JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-22
·
IF 12.6
Liu Yanhong,Gong Liming,Feng Jing,Xiao Congcong,Liu Chenfei,Chen Bohan,Chen Liqing,Jin Mingji,Guan Youyan,Gao Zhonggao,Huang Wei
Immune checkpoint inhibition (ICI) has become the mainstay of immunotherapy for the treatment of renal cell carcinoma (RCC). However, only a small portion of patients exhibit a positive response to PD-1/PD-L1 blockade therapy and the key reason is that RCC belongs to a vascular-rich tumor for promoting immunosuppression. Specifically, the dysfunctional tumor vasculature hinders effector T cell infiltration and induces immunosuppressive tumor microenvironment via the release of cytokine, which attenuates the therapeutic efficacy of ICI. Therefore, regulating abnormal tumor vasculature may be a promising strategy to overcome the immunosuppressive microenvironment and enhance ICI therapy. Here, we propose an NGR peptide-modified actively targeted liposome (Axi/siRNA PD−L1 @NGR-Lipo) to encapsulate the anti-angiogenic agents Axitinib and PD-L1 siRNA to promote tumor vasculature normalization and relieve immune evasion for enhanced anti-tumor immunotherapy. With NGR-mediated tumor homing and active targeting, Axi/siRNA PD−L1 @NGR-Lipo could act on tumor vascular endothelial cells to inhibit neo-angiogenesis, increase pericyte coverage and vascular perfusion, and normalize the structure and function of tumor blood vessels. Meanwhile, it also enhanced immune effector T cells and NK cells infiltration and reduced the proportion of immunosuppressive T cells including MDSC cells and Tregs, thus improving the tumor immunosuppressive microenvironment. Moreover, Axi/siRNA PD−L1 @NGR-Lipo reduced the expression of PD-L1 protein in tumor cells, restored the recognition and killing ability of cytotoxic T cells, and relieved immune evasion. As expected, Axi/siRNA PD−L1 @NGR-Lipo displayed superior anti-tumor and anti-metastatic efficacy in mice bearing RCC. Overall, this study demonstrated the important potential of regulating abnormal tumor vasculature to reshape the immunosuppressive microenvironment and boost ICI therapy, which represents a promising avenue for the synergistic anti-tumor with cancer immunotherapy. Graphical
研究领域:
肿瘤学
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免疫学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2024
·
IF 4.1
Keyan Liu,Wei Feng,Yanru Li,Donglai Han,Tianna Wu,Kaiyue Li,Shuo Yang
Surface-enhanced Raman spectroscopy (SERS) with enormous advantages has emerged as a powerful tool for both highly sensitive structural detection of analytes and various imaging applications. A notable advancement in the realm of multifunctional SERS substrates is the integration of magnetic nanoparticles endowed with magnetic manipulation capabilities and noble metal nanoparticles exhibiting plasmonic resonance properties. This synergistic combination offers substantial practical benefits for trace analysis across diverse fields. In this paper, a new paradigm of Fe 3 O 4 @PEI-DTC-Ag (Fe 3 O 4 @PD-Ag) photomagnetic nanocomposites based on magnetic Fe 3 O 4 nanoparticles (NPs), the noble metal silver quantum dots (Ag QDs) and the adhesive layer polyethyleneimine dithiocarbamate (PEI-DTC) for convenient recycling and ultrasensitive SERS detection was developed by engineering hydrothermal and seed-growth methods. The SERS performance of the Fe 3 O 4 @PD-Ag substrates with varying amounts of Ag was evaluated by detecting 4-aminothiophenol (4-ATP). Among the tested samples, Fe 3 O 4 @PD-Ag-4 with a silver solution volume of 200 ml exhibited the optimal performance, showcasing an enhancement factor ( EF ) of 4.418 × 10 5 . Moreover, the relative standard deviations (RSDs) were calculated to be less than 11.5 %, demonstrating the SERS substrates exhibit good uniformity and spectral reproducibility. Of note, the SERS substrate has demonstrated successful trace detection of thiram molecules, achieving a limit of detection (LOD) of up to 10 -9 M. The intensity of SERS signals shows a linear correlation with the logarithm of thiram concentration, ranging from 10 -4 to 10 -9 M, thus providing compelling evidence for quantitative detection. Importantly, the Fe 3 O 4 @PD-Ag-4 SERS substrate also showcases outstanding performance for detecting thiram in real food, underscoring its significant potential for application in food safety monitoring.
研究领域:
分析化学
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食品安全
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纳米技术
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光谱学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2024
·
IF 4.1
Jianguo Wu,Xin Li,Kun Fu,Dong Cao,Daojian Cheng
Pd-based catalysts have been widely applied in selective hydrogenation of phenol. However, avoiding excessive hydrogenation of C=O functional groups is still challenging. Here, we synthesized Pd nanoclusters supported by N-doped C-Al 2 O 3 (Pd/CN-Al 2 O 3 ) catalyst for phenol hydrogenation. Remarkably, the cyclohexanone yield shows a volcano trend with the doping of carbon content, where Pd/CN-Al 2 O 3 with carbon content of 6.76 % (Pd/CN-Al 2 O 3 -2) exhibits the highest cyclohexanone yield. Under the optimized conditions, the phenol conversion and cyclohexanone selectivity over Pd/CN-Al 2 O 3 -2 are 99.5 % and 96.7 %, respectively. The corresponding TOF value is 1731.7 h −1 , which is 5.8 times higher than that of Pd/Al 2 O 3 (299.7 h −1 ). Mechanism studies reveal the excellent catalytic performance of Pd/CN-Al 2 O 3 -2 is mainly attributed to the synergistic effect of electron-deficient Pd clusters and Lewis acid sites. Pd nanoclusters with moderate electron-deficient state and moderate Lewis acid density are beneficial to the adsorption of phenol and the desorption of cyclohexanone, thereby exhibiting the best phenol conversion and cyclohexanone selectivity. This work provides a carbon modification strategy to regulate the structure of Pd-based catalysts, which is a helpful guide for the hydrogenation reactions.
研究领域:
催化化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Materials Today Bio
·
2025
·
IF 10.2
Wenjin Zhong,Pengcheng Wang,Jintian Wang,Yuejia Zhu,Liquan Chen,Yilong Qian,Xiang Huang,Kai Ye
Background The immune checkpoint inhibitor (ICI) PD-1α can effectively treat Lynch syndrome (LS)-associated colorectal cancer (CRC) (LS-CRC). In some cases, LS-CRC is not sensitive to ICIs. Dendritic cell (DC)-based hybrid membrane nanoparticles have great potential to upgrade the efficacy of ICIs, which can theoretically be applied to enhance the efficacy of ICIs in treating LS-CRC. Methods MnO 2 nanomaterials with si-CTLA4 were absorbed by coating mature DCs loaded with PD-1α and liposome hybrid membranes. The morphology and physicochemical properties of DCsLipo@MnO 2 @si-CTLA4@PD-1α were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), fluorescence resonance energy transfer (FRET), and Fourier transform infrared spectroscopy (FTIR). The activation of DCs and T cells was assessed by utilizing the detection of cell surface markers by flow cytometry. The mRNA levels of CTLA4 in T cells were measured using the qPCR method. The biosafety of DCsLipo@MnO 2 @si-CTLA4@PD-1α was proved by hemolysis test in vitro , CCK-8 assay, and live/dead cell staining. In vitro T-cell uptake experiments proved that the nanomedicine can be effectively absorbed by T cells. Cell proliferation, apoptosis, and ELISA experiments demonstrated that nanomedicine facilitated T-cell proliferation, cytokine secretion, and cancer cytotoxicity. Finally, the anti-cancer effect and immune activation ability of the nanomedicine against LS-CRC were verified by a mouse allograft model. Results The nanomedicine DCsLipo@MnO 2 @si-CTLA4@PD-1α created in this research exhibited good stability and uniform vesicle morphology. In vitro hemolysis test, CCK-8 assay, and live/dead staining experiments indicated that DCsLipo@MnO 2 @si-CTLA4@PD-1α had great biosafety. The nanoparticle loaded with si-CTLA4 effectively reduced the mRNA expression of CTLA4 in T cells. Meanwhile, the results of T cell flow analysis, cell proliferation, cytotoxicity, apoptosis, and ELISA experiments manifested that DCsLipo@MnO 2 @si-CTLA4@PD-1α effectively reinforced the proliferation and activation of T cells, and enhanced the cytotoxicity of T cells to cancer cells. The validation experiments based on the mouse allograft tumor model manifested that DCsLipo@MnO 2 @si-CTLA4@PD-1α boosted the infiltration of T cells into tumor tissues and suppressed the development of LS-CRC. Conclusion DCsLipo@MnO 2 @si-CTLA4@PD-1α can facilitate T cell activation and synergistically enhance the efficacy of ICIs, exhibiting a great inhibitory effect on LS-CRC.
研究领域:
肿瘤学
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免疫学
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生物医学工程
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Jinglei Cui,Yujun Cui,Jingjing Tan,Huirong Zhang,Mengyuan Gu,Long Huang
Volatile organic compounds (VOCs) are one of the main pollutants produced in industrial processes. Catalytic oxidation is an effective method to eliminate VOCs, and the key lies in designing efficient metal catalyst. In this work, Pd-Pt/SiO 2 bimetallic catalysts were designed and prepared by the immobilization method, and their performances for toluene oxidation were evaluated. The addition of Pt into the catalyst elevated markedly the reduction of Pd active sites, leading to abundant electron-deficient Pd 0 and electron-rich Pt 0 in the Pd 2 Pt 1 catalyst, which were confirmed by TEM, CO pulse adsorption and in-situ XPS. As revealed by O 2 -TPD and toluene-TPD characterization, the cooperative effect of electron-deficient Pd 0 and electron-rich Pt 0 in Pd 2 Pt 1 catalyst facilitated the activation of O 2 and toluene, thus enhanced greatly its activity. A conversion of 90% (T 90 ) for toluene was achieved over Pd 2 Pt 1 catalyst at 165 ℃, and the apparent activation energy ( E a ) over Pd 2 Pt 1 catalyst was lower than that over other Pd-Pt bimetallic and monometallic Pd or Pt catalysts. Additionally, the reaction mechanism was studied by In - situ DRIFTS, the results showed that the toluene oxidation passed successively benzyl alcohol, benzaldehyde, benzoic acid and maleic anhydride , then maleic anhydride was further oxidized to CO 2 and water.
研究领域:
环境科学
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催化化学
✓ 已验证
产品关联
DOI
高影响因子
npj Precision Oncology
·
2025
·Vol. 9
·期 1
·1-15
·
IF 8
Huo Ran,Xu Quan-guo,You Yi-qing,Chen Yan-lin,Su Guang-jian,Yang Kun-rong,Xiao Yan-ping,Xue Zhong,Li Yang-jin,Sun Pei,Cui Zhao-lei,Lin Ying-ying,Guo Jun-ying,Xu Hai-yan,Chen Zhao-shuo,Xie Wen-ting,Xu Shao-hua,Chen Min-yong,Wu Jing,He Shi-jie,Xiao Zhen-zhou,Chen Yan
Hepatocellular carcinoma (HCC) is a prevalent and deadly cancer. Gut microbiota affect tumor immunity and immunotherapy efficacy, but the exact mechanisms are unclear. A study compared gut microbiota in HCC patients and healthy controls (HC) using 16S rDNA analysis and metabolomics. It found higher levels of Bifidobacterium and a positive correlation between Bifidobacterium and isobutyric acid in HC group. Animal models showed that the combination of HC fecal matter and αPD-1 treatment reduced tumor volume more effectively than the combination of HCC fecal matter and αPD-1. The combination of Bifidobacterium or isobutyrate with αPD-1 also decreased tumor size. In vitro, isobutyrate-stimulated CD8 + T cells increased IFN-γ secretion, suppressed liver cancer cells, and downregulated the JAK/STAT3 pathway. Combined treatments increased CD8 + T cells and IFN-γ levels and reduced JAK/STAT3 signaling in the tumor microenvironment. This suggests that Bifidobacterium and isobutyrate can inhibit tumor growth, offering insights into gut microbiota-host interactions and potential strategies to overcome HCC immunotherapy resistance.
研究领域:
癌症研究
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免疫学
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微生物学
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分子生物学
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胃肠病学
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生物信息学
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药理学
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2024
·
IF 20.2
Kai Deng,Zilong Lian,Wenxin Wang,Jiabao Yu,Qiqi Mao,Hongjie Yu,Ziqiang Wang,Liang Wang,Hongjing Wang
Despite the fruitful achievements in the development of high-performance catalysts, most of materials still suffer limited HER kinetics and poor durability in harsh acidic media, especially at high current densities . In this work, a Pd nanoparticles/Ir metallene heterojunction structure (Pd/Ir hetero-metallene) were successfully prepared by a facile co-reduction method. The hydrogen spillover bridging H + adsorption/H 2 desorption process occurring at the Pd/Ir interface results in the rate-determining step change to a Tafel step, which accelerates the HER kinetics. The Pd/Ir hetero-metallene has a relatively low Δ G Hads (-0.19 eV), which allows for rapid adsorption of H + and release of H 2 , thus greatly improving the performance of HER. As a result, the Pd/Ir hetero-metallene exhibits remarkable HER performance with ultra-low overpotentials of only 69 and 133 mV to reach 500 and 1000 mA cm −2 , a small Tafel slope of 21 mV dec −1 , and superb stability.
研究领域:
材料科学
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电化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Catalysis
·
2025
·
IF 13.1
Tingting Xiao,Jingrong Li,Jian Li,Qihang Gong,Peikai Luo,Xinluona Su,Haiyang Cheng,Chao Zhang,Fengyu Zhao
The hydrogenolysis of cellulose-derived 5-hydroxymethylfurfural is essential for the production of fuels and chemicals from biomass. Herein, we fabricated a phosphorus-doped Pd/P-TiO2 catalyst for the hydrodeoxygenation of 5-hydroxymethylfurfural to 2,5-dimethylfuran. A large number of defects and electron-deficient Ti4+ sites (dTi4+) were formed by doping phosphorus into the TiO2 support, which could promote hydrogen spillover and reactant adsorption. Moreover, the spilled active hydrogen induced the formation of P–O(H)-dTi4+, which acted as a “Brønsted acidic site” to promote the activation of C–O bonds. Consequently, the Pd/P-TiO2 catalyst exhibited superb activity for the hydrodeoxygenation of 5-hydroxymethylfurfural to 2,5-dimethylfuran at mild conditions of 150 °C under 1 MPa H2.
研究领域:
催化化学
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生物质转化
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2024
·
IF 5.8
Junqiu Liu,Zhaidong Liu,Chunqin Zhao,Yuting Jiao,Baohong Li,Jiaju Shi,Zichao Chen,Zhen Zhang
Exosomal programmed death ligand-1 (ExoPD-L1) is a vital marker of immune activation in the early stages of tumor therapy and it can inhibit anti-tumor immune responses. However, due to the low expression of ExoPD-L1 in cancer cells, it is difficult to perform highly sensitive assays and accurately differentiate cancer sources. Therefore, we constructed a coaxial dual-path electrochemical biosensor for highly accurate identification and detection of ExoPD-L1 from lung cancer based on chemical–biological coaxial nanomaterials and nucleic acid molecular signal amplification strategies. The measurements showed that the detected ExoPD-L1 concentrations ranged from 6 × 102 particles per mL to 6 × 108 particles per mL, and the detection limit was 310 particles per mL. Compared to other sensors, the electrochemical biosensor designed in this study has a lower detection limit and a wider detection range. Furthermore, we also successfully identified lung cancer-derived ExoPD-L1 by analyzing multiple protein biomarkers expressed on exosomes through the “AND” logic strategy. This sensor platform is expected to realize highly sensitive detection and accurate analysis of multiple sources of ExoPD-L1 and provide ideas for the clinical detection of ExoPD-L1.
研究领域:
生物医学工程
·
癌症生物学
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电化学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cancer Cell International
·
2022
·Vol. 22
·期 1
·1-18
·
IF 6.429
Wang Shunxi,Chen Long,Liu Wanqian
Background Ferroptosis have been implicated in tumorigenesis, tumor progression, and chemo- and immuno-therapy in cirrhotic hepatocellular carcinoma (HCC), indicating its association with matrix stiffness and clinical benefit of targeting drugs or immune checkpoint inhibitor. Here, we postulated that increased matrix stiffness reduces ferroptosis and impairs tumor immunity by regulating the expression of ferroptosis- and immune-related genes in HCC, which might be a robust predictor of therapeutic efficacy. Methods Using publicly available tissue microarray datasets, liver cancer rat model, and clinical specimen, ferroptosis-related differential genes in HCV-infected cirrhotic HCC and its mechanical heterogeneous pattern of expression were screened and identified. Further investigation on the underlying mechanism of matrix stiffness-regulated ferroptosis and the expression of immune mediator were performed. Finally, threshold analysis of HCC cases with sorafenib treatment revealed the value of clinical applications of these potential predictors. Results STEAP3 was identified as the ferroptosis-related differential genes in HCV-infected cirrhotic HCC. Stiffer matrix decreased STEAP3 in the invasive front area of HCC and the liver cirrhotic tissue. Contrarily, softer matrix induced STEAP3 in the central area of HCC and the normal liver tissue. Immunological correlation of STEAP3 in cirrhotic HCC showed that STEAP3-mediated immune infiltration of CD4+ T and CD8+ T cells, macrophages, neutrophils, and dendritic cells and HCC prognosis, predicting to regulate immune infiltration. Overexpression of STEAP3 induced ferroptosis and inhibited the expression of immune mediator of PD-L2 on a stiff matrix. Especially, the ferroptosis- and immune-related gene predictive biomarker (FIGPB), including STEAP3 and PD-L2, predicts better clinical benefit of sorafenib in HCC patients. Conclusions This finding identifies matrix stiffness impairs ferroptosis and anti-tumor immunity by mediating STEAP3 and PD-L2. More importantly, coordinated with PD-L2, matrix stiffness-dependent STEAP3 could be applied as the independent predictors to favorable sorafenib response, and thus targeting it could be a potential diagnosis and treatment strategy for HCC.
研究领域:
肿瘤学
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免疫学
·
分子生物学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Yong Xu,Yue Wang,Ping Wang,Yishun Wang,Weili Dai,Jianping Zou,Xubiao Luo
Employing electric energy to convert carbon dioxide (CO 2 ) into valuable small molecules is a potentially practical method in energy storage and greenhouse gas alleviation. A huge challenge for electrocatalytic CO 2 reduction is to reduce overpotential to improve energy efficiency. Herein, we demonstrate that doping alloy Pd 49 Ag 30 Te 4 (PAT) with rare-earth element Tb is beneficial for selective exposure of (1 1 1) crystal plane, which is a highly active crystal plane for producing carbon monoxide (CO). The as-prepared Tb 2.9 PAT exhibited high electrocatalytic performance with 95.7 % CO faradic efficiency at − 0.8 V (vs RHE), far exceeding that of PAT, and coupled with good durability. In situ spectral study and theoretical calculations disclose that the introduction of Tb regulates the d-band center of PAT alloy, weakens the Pd − C bonding ability, and promotes the desorption of *CO in the rate-determining step. This study provides a method for doping induced selective exposure of crystal face, which provides new idea for improving catalytic performance.
研究领域:
材料科学
·
电化学
·
催化
✓ 已验证
DOI
PubMed
高影响因子
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
·
2025
·
IF 7.8
Ruoyi Li,Muchen Lu,Jiaqi Yu,Jian Zhang,Jia Li,Xinyan Wang
Tigecycline (TGC), a third-generation, broad-spectrum, potent antimicrobial drug based on a tetracycline structure, may pose potential hazards to ecosystems and human health due to its difficulty in natural degradation in the environment. However, the environmental degradation behavior of TGC has not been fully investigated, so it is still a major challenge to rationally design efficient, stable, environmentally friendly and cost-effective electrocatalysts for TGC degradation. In this study, Pd-ZnS NPs/CF composite electrode anchored on copper foam (CF) was synthesized by simple ultrasonic method and two-step electrodeposition. The degradation efficiency of TGC at the Pd-ZnS NPs/CF composite electrode was as high as 88.75 % within 30 min under neutral conditions, and the energy consumption was only 0.11 kWh/m³ . In addition, the overall Pd 2+ contribution was as low as 0.00005 M after the introduction of inexpensive ZnS. The Pd-ZnS NPs/CF composite electrode exhibited excellent electrocatalytic activity and degradation of antibiotics under different aqueous environmental conditions. Suitable for degradation of a wide range of antibiotics with excellent cyclic stability. The TGC degradation rate was stabilized above 85.40 % after 240 min of continuous operation in a continuous flow reactor, and the composite electrode prepared once can be used for a long time. It highlights the broad application prospects of the composite electrode in the field of water treatment technology and the significant advantages of high efficiency, low energy consumption and stable long-term operation. In this study, the mechanism of action of ·OH and H* with TGC is also deeply investigated, and the dynamics of TGC degradation intermediates are probed using two-dimensional correlation spectroscopy (2DCOS) and density-functional theory (DFT) calculations. Finally, the ecotoxicity of Pd-ZnS NPs/CF composite electrodes is investigated, which provides a simple and feasible idea for the preparation of efficiently degraded antibiotic cathodes.
研究领域:
环境科学
·
材料科学
·
生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）