LANGMUIR
·
2022
·
IF 4.331
Hongjiao Wu,Jun Liu,Zhuoyu Chen,Pengcheng Lin,Wentao Ou,Zian Wang,Wei Xiao,Ying Chen,Donglin Cao
Peroxidase-like nanozymes with robust catalytic capacity and detection specificity have been proposed as substitutes to natural peroxidases in biochemical sensing. However, the catalytic activity enhancement, detection mechanism, and application of nanozyme-based biosensors toward l-cysteine (l-Cys) detection still remain significant challenges. In this work, a doped ferrite nanozyme with well-defined structure and surface charges is fabricated by a two-step method of continuous flow coprecipitation and high-temperature annealing. The resulted ferrite nanozyme possesses an average size of 54.5 nm and a zeta-potential of 6.45 mV. A high-performance biosensor is manufactured based on the peroxidase-like catalytic feature of the doped ferrite. The ferrite nanozyme can oxidize the 3,3′,5,5′-tetramethylbenzidine (TMB) with the assistance of H2O2 because of the instinctive capacity to decompose H2O2 into ·OH. The Michaelis–Menten constants (0.0911 mM for TMB, 0.140 mM for H2O2) of the ferrite nanozyme are significantly smaller than those of horseradish peroxidase. A reliable colorimetric method is established to selectively analyze l-Cys via a facile mixing-and-detecting methodology. The detection limit and linear range are 0.119 μM and 0.2–20 μM, respectively. Taking the merits of the ferrite nanozyme-based biosensors, the l-Cys level in the human serum can be qualitatively detected. It can be anticipated that the surface-charged ferrite nanozyme shows great application prospects in the fields of bioanalytical chemistry and point-of-care testing.
研究领域:
生物分析化学
·
即时检测
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纳米技术
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 8
·1477-1485
·
IF 4.3
Yuan Zhenfeng,Li Zhe,Zhao Xiaoxue,Ding Zhenyu,Xia Mingyuan,Dong Xuyang,Ni Pengjuan,Lu Yizhong
Hexavalent chromium is a highly toxic substance, which will pose a serious threat to human life and health and the entire ecosystem. Therefore, it is crucial to establish a simple and rapid detection method for hexavalent chromium. In this work, we fabricated bovine serum albumin–stabilized silver nanocluster (BSA-Ag 13 NC) which exhibited photoresponsive oxidase-like activity, catalyzing the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to the blue oxidized state TMB (oxTMB) in a short time. Interestingly, 8-hydroxyquinoline (8-HQ) can significantly inhibit the color reaction of TMB oxidation while Cr(VI) can interact specifically with 8-HQ to restore this chromogenic reaction. Based on the above facts, a colorimetric sensing system for detecting Cr(VI) was developed. The sensing system shows a wide linear range, and good selectivity, with a low detection limit of 2.32 nM. Moreover, this sensing system could be successfully applied to the detection of Cr(VI) in lake water, tap water, and sewage with satisfactory results.
研究领域:
纳米技术
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环境科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 37
·26102-26110
·
IF 3.9
Jierui Yang,Benqi Chen,Huiting Qiu,Shuang Meng,Yunhui Yang
Ascorbic acid is a nutritional small molecule essential to human life activities and health, playing a vital role in many physiological processes. Fresh fruits and beverages can provide abundant AA to maintain human metabolic balance. Therefore, it is of great significance to develop a nanomaterial with superior nanozyme activity for rapid and convenient detection of ascorbic acid (AA) in fruits and beverages. Herein, a dual-signal sensing platform based on UV-vis absorption and test strip chromaticity for the quantitative determination of AA is presented. The sensing platform is based on the horseradish peroxidase-like activity of Ni3V2O8 nanoflowers, which catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by hydrogen peroxide to the blue oxide TMB (ox TMB). The ox TMB produced by the oxidation has a characteristic absorption peak at 650 nm. In the presence of AA, the blue ox TMB is reduced to colorless TMB, and the quantitative detection of AA can be achieved by detecting the decrease in intensity of the absorption peak by UV-Vis spectrophotometry. Under the optimal experimental conditions, the sensing platform exhibited excellent sensitivity and selectivity. A wide linear range of 0.1 μM to 40 μM with a detection limit of 0.032 μM was obtained. The linear equation is ΔA = 0.02513c + 0.1164 with a correlation coefficient of 0.9979. It showed excellent properties in the detection of real samples of fruit juices and beverages, meanwhile, a method for the rapid detection of AA based on chromaticity change of test strips was constructed with high sensitivity and convenience. The linearity range for the ascorbic acid was 1–50 μM with LOD of 0.42 μM. The developed sensing platform has the capability to quickly and accurately detect ascorbic acid (AA) in fresh fruits and beverages. This proposed method offers a new and promising approach for the rapid and cost-effective detection of ascorbic acid, which has a wide range of potential applications.
研究领域:
纳米技术
·
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Yi Xiao,Pengcheng Huang,Fang-Ying Wu
α-Glucosidase, which directly involves in the metabolism of starch and glycogen and causes an increase in blood sugar level, is the major target enzyme for the precaution and therapy of type II diabetes. Based on the previous work, we adopted a post-synthetic modification method to encapsulate Tb 3+ into Ce-MOF nanozyme which owned mixed valence states. Tb@Ce-MOF displayed induced luminescence characteristic and exceptional oxidase-like activity that could oxidize colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue ox-TMB. α-Glucosidase can hydrolyze the substrate l -ascorbic acid-2- O -α- d -glucopyranosyl (AAG) to generate ascorbic acid (AA), which could increase the Ce 3+ /Ce 4+ redox valence mode in Tb@Ce-MOF, leading to the inhibition of the allochroic reaction of TMB and the decreased absorption of ox-TMB at 652 nm. The energy transfer (EnT) process from Ce 3+ to Tb 3+ will enhance due to the increased Ce 3+ /Ce 4+ mode in Tb@Ce-MOF, which will result in an enhanced fluorescence signal of Tb@Ce-MOF at 550 nm. But the addition of inhibitor acarbose will inhibit the above process. We have constructed a dual-mode detection platform of α-glucosidase and its inhibitor via colorimetric and fluorometric method. The linear range of α-glucosidase were 0.01–0.5 U/mL (colorimetric mode) and 0.8–1.5 U/mL (fluorometric mode), respectively, with a detection limit as low as 0.0018 U/mL. Furthermore, our approach was also successfully employed to the analysis of α-glucosidase in serum samples.
研究领域:
分析化学
·
生物化学
·
材料科学
·
糖尿病研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Ting Ting Li,Hui Xi Li,Qiang Yu,Wen Qing Jia,Shu Ting Li,Wei Zhu,Zhen Chen,Lei Han
It is vital to exploit sensitive and portable means for the reliable assay of acid phosphatase (ACP) activity and its inhibitors screening in clinical diagnosis and drug therapy. Herein, the Pt-Ni alloy nanoparticles (Pt-Ni ANPs) with enhanced oxidase-mimetic activity were successfully prepared and applied to oxidize the achromatous 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxTMB. The oxTMB, as an effective photothermal agent, can transform photon energy to heat under an 808 nm laser illumination. The absorbance and temperature of the reaction solution increased significantly when TMB was oxidized to oxTMB by the oxidase-like Pt-Ni ANPs. However, the ACP can hydrolyze 2-Phospho-L-ascorbic acid trisodium salt (AAP) to produce ascorbic acid (AA), and then the production of AA, as a powerful reductant, can efficiently reduce oxTMB and react with singlet oxygen ( 1 O 2 ) to suppress the oxidase-mimicking activity of Pt-Ni ANPs, causing an obvious decrease in absorption spectrum and temperature of the assay system. A colorimetric and photothermal dual-mode detection platform was established for ACP activity assay by integrating colorimetric and temperature signals. Moreover, the potential application of this strategy was also verified by sensing ACP activity in human serum samples and its inhibitor screening. This dual-mode sensing platform not only improves sensitivity and precision but also meets the needs of diversity detection, which offers new insights for efficient monitoring of ACP activity and inhibitor screening in resource-poor settings.
研究领域:
分析化学
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生物传感器
·
医学诊断
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纳米技术
·
临床化学
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Hanwen Zhang,Jiabin Jin,Xini Wang,Wei Han,Lei Qin,Lin Chen,Xiaoyan Mao,Zhenjiang Liu,Xingwei Xiang
The presence of pesticide residues in aquatic environments poses a significant threat to both aquatic ecosystems and human health. The presence of these residues can result in significant harm to aquatic ecosystems and can negatively impact the health of aquatic organisms. Consequently, this issue requires urgent attention and effective measures to mitigate its impact. However, developing sensitive and rapid detection methods remains a challenge. In this study, an all-in-one test strip, which integrated bioenzymes, nanoenzymes, and a chromogen, was developed in combination with an enzyme labeling instrument for a highly sensitive and convenient sensing of malathion residues. The oxidase activity of heme chloride (Hemin) in the strip can catalyze the oxidation of H 2 O 2 and 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue-colored oxide. Simultaneously, the alkaline phosphatase (ALP) present in the strip can break down l -ascorbic acid-2-phosphate to produce ascorbic acid (AA). This AA then acts to reduce the oxidized form of TMB, turning it into a colorless substance and leading to the disappearance of its fluorescent signal. In the presence of a pesticide, the activity of ALP is inhibited and formation of AA is blocked, thereby preventing the reduction of oxidized TMB and producing a colored signal. According to this principle, the integrated test strip detected the target pesticide with high performance as per the optical density value determined via an enzyme marker. The detection limit of the test strip was 0.209 ng/mL with good sensitivity. The method was used for detecting malathion in actual river water samples, and the recoveries were in the range of 93.53 %–96.87 %. The newly devised technique effectively identified malathion in samples of natural water. This research has introduced a novel approach for the precise and convenient surveillance of pesticide remnants. Additionally, these discoveries could inspire the advancement of proficient multi-enzyme detection systems.
研究领域:
环境科学
·
分析化学
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生物技术
·
水质监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 21
·4031-4039
·
IF 7.571
Xu Li,Kang Cui,Mingzhen Xiu,Chenxi Zhou,Li Li,Jing Zhang,Shiji Hao,Lina Zhang,Shenguang Ge,Yizhong Huang,Jinghua Yu
In this work, novel dual-mode lab-on-paper devices based on in situ grown WO3/BiVO4 heterojunctions onto cellulose fibers, as signal amplification probes, were successfully fabricated by the integration of photoelectrochemical (PEC)/colorimetric analysis technologies into a paper sensing platform for the ultrasensitive detection of alpha-fetoprotein (AFP). Specifically, to achieve an impressive PEC performance of the lab-on-paper device, the WO3/BiVO4 heterojunction was in situ grown onto the surface of cellulose fibers assisted with Au nanoparticle (Au NP) functionalization for enhancing the conductivity of the working zone of the device. With the target concentration increased, more immune conjugates could be captured by the proposed paper photoelectrode, which could lead to a quantitative decrease in the photocurrent intensity, eventually realizing the accurate PEC signal readout. To meet the requirement of end-user application, a colorimetric signal readout system was designed for the lab-on-paper device based on the color reaction of 3,3′5,5′-tetramethylbenzidine (TMB) oxidized by WO3/BiVO4 nanoflowers in the presence of H2O2. Noticeably, it is the first time that the WO3/BiVO4 heterojunction is in situ grown onto cellulose fibers, which enhances the sensitivity in view of both their PEC activity and catalytic ability. By controlling the conversion process of hydrophobicity and hydrophilicity on the lab-on-paper device combined with diverse origami methods, the dual-mode PEC/colorimetric signal output for the ultrasensitive AFP detection was realized. Under optimal conditions, the proposed dual-mode lab-on-paper device could enable the sensitive PEC/colorimetric diagnosis of AFP in the linear range of 0.09–100 ng mL−1 and 5–100 ng mL−1 with the limit of detection of 0.03 and 1.47 ng mL−1, respectively.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
材料科学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 30
·6459-6468
·
IF 5.344
Xiaodan Zhang,Yuwan Lu,Qiumeng Chen,Yuming Huang
A self-templated strategy was adopted to design hollow Co3O4/MO3 (M = Mo, W) mixed-metal oxides via the Mo or W doping of ZIF-67, and subsequent pyrolysis under an atmosphere of air at a low temperature of 450 °C. The hollow Co3O4/MO3 (M = Mo, W) mixed-metal oxides displayed tunable oxidase-like and peroxidase-like activities able to efficiently catalyze the oxidation of TMB to generate a deep blue color in the absence or presence of H2O2. Relative to that of the un-doped Co3O4, the oxidase mimic activity of the Mo-doped Co3O4 increased to 1.3 to 2.1-fold, while its peroxidase mimic activity increased to 7.1 to 19.9-fold, depending on different Mo doping amounts. The oxidase mimic activity of the W-doped Co3O4 increased to 2.1 to 2.3-fold, while its peroxidase mimic activity increased to 4.8 to 5.9-fold, depending on the different W doping amounts. The Mo- and W-doped Co3O4 nanohybrid exhibited both higher O2 and H2O2 activating capability, and their H2O2 activating capacity was superior to the O2 activating capability. Furthermore, the Mo- and W-doped Co3O4 nanohybrids exhibited similar O2 activating abilities, while the Mo-doped one displayed a higher H2O2 activating capability than the W-doped one. The discrepant peroxidase-like nature of Mo- and W-doped Co3O4 nanohybrids is likely attributed to their different catalytic mechanisms. The peroxidase-like activity of Mo-doped Co3O4 is highly related to the ˙OH free radical, while that of W-doped Co3O4 is likely ascribed to the electron transfer between TMB and H2O2. The Km values of Co3O4/MoO3 for TMB and H2O2 were 0.0352 mM and 0.134 mM, which were 3.2- and 1.9-fold lower than that of pure Co3O4, respectively. A Co3O4/MoO3-based colorimetric platform was developed for the determination of H2O2 in the 0.1–200 μM range, with a limit of detection of 0.08 μM (3σ). Based on the thiocholine (TCh) inhibition of the excellent peroxidase-like activity of Co3O4/MoO3 and the TCh generation via acetylcholinesterase (AChE) catalyzed hydrolysis of acetylthiocholine chloride (ATCh), the colorimetric platform was extended to screen AChE activity and its inhibitor.
研究领域:
纳米材料
·
酶模拟
·
分析化学
·
生物传感器
·
催化
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 5
·1-9
·
IF 5.705
Yu Xinhui,Lin Yaohui,Wang Xusheng,Xu Liangjun,Wang Zongwen,Fu FengFu
An exonuclease-assisted multicolor aptasensor was developed for the visual detection of ochratoxin A (OTA). It is based on the etching of gold nanorods (AuNRs) mediated by a G-quadruplex-hemin DNAzyme. A DNA sequence (AG4-OTA) was designed that comprises a hemin aptamer and an OTA aptamer. OTA binds to AG4-OTA to form an antiparallel G-quadruplex, which halts its digestion by exonuclease I (Exo I) from the 3′-end of AG4-OTA. Thus, the retained hemin aptamer can bind to hemin to form a G-quadruplex-hemin DNAzyme. This DNAzyme has peroxidase-like activity that catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 to produce its diimine derivative (TMB 2+ ) in acidic solution. TMB 2+ can etch the AuNRs by oxidizing Au(0) into Au(I). This results in the generation of rainbow-like colors and provides a multicolor platform for the visual detection of OTA. The assay is based on the use of a single isolated aptamer and possesses obvious advantages such as multi-color visual inspection, relatively high sensitivity and accuracy. It can be used to detect as little as 30 nM concentrations of OTA by visual observation and even 10 nM concentrations by spectrophotometry. The method was successfully applied to the determination of OTA in spiked beer where it gave recoveries of 101–108%, with a relative standard deviation (RSD, n = 5) of <5%.
Schematic of an exonuclease-assisted multicolor bioassay based on the G-quadruplex-hemin DNAzyme-mediated etching of gold nanorods (AuNRs). It enables visual detection of ochratoxin A (OTA) with a detection limit of 30 nM.
研究领域:
分析化学
·
食品科学
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纳米技术
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Linlin Zhang,Jie Wang,Chenglong Zhao,Feijin Zhou,Cheng Yao,Chan Song
The nanozymes-based sensing platforms have been attracted in recent years due to their outstanding performances in the practical applications. In this work, the magnetic Fe nanoparticles (NPs) with peroxidase-like activity was developed for the fast detection of glutathione (GSH). The Fe NPs were facilely prepared through the reduction of iron trichlorrde hexahydrate (FeCl 3 ‧6H 2 O) by sodium borohydride (NaBH 4 ) at room temperature , exhibiting a merit of simplicity. Significantly, the achieved Fe NPs have high peroxidase-like catalytic activity . At the room temperature, the colorimetric substrates such as 3,3′,5,5′-tetramethylbenzidine (TMB) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) can be rapidly oxidized by Fe NPs with the help of H 2 O 2 . After incubated for just 30 s, the catalytic system could achieve a colored solution. Thus, the Fe NPs-based sensing systems can be employed in the development of the colorimetric biosensors with the naked-eye detection.
研究领域:
纳米技术
·
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Minyang Zhao,Xiaodong Lin,Xiao Zhou,Yujie Zhang,Haotian Wu,Yaqing Liu
Simple and efficient identification of multiple bacteria and sterilization in real time is of considerable significance for clinical diagnostics and quality control in food. Herein, a novel chemical-tongue sensor array with 3,3′,5,5′-tetramethylbenzidine (TMB) as a single probe was developed for bacterial identification and photothermal elimination. The synthesized bimetallic palladium/platinum nanoparticles (Pd/PtNPs) present excellent catalytic capability that can catalyze TMB into oxidized TMB (oxTMB) with four feature absorption peaks. Bacteria have different ability on inhibiting the reaction between TMB and Pd/PtNPs. With the absorbance intensity of oxTMB at the four feature peaks as readout, nine kinds of bacteria including two drug-resistant bacteria can be successfully distinguished via linear discriminant analysis. Remarkably, oxTMB exhibits excellent photothermal properties and can effectively kill bacteria in real time under near-infrared laser irradiation. The strategy of selecting TMB as a single probe simplifies the experimental operation and reduces the time cost. Furthermore, the developed sensing system was used to promote the wound healing process of MRSA-infected mice in vivo. The investigation provides a promising simple and efficient strategy for bacterial identification and sterilization with a universal platform, which has great potential application in clinical diagnosis and therapy.
研究领域:
临床诊断
·
食品质量控制
·
纳米技术
·
生物医学工程
·
光热治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2022
·Vol. 147
·期 18
·4105-4115
·
IF 5.227
Xin Tan,Wancui Xie,Qi Jia,Fangyuan Zhao,Wei Wu,Qingli Yang,Xiudan Hou
In this work, a simple, sensitive and selective colorimetric method was established for the detection of profenofos. Firstly, novel flower-shaped AuPtRh trimetallic nanospheres were synthesized via a simple one-pot method, and had outstanding peroxidase catalytic activity. AuPtRh nanospheres with a great specific surface area were linked with an aptamer via Au–S and Pt–S bonds to specifically recognize profenofos. A graphene oxide grafted stainless-steel mesh (SSM–GO) was prepared to be a carrier and the aptamer–AuPtRh was nonspecifically adsorbed on the surface of SSM–GO, which was to be the capture probe for the detection of profenofos in real samples. They were characterized and confirmed by transmission electron microscopy, atomic force microscopy, etc. Through the investigation of the catalytic performance on the basis of the Michaelis equation, the Vmax of AuPtRh nanospheres was 22.27 × 10−8 M s−1, and Km was 0.6632 mM, which indicated that the affinity of AuPtRh nanospheres was relatively higher than that of horseradish peroxidase and Au NPs. In the presence of profenofos, the aptamer–AuPtRh would specifically combine with profenofos, which would further detach from SSM–GO. Then, it was introduced into the 3,3′,5,5′-tetramethylbenzidine/H2O2 (TMB/H2O2) system to form blue oxTMB. The linear range of this colorimetric biosensor was 1–300 ng L−1 and the limit of detection was 0.725 ng L−1. It also had good recovery and anti-interference ability in real samples, which provided a new strategy for the rapid detection of pesticides.
研究领域:
分析化学
·
纳米技术
·
环境科学
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）