LWT-FOOD SCIENCE AND TECHNOLOGY
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2020
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IF 4.006
Ting Liu,Jiamu Kang,Liu Liu
Pseudomonas aeruginosa is a notoriously difficult pathogen to control with antibiotics and disinfectants. Thymus vulgaris L. essential oil (TEO) and thymol are useful for replacing synthetic bacteriostatic agents due to their broad-spectrum bactericidal properties. This study focuses on understanding the inhibitory effects of TEO and its major component against P. aeruginosa , and exploring the antibacterial mechanism from the perspectives of cell membrane destruction, DNA intercalation and biofilm prevention. Results indicate that thymol is the principal component in TEO, as confirmed by GC-MS analysis. TEO and thymol potently combat the planktonic P. aeruginosa and directly change the cell structure. P. aeruginosa cell membrane integrity is destroyed as evidenced by an increase in permeability of the inner and outer membranes and the leakage of intracellular biological macromolecules (DNA). Our study further confirms that the accumulation of intracellular ROS caused by TEO and thymol can result in oxidative stress-mediated DNA damage. Thymol intercalates in the P. aeruginosa genomic DNA to affect the normal function of DNA and even cause bacterial death. Moreover, TEO and thymol affect the biofilm formation and limit the bacterial swimming and swarming motilities. This study provides a potential approach for the development and utilization of natural essential oils and bioactive compunds as novel food additives in the food processing.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Yang Luo,Yitao Zhang,Liqin Lu,Hongxia Luo
A graphene platform electrode (GPE) was fabricated by transferring chemical vapor deposition (CVD) graphene films from copper foils to flexible polyethylene terephthalate substrates supported by polymethyl-methacrylate. l -Aspartic acid ( l -Asp) was immobilized onto the surface of GPE by electrodeposition to form a poly- l -Asp/GPE, which was characterized by scanning electron microscopy, energy dispersive spectroscopy and Raman spectroscopy. Hence, a novel poly- l -Asp modified GPE was created for the simultaneous electrochemical determination of acetaminophen (AC), epinephrine (EP) and tyrosine (TY). Cyclic voltammetry was used for investigating the electrocatalytic activity of the poly- l -Asp/GPE sensor towards the detection of AC, EP and TY in comparison with that of GPE. The simultaneous determination of AC, EP and TY was realized by the differential pulse voltammetry technique at poly- l -Asp/GPE. The broad linear ranges to AC, EP and TY were obtained by chronoamperometry, which were from 0.05 to 108.25 μM for AC, 0.025 to 123.3 μM for EP and 0.1 to 93.9 μM for TY, with detection limits (S/N = 3) of 0.011 μM, 0.006 μM and 0.31 μM, respectively. Furthermore, the as-fabricated sensor showed extraordinary stability as well as high anti-interference ability, and was utilized successfully in detecting real samples.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Yun Liu,Zhina Qiao,Hengwei Zhang,Xian Zhang,Yanan Li,Zhiming Rao
D-tagatose, a functional rare sugar, has physiological effects such as lowering blood sugar and anti-caries. However, research on the industrial production of D-tagatose has primarily focused on Escherichia coli , limiting application in the food field. In this study, a cell factory for efficient synthesis of D-tagatose was constructed using the GRAS strain Corynebacterium glutamicum . Firstly, the key enzyme L-arabinose isomerase from Lactobacillus fermentum was screened. Secondly, by rational design, the optimal mutant D390V/H339P significantly enhanced enzyme activity by 40.65 % and improved thermostability. Subsequently, a dual-enzyme coupled whole-cell system was established by regulating the ratio of β-galactosidase and L-arabinose isomerase by RBS optimization. After optimizing the catalytic system, 101.42 g/L D-tagatose was produced from 400 g/L lactose, and the conversion rate reached 25.12 % in 5 L fermenter, which is the highest yield using lactose as substrate and GRAS strain as chassis cells. This research laid a foundation for the industrial application of food-grade D-tagatose.
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
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IF 5.1
Xinzong Li,Hongfeng Gao,Yujun Li,Yonggang Wang,Haiyong Zhang
A novel type of humic acid-based nitrogen-doped graphene quantum dots (N-GQDs) exhibiting strong blue fluorescence emission was synthesized using a straightforward hydrothermal method, with coal-derived humic acid and ethanolamine serving as precursors. It was observed that the incorporation of Fe 3+ ions quenches the fluorescence emission of the N-GQDs, the classification of quenching is recognized as static quenching; however, the addition of l -lysine to the N-GQDs/Fe 3+ system effectively restores this fluorescence. Building on these findings, this study further investigates the potential application of N-GQDs as an “on-off-on” fluorescent sensor for detecting both Fe 3+ and l -lysine. It has been established that N-GQDs exhibit commendable selectivity and sensitivity towards Fe 3+ , achieving a detection limit (LoD) of 0.41 μM. Furthermore, the stable N-GQDs/Fe 3+ complex demonstrates significant detection sensitivity to l -lysine in aqueous environments, with a LoD of 1.06 μM. The experimental results indicate that the synthesized N-GQDs possess favorable properties including excellent fluorescence intensity, dispersibility, and water solubility. The developed sensor exhibits considerable potential for improvement and holds promising applications in the domain of water quality monitoring. Additionally, the fluorescence selectivity of N-GQDs towards Fe 3+ was elucidated through calculations involving frontier molecular orbitals.
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Advanced Materials Technologies
·
2025
·
IF 6.2
Yi Xue,Zhaohua Liu,Ivan S. Babichuk,Yijie Liu,Jian Yang
Flexible pressure sensors have broad application prospects in the field of human vital sign monitoring. However, the challenge of achieving excellent performance while maintaining low cost and energy consumption remains a bottleneck in further expanding their application. This study endeavors to tackle the challenge by improving the performance of flexible poly(L-lactic acid) (PLLA) piezoelectric sensors and fabricating the encapsulation layer with a fish-scale microstructure (FSm) via the Digital Light Processing (DLP) technique. The FSm sensor presented herein exhibits a high sensitivity of 24.54 mV·kPa −1 and excellent linearity (R 2 > 0.99) in the low-pressure range (<20 kPa), with an optimal parameter design (200 µm distance and 400 µm height) of the microstructure. As a result, a 477% increase in sensitivity is obtained compared to conventional PLLA sensors. Simulation analyses are conducted to evaluate the impact of fish-scale orientation on the detection of human swallowing signals. The physiological pressure signals associated with the swallowing process are recorded using the FSm sensor in a wearable format. Moreover, the interference (additional) of vibration signals is effectively eliminated through further voice tests. The results indicated that FSm sensors hold promise for human swallowing health monitoring and action recognition, offering a novel approach to the treatment of swallowing disorders.
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FOOD CHEMISTRY
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2025
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IF 9.8
Jinpeng Zhu,Li Jiang,Jianhua Zhao,Yan Xiang,Yingyi Mao,Yunhao Lu,Yumeng Xia,Yuanping Lv,Qiang He
The combined cold plasma and sonication extraction (CPSE) was proposed for carotenoid extraction from Capsicum annuum L. wastes. CPSE was optimized based on response surface methodology, realizing the highest total carotenoids content of 45.24 ± 0.97 mg/100 g. UPLC-APCI-MS/MS confirmed 27 carotenoids in extracts, with lutein palmitate being the predominant compound. The carotenoid extracts (50 mg/mL) conveyed strong DPPH (77.45 % ± 0.61 %) and ABTS (89.60 % ± 1.69 %) radical scavenging capabilities. Compared with the control group, the acid and peroxide values decreased to 0.12 ± 0.03 mg/g and 0.022 ± 0.003 g/100 g in the extracts groups, respectively. The extraction mechanism involves cold plasma pretreatment disrupting the external structure of CPs, followed by sonication-assisted release of carotenoids, driven by hydrogen bonding interactions and conforming to the second-order kinetic model. Fried with carotenoid extracts improved visual and textural attributes of potato strips. This study is conducive to the value-added application of food waste.
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PubMed
高影响因子
Food Bioscience
·
2025
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IF 5.9
En Han,Ming Bao,Yang Li,Xiaoya Dong,Jianrong Cai
Developing simple and rapid methods for the detection of colorant content in foods is essential to mitigate their negative health effects on human. Hence, we constructed a biomimetic enzyme electrochemical sensor to efficiently detect sunset yellow using the nanocomposites of carbon black coated zinc oxide nanoflowers/poly (L-phenylalanine) (CB@ZnO/PLPA). CB was ultrasonically coated onto the surface of ZnO nanoflowers synthesized by two-step wet chemical method, and subsequently combined with PLPA for signal amplification. The synergistic effect of CB@ZnO/PLPA resulted in large electrochemical active area and excellent electrical conductivity. The investigations demonstrated a wide linear range of 0.5–200 μmol/L, a low detection limit of 0.07 μmol/L (S/N = 3) with a sensitivity of 9.272 μA μM −1 cm −2 , by the effective synergy between carbon black @ zinc oxide (CB@ZnO) and poly (L-phenylalanine) (PLPA). CB@ZnO/PLPA sensor showed satisfactory performance for SY detection and its feasibility was verified in real samples detection. In this paper, a simple and efficient electrochemical sensor for SY detection was developed, offering significant potential for widespread application in food analysis.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Mengmeng Wei,Leihua Jia,Dandan Fu,Zhenhua Shi,Masrina Mohd Nadzir,Junsheng Wang,Yipaerguli Yiminniyazi,Li Fan,Qiran Wang,Fude Liu,Bo Wang,Jinjun Yang
Poly(L-lactic acid) (PLA) is a biodegradable, biocompatible, and mechanically strong biomacromolecule with broad applicability across multiple areas. Nevertheless, its high flammability, tendency to melt-drip during burning, and inherently low crystallization rate limit its practical use. To address these issues, a halogen-free bio-based Schiff base flame retardant (VDPC) derived from vanillin and containing nitrogen and phosphorus was synthesized. With the incorporation of 10 % VDPC into the PLA, the composite achieved a limiting oxygen index (LOI) of 31.6 %, representing an increase of 56.4 %, and attained a V-0 rating in the UL-94 vertical burning test without dripping. Additionally, the peak heat release rate (pHRR), total heat release (THR), and carbon dioxide production (CO 2 P) reduced by 15.9 %, 36.8 %, and 29.7 %, respectively, confirming significantly enhanced fire safety. These improvements are attributed to synergistic flame-retardant mechanisms operating in both the condensed phase–through the formation of a relatively continuous and dense char layer–and the gas phase–via radical quenching by phosphorus–oxygen species. Furthermore, the composite showed accelerated crystallization rate, exceptional UV-blocking performance, and improved hydrophobicity. This study presents a sustainable and multifunctional flame-retardant system and demonstrates a viable approach to extending the utility of PLA-based materials in demanding applications.
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Food and Bioprocess Technology
·
2025
·Vol. 19
·期 1
·13
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IF 5.8
Bao Nina,Li Meng,Chen Ziyang,Zhou Wan,Rashed Marwan M. A.,Han Fangkai,Zhai Kefeng,Jiang Bianling
Pomegranate peel (PoP) is a valuable by-product rich in phenolic compounds with strong antioxidant potential. This study applied mechanochemical-assisted extraction (MCAE) to recover phenolics efficiently from PoP using eight solid reagents. The extraction parameters were optimized through response surface methodology based on a central composite design. Ultra-performance liquid chromatography coupled with triple time-of-flight mass spectrometry (UPLC-Triple-TOF/MS) tentatively identified twelve major phytochemicals. These phytochemicals include hydrolysable tannins, flavonoids, and phenolic acids. The optimal MCAE conditions were a liquid–solid ratio of 50 mL/g, an extraction temperature of 78 °C, and an extraction time of 67 min. Under these conditions, a total of 12 phytochemicals were identified and the total phenolic content reached 258.09 ± 1.35 mg GAE/g dry weight. The extract exhibited high antioxidant capacity (DPPH of 526.54 ± 13.38 μmol TE/g DW, ABTS of 1315.65 ± 15.16 μmol TE/g DW, FRAP of 798.57 ± 11.30 μmol TE/g DW, and RA of 1353.87 ± 23.43 μmol TE/g DW) and strong inhibitory activity against α-glucosidase and α-amylase. These results demonstrate that MCAE represents an eco-friendly and cost-effective technique for recovering bioactive phenolic compounds from PoP, offering promising applications as natural antioxidants and enzyme inhibitors in functional food formulations, providing new opportunities for the food industries. These findings provide a new strategy for valorizing agricultural by-products into functional ingredients for food applications.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
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IF 6.6
Kexin Wang,Wenqi Zhao,Hongxuan Zhao,Jing Xu,Sisi Gao,Shuang-kui Du,Xiaoli Gao
Selenium (Se) fortification is essential for meeting nutritional requirements and preventing Se deficiency-related diseases. This study evaluated the effects of sodium selenite (Na 2 SeO 3 ) and selenomethionine (SeMet) on mung bean sprouts (MBS) by investigating Se accumulation, distribution, and protein physicochemical properties. The findings indicated that the Se accumulation efficiency of SeMet treatment was 3.22 times that of Na 2 SeO 3 treatment. Furthermore, both treatments achieved an organic Se conversion rate exceeding 95%. Distribution analysis revealed that 72.39% to 78.50% of organic Se was incorporated into proteins, while 6.28% to 6.93% was bound to polysaccharides. The order of organic Se accumulation in protein components was albumin > glutelin > globulin > prolamin. Additionally, Se enrichment enhanced Se accumulation in low molecular weight protein (25-33 kDa) and promoted protein synthesis, but decreased the cysteine and methionine content. These changes led to an increase in β-turn, disulfide bonds, and surface hydrophobicity, while reducing the accessibility of sulfhydryl and pigment groups. Consequently, the oil absorption capacity and foaming capacity of the protein were enhanced. The results provide a foundation for the utilization of Se-enriched MBS protein as a functional food ingredient.
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COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING
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2025
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IF 8.9
Xiang Li,Wenjie Zhao,Guosong Wu,Yinghao Wu
The harsh service environment in the splash zone poses serious challenges for the long-term durability of steel pipe piles. The basalt fibers (BF) reinforced-composite coatings have acquired great attention in various fields such as offshore wind power equipment and construction industry due to the high strength and chemical stability. However, the service life of BF reinforced-composite coatings is greatly restricted by poor interfacial compatibility between BFs and polymer resin. In this work, we fabricated a BF reinforced epoxy powder composite coating with excellent erosion resistance by constructing L-Cys@Cu MOF on BFs. The interfacial shear strength value between MBF and EP was 49.66 % larger than that of BF. Contrasted to EP, the adhesion strength between 2.5 %MBF/EP coating and the steel substrate was 79.65 MPa, which was increased by 24.67 %. Interestingly, the mass and volume loss of 2.5 %MBF/EP coating was decreased by 22.77 % and 21.16 %, respectively, compared with 2.5 %CBF/EP coating. These results demonstrate the broader applicability of BF in composite materials, arising from the synergistic effect of mechanical interlocking between MBF and EP and the formation of cross-linking network between L-Cys@Cu MOF and EP. This work provides a novel perspective for the development of BF reinforced-composite coatings with long-term erosion resistance.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Yuting Wang,Ying Xiong,Hui Tian,Shi Wang,Qianying Zhang,Mingjing Zhang,Hong Ding,Ye Gu,Anlin Peng
A novel disposable sensor modified with MWNTs/Al 2 O 3 /poly-L-lysine film was developed for detection of clinical 17β-estradiol (17β-E 2 ) urine samples in this study. The electrochemical behavior of 17β-E 2 was greatly increased due to the good electrocatalytic activity of MWNTs/Al 2 O 3 /poly-L-lysine nanocomposites. The optimum experimental conditions such as scan rate, pH, accumulation time were obtained, and the peak potentials of 17β-E2 was explored at about 530mV. The peak current of 17β-E 2 measured by CV displayed the good linear property with the increasing of the concentrations. Under optimized conditions, the disposable sensor displayed excellent analytical performance with linear ranges of 17β-E 2 from 0.5nM to 50nM. And a very low detection limit of 0.014 nM was attained. Furthermore, the MWNTs/Al 2 O 3 /poly-L-lysine SPE was used to the rapid and successful determination of the hormone in spiked synthetic and real clinical pregnant woman urine samples with wonderful selectivity and sensitivity.
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