Nanoscale
·
2021
·Vol. 13
·期 45
·19109-19122
·
IF 7.79
Songbo Zhao,Jiazhi Duan,Yalin Lou,Ruyun Gao,Shanshan Yang,Piming Wang,Chunhua Wang,Lin Han,Minghuan Li,Chunhong Ma,Xiaohong Liang,Hong Liu,Yuanhua Sang,Lifen Gao
Although there has been significant progress in the development of tumor immunotherapies, many challenges still exist for the treatment of solid tumors. Natural killer (NK) cells possess broad-spectrum cytotoxicity against tumors, but their limited migration and infiltration abilities restrict their application in solid tumor therapies. Here, we combined a facile and efficient magnetic-targeting strategy with NK cell-based therapy to develop a novel immunotherapy approach for treating solid tumors. Anti-CD56 antibodies were conjugated with Fe3O4 nanoparticles, which could specifically bind with NK-92 cells endowing them with a magnetic field driven targeting ability. These NK–Fe3O4 biohybrid nanoparticles were able to facilitate directional migration to the tumor site in vivo under external magnetic field guidance and efficiently inhibit tumor growth. These functionalized NK cells represent a novel approach for solid tumor therapy and may provide a promising modality for cancer interventions in the future.
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高影响因子
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2025
·
IF 6.2
Qi Zhang,Shuangshuang Bai,Mingwan Liu,Zhuhua Gong,Yun Ren,Wenlin Zhang,Qiang Li,Qinhong Liao,Zexiong Chen,Jianmin Tang
Biomass-derived magnetic biochar is a promising imidacloprid adsorbent because of its low cost, easy separation, and eco-friendly features. This study prepared a novel magnetic Fe 3 C@Fe 3 O 4 core–shell biochar nanocomposite (Fe 3 C@Fe 3 O 4 /BC) using a facile one-step method with Zanthoxylum bungeanum pruning branches as raw materials and K 3 [Fe(C 2 O 4 ) 3 ]•3 H 2 O as the pore-forming agent and Fe source. The prepared Fe 3 C@Fe 3 O 4 /BC had a well-developed porous structure, a considerable specific surface area of 1471.3 m 2 g −1 , good magnetic separation performance, and excellent adsorption performance of imidacloprid. The adsorption capacity of Fe 3 C@Fe 3 O 4 /BC toward imidacloprid reached 288.9 mg g −1 within 1 min, and its maximum adsorption capacity was 557.1 mg g −1 . The adsorption performance of Fe 3 C@Fe 3 O 4 /BC was was stable under extreme environmental conditions, and its regeneration was excellent, which was superior to typical commercial adsorbents. The adsorption mechanism of imidacloprid by Fe 3 C@Fe 3 O 4 /BC involved the comprehensive effects of pore capture, van der Waals forces, hydrogen bonding, electrostatic attraction, and complexation of oxygen-containing functional groups. This work can provide an advanced strategy for the fabrication of high-performance adsorbents from biomass resources for the effective treatment of imidacloprid wastewater.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Kezhu Xu,Jin Wu,Qunling Fang,Linfeng Bai,Jinyu Duan,Jiaxing Li,Huajian Xu,Ailing Hui,Lingyun Hao,Shouhu Xuan
The organic pollutants and heavy metal ions are critical harmful substances in waste water because they destroy the ecological systems and lead diseases to animals and human beings. Unfortunately, the simultaneously remove of them in one system is still a challenge due to their different treating mechanism. Herein, a novel magnetic h-Fe 3 O 4 @Au/Polydopamine (Au/PDA) hybrid hollow nanosphere which can both absorb the potentially toxic U(VI) ions and catalyze the reduction of the 4-nitrophenol is reported. By using a simple in situ redox-oxidizing polymerization method, the h-Fe 3 O 4 hollow nanosphere is well encapsulated by the Au/PDA hybrid shell to form the dual-functional magnetic hollow nanocomposites. Owing to the hollow interior, uniform and functional PDA coating, and high active Au nanocrystals, the final h-Fe 3 O 4 @Au/PDA hollow nanosphere possesses high potential in nanocatalysis, heavy metal ions adsorption, and drug delivery. Because of the PDA protection, the h-Fe 3 O 4 @Au/PDA nanosphere can even keep 90% activity after 7 cycling catalytic reactions on reduction of 4-nitrophenol. Similarly, the h-Fe 3 O 4 @Au/PDA exhibits a good adsorption effect for U(VI) in nuclear waste and its adsorption capability reaches to 82.9 mg·g −1 by 6 successive adsorption–desorption experiments. Due to the facile magnetic separation, the h-Fe 3 O 4 @Au/PDA nanosphere can be easily collected by applying an external magnetic field. In summary, such a multifunctional recyclable h-Fe 3 O 4 @Au/PDA nanosphere with green and facile synthesis, easy manipulation, efficient adsorption performance and good catalytic activity possesses broad application potential in treatment of coexisting toxic water pollution.
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高影响因子
Journal of Biomedical Nanotechnology
·
2009
·Vol. 5
·期 5
·505-510
·
IF 0.989
Li Zhiyang,He Lei,Shi Zhiyang,Wang Hua,Li Song,Liu Hongna,Wang Zhifei,He Nongyue
This paper described that Fe3O4 nanoparticles were encapsulated with methyl methacrylate (MMA) using linolenic acid (LA) as a crosslinking agent, after which the resulting polymethyl methacrylate (PMMA) embed Fe3O4 nanoparticles (PMMA/Fe3O4) were coated with silica, forming SiO2/(PMMA/Fe3O4) core–shell structure particles. Then these magnetic nanoparticles (MNPs) were applied in the developed system of chemiluminescent magnetic enzyme-linked immunoassay. E. coli O157:H7 was sandwiched between rabbits anti-E. coli O157:H7 polyclonal antibody-coated magnetite nanoparticles (immunomagnetic nanoparticles or IMNPs) and mouse anti-E. coli O157:H7 monoclonal antibody (E. coli O157-McAb). Commercial alkaline phosphatase conjugated horse anti-mouse immunoglobulin (ALP-Ab) was used to bond with the monoclonal antibody, finally the chemiluminescent signals were detected by adding 3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane (AMPPD) which was the substrate reagent of ALP. The specificity and sensitivity of this system for detecting E. coli O157:H7 were researched. The results indicated that this method was of good specificity when using E. coli Top 10F′ and Vibrio cholera as negative controls. The detection limit was 103 cells mL−1 when the antigen solution was 1 mL, and the procedure duration was about 3 h.
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高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 40
·期 14
·2933-2940
·
IF 2.557
Ling-Feng Jiang,Bo-Cheng Chen,Ben Chen,Xue-Jian Li,Hai-Lin Liao,Wen-Yan Zhang,Lin Wu
The extraction adsorbent was fabricated by immobilizing the highly specific recognition and binding of aptamer onto the surface of Fe 3 O 4 magnetic nanoparticles, which not only acted as recognition elements to recognize and capture the target molecule berberine from the extract of Cortex phellodendri , but also could favor the rapid separation and purification of the bound berberine by using an external magnet. The developed solid-phase extraction method in this work was useful for the selective extraction and determination of berberine in Cortex phellodendri extracts. Various conditions such as the amount of aptamer-functionalized Fe 3 O 4 magnetic nanoparticles, extraction time, temperature, pH value, Mg 2+ concentration, elution time and solvent were optimized for the solid-phase extraction of berberine. Under optimal conditions, the purity of berberine extracted from Cortex phellodendri was as high as 98.7% compared with that of 4.85% in the extract, indicating that aptamer-functionalized Fe 3 O 4 magnetic nanoparticles-based solid-phase extraction method was very effective for berberine enrichment and separation from a complex herb extract. The applicability and reliability of the developed solid-phase extraction method were demonstrated by separating berberine from nine different concentrations of one Cortex phellodendri extract. The relative recoveries of the spiked solutions of all the samples were between 95.4 and 111.3%, with relative standard deviations ranging between 0.57 and 1.85%.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Mengqi Zheng,Hongjun Han,Jingxin Shi,Zhengwen Zhang,Wencheng Ma,Chunyan Xu
In current study, nano-Fe 3 O 4 @activated coke enhanced bio-system (FEBS) under limited-oxygen condition was applied for efficient treatment of aromatic organics in coal pyrolysis wastewater. Metagenomic analyses revealed functional microbiome linkages and mechanism involved in aromatic ring-cleavage. Based on biodegradation efficiency in different reactors, FEBS supplementation conferred the best organic removal (avg. 92.29%). It also showed a remarkable advantage in biodegradability maintenance (> 40%) over control reactors. Metagenomics profiling revealed the degradation processes were driven by Fe 3 O 4 redox reactions and microbial biofilm, while the suspended sludge was the principal force for aromatic mineralization . Based on the analysis of functional species and genes, most bacteria cleaved the benzene ring preferably through the aerobic pathways, mediated by catechol 1, 2-dioxygenase, catechol 2, 3-dioxygenase and protocatechuate 3, 4-dioxygenase (66−84%). Ecological network showed that Comamonas testosterone -centered microbiome and Azotobacter linked to the nitrogen (N)-heterocyclic ring-cleavage. Network linkage further demonstrated that Alicycliphilus and Acidovorax were the key tone taxa involved in benzene ring-cleavage. Finally, combined with analysis of degradation products , bacteria degraded N-heterocyclic ring containing organic aromatic compounds (quinoline) mainly through anaerobic processes , whereas cleavage of benzene ring preferred aerobic pathways. The enriched functional species were the primary reason for the enhanced biodegradation in FEBS.
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高影响因子
Journal of Zhejiang University-SCIENCE A
·
2015
·Vol. 16
·期 8
·669-679
·
IF 0.882
Shan Jie,Wang Li,Yu Hao-jie,Tai Yu-lei,Akram Muhammad
Porous polymers are very suitable materials for the adsorption of organic pollutants due to their abundant pores and organic frameworks in aqueous solution. However, their recovery from treated pollutant is difficult, and thus, their application is limited. A facile strategy to synthesize reusable magnetic porous microspheres (MPMS) of Fe 3 O 4 /poly(methylmethacrylate (MMA)- co -divinylbenzene (DVB)) is described in this paper. The magnetic microspheres were synthesized by suspension copolymerization. MMA was used as a monomer, DVB was used as a crosslinker, and the magnetic fluid was added to the organic phase. The morphology of MPMS was observed by scanning electron microscope (SEM) and other properties were tested by superconducting quantum interference device, Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), X-ray powder diffraction (XRD), and nitrogen sorption-desorption techniques. The synthesized MPMS possessed a high specific surface area using toluene as a porogen. It was further found that the ratio of DVB to MMA, the ratio of porogen to monomer, and the type of porogen all affected the specific surface area and the morphology of the microspheres. Furthermore, the microspheres were applied to remove Rhodamine B from its aqueous solution. The results showed that the microspheres possessed good adsorption capacity for Rhodamine B. This result was due to the porous structure, polar groups, and superparamagnetic characteristic of the synthesized microspheres.
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高影响因子
JOURNAL OF MATERIALS CHEMISTRY
·
2012
·Vol. 22
·期 40
·21426-21433
·
IF 5.968
Peng Zhang,Jia Guo,Changchun Wang
A novel multifunctional conjugated nanoporous polymer colloid (CNPC) combined with metal nanocrystals has been synthesized using the Sonogashira coupling reaction in a toluene-in-water miniemulsion. It is composed of a poly(phenylene ethynylene) (PPE) network that is covalently inlaid with the locked-in Fe3O4 nanocrystals. The Fe3O4@CNPC has a narrowly distributed size and uniform morphology, preserves the advanced porosity and fluorescence emission of the conjugated polymer network, and shows rapid magnetic response and high catalytic activity. The magnetic content can be controlled during the synthesis. This is accompanied by a corresponding enhancement of the size and surface area of the mesopores. In view of those characteristics, Fe3O4@CNPC is able to behave as fluorescence sensor for the detection of phenolic compounds. Acetaminophen (APAP) has been used as a model compound and the test results demonstrate that the Fe3O4-catalyzed oxidation of APAP takes place within the pores of the PPE network in the presence of H2O2. The oxidized intermediates are reactive radicals and can remove electrons from the π-conjugated PPE network, eventually leading to a decrease or even quenching of its fluorescence. Since the multiple functions of Fe3O4@CNPC fully cooperate, it produces a rapid optical response and superior sensitivity for the detection of APAP at micromolar concentrations.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Jiabi Xu,Yanyan Xing,Yutong Liu,Mingzhe Liu,Xiaohong Hou
In recent decades, artificial nanozymes with excellent stability, low cost and availability have been gradually explored to avoid the limits of natural enzymes such as poor stability, high cost and difficult preparation. Herein, for the first time, we investigated the capability of nanoscale Fe 3 O 4 @MIL-100(Fe) as a nanozyme, which was quickly synthesized in situ by a microwave-assisted method within 20 min using Fe 3 O 4 as the metal precursor. The obtained Fe 3 O 4 @MIL-100(Fe) showed satisfactory intrinsic dual enzyme mimetic activities, including peroxidase (POD)- and catalase (CAT)-like activities. Moreover, a simple and effective colorimetric biosensor was fabricated to detect glutathione (GSH) based on its POD-like activity. The proposed measurement had a linear range of 1–45 μM and a limit of detection (LOD) of 0.26 μM (3.3 δ/S). It was proved that the established colorimetric sensing system could be successfully applied to detect GSH in actual biological samples. Importantly, the outstanding reusability and stability made it extremely valuable as a catalyst. The present work implied that Fe 3 O 4 @MIL-100(Fe) synthesized in situ by the microwave-assisted method was a very promising candidate for biocatalyst and biosensing.
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高影响因子
CELLULOSE
·
2018
·Vol. 25
·期 8
·4565-4575
·
IF 3.809
Liu Kai,Chen Lihui,Huang Liulian,Ni Yonghao,Xu Zhenwei,Lin Shan,Wang Hongping
Nanocomposite hydrogels with conductive and magnetic properties can be used in a variety of new applications. However, the preparation of stable nanocomposite of conductive polymer and magnetic nanoparticles remains a significant challenge because of the aggregation of polymer and nanoparticles induced by the interparticle dipolar forces and electrostatic interactions. This research reported a strategy for the preparation of nanofibrillated cellulose/polypyrrole/Fe 3 O 4 nanocomposites using nanofibrillated cellulose as templates by the in situ chemical polymerization and chemical co-precipitation methods. Incorporation of the stable nanocomposites into agarose hydrogel resulted in conductivity and magnetism for the hydrogel. Moreover, the nanocomposite hydrogel exhibited reversible restorability in response to the thermal stimuli. The conductivity, magnetism, and reversible restorability make the nanocomposite agarose hydrogel a promising multi-functional material for some new applications such as electronic skin and electrochemical display devices. More importantly, the strategy is a useful method for preparing stable nanocomposites comprising conductive polymer and magnetic nanoparticles, which can impart different functionalities to hydrogels. Graphical abstract This research presents a facile strategy for preparing conductive and magnetic agarose nanocomposite hydrogels with reversible restorability.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2026
·
IF 3.9
Ma Kuanjie,Li Nana,Lu Guochao,Zhang Lianwei,Hu Zhiwei,Liu Wenjing,Cai Yurong
To address the need for rapid desorption in protein adsorption applications, we developed and characterized magnetic core–shell nanoparticles composed of a zeolite imidazolate framework-8 (ZIF-8) coating on magnetite (Fe 3 O 4 ) cores (Fe 3 O 4 @ZIF-8). This nanostructure integrates the pH-sensitive properties of ZIF-8 with the magnetic responsiveness of Fe 3 O 4 , enabling rapid separation within one minute via an external magnetic field. In the synthesis of Fe 3 O 4 @ZIF-8, the particle size of the ZIF-8 shell was found to increase concurrently with the molar ratio of zinc nitrate hexahydrate to 2-methylimidazole. The resulting shells were uniformly distributed on the surface of the Fe 3 O 4 cores. Furthermore, a positive correlation was observed between the ZIF-8 particle size and the adsorption capacity for histidine-rich proteins. In adsorption experiments using bovine hemoglobin (BHB) as a model protein, Fe 3 O 4 @ZIF-8 achieved an adsorption capacity of 1013.5 mg/g within 3 min, representing 87% of its maximum adsorption potential. Saturation adsorption, with a capacity of 1164.3 mg/g, was reached within 8 min. Importantly, the pH-responsive nature of the ZIF-8 component facilitated highly efficient desorption. A high release efficiency of 90% was achieved in just 1 min using a phosphate buffer at pH 5.2. This dual pH- and magnetic-responsive system demonstrates significant potential for applications requiring fast and controllable protein adsorption and desorption, positioning it as an ideal tool for targeted protein recovery in various biochemical and industrial processes.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Luoyuan Shi,Xinying Jiang,Shuang Sun,Chunping Jia,Xiaojun Bian,Jianlong Zhao
Rapid and reliable detection of foodborne pathogens is essential for ensuring food safety, yet achieving high selectivity and sensitivity in complex matrices remains challenging. Herein, we report a sensitive and selective electrochemical sensing platform integrating a bacteria-imprinted polymer (BIP) capture interface with concanavalin A-functionalized Fe 3 O 4 (Fe 3 O 4 -ConA) for the detection of Escherichia coli O157:H7 ( E. coli O157:H7). The BIP film was electropolymerized in situ on glassy carbon electrode to generate recognition sites for efficient capture of E. coli O157:H7. Subsequently, Fe 3 O 4 -ConA bound to surface glycans on the captured bacteria through lectin-glycan interactions, forming a sandwich-like sensing interface. The anchored Fe 3 O 4 nanozyme catalyzed the H 2 O 2 -mediated oxidation of o -phenylenediamine, producing amplified electrochemical signals for bacterial quantification. Benefiting from the combination of imprinting-based recognition and catalytic amplification, the sensor achieved an ultrasensitive detection limit of 2.8 CFU mL −1 and a linear range from 10 1 to 10 5 CFU mL −1 . Recoveries of 93.29–113.62% were obtained in spiked milk, fish, and juice samples, with reliable detection down to 10 CFU mL −1 in complex food matrices. This work provides an effective strategy for constructing nanozyme-amplified electrochemical sensors for sensitive and selective monitoring of foodborne pathogens.
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