ACS Biomaterials Science & Engineering
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2017
·
IF 3.234
Hong-Yin Wang,Jie Sun,Liu-Yuan Xia,Yan-Hong Li,Zhan Chen,Fu-Gen Wu
Immunofluorescence staining is a crucial tool for studying the structure and behavior of intracellular proteins and organelles. During the staining process, the permeabilization treatment is usually required to enhance the penetration of a fluorescent antibody into the cells. However, since most of the membrane imaging dyes as well as the membrane lipids will detach from the cell surface after permeabilization, membrane labeling using these dyes is not compatible with immunofluorescence staining. Herein, by linking cholesterol-polyethylene glycol (PEG-Chol) and fluorescein isothiocyanate (FITC) with the amine-rich glycol chitosan (GC), we prepared a multifunctional polymeric construct, GC-PEG Chol-FITC, and realized permeabilization-tolerant plasma membrane imaging. Owing to the presence of abundant amine groups in the labeling reagent and the membrane proteins/lipids, the addition of paraformaldehyde in the fixation step induces the amine-cross-linking between the labeling reagents and the membrane proteins/lipids, thus preventing the detachment of fluorophores from the cell surface after permeabilization. Besides, the large molecular weight effect of the imaging reagent may also account for its antipermeabilization property. Furthermore, by combining immunofluorescence staining with the plasma membrane labeling by GC-PEG Chol-FITC, we simultaneously imaged the plasma membrane and cytoskeletons, and clearly observed metaphase cells and binucleated cells. The concept of using amine-rich polymeric dyes for plasma membrane imaging will inspire the development of more permeabilization-resistant membrane labeling dyes with better performance, which can realize simultaneous membrane and intracellular protein imaging and facilitate the future studies of membrane–intracellular protein interactions.
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2017
·Vol. 106
·期 1
·192-200
·
IF 3.076
Jing-Xiao Chen,Jing Yuan,Ya-Ling Wu,Ping Wang,Peng Zhao,Guo-Zhong Lv,Jing-Hua Chen
In this study, a series of poly(ethylene glycol)/collagen (PEG/Col) double network (DN) hydrogel is fabricated from PEG and Col. Results of the compressive strength test indicate that the strength and toughness of these DN hydrogels are significantly enhanced. The fracture strength of PEG/Col DN hydrogels increases by 9- to 12-fold compared with that of PEG single network (SN) hydrogel, and by 36- to 48-fold compared with that of Col SN hydrogel. Taking advantage of both PEG and Col building blocks, the PEG/Col DN hydrogels possess a strengthened skeleton. Moreover, the water-storage capability and favorable biocompatibility of Col are effectively maintained. Given that the DN hydrogels can provide the appropriate environment for the adhesion, growth, and proliferation of MC3T3-E1 cells, PEG/Col DN hydrogels have potential as a load-bearing tissue repair material. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 192–200, 2018.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2012
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IF 2.237
Jun Li,Chenjia You,Lifang Chen,Yinmei Ye,Zhiwen Qi,Kai Sundmacher
To reduce the high energy consumption and equipment corrosion in conventional processes of CO2 capture with aqueous amine solutions, the mixed nonaqueous solvents of monoethanolamine (MEA), diethanolamine (DEA), and diglycolamine (DGA) with polyethylene glycol (PEG) as cosolvent were explored for CO2 capture. The dynamic experiments of CO2 absorption and desorption were carried out to evaluate the performance of the studied nonaqueous solutions. It demonstrated that the mixed solutions of amines and PEG exhibited higher CO2 cyclic capacity and regeneration efficiency compared with the only aqueous amine solutions. Especially, the solution of 3 mol/L DGA-PEG200 exhibits a high cyclic capacity of 0.438 mol CO2/mol DGA and a high regeneration efficiency of 94.6%, which indicates its great potential in industrial application. Moreover, the very low vapor pressure of PEG helps the mixed solution for CO2 capture with reduced corrosion, energy consumption, and environmental pollution.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2012
·Vol. 128
·期 5
·2817-2822
·
IF 1.289
Guixiang Zhu,Ying Wang,Weipu Zhu,Kui Zhu,Xiaoyan Xu,Zhiquan Shen
In this article, we report the facile one-pot synthesis of poly(decamethylene succinate) (PDS) with multiple hydroxyl groups by direct polycondensation with malic acid (MA) as the functional monomer and dysprosium triflate as the chemoselective catalyst at 80°C. The secondary hydroxyl groups of malic units were inactive during the polycondensation process. The density of hydroxyl groups of the copolymer could be well-controlled by the molar ratio of succinic acid and MA in the feed. These hydroxylated PDSs were grafted by poly(ethylene glycol) (PEG) through a simple esterification reaction; this resulted in amphiphilic PDS- g -PEG copolymers, which could undergo micellization in aqueous media to form nanosized aggregates with diverse morphologies and diameters. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013.
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ChemistrySelect
·
2024
·Vol. 9
·期 32
·e202401140
·
IF 1.9
Yuanxin Man,Hui Nan,Jianzhe Ma,Zhike Li,Xianlan Wang,Pan Yang,Caihong Xue,Jingyuan Zhou,Heqi Li
Graphical Polyethylene glycol 10000 (PEG-10000) was selected as a dispersant for γ-AlOOH nanowhiskers in the PE coating slurry. The AlOOH-PEG/PE separator maintains its structural integrity even after a 0.5 hour test at 200 degrees Celsius, and lithium-ion battery half-cells assembled with it exhibited a capacity of 73.9 % at a 5 C rate. In this study, a γ-boehmite (γ-AlOOH) – PEG/polyethylene (PE) lithium-ion battery separator was fabricated using a dip-coating method, with γ-AlOOH nanowhiskers being synthesized directly through a hydrothermal process,and polyethylene glycol 10000 (PEG-10000) was chosen for the first time as a dispersant for the γ-AlOOH nanowhiskers in the PE coating slurry{Lee, 2014 #7}. Simultaneously, the membrane's performance was characterized, confirming the significant dispersion effect of PEG-10000, resulting in a porosity as high as 64 % in the AlOOH-PEG/PE, a substantial increase compared to the PE separator (40 %). The AlOOH-PEG/PE separator maintains its structural integrity even after a 0.5 h test at 200 °C, showcasing outstanding stability and significantly improved safety. Besides, the LIB half cells assembled with the PE/AlOOH-PEG separator exhibited remarkable performance, with a capacity of 73.9 % in power capability (at 5 C rate) and a 97.1 % discharge capacity retention rates in cycle performance (after 400 cycles, at 2 C rate). Furthermore, lithium iron phosphate (LiFePO 4 ) batteries utilizing the AlOOH-PEG/PE separator demonstrated significantly enhanced cycling stability and rate capacity.
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PROCESS BIOCHEMISTRY
·
2015
·
IF 2.516
Jiefeng Xiao,Zhibao Huo,Dezhang Ren,Song Zhang,Jiang Luo,Guodong Yao,Fangming Jin
A novel and efficient process for the production of 1,2-propylene glycol with high productivity and high selectivity from biomass-derived lactic acid over CuO was investigated. As a result, CuO exhibited a high catalytic activity while Zn acted as a reductant for the conversion of lactic acid to 1,2-propylene glycol. Among the various reaction parameters, such as catalysts, reductants, temperature, water filling and reaction time we investigated, a 93% yield of 1,2-propylene glycol in the presence of 0.83 mmol lactic acid, 5 mmol CuO and 30 mmol Zn with 35% water filling at 250 °C for 2 h was achieved. In addition, a carbon balance at optimum reaction conditions by TOC indicated that approximately 99% lactic acid was converted into liquid products in the transformation. In situ formed hydrogen by the oxidation of Zn in water is effective for the transformation compared to added gases hydrogen. The proposed mechanism for the conversion of lactic acid to 1,2-propylene glycol over CuO is also discussed.
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BIOMACROMOLECULES
·
2017
·
IF 5.246
Lin Xiao,Lixia Huang,Firmin Moingeon,Mario Gauthier,Guang Yang
A biodegradable micellar drug delivery system with a pH-responsive sheddable PEG shell was developed using an acetal-linked poly(ethylene glycol)-block-polylactide (PEG-a-PLA) copolymer and applied to the tumoral release of paclitaxel (PTX). The micelles with a diameter of ∼100 nm were stable in PBS at pH 7.4, started shedding the shell and aggregating slowly at pH 6.5, and decomposed faster at pH 5.5. PTX-loaded micelles (M-PTX) with a drug loading of 6.9 wt % exhibited pH-triggered PTX release in simulated tumoral acidic environments corresponding to the extracellular and intracellular spaces. In vitro experiments showed that the micelles were noncytotoxic to different cell lines, while M-PTX inhibited the proliferation and promoted the apoptosis of Hela cells. An in vivo study with Hela tumor-bearing mice indicated that M-PTX efficiently inhibited tumor growth. Because of these properties, the PEG-a-PLA micellar system appears to have bright prospects as a tumor-targeting drug carrier.
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NEW JOURNAL OF CHEMISTRY
·
2015
·Vol. 39
·期 9
·7033-7039
·
IF 3.086
Siqiao Li,Renfeng Pan,Yamina Ait Mehdi,Deli Xiao,Hua He
In this study, we propose the first use of polyethylene glycol (PEG) as a carbon precursor via a spontaneous exothermic reaction involving phosphorus pentoxide (P2O5) to synthesize fluorescent carbon nanoparticles (FCNPs). The unique FCNPs, which emit bluish violet light under UV light, have a diameter of less than 10 nm and a high quantum yield of 18.4%. Moreover, the FCNPs without further treatments show excellent thermosensitivity, excitation-dependent multicolor emission, high solubility in water, and superior photostability. This facile, economical and convenient route is expected to inspire more research into the origin and applications of FCNPs, as well as delivering FCNP-based materials.
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CHEMICAL PAPERS
·
2012
·Vol. 67
·期 4
·423-428
·
IF 1.096
Zhu Guo-Quan,Wang Fa-Gang,Liu Yu-Ying,Gao Qiao-Chun
A series of water-soluble carboxymethyl chitosan (CMCS)/poly(propylene glycol) (PPG) blend films with various CMCS/PPG mole ratios were prepared by the solution casting method. Morphology of the CMCS/PPG blend films was investigated by scanning electron microscopy (SEM). Thermal, mechanical, and chemical properties of the CMCS/PPG blend films were studied by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), tensile tests, and contact angle tests. It was found that the introduction of PPG can markedly affect the morphology and the properties of CMCS films.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
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2012
·
IF 2.814
Yufei Ai,Yaohua Wei,Jun Nie,Dongzhi Yang
The catecholic amino acid 3,4-Dihydroxyphenyl- l -alanine (DOPA) is believed to be responsible for the water-resistant adhesive characteristics of mussel adhesive proteins (MAPs). In this paper, the synthesis of three-armed poly(ethylene glycol)s with dopamine endgroups (PEG–DOPAs) using acylation reaction and Michael addition reaction was described, and PEG–DOPAs was used as bioadhesive under the UV irradiation. The structures of PEG–DOPAs were characterized by FTIR, 1 H NMR spectroscopy. The adhesion properties of PEG–DOPAs were investigated and results were significantly superior to 0.05 MPa of the commercially available fibrin adhesives. Moreover, the adhesive strength of PEG–DOPAs (Mw 3000) increased from 0.587 MPa to 1.82 MPa, PEG–DOPAs (Mw 20,000) increased from 0.338 MPa to 1.92 MPa with the elongation of the binding time, severally. The photopolymerization kinetic study was taken to evaluate gelation time of bioadhesive indirectly. The cytotoxicity of photocured PEG–DOPAs hydrogels for L929 cells was evaluated by the fluorescence microscopy and MTT (3-[4-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; Thiazolyl blue) assay, respectively. The results showed that PEG–DOPAs were biocompatible and less cytotoxicity toward the growth of L929 cells. The swelling properties of PEG–DOPAs hydrogels were also tested. PEG–DOPAs (3000) reached swelling equilibrium within 2 h at 37 °C, and PEG–DOPAs (20,000) within 6 h at 37 °C.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2014
·Vol. 33
·期 2
·245-255
·
IF 1.42
Zhang Di,Yin Jun,He Fei,Ge Ni-lin,Wu Zong-quan,Ding Yun-sheng
We report on the preparation of a well-defined star-shaped tricationic ionic liquid possessing three arms of poly(ethylene glycol) functionalized imidazolium rings. Remarkable solubility was found in most of the organic solvents we used. Thermogravimetric analysis (TGA) exhibited excellent thermal stability and two distinct decomposition temperatures were attributed to two kinds of chemical degradation. Differential scanning calorimetry (DSC) was further employed to investigate the thermal phase transitions, that three different signals ( T
g , T
c , and T
m ) were shown upon the second heating process. Moreover, CH 2 Cl 2 solution of the ionic liquid expressed an excitation-wavelength dependent fluorescence response, leading to the facile modulation of photoluminescence behavior. This work represents an example of utilizing molecular design to construct novel ionic liquids and endow further potential to be used in the engineering materials.
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JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
·
2013
·
IF 3.187
Yueling Cao,Junwei Wang,Maoqing Kang,Yulei Zhu
Ni–WO 3 /SBA-15 catalysts were prepared by an impregnation way and applied to the hydrogenolysis of cellulose in aqueous solution. The effect of nickel and WO 3 loading, catalysts reduction temperature and time on cellulose conversion were investigated. Up to 70.7% ethylene glycol yield was obtained over 3%Ni–15%WO 3 /SBA-15 catalysts. Several physicochemical methods such as XRD, Raman spectroscopy, NH 3 -TPD, H 2 -TPR and XPS were used to determine the character of catalysts. Based on these studies, it was found that there was a strong electronic interaction between NiO and WO 3 , which not only promoted the reduction of WO 3 , but restrained the reduction of NiO. Together with the experimental results, it can be concluded that the WO 3− X , which may be the active species for the selective cleavage of C C bond, was formed after catalysts pretreatment process. Additionally, a possible catalytic mechanism was proposed.
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