Advanced Healthcare Materials
·
2021
·Vol. 10
·期 20
·2100985
·
IF 9.933
Yuanyuan Jia,Peiliang Shen,Tao Yan,Weijia Zhou,Jia Sun,Xin Han
Robust isolation of cancer stem cells (CSCs) in a high-throughput, label-free manner is critical for understanding tumor heterogeneity and developing therapeutic strategies targeting CSCs. Cell-mechanics-based microfluidic sorting systems provide efficient and specific platforms for investigation of stem cell-like characteristics on the basis of cell deformability and cell-substrate adhesion properties. In the present study, a microfluidic tandem mechanical sorting system is developed to enrich CSCs with high flexibility and low adhesive capacity. In the integrated microfluidic system, cancer cells are driven by hydrodynamic forces to flow continuously through two featured devices, which are functionalized with sequentially variable microbarriers and surface-coated fluid mixing microchannels, respectively. Collected deformable and low-adhesive cancer cells exhibit enhanced stem cell-like properties with higher stemness and metastasis capacity both in vitro and in vivo, compared with each single device separation. Using these devices, bioactive natural compound screening targeting CSCs is performed and a potent therapeutic compound isoliquiritigenin from licorice is identified to inhibit the lung cancer stem cell phenotype. Taken together, this microfluidic tandem mechanical sorting system can facilitate drug screening targeting CSCs and the analysis of signals regulating CSC function in drug resistance.
研究领域:
生物医学工程
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癌症生物学
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药理学
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细胞生物学
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药物发现
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DOI
PubMed
高影响因子
Oncotarget
·
2016
·
IF 5.008
Rui Yang,Li Y. An,Qin F. Miao,Feng M. Li,Yong Han,Hui X. Wang,Dang P. Liu,Rong Chen,Sha Q. Tang
Aim To investigate the use of thermosensitive magnetoliposomes (TMs) loaded with magnetic iron oxide (Fe 3 O 4 ) and the anti-cancer stem cell marker CD90 (CD90@TMs) to target and kill CD90 + liver cancer stem cells (LCSCs). Methods The hepatocellular carcinoma cell line Huh7 was used to separate CD90 + LCSCs by magnetic-activated cell sorting. CD90@TMs was characterized and their ability to target CD90 + LCSCs was determined. Experiments were used to investigate whether CD90@TMs combined with magnetic hyperthermia could effectively eliminate CD90 + LCSCs. Results The present study demonstrated that CD90 + LCSCs with stem cells properties were successfully isolated. We also successfully prepared CD90@TMs that was almost spherical and uniform with an average diameter of 130±4.6 nm and determined that magnetic iron oxide could be incorporated and retained a superparamagnetic response. CD90@TMs showed good targeting and increased inhibition of CD90 + LCSCs in vitro and in vivo compared to TMs. Conclusion CD90@TMs can be used for controlled and targeted delivery of anticancer drugs, which may offer a promising alternative for HCC therapy.
研究领域:
肿瘤学
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细胞生物学
·
纳米医学
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DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
·
2021
·Vol. 109
·期 12
·2227-2236
·
IF 3.368
Shuxian Shen,Xuzhao He,Xiaoyi Chen,Lingqing Dong,Kui Cheng,Wenjian Weng
Electrical stimulation has been proved to be critical to regulate cell behavior. But, cell behavior is also susceptible to multiple parameters of the adverse interferences such as surface current, electrochemical reaction products, and non-uniform compositions, which often occur during direct electric stimulation. To effectively prevent the adverse interferences, a novel piezoelectric poly(vinylidene fluoride-trfluoroethylene)(P(VDF-TrFE)) layer was designed to coat onto the indium tin oxide (ITO) planar microelectrode. We found the electrical stimulation was able to regulate the osteogenic differentiation of mesenchymal stem cells (MSCs) through calcium-mediated PKC signaling pathway. Meanwhile, the surface charge of the designed P(VDF-TrFE) coating layer could be easily controlled by the pre-polarization process, which was demonstrated to trigger integrin-mediated FAK signaling pathway, finally up-regulating the osteogenic differentiation of MSCs. Strikingly, the crosstalk in the downstream of the two signaling cascades further strengthened the ERK pathway activation for osteogenic differentiation of MSCs. This P(VDF-TrFE) layer coated electrical stimulation microelectrodes therefore provide a distinct strategy to manipulate multiple-elements of biomaterial surface to regulate stem cell fate commitment.
研究领域:
生物医学工程
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细胞生物学
·
材料科学
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DOI
PubMed
高影响因子
ACS Sensors
·
2026
·
IF 10.9
Hai Shi,Lu Liu,Ying Shen,Yunlong Xiao,Renwu Mu,Suzhen Zhai,Bin Zhang,Xiaoyan Yang,Chunlin Zhang
Leukemia stem cell (LSC) phenotyping offers significant potential to enhance minimal residual disease (MRD) monitoring in acute myeloid leukemia (AML), improving therapeutic evaluation and relapse prediction. However, current approaches lack the sufficient sensitivity and specificity to reliably detect rare chemotherapy-resistant LSCs (crLSCs) or identify stem-like phenotypic states, limiting clinical translation. Here, we develop DISCERN (Dual-Aptamer-Initiated Sensing Circuit via Engineered Nanozyme), a colorimetric platform for ultrasensitive LSC phenotyping. DISCERN employs a dual-aptamer system targeting colocalized surface markers (CD33 and CD123) for high-specificity recognition. Its exceptional sensitivity (limit of detection <10 cells/mL) is achieved by a localized catalytic cascade: target-binding initiates on-site rolling circle amplification (RCA), which in turn templates the assembly of PCN-222(Fe) nanozymes that generate amplified colorimetric signals. We demonstrate that DISCERN can track phenotypic plasticity in leukemia cells and identify stem-like subsets in leukemia xenograft models. This cost-effective and robust platform provides a promising tool for AML risk stratification, relapse prediction, and precision therapy.
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DOI
PubMed
高影响因子
Stem Cell Reviews and Reports
·
2026
·
IF 4.2
Yue Wei,Yang Yue,Miao Yan,Li Jinqi,Li Shanshan,Yang Yi,Zhang Yingwen,Qian Baohua,Li Yanxin,Gu Haihui
Objective The global shortage of platelets presents a significant challenge in healthcare. Although human induced pluripotent stem cells (hiPSCs) offer a renewable source for ex vivo platelet production, the current approach remains constrained by heterogeneity, low yield, and high costs. This study introduces an optimized differentiation scheme (ODS) to improve ex vivo platelet differentiation from hiPSCs. Methods A systematically optimized culture protocol was developed, incorporating: (1) a higher initial dose of embryoid body (EB) cells, (2) refining culture medium, (3) substitution of cytokines with small molecules, and (4) enhancement of megakaryocyte (MK) polyploidization via small-molecule supplementation. Feasibility and effectiveness were evaluated using microscopy, cell counting, flow cytometry, Wright-Giemsa staining, immunofluorescence (IF), and transmission electron microscopy (TEM). Results Increasing the initial EB cell count significantly promoted megakaryocyte production and accelerated the process. A serum-free medium supplemented with human platelet lysate (HPL) was favorable for megakaryocyte generation. Small molecules 740Y-P and butyzamide effectively substituted SCF and TPO for differentiation, while the combination of blebbistatin and 616452 enhanced megakaryocyte maturation. Mature megakaryocytes continuously generated functional platelets that, upon thrombin activation, facilitated fibrin clot formation and contraction in vitro. This method shortened differentiation to 19 days, enhanced output to 1.42 CD41⁺ megakaryocytes and 14.9 platelets per iPSC, and reduced costs by 58.3%. Conclusion We have established a cost-effective strategy for platelet production via hiPSC differentiation, with potential applications in cell therapy and gene editing. Graphical
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产品关联
DOI
PubMed
高影响因子
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2024
·Vol. 121
·期 42
·e2405860121
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IF 9.4
Sze Hang Kwok,Yuejiang Liu,David Bilder,Jung Kim
Tumors can induce systemic disturbances in distant organs, leading to physiological changes that enhance host morbidity. In Drosophila cancer models, tumors have been known for decades to cause hypervolemic “bloating” of the abdominal cavity. Here we use allograft and transgenic tumors to show that hosts display fluid retention associated with autonomously defective secretory capacity of fly renal tubules, which function analogous to those of the human kidney. Excretion from these organs is blocked by abnormal cells that originate from inappropriate activation of normally quiescent renal stem cells (RSCs). Blockage is initiated by IL-6-like oncokines that perturb renal water-transporting cells and trigger a damage response in RSCs that proceeds pathologically. Thus, a chronic inflammatory state produced by the tumor causes paraneoplastic fluid dysregulation by altering cellular homeostasis of host renal units.
研究领域:
医学
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细胞生物学
·
遗传学
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DOI
高影响因子
TEXTILE RESEARCH JOURNAL
·
2024
·
IF 1.6
Chaoxu Han,Yan Guo,Ruirui Yang,Shuyue You,Mina Han
Composite medical dressings are made by combining different materials to achieve functions that cannot be realized by a single material, making it an important research direction for medical dressings. This study utilized the unique grooved structure of lotus stem fibers, and the functionality provided by different preparation processes for each layer of material to prepare a medical dressing that can achieve super-strong liquid absorption and storage effects. The prepared materials were characterized and analyzed through liquid absorption performance tests, Fourier transform infrared spectroscopy and scanning electron microscopy. The results showed that the pore structure of modified chitosan hydrogel, the curled distribution of lotus stem fibers in the material, and the moisture-wicking structure contribute to improving the liquid absorption properties. The composite dressing prepared using the optimal process for each layer of material exhibited moisture absorption and retention rates of 591.34% and 87.99%, respectively, demonstrating excellent liquid absorption properties. The antibacterial properties of the composite dressing were also tested against two common bacteria, and the dressing showed good antibacterial performance. The results indicated that the self-assembled composite medical dressing made from lotus stem fibers meets the preparation requirements, and has good application prospects for use in medical dressings.
研究领域:
材料科学
·
生物医学工程
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DOI
高影响因子
Bone Research
·
2026
·Vol. 14
·期 1
·82
·
IF 20.1
Huang Tiannan,Wu Shali,Qian Wei,Chen Ruiying,Chen Erman,Wang Meizhu,Zhang Cui,Zhou Chenhe,Yu Luyang,Wu Mengjie,Li Yi-Ping,Wu Mengrui
Exhaustion of skeletal stem and progenitor cells (SSPCs) drives age-related delays in fracture repair, yet the upstream regulators of SSPC maintenance are unclear. We identify that core-binding factor β (Cbfβ) in bone marrow Adipoq + cells (BMACs) is essential for maintaining SSPC number and function. Cbfβ deletion in BMACs (CKO) leads to SSPC depletion, including periosteal populations, and impairs bicortical fracture healing in mice. Multi-omics (RNA-seq, CUT&Tag-seq, and ATAC-seq) reveal that Cbfβ preserves chromatin accessibility at DNA repair loci, maintaining genomic stability, preventing BMAC senescence, and mitigating the senescence-associated secretory phenotype (SASP). Senolytic therapy alleviates BMAC senescence, restores SSPC populations, and improves bone repair in CKO mice. In both humans and mice, Cbfβ expression declines with aging, accompanied by increased BMAC senescence. AAV-mediated Cbfβ overexpression restores aging-related bone repair and SSPC decline. These findings reveal a novel mechanism in which Cbfβ in BMACs regulates SSPC maintenance via a senescence/SASP axis, offering a potential therapeutic strategy for age-related bone repair deficits.
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产品关联
DOI
高影响因子
Bioactive Materials
·
2026
·
IF 23.6
Tiantian Xue,Jiabin Zhang,Bin Wang,Fenfang Li,Guipan Chen,Yanteng Xu,Yuechen Luo,Jiali Sun,Yeh-Hsing Lao,Jin Wang,Haixia Wang,Enguo Ju,Xi Xie,Haochen Yao,Mingqiang Li,Yu Tao
Hepatocyte-like cells (HLCs) represent a promising therapeutic approach for acute liver failure (ALF), offering an alternative to primary hepatocytes, which are in short supply. Given the viscoelastic tissue properties of the liver and the growing recognition of the mechanical cues in physiology and disease, we investigated how ALF-induced changes in the liver's mechanical microenvironment might affect HLC-based cell therapy. Here, driven by specific disease-derived mechanical cues, we designed bioinspired hydrogel microspheres with tunable liver viscoelasticity to mimic the mechanical niche of healthy versus ALF liver tissue. We show that a fast-relaxation hydrogel niche mimicking the healthy liver guides the efficient differentiation of human adipose-derived mesenchymal stem cells (hADSCs) into functional HLCs by suppressing the formation of abnormal stress fibers and pathological YAP nuclear translocation through the ROCK axis. In both the carbon tetrachloride-induced and partial hepatectomy-induced ALF mouse models, these mechanically preconditioned HLCs exhibit significant therapeutic efficacy, promoting robust liver regeneration and acting as active mechanical regulators to suppress aberrant host mechanotransduction and remodel the diseased microenvironment. This disease mechanobiology-oriented strategy provides insights into the design of viscoelastic biomaterials for regenerative medicine and demonstrates a promising approach for enhancing cell therapy outcomes in liver failure.
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DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2026
·Vol. 9
·期 15
·7069-7080
·
IF 5.6
Chi Chunyu,Gao Yuantao,Chen Jiazhu,Ge Xuejun,Gao Zexing,Li Yinggang,Yan Shuang
The delayed healing of skin wounds poses significant health risks and increases medical burdens, necessitating the development of multifunctional dressings that can actively monitor and promote the healing process. In this study, a multifunctional CeO 2 /PCL nanofibrous scaffold integrating real-time pH sensing, enhanced stem cell adhesion, and antioxidant functions was developed. A series of composite scaffolds with tunable CeO 2 nanoparticle loadings (7.1–31.6 wt %) were fabricated via a facile one-step electrospinning technique. Notably, the CeO 2 /PCL-13.3 wt % scaffold achieved an optimal balance of tensile strength (1.927 MPa) and elongation at break (70%), fully satisfying clinical wound dressing requirements. Phenol red loading enabled naked-eye pH monitoring (4.0–10.0) via metal−phenolic coordination. Comprehensive evaluations demonstrated that the composite scaffolds exhibited significant antibacterial activity, potent reactive oxygen species scavenging capability, and excellent biocompatibility. The scaffolds maintained over 80% human umbilical cord mesenchymal stem cells viability within 72 h and significantly promoted stem cell migration in wound healing assays. This integrated platform unifies visual diagnostics and therapeutic functions for advanced wound management.
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2026
·Vol. 16
·期 27
·25081-25093
·
IF 6.1
Zhuo Wang,Mengya Yu,Jiong Kong,Mi Zheng,Weifeng Li,Yumei Long,Zuoshan Wang
Solar-driven interfacial evaporation has emerged as a promising technology for freshwater production and energy sustainability. It leverages solar energy to efficiently evaporate water, enabling sustainable seawater desalination and wastewater purification. However, designing efficient evaporators that combine rapid water transport and high salt resistance remains a significant challenge. Drawing inspiration from the long-range ordered vasculature and anti-gravity water management mechanisms of taro stem, we fabricated a biomimetic aerogel featuring vertically aligned microchannels through a unidirectional freezing ice templating method. The aerogel integrates a core–shell SiC@C composite as a high-performance photothermal converter, a mechanically robust PVA/PAM double network forming the channel walls, and hydroxyapatite (HA) nanorods serving as both a thermal insulation skeleton and a mechanical reinforcement. In contrast to conventional aerogels with randomly oriented and tortuous pores, the biomimetic honeycomb architectures with vertically aligned channels endow the material with exceptional water transportation, thereby facilitating efficient salt ion diffusion and mitigating salt crystallization. Under 1 sun illumination (1 kW m−2), the aerogel achieves a high evaporation rate of 3.24 kg m−2 h−1, substantially outperforming most reported evaporators with disordered porous structures. The unique vertical channel configuration ensures continuous and stable desalination performance, highlighting its great potential as an effective solution to address global freshwater scarcity.
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DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2026
·
IF 19.9
Haoyang Ding,Jiaxiang Li,Zhen Chen,Xiangchuan Xu,Lili Hao,Lingyun Sun,Zhongwei Gu,Hongli Mao
As a critical stage in chronic liver disease, liver fibrosis can progress to irreversible damage without timely intervention. Stem cell therapy offers significant therapeutic potential owing to its potent regenerative and immunomodulatory properties. However, its clinical application is challenged by low cell survival, poor targeting, and inadequate paracrine regulation. Here, we present a magnetic hydrogel patch laden with human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) to enhance their antifibrotic efficacy via synergistic magneto-mechanical stimulation. The patch is fabricated through thiol-ene “click” chemistry using multi-thiol-functionalized magnetic nanoparticles as crosslinkers. Molecular dynamics simulations reveal the regulatory mechanism of nanoparticle concentration on the crosslinking topology of the hydrogel network, thereby identifying the optimal formulation with favorable mechanical properties and magnetic responsiveness. Under magneto-mechanical stimulation, the resulting patch significantly enhances the secretion of key antifibrotic factors from hUC-MSCs by activating the PI3K/Akt/mTOR signaling pathway. Moreover, in vitro and in vivo results demonstrate that, under magnetic stimulation, the hUC-MSC-laden magnetic patch significantly inhibits hepatic stellate cell activation, promotes anti-inflammatory macrophage polarization, reduces collagen deposition, and ameliorates the inflammatory microenvironment, thereby effectively reversing liver fibrosis. This study offers a versatile stem cell-based platform for spatiotemporally controlled therapy of liver fibrosis and potentially other fibrotic diseases.
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高影响因子
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