ACS Biomaterials Science & Engineering
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2025
·
IF 5.5
Jiahao Xu,Dongzhe Zou,Enyu Dong,Xingyu Jiang,Shuo Xu,Yusheng Xiao,Wensheng Gong,Yini Xu,Zenan Zeng,Kefurong Deng,Yachao Li,Xianghui Xu
The rising incidence of bacterial infections poses a significant challenge to global public health. The development of safe and effective antibacterial treatment strategies is an urgent need in the field of biomedicine. In this work, we developed a smart nanoparticle–hydrogel platform to address bacterial infections in wounds. Rifampicin-loaded chitosan-functionalized nanoparticles (R-CNP) could break bacterial barriers and enhance antibiotic internalization. R-CNP reduced the minimum inhibitory concentration of rifampicin against Staphylococcus aureus and greatly enhanced the bactericidal effect of rifampicin. Furthermore, R-CNP was incorporated into thermosensitive hydrogels (HG) to construct HG(R-CNP) for enhanced antibiotic accumulation and wound protection. In the mouse model with a bacterial-infected wound, treatment with R-CNP reduced the bacterial content by 98.5% as compared to treatment with free rifampicin. Therefore, this smart nanoparticle–hydrogel platform constructed by FDA-approved or natural polymers, offers significant therapeutic efficacy on bacterial-infected wounds, showing great promise for clinical translation.
研究领域:
生物医学工程
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药剂学
·
微生物学
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DOI
PubMed
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-9
·
IF 5.228
Cui Min-Hua,Cui Dan,Lee Hyung-Sool,Liang Bin,Wang Ai-Jie,Cheng Hao-Yi
In this study, two modes of hybrid anaerobic digestion (AD) bioreactor with built-in BESs (electrodes installed in liquid phase (R1) and sludge phase (R2)) were tested for identifying the effect of electrodes position on azo dye wastewater treatment. Alizarin yellow R (AYR) was used as a model dye. Decolorization efficiency of R1 was 90.41 ± 6.20% at influent loading rate of 800 g-AYR/ m 3 ·d, which was 39% higher than that of R2. The contribution of bioelectrochemical reduction to AYR decolorization (16.23 ± 1.86% for R1 versus 22.24 ± 2.14% for R2) implied that although azo dye was mainly removed in sludge zone, BES further improved the effluent quality, especially for R1 where electrodes were installed in liquid phase. The microbial communities in the electrode biofilms (dominant by Enterobacter) and sludge (dominant by Enterococcus) were well distinguished in R1, but they were similar in R2. These results suggest that electrodes installed in liquid phase in the anaerobic hybrid system are more efficient than that in sludge phase for azo dye removal, which give great inspirations for the application of AD-BES hybrid process for various refractory wastewaters treatment.
研究领域:
环境工程
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生物技术
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废水处理
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DOI
PubMed
高影响因子
Journal of Energy Chemistry
·
2022
·
IF 13.599
Miao Wang,Zhenpeng Yao,Qianqian Li,Yongfeng Hu,Xiuping Yin,Aibing Chen,Xionggang Lu,Jiujun Zhang,Yufeng Zhao
Wadsley-Roth (W-R) structured oxides featured with wide channels represent one of the most promising material families showing compelling rate performance for lithium-ion batteries. Herein, we report an in-depth study on the fast and extensive intercalation chemistry of phosphorus stabilized W-R phase PNb 9 O 25 and its application in high energy and fast-charging devices. We explore the intercalation geometry of PNb 9 O 25 and identify two geometrical types of stable insertion sites with the total amount much higher than conventional intercalation-type electrodes. We reveal the ion transportation kinetics that the Li ions initially diffuse along the open type III channels and then penetrate to edge sites with low kinetic barriers. During the lithiation, no remarkable phase transition is detected with nearly intact host phosphorous niobium oxide backbone. Therefore, the oxide framework of PNb 9 O 25 keeps almost unchanged with all the fast diffusion channels and insertion cavities well-maintained upon cycling, which accomplishes the unconventional electrochemical performance of W-R structured electrodes.
研究领域:
材料科学
·
电化学
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DOI
高影响因子
Sustainable Chemistry and Pharmacy
·
2022
·
IF 5.464
Zhenwei Zhang,Xiaoshi Ma,Chuanjun Yue,Xuejiao Wei,Baoliang Liu,Xiaohui Chen
Hazardous waste alkaline batteries and waste fatty acids significantly threaten the environment. In this study, waste alkaline batteries were simply transferred to r-MnZn catalysts, which were then applied in the ketonization of fatty acids. The assessment results show that r-MnZn can efficiently convert n-octanoic acid to 8-pentadecanone. The conversion and yield are evaluated as 100% and 92.9%, respectively, which are much better than those of TiO 2 , ZrO 2 , CeO 2 and other metal oxides . The excellent catalytic efficiency is derived from the MnO 2 nanorods in r-MnZn materials which are characterized by XRD, TEM, and XPS. In addition, when using different kinds of fatty acids in ketonization, longer carbon chains require a higher activation energy, and the conversion of fatty acids was lower. Furthermore, the ketone products have a short carbon chain that induces side reactions, leading to a low yield. The above results indicate that r-MnZn is an efficient catalyst applied in the ketonization of fatty acids.
研究领域:
环境科学
·
催化化学
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DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Wenfei Zhou,Mo Yang,Zhi Zhao,Shaopeng Li,Zhiqiang Cheng,Jinsong Zhu
Surface-grafted polymer brushes have been long reported as a desired matrix for biosensing applications, owing to enhanced immobilization capacity and anti-fouling properties. However, simultaneous optimizing binding signal and minimizing non-specific adsorption using polymer matrix is still a challenge, due to the lack of systematic structural and composition optimization at molecular level. In this research, a series of block copolymer brushes with controlled hierarchical architectures, poly [oligo (ethylene glycol) methacrylate- b -glycidyl methacrylate] (POEMGA- b -PGMA) were prepared by two-stage surface-initiated polymerization. Surface plasmon resonance imaging (SPRi), as a real-time and label-free biosensing technology, was used as means to evaluate their detecting performances of bio-interactions. In contrast to one-layer poly (glycidyl methacrylate) (PGMA), 4.7-fold enhancement of signal-to-background ( S / B ) was achieved in bi-layer POEGMA- b -PGMA surface, by using rabbit IgG (R-IgG, in 10% serum) and a microarray of goat-anti-rabbit IgG (G-R-IgG) as the system of study. Interestingly, the binding signals and anti-fouling performances of the polymer brushes could be enhanced simultaneously by increasing the length of the monomer in the primary layer. Theoretically, it mainly owed to the formation of a densely packing protective primary layer against non-specific physical adsorption and a spatially optimized secondary layer of minimized steric hindrance that allowed optimum binding of analytes.
研究领域:
材料科学
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生物传感器
·
高分子化学
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分析化学
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DOI
高影响因子
SPECTROSCOPY LETTERS
·
2025
·
IF 1.6
Ju Wei,Bingyu Luo,Jiao Fu,Shunli Zhou,E. Li,Xiaodong Su,Xiaotong Gao,Zhenfu Jia,Xiaojiang Li
To overcome the interference caused by the inner filter effect, this study optimally selected red fluorescence carbon dots as the signal source and constructed a novel dual-mode Ag@Red-CDs nanoprobe for the highly selective detection of Fe³+ using fluorescence and colorimetry. This probe is based on a unique reaction between Fe3+ and silver nanoparticles (AgNPs) that weakens the static quenching effect of AgNPs on R-CDs; at the same time, the depletion of AgNPs induces a change in the absorbance of the system. Under optimal conditions, the probe exhibited a linear fluorescence response in the Fe³+ concentration range of 0.10–25.00 μg/mL and a linear colorimetric response of 0.18–8.00 μg/mL. The detection limits for fluorescence and colorimetry were 0.31 μg/mL and 0.56 μg/mL, respectively. In spiked real samples, the fluorescence method achieved Fe³+ recovery rates of 99.10–102.34%, while the colorimetric method showed recovery rates of 99.83–101.42%. Overall, this research significantly broadened the potential applications of R-CDs-based materials for the quantitative detection of heavy metal ions in water bodies.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2024
·
IF 4.8
Danilo de Souza Costa,Rayssa A. Cajas,Carla Monteiro Leal,Lara S. Aleixo de Carvalho,Lara Cruz de Souza,Lucas Fukui-Silva,Josué de Moraes,Ademar A. Da Silva Filho
Ethnopharmacological relevance Acmella oleracea (L.) R. K. Jansen, commonly referred to as “Jambu”, is a valuable medicinal plant native to the Amazon regions. Inflorescences of A. oleracea is utilized as local anesthetic properties and for its insecticidal, antiparasitic, and anthelmintic attributes. Aims of this study This study aimed to evaluate the in vitro and in vivo antiparasitic properties of A. oleracea extract and spilanthol (SPL) against Schistosoma mansoni . Materials and methods The ethanolic extract of A. oleracea inflorescences (AoE) was prepared, analyzed by HPLC-DAD, and characterized by UHPLC-HRMS/MS. SPL was isolated from AoE by chromatographic fractionation. The antischistosomal properties of AoE and SPL were evaluated in vitro against adult schistosomes and in preclinical assays using murine models of patent S. mansoni infection. Results Through UHPLC-HRMS/MS analysis, 14 alkamides were annotated in AoE. HPLC-DAD analysis of AoE revealed a peak with a substantial relative area of ∼85%, which was isolated and identified as SPL. AoE and SPL caused mortality of adult schistosomes ex vivo , showing EC 50 values of 32.6 μg/mL and 27.8 μM, respectively, without affected Vero cells or Caenorhabditis elegans . In preclinical studies, the oral administration (400 mg/kg) of AoE and SPL resulted in significant reductions in worm burden of 28% and ∼42%, respectively. Additionally, SPL exhibits remarkable effects on oogram, decreasing egg burden and the number of immature eggs by over 80%, while also promoting significant reductions in hepato- and splenomegaly. Conclusions The research underscores the antischistosomal activity of A. oleracea highlighting the potential of SPL as a lead for the development of new schistosomicidal drugs and encouraging further studies to validate the traditional anthelmintic use of A. oleracea inflorescences.
研究领域:
药理学
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寄生虫学
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药物开发
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Huajian Xu,Zhongtao Hu,Zongwei Hao,Changyue Deng,Xiaowen Pi,Binjia Zhang
The effects of amino acids with different R-groups, namely glutamic acid (GLU), glutamine (GLN), and theanine (THE), on the pasting and structure properties of corn starch (CS) were investigated. During gelatinization, GLU decreased viscosity of CS. GLN and THE increased pasting enthalpy of CS from 7.62 to 8.72 and 9.50 J/g, respectively. All starch-amino acid systems had higher storage and loss modulus. Amino acids could adhere to starch granule surface. After co-gelatinization, GLU increased short-range order degree of starch, while GLN and THE decreased. Both infrared spectroscopy and quantum computation showed that non-covalent interaction occurred between amino acids and starch. Molecular dynamics revealed that the interaction between GLU and starch weakened hydrogen bonding between starch and water, promoting cross-linking between starch chains. GLN and THE changed dihedral angle torsion of glycoside bonds, and mainly interacted with starch by electrostatic interaction (50.06 kJ/mol) and van der Waals force (50.30 kJ/mol), respectively.
研究领域:
食品科学
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化学
·
材料科学
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DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2017
·Vol. 24
·期 5
·1-9
·
IF 1.615
Xue Sheng,Yang Haicun,Ma Wenzhong,Gong Fanghong,Tao Guoliang,Liu Chunlin,Pan Ji
Poly(methyl methacrylate) brushes grafted from attapulgite nanoparticles (ATP@PMMA) were prepared by R-Supported reversible addition-fragmentation chain transfer polymerization (RAFT). ATP was firstly activated, and allowed to react with γ-aminopropyltriethoxysilane (APTES), 4,4′-azobis(4-cyanovaleric acid) (ACVA) and bis(thiobenzoyl) disulfide stepwise to afford 4-cyanopentanoic acid dithiobenzoate functionalized ATP (ATP-CPADB). Then, RAFT polymerizations of MMA mediated by the different systems were conducted and compared systemically. The grafting process was verified by FTIR, XRD, XPS, TGA and TEM data. Kinetic behavior indicated that the anchored CPADB is more effective before diffusing into the bulk phase. The grafting ratio ( G
r ), while M
n of grafted and free polymer increased linearly with increasing monomer conversion in presence of free CPADB, giving the hybrid particles with more homogeneous distribution of grafted polymer layer.
研究领域:
材料科学
·
高分子化学
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DOI
高影响因子
Bioresources and Bioprocessing
·
2019
·Vol. 6
·期 1
·1-12
·
IF 0
Zhang Can,Song Wei,Liu Jia,Chen Xiulai,Liu Liming
( R )- or ( S )-2-Hydroxy-4-(methylthio)butanoic acid (HMTBA) is used as a poultry nutritional supplement and to treat renal failure disease. Herein, we report an artificially designed biocatalytic cascade process, which uses l -methionine to synthesize ( R )- or ( S )-HMTBA. This biocatalysis cascade comprises a basic module and two different extender modules and operates in a modular assembly manner. The basic module responsible for the transformation of l -methionine to α-keto-γ-methylthiobutyric acid (KMTB) is comprised of the l -amino acid deaminase. Two different extender modules responsible for the transformation of KMTB to ( R )- or ( S )-HMTBA are comprised of the R/S-specific lactate dehydrogenase in combination with the formate dehydrogenase, respectively. Engineered Escherichia coli catalysts, one containing the basic module, the other containing the one of two different extender modules, produced 97.6 g L −1 ( R )-HMTBA and 96.4 g L −1 ( S )-HMTBA with a yield of 96.9% and 95.8% at the large scale (1 L) using a two-stage strategy in one pot, respectively. Therefore, this biocatalytic process lays the foundation for the industrial-scale conversion of low-cost l -amino acids to corresponding high-value enantiopure chiral 2-hydroxy acids.
研究领域:
生物技术
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酶工程
·
代谢工程
·
合成生物学
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DOI
Journal of Ginseng Research
·
2022
·
IF 5.735
Hong-Yan Guo,Yue Xing,Yu-Qiao Sun,Can Liu,Qian Xu,Fan-Fan Shang,Run-Hui Zhang,Xue-Jun Jin,Fener Chen,Jung Joon Lee,Dongzhou Kang,Qing-Kun Shen,Zhe-Shan Quan
Background Ginseng possesses antitumor effects, and ginsenosides are considered to be one of its main active chemical components. Ginsenosides can further be hydrolyzed to generate secondary saponins, and 20( R )-panaxotriol is an important sapogenin of ginsenosides. We aimed to synthesize a new ginsengenin derivative from 20( R )-panaxotriol and investigate its antitumor activity in vivo and in vitro . Methods Here, 20( R )-panaxotriol was selected as a precursor and was modified into its derivatives. The new products were characterized by 1 H-NMR, 13 C-NMR and HR-MS and evaluated by molecular docking, MTT, luciferase reporter assay, western blotting, immunofluorescent staining, colony formation assay, EdU labeling and immunofluorescence, apoptosis assay, cells migration assay, transwell assay and in vivo antitumor activity assay. Results The derivative with the best antitumor activity was identified as 6,12-dihydroxy-4,4,8,10,14-pentamethyl-17-(2,6,6-trimethyltetrahydro-2 H -pyran-2-yl)hexadecahydro-1 H -cyclopenta[a]phenanthren-3-yl( tert -butoxycarbonyl)glycinate ( A11 ). The focus of this research was on the antitumor activity of the derivatives. The efficacy of the derivative A11 (IC 50 < 0.3 μM) was more than 100 times higher than that of 20( R )- panaxotriol (IC 50 > 30 μM). In addition, A11 inhibited the protein expression and nuclear accumulation of the hypoxia-inducible factor HIF-1α in HeLa cells under hypoxic conditions in a dose-dependent manner. Moreover, A11 dose-dependently inhibited the proliferation, migration, and invasion of HeLa cells, while promoting their apoptosis. Notably, the inhibition by A11 was more significant than that by 20( R )-panaxotriol ( p < 0.01) in vivo . Conclusion To our knowledge, this is the first study to report the production of derivative A11 from 20( R )-panaxotriol and its superior antitumor activity compared to its precursor. Moreover, derivative A11 can be used to further study and develop novel antitumor drugs.
研究领域:
药理学
·
肿瘤学
·
有机化学
·
生物化学
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DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2026
·
IF 5.4
Yanqing Wu,Xilin Qiao,Shuaiyu Chen,Yalan Lu,Bingyan Mao,Mengdie Zhu,Xinzhi Chen,Nipi Chen,Zhishan Ding,Bo Jin
Introduction MicroRNA-29b-3p (miR-29b-3p) plays a critical role in regulating endothelial apoptosis and autophagy. We previously demonstrated that protocatechualdehyde (PCA), a water-soluble phenolic acid derived from Salvia miltiorrhiza Bge., protects human umbilical vein endothelial cells (HUVECs) against oxygen-glucose deprivation/reoxygenation (OGD/R)-induced injury. However, whether miR-29b-3p mediates this protective effect remains unclear. Methods Using the OGD/R in vitro model of ischemia-reperfusion injury, we investigated the molecular mechanisms underlying ischemic damage at the cellular level. RT-qPCR was used to measure miR-29b-3p expression, Western blotting to assess autophagy- and apoptosis-related proteins, and MDC staining and flow cytometry to evaluate autophagy and apoptosis, respectively. Results The results showed that miR-29b-3p was downregulated in OGD/R-treated HUVECs, an effect that was reversed by PCA and the ROS inhibitor N-acetylcysteine (NAC). The miR-29b-3p mimic enhanced the promoting effects of PCA and NAC on miR-29b-3p expression, whereas miR-29b-3p inhibitor reversed these effects. Similarly, PCA-induced autophagy and inhibition of apoptosis were enhanced by the miR-29b-3p mimic and reversed by the inhibitor. To determine whether SIRT1 acts downstream of miR-29b-3p, we used EX527 (a SIRT1 inhibitor) and found that it attenuated the PCA-mediated upregulation of miR-29b-3p. The miR-29b-3p mimic enhanced PCA-induced SIRT1 activity and protein expression, while the inhibitor had the opposite effect. Additional experiments revealed that NAC suppressed ROS by upregulating miR-29b-3p, leading to increased SIRT1 expression and reduced apoptosis. Disscussion Collectively, these findings demonstrate that PCA ameliorates OGD/R-induced endothelial injury by promoting autophagy and inhibiting apoptosis via the ROS/miR-29b-3p/SIRT1 regulatory axis.
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PubMed
高影响因子
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