JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2013
·
IF 2.906
Dong-Fang Li,Hao-Chen Ding,Tao Zhou
A commercially available trypsin–chymotrypsin mixture was covalently immobilized onto modified polyvinyl chloride (PVC) microspheres, which were activated by the subsequent treatment of PVC microspheres with ethylenediamine and glutaraldehyde. The immobilized mixed protease was characterized by FT-IR and SEM analyses. Immobilization conditions were optimized by Box–Behnken design and the response surface method. The activity of the immobilized mixed protease prepared under optimal conditions (pH 6.6, 23 °C, 2 h) reached 1341 U/g. Compared with the free form, the immobilized enzyme possesses a slightly higher optimal pH value and a wider pH-activity profile, superior thermal stability, and a higher Km value. Reusability of the immobilized mixed protease indicated that >70% of the original activity was retained after having been recycled six times.
研究领域:
生物化学
·
酶工程
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PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2026
·
IF 2.8
Kun Chen,Zhuang Zhou,Dazhi Wang,Shaoyin Zhang
Free glucose oxidase (GOx) exhibits high specificity and catalytic efficiency under mild conditions; however, its practical application is limited by poor stability and reusability. In this study, a magnetic nanofiber carrier was fabricated from carboxyl-functionalized polyaryl ether sulfone (PAES-COOH) nanofibers via a facile acid precipitation method. The preparation parameters of the magnetic PAES-COOH nanofiber carrier (MNPAES) and the immobilization conditions for GOx were systematically optimized. The structural and surface properties of PAES-COOH nanofibers and MNPAES were characterized by SEM, FT-IR, XPS, 1 H NMR, GPC, and BET analyses. Compared with pristine nanofibers, MNPAES exhibited an increased specific surface area (from 9.5 to 21.1 m 2 /g) and an enlarged average pore size (from 2.3 to 123 nm), facilitating enzyme immobilization. GOx immobilized on MNPAES (GOx@MNPAES) showed a lower apparent K m value and a higher V max / K m ratio than free GOx, indicating enhanced apparent catalytic efficiency. In addition, GOx@MNPAES demonstrated good storage stability, rapid magnetic separation, and high reusability, retaining approximately 60% of its initial activity after 20 cycles. These results suggest that magnetic PAES-COOH nanofibers are effective carriers for GOx immobilization.
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高影响因子
ANNALS OF NUCLEAR ENERGY
·
2022
·
IF 1.81
Dong Wu,Guilin Wei,Xiaoyan Shu,Yi Liu,Wenhong Han,Zhentao Zhang,Shunzhang Chen,Yi Xie,Fen Luo,Xirui Lu
The treatment of radioiodine produced from nuclear industry is a key priority. It is significant to understand the phase evolution and microstructure transformation of the matrix in the field of radioiodine immobilization. In this work, the iodine wastes (silver-coated silica gel doped with iodine waste) were immobilized by B 2 O 3 -Bi 2 O 3 -ZnO at different low sintering temperatures (500, 550, and 600 ℃, respectively). The effect of iodine content in silver-coated silica gel on the phase evolution for the sintered matrix was investigated. Based on the FT-IR results, the changing of microstructure transformation affects by temperature and composition were also studied. These results demonstrate that the simulated iodine waste was gradually immobilized in the glassy phase as the sintering temperature increased. Moreover, under the effect of sintering temperature and material composition, part of B-O-B in [BO 3 ] unit transformed into B-O-B in [BO 4 ], resulting in more iodine wastes were immobilized in the glassy phase. This work mainly reveals the phase evolution and microstructure transformation of the iodine waste matrix sintered at low sintering temperatures.
研究领域:
核废料处理
·
材料科学
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陶瓷与玻璃
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放射化学
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高影响因子
WATER ENVIRONMENT RESEARCH
·
2019
·Vol. 91
·期 7
·565-572
·
IF 1.24
Jingge Shang,Jie Gao,Junting Xi,Yingjie Wang,Fangyu Ji,Ruijun Li
A graphene oxide (GO)—nanoscale zerovalent iron (nZVI)—biochar composite (GO-nZVI/BC) was synthesized prior to characterization by X-ray diffraction (XRD), vibrating sample magnetometer (VSM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy analyses. Batch experiments were performed at different initial Cr(VI) concentrations, contact times, and solution pH values. The effects of coexisting anions and chelating agents were also examined. The results indicated that the removal of Cr(VI) was highly pH-dependent and reached a maximum capacity at pH of 2. The equilibrium data were fitted well with the Langmuir isotherm model, and the kinetic data fitted better with the pseudo-second-order kinetic model. The increasing concentrations of EDTA in aqueous solutions were favorable to the removal of Cr(VI), while significantly inhibited adsorption. Furthermore, the GO-nZVI/BC maintained ~84.5% of its original capacity after aging in the air for 25 weeks. Based on the removal efficiency, GO-nZVI/BC can be considered to be an effective material for water treatment applications. Practitioner points Biochar-supported graphene oxide-coated nanoscale zerovalent iron (GO-nZVI/BC) was synthesized and used to treat Cr(VI) from solution. Cr(VI) removal was pH-dependent and obeyed the Langmuir isotherm model and pseudo-second-order model. GO-nZVI/BC maintained ~84.5% of its original capacity after aging for 25 w in the air. Graphical TEM and Magnetic Field of GO-nZVI/BC and its removal capacity of Cr(VI).
研究领域:
环境科学
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材料科学
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水处理技术
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污染物修复
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高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2022
·
IF 8.91
Changsheng Jin,Zhongwu Li,Mei Huang,Xiang Ding,Mi Zhou,Changqing Cai,Jia Chen
Cd pollution in sediments poses severe threats to environmental safety and human health. Mn oxides have potential merit for the remediation of Cd pollution in sediment but have not received enough attention. Although Mn oxides have proven effective as adsorbents for removing heavy metals from water/wastewater, the performance and the underlying mechanism of Cd immobilization in sediments by Mn oxides remain unclear. Here, three crystallographic Mn oxides δ-MnO 2 , γ-MnOOH, and Mn 3 O 4 were used as amendments to investigate their potential for the in situ immobilization of Cd in lake sediment . Experimental data showed that when the sediment samples were treated with synthesized Mn oxides at dosages of 2% and 6% (w/w) for 56 days, the TCLP (toxicity characteristic leaching procedure) leachable Cd in the sediment decreased by 43.9–66.81%, and the PBET (physiologically based extraction test) extractable Cd decreased by 45.16–99.40%. Additionally, the acid-soluble fraction of Cd was partially transformed to a residual fraction, resulting in a 27.55–35.49% decrease in acid-soluble Cd and a 25.16–30.36% increase in the residual Cd fraction. Sediment pH and oxidation-reduction potential were important factors affecting the bioavailability of Cd in the remediation process. Furthermore, scanning electron microscopy , X-ray diffractometer , Fourier transform infrared spectroscopy , and X-ray photoelectron spectroscopy analysis illustrated that the interaction between the amendment and Cd mainly involved complexation with O-containing groups, ion-exchange as > OCd + , and precipitation with carbonate. The efficient remediation capacity and associated mechanism for Mn oxides provide insights for the improved restoration of heavy metal-contaminated sediment.
研究领域:
环境科学
·
地球化学
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Yue Xu,Zhao Wang,Yang Liu,Zizhen Wu,Shaoxiong Li
The application of modified phosphogypsum (MPG) as a soil amendment has emerged as a promising strategy for its large-scale utilization, yet the potential environmental risk of fluoride (F − ) release during this process has been largely overlooked. This study systematically demonstrated that MPG-amended soils pose a persistent F⁻ leaching hazard, with leachate contents reaching up to 3.0 mg L −1 , which was three times the Class III limit of Chinese Groundwater Quality Standards. To address this risk, Nocardia sp. X10 was isolated and its efficient F − immobilization mechanisms were elucidated. Direct quantification of extracellular polymeric substances (EPS) embedded within the biominerals (30.7–67.7 mg L −1 ) provided evidence that EPS served as nucleation templates for fluorine-containing minerals. Comprehensive characterization identified the biominerals as a co-precipitated assemblage of Ca 5 (PO 4 ) 3 F, CaF 2 , and CaCO 3 , intimately associated with EPS. Quantitative analysis of contributions revealed that microbially mediated mineralization dominated F − immobilization. Calcium (Ca 2+ ) and phosphate (PO 4 3− ) acted as essential mineral precursors, with F − immobilization efficiency increasing from 8.6% (F − alone) to 67.9% and 77.1% (Supplement Ca 2+ and PO 4 3− successively). Soil column validation confirmed that strain X10 achieved superior F − immobilization (65.7%) compared to chemical passivators (57.2% and 61.8%), consistently maintaining leachate F − below regulatory limits. The findings will help reveal the potential environmental risks and the biological immobilization mechanisms involved in the resource utilization of industrial by-products.
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2026
·
IF 6.4
Zhengyun Xie,Like Ning,Farzad Seidi,Shicun Jin,Zhulan Liu,Chao Liu,Huining Xiao
Functionalized nanosilica is used to carry agrochemical IAA via disulfide bonds. • Under GSH environment, SiO 2 -S-S-IAA exhibits significant redox responsiveness. • The -SH on the surface of SiO 2 can form complexes with various heavy metal ions. • Dual-functional nanocarrier allows sustained IAA release and capture of metal ions. Owing to the low efficiency and lack of target specificity in the application of agrochemicals, e.g., plant growth regulators, the resulting environmental pollution and resource waste have raised enormous concerns over environments and human health. Herein, a redox-responsive nanosilica carrier (SiO 2 -S-S-IAA) was developed for efficient release of 3-indoleacetic acid (IAA), by functionalizing the surface of nanosilica with 3-mercaptopropyltrimethoxysilane (MPTMS), followed by the covalent coupling of IAA via redox-cleavable disulfide bonds. Under the high level of glutathione (GSH) in the plant microenvironment, IAA was released in a controlled way through disulfide bond cleavage, thus achieving redox response. In vitro controlled-release experiments confirmed that the release amount of IAA was significantly higher in the environment of 10 mM GSH, more than 10 folds at pH 5.5. Meanwhile, the thiol groups generated after release could form complexes with various heavy metal ions (Hg 2+ , As 3+ /As 5+ , Cd 2+ , Pb 2+ ), with the highest adsorption efficiency towards Hg 2 + up to 57.95%. More importantly, in planta experiments under cadmium stress have demonstrated that SiO 2 -S-S-IAA not only promoted rice seedling growth but also significantly reduced Cd 2+ accumulation in shoots, effectively inhibiting its transfer to edible parts. This work establishes a dual-functional nanocarrier platform that combines targeted agrochemical delivery with in situ heavy metal immobilization, offering a promising strategy for sustainable and precise crop protection. Download: Download high-res image (185KB) Download: Download full-size image
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高影响因子
BIORESOURCE TECHNOLOGY
·
2026
·
IF 8.2
Yuchong Liu,Guan Wang,Changwen Zhao,Wantai Yang
Visible-light graft polymerization enables topology-controlled polymer brushes. • Linear-bottlebrush brush achieves highest enzyme loading (0.25 mg/cm 2 ). • Immobilized cellulase retains 70 % activity after 8 reuse cycles. • LB-immobilized cellulase enables SSF with 0.6 g/L ethanol and 8-cycle reusability. By providing a flexible microenvironment, immobilization of cellulase on polymer brushes represents an effective strategy to improve the interactions of cellulase with the substrate in biomass conversion. However, manipulation of polymer brush morphology remains largely unexplored, owing to the lack of an effective synthesis strategy. Herein, we developed a versatile visible-light-induced graft polymerization strategy to precisely fabricate three types of polymer brushes with well-defined topologies on low-cost low-density polyethylene (LDPE) films. Benefiting from its unique three-dimensional high-density topology, linear-bottlebrush (LB) architecture exhibited a unique 3D high–density topology and achieved the highest enzyme loading capacity (0.25 mg/cm 2 ) and favorable enzymatic activity. The reusability was also demonstrated with 70 % of its initial activity remained after 8 reuse cycles for LB architecture. Furthermore, potential of LB brush-based immobilized cellulase in simultaneous saccharification and fermentation (SSF) of corncob for bioethanol production was demonstrated with its efficiency and reusability in practical biomass conversion processes. This study provides a synthetic methodology for constructing complex biointerfaces and establishes clear structure–property relationships, offering design principles for high-performance immobilized enzyme systems and advancing sustainable biomanufacturing. Download: Download high-res image (145KB) Download: Download full-size image
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2026
·
IF 4.4
Chen Zhou,Anxin Lin,Tenghui Tang,Min Zhang,Longqiang Xiao,Rui Zhao,Linxi Hou
A series of fluoroalkyl-functionalized and polymerizable photoinitiators were designed by integrating interfacial enrichment and covalent anchoring into a single molecular platform. • Fluoroalkyl segments induce spontaneous interfacial enrichment, thereby effectively enhancing resistance to oxygen inhibition during UV photopolymerization. • Polymerizable double bonds enable covalent incorporation into polymer networks, significantly suppressing post-curing migration of residual photoinitiators. • A structure, property, and kinetics relationship dependent on fluorine content was established, providing design principles for high-performance photoinitiators. UV photopolymerization is widely used in coatings, 3D printing, and biomedical materials due to its high efficiency and low energy consumption. However, Irgacure 2959, a representative Type I photoinitiator, suffers from oxygen inhibition and migration of residual species, limiting its practical applications. Herein, Irgacure 2959 was used as a molecular scaffold and structurally modified to construct a series of fluoroalkyl-functionalized polymerizable derivatives (2959-IA-nF, n = 0–5) by introducing vinyl polymerizable groups and tunable fluoroalkyl segments, enabling coupled interfacial transport and covalent immobilization. The effects of fluorine content on photolysis behavior, interfacial migration, oxygen tolerance, polymerization kinetics, and extraction stability were systematically investigated. All fluorinated derivatives exhibit higher photolysis rates than Irgacure 2959. Driven by reduced surface energy, 2959-IA-nF spontaneously migrates to the gas-liquid interface, with increasing migration flux as fluorine content rises, thereby mitigating oxygen inhibition. Polymerization kinetics show a volcano-type dependence on fluorine content, with 2959-IA-3F exhibiting the highest apparent rate constant (0.1209 s −1 ), approximately threefold that of 2959. Vinyl groups enable covalent incorporation into the polymer network, reducing extraction in acetonitrile from 21.43% to <1.5% and decreasing aqueous UV absorbance by over one order of magnitude. Density functional theory reveals lower excitation free energy for 2959-IA-3F, facilitating intersystem crossing and efficient photolysis despite a higher cleavage barrier. The cured films exhibit reduced yellowing, tunable hydrophobicity, and improved elongation at break. This work demonstrates a coupled interfacial transport-reaction strategy for oxygen-tolerant, low-migration photoinitiators. Download: Download high-res image (159KB) Download: Download full-size image
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高影响因子
CONSTRUCTION AND BUILDING MATERIALS
·
2025
·
IF 8
Yu Huang,Jiangnan Peng,Mingyan Qin,Wen Xiong,Chao Wang,Yaxin Qin,Wei Liu
The valorization of construction solid wastes into functional materials presents a dual opportunity for environmental remediation and sustainable infrastructure development. Herein, we successfully repurposed asphalt-based solid waste into calcium-enriched carbonaceous adsorbent through sequential pyrolysis (C Asp ) and ball-milling (C Asp, bm ) techniques for 4 h at a constant rotational speed of 550 rpm. It was noteworthy that both the C Asp and C Asp, bm demonstrated an ultrafast adsorption efficiency for Cd(II), achieving equilibrium within 5 min. The maximum adsorption capacity (207.54 mg/g) was obtained through pyrolysis at 900 ℃ followed by ball-milling (C Asp, bm -900). The adsorption process closely followed the pseudo-second-order kinetic model and Freundlich isotherm, characterized by an ultrafast adsorption phase succeeded by a slower equilibrium stage. The adsorption process was spontaneous and involved multilayer adsorption, primarily governed by chemisorption mechanisms. The analysis of ions species and characterizations revealed that both pyrolysis and ball-milling processes enhanced the removal efficiency of Cd(II) by altering the surface composition and functional groups, specific surface area, and surface defects of C Asp and C Asp, bm . The primary mechanisms for Cd(II) adsorption on C Asp, bm -900 included ion exchange with Ca(II), precipitation in conjunction with CO 3 2- , and electrostatic attraction. Unfortunately, the presence of Cu(II) and Pb(II) significantly inhibited the removal of Cd(II), attributed to their markedly higher electronegativity. This study proposes a promising strategy for fabricating cost-effective and high-efficiency adsorbents through the upcycling of waste asphalt into functional materials, thereby enabling the effective removal of heavy metals from wastewater.
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Yanbin Xiong,Junnan Cui,Tong Lou,Pan Cao
Biofouling due to marine microorganism adhesion poses significant challenges for marine vessels, necessitating innovative antifouling solutions. This study aimed to investigate the antifouling performance of an antimicrobial peptides (AMPs) immobilized on dopamine-modified stainless steel (SS) surfaces, as a novel approach for antifouling applications. Herein, dopamine was utilized to form a modification layer on SS, subsequently immersed in AMPs solution to create SS-DA-A samples. The surface characteristics were characterized through X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FESEM), contact angle measurement instrument, fourier transform infrared spectroscopy (FTIR), and confocal laser scanning microscopy (CLSM). Antimicrobial properties were assessed using plate counting, measuring bacterial adhesion, and optical density value, while algal resistance was determined through SEM and CLSM methods, specifically assessing the coverage by algae. The results demonstrated the successful modification of the SS surface, characterized by FTIR peaks corresponding to AMPs, and a significant reduction in contact angle with the introduction of dopamine. Notably, the SS-DA-A surface exhibited enhanced antimicrobial efficacy against Vibrio natriegens ( V. natriegens ), Staphylococcus aureus ( S. aureus ), and Escherichia coli ( E. coli ), with a marked reduction in biofilm formation, and C. pyrenoidosa and P. tricornutum coverage decreased by approximately 96.46 % and 91.61 %, respectively. Molecular dynamics simulation results reveal that antimicrobial peptides interact with the bacterial phospholipid bilayer, disrupting bacterial integrity and thus achieving surface antimicrobial activity. This study effectively demonstrates that dopamine facilitates the successful immobilization of AMPs on SS surfaces, leading to significantly improved antibacterial, anti-algal, and biofilm resistance properties, and offers a promising new strategy for antifouling technology in marine settings.
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高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2025
·Vol. 334
·期 2
·1287-1297
·
IF 1.6
Li Yifan,Cao Shuang,Yang Qingming,Wang Yuannuo,Wang Jingyang,Jiao Caishan,Zhang Meng,Zhang Lei
The removal of Cs and Sr from high level radioactive waste is very important for ecological protection. However, simultaneous immobilization of Cs and Sr is rarely reported. In this work, a perovskite structure CsSr 2 Ta 3 O 10 was synthesized by molten salt method. CsSr 2 Ta 3 O 10 has good thermal stability up to 900 °C at least. Under the γ irradiation of Co-60, most of the material maintained its structural integrity. CsSr 2 Ta 3 O 10 demonstrates a wide range of pH durability, where Cs is stable in the pH range from 4 to 12 and Sr from 2 to 12. Its frame structure remains stable in the pH range from 2 to 12 and will not be decomposed. Further sintering of CsSr 2 Ta 3 O 10 ceramic waste forms results in a high density of 93% − 95%. Moreover, leaching experiments conclude that the long-term leaching rates of Cs and Sr begin to stabilize after 14 days, reaching the order of 10 0 and 10 −3 g·m −2 ·d −1 . These results provide a possibility for the simultaneous immobilization of Cs and Sr.
研究领域:
材料科学
·
环境科学
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高影响因子
危险品化学品经营许可证（带存储）