Discover Oncology
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2025
·Vol. 17
·期 1
·1
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IF 2.9
Li Mengyan,Dai Litao,Jiang Min,Lv Yuanyuan,Zhang Yahao,Wang Yuqi,Ma Yuqing
Objective All-trans retinoic acid (ATRA) has demonstrated great potential in cancer treatment; however, the molecular mechanism for the anti‐tumour effects of Cellular retinoic acid-binding protein 2 (CRABP2) and ATRA in oesophageal squamous cell carcinoma (ESCC) remains unclear. Methods A strategy of transfecting KYSE-150 cells with vectors or CRABP2 overexpression was applied in this study. Changes in cell proliferation, migration, invasion and apoptosis in OE-NC, OE-CRABP2, OE-NC + ATRA and OE-CRABP2 + ATRA groups were observed. The location of CRABP2 with ATRA and the expression of key proteins that may be involved were detected. The volume and weight of subcutaneous grafted tumours in nude mice were compared in vivo, cell morphology was observed through haematoxylin and eosin (HE) staining, and cell apoptosis was detected using TUNEL staining. Results We determined that, in vitro, CRABP2 combined with ATRA best inhibited the proliferation, migration, invasion and cell cycle of ESCC and promoted its apoptosis. Oxidative stress, the mitochondrial pathway, p53 and the NF-κB pathway may be involved in the synergistic inhibitory effect of CRABP2 and ATRA. In vivo, tumour volume in the OE-CRABP2 + ATRA group was the smallest and tumour growth was the slowest. The HE results demonstrated improved cell differentiation in the OE-CRAB2 + ATRA group, and TUNEL staining revealed that CRABP2 and ATRA had the strongest promoting effect on cell apoptosis, with the degree of cell apoptosis the greatest with the participation of ATRA. Conclusion The combination of CRABP2 overexpression and ATRA synergistically inhibits ESCC progression. All‐trans retinoic acid may be a candidate therapy for ESCC, and CRABP2 overexpression could enhance the efficacy of ATRA treatment.
研究领域:
肿瘤学
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分子生物学
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癌症研究
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细胞生物学
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PubMed
高影响因子
Journal of Materials Chemistry A
·
2025
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IF 9.5
Faxiu Zhang,Jing Xie,Xinxin Yin,Gaoyuan Liu,Zhenjiang Lu,Jindou Hu,Yong Wen,Yali Cao
Despite the high capacity of metal selenides (MSes) anode materials for sodium-ion batteries (SIBs), they still face significant challenges, including rapid capacity degradation and slow reaction kinetics. Herein, a novel structure of Bi2Se3 nanospheres uniformly embedded in a nitrogen-doped carbon nanofiber matrix (denoted as Bi2Se3−x@CNFs) was constructed through a facile fabrication approach involving electrospinning, followed by one-step selenization, demonstrating exceptional performance as an SIB anode. The extremely high mechanical strength and consecutive fibrous structure of carbon fibers effectively alleviate the agglomeration and volume expansion of Bi2Se3 particles and ultimately improve stability and service life. Furthermore, the engineered selenium vacancy distribution in both Bi2Se3 nanospheres and carbon fiber substrates produces synergistic effects, significantly improves bulk electronic conductivity, and expedites redox reaction kinetics. As expected, the Bi2Se3−x@CNFs-600 electrode demonstrates high capacity and exceptional durability (327.8 mAh g−1 after 1000 cycles at 3.0 A g−1 and 284.0 mAh g−1 over 3000 cycles at 5.0 A g−1). It is noteworthy that the assembled Bi2Se3−x@CNFs//Na3V2(PO4)3 full cells demonstrate good rate capability and stability, rendering them a viable option for SIBs.
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高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2015
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IF 2.587
Yujia Xie,Fengyong Zhang,Pingle Liu,Fang Hao,Hean Luo
Direct functionalization of saturated C–H bonds by metalloporphyrin catalysts is among the topical and challenging areas within the chemical industry and synthetic chemistry. In this work, supported CoD(p-Cl)PPCl metalloporphyrin catalysts were prepared and characterized. It has been found that the CoD(p-Cl)PPCl/ZnO catalyst shows better catalytic activity than the supported catalyst (ZrO2, MCM-41, kaolin, Zr(OH)2, and boehmite (BM)) in cyclohexane oxidation with dioxygen. The effects of the reaction pressure, reaction temperature, and reaction time on the catalytic activity were considered by the supported CoD(p-Cl)PPCl/ZnO catalyst. The CoD(p-Cl)PPCl/ZnO catalyst can be facilely recovered and was recycled up to seven times without a significant decrease in catalytic performance. The average cyclohexane conversion and selectivity to KA oil are 10.98% and 84.34%, respectively, and the turnover number is 2.10 × 107.
研究领域:
催化化学
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有机合成
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工业化学
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材料化学
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绿色化学
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DOI
高影响因子
Journal of Materials Chemistry C
·
2019
·Vol. 7
·期 35
·11022-11028
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IF 6.641
Ji-Xing Gao,Zhong-Xia Wang,Yuan-Yuan Tang,Yu-Ling Liu,Lin Zhou,Xiao-Gang Chen,Wan-Ying Zhang,Ren-Gen Xiong
Ferroelectric polarization is sensitive to external stimuli, such as temperature or pressure, the variation of which can induce a slight change in crystal structure. Similarly, for ferroelectric crystals in host–guest systems, the inclusion of different guest molecules can affect the crystal packing a small amount; however, it may alter the ferroelectric properties significantly. In this report, we discovered that the supramolecular host–guest system Cu(1,10-phen)2SeO4·(diol) (phen is the abbreviation of phenanthroline) can distinguish cis–trans isomers via physical signal feedback, i.e. dielectric/ferroelectric responses. The supramolecular crystal is nonferroelectric and shows inconspicuous dielectric behavior when trans-1,2-cyclohexanediol is included, while a ferroelectric system is achieved when the host interacts with cis-1,2-cyclohexanediol. Moreover, the ferroelectric–paraelectric phase transition involved in cis-1,2-cyclohexanediol occurs together with an interesting chair-boat conformation change, as well as an order–disorder transition. As far as we know, this is the first discovery showing that dielectric/ferroelectric signal responses can be used to distinguish cis–trans isomers in the field of ferroelectric materials. This finding enriches studies of the molecular ferroelectric family and might provide inspiration for smart device design.
研究领域:
材料科学
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超分子化学
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铁电学
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晶体工程
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智能材料
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DOI
高影响因子
JOURNAL OF FOOD ENGINEERING
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2018
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IF 3.197
Zhaoliang Wu,Na Li,Xin Zhang,Yanyan Xu,Ting Shu,Wei Liu,Bin Hu
Resveratrol (2,5,4′-trihydroxystilbene) is a naturally occurring phytoalexin produced by some spermatogphytes and it has recently attracted the increasing attention of agronomists and pharmacologists owing to its satisfactory bioactivities. The objective of this work was to develop a cost-effective technology of foam fractionation to recover trans -resveratrol ( trans -Res) from the leaching solution of muscat grape pomace on the assistance of molecular imprinting modified SiO 2 nanoparticles . First, trans -Res was leached from muscat grape pomace through the method of alkaline extraction. Under the suitable leaching conditions, the leaching yield of trans -Res reached 84.34%. Adsorption experiments were performed for describing the adsorption behavior of trans -Res on the molecular imprinting modified SiO 2 nanoparticles . Results indicated that their adsorption could be fairly described by Freundlich model and fitted well with the pseudo-second-order kinetics model. Moreover, the molecular imprinting modified SiO 2 nanoparticles could effectively maintain the stability of trans -Res through avoiding the cis-trans photo-isomerization and oxidative degradation. Finally, foam fractionation was carried out for recovering trans -Res from the leaching solution, in which the concentration of trans -Res was as high as 29.09 mg/L. The molecular imprinting modified SiO 2 nanoparticles were used as the collector. Under the suitable conditions of loading liquid volume 250 mL, particle size of molecular imprinting modified SiO 2 nanoparticles 436.0 nm, sapindus saponin concentration 0.075 g/L, volumetric gas flow rate 100 mL/min and pore diameter of gas distributor 0.180 mm, the enrichment ratio and the recovery percentage of trans -Res reached 13.68 ± 1.03 and 89.73 ± 3.57%, respectively. This work is expected to provide a new technology for selectively recovering non-surface-active materials from the solution with their isomers or analogues, and to facilitate the industrialization of foam fractionation.
研究领域:
食品科学
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生物化学
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药理学
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DOI
高影响因子
FOOD CHEMISTRY
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2026
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IF 9.8
Yi Sun,Bao Chen,Haobo Jin,Sadaqat Ali,Yuanyuan Zhang,Xiaohui Lv,Xiang Fan,Guofeng Jin,Long Sheng
This study investigated the interaction between trans-cinnamaldehyde (TC) and ovalbumin (OVA) at the molecular level and its impact on the gel properties of OVA. Multispectral analysis, molecular docking, and molecular dynamics simulations were employed to elucidate the underlying mechanisms. At room temperature, TC bound to OVA mainly through non-covalent interactions, characterized by static quenching, which consequently altered the secondary structure of OVA. Moderate TC incorporation significantly enhanced the textural properties of thermally induced OVA gels, with hardness, chewiness, and gumminess increased by 39.4%, 28.0%, and 34.9%, respectively (P < 0.05 ). In addition, TC treatment improved the gel's water-holding capacity (by 4.38%), optimized water distribution, and promoted a more uniform microstructure. These improvements were primarily attributed to TC-induced Schiff base formation and non-covalent interactions, which collectively modulated intermolecular forces within OVA. Overall, this study provides novel insights into the use of spice-derived compounds to enhance the structure and functional quality of protein-based foods.
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PubMed
高影响因子
Journal of Analytical Methods in Chemistry
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2022
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IF 2.594
Ying Xu,Song-xia Zhang,Jing Guo,Li-jie Chen,Yu-ligh Liou,Tai Rao,Jing-bo Peng,Ying Guo,Wei-hua Huang,Zhi-rong Tan,Dong-sheng Ou-yang,Hong-hao Zhou,Wei Zhang,Yao Chen
Mice are the most frequently used animals in pharmacokinetic studies; however, collecting series of blood samples from mice is difficult because of their small sizes and tiny vessels. In addition, due to the small sample size, it is problematic to perform high required quantification. Thus, present work aims to find an effective strategy for overcoming these challenges using trans-resveratrol as a tool drug. Based on the idea of a joint technology, the capillary microsampling (CMS) was chosen for blood sample collection from mice after delivery of trans-resveratrol (150 mg/kg) by gavage, and a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the determination of trans-resveratrol and its main metabolites. All the mouse blood samples were exactly collected by CMS without obvious deviation. This provided credible samples for subsequent quantitative analysis. The HPLC-MS/MS method was found to be sensitive, accurate, and repeatable, and the pharmacokinetic parameters for all analytes were comparable with those reported in previous studies. However, the present joint technology offers the advantages of less animal damage, easy for sample preparation, and improved reliability. It has overcome some of the major limitations revealed in previous pharmacokinetic studies in mice and therefore provides a more effective option for future studies.
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PubMed
高影响因子
ORGANOMETALLICS
·
2022
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IF 3.837
Jixing Wang,Suting Xu,Xiang Hu,Yanchen Huo,Xiaochao Shi
Rare-earth metal complexes bearing pyridyl-functionalized amidinate ligands were synthesized, and upon activation by a borate, all of these complexes exhibited high catalytic activities for the living polymerization of isoprene and myrcene to generate trans-1,4 polymers (≤96.8% for polyisoprene and ≤94.8% for polymyrcene). A metal dependence effect was observed, and the scandium complexes with a smaller central ion afforded polymers with the highest trans-1,4-regularity. Moreover, the 3-Sc/[PhMe2NH][B(C6F5)4] binary system also promoted block copolymerization of isoprene and ε-caprolactone, affording the copolymers that consisted of a nonpolar polyisoprene block possessing trans-1,4-microstructure and a polar poly(ε-caprolactone) block.
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高影响因子
JOURNAL OF MOLECULAR STRUCTURE
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2022
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IF 3.841
Meng-Yao Wu,Fan-Zhi Bu,Yu Yu,Yue-Ming Yu,Zhi-Yong Wu,Cui-Wei Yan,Yan-Tuan Li
To highlight the preponderance of excipients maleic acid (MA) and fumaric acid (FA) in perfecting physicochemical peculiarities of antifungal drug econazole (ENZ), furthermore get some innovative cognitions on the regulation of solid crystalline form and antifungal activity of ENZ through regioisomers of unsaturated aliphatic dicarboxylic acids, two novice crystalline complexes of ENZ involving one cocrystal with FA, ENZ-FA ( 1 ), and one molecular salt with MA, ENZ-MA ( 2 ), are gained and structurally identified. The single-crystal X-ray diffraction analysis confirms that the component ratios of 2:1 and 1:1 for ENZ and corresponding acid molecules severally in 1 and 2 . Both the cocrystallized solids have sandwich layer structures with hydrophobic surfaces and hydrophilic inner supramolecular networks with varied supramolecular aggregates, resulting in the simultaneous increasement in their dissolubility and permeability, thereinto, 1 owns higher permeability, while 2 is more ascendent in dissolubility, which are demonstrated by the combination of theory with experiment approaches. Specifically, compared with parent drug ENZ, the optimized physicochemical properties have endowed these cocrystallized complexes with strengthened in vitro antifungal activities, displaying larger inhibition rings and smaller MIC values, comparatively, 2 showcases stronger antifungal ability than that of 1 . All the outcomes supply different molecular crystalline solids for ENZ, and fill the gap of its cocrystal investigations.
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高影响因子
EXPERIMENTAL EYE RESEARCH
·
2021
·
IF 3.467
Lei Tao,Danxue He,Chunyan Liao,Binxiang Cai,Chao Chen,Yan Wang,Jingmeng Chen,Zuguo Liu,Yalin Wu
Retinal pigment epithelium (RPE) cell apoptosis arising from all- trans -retinal (atRAL) is in close contact with the etiology of dry age-related macular degeneration (AMD) and autosomal recessive Stargardt's disease (STGD1), but its underlying mechanisms remain elusive. In this study, we reported that c-Jun N -terminal kinase (JNK) activation facilitated atRAL-induced apoptosis of RPE cells. Reactive oxygen species production and endoplasmic reticulum stress were identified as two of major upstream events responsible for activating JNK signaling in atRAL-loaded RPE cells. Inhibiting JNK signaling rescued RPE cells from apoptosis induced by atRAL through attenuating caspase-3 activation leading to poly-ADP-ribose polymerase (PARP) cleavage, and DNA damage response . Abca4 −/− Rdh8 −/− mice upon light exposure exhibit rapidly increased accumulation of atRAL in the retina , and display severe RPE degeneration, a primary attribute of dry AMD and STGD1. Reducing JNK signaling by intraperitoneally injected JNK–IN-8 was highly effective in preventing RPE atrophy and apoptosis in light-exposed Abca4 −/− Rdh8 −/− mice. These findings afford a further understanding for contribution of JNK activation by atRAL to retinal damage .
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Xianfeng Wang,Xiaolong Chen,Chengxiang Chu,Yuanyi Deng,Mei Yang,Zhong Ji,Faliang Xu,Danqun Huo,Yang Luo,Changjun Hou
High-performance detection of adenosine triphosphate (ATP) is of great importance due to its irreplaceable roles in various physiological processes. In this work, a four-stage signal amplification was established for ATP detection based on an allosteric probe-conjugated strand displacement amplification (SDA) integrated with CRISPR/Cpf1 system (ASD-Cpf1). An allosteric probe (AH) with a hairpin structure was designed to reduce background interference from self-extension and non-specific amplification during the SDA reaction. ATP bound with the aptamer segment at 5′-terminal of AH probe and subsequently opened the AH probe to activate the SDA reaction. The amplification products were recognized by CRISPR/Cpf1 system and triggered the ssDNase activity of Cpf1, resulting in robust cleavage of surrounding DNA FQ-reporter (fluorophore-quencher labeled short single-stranded DNA sequence). With the four-step amplification, ATP was measured with high sensitivity and low background interference. Under the optimal conditions, a linear relationship was obtained between the fluorescence response and ATP concentrations ranging from 2 pM to 10 μM with a limit of detection (LOD) as low as 1.8 pM. Successful detection of ATP in diluted human serum samples further indicated that ASD-Cpf1 system provided a potential platform for detection of small molecules.
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高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Xiaojiao Chen,Junhu He,Guiyu Tan,Ju Liang,Yuxia Hou,Mian Wang,Baomin Wang
trans -Resveratrol, a natural polyphenol present in grapes, has many beneficial effects to human health. However, measurement of trans -resveratrol is technically complicated and time-consuming. In the present study, we obtained a sensitive and specific monoclonal antibody (mAb), namely 3C9, against trans -resveratrol. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed, with 50% inhibitory concentration and working range of 1.0 ng/mL and 0.19–4.9 ng/mL, respectively. A lateral flow immunoassay (LFIA) based on 3C9 was also developed for the semiquantitative detection of trans -resveratrol in an indicator range of 50–100 ng/mL. Average recoveries of trans -resveratrol spiked in red and green grape berries samples were respectively 88–107% and 83–102% by icELISA. The icELISA and LFIA were applied for determination of trans -resveratrol in grape berries. The results were highly consistent with those determined by high-performance liquid chromatography analysis (R 2 = 0.9997). Therefore, we conclude that the immunoassay methods are suitable for the large-scale screening of trans -resveratrol in grape quality breeding.
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PubMed
高影响因子
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