-2-propanol (DAPL) into the aromatic m-phenylenediamine (MPD) aqueous solution to react with trimesoyl chloride (TMC) to form a rigid-flexible interpenetration polyamide (PA) separation layer for enhancing its permeability. Then, the second modification was performed by grafting PVA on the top surface of the DAPL-based PA layer to tailor more hydrophilic, smoother and less negatively charged surface and endowed the modified membrane favorable antifouling property. Under the optimized condition, the permeability flux of the modified RO membrane reached 58.6 L/m 2 h with an about 58.8% increase than the pristine RO membrane (about 36.9 L/m 2 h), and the salt rejection was also maintained about 99.6% without any loss. At the same time, the modified RO membrane presented a favorable resistance to the dye foulants of methylene blue (MB) and crystal violet (CV) with flux recovery rate of 76.8% and 84.2% respectively. This study provides a facile and effective way to fabricate a novel antifouling reverse osmosis composite membrane with favorable comprehensive separation performance.
研究领域:
材料科学
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化学工程
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环境工程
✓ 已验证
DOI
高影响因子
POLYMER TESTING
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2020
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IF 3.275
Alberto García-Peñas,Yu Wang,Irene Mena-Palomo,Eduardo López-Collazo,Diana Díaz-García,Santiago Gómez-Ruiz,Florian J. Stadler
Recent advances focused on smart polymers have demonstrated the numerous advantages regarding other structures because they can adapt the behavior depending on physicochemical properties. In this way, functionalized thermoresponsive polymers with organometallic complexes were profoundly analyzed. Consequently, novel catalytic systems or biomedical devices could be developed. This publication focuses on the facile preparation of poly( N -isopropylacrylamide- co -dopamine methacrylamide) copolymers functionalized with triphenyltin chloride by protonolysis through the –OH of catechol groups. The presence of hydrophobic organotin(IV) derivatives could modify the solubility, thermoresponsive behavior, and other properties regarding pure copolymers. Also, sensitive analysis of the microstructure could help to understand the changes associated with the lower critical solution temperature by rheology, UV–vis spectroscopy, and calorimetry. In addition, a preliminary biological study against MDA-MB-231 cancer cells and peripheral blood mononuclear cells showed that the functionalized copolymers could be a potential platform to be explored in the future in the fight against cancer.
研究领域:
聚合物化学
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材料科学
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生物医学工程
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癌症研究
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
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2016
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IF 8.387
Leyong Zeng,Yuanwei Pan,Ruifen Zou,Jinchao Zhang,Ying Tian,Zhaogang Teng,Shouju Wang,Wenzhi Ren,Xueshan Xiao,Jichao Zhang,Lili Zhang,Aiguo Li,Guangming Lu,Aiguo Wu
To avoid the overheating effect of excitation light and improve the efficacy of photodynamic therapy (PDT) of upconversion nanoplatform, a novel nanoprobe based on 808 nm-excited upconversion nanocomposites ( [email protected] @mSiO 2 ) with low heating effect and deep penetration has been successfully constructed for targeted upconversion luminescence , magnetic resonance imaging (MRI) and high-efficacy PDT in HER2-overexpressed breast cancer. In this nanocomposite, photosensitizers (Ce6) were covalently conjugated inside of mesoporous silica to enhance the PDT efficacy by shortening the distance of fluorescence resonance energy transfer and to decrease the cytotoxicity by preventing the undesired leakage of Ce6 . Compared with [email protected] 2 @Ce6, [email protected] @mSiO 2 greatly promoted the singlet oxygen generation and amplified the PDT efficacy under the excitation of 808 nm laser . Importantly, the designed nanoprobe can greatly improve the uptake of HER2-positive cells and tumors by modifying the site-specific peptide, and the in vivo experiments showed excellent MRI and PDT via intravenous injection by modeling MDA-MB-435 tumor-bearing nude mice . Our strategy may provide an effective solution for overcoming the heating effect and improving the PDT efficacy of upconversion nanoprobes, and has potential application in visualized theranostics of HER2-overexpressed breast cancer.
研究领域:
纳米技术
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癌症研究
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治疗诊断学
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生物医学工程
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2022
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IF 3.682
Limin Zhu,Shuangyan Wang
Mesoporous silica nanoparticle (MSN), sodium hyaluronate (SH), silk fibroin (SS), and oxidized sodium carboxymethyl cellulose (O-CMC) hybrids were used to develop an intelligent drug delivery platform that may be employed for pH and redox-responsive bi-drug administration. The first drug, cytarabine (Cyt), was loaded with amino-functionalized mesoporous silica (MSN-NH2) encased by the hydrogel of cystamine (Cys) and SH cross-linked by amide bonds. Hydrophobic doxorubicin (DOX) was co-loaded with Cyt/MSN-NH2/SA in the hydrogel of SS and O-CMC in the Cyt- loaded hydrogel. Dual-responsive drug delivery may be achieved by encapsulating SS and O-CMC in a hydrogel, including Cyt/MSN-NH2/SA/DOX/SS/O-CMC, which has acyl hydrazone bonds (-HC = N) and disulfide bond (-S-S-) exchange reaction with glutathione (GSH). Compared to hydrogels encapsulating only one drug (Cyt or DOX), cell survival analysis revealed that the newly fabricated hydrogels have significantly greater chemotherapeutic efficacy. The cell proliferation of the fabricated nanoparticles was examined in MCF-7 and MDA-MB-231 cells, which indicates that the nanoparticles effectively kill the cancer cells without affecting non-cancerous cells. Further, we effectively investigated the morphological changes, and various biochemical staining methods examined nuclear fragmentation/condensation. Furthermore, the biosafety of the nanoparticles was investigated by the in vivo animal model, which reveals that they remarkably enhanced the safety profile in various organs. These outcomes demonstrated that this nanoparticle platform was a promising beneficial agent for improving breast cancer treatment.
研究领域:
纳米技术
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药学
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癌症研究
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材料科学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2023
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IF 9.5
Shan Xiao,Lu Guo,Chen Ai,Mengmeng Shang,Dandan Shi,Dong Meng,Xiao Sun,Xiaoxuan Wang,Rui Liu,Yading Zhao,Jie Li
Multiple drug resistance (MDR) exists in divergent cancers including triple negative breast cancer (TNBC) and partly results in the resistance to many first-line anti-cancer agents, bringing a big challenge to TNBC management. To develop novel TNBC therapeutics, in our study, a hyaluronic acid (HA)–carboxymethyl chitosan (CMC) conjugate linked via a disulfide-bond (HA-SS-CMC, HSC) was synthesized to fabricate nanodroplets (NDs). The NDs encapsulating doxorubicin (DOX) and perfluorohexane (DOX-HSC-NDs) were prepared via a homogenization/emulsification strategy and exhibited not only high biocompatibility but also noticeable tumor cell targeting ability and dual pH/redox responsiveness. Besides, DOX-HSC-NDs can be used as a contrast-enhanced ultrasound imaging agent for specific tumor imaging. DOX-HSC-NDs in combination with ultrasound targeted microbubble destruction could improve intracellular drug aggregation and retention of MDR cells and work against multiple mechanisms of drug resistance through synergistic strategies, including up-regulating the reactive oxygen species (ROS) level, promoting apoptosis, and scavenging glutathione, while reducing the expression levels of P-glycoprotein and inhibiting the epithelial–mesenchymal transition. This combination strategy showed protective effects against TNBC in both MDA-MB-231/ADR cells and tumor-bearing mice. Our study for the first time developed and reported the ultrasound-augmented HSC-NDs as the DOX nanocarrier and provided scientific evidence to support the future application of DOX-HSC-NDs as a potential TNBC therapy.
研究领域:
癌症治疗
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纳米技术
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药学
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生物医学工程
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肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Airong Xu,Yiming Gong,Qingqing Sun,Liuzemu Li,Fen Wang,Zhihong Xiao,Rukuan Liu
GO/HEC/PGDE/Fe 3 O 4 materials were successfully fabricated using environmentally-friendly hydroxyethyl cellulose (HEC), poly(ethylene glycol) diglycidyl ether (PGDE), graphene oxide (GO) and magnetic Fe 3 O 4 . Systematic investigations were completed to explore the influences of GO content in GO/HEC/PGDE/Fe 3 O 4 and adsorption conditions on the adsorptions of cationic dyes (methylene blue (MB), crystal violet (CV)) and anionic dye acid blue 25 (AB-25). The increase of GO content can remarkably improve the adsorption capacity of GO/HEC/PGDE/Fe 3 O 4 for the dyes. The three kinetic, four isothermic and three thermodynamic models were investigated to reveal the adsorption behaviors of the dyes. The formation of HEC/PGDE/Fe 3 O 4 and adsorption mechanisms of the dyes by GO/HEC/PGDE/Fe 3 O 4 were suggested. The GO/HEC/PGDE/Fe 3 O 4 endowed with easy-fabrication, eco-friendly feature, efficient adsorption capacity of anionic/cationic dyes, convenient separation and reusability has potential applications in wastewater purification industry.
研究领域:
环境科学
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材料科学
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化学工程
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水处理
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产品关联
DOI
PubMed
高影响因子
Journal of Water Process Engineering
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2024
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IF 6.3
Mei Dong,Kuilong Liu,Guoqiang Song,Guiping Ma
In this study, MOF-on-MOF hybrids were successfully synthesized by interfacial growth method using CoZn-ZIF as guest MOF and MIL-101(Fe) as host MOF, and then employed as heterogeneous catalysts to activate peroxymonosulfate (PMS) in the degradation of dyes. The hybrids exhibited different morphologies depending on the Co/Zn ratios (10/0, 10/2, 10/10, and 2/10), all of which displayed exceptional catalytic performance. Notably, the hybrid with a Co/Zn ratio of 10/2 showed the highest degradation rate of MB, reaching 100 % removal within 3 min with a k obs value of 452.75 Lg −1 min −1 based on the pseudo-second-order kinetic model. Through EPR characterization and scavenging experiments, it was determined that the degradation of dyes occurred through a combination of radical (SO 4 •-, •OH)and non-radical ( 1 O 2 ) pathways, with 1 O 2 and SO 4 •- playing prominent roles. Various factors such as Co/Zn ratio, catalyst dosage, PMS concentration, initial pH, temperature, and MB solution concentration were systematically investigated to understand their influence on the catalytic performance. Furthermore, the influence of inorganic ions, humic acid (HA), and the reusability and stability of the catalyst was evaluated, demonstrating its potential for practical applications. Overall, this research provides valuable insights into the design and application of MOF-based catalysts for environmental remediation processes.
研究领域:
环境科学
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催化
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材料科学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
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2020
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IF 8.758
Shaoping Yin,Yi Gao,Yu Zhang,Jianan Xu,Jianping Zhu,Fang Zhou,Xiaochen Gu,Guangji Wang,Juan Li
Deep tumor penetration, long blood circulation, rapid drug release, and sufficient stability are the most concerning dilemmas of nano-drug-delivery systems for efficient chemotherapy. Herein, we develop reduction/oxidation-responsive hierarchical nanoparticles co-encapsulating paclitaxel (PTX) and pH-stimulated hyaluronidase (pSH) to surmount the sequential biological barriers for precise cancer therapy. Poly(ethylene glycol) diamine (PEG-dia) is applied to collaboratively cross-link the shell of nanoparticles self-assembled by a hyaluronic acid–stearic acid conjugate linked via a disulfide bond (HA–SS–SA, HSS) to fabricate the hierarchical nanoparticles (PHSS). The PTX and pSH coloaded hierarchical nanoparticles (PTX/pSH-PHSS) enhance the stability in normal physiological conditions and accelerate drug release at tumorous pH, and highly reductive or oxidative environments. Functionalized with PEG and HA, the hierarchical nanoparticles preferentially prolong the circulation time, accumulate at the tumor site, and enter MDA-MB-231 cells via CD44-mediated endocytosis. Within the acidic tumor micro-environment, pSH would be partially reactivated to decompose the dense tumor extracellular matrix for deep tumor penetration. Interestingly, PTX/pSH-PHSS could be degraded apace by the completely activated pSH within endo/lysosomes and the intracellular redox micro-environment to facilitate drug release to produce the highest tumor inhibition (93.71%) in breast cancer models.
研究领域:
纳米技术
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癌症治疗
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药物递送系统
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Junjie Wang,Chunxiang Xu,Milan Lei,Yi Ma,Xiaoxuan Wang,Ru Wang,Jianli Sun,Ru Wang
The detection of cardiac markers with high sensitivity and specificity is exceedingly essential for the early diagnosis and warning of cardiovascular disease. In this work, we developed a microcavity-based sandwich immune chip to realize the ultrasensitive detection of cardiac troponin I (cTnI) and creatine kinase isoenzyme MB (CK-MB) by surface-enhanced Raman spectroscopy (SERS) method. Herein, polystyrene microspheres modified with gold nanoparticles (Au NPs) were fixed on the substrate via polydopamine, which was used as an immune capture chip. 5,5′-Dithio bis-(2-nitrobenzoic acid) (DTNB) and 4-mercaptobenzoic acid (4MBA) labelled Au NPs served as immune signal probes for cTnI and CK-MB, respectively. Light confinement effect of polystyrene (PS) microcavities and localized surface plasmon resonance (LSPR) near the Au NPs synergistically strengthened the electromagnetic field and promoted the interaction between light and matter, which enhanced SERS performance with an enhancement factor of 1.95 × 10 12 for SERS sensing. The immunoassay results demonstrated the sensitive quantitative detection with the limit of detection (LOD) of 3.16 pg/mL and 4.27 pg/mL for cTnI and CK-MB, separately. The method also had good specificity, reproducibility and stability. It could be expected that our proposed microcavity-based SERS chip immunoassay technique would be considered as an effective diagnostic tool for ultrasensitive detection of cardiac biomarkers.
研究领域:
心脏病学
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纳米技术
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分析化学
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生物医学工程
✓ 已验证
DOI
高影响因子
APPLIED CATALYSIS A-GENERAL
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2022
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IF 5.723
Chencheng Dong,Yan Bao,Mingyang Xing,Masakazu Anpo,Jinlong Zhang
Advanced oxidation processes (AOPs) are reckoned effective for removing persistent and recalcitrant pollutants by reactive oxygen species (ROSs), including hydroxyl radicals (•OH), sulfate radicals (SO 4 • - ), and superoxide radicals (•O 2 - ), as well as singlet oxygen ( 1 O 2 ). In this work, the Mo 2 C quantum dots enriched N-doped graphitic carbon layers (Mo 2 C QDs/NGCLs) were constructed via a facile two-step methodology. Interestingly, the Mo 2 C QDs/NGCLs acted as a highly efficient cocatalyst and/or catalyst simultaneously in AOPs, in particular visible-light-driven (VLD) Fenton and VLD PMS activation. In the hydroxyl radical-based AOPs (•OH-AOPs), it exhibited a remarkable removal efficiency for a range of aromatic organic pollutants , including rhodamine B (RhB) (99.9 %), phenol (90 %), benzophenone-3 (BZP) (83 %), methyl orange (MO) (58 %), methyl blue (MB) (89 %), ibuprofen (IBU) (100 %), and carbamazepine (CBZ) (50 %). Sulfate radicals-based AOPs (SR-AOPs) effectively degrade RhB (72 %), MO (83 %), IBU (70 %), and BZP (100 %). Thus, the present findings confirmed the potential of transition metal carbides for AOP applications and established a solid theoretical and technical basis for further research.
研究领域:
环境科学
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材料科学
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化学工程
·
水处理
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）