JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Yu Mao,Shengjie Gao,Lili Yao,Lu Wang,Hao Qu,Yuen Wu,Ying Chen,Lei Zheng
As a high biologically toxic heavy metal ion , Cr(VI) will cause environmental pollution and endanger human health. Therefore, the development of fast, simple and visible detection methods for Cr(VI) is extremely important to control its harm. Toward this end, we report the establishment of a colorimetric sensing method for Cr(VI) based on single-atom nanozymes for enhanced detection performance. Firstly, we prepared SA-Fe/NG as peroxidase mimetic by anchoring Fe single-atom onto a single-layer of two-dimensional nitrogen-doped graphene. The SA-Fe/NG showed superiorly high oxidation catalytic activity due to its 100% atomic utilization and existing Fe-N-C structure. Furthermore, with 3,3′,5,5′-tetramethylbenzidine (TMB) as a colorimetric sensing probe, and 8-hydroxyquinoline (8-HQ) as an inhibitor for the oxidation of TMB, the detection of Cr(VI) was realized through specific interaction between Cr(VI) and 8-HQ, which led to the recovery of oxTMB in blue color. Our established method showed superior sensitivity with a detection limit of 3 nM and a linear range of 30 nM to 3 μM. It also exhibited high selectivity for a series of metal cations, and has been successfully applied to the detection of Cr(VI) in tap water and tuna samples.
研究领域:
环境科学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2021
·Vol. 14
·期 11
·2308-2316
·
IF 3.366
Yuan Wei,Lu Lixia,Lu Yichen,Xiong Xiong,Li Yi,Cui Xiaowen,Liu Yuanjian,Xiong Xiaohui
Ochratoxin A (OTA) is considered as one of the most hazardous mycotoxins commonly occurring in grains. Thus, the monitoring of OTA is extremely significant. Herein, a synergistic strategy to detect OTA in grains based on the combination of DNA tetrahedron-structured aptamer (DTA) and hybridization chain reaction (HCR) was proposed. Briefly, DTA was assembled on the gold electrode. In the presence of OTA, the trigger DNA on the top of the DTA was exposed to unfold the biotin-modified hairpin structure DNA to HCR. It could conjugate plentiful streptavidin-labeled horseradish peroxidase (SA-HRP) to form polyaniline (PANI), which was regarded as electrochemical active molecule to detect OTA. In this way, the synergistic effect of DTA and HCR not only can provide stable probe structure to improve the recognition rate of OTA, but also can supply a large number of templates to synthesize PANI under mild conditions. As a result, the biosensor has a good analytical performance for OTA in a linear range of 0.05 to 50 ng mL −1 , with a detection limit of 0.017 ng mL −1 . The accuracy of sensor is similar with high-performance liquid chromatography (HPLC) analysis.
研究领域:
分析化学
·
生物传感器
·
毒理学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2023
·
IF 10
Qianqian Wu,Lu Yang,Ling Zou,Wang Yang,Qingshan Liu,Anwei Zhang,Jiang Cao,Guangyou Shi,Jian He,Xiaochao Yang
The over production of reactive oxygen species (ROS) plays a critical role in the progression of chronic kidney disease (CKD). Organic ROS scavengers currently used for CKD treatment do not satisfy low dosage and high efficiency requirements. Ceria nanomaterials featured with renewable ROS scavenging activity are potential candidates for CKD treatment. Herein, a method for the synthesis of ceria nanoclusters (NCs) featured with small size of ≈1.2 nm is reported. The synthesized NCs are modified by three hydrophilic ligands with different molecular weights, including succinic acid (SA), polyethylene glycol diacid 600 (PEG600), and polyethylene glycol diacid 2000 (PEG2000). The surface modified NCs exhibit excellent ROS scavenging activity due to the high Ce 3+ /Ce 4+ ratio in their crystal structures. Compared with bigger-sized ceria nanoparticles (NPs) (≈45 nm), NCs demonstrate smoother blood concentration-time curve, lower organ accumulation, and faster metabolic rate superiorities. The administration of NCs to CKD mice, especially PEG600 and PEG2000 modified NCs, can effectively inhibit oxidative stress, inflammation, renal fibrosis, and apoptosis in their kidneys. Due to these benefits, the constructed NCs can ameliorate the progression of CKD. These findings suggest that NCs is a potential redox nanomedicine for future clinical treatment of CKD.
研究领域:
纳米技术
·
药学
·
肾病学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DESALINATION
·
2024
·
IF 8.3
Linzhe Xu,Yingbo Chen,Yuanyuan Zhang,Meng Wang,Siyu Wei,Xinyu Zhao
Uranium, as a radioactive element, is the source of utilizable energy for mankind in the future. Large quantities of uranium resources are contained in seawater, so uranium extraction from seawater is undoubtedly the most feasible method. However, the presence of competitive ions such as vanadium hinders the efficient extraction of uranium, which is currently a challenge that needs to be solved. Here, silane coupling agent (KH550) was grafted onto two-dimensional metal carbide (MXene). Subsequently, salicylaldoxime (SA) was deposited onto MXene particles using self-polymerization of dopamine (DA) to prepare PDA/SA deposited MXene composite grafted with KH550 (PDA/SA/MXene-KH550). The adsorbent exhibited excellent selectivity for uranium ions with a uranium/vanadium selectivity ratio of 4.8. In antibacterial experiments, the PDA/SA/MXene-KH550 composite showed excellent antibacterial performance against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ), demonstrating the ability to adapt to complex marine microbial contamination. In addition, the maximum experimental uranium adsorption capacity of the composite was 332.3 mg/g, following the pseudo-second-order kinetic model and the Langmuir isotherm model. After five adsorption-desorption cycles, the elution efficiency of PDA/SA/MXene-KH550 for uranium can still reach 74.7 %, which demonstrated that the adsorbent had superior stability and regeneration ability, providing the possibility for actual enrichment of uranium.
研究领域:
材料科学
·
环境科学
·
核工程
·
化学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Mingxing Li,Xuwei Shang,Haiya Lou,Zixu Wang,Shanshan Xiang,Yihe Qiu,Fuqiang Hu,Fangying Yu,Hong Yuan
Recently, nanotechnology-based drug delivery platforms in treating pulmonary arterial hypertension (PAH) have gradually emerged. However, large mechanical stress and shear stress in blood vessels greatly affect the retention of nanopreparative materials at lesion sites, severely limiting nanotechnology-based drug delivery. Herein, a stimuli-responsive nanocraft is rationally designed by actively anchoring E-selectin overexpressed on pulmonary arterial endothelial cells (PAECs), under hypoxic conditions, allowing effective accumulation and retention of the drug at the lesion site. Briefly, a nitrobenzene group is incorporated into the framework of a nanocarrier, and then it is simultaneously linked with chitosan. Additionally, the surface of the nanocarrier with sialic acid (SA) and encapsulated the clinically used drug ambrisentan (Am), which enables the anchoring of E-selectin and subsequent drug delivery is modifed. This system facilitates intercellular transport to pulmonary artery smooth muscle cells (PASMCs) when targeting PAECs and specifically responds to a reductive hypoxic microenvironment with elevated nitroreductase in PASMCs. Moreover, compared with free Am, nanoencapsulation and SA-PEG 2000 -NH 2 prolong the blood circulation time, achieving better therapeutic outcomes in preventing vascular remodeling and reversing systolic dysfunction. The originality and contribution of this work reveal the promising value of this pulmonary arterial anchoring stimuli-responsive nanocraft as a novel therapeutic strategy for satisfactory PAH treatment.
研究领域:
纳米技术
·
药学
·
心血管医学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2024
·
IF 3.7
Yidan Zhang,Hui Lian,Linhui Yang,Lingling Tong,Yang Wu,Suxing Jin,Dongsheng Guo
Ophiocordyceps gracilis is a type of fungus commonly utilized in both health food and Chinese medicine. Herein, we prepared antioxidant hydrolysate from O. gracilis , and isolated and purified the prepared hydrolysate to obtain O. gracilis peptides-4-3-1 (OGP-4-3-1) grade with the highest scavenging activity on DPPH, ABTS and hydroxyl radicals. Three new peptides with high antioxidant capacity were identified as LFNHF, PLDRHPF and YPFLRPL, respectively. The three peptides can reduce the proportion of senescent cells conveying positive expression of senescence-associated-β-galactosidase (SA-β-Gal) and increase survival pace of H 2 O 2 -induced human hepatocellular carcinomas (HepG2 cells), and the protective effects on cellular against senescence might be related to their increase in the activities of antioxidant enzymes, as well as the decrease in the concentration of malondialdehyde (MDA) and reactive oxygen species (ROS). The molecular docking results indicated that they could form durable hydrophobic and hydrogen bonds with critical amino acid residues of the Kelch-like ECH-associated protein 1 (Keap1), which could potentially modulate pathway of nuclear factor erythroid 2-related factor 2 (Nrf2) in conjunction with Keap1. In conclusion, the current research offers a theoretical foundation for the further development of the anti-aging effect of OGP, which has a broad application prospect in the fields of functional food and cosmetics.
研究领域:
生物化学
·
药理学
·
细胞生物学
·
食品科学
·
化妆品学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Baozhang Shi,Tong Zhu,Yang Luo,Xiang Zhang,Jie Yao,Xu Cao,Yingchun Zhu,Hongyue Miao,Liangliang Li,Qin Song,Hua Zhang,Liping Xu
Engineered matrices with aligned microarchitectures are pivotal in regulating the fibroblast-to-myofibroblast transition, a critical process for wound healing and scar reduction. However, developing a three-dimensional (3D) aligned matrix capable of effectively controlling this transition remains challenging. Herein, we developed a cell-adaptive hydrogel with highly oriented microporous structures, fabricated through bioprinting of thermo/ion/photo-crosslinked gelatin methacrylate/sodium alginate (GelMA/SA) incorporating shear-oriented polyethylene oxide (PEO) filler. The synergistic interactions among GelMA, PEO, and SA yield a homogeneous mixture conducive to the printing of biomimetic 3D constructs with anisotropic micropores. These anisotropic micropores, along with the biochemical cues provided by the GelMA/PEO/SA scaffolds, enhance the oriented spreading and organization of fibroblasts. The resultant spread and aligned cellular morphologies promote the transition of fibroblasts into myofibroblasts. By co-culturing human keratinocytes on the engineered dermal layer, we successfully created a bilayer skin construct, wherein the keratinocytes established tight junctions accompanied by elevated expression of cytokeratin-14, while the fibroblasts displayed a highly spread morphology with increased fibronectin expression. The printed hydrogels facilitated accelerated full-thickness wound closure by establishing a bioactive microenvironment that mitigated inflammation and stimulated angiogenesis, myofibroblast transition, and extracellular matrix remodeling. This anisotropic hydrogel demonstrates substantial promise for applications in skin tissue engineering.
研究领域:
组织工程
·
生物医学工程
·
伤口愈合
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Zhiwei Zhu,Hui Liang,Da-Wen Sun
Undesired ice/frost formation and accretion often occur on food freezing facility surfaces, lowering freezing efficiency. In the current study, two slippery liquid-infused porous surfaces (SLIPS) were fabricated by spraying hexadecyltrimethoxysilane (HDTMS) and stearic acid (SA)-modified SiO2 nanoparticles (NPs) suspensions, separately onto aluminum (Al) substrates coated with epoxy resin to obtain two superhydrophobic surfaces (SHS), and then infusing food-safe silicone and camellia seed oils into the SHS, respectively, achieving anti-frosting/icing performance. In comparison with bare Al, SLIPS not only exhibited excellent frost resistance and defrost properties but also showed ice adhesion strength much lower than that of SHS. In addition, pork and potato were frozen on SLIPS, showing an extremely low adhesion strength of <10 kPa, and after 10 icing/deicing cycles, the final ice adhesion strength of 29.07 kPa was still much lower than that of SHS (112.13 kPa). Therefore, the SLIPS showed great potential for developing into robust anti-icing/frosting materials for the freezing industry.
研究领域:
材料科学
·
表面工程
·
食品技术
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Xinli Wang,Hongyan Liu,Shaoqiang Zhang,Zhongcui Jing,Changkai Zhang,Songxia Yan,Hong Zhou,Haiyan Wang
Background Recently, various techniques have been developed to accurately and sensitively detect tumor biomarkers for the early diagnosis and effective therapy of cancer. The electrochemiluminescence (ECL) method holding outstanding features including high sensitivity, ease of operation, and spatiotemporal controllability exhibited great potential for DNA/RNA detection, immunoassay, cancer cell detection, and environmental analysis. However, a glaring problem of ECL approaches is that the layer-by-layer modification on the electrode leads to poor stability and sensitivity of the sensors. Therefore, new simple and efficient methods for electrode modification which can effectively improve the ECL signal have attracted more and more research interests. Results Based on the dual amplification strategy of target-induced CHA and nanocomposite probes leading to self-generated co-reactant (H 2 O 2 ), we proposed a highly sensitive miRNA-ECL detection system. The introduction of the target miRNA-21 triggers the CHA cycle amplification of DNA1 and biotin-modified DNA2, releasing the target miRNA-21 sequence for the target cycle reaction. After the reaction, the newly introduced DNA2 was combined with Au NPs modified with SA and Glucose oxidase (GOD). In the presence of oxygen, glucose was decomposed by GOD to produce H 2 O 2 , and then H 2 O 2 was immediately catalyzed by the Hemin/G-quadruplex at the double-stranded end of the CHA product to produce a large amount of O 2 −• . As a co-reactant of luminol, the ECL signal was significantly enhanced, thereby achieving highly sensitive detection of miRNA-21 content and obtaining a low detection limit of 0.65 fM. The high specificity of the ECL biosensor was also proved by base mismatch. Significance Compared with other current detection methods, this sensor can achieve quantitative analysis of other target analytes by flexibly changing the probe DNA sequence, and provide a new feasible solution for the detection of tumor-associated markers. Benefiting from the improved sensitivity and selectivity, the proposed biosensing platform is expected to provide a new strategy for biomarkers analysis and outstanding prospect for further clinical application.
研究领域:
生物医学工程
·
分析化学
·
分子生物学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Shuchun Gan,Shihang Bai,Cheng Chen,Yongliang Zou,Yingjuan Sun,Jianhao Zhao,Jianhua Rong
Ionic conductive hydrogels with both high-performance in conductivity and mechanical properties have received increasing attention due to their unique potential in artificial soft electronics. Here, a dual physically cross-linked double network (DN) hydrogel with high ionic conductivity and tensile strength was fabricated by a facile approach. Hydroxypropyl cellulose (HPC) biopolymer fibers were embedded in a poly (vinyl alcohol)‑sodium alginate (PVA/SA) hydrogel, and then the prestretched PVA-HPC/SA composite hydrogel was immersed in a CaCl 2 solution to prepare PVA-HPC T /SA-Ca DN hydrogels. The obtained composite hydrogel has an excellent tensile strength up to 1.4 MPa. Importantly, the synergistic effect of hydroxypropyl cellulose (HPC) and prestretching reduces the migration resistance of ions in the hydrogel, and the conductivity reaches 3.49 S/ m. In addition, these composite hydrogels are noncytotoxic, and they have a low friction coefficient and an excellent wear resistance. Therefore, PVA-HPC T /SA-Ca DN hydrogels have potential applications in nerve replacement materials and biosensors.
研究领域:
材料科学
·
生物医学工程
·
纳米技术
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Mingxin Qiao,Zhengyi Xu,Xibo Pei,Yanhua Liu,Jian Wang,Junyu Chen,Zhou Zhu,Qianbing Wan
Hyperlipidemia negatively affects osteogenesis, thereby impairing bone healing. However, few biomaterials have been developed for bone tissue engineering to solve this problem. Herein, the preparation of specific biomaterials that can promote osteogenesis and suppress adipogenesis holds great promise for reconstructive bone defect treatment. In this study, we propose the construction of a nano simvastatin-laden zeolitic imidazolate framework-8 (nano [email protected] ) modified injectable high-intensity biohydrogel, composed of poly (ethylene glycol) diacrylate (PEGDA) and sodium alginate (SA) to form nano [email protected] /PEGDA/SA (defined as nSZPS), which can stimulate osteogenic differentiation and inhibit adipogenic differentiation. The characterization and drug loading efficiency of the nanoparticles were examined to confirm the successful synthesis of nano [email protected] Within the PEGDA/SA delivery platform, nSZPS showed excellent injectability and mechanical strength through rheological and mechanical tests. Moreover, owing to the sustained release of SIM and bioactive Zn ions, nSZPS not only possessed excellent biocompatibility but also significantly enhanced the osteogenic differentiation ability and inhibited the adipogenic efficacy of BMSCs in vitro. Furthermore, nSZPS showed excellent osseointegration and lipid-lowering abilities in healing bone defects of hyperlipidemic rats by decreasing PPARγ expression and increasing RUNX2 expression in vivo. In addition, the bidirectional regulatory mechanisms of nSZPS in bone defects may be closely related to the mutual regulation of the PPARγ and Wnt/β-CATENIN pathways. In summary, this nano [email protected] modified injectable high-intensity biohydrogel might be a promising strategy for complex bone regeneration, especially in hyperlipidemic microenvironments.
研究领域:
生物材料
·
组织工程
·
骨再生
·
药物递送系统
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Shiyao Guo,Mingyue Zhang,Ke Zhang,Xiaotong Lu,Peiran Chen,Bangliu Yang,Xueqian Wang,Yuhong Zhuo,Dongqin Yu,Chao Qi,Kaiyong Cai
Osteoporosis compromises bone repair by generating a dysregulated microenvironment marked by excessive accumulation of reactive oxygen species (ROS) and a reduced pH. This pathological state leads to mitochondrial dysfunction, impaired osteogenic differentiation, and excessive activation of osteoclasts. In this study, a ROS-responsive titanium implant coating composed of calcium carbonate-mineralized nicotinamide mononucleotide (NMN) nanoparticles, lipoic acid-modified gelatin (LAMG), and sodium alginate (SA) is developed, designed to enhance osseointegration through dual synergistic mechanisms. First, the LAMG hydrogel effectively scavenges excess ROS, while calcium carbonate neutralizes H + ions and restores physiological pH levels, thereby reducing acid-induced bone resorption and inhibiting osteoclast activity. Second, ROS and H + ions-triggered release of NMN enhances the intracellular NAD⁺/NADH ratio in bone marrow mesenchymal stem cells, activates the SIRT1 signaling pathway, restores mitochondrial function, and promotes osteogenic differentiation. By concurrently remodeling the pathological bone microenvironment and repairing mitochondrial dysfunction, this multifunctional coating suppresses osteoclast-mediated bone resorption while facilitating osteoblast formation, thereby enhancing peri-implant osseointegration under osteoporotic conditions. This strategy not only presents a promising therapeutic approach for functionalizing titanium implants but also offers a potentially translatable platform for treating osteoporotic bone defects.
研究领域:
生物材料
·
组织工程
·
骨科
·
再生医学
·
纳米医学
·
骨骼生物学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）