APPLIED CLAY SCIENCE
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2019
·
IF 3.89
Ke Wang,Shiyong Sun,Biaobiao Ma,Faqin Dong,Tingting Huo,Xujuan Li,Yulian Zhao,Haili Yu,Yang Huang
Clay minerals provide an excellent platform for enzyme immobilization , which can improve enzyme activity and stability for industrial applications. In this study, lipase from Aspergillus oryzae was immobilized on 3-aminopropyltriethoxysilane (APTES) amphiphilic functionalized montmorillonite (Mt) support via 1-(3-Dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (EDC) spacer. The APTES-Mt and APTES-Mt based nanostructured biocatalyst (lipase-Mt) were characterized by XRD, FT-IR, TEM, SEM and contact angle analysis. Optimal immobilization conditions were determined considering parameters of EDC concentration, initial lipase concentration, pH, and reaction time. The results indicated that lipase-Mt activity was 40.65 U/mg, which was nearly 4-fold higher than that of free lipase under optimal conditions. The Michaelis-Menten constant ( K m ) were found as 0.357 mM and 3.406 mM for free and lipase-Mt, respectively. The maximum reaction rate ( V max ) for the free and lipase-Mt were calculated as 63.69 mM/(L·min) and 312.5 mM/(L·min), respectively. Further, the interfacial activation by amphiphilic surface of APTES-Mt and enlarged catalytic interface contributed to the improved activity and storage stability of lipase-Mt. Thus, this work demonstrate an economically viable method for constructing nanostructured biocatalyst for industrial applications based on covalent immobilization of enzyme onto clay minerals.
研究领域:
酶工程
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纳米生物技术
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工业生物催化
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胶体与界面化学
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应用微生物学
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DOI
高影响因子
Molecular Catalysis
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2022
·
IF 5.089
Bin Zou,Feng Liu,Yan Yan,Ting Feng,Josephine Naa Norkor Dowuona
In this study, coupling with substrate ultrasonic pretreatment technology, magnetic cross-linked enzyme aggregates (CRL-FIL-CLEAs@Fe 3 O 4 ) was used as novel biocatalyst for efficient preparation of phytosterol oleate in solvent-free reaction system. The CRL loading was 0.16 mg protein/mg CRL-FIL- CLEAs@Fe 3 O 4 . The effects of ultrasonic parameters, catalyst dosage, substrate molar ratio, and reaction temperature on the synthesis of phytosterol esters were studied. The results showed that the catalytical properties of CRL-FIL-CLEAs@Fe 3 O 4 (7.09±0.11 µmol phytosterol/mg protein. hour) was significantly improved compared with natural lipase from Candida rugosa (CRL, 5.13±0.21 µmol phytosterol/mg protein. hour). Simultaneously, ultrasonic pretreatment greatly enhanced substrate mass transfer, further reducing the esterification time. The max conversion rate 94.72% of phytosterol was obtained after 9 h reaction under optimum condition (the molar ratio of oleic acid to phytosterol was 6:1, the concentration of CRL-FIL-CLEAs@Fe 3 O 4 was 10 wt% of the phytosterol mass). After five recycles, the activity of CRL-FIL-CLEAs@Fe 3 O 4 (80.02%) was still 2.68 times higher than that of natural lipase system (29.91%). Meanwhile, the novel immobilized lipase (CRL-FIL-CLEAs@Fe 3 O 4 ) before and after reaction was characterized by SEM, AFM, VSM, TEM, and TGA. The results showed that CRL-FIL-CLEAs@Fe 3 O 4 was a robust and recyclable biocatalyst for phytosterol esterification.
研究领域:
生物催化
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酶工程
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食品科学
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DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2026
·
IF 5.1
Yi Zhao,Jinhong Wang,Shuang Yu,Xinlan Zhuo,Xiang Li,Zhiyi Chen,Guizhao Liang
Pancreatic lipase (PL) is a validated peripheral target for limiting dietary fat absorption, yet structurally diverse natural inhibitors remain scarce. We assembled a PL inhibitor dataset from public resources and trained a random-forest classifier using PubChem fingerprints (test AUC = 0.9134) to prioritize a natural product library. After drug-likeness and toxicity filtering, docking, and experimental validation, three hits were identified: Licochalcone C (IC 50 = 62.05 ± 1.77 μM), Neoglycyrol (IC 50 = 95.21 ± 0.57 μM), and Notopterol (IC 50 = 104.27 ± 3.19 μM). Interaction fingerprint and molecular dynamics analyses showed that binding was dominated by hydrophobic interactions, with Val260/Ala261 acting as key residues across the three PL-ligand complexes. Dissociation free-energy profiles from steered molecular dynamics and umbrella sampling were consistent with the potency ranking. Collectively, this data-driven pipeline identified new natural PL inhibitors and provided residue-level insights for further optimization.
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 44
·20316-20321
·
IF 3.3
Yong Tang,Kaifu Zhang,Yuelin Xu,Jinglin Ma,Hanqing Xie,Hongquan Zhang,Yanmin Jiang,Rui Zhao,Lei Wang
A green and efficient method for the construction of functionalized 2,3-dihydrothiophene derivatives in water was reported. In this work, Novozym 435 acted as a remarkable biocatalyst in catalyzing the four-component reaction of aldehydes, malonitrile or ethyl cyanoacetate, 1,3-thiazolidinedione, and amines under mild conditions. Excellent yields (85–95%) of all the corresponding products could be obtained in reasonable reaction time. Moreover, Novozym 435 could be reused for six cycles with a yield of at least 80%. The rationality of this multicomponent reaction mechanism was also verified via control experiments.
研究领域:
有机化学
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生物催化
·
绿色化学
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DOI
高影响因子
Catalysts
·
2019
·Vol. 9
·期 11
·914
·
IF 3.444
Huixia Yang,Weiwei Zhang
Enzymatic production of biodiesel from waste cooking oil (WCO) could contribute to resolving the problems of energy demand and environment pollutions.In the present work,Burkholderia cepacialipase (BCL) was activated by surfactant imprinting, and subsequently immobilized in magnetic cross-linked enzyme aggregates (mCLEAs) with hydroxyapatite coated magnetic nanoparticles (HAP-coated MNPs). The maximum hyperactivation of BCL mCLEAs was observed in the pretreatment of BCL with 0.1 mM Triton X-100. The optimized Triton-activated BCL mCLEAs was used as a highly active and robust biocatalyst for biodiesel production from WCO, exhibiting significant increase in biodiesel yield and tolerance to methanol. The results indicated that surfactant imprinting integrating mCLEAs could fix BCL in their active (open) form, experiencing a boost in activity and allowing biodiesel production performed in solvent without further addition of water. A maximal biodiesel yield of 98% was achieved under optimized conditions with molar ratio of methanol-to-WCO 7:1 in one-time addition in hexane at 40 °C. Therefore, the present study displays a versatile method for lipase immobilization and shows great practical latency in renewable biodiesel production.Keywords:biodiesel;waste cooking oil;lipase immobilization;interfacial activation;functionalized magnetic nanoparticles
研究领域:
生物化学
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环境科学
·
纳米技术
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DOI
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2016
·Vol. 92
·期 6
·1203-1210
·
IF 2.738
Fengli Wang,Guanglei Zhao,Xiongfei Lang,Junrong Li,Xiaofeng Li
BACKGROUND Long-chain cellulose esters can be used as packaging materials, film materials, biodegradable plastics and drug delivery agents. Chemical methods are commonly adopted for preparation of these esters using traditional chemical catalysts and organic solvents, which result in environmental and safety problems. RESULT A new enzymatic method was tested for synthesis of long-chain cellulose esters in mixed ionic liquids. The key reaction conditions, including mass ratio of ionic liquids, acylating agents, lipase/cellulose mass ratio, reaction temperature and substrate molar ratio, showed significant influences on enzymatic acylation. FT-IR and 1 H NMR spectra indicated the successful acylation of hydroxyl groups. SEM and XRD data showed significant changes in morphologies and crystallinity. The thermal properties of products were different from the original due to the disruption effect of ILs and the enzymatic acylation. CONCLUSION A facile one-step route is presented for synthesis of long-chain cellulose esters. The lipase-catalyzed synthesis is an ecofriendly and green approach for the modification of cellulose by replacing traditional chemical catalysts with enzymes and replacing organic solvents with ionic liquids. This biocatalytic approach may find its applications in preparation of potentially biodegradable materials for food, pharmaceutical and related industries. © 2016 Society of Chemical Industry
研究领域:
生物化学
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材料科学
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环境科学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
·
IF 6.1
Jian Ming,Yueru Sun,Yuanyuan Chen,Qiming Wang,Jinlong Li
According to the “interfacial activation” mechanism, constructing a sufficient interface is the key strategy for lipase-catalytic system designing. Based on the “infinite interface in finite three-dimensional space” logic, in the current study, poly(N,N-dimethylacrylamide) (PDMA)-polybutyl methacrylate (PBMA) hybrid gels were prepared by a two-step crosslinking strategy, subsequently constructed as lipase-interfacial catalytic systems. The results confirm that the PDMA-PBMA hybrid gels with “networks in pores” structures could swell both the aqueous phase and organic phase. The balance between water swelling and isooctane swelling, hybrid gel space (height control), and the lipase entry manner significantly affect the interface construction and consequently the catalytic efficiency. The enzyme–substrate contact rate affected by swelling leads to three catalytic stages. Considering the spatial barrier and distribution of lipases, a potential high-performance lipase reactor can be assembled from small-size, lamellar-like, and porous hybrid gels. The reactors also show good time storage and low temperature tolerance.
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Bingyong Mao,Weiling Guo,Zhouqun Huang,Xin Tang,Qiuxiang Zhang,Bo Yang,Jianxin Zhao,Shumao Cui,Hao Zhang
Walnut milk is rich in linoleic acid (LA) and linolenic acid (LNA) in the form of triglycerides. Four LAB strains capable of converting LA and LNA could grow to 9.0 lg CFU/mL in walnut milk. However, no conjugated fatty acids were detected. Lipase was added to release LA and LNA and then LAB was inoculated to ferment walnut milk. However, conjugated linoleic acid (CLA) and conjugated linolenic acid (CLNA) were only detected in walnut milk inoculated with Bifidobacterium breve CCFM683. When 60 U/mL lipase was added, the total amount of conjugated fatty acids reached 1.33 ± 0.01 mg/mL with conversion rates of 5.0% and 11.3% for CLA and CLNA, respectively. By monitoring the fermentation progress, the amount of conjugated fatty acids could reach a maximum of 2.30 ± 0.01 mg/mL at 20 h ( p < 0.05). When 20 U/mL lipase was added, the amount of conjugated fatty acids in fermented walnut milk reached 1.58 mg/mL and conversion rates increased to 7.2% and 21.6% for CLA and CLNA, respectively. Thus, adding lipase can realize the conversion of CLA and CLNA in walnut milk and reducing the amount of lipase and hydrolysis time is beneficial to improve the conversion rates, but total amount is reduced.
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产品关联
DOI
高影响因子
Catalysts
·
2022
·Vol. 12
·期 3
·339
·
IF 4.501
Li-Hua Du,Meng-Jie Yang,Yue Pan,Ling-Yan Zheng,Shi-Yi Zhang,Zhi-Kai Sheng,Ping-Feng Chen,Xi-Ping Luo
Coumarin carboxamide derivatives are important building blocks for organic synthesis and chemical biology due to their excellent biopharmaceutical properties. In this paper, we demonstrate for the first time a two-step enzymatic synthesis of coumarin carboxamide derivatives. Salicylaldehyde and dimethyl malonate were reacted to obtain coumarin carboxylate methyl derivatives, which were then reacted with various amines under the catalysis of lipase TL IM fromThermomyces lanuginosusto obtain coumarin carboxamide derivatives in continuous flow reactors. We studied various reaction parameters on the yields. The important features of this method include mild reaction conditions, a short reaction time (40 min), reduced environmental pollution, higher productivity (STY = 31.2941 g L−1h−1) and enzymes being relatively easy to obtain.Keywords:enzymatic synthesis;microreactor;coumarin carboxamide derivatives
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DOI
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2022
·
IF 7.419
Jie Mei,Ruilin Guo,Fan Zhang,Haiyan Zhang,Xinping Yang,Boyang Yu,Jihua Liu,Xiufeng Liu
Identification of bioactive principles from natural products is considered a challenging task in drug discovery. Recently, ligand fishing has been growing in interest as a sustainable strategy. In this study, a yeast-based drug discovery strategy was investigated to screen and fish active ingredients from natural products. Human monoacylglycerol lipase (MAGL) was first displayed on the cell wall of Pichia pastoris . The expression of MAGL on the cell surface was confirmed by immunofluorescence analysis. The activity toward 7-HCA which was consistent with free enzymes in solution. Recombinant yeast strains were used to screen the potential inhibitors from traditional Chinese medicines. Preliminary screening showed that the extracts of 12 herbs showed inhibition on MAGL activity, among which Corydalis Rhizoma had the highest inhibition rate of 60.66 ± 2.44%. Recombinant yeast fishing combined with HPLC-Q-TOF-MS/MS analysis was further used to identify the potential MAGL inhibitors. Finally, dehydrocorydaline (DHC) in Corydalis Rhizoma was determined as a ligand to MAGL with the IC 50 value at 154.7 μΜ. Corydalis Rhizoma has been used as a pain reliever in TCM. Intraperitoneal injection of 7 mg kg − 1 DHC in chronic constriction injury model rats significantly attenuated the mechanical allodynia and thermal hyperalgesia. Meanwhile, 2-arachidonoylglycerol, the major MAGL substrate in the brain, was significantly increased both in the hippocampus and striatum. In conclusion, yeast-based ligand fishing combined with HPLC-Q-TOF-MS/MS is a powerful strategy for drug discovery in complex mixtures and DHC from Corydalis Rhizoma was confirmed with high inhibitory activity to MAGL either in vitro or in vivo .
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Xue Shi,Xiaoli Qin,Yunxiang Dai,Xiong Liu,Weifei Wang,Jinfeng Zhong
The catalytic activity of Candida antarctica lipase B (CALB) immobilized on modified cellulose nanocrystals (CNC) with different hydrophobicity was investigated using experimental and theoretical approaches. Firstly, the modified CNC were characterized by multi-spectroscopic methods, water contact angle, scanning electron microscopy and thermogravimetric analysis. Moderately hydrophobic CNC were found to be an optimal support for CALB immobilization. Secondly, model systems contained a CALB molecule and different numbers of modified CNC molecules ( [email protected] , [email protected] and [email protected] ) were prepared for molecular dynamics (MD) simulation. Root-mean-square fluctuation values (0.61–2.61 Å) of lid region were relatively high in [email protected] , indicating that modified CNC with moderate hydrophobicity favored forming a lid-open conformation of CALB. Finally, the esterification of oleic acid catalyzed by the immobilized CALB showed higher conversion (54.68 %) than free CALB (12.98 %). Insights into modified CNC with tunable properties provided by this study may be a potential support for improving the catalytic performance of lipases.
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2021
·
IF 3.757
Yangyang Cai,Shuqi Xing,Qifeng Zhang,Ruonan Zhu,Kai Cheng,Cuiqin Li,Xuefeng Zeng,Laping He
The extracellular Aspergillus niger GZUF36 lipase (EXANL1) has broad applicability such as synthesis of functional lipids. The wild-type strain is unstable, and the culture time is long. Studies on the selectivity of substrates for EXANL1 are not available. Therefore, this study aimed to express, purify, characterize and analyze the substrate specificity of EAXNL1. The results showed that EXANL1 was stable in the presence of 40 % ( v/v ) dimethyl sulfoxide, glycerol, toluene, and n-hexane. The relative activity was 104 %, 88 %, 81 %, and 76 %. The residual enzyme activity of EXANL1 was more than 85 % in surfactants with a low concentration. EXANL1 exhibited a high conversion rate (79 %) for p -nitrophenyl palmitate. The molecular docking of p -nitrophenyl palmitate into the active site of EXANL1 indicated that the preference for this fatty acid chain length might be due to the formation of hydrogen bonds. The distance between the carbonyl carbon atom of p -nitrophenyl palmitate and the nucleophilic oxygen atom of serine 162 was 2.91 Å. Both experiments revealed that EXANL1 exhibited higher specificity for p -nitrophenyl palmitate. These results indicated that EXANL1 could be a promising tool in organic synthesis and detergent industry. The molecular docking provided a basis for the explanation on the substrate selectivity of EXANL1.
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DOI
高影响因子
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