查找使用阿拉丁产品的科研文献

Bioactive ZIF-8@PEO/PLA/PCL electrospun nanofibrous membrane for enhancing the preservation stability of blueberry

Junying Wang,Chong Shi,Donglu Fang,Yankang Deng,Tao Lu,Shuqiong Xia,Mengyang Xu,Lianfei Lyu,Yaqiong Wu,Chaobo Huang,Fuliang Cao,Weilin Li

For enhancing the storability and quality of postharvest fresh products, an electrospun nanofiber membrane composed of polylactic acid (PLA), polycaprolactone (PCL), perilla essential oil (PEO) and zeolitic imidazolate framework (ZIF-8) (ZIF-8@PEO/PLA/PCL) was developed for active food packaging. The ZIF-8@PEO/PLA/PCL electrospun fibers integration were continuous nanofibers with ZIF-8 nanoparticles affixed to their surface and 759.1 nm average diameter. The ZIF-8@PEO/PLA/PCL nanofiber membranes had improved tensile strength of 14.35 MPa and anti-deformation capability of 9.33 MPa and reduced thermal stability compared with PEO/PLA/PCL. The adhesion of ZIF-8 nanoparticles maintained the nanofiber surface hydrophobicity and improved the water vapor barrier quality of 0.21 g mm/m 2 h. The ZIF-8@PEO/PLA/PCL nanofiber membrane showed excellent antibacterial ability within 24 h test and biosafety confirmed by the zebrafish co-culture experiments. The storage test revealed that the ZIF-8@PEO/PLA/PCL membrane delayed the postharvest quality degradation of blueberry fruits to extend their shelf-life to 28 days by maintaining firmness, color, and nutritional quality. These results indicated that the nanofiber membrane has significant potential for fruit preservation as functional packaging material.

Evaluation of Remodeling and Regeneration of Electrospun PLGA@PCL/Elastin Small-Diameter Vascular Grafts In Vivo

Yonghao Xiao,Yuanguo Zhang,Yuehao Xing,Ming Gao,Yuting Ge,Hailei Li,Xin Wu,Lin Ye,Zeng-guo Feng

Bypass vascular grafting is an effective treatment for cardiovascular disease. Currently, small-diameter vascular grafts (SDVGs, inner diameter <6 mm) for peripheral revascularization, especially for lower limb revascularization, are in high demand. Previously, we prepared and investigated PLGA@PCL SDVGs with core/shell fibers. In this study, PLGA@PCL/elastin core/shell fibers (PCEs) SDVGs were further prepared by electrospinning, so as to enhance the vascular regeneration performance through the introduction of exogenous soluble elastin. The cross-linking effects of elastin with different cross-linking reagents on PCEs SDVGs were subsequently explored. Among them, PCEs SDVGs cross-linked with 1-ethyl-3-(3-(dimethylamino)propyl) carbodiimide (EDCI) and N-hydroxysuccinimide (NHS) retained the most soluble elastin content and achieved a compliance of 1.74 ± 0.62%/mmHg × 10–2, a bursting pressure of 1750 mmHg, and a tensile strength of 2.09 ± 0.51 MPa, which are mostly close to those of human saphenous vein. Subsequently, in vivo transplantation in the abdominal aorta showed that all implanted SDVGs cross-linked by EDC/NHS (PCEsEN) remained patent without aneurysm formation. Histological staining results further showed that the introduction of elastin not only promoted the degradation of SDVGs but also accelerated the formation of neointimal tissue and suppressed the calcification. Thus, PCEsEN shows promising potential to be translated into SDVGs in clinic.

PCL/sulfonated keratin mats for vascular tissue engineering scaffold with potential of catalytic nitric oxide generation

Jie Dou,Yanfang Wang,Xingxing Jin,Pengfei Li,Lijuan Wang,Jiang Yuan,Jian Shen

Heparin-like polymers have a good anticoagulant effect due to some groups similar to heparin. Herein, sulfonated keratin(SK) was prepared by chain transfer radical polymerization of 3-sulfopropyl methacrylate(SPMA) to improve blood coagulation nature of keratin. Poly(ε-caprolactone)(PCL)/SK nanofibrous mats with the ratio of 7 and 3 were then fabricated by electrospinning. In vitro cytotoxicity and blood compatibility tests were performed to assess the biocompatibility. Viability of NIH 3T3 cells on PCL/SK mats was higher than that on the pristine PCL mats, indicating their good cytocompatibility. These sulfonated keratin-containing mats enhanced endothelial cell growth, while inhibited smooth muscle cell proliferation and reduced platelet adhesion in the presence of GSH and GSNO, as a result of NO generation. Furthermore, the biocomposite mats prolonged the activated partial thromboplastin time(APTT) effectively without hemolysis. Taken together, PCL/SK mats are potential for applications in vascular tissue engineering.

Surface-Localized Crosslinked MEW PCL–Hydrogel Scaffolds with Tunable Porosity for Enhanced Cell Adhesion and Viability

Yixin Li,Le Kang,Kai Cao

Hydrogel is widely used as a scaffolding material for tissue engineering due to its excellent cytocompatibility and potential for biofunctionalization. However, its poor mechanical property limits its further application. Fabrication of fiber-reinforced hydrogel composite scaffolds has emerged as a solution to overcome this problem. However, existing strategies usually produce nonporous composite scaffolds, where the interfiber pores are completely filled with hydrogel. This design can hinder oxygen and nutrient exchange between seeded cells and the culture medium, thereby limiting cell invasion and colonization within the scaffold. In this study, sodium alginate (SA) hydrogel was exclusively grafted onto the surface of the constituent fibers of the melt electrowritten scaffold while preserving the porous structure. The grafted hydrogel amount and pore size were precisely controlled by adjusting the SA concentration and the crosslinking ratio (SA: CaCl2). Experimental results demonstrated that the porous composite scaffolds exhibited superior swelling capacity, degradation ratio, mechanical properties, and biocompatibility. Notably, at an SA concentration of 0.5% and a crosslinking ratio of 2:1, the porous composite scaffold achieved optimal cell adhesion and viability. This study highlights the critical importance of preserving porous structures in composite scaffolds for tissue-engineering applications.

SHED-exosomes functionalized micro-nano bioactive glass/PCL membrane regulates macrophage polarization and promotes osteogenic differentiation

Yan Yongyong,Lan Haifeng,Miao Guohou,Wang Haiyan,Li Zhengmao,Zhang Qing,Wang Shuai,Wu Gang,Jaspers Richard T.,Pathak Janak L.

Micro-nano bioactive glass (MNBG) has bone regenerative potential. However, the difficulty of MNBG molding restricts its clinical applications in bone regeneration. Exosomes carry proteins, lipids, and nucleic acids for communication between cells. In this study, we adhered human exfoliated deciduous teeth (SHED)-derived exosomes (SHED-Exos) to electrospun micro-nano bioactive glass/polycaprolactone (MNBG/PCL) membrane to control inflammation and promote bone regeneration. MNBG/PCL membrane showed biocompatibility and osteogenic ability. Next, we demonstrated that SHED-Exos internalized into macrophages attenuated LPS-induced expression of M1-macrophage markers iNOS, IL-6, and IL-1β and upregulated M2-macrophage marker IL-10 expression, indicating a switch from M1 to M2 macrophage phenotype. MNBG/PCL-loaded SHED-Exos membrane supported the survival and growth of mouse bone marrow stromal cells (mBMSCs). When M1 macrophages were co-cultured with mBMSCs on the membrane, the SHED-Exos-loaded membrane showed higher Runx2, Alp, and Ocn gene expression. Our findings indicate that SHED-Exos-functionalized MNBG/PCL membrane has anti-inflammatory and osteoinductive potential, suggesting its potential as a candidate material for bone regeneration.

Confined crystallization and degradation of six-arm star PCL with core of cyclotriphosphazene in epoxy thermosets

Pengli Zhang,Yanfang Zhao,Rentong Yu,Jianhe Liao

Six-arm star polycaprolactone with core of cyclotriphosphazene ( s -PCL) was synthesized via ring-opening polymerization and characterized by NMR and GPC . Epoxy thermosets incorporating 10–40  wt % of this copolymer were cured with 4,4′-methylenebis(2-chloroaniline) (MOCA) and 4,4′-diaminodiphenyl sulfone (DDS), respectively. In these thermosets, intermolecular specific interactions had a significant impact on their morphologies. Specifically, no discernable macroscopic phase separation was found in FESEM images of MOCA-cured epoxy resins . However, for the DDS-cured system, s -PCL was partially miscible with the epoxy thermosets, as evidenced with FTIR and DMA. This is may be owning to enriched concentration of hydroxyl groups in the s -PCL favored the miscibility between PCL subchains of carbonyl and the epoxy matrix. Dispersed phase, further confirmed by XRD to be amorphous , with a diameter of 0.34–0.44 μm could be observed ( s -PCL’ content ≤ 20  wt %) in this system. Nevertheless, crystallization of PCL could not be suppressed completely ( s -PCL’ content > 30  wt %). Besides, epoxy thermosets containing crystalline PCL were found to be more resistant to thermal degradation but sensitive to alkaline degradation (10% NaOH aqueous solution) according to TGA and FESEM results. This work presented here is a meaningful report on the presence of alkaline-labile crosslinking points through the whole networks.

Heparinized PCL/keratin mats for vascular tissue engineering scaffold with potential of catalytic nitric oxide generation

Xiuzhen Wan,Yanfang Wang,Xingxing Jin,Pengfei Li,Jiang Yuan,Jian Shen

Design of poly(mPEGMA-co-MAA) hydrogel-based mPEG-b-PCL nanoparticles for oral meloxicam delivery

Yongli Shi,Zhaomin Liu,Yaxing Yang,Xiaojie Xu,Yan Li,Tong Li

To enhance the therapeutic effects of meloxicam (MLX), we developed an oral MLX-loaded poly(ethylene glycol)-b-poly(ε-caprolactone) nanoparticles@hydrogel (MLX-NPs@hydrogel) preparation. The MLX-NPs were fabricated via a solvent evaporation method, and their morphologies were observed by a JEM-1011 transmission electron microscope (TEM). The poly(mPEGMA- co -MAA) hydrogels were synthesized, and studies on their pH sensibilities were carried out in pH   1.2, 6.8, and 7.4 buffers. The final MLX-NPs@hydrogel preparation was obtained by immersing the hydrogels in the MLX-NPs suspensions (pH   7.4) for 48   h. The thermodynamic properties and cytotoxicity of the MLX-NPs@hydrogel preparation were also studied. TEM images illustrated that mPEG-b-PCL NPs had a uniform size distribution. The poly(mPEGMA- co -MAA) hydrogels showed an excellent pH-sensibility. Thermal gravity analysis (TGA) data suggested that the protection of hydrogels improved the stability of mPEG-b-PCL NPs. The release studies revealed that MLX-NPs@hydrogel could deliver the MLX-NPs into alkalescent environment ( e.g. intestinal tract). Then, the medicated NPs released MLX at a sustained release profile. Such preparation could overcome the drawbacks of oral MLX, and enhance its therapeutic effects. Therefore, the NPs@hydrogel was a promising sustained-controlled release matrix.

Chitosan/PCL nanofibrous films developed by SBS to encapsulate thymol/HPβCD inclusion complexes for fruit packaging

Chaoyi Shen,Menglu Wu,Cui Sun,Jiangkuo Li,Di Wu,Chongde Sun,Yong He,Kunsong Chen

In this work, the solution blow spinning (SBS) technique was used to rapidly fabricate the thymol (THY)/2-hydroxypropyl-β-cyclodextrin (HPβCD) inclusion complexes loaded chitosan (CS)/polycaprolactone (PCL) nanofibrous films for fruit preservation and packaging. XRD results indicated that the THY/HPβCD inclusion complexes were successfully incorporated into the CS/PCL nanofibers . The nanofibrous films had an increase of average diameters of nanofibers from 243.84 nm to 560.55 nm, an enhancement of water vapor permeability, a decrease of the crystallinity , and a hydrophilic surface after the incorporation. FTIR and thermal analysis showed that the thermal stability was also improved due to the formation of hydrogen bonds between THY/HPβCD inclusion complexes and CS/PCL nanofibers. The developed films obtained a long-term continuous release of THY during 240 h, and had a good antifungal activity in vitro and in vivo . The above results indicated the promising prospects of SBS in developing antifungal nanofibrous films for postharvest fruit.

Biomineralized tetramethylpyrazine-loaded PCL/gelatin nanofibrous membrane promotes vascularization and bone regeneration of rat cranium defects

Wu Xiaoyu,Ni Su,Dai Ting,Li Jingyan,Shao Fang,Liu Chun,Wang Jiafeng,Fan Shijie,Tan Yadong,Zhang Linxiang,Jiang Qiting,Zhao Hongbin

Conventional electrospinning produces nanofibers with smooth surfaces that limit biomineralization ability. To overcome this disadvantage, we fabricated a tetramethylpyrazine (TMP)-loaded matrix-mimicking biomineralization in PCL/Gelatin composite electrospun membranes with bubble-shaped nanofibrous structures. PCL/Gelatin membranes (PG), PCL/Gelatin membranes containing biomineralized hydroxyapatite (HA) (PGH), and PCL/Gelatin membranes containing biomineralized HA and loaded TMP (PGHT) were tested. In vitro results indicated that the bubble-shaped nanofibrous surface increased the surface roughness of the nanofibers and promoted mineralization. Furthermore, sustained-release TMP had an excellent drug release efficiency. Initially released vigorously, it reached stabilization at day 7, and the slow-release rate stabilized at 61.0 ± 1.8% at 28 days. All membranes revealed an intact cytoskeleton, cell viability, and superior adhesion and proliferation when stained with Ghost Pen Cyclic Peptide, CCK-8, cell adhesion, and EdU. In PGHT membranes, the osteogenic and vascularized gene expression of BMSCs and human vascular endothelial cells was significantly upregulated compared with that in other groups, indicating the PGHT membranes exhibited an effective vascularization role. Subsequently, the membranes were implanted in a rat cranium defect model for 4 and 8 weeks. Micro-CT and histological analysis results showed that the PGHT membranes had better bone regenerative patterns. Additionally, the levels of CD31 and VEGF significantly increased in the PGHT membrane compared with those in other membranes. Thus, PGHT membranes could accelerate the repair of cranium defects in vivo via HA and TMP synergistic effects. Graphical Abstract

Electrospun core–sheath PCL nanofibers loaded with nHA and simvastatin and their potential bone regeneration applications

Chenghui Qian,Yubo Liu,Si Chen,Chenyang Zhang,Xiaohong Chen,Yuehua Liu,Ping Liu

Introduction: Drugs and biocompatible nanoparticles have raised significant potential in advancing the bone regeneration. Electrospinning technology enables the full realization of the value of drugs and nanoparticles. Methods: In this study, we have successfully fabricated core–sheath nanofibers solely composed of polycaprolactone (PCL) polymer. Simvastatin (SIM) was confined to the core of the nanofibers while nanohydroxyapatite (nHA) was loaded on the nanofiber surface. Results: All the prepared nanofibers exhibited a cylindrical micromorphology, and the core–sheath structure was exploited using a Transmission Electron Microscope. X-ray pattern results indicated that SIM was in an amorphous state within nanofibers, while Fourier Transform InfraRed spectroscopy showed excellent chemical compatibility among SIM, nHA, and PCL. The actual loading of nHA within the nanofiber was determined by a thermogravimetric test due to the high melting point of nHA. Core–sheath nanofibers could release SIM for 672 h, which was attributed to the core–sheath structure. Furthermore, nanofibers loaded with SIM or nHA had a positive impact on cell proliferation, with the core–sheath nanofibers displaying the most favorable cell proliferation behavior. Discussion: Such a synergistic facilitation strategy based on materials and nanostructure may encourage researchers to exploit new biomedical materials in future.

Hypoxia-Mimicking Microenvironment Scaffold for Enhanced Tendon Regeneration

Xiaoqing Cheng,Xing Yun,Yu Wei,Pengtao Shi,Ruichao He,Chen Yang,Lujian Liao,Min Wei,Qi Quan

Tendon, a connective tissue structure, serves the crucial role of transmitting force between muscles and bones. However, tendon injury repair continues to pose a significant challenge in clinical settings. In this study, we utilized single-cell RNA sequencing to delve into the cell populations and signaling pathways that are integral to tendon healing. Our findings suggest that hypoxia plays a pivotal role in activating macrophages, stimulating endothelial cell migration, and fostering fibroblast proliferation. Based on these insights, we have developed a PCL scaffold coated with DFOA, which effectively mimics a hypoxic environment to enhance tendon tissue regeneration. Furthermore, the PCL-DFOA scaffolds exhibit exceptional ability in promoting macrophage polarization and angiogenesis. This research offers a therapeutic strategy that harnesses the regenerative power of hypoxia to accelerate and optimize tendon healing processes.