PLANT GROWTH REGULATION
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2025
·
IF 3.9
Ling Qingyan,Zhang Beihong,Xiao Zufei,Shen Yao,Jiang Junfei,Xu Zhiwei,Liu Qingqing,Xiao Changlong,Zeng Rong,Liu Yuanqiu,Jin Zhinong
Citral-type Camphora officinarum ( C. officinarum ) exhibits high economic value due to its branch and leaf essential oil enriched with citral, yet the low natural concentration of this compound necessitates urgent strategies to enhance yield and quality. Methyl jasmonate (MeJA), a recognized safe elicitor, stimulates aromatic compound accumulation in plants and promotes monoterpene synthesis in C. officinarum suspension cells. However, it remained unclear whether foliar MeJA application enhances monoterpene biosynthesis in C. officinarum . This study evaluated the effects of near-harvest foliar MeJA application at gradient concentrations 0 μmol/L (control, M0), 100 μmol/L (M100), 250 μmol/L (M250), and 500 μmol/L (M500) on growth, oil gland morphology, and essential oil composition in citral-type C. officinarum . The results demonstrated that: M100 treatment significantly increased plant height, branch biomass, leaf biomass, and total biomass compared to the control M0. M250 treatment induced structural modifications in oil cells, enlarging their diameter and increasing density, resulting in an 11.83% increase in essential oil yield per unit fresh leaf weight. Both M100 and M250 treatments enhanced citral content by 3.6% and 3.31%, respectively, while total essential oil production surged by 12.31% (M100) and 15.46% (M250). Principal component analysis (PCA) collectively demonstrated that 100–250 μmol/L MeJA (M100/M250) represented the optimal induction concentration for enhancing essential oil yield and promoting citral synthesis, whereas 500 μmol/L (M500) induced detrimental osmotic stress. These findings demonstrate that MeJA serves as a safe agricultural regulation approach for enhancing essential oil yield and citral content, offering valuable insights for other aromatic plant species.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2025
·Vol. 36
·期 22
·1-15
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IF 2.8
Zhou Hengqing,Li Xiao,Gao Guoqi,Sun Huajun,Liu Xiaofang
BiOBr/PVDF composite films with different precursor doping amounts were successfully prepared by in situ deposition phase conversion technology. The physicochemical properties of the composite films were analyzed in detail, and their piezoelectric-photocatalytic performance was systematically investigated. The experimental results showed that BP-0.8 (with a doping amount of 0.8 mmol for the BiOBr precursor) exhibited the best catalytic performance. Under the synergistic effect of visible light irradiation and ultrasonic vibration, the degradation rate of BP-0.8 for 50 mL of 40 mg/L Rhodamine B (Rh B) solution within 60 min could reach 96.73%, and the apparent reaction rate constant (k) of this sample was 0.06198 min⁻ 1 . It was 3.81 times and 27.06 times that of photocatalysis alone and piezoelectric catalysis alone, respectively. The radical capture experiment proved that the main active groups involved in the reaction were h⁺ and ·OH, and the mechanism of piezoelectric photocatalysis of BiOBr/PVDF composite thin films was proposed. In addition, compared with powder catalysts, flexible BiOBr/PVDF membranes were easier to recycle, did not require complex treatment, and still maintained good catalytic activity after ten cycles of testing. This work demonstrated the contribution of the piezoelectric effect to photocatalytic water purification and provided a promising strategy to utilize solar and mechanical energy.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-13
·
IF 15.7
Liu Shenning,Wang Yuxian,Liu Ya,Chen Peihan,Kong Tao,Duan Xiaoguang,Chen Chunmao,Sun Hongqi,Wang Shaobin
Cobalt-containing spinel oxides are promising platforms to fine-tune the intrinsic activity/selectivity of their geometric sites in catalysis. However, the role of tetrahedrally occupied Co 2+ (Co 2+ Td ) and Co 3+ in an octahedral site (Co 3+ Oh ) in controlling the catalytic activity remains controversial. Herein, we investigated a geometrical-site-dependent catalytic activation of ozone respectively on the Co 2+ Td and Co 3+ Oh sites. The same exposure of [111] crystal facet is achieved by substituting those undesired sites with catalytically inactive cations. The highly spin-polarized Co 2+ Td sites invoke strong orbital interactions and intensive electron transfer with the adsorbed O 3 and become the active sites for selectively producing surface-bound hydroxyl radicals ( • OH) and avoiding the formation of unfavorable singlet oxygen ( 1 O 2 ), resulting in a 17.6-fold increase in turnover frequency (TOF). This work enlightens the spin-polarized electronic states into regulating the reaction thermodynamics in transition metal oxide-induced catalysis and envisages the practical application potentials of geometric site engineered spinel oxides.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-10
·
IF 15.7
Li Jingpeng,Zhang Meiyu,Li Wenxuan,Li Zhongshu,Zhu Tingshun,Yang Zhenyu
Silicon is a cornerstone material in electronics and photovoltaics due to its abundance, tunable semiconducting properties, and chemical versatility. Direct anchoring of thiophenes, with their highly delocalized aromatic backbones, onto silicon surfaces offers a promising route to tailor charge carrier migration properties. However, current methods for anchoring thiophenes commonly rely on pre-activation of precursors or transition-metal catalysts. Here, we introduce a catalyst-free radical strategy for direct linkage of thiophenes with Si atoms on organosilanes and silicon surfaces. This method leverages thermally induced homolytic cleavage of Si-H bonds to generate silicon radicals, which undergo efficient hydrosilylation with thiophene rings, forming Si-C linkages and releasing H 2 . We demonstrate the successful application of this approach on silicon surfaces, achieving functionalization with thiophenes that enhance charge carrier mobilities in silicon nanocrystals significantly higher than previously reported alkyl-functionalized SiNCs, indicating the significant potential of catalyst-free dehydrocoupling for advancing silicon-based materials in optoelectronic applications.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-21
·
IF 15.7
Zhang Hugang,Jia Jiaxin,Liu Hanyu,Han Haobo,Li Quanshun
Rheumatoid arthritis is a common autoimmune disease characterized by chronic synovial inflammation and joint destruction, primarily driven by an imbalanced cellular metabolism and inflammatory microenvironment. While gene therapy offers a promising therapeutic approach, its effectiveness is limited by the challenges of non-specific gene expression in healthy tissues. Here, we develop a gene delivery system (namely APPC), in which near-infrared (NIR)-responsive gold nanorods are coated with chondroitin sulfate-modified polyethyleneimine to facilitate the heat-responsive targeted delivery of heme oxygenase 1 (HO-1) gene. The APPC shows favorable transfection efficiency due to its targeting ability and significantly facilitates HO-1 expression under NIR irradiation. The combination of APPC/pHO-1 and NIR can effectively reprogram the cellular metabolism and repolarize the macrophages and fibroblast-like synoviocytes, thereby inhibiting inflammation by suppressing glycolysis. Meanwhile, APPC can specifically enhance the HO-1 expression in inflamed tissues through NIR-mediated the activation of heat shock protein 70 promoter, ensuring the precise gene expression via photothermal conversion. In a collagen-induced arthritis model, APPC/pHO-1 under NIR irradiation exhibits potent therapeutic efficacy, restoring the articular microenvironmental homeostasis and mitigating the symptoms of rheumatoid arthritis. These findings highlight the potential of APPC/pHO-1 nanoparticles in the gene therapy of rheumatoid arthritis and other inflammatory diseases.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 9
·1-12
·
IF 5.3
Chen Rui,Pang Chaohai,Ma Xionghui,Wu Xuejin,Liu Chunhua,Yin Guihao,Zhang Zhenshan,Li Shuhuai
An ECL signal amplification strategy is introduced leveraging a multimetallic catalytic amplification effect. Nickel–iron bimetallic oxide compounds were synthesised and loaded with Cu SAs catalysts on their surface to create nickel–iron layered double hydroxides/copper single atoms (NiFe-LDHs/Cu-SAs) with robust catalytic properties. These NiFe-LDHs/Cu-SAs were employed as signal amplification elements to modify gold electrodes. C-dots labelled with thiacloprid DNA aptamers were used as the signal and molecular recognition elements to construct an electrochemiluminescence (ECL) immunosensor. The NiFe-LDHs/Cu-SAs significantly amplified the ECL signal of the C-dots, enhancing the sensitivity of the sensor. Upon capturing thiamethoxam via the aptamer, the ECL signal was effectively quenched, demonstrating the potential of this approach as a highly sensitive method for thiamethoxam detection. The excellent catalytic performance of NiFe-LDHs/Cu-SAs amplified the detection signal over 20-fold, while the aptamer improved the specificity of the sensor for thiamethoxam recognition. The method demonstrates a linear range of 2–6000 × 10 −12 mol/L and a detection limit of 6.52 × 10 −13 mol/L, with recoveries of 90%–110% for real sample analysis. This study demonstrates a highly sensitive and specific method for thiamethoxam detection, with real-world applicability, while highlighting an innovative multimetallic catalytic strategy that can facilitate advancements in sensor design across various fields. Graphical
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 9
·1-12
·
IF 5.3
Xiang Tianxin,Wen Jin,Fang Jianhua,Nie Chuang,Cheng Na,Ying Ying,Tan Hongliang
Enzymatic assays represent a promising alternative to conventional multiplex optical methods for multi-target detection due to their exceptional specificity and catalytic versatility. Nevertheless, most existing enzymatic systems are limited to single-target detection. In this work, we developed a dual-signal multiplex assay based on non-tandem enzymes for the simultaneous detection of multiple pathogenic bacteria. This assay utilizes the distinct catalytic activities of horseradish peroxidase (HRP) and invertase (INV) to produce two orthogonal signal outputs. Specifically, HRP catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine to produce a blue chromogenic signal measurable by absorbance, while INV hydrolyzes sucrose to yield glucose, which is quantified with a personal glucose meter. To implement this assay, HRP and INV are individually encapsulated within zeolitic imidazolate framework-90, and Fe 3 O 4 nanoparticles functionalized with phenylboronic acid are employed for dual-recognition of target pathogens. By integrating spatiotemporal control of enzymatic reactions with distinct signal separation, the assay effectively minimizes crosstalk and enhances detection accuracy and reliability. This assay demonstrates excellent sensitivity and selectivity in milk samples, highlighting its potential for practical applications. We believe this work establishes a versatile and robust platform for multiplex pathogen detection, with significant implications for food safety, clinical diagnostics, and environmental monitoring. Graphical
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Communications Biology
·
2025
·Vol. 8
·期 1
·1-13
·
IF 5.1
Liang Congying,Lin Lu,Wang Xiaoyan,Zhu Wenping
The secretion of ligninolytic enzyme provides a competitive advantage for microbial survival. These enzymes are commonly transported to the extracellular milieu via signal peptides for the catabolism of lignin, which cannot be translocated through the cell membrane. However, some bacterial ligninolytic enzymes lack signal peptides, yet they can still be secreted. It remains unclear how these unconventional proteins cross the cell membrane. Here, we reveal a secretion mechanism for the unconventional B-type dye-decolorizing peroxidase (DypB) in Pseudomonas putida A514. Type VI secretion system (T6SS) mediates the inner membrane channel, where interaction of DypB with the T6SS components VgrG and Hcp accounts for periplasmic delivery. Once in the periplasm, DypB is allocated into outer membrane vesicles (OMVs) and released to the extracellular space. The crosslinked translocation model for DypB delivery represents an ingenious mechanism by which bacteria harness extant systems to thrive in a nutrient-poor environment. Moreover, we develop an OMV-surface display platform to improve DypB secretion and further enhance lignin utilization, exemplifying the applicability of OMVs as a lignin biocatalytic nanoreactor. Our study demonstrates the previously unrecognized crosslinking of T6SS and OMVs for the secretion of unconventional ligninolytic peroxidase and provides new perspectives on its biotechnological applications.
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VETERINARY RESEARCH COMMUNICATIONS
·
2025
·Vol. 49
·期 5
·1-10
·
IF 2
Liu Shuang,Qiu Bo,Li Yue,Wang Hai-Peng,Zhong Zhao-Jin,Wang Lin
Glyphosate (Gly) is a globally prevalent herbicide, which poses significant health risks to human and animals via the food chain. The kidney is the primary excretory organ and a site of residual accumulation during Gly exposure. Trehalose (Tre) has been shown to prevent kidney injury induced by various toxicants due to its anti-apoptotic properties. However, the potential protective effect of Tre on Gly-induced kidney damage in poultry remains unclear. Thus, 60 one-day-old healthy Hy-line Brown roosters were randomly divided into three groups ( n = 20), i.e., control group (basal diet without Gly), Gly group (200 mg Gly/kg basal diet), and Gly + Tre group {(200 mg Gly plus 5 g Tre)/kg basal diet}. The experiment lasted for 120 days, and serum and kidney samples were finally collected for respective analysis. Data first showed that Tre treatment can significantly alleviate Gly-induced renal injury in roosters, as evidenced by serum levels of creatinine and blood urea nitrogen as well as renal histopathological observations. Tre also significantly inhibited the elevated abundance of renal injury marker proteins KIM-1 and NGAL induced by Gly. Meanwhile, Tre obviously alleviated Gly-induced ultrastructural abnormalities in the nuclei of renal tissues. Additionally, Tre markedly inhibited Gly-induced apoptotic assays in rooster kidneys, as demonstrated by the results from TUNEL staining and the protein abundance of apoptotic markers cleaved caspase-3 and cleaved PARP. In summary, these findings suggest that Tre exerts its protective effects against Gly-induced kidney damage in roosters via inhibiting apoptosis.
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MRS Advances
·
2025
·
IF 0.9
Wang Xiaodong,Yu Xinrun,Xu Yushan,Yu Juan,Huang Pei
A series of PI aerogel composite films were prepared through adjustments made to the ratio of dianhydride and chemical imidization reagents, the crosslinker content, and the utilization of a non-solvent induced phase separation process. The prepared PI aerogel films exhibited a minimum dielectric constant of 1.39 (10 6 Hz), a maximum water contact angle of 137.8°, and demonstrated excellent recyclability. Furthermore, the prepared polyimide aerogel films demonstrated exceptional thermally stable characteristics, with Td 5% maintained at approximately 510 °C, Tg values remaining above 230 °C, and a low coefficient of thermal expansion, at 55.8 ppm/K. The prepared PI aerogel films exhibit a high degree of porosity (74.1%), a relatively low density (0.392 g/cm 3 ), and considerable tensile strength (up to 6.2 MPa). It has been demonstrated that these polyimide aerogel films are highly promising for the construction of high-frequency microelectronic devices. Graphical abstract
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NATURE MATERIALS
·
2025
·
IF 38.5
Wang Meng,Cao Fengren,Meng Linxing,Wang Min,Li Liang
The extension of optoelectronic devices from planar to non-developable structures has led to remarkable success in bionics, optical imaging and soft electronics. However, non-developable optoelectronic devices are achieved mainly via physical deformations and limited to a few geometries. Here we report a self-assembly perovskite strategy for integrating optoelectronic arrays with arbitrary non-developable structures. The perovskite films are grown from a rapid nucleation-dominated crystallization driven by the low energy fluctuation of lead iodide solution, where the fluid precursor can be evenly dispersed along non-developable substrates by surface tension and then self-assembles into compact films through gaseous manipulation. The strategy covers arbitrarily shaped substrates with three-dimensional length scales over 10 6 orders of magnitude and enables the unique structural manipulations of photodiode arrays with micrometre precision. As a proof of concept, the theoretical focal surface of a single-lens image system is realized into a non-developable sensor, effectively correcting the off-axis coma aberrations compared with its planar or hemispherical counterpart.
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CURRENT MICROBIOLOGY
·
2025
·Vol. 82
·期 9
·1-11
·
IF 2.6
Gu Yiwen,Lin Xiu,Lu Yanxu,Fang Shuguang,Hu Kun,Ju Xin,Li Liangzhi,Chen Zhi
The increasing use of antibiotics in shrimp aquaculture has raised concerns regarding ecological safety and the emergence of antibiotic resistance. This study aimed to isolate and characterize probiotic lactic acid bacteria (LAB) strains from the gut of Penaeus vannamei to evaluate their potential as antibiotic alternatives in aquaculture. Two strains, S1 and S2, were identified with 99.79 and 99.51% similarity to Pediococcus acidilactici DSM 20284 and Enterococcus lactis BT159, respectively. Based on minimum inhibitory concentration (MIC) assays, both strains exhibited high sensitivity to the tested antibiotics. They also demonstrated notable acid and bile salt tolerance, with S1 maintaining a survival rate of 30% at pH 5 and S2 achieving 40% survival at pH 6. Under 0.03% bile salt concentration, survival rates were approximately 60% (S1) and 45% (S2), while S1 retained 15% viability even at 0.9% bile salt. Enzymatic activity analysis indicated that both strains exhibited lower amylase activity compared to other hydrolytic enzymes, with S2 showing higher cellulase and protease activities than S1. Antimicrobial assays revealed that both strains exerted broad-spectrum inhibition against four indicator pathogens. S1 exhibited the strongest inhibitory effect against Aeromonas hydrophila (inhibition zone: 1.6–2.2 cm), whereas S2 showed the highest inhibition against Vibrio parahaemolyticus (1.5–2.4 cm). Feeding trials demonstrated that the combined application of S1 and S2 significantly enhanced shrimp body weight (6.05 ± 0.09 g vs. 9.23 ± 0.2 g, P < 0.01) and survival rate (62.49% ± 1.56 vs. 93.78% ± 2.55, P < 0.01). These findings highlight the potential of Lactobacillus strains as probiotics in shrimp farming, offering a sustainable approach to reducing antibiotic dependency and improving shrimp health and productivity.
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