Molecular Catalysis
·
2022
·
IF 5.089
Yuefeng Deng,Yihui Zhu,Zhenni Jiao,Ziqian Chen,Jianjun Wang,Chengli Yang,Dali Li,Kefeng Ma,Ruofu Shi
Coenzymes are required in the reaction catalyzed by oxidoreductases. The research on coenzyme regeneration has always been a hot topic in the field of biological catalysis. In this work, we tried to construct a co-immobilization system of glucose dehydrogenase (GDH), ( R )-1-phenylethanol dehydrogenase (( R )-PEDH) and coenzyme with functional magnetic nanoparticles as carriers, so as to realize chiral ( R )-1-phenylethanol highly selective production and coenzyme effective recycling. The enzymatic properties of immobilized enzymes were explored. The results showed that the immobilized enzymes had improved stability and fine reusability. In addition, during the catalytic coupling reaction at a final concentration of 2.63 μM NAD + , the total turnover number (TTN) was as high as 924, and the volumetric productivity of ( R )-1-phenylethanol was 37.06 mg·L −1 ·h −1 . After reused for 10 cycles, the catalytic efficiency of immobilized coenzyme can still be maintained at more than 30%. From multiple perspectives, the immobilized dual-enzymes and coenzyme system seems to be a multifunctional system for ( R )-1-phenylethanol production and coenzyme regeneration.
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高影响因子
Annals of Translational Medicine
·
2022
·Vol. 10
·期 18
·971-971
·
IF 3.616
Shuqi Zhao,Meifeng Chen,Zhu Yu,Thi Thai Hoa Pham,Shutian Mo,Yongfei He,Tianyi Liang,Wenlong Cao,Chuangye Han
BackgroundBreast cancer (BC) is the most common type of cancer affecting females. It is also a leading cause of cancer-related death in women worldwide.MethodsSonodynamic therapy (SDT) is an emerging therapeutic strategy for cancer treatment. SDT ensures non-invasive penetration of deep tumors and results in activation of non-toxic sonosensitizers administered in deep tumor sites to become cytotoxic. It has been reported that 2-dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione (DMDD) has a significant anti-tumor effect against various cancer types including BC. However, DMDD is hydrophobic. Therefore, a one-step encapsulation method was used in the current study to construct zeolitic imidazole frameworks-8 (ZIF-8) loaded with DMDD and sonosensitizer chlorin e6 (Ce6). ZIF-8 was further modified by coating it with a biomimetic cell membrane to improve targeted delivery.ResultsIn vitro and in vivo results indicated that the nanomedicines had great biocompatibility properties and targeting ability. The nanocomposite exhibited a higher release rate under an acidic tumor microenvironment. The tumor killing effect of reactive oxygen species (ROS) generated from Ce6 and inhibition of tumor growth was enhanced after ultrasound (US) treatment, which might be caused by the increase in apoptosis rate.ConclusionsThese findings show that the combination of nanomedicine and SDT provides a potential therapeutic method for BC.Keywords: Breast cancer (BC), 2-dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione (DMDD), metal-organic framework, sonodynamic therapy, ZIF-8 nanoparticles
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PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2019
·
IF 3.218
Huang Chen,Peng Sun,Meijia Qiu,Min Jiang,Jie Zhao,Dongxue Han,Li Niu,Guofeng Cui
In response to the challenges posed by diabetes, portable glucose sensors with enzyme-free catalytic materials have received great attention in recent years. Herein, a facile strategy for fabricating inexpensive and efficient electrochemical sensors is proposed. Flexible copper clad laminate (FCCL) can be firstly decorated with nanoporous copper (NP Cu) through electrodepositing Cu-Sn alloy and dealloying the Sn element. Then Co-P is further electrodeposited onto the NP Cu/FCCL and the final Co-P/NP Cu/FCCL composite electrode exhibits high catalytic activity for glucose. The great performance of the Co-P/NP Cu/FCCL mainly derives from the enlarged surface area of the NP Cu and the high electrocatalytic activity of Co-P. This electrode exhibits a high sensitivity of 1818 μA cm −2 mM −1 , low limit of detection of 378 nM (S/N = 3), fast response time of 4 s, a wide linear dynamic range of 0.5 μM to 10.0 mM, good anti-interference performance, excellent repeatability and considerable stability for glucose detection. The well-designed structure for catalyzing glucose in high efficiency enable the Co-P/NP Cu/FCCL a potential candidate for future flexible electrochemical sensors.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
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2020
·Vol. 55
·期 22
·9525-9537
·
IF 3.553
Bai Ting,Zhu Bobo,Du Zhaoyi,Shi Junling,Shao Dongyan,Kong Jie
The combination therapy by using two or more chemotherapeutic agents with different action mechanisms has shown to be an attractive strategy for cancer treatment with synergistic therapeutic effects, and also the side effects reduction by one agent replacing part of another one. In this work, an amphiphilic star copolymer with an H40 hyperbranched polyester as the core and poly(ε-caprolactone)-poly(2-dimethylaminoethylmethacrylate) copolymer incorporated with the fluorescent DTM groups as the arms were designed to encapsulate the model anti-cancer drug, doxorubicin (DOX), and a novel potential agent, avasimibe (Ava). Those two drugs could inhibit DNA replication and block acyl-CoA cholesterol acyltransferase-1 (ACAT-1) to induce the cell apoptosis, respectively. The model drugs could be easily loaded into the micelles with a high total drug-loading content toward 24.6% by altering the feeding drug ratios. Three molar ratios (DOX:Ava = 3:1, 1:1, and 1:3) of the drug-loaded micelles were prepared and evaluated for in vitro antitumor efficacy. Results showed that the micelles with 1:1 ratio exhibited the best synergistic cytotoxic activity against both K562 and HeLa cells. Furthermore, the dual-drug combination with efficient anticancer performance was also confirmed by live/dead staining, apoptosis, and cell cycle analysis. Moreover, the internalization of these dual-drug-loaded micelles could be monitored by fluorescent DTM groups and DOX. All these results suggested that the addition of Ava could cut down the dose of DOX to reduce its potential side effects and the co-delivery system could be a promising approach for an effective combination chemotherapy.
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高影响因子
Acta Biomaterialia
·
2022
·
IF 10.633
Yang Wang,Danni Jing,Jiawen Yang,Shajun Zhu,Jian Shi,Xiru Qin,Wujie Yin,Jin Wang,Yue Ding,Tingting Chen,Bing Lu,Yong Yao
Carbon monoxide (CO) as one of the therapeutic gaseous molecules has been widely applied for treating various diseases, especially in cancer therapy. However, the in situ-triggered and efficient transport of CO to tumors are the primary obstacles that limit its clinical applicability. To address this obstacle, herein, a H 2 O 2 -triggered CO gas releasing nanoplatform has been designed by embedding manganese carbonyl (MnCO) into Zr (IV)-based metal-organic frameworks (MOFs). The porous structures of MOFs provide encapsulation capacity for glucose oxidase (GOx) loading, thereby catalyzing the endogenous glucose into gluconic acid and H 2 O 2 to accelerate CO release and energy depletion. In the meantime, the Mn 2+ produced by MnCO can react with intracellular H 2 O 2 via the Fenton reaction to form cytotoxic •OH. Therefore, the synthesized gas nanogenerator demonstrated a synergistic efficacy of CO gas therapy, reactive oxygen species (ROS)-mediated therapy, and energy starvation to prevent tumor growth. Both in vitro and in vivo studies indicated that this multifunctional nanoplatform not only successfully inhibited tumors through a synergistic effect, but also provided a new technique for the creation of starvation/gas/chemodynamic combination therapy in a single material. Statement of significance In this study, we developed a H 2 O 2 responsive CO gas nanogenerator to augment the in-situ generation of CO gas for combined modality therapy of tumors. The nanogenerator was constructed by encapsulating glucose oxidase (GOx) and manganese carbonyl (MnCO) into UiO-67-bpy, which can catalyze the conversion of intracellular glucose to H 2 O 2 for cutting off energy supply of cancer cells. Meanwhile, the cumulated H 2 O 2 can trigger the release of CO for gas therapy and generation of •OH for chemodynamic therapy (CDT) via the Fenton-like reaction, thereby resulting in apoptosis of the cancer cells. Collectively, our designed nanotherapeutic agent not only displays the synergistic therapy efficacy of starvation-enhanced CO gas therapy and CDT, but also provides an efficient strategy for developing the intelligent nanocarrier for CO gas delivery and release.
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PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 28
·15326-15335
·
IF 2.936
Chao Xu,Suchun Yu,Langlang Liu,Xiaopei Wu,Honglian Dai
A magnetically targeted drug delivery system (DDS) is developed to solve the delivery problem of hydrophobic drugs by using hollow mesoporous ferrite nanoparticles (HMFNs). The HMFNs are synthesized by a one-pot hydrothermal method based on the Ostwald ripening process. The biocompatibility of the synthesized HMFNs was determined by MTT assay, lactate dehydrogenase (LDH) leakage assay and hemolyticity against rabbit red blood cells. Moreover, Prussian blue staining and bio-TEM observations showed that the cell uptake of nanocarriers was in a dose and time-dependent manner, and the nanoparticles accumulate mostly in the cytoplasm. A typical highly hydrophobic anti-tuberculosis drug, rifampin (RFP) was loaded into HMFNs using supercritical carbon dioxide (SC-CO2) impregnation, and the drug loading amount reached as high as 18.25 wt%. In addition, HMFNs could co-encapsulate and co-deliver hydrophobic (RFP) and hydrophilic (isoniazide, INH) drugs simultaneously. The in vitro release tests demonstrated extra sustained co-release profiles of rifampicin and isoniazide from HMFNs. Based on this novel design strategy, the co-delivery of drugs in the same carrier enables a drug delivery system with efficient enhanced chemotherapeutic effect.
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高影响因子
ACS Biomaterials Science & Engineering
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2022
·
IF 5.395
Jidong Dai,Zhigang Zhang,Katrien V. Bernaerts,Qianli Zhang,Tianzhu Zhang
Common poly(lactide-co-glycolide) (i-PLGA) has emerged as a biodegradable and biocompatible material in tissue engineering. However, the poor hydrophilicity and elasticity of i-PLGA lead to its limited application in tissue engineering. To this end, an amphiphilic crosslinked four-armed poly(lactic-co-glycolide) was prepared. First, four-armed PLGA (4A-PLGA) was synthesized by polymerizing l-lactide (LA) and glycolide (GA) with pentaerythritol as the initiator. Then, the hydrophilic polymer poly(glutamate propylene ester) (PGPE) was prepared through the esterification of glutamic acid and 1,2-propanediol. The hydrophilic 4A-PLGA–PGPE was finally synthesized through the condensation reaction of 4A-PLGA and PGPE with the aid of triphosgene. 4A-PLGA–PGPE was then used to prepare amphiphilic membranes by electrospinning. It was demonstrated that the mechanical properties and biocompatibility of 4A-PLGA were improved after the introduction of PGPE. Furthermore, the introduction of glutamate improved the hydrophilicity of 4A-PLGA, thus effectively promoting cell entry and adhesion, which makes the electrospun 4A-PLGA–PGPE membranes promising for tissue engineering.
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PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2019
·
IF 14.229
Hongyu Du,Kai Wang,Panagiotis Tsiakaras,Pei Kang Shen
Noble metal electrocatalysts with excavated and dendritic architectures have recently attracted growing attention for their large accessible surface areas and high density of under-coordinated atoms. However, it is a tough challenge to avoid the atoms migrating to the corners and kinks and finally obtain the excavated structure, after the complicated interaction among multiple factors during the synthesis process, let alone the growth control of dendritic shape. Herein we demonstrate a facile strategy to synthesize Pt-Co nanocubes having excavated and dendritic structure (Pt-Co EDNCs). Specifically, the Pt-Co EDNC nanocrystal is composed of a deeply excavated nanocube and nanodendrites selectively protruding on the vertexes. Further electrocatalytic investigations showed that Pt-Co EDNCs exhibited enhanced catalytic properties towards the electro-oxidation of ethylene glycol and glycerol compared with commercial Pt/C, which could be attributed to both its unique structure and the synergism between Pt and Co.
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高影响因子
Oncotarget
·
2017
·
IF 5.168
Muhammad Sarfraz,Attia Afzal,Shahid Masood Raza,Sajid Bashir,Asadullah Madni,Muhammad Waseem Khan,Xiang Ma,Guangya Xiang
Doxorubicin in combination with other cytotoxic drugs has clinical advantages. However, doxorubicin-induced cardiotoxicity negatively impacts clinical utility and outcomes. Cardiotoxicity can result from increased oxidative stress or from a local cytochrome P450 mediated increase in 20-hydroxy-5, 8, 11, 14-eicosatetraenoic acid (20-HETE). Oleanolic acid (OA) is a natural pentacyclic triterpenoid with free radical scavenging, cardioprotective, and P450-mediated cyclooxygenase-upregulating properties. We investigated co-delivery of liposomal OA and doxorubicin in a HepG2 model of hepatocellular carcinoma (HCC). OA attenuated the cardiotoxicity induced by doxorubicin without compromising its anticancer activity. Apoptosis assays revealed that co-delivery of DOX and OA produced a synergistic anticancer effect. However, the drugs had antagonistic effects on cardiomyocytes. Female BALB/c nude mice treated with OA- and DOX-loaded liposomes (ODLs) exhibited reduced tumor growth, stable body weight, and stable organ indices. Reduced 20-HETE production suggested ODLs had limited cardiotoxicity. No changes in biochemical or histopathological markers were observed in mice treated with ODLs. Tailored co-delivery of OA and DOX may thus be an effective therapeutic strategy for treating HCC.
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DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2023
·
IF 10.7
Yang Guo,Zhi-guo Zhang,Jing Cai,Wei-rong Li,Ling-yun Chen,Wei-cheng Wu
The feasibility of co-folding of soy protein isolates (SPI) and shellac by pH-shifting method to induce a protein hydrogel was firstly investigated. SPI and shellac at different mass ratios (1.3, 1.5, 1.7, 2.1, and 2.5 w/w) were co-folded by adjusting pH back to 7, 8, and 9, after unfolding at pH 12. The pH-shifting treatments resulted in the conformational change of SPI and the formation of covalent bonds between SPI and shellac, as confirmed by sodium dodecyl sulfate−polyacrylamide gel electrophoresis (SDS-PAGE), Fourier-transform infrared (FTIR) spectroscopy, UV–Vis, fluorescence spectroscopy, and as well as the changes of thermogravimetry. The cold-set hydrogel was prepared from the pH-shifting process by adjust pH to 9 which showed the optimal hardness and rheological properties, accompanied by excellent water-holding capacity (WHC> 90%). Meanwhile, the microscopic observations revealed the formation of uniform microstructure in the gel network. The co-folded hydrogels were proved to have a lower degree of protein hydrolysis (DH) than the same pH-shifted SPI alone during in vitro simulated digestion. Thus, the simple pH manipulation of SPI and shellac may be applied to induce hydrogels, reducing the protein DH during in vitro digestion which provides the potential for delivery of sensitive nutrients.
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高影响因子
RENEWABLE ENERGY
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2022
·
IF 8.634
Naveed Ahmad,Ying Chen,Xiaoxiao Wang,Peixu Sun,Yuting Bao,Xia Xu
Electrochemical CO 2 reduction reaction (ECO 2 RR) offers an eco-friendly way to produce value-added products by utilizing the waste CO 2 from the environment. Recently, amines have been utilized as electrolytes for direct conversion of CO 2 but processes were energy-intensive and lack selectivity due to the formation of highly stable carbamate. 2-amino-2-methyl-1-propanol (AMP) is a sterically hindered amine , produces less stable carbamate, and generates more HCO 3 − . Herein, ECO 2 RR performance using AMP aqueous solution as electrolyte was investigated over Ag, Cu, and Zn metal electrodes. Ultimately, 91 ± 7% CO selectivity on HCl modified polycrystalline Ag electrode at −0.91 V vs RHE in 1 M AMP aqueous solution in the presence of CTAB was achieved, 1.7 times greater than in 1 M aqueous ethanolamine (MEA) solution. Synergetic effects of unstable AMP-carbamate, generation of nano-agglomerates on pitted/porous Ag surface arising from HCl treatment, and a compact electrical double layer due to CTAB present are responsible for the high selectivity of CO. Our findings provide the understanding of electrode-electrolyte interactions and offer a facile strategy to directly convert CO 2 to CO from CO 2 capture solutions.
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高影响因子
Frontiers of Chemical Science and Engineering
·
2020
·Vol. 15
·期 2
·340-350
·
IF 3.552
Zhang Wei,Xu Boren,Gong Caihong,Yi Chunwang,Zhang Shen
In this study, a facile and environmentally friendly method with low energy consumption for preparing nanoscale AgCl and BaSO 4 co-precipitates (AgCl@BaSO 4 co-precipitates) was developed based on the metathetical reaction. Then, the dried co-precipitates were melt-compounded with polyamide 6 (PA6) resins at a specified mass ratio in a twin-screw extruder. The results demonstrated that in the absence of any coating agent or carrier, the nanoparticles of AgCl@BaSO 4 co-precipitates were homogeneously dispersed in the PA6 matrix. Further analysis showed that after the addition of AgCl@BaSO 4 co-precipitates, the antibacterial performance, along with the flame-retardance and anti-dripping characteristics of PA6, was enhanced significantly. In addition, the PA6 composites possessed high spinnability in producing preoriented yarn.
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高影响因子
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