PEST MANAGEMENT SCIENCE
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2024
·Vol. 80
·期 10
·4950-4958
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IF 3.8
Penglong Wang,Qi Liu,Xu Wang,Tiancong Sun,Boxing Liu,Bohan Wang,Huan Li,Chuanwen Wang,Weiwei Sun,Baoliang Pan
BACKGROUND Dermanyssus gallinae , the poultry red mite (PRM), is a worldwide ectoparasite posing significant economic challenges in poultry farming. The extensive use of pyrethroids for PRM control has led to the emergence of pyrethroid resistance. The objective of this study is to detect the pyrethroid resistance and explore its associated point mutations in the voltage-gated sodium channel (VGSC) gene among PRM populations in China. RESULTS Several populations of D. gallinae , namely CJF-1, CJP-2, CJP-3, CSD-4 and CLD-5, displayed varying degrees of resistance to beta-cypermethrin compared to a susceptible field population (CBP-5). Mutations of VGSC gene in populations of PRMs associated with pyrethroid resistance were identified through sequencing its fragments IIS4–IIS5 and IIIS6. The mutations I917V, M918T/L, A924G and L925V were present in multiple populations, while no mutations were found at positions T929, I936, F1534 and F1538. CONCLUSION The present study confirmed the presence of extremely high levels of pyrethroid resistance in PRM populations in China, and for the first time detected four pyrethroid resistance mutations in the VGSC gene. Identifying pyrethroid resistance in the field population of PRM in China can be achieved through screening for VGSC gene mutations as an early detection method. Our findings underscore the importance of implementing chemical PRM control strategies based on resistance evidence, while also considering the management of acaricide resistance in the control of PRMs. © 2024 Society of Chemical Industry.
研究领域:
昆虫学
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分子生物学
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害虫管理
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
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2024
·
IF 3.8
Lingling Huang,Guangqi Jiang
BACKGROUND Widespread application of controlled-release pesticide delivery systems is a feasible and effective method to improve the utilization efficiency of pesticides. However, owing to the high cost and complicated preparation technologies of controlled-release pesticide delivery systems, their applications in agricultural production have been seriously hindered. RESULTS This study aimed to construct inexpensive photothermally controlled-release pesticide delivery systems using chitosan (CS) and sodium lignosulfonate (LS) as the wall materials, and a coordination assembly strategy of LS with transition metal ions to encapsulate a model pesticide, avermectin (AVM). The resulting complex or nanoparticle photothermal layers in these systems effectively achieved photothermal conversions, and replaced the use of common photothermal agents. In the prepared pesticide-delivery systems, two systems had remarkable photothermal conversion performance and photothermal stabilities with a photothermal conversion efficiency ( η ) of 24.03% and 28.82%, respectively, under 808 nm, 2 W near-infrared irradiation. The slow-release and ultraviolet-shielding performance of these two systems were markedly enhanced compared with other formulations. The insecticidal activities of these two systems against Plutella xylostella under irradiation with light-emitting diode (LED)-simulated sunlight were also enhanced by 5.20- and 5.06-fold, respectively, compared with that without irradiation of LED-simulated sunlight. CONCLUSION Because of their convenient preparations, inexpensive and renewable raw materials, and excellent photothermally controlled-release performance, these on-demand pesticide delivery systems might have significant potential in improving the utilization efficiency of pesticides in modern agriculture. © 2024 Society of Chemical Industry.
研究领域:
农业科学
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材料科学
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环境科学
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PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·Vol. 80
·期 8
·3884-3892
·
IF 3.8
Sijia Zhang,Zhenggang Xiao
BACKGROUNDS Pesticides have been widely applied to promote plant growth due to their high insecticidal efficiency. However, most pesticides easily slip from the leaf surface due to the low foliar adhesion, leading to significant damage to the environment and human health. Increasing foliar adhesion of pesticides has been a major challenge. Nanomaterial technology, which can deliver pesticide active ingredients, offers a new opportunity to increase pesticides efficacy with higher foliar adhesion. RESULTS In this study, we prepared the pesticide loaded nanohydrogel ALP@Ge-g-P(AA-co-AM)/ATP by grafting attapulgite and gelatin onto P(AA-co-AM), and loading the pesticide alphacypermethrin synchronously via the emulsion solvent evaporation method. The size of the new nanohydrogel was 20.5 nm and the pesticide loading capacity was 21.5%. Compared to P(AA-co-AM), the water absorbency of Ge-g-P(AA-co-AM)/ATP increased by 54.3% in 120 min, while the water retention increased from 4.27% to 38.7% after 48 h. Washout experiments and contact angle results suggested that the nanohydrogel, with a contact angle of 44.8° indicating good wettability, can resist rain flush and increase foliar adhesion naturally. CONCLUSIONS Overall, the preparation of pesticide loaded nanohydrogel is simple. Experiments demonstrated the pesticide loaded nanohydrogel has high efficiency and strong foliar adhesion. This makes it a promising candidate for spraying application and reducing pollution levels. © 2024 Society of Chemical Industry.
研究领域:
农业科学
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材料科学
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环境科学
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Qiulan Xia,Maoyang Ran,Lihe Zhou,Zhongwei Liu,Lin Cai
BACKGROUND Ralstonia solanacearum , a notorious and refractory bacterial plant pathogen, threatens multiple vegetable crops and causes significant economic loss in agriculture. Long-term use of traditional medicines not only increases the problem of drug resistance, but also causes great environmental pollution. Therefore, there is an urgent need to develop new agents with high efficacy and low toxicity. RESULTS In this study, we have synthesized and characterized graphitic carbon nitride incorporated copper oxide composite (g-C 3 N 4 @CuO), which showed higher antimicrobial effect than graphitic carbon nitride nanosheets (g-C 3 N 4 nanosheets) and copper oxide nanoparticles (CuONPs). Ralstonia solanacearum exposed to g-C 3 N 4 @CuO exhibited higher levels of oxygen toxicity, cell membrane damage, DNA damage, motility disruption and even cell death compared to g-C 3 N 4 nanosheets and CuONPs. In addition, g-C 3 N 4 @CuO was more effective in the control of tobacco bacterial wilt than g-C 3 N 4 nanosheets and CuONPs. CONCLUSION Thus, this study provides a new perspective on g-C 3 N 4 @CuO control of bacterial diseases in crops, and the mechanism is related to the destruction of cell membrane damage and motility disruption. © 2024 Society of Chemical Industry.
研究领域:
植物病理学
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微生物学
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农业科学
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Liwang Fei,Rahila Hafeez,Junliang Zhang,Shiquan Fu,Ying Xu,Lingyun Hao
BACKGROUND Rice blast, caused by Pyricularia oryzae , is a devastating fungal disease threatening global rice production. Overreliance on chemical fungicides has raised environmental concerns and led to resistant strains, necessitating the development of sustainable alternatives. This study integrated marine microbiology and natural antifungal compounds to create eco-friendly alternatives to chemical fungicides for disease management. RESULTS We identified Pseudomonas aeruginosa R64 with broad-spectrum antimicrobial activity from mangrove soil in the Mai Po Nature Reserve. The R64 fermentation extract (RFE) exhibited multifaceted inhibition of P. oryzae , suppressing mycelial growth, conidiation, conidial germination and appressorial formation, while disturbing cell wall and membrane function. It also attenuated virulence by impairing appressorial penetration and invasive growth. Further chemical analysis identified phenazines and quinolines as the primary compounds in RFE, corroborated by PCR detection of corresponding phenazine biosynthetic gene clusters. Comparative bioassays with two main bioactive components of RFE, phenazine-1-carboxamide (PCN) and phenazine-1-carboxylic acid (PCA), against P. oryzae implicated PCN as the principal antifungal effector. RFE and PCN had higher efficacy than tricyclazole in P. oryzae growth inhibition, but were less effective than isoprothiolane. Furthermore, RFE and PCN displayed lower acute ecotoxicity to an environmental indicator organism than isoprothiolane, suggesting their potential as sustainable biopesticides for rice blast management. CONCLUSION Natural products from mangrove soil bacterium P. aeruginosa R64 inhibited key developmental and infection processes of P. oryzae , effectively reducing rice blast development. The promising disease inhibition and low ecotoxicity of mangrove-associated bacteria highlight their untapped potential for innovative, eco-friendly fungicide mining for sustainable agriculture. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
研究领域:
植物病理学
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微生物学
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农业科学
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Yongjun Li,Jiameng Peng,Haiying Li,Ruiqi Zhang,Jiexia Chen,Xiuying Hou,Guang Yang
BACKGROUND The incompatible insect technique (IIT) has been used for Aedes mosquito population suppression to curb the transmission of dengue. However, its wide application is limited owing to the low output of male mosquitoes and the risk of population replacement from the release of fertile Wolbachia -infected females. This study aims to improve IIT efficiency for broader adoption. RESULTS We assessed the impact of 10% pyriproxyfen (PPF) sticky powder exposure on Wolbachia (from Culex molestus )-transinfected Aedes albopictus Guangzhou line (GUA line) (GC) mosquitoes. We found that the exposure caused chronic toxicity in adult mosquitoes without affecting the cytoplasmic incompatibility (CI)-inducing capability of males. The PPF-contaminated GC females exhibited significant sterilization and the ability to disseminate lethal doses of PPF to breeding sites. Subsequently, we conducted a field trial combining PPF with IIT aiming to suppress the Ae. albopictus population. This combined approach, termed boosted IIT (BIIT), showed a notable enhancement in population suppression efficiency. The improved efficacy of BIIT was attributed to the dispersion of PPF particles in the field via the released PPF-contaminated male mosquitoes. During the BIIT field trial, no Wolbachia w Pip-positive Ae. albopictus larvae were detected, indicating the effective elimination of the risk of Wolbachia -induced population replacement. Additionally, the field trial of BIIT against Ae. albopictus resulted in the suppression of the nontarget mosquito species Culex quinquefasciatus . CONCLUSION Our results highlight the remarkable efficiency and feasibility of combining IIT with PPF in suppressing mosquito populations, facilitating the widespread implementation of IIT-based management of mosquito-borne diseases. © 2024 Society of Chemical Industry.
研究领域:
医学昆虫学
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公共卫生
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病原传播控制
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Xinyi Wang,Wenhong Li,Xiang Yang,Mingwei Yang,Yucheng Gu,Zhao Du,Jingyi Yang,Mingxia Wen,Yoonseong Park,Chunyang Huang,Yueping He
BACKGROUND Widespread resistance of insect pests to insecticides and transgenic crops in the field is a significant challenge for sustainable agriculture, and calls for the development of novel alternative strategies to control insect pests. One potential resource for the discovery of novel insecticidal molecules is natural toxins, particularly those derived from the venoms of insect predators. RESULTS In this study, we identified three insecticidal proteinaceous toxins from the venom glands (VGs) of the predatory stink bug, Arma custos (Hemiptera: Asopinae). Transcriptomic analysis of A. custos VGs revealed 151 potentially secreted VG-rich venom proteins. Three VG-rich venom proteins (designated AcVP1 ~ 3) were produced by overexpression in Escherichia coli . Injection of the recombinant proteins into tobacco cutworm ( Spodoptera litura ) larvae showed that all of the three recombinant proteins caused paralysis, liquefaction and death. Injection of recombinant proteins into rice brown planthopper ( Nilaparvata lugens ) nymphs showed higher insecticidal activities, among which a trypsin (AcVP2) caused 100% mortality postinjection at 1.27 pmol mg −1 body weight. CONCLUSION A natural toolkit for the discovery of insecticidal toxins from predatory insects has been revealed by the present study. © 2024 Society of Chemical Industry.
研究领域:
昆虫学
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分子生物学
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农业科学
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Jia Lin,Guoqing Yue,Jun Chen,Kang Xiao,Qing'e Ji
Background Fopius arisanus (Sonan) is an important egg–pupal endoparasitic wasp of Bactrocera dorsalis (Hendel). As traditional method of sorting nonparasitic B. dorsalis from parasitic wasps is labor-intensive, requires specific equipment and poses the risk of spreading fertile hosts, the development of a more convenient, economical and safe sorting procedure is important. Results The optimal cyromazine emergence inhibition procedure (CEIP) involved facilitating the pupation of B. dorsalis mature larvae (Bdml) in 3 mg kg −1 cyromazine sand substrate (CSS) for 48 h. When the Bdml that had been exposed to F. arisanus during the egg stage were treated with 3–7 mg kg −1 CSS for 48 h, no negative effects on the emergence parameters of parasitoids were observed. Treatment with 3–4 mg kg −1 CSS had insignificant effects on the biological and behavioral parameters of F. arisanus . However, treatment with 5–6 mg kg −1 CSS adversely affected the fecundity and antennating activity of the wasps; specifically, 6 mg kg −1 CSS negatively affected the lifespan and flight ability of wasps. Fortunately, no transgenerational effects on these parameters were observed in the progeny. Regarding the nutrient reserves of both sexes of F. arisanus , significant dose-dependent effects were observed. Moreover, 5–6 mg kg −1 CSS significantly reduced the protein and carbohydrate content in F. arisanus ; in particular, 6 mg kg −1 CSS notably reduced the lipid content. Conclusion CEIP provides a more flexible, economical and safe mass-release program for F. arisanus . In addition, it has profound implications for the biological control of other dipteran pests. © 2024 Society of Chemical Industry.
研究领域:
昆虫学
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害虫管理
·
生态学
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Xiangying Zou,Jiayu Jia,Tengfei Zhu,Shouping Cai,Yiman He,Shunde Su,Yu Fang,Jian Li,Guifang Lin,Jun Su
BACKGROUND Pine wood nematode (PWN; Bursaphelenchus xylophilus ) is the causative agent of pine wilt disease (PWD), which is considered the most dangerous biohazard to conifer trees globally. The transmission of PWN relies on insect vectors, particularly the Japanese pine sawyer (JPS; Monochamus alternatus ). However, the molecular mechanism underlying PWN-JPS assembly remains largely unknown. RESULTS Here, we found that both geographical and gender could significantly affect the PCA (PWN carrying amount) of JPS; thus, JPS transcriptomes from diverse locations and genders were explored regard to PWN loading. Due to the shortage of genomes, we developed a full-length reference transcriptome for analyzing next-generation sequencing data. A comparative genomic study was performed, and 11 248 potential PWN-carrying associate genes (β) were nominated in JPS by using the reported genomes of PWN and non-PWN carrier insect species. Then, 151 differentially expressed transcripts (DETs), 28 of them overlapped with β, correlated with the PCA of JPS were nominated by RNA-Seq, and found that fatty acid β-oxidation might be the key factor that affected the PCA of JPS. Furthermore, JPS fatty acid β-oxidation rates were experimentally decreased using the inhibitor Etomoxir, leading to an increased PCA of JPS. Meanwhile, silencing MaCPT1 in JPS by RNA interference led to a decreased fatty acid β-oxidation rate and increased PCA of JPS. CONCLUSIONS In conclusion, MaCPT1 was able to decrease the PWN-JPS assembly formation through the fatty acid β-oxidation of JPS. These results provide new insights for exploring the impact of PWN invasion on JPS. © 2024 Society of Chemical Industry.
研究领域:
植物病理学
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昆虫学
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分子生物学
·
基因组学
·
转录组学
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Shan-Cheng Yi,Xin-Hui Chen,Yu-Hang Wu,Juan Wu,Jia-Qing Wang,Man-Qun Wang
BACKGROUND The important wood-boring pest Batocera horsfieldi has evolved a sensitive olfactory system to locate host plants. Odorant-binding proteins (OBPs) are thought to play key roles in olfactory recognition. Therefore, exploring the physiological function of OBPs could facilitate a better understanding of insect chemical communications. RESULTS In this research, 36 BhorOBPs genes were identified via transcriptome sequencing of adults' antennae from B. horsfieldi , and most BhorOBPs were predominantly expressed in chemosensory body parts. Through fluorescence competitive binding and fluorescence quenching assays, the antenna-specific BhorOBP28 was investigated and displayed strong binding affinities forming stable complexes with five volatiles, including (+)-α-Pinene, (+)-Limonene, β-Pinene, (−)-Limonene, and (+)-Longifolene, which could also elicit conformation changes when they were interacting with BhorOBP28. Batocera horsfieldi females exhibited a preference for (−)-Limonene, and a repellent response to (+)-Longifolene. Feeding dsOBP19 produced by a bacteria-expressed system with a newly constructed vector could lead to the knockdown of BhorOBP28 , and could further impair B. horsfieldi attraction to (−)-Limonene and repellent activity of (+)-Longifolene. The analysis of site-directed mutagenesis revealed that Leu7, Leu72, and Phe121 play a vital role in selectively binding properties of BhorOBP28. CONCLUSION By modeling the molecular mechanism of olfactory recognition, these results demonstrate that BhorOBP28 is involved in the chemoreception of B. horsfieldi . The bacterial-expressed dsRNA delivery system gains new insights into potential population management strategies. Through the olfactory process concluded that discovering novel behavioral regulation and environmentally friendly control options for B. horsfieldi in the future. © 2024 Society of Chemical Industry.
研究领域:
昆虫学
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分子生物学
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化学生态学
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昆虫生理学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2026
·
IF 4.2
Rui Zhang,Tongfang Jing,Peng Yi,Kaidi Cui,Leiming He,Xuewei Mao,Yuying Song,Lin Zhou
BACKGROUND Sclerotium rolfsii is a destructive soil-borne fungal pathogen distributed worldwide. Although n -butylidenephthalide (BP) exhibits strong inhibitory activity against S. rolfsii , its agricultural application is hampered by high volatility and poor water solubility. RESULTS To address these barriers, a pathogen-triggered delivery system was engineered using tea polyphenol–iron nanocapsules (TP@Fe NCs). The 277 nm core-shell capsules exhibited enhanced thermal stability and specific responsiveness to S. rolfsii , discharging 86.0% of the cargo under 1.0 m m oxalic acid (OA). In vitro assays confirmed superior bioactivity, with TP@Fe NCs exhibiting a significantly lower median lethal concentration (LC 50 ) of 13.34 mg/L compared to 21.53 mg/L for free BP. In glasshouse trials against peanut southern blight, TP@Fe NCs (300 mg/L) achieved a curative efficacy of 74.68%, significantly outperforming free BP (51.30%). Crucially, the formulation demonstrated excellent biosafety. Peanut seedlings treated with 200–300 mg/L TP@Fe NCs showed no phytotoxicity, maintaining emergence rates of 92.69% and normal root/stem development. Furthermore, acute toxicity tests on zebrafish (4.2–5.0 mg/L) revealed that while 5.0 mg/L free BP caused 100% mortality within 48 h, TP@Fe NCs maintained a > 90% survival rate over 96 h. CONCLUSION This study presents a biorational strategy for precise fungal control and develops an expandable and efficient framework for implementing eco-friendly nanopesticide systems toward sustainable agriculture. © 2026 Society of Chemical Industry.
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2026
·
IF 4.2
XiGuang Wang,XinYi Huang,YanJun Li,ShengHe Ye,GuoLiang Wu,JingYa Yuan,MingWei Gao,JiaYing Liu,LiYan Song,QingWei Tan,QiJian Chen,JiangTao Gao,BinBin Huang
BACKGROUND Fusaric acid (FA), a non-host-selective toxin with antifungal and insecticidal activities, has attracted attention as a promising lead structure for developing novel agrofungicides. This study aimed to design and synthesize FA derivatives with improved antifungal activity and reduced phytotoxicity, and to investigate their antifungal mechanism and structure–activity relationships. RESULTS A series of FA derivatives were synthesized and evaluated against six major plant pathogenic fungi, including Magnaporthe oryzae . Several derivatives showed enhanced antifungal activity compared with the previously identified scaffold H52 , and 10 compounds completely inhibited M. oryzae at 100 μg mL −1 under in vitro conditions. The most active derivatives exhibited half-maximal effective concentration (EC 50 ) values of 6.09–9.84 μg mL −1 . Phytotoxicity assays showed that compounds A6 , A10 , A12 , B4 , B6 , and B7 had safety profiles comparable to the commercial fungicide azoxystrobin. Compound B4 further demonstrated concentration-dependent protective activity against rice blast under pot conditions, reducing the disease index from approximately 66.7 in the untreated control to about 14.8 at the highest tested concentration. Mechanistic studies revealed severe hyphal deformation, cell wall disruption, intracellular leakage, and reactive oxygen species (ROS) accumulation following B4 treatment. A comparative molecular field analysis-based three-dimensional quantitative structure–activity relationship (3D-QSAR) model exhibited strong predictive performance ( q 2 = 0.69, r 2 = 0.992), indicating reliable structure–activity relationships. Contour map analysis suggested that bulky substituents near the core ring and electron-withdrawing groups in specific regions significantly enhance antifungal activity. CONCLUSION FA derivatives can be rationally optimized to improve antifungal activity, reduce phytotoxicity, and be effective in planta disease control, providing promising candidates and design strategies for next-generation agrofungicides. © 2026 Society of Chemical Industry.
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PubMed
高影响因子
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