查找使用阿拉丁产品的科研文献

MIL-68(Fe) as an efficient visible-light-driven photocatalyst for the treatment of a simulated waste-water contain Cr(VI) and Malachite Green

Fenfen Jing,Ruowen Liang,Jinhua Xiong,Rui Chen,Shiying Zhang,Yanhua Li,Ling Wu

A highly effective Iron Metal-Framework photocatalyst (MIL-68(Fe)) has been successfully prepared via a facile solvothermal method under acidic condition. The UV–vis diffuse reflectance spectrum reveals that the absorption edge of MIL-68(Fe) is 440   nm. The flat-band potential of MIL-68(Fe) is −0.6   V vs. NHE at pH   =   6.8, which is more negative than the redox potential of Cr(VI)/Cr(III) (+0.51   V, pH   =   6.8). Consequently, it is thermodynamically permissible for the transformation of photogenerated electrons to the Cr(VI) to produce Cr(III). Moreover, MIL-68(Fe) could perform as an efficient photocatalyst towards the reduction of Cr(VI) aqueous with a wide pH range. After 5   min of visible light illumination (λ   >   420   nm), almost 100% Cr(VI) can convert to Cr(III) with (NH 4 ) 2 C 2 O 4 as a scavenger (pH   =   3), which is also higher than that of N-doped TiO 2 (50%) and ZnO (7.6%) under identical conditions. Furthermore, MIL-68(Fe) is proved to perform as a highly efficient photocatalyst for remove of different aqueous contaminant with malachite green (MG) as a scavenger. Finally, a possible reaction mechanism has also been investigated in detail.

Generation of functional single-chain fragment variable from hybridoma and development of chemiluminescence enzyme immunoassay for determination of total malachite green in tilapia fish

Jiexian Dong,Zhenfeng Li,Yu Wang,Maojun Jin,Yudong Shen,Zhenlin Xu,A.M. Abd El-Aty,Shirley J. Gee,Bruce D. Hammock,Yuanming Sun,Hong Wang

To determine malachite green (MG) and its major metabolite, leucomalachite green (LMG) residual levels in tilapia fish, chemiluminescent enzyme immunoassay (CLEIA) was developed based on a single-chain variable fragment (scFv)-alkaline phosphatase (AP) fusion protein. At first, V H and V L gene sequences were cloned from hybridoma cell lines secreting monoclonal antibody against LMG, and then thoroughly by database-assisted sequence analysis. Finally, the productive V H and V L were assembled to an intact scFv sequence and engineered to produce scFv-AP fusion protein. The fusion protein was further identified as a bifunctional reagent for immunoassay, then a sensitive one-step CLEIA against LMG was developed with a half-maximal inhibitory concentration (IC 50 ) and limit of detection (LOD) of 1.3 and 0.04 ng/mL, respectively. The validation results of this novel competitive CLEIA was in line with those obtained by classical HPLC method for determination of total MG in spiked and field incurred samples.

Rapid detection of malachite green residues in fish using a surface-enhanced Raman scattering-active glass fiber paper prepared by in situ reduction method

Di Deng,Qiuyuan Lin,Hua Li,Zhipeng Huang,Yunyan Kuang,Hui Chen,Jilie Kong

Food safety issues, especially those related to drug residues , have caused concern in recent years. Malachite green (MG), which is frequently used as ectoparasiticide, fungicide , and antiseptic in fish farming, is poisonous and carcinogenic . Several technologies and methods have been developed to accurately detect MG, but they can be time-consuming and expensive. Herein, we prepared a glass fiber paper as a surface-enhanced Raman scattering substrate for rapid and convenient detection of MG residues in fish. Uniform silver nanoparticles were deposited on the glass fiber paper by two in situ reduction steps of silver nitrate. The Raman signal of MG was detected and recorded using a portable Raman spectrometer in 10 s. There was a good linear relationship between the surface-enhanced Raman scattering signal intensity and the concentration of MG between 1 × 10 −7 and 1 × 10 −5 mol/L and the limit of detection was 5 × 10 −10 mol/L. Residual MG in fish samples was extracted and detected with a recovery rate range of 98.9–119.4%. This method provides a novel way to detect MG in fish.

A novel dual-excitation ratiometric fluorescence sensor based on nitrogen-doped carbon dots for simultaneous detection and discrimination of chloramphenicol and malachite green in fish

Wendi Xiao,Yunkai Zhang,Siting Peng,Junyuan Shi,Wen Wang,Zida Wang,Ting Zhang,Da Chen

First simultaneous detection and discrimination of CAP and MG. • Ratiometric detection of CAP and MG using dual excitation N-CDs. • CAP and MG exhibit distinct quenching mechanisms (IFE vs IFE/FRET). • A handheld smartphone platform with N-CDs/PVA film enables visual detection. • Satisfactory recovery is achieved in aquatic product samples. The expanding aquaculture industry, driven by growing demand for aquatic products, has raised serious concerns about residual chloramphenicol (CAP) and malachite green (MG) due to their detrimental impact on human health and the environment. Herein, a dual-excitation ratiometric fluorescence probe based on nitrogen-doped carbon dots (N-CDs) is developed for simultaneously detecting and discriminating CAP and MG. Employing a hydrothermal approach with 5-aminoisophthalic acid and biuret as precursors, the synthesized N-CDs possess two excitation peaks (286 nm and 348 nm) and corresponding emission maximum at 404 nm. The presence of CAP significantly quenches the 286 nm excitation peak of N-CDs via the internal filtering effect (IFE), while leaving the 348 nm peak largely unaffected. This selective quenching yields a linear range of 0.045–100 μM with a limit of detection (LOD) as low as 45 nM. In contrast, the fluorescence quenching by MG is mediated principally by fluorescence resonance energy transfer and IFE, leading to a pronounced quenching efficacy at two excitation wavelengths, thus achieving a low LOD (73 nM) within 0.073–125 μM. Furthermore, the ability to differentiate between CAP and MG in mixed samples is achieved through linear discriminant analysis and hierarchical cluster analysis, leveraging the differential quenching responses of N-CDs. Additionally, a smartphone-integrated sensing platform is constructed for the detection of CAP and MG, achieving satisfactory recovery rates in aquatic environments. In summary, this research establishes a dual-analyte ratiometric sensing approach for CAP and MG, offering a practical tool for food safety and environmental surveillance. Download: Download high-res image (186KB) Download: Download full-size image

Magnetic nanoporous carbon-based self-enriched and self-cleaning recyclable substrate Co/C@Ag/g-C3N4 for SERS detection and photocatalytic degradation of malachite green

Guotong Chen,Fang Mi,Shan Zhang,Xuehui Rao,Jian Sun,Zhong Yang,Ming Guan

This study reports the design and fabrication of a magnetic nanoporous carbon-based recyclable surface-enhanced Raman scattering (SERS) substrate Co/C@Ag/g-C 3 N 4 , which can simultaneously achieve SERS detection and visible-light photocatalytic degradation of malachite green (MG). Co/C and g-C 3 N 4 can spontaneously adsorb and concentrate MG near plasmonic nanoparticles, thereby enhancing the SERS response. The substrate exhibited a SERS enhancement factor of approximately 1.3 × 10 6 . The limit of detection (LOD) for MG in aquaculture water, prawn, and tilapia was 8.2 × 10 −11  M, 0.08 μg/kg, and 0.06 μg/kg, respectively. The substrate can be magnetically collected from the complex matrix and regenerated through photocatalytic degradation. Eliminates the co-centrifugation of the SERS substrate with the complex matrix. Even after 4 regeneration cycles, the substrate retained nearly 80 % of its original SERS intensity. This study presents a novel strategy for constructing self-enriched, self-cleaning, and recyclable substrates with promising applications in food safety and environmental protection.

A Novel Solid-State Electrochemiluminescence Sensor Based on poly(3-amino-4 hydroxybenzenesulfonic acid) /Ru(bpy)3 2+ Modified Electrode for Determination of Malachite Green

Qiu-feng Lan,Wen-chang Wang,Xiao-hui Chen,Zhi-dong Chen

The poly(3-amino-4-hydroxybenzenesulfonic acid)/Ru(bpy) 3 2+ modified glassy carbon electrode (PAHBS/Ru(bpy) 3 2+ /GCE) is a novel solid-state electrochemiluminescence (ECL) sensor. It was prepared by electropolymerization of 3-amino-4-hydroxybenzenesulfonicacid (AHBS) followed by soaking into Ru(bpy) 3 2+ solution. Ru(bpy) 3 2+ was entrapped on the electrode via the electrostatic interactions between sulfonic acid groups and Ru(bpy) 3 2+ cation. The morphology and composition of the modified electrodes were analysed using scanning electron microscope and energy-dispersed spectrum. Cyclic voltammetry and electrochemical impedance spectroscopy were used to compare the electrochemical behavior of the electrodes before and after modification. Under the optimal conditions, the ECL intensity of the quenching value (ΔECL) versus the logarithm of the concentration of malachite green (MG) was linear over a concentration range from 5.0 × 10 -8 to 1.0 × 10 -5 mol L -1 ( r = 0.9931) and the limit of detection (LOD) was 2.5 × 10 -8 mol L -1 . This method has been successfully applied to the determination of MG in pond water.

Fabrication of paper-based SERS substrates by spraying silver and gold nanoparticles for SERS determination of malachite green, methylene blue, and crystal violet in fish

Yang Guohai,Fang Xuejiao,Jia Qin,Gu Haixin,Li Yunpeng,Han Caiqin,Qu Lu-Lu

A disposable surface-enhanced Raman scattering (SERS) substrate was prepared by successively spraying silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) onto commercial filter paper using an inexpensive consumer sprayer. The strong surface enhancement of AgNPs and chemical stability of AuNPs can be advantageously combined. The substrate exhibited excellent SERS activity for malachite green (MG), methylene blue (MB), and crystal violet (CV) under 785-nm excitation, with limits of detection (LODs) of 4.3 × 10 −9 , 2.0 × 10 −8 , and 8.1 × 10 −8  M, respectively. The substrate exhibited long-term stability, and it can be stored under ambient conditions for 4 weeks with a relative standard deviation of less than 3% among peak intensities. The substrate also showed good reproducibility with a relative standard deviation of 7.1% among different substrate peak intensities. The substrates enable on-site determination of residual fishery drugs and distinguish MG, MB, and CV mixtures in spiked fish within 5 min, and the average recoveries in fish scales and fish meat were better 90.1% and 76.9%, respectively. The method exhibited rapidity, simplicity, and high sensitivity and is expected to be used for the screening of additives in food.

Cobalt-doped nanoporous carbon as SALDI-TOF-MS adsorbent and matrix for quantification of cetyltrimethylammonium bromide, Rhodamine B and Malachite Green at sub-ppt levels

Zhen Deshuai,Jiang Ning,Geng Hongchao,Qiao Yan,Liu Yu,Zhu Xingqi,Gao Chan,Grimes Craig A.,Cai Qingyun

Cobalt-doped nanoporous carbon (Co-NPC) with dodecahedral shape was pyrolytically synthesized and applied as a sorbent and matrix for the enrichment and analysis of small molecules by surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF MS). Extremely low detection limits were accomplished for cetyltrimethylammonium bromide (1 fg·mL −1 ), and Rhodamine B (1 fg·mL −1 ) in water, and Malachite Green and its metabolite in fish blood and fish extracts (pg·mL −1 concentrations). Schematic representation of cobalt-doped nanoporous carbons (Co-NPCs) applied as SALDI matrix for analysis of toxic contaminants in fish and receipt papers. The Co-NPCs have a high desorption/ionization efficiency and low limit of detection.

Magnetic solid-phase extraction based on Fe3O4/graphene oxide nanoparticles for the determination of malachite green and crystal violet in environmental water samples by HPLC

Leilei Zhang,Yiying Zhang,Yurong Tang,Xiaorong Li,Xinfeng Zhang,Chaobi Li,Shuxia Xu

Rapid and sensitive detection of trace malachite green and its metabolite in aquatic products using molecularly imprinted polymer-coated wooden-tip electrospray ionization mass spectrometry

Yanying Huang,Yanfang Ma,Huawen Hu,Pengran Guo,Lei Miao,Yunyun Yang,Min Zhang

In this study, a molecularly imprinted polymer-coated wooden-tip (MIPCWT) electrospray ionization mass spectrometry (ESI-MS) method was developed for rapid and sensitive detection of trace malachite green (MG) and its metabolite in aquatic products. Such a method was realized by applying a silicone-modified acrylate molecularly imprinted emulsion (SMAMIE) onto the surface of wooden tips to specially design a MIPCWT solid-phase micro-extraction (SPME) probe for selective enrichment of MG and its metabolite from aquatic products. Subsequently, a high voltage and some spray solvent were applied to the MIPCWT SPME probe, and ESI was induced for direct MS analysis under ambient and open-air conditions. The MIPCWT-SPME probe exhibits a high enriching capacity of approximately 1500–2000 fold toward MG and leucomalachite green (LMG), with detection limit reaching 0.01 μg L−1. In addition, a good linearity is obtained for both MG and LMG, with correlation coefficient values (R2) of no less than 0.998. The present method was successfully applied to analyze MG and LMG in real-life tap water, river water and fish samples, and good recoveries in the range of 93–103%, 92–108% and 106–113%, respectively, were found. All of these demonstrated that our developed MIPCWT-ESI-MS method holds great potential for rapid, direct, sensitive, and reliable detection and analysis of trace veterinary drug residues in aquatic products.

P(AAS-co-AMPS-Na)/SA/laponite composite hydrogel beads with excellent performance prepared by photopolymerization under green LED irradiation for adsorption of malachite green

Jiahui Li,Boxuan Peng,Siyuan Yu,Fangzheng Zhao,Qiqi Han,Shuaibing Huang,Fengmin Jin,Jinfeng Xing

With the rapid development of industry, water pollution has become one of the important problems that harm the ecological environment and human health. The extensive use and random discharge of dyes have caused serious damage to water resources. Hydrogel bead is a promising material for dye adsorption due to its advantages of rich functional groups and high specific surface area. Here, acrylic acid sodium salt (AAS) and 2-acrylamide-2-methylpropanesulfonic acid (AMPS) as monomers, N,N’-Methylene-bis-acrylamide (MBA) as crosslinking agent, sodium alginate (SA) as semi-interpenetrating network component, laponite as nano-clay additive, and single 2′,4′,5′,7′ −tetrabromofluorescein disodium salt (EY)/ Triethanolamine (TEOA)/ diphenyl iodonium hexafluorophosphate (DPI) as green light polymerization initiating system were combined to prepare composite hydrogel beads within 10 s under green LED irradiation. The composite hydrogels were characterized by infrared spectroscopy (FTIR), thermogravimetric analyzer (TG), scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The addition of sodium alginate and laponite can improve the mechanical properties of hydrogel beads. In the adsorption study of malachite green, the influence of time, temperature, dye concentration, pH, ionic strength, and other factors on the adsorption effect were investigated. The adsorption process was consistent with the second-order kinetic process and Langmuir adsorption. At room temperature, the highest adsorption capacity of the hydrogel beads for malachite green reached 829.63 mg/g. The PAAS-SL hydrogel beads maintained a high adsorption effect in the pH range (3–10), and the capacity reached 575.0 mg /g at pH 3, which had a good ability to resist low pH. In the adsorption–desorption experiments, the removal rate of PAAS-SL hydrogel beads for MG is still more than 80 % after 5 cycles. Our research provides a promising strategy for the efficient and rapid preparation of hydrogel beads with excellent properties.

Surface-enhanced Raman spectroscopy with partial least squares regression for rapid and accurate detection of malachite green in aquaculture water using large-size gold nanoparticles

Xinhui Zhou,Zhen Li,Yinfeng Hao,Qingling Duan,Cong Wang,Tan Wang,Daoliang Li

Malachite green is not used in aquaculture owing to its severe toxic effects on humans and other living organisms. The presence of this compound in fishery water should be quantified and reported in all water quality reports. In this paper, a method based on surface-enhanced Raman scattering combined with partial least squares regression for rapid, accurate, reliable, and sensitive detection of trace malachite green in fishery water is described. A prepared sol of 100-nm-size gold particles was used as an active substrate for Raman spectral data acquisition. Three parameters that affect detection, namely, the volume of gold nanoparticles (400 µL), the volume of sodium chloride (300 µL), and the adsorption time (8 min) were optimized in single-factor experiments. Under the optimal condition, the proposed method demonstrated a wide linear range of detectable concentrations (0.01–5.0 µmol/L) and the limit of detection was 2.7 × 10−9 M. The proposed method successfully detected spiked malachite green in fishery water samples, with satisfactory recoveries and relative standard deviations. Thus, this study suggests a rapid, simple, accurate, and sensitive approach for detecting malachite green residues in fishery water samples, which is promising for wide applications in environmental monitoring and food safety.