JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2026
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IF 5.3
Mengmeng Feng,Yongkang Luo,Yanfei Jiang,Chunyue Zhao
α-Ketoglutarate (AKG), ergothioneine (EGT), ascorbic acid (VC), and pyrroloquinoline quinone (PQQ) are increasingly co-formulated to combat age-related mitochondrial dysfunction and oxidative stress, driving an urgent need for reliable quality control. However, existing analytical approaches remain fragmented, targeting only one or two compounds, relying on time-consuming derivatization, or employing costly mass spectrometry, and critically failing to address chemical incompatibilities among analytes. This study presents the first validated HPLC-UV method for simultaneous quantification of all four bioactives. A segmented extraction strategy was developed to reconcile their physicochemical conflicts: VC, AKG, and EGT were extracted with 0.1% metaphosphoric acid, while acid-labile PQQ was separately extracted with neutral phosphate buffer. Chromatographic separation was achieved on an AQ-C18 column with a gradient of 50 mM Na₂HPO₄ and acetonitrile. The method demonstrated excellent linearity (R² > 0.99), high sensitivity, good precision (RSD < 2%), and satisfactory accuracy (recoveries: 97.2–103.6%). The Mosaic-WAC score of 82.6 further validates the proposed method. Analysis of commercial products indicated that only VC exhibited degradation, with more pronounced losses in liquid matrices, attributable to its inherent oxidizability. This underscores the need for comprehensive multi-component quality control. The proposed method provides a practical platform for routine analysis and regulatory evaluation of complex nutraceutical products.
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2026
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IF 2.5
Han Liu,Zhuo Li,Xilin Zhu,Ying Liu,XiaoPan Wu
Background & aims Caspase-10 (CASP10) participates in death receptor–mediated apoptosis, but the relevance of the CASP10 coding variant rs13006529 (T > A; p.Leu522Ile) to PEG-IFN-α response in chronic hepatitis B (CHB) is unknown. We assessed this association and whether differential apoptotic activity explains variant-associated HBV burden. Methods rs13006529 was genotyped in 262 patients receiving PEG-IFN-α with or without nucleos(t)ide analogues. Allele and genotype frequencies were compared by χ 2 tests; sustained virological response (SVR) was analyzed by univariable and multivariable logistic regression. HBV-infected HepG2-NTCP cells expressing empty vector or either CASP10 variant were compared; variant-expressing cells received vehicle or zVAD-fmk. Apoptosis was assessed by flow cytometry, caspase-3/7 activity, and Western blotting. HBV total RNA and pgRNA, intracellular DNA, cccDNA, and HBsAg/HBeAg were measured by RT-qPCR, qPCR, Southern blotting, and ELISA, respectively. Results The A allele was more frequent in patients without than with SVR (30.6% vs 17.1%; P = 0.002) and independently associated with lower odds of SVR (adjusted OR per A allele, 0.50; 95% CI, 0.27–0.86; P = 0.017). SVR rates were 36.9% in TT and 19.8% in AT/AA carriers (P = 0.003). Leu522-expressing cells showed greater apoptosis and lower HBV total RNA, pgRNA, intracellular DNA, and cccDNA than Ile522-expressing cells. zVAD-fmk attenuated the between-variant difference in apoptosis and partially narrowed the corresponding differences in HBV RNA and intracellular DNA. Conclusions rs13006529 was independently associated with PEG-IFN-α response. Differential caspase-dependent apoptotic activity partly accounts for variant-associated HBV burden differences.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2026
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IF 4.6
Yingying Chen,Zhihua Liu,Jinshan Liu,Miao Zhou,Zhifeng Liu
The visible-light-driven photoelectro-Fenton technology integrates the synergistic advantages of photoelectrocatalysis and the Fenton reaction. However, the design of current photoanode materials still suffers from issues such as low interfacial charge transfer efficiency and insufficient exposure of active sites. In this study, a novel S-scheme ZnIn 2 S 4 /α-Fe 2 O 3 heterojunction photoanode was successfully constructed. The built-in electric field established at the S-scheme heterointerface effectively facilitates the spatial separation of photogenerated charge carriers. The S O bonds formed at the two-phase interface serve as atomic-level electron transport channels, enabling rapid and directional transfer of photogenerated electrons enriched in the conduction band of ZnIn 2 S 4 to the Fe 3+ sites on the α-Fe 2 O 3 surface, where Fe 3+ is reduced to Fe 2+ . The generated Fe 2+ promotes the conversion of H 2 O 2 to produce hydroxyl radicals (·OH), while the regenerated Fe 3+ from the reaction is again reduced to Fe 2+ by photogenerated electrons, establishing a self-sustaining cycle that endows the system with highly efficient oxidative degradation capability. The results demonstrate that the synergistic mechanism leads to a 5.55-fold increase in the degradation rate. Benefiting from the efficient directional charge transfer and separation, the total organic carbon (TOC) removal efficiency reaches 80.09% within 60 min.
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JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
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2026
·Vol. 46
·期 10
·118263
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IF 6.3
Binbin Fan,Wei Sun,Dengke Zhao,Bohan Wang,Shuo Zhao,Feng Hu,Jie Zhang,Yiyao Ge,Zhipeng Xie,Kexin Chen,Guanghua Liu
Achieving a balance between high thermal conductivity and mechanical property remains a critical challenge for sintered reaction-bonded Si₃N₄ (SRBSN) ceramics. This work investigates the effects of α- and β-Si₃N₄ additions on nitridation behavior, microstructural evolution, and properties of SRBSN ceramics. The results reveal fundamentally different roles of α- and β-Si₃N₄ during nitridation and sintering. Moderate α-Si₃N₄ addition suppresses the α→β transformation, inducing localized grain growth and a bimodal microstructure at 20 wt%. In contrast, β-Si₃N₄ markedly promotes the α→β transformation and heterogeneous nucleation. The sample with 30 wt% β-Si₃N₄ exhibits a pronounced bimodal grain structure with 20.2 % coarse elongated grains (>6 μm), a maximum mean aspect ratio of 1.89, an ultrathin grain-boundary film (∼2.2 nm), and a nitrogen-rich intergranular phase. Consequently, a high thermal conductivity of 114 W·m⁻¹ ·K⁻¹ is achieved while maintaining a flexural strength of 791 ± 29 MPa and a fracture toughness of 9.0 ± 0.3 MPa·m 1/2 .
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Comparative Biochemistry and Physiology D-Genomics & Proteomics
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2026
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IF 2.9
Qianwen Liu,Jing Wang,Xiaofeng Jin,Hongyu Cheng,Guanyu Zhong,Song Wu,Yang Yang,Yanchun Sun
Carbonate alkalinity is a prevalent environmental stressor in inland waters and poses toxicological risks to aquatic organisms. While α-ketoglutarate (AKG) is known to alleviate the resulting intestinal injury in fish, its protective mechanisms remain unclear. Here, crucian carp ( Carassius auratus ) were exposed to carbonate-alkaline stress (20 and 40 mmol/L NaHCO 3 ) with or without exogenous AKG supplementation for 56 days. Histopathological examination, biochemical assays, 16S rRNA, and metabolomics were employed to investigate the effects of carbonate-alkaline stress on intestinal redox status, energy metabolism, and microbial community, and to evaluate the mitigating mechanism of exogenous AKG supplementation. Results indicated that carbonate-alkaline stress induced intestinal histopathological damage, with increased ROS and lipopolysaccharide, and decreased ATP levels. 16S rRNA sequencing revealed that carbonate-alkaline stress altered the gut microbial community, increasing Proteobacteria and decreasing Firmicutes abundance. Metabolomics indicated that energy pathways like glycolysis and TCA cycle were inhibited. Meanwhile, the activation of purine metabolism and inhibition of glutathione metabolism suggested intestinal redox imbalance. Notably, exogenous AKG supplementation alleviated these injuries. Metabolomics showed upregulation of key metabolites in glycolysis and TCA cycle, while abnormalities in purine and glutathione metabolism were mitigated. Additionally, the community structure shifted, decreasing Proteobacteria and increasing Firmicutes abundance. These findings indicate that carbonate-alkaline stress induces microbial dysbiosis and intestinal injury by disrupting energy and redox balance, a process mitigated by exogenous AKG supplementation which restores metabolic homeostasis. This study provides mechanistic insights into metabolic interventions alleviating intestinal toxicity under carbonate-alkaline stress and a theoretical basis for improving fish tolerance in carbonate-alkaline environments.
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ACS Sustainable Chemistry & Engineering
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2025
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IF 7.3
Jian Xu,Qidi Hu,Jia Cheng,Sheng Han,Jiusheng Li
Currently, commercial low viscosity polyalphaolefins (PAOs) are primarily produced by using traditional BF3-catalyzed systems. However, due to the catalyst’s toxic, corrosive, and environmentally hazardous gaseous properties, researchers are actively investigating alternative synthetic methods for low-viscosity PAO production. In this study, we present a novel approach that employs ethylaluminum sesquichloride-mediated hydro-alkylation to convert high-carbon α-olefin dimers (which are not suitable as direct lubricant base stocks) and chlorinated alkanes into ultralow viscosity and medium-high viscosity PAO products. Chlorinated hydrocarbons demonstrate dual reactivity in this process. By incorporating triethylsilane as an effective hydrogen donor and precisely controlling the olefin dimer/chloroalkane ratio, we successfully synthesized PAO2 and PAO3.5 with ultralow pour points (−78 °C) and a selectivity of 97%, achieving low-temperature performance that is comparable to commercial standards. Excess chlorinated hydrocarbons undergo β-hydrogen transfer, producing tertiary carbocations and α-olefins, which then undergo polymerization to yield PAO20 with an enhanced high viscosity index. Gas chromatography (GC) and nuclear magnetic resonance (NMR) analyses revealed that the steric hindrance of chlorinated hydrocarbons plays a significant role in influencing reaction activity and the architecture of the resulting products. Only terminal olefin adducts were detected. Molecular topology analysis indicated that compact molecular dimensions promote pour point depression in base oils. This study utilized hydro-alkylation to transform chlorinated alkanes into high-value PAO base oils, yielding ultralow viscosity polyalphaolefins with well-defined architectures. By achieving precise molecular control through substrate modulation, we established a novel technical approach for lubricant molecular engineering with a correlation between structure and performance.
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Journal of Materials Chemistry B
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2025
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IF 5.7
Yu Guan,Jinyu Liu,Jing Yang,Sufang Chen,Weifeng Yao,Shengyuan Gong,Chunsheng Xiao,Mingqiang Li,Gangjian Luo,Ziqing Hei
Hepatic ischemia-reperfusion injury (IRI) is characterized by an acute surge of reactive oxygen species (ROS) upon reperfusion, leading to oxidative damage and cell death. Ferroptosis, a form of iron-dependent lipid peroxidation-driven cell death, has recently been implicated in hepatic IRI, compounding the injury. Here, we present an oxidation-responsive nanoparticle system designed to mitigate liver IRI by scavenging ROS and inhibiting ferroptosis. We synthesized a PEGylated poly(α-lipoic acid) (PEG-PαLA) copolymer that self-assembles into nanoparticles encapsulating the lipophilic antioxidant coenzyme Q10 (CoQ10). The PEG-PαLA/CoQ10 nanoparticles have an average diameter of ~100 nm and release CoQ10 preferentially under oxidative conditions. In vitro, the nanoparticles efficiently neutralized free radicals and protected hepatocytes from oxidative injury. In a mouse model of partial hepatic IRI, treatment with PEG-PαLA/CoQ10 nanoparticles significantly reduced liver injury markers, preserved liver histology, and abrogated lipid peroxidation, indicating suppression of ferroptosis. Our results demonstrated that PEG-PαLA/CoQ10 nanoparticle is a novel antioxidant nanomedicine that synergistically attenuates ROS-mediated damage and ferroptotic cell death in hepatic IRI. These findings highlight a promising strategy for protecting organs from ischemia-reperfusion damage by targeting oxidative stress and ferroptosis with responsive biomaterial carriers.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Jing Gao,Hao Dong,Peichao Zhang,Kunpeng Gao,Haipeng Su,Jianan Sun,Xiangzhao Mao
Whole-enzyme cascade degradation of crystalline α-chitin is a long-term pursuit, which has been hampered by low transformation efficiency and heterogeneous products. Here, a novel β-N-acetylglucosaminidase (NAGase) from Sulfuriroseicoccus oceanibius, designated Sohex, was biochemically characterized. The enzyme demonstrated high hydrolytic activity toward N-acetyl chitooligomers (N-acetyl COSs) and exhibited promotive effects on crystalline α-chitin degradation. Furthermore, a whole-enzyme cascade degradation system based on lytic polysaccharide monooxygenases (LPMOs), chitinases, and Sohex was developed for N-acetyl-D-glucosamine (GlcNAc) production. This system achieved a maximum conversion rate of 45.93%, with GlcNAc purity reaching 99.99%. The addition of Sohex significantly enhanced α-chitin degradation efficiency, achieving conversion rates of 1.37-fold and 2.46-fold compared to the LPMOs + chitinases system and the sole chitinases system, respectively. Moreover, the promotion mechanisms of Sohex in conjunction with LPMOs and chitinases were elucidated, making the cascade system a promising strategy for converting crystalline α-chitin into high-purity GlcNAc.
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Materials Today Bio
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2026
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IF 11
Longqing Hu,Zhongyuan Yin,Songwei Tan,Yu Deng,Xincai Qu,Ting Hu,Qiong Wang
Triple-negative breast cancer (TNBC) derives only limited clinical benefit from radiotherapy (RT), partly due to abnormalities in the tumor microenvironment and dysregulated signaling pathways. By integrating TNBC cases from the METABRIC and TCGA cohorts, we implemented an analysis framework centered on RT-by-gene interaction terms, implicating TNF-α–NF-κB signaling and inflammatory response as potential effect modifiers of RT benefit. Based on a pathway-guided polyphenol–gene interaction screen, curcumin (Cur) was prioritized as a modulator of TNF-α–NF-κB/inflammation-related programs. We then engineered a multifunctional nanoplatform (TCC) that couples catalytic modulation of the tumor microenvironment with suppression of adaptive pro-survival programs. TCC was built on pH-responsive nanoceria (CeO 2 nanozymes) to modulate redox homeostasis and alleviate hypoxia, loaded with Cur, and coated with D-α-tocopherol polyethylene glycol succinate (TPGS) to improve stability and cellular uptake; cRGD was further introduced to generate cRGD-TCC for enhanced tumor targeting. In vitro and in vivo, TCC promoted G 2 /M-phase arrest, amplified RT-induced reactive oxygen species generation and DNA damage, and attenuated TNF-α–NF-κB/inflammatory programs and markers of inflammation, survival, and invasion. Collectively, TCC provides an integrated radiosensitization strategy that addresses microenvironmental barriers and adaptive tumor signaling, thereby potentially enhancing RT response in TNBC.
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CHEMISTRY & BIODIVERSITY
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2026
·Vol. 23
·期 5
·e71355
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IF 2.9
Zeyu Yi,Siyu Liu,Lincheng Bai,Fan Yang,Zhenkun Wang,Xiubo Zhu,Zunpeng Shu,Hua Han,Xiaojin Yu
Rosa davurica Pall. (RDP), a rosa fruit rich in bioactive compounds, has shown potential in alleviating cardiovascular diseases, yet its chemical composition and mechanisms against acute myocardial ischemia (AMI) remain unclear. This study comprehensively characterized 99 compounds in RDP extracts using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS), including flavonoids, terpenoids, and phenolic acids. By integrating transcriptomics and network pharmacology, we identified the tumor necrosis factor-alpha (TNF-α) signaling pathway as a key target in AMI progression. In vivo experiments demonstrated that RDP significantly ameliorated isoproterenol (ISO)-induced myocardial injury by reducing oxidative stress and suppressing the release of inflammatory cytokines (IL-6, IL-2, IL-17). Molecular docking and dynamics simulations further revealed that bioactive components in RDP, such as rutin and hyperoside, effectively bind to TNF-α and its receptor TNF receptor 1 (TNFR1), thereby modulating inflammatory responses. These findings provide valuable insights into the multi-target cardioprotective effects of RDP, supporting its application as a promising functional food. Graphical Rosa davurica Pall. (RDP) effectively ameliorates acute myocardial ischemia by neutralizing oxidative stress and disrupting the pro-inflammatory TNF-α/TNFR1 signaling axis. By integrating multi-omics profiling, molecular dynamics, and in vivo models, this study uncovers the multi-target cardioprotective mechanisms of RDP, establishing a robust scientific foundation for its application as a novel cardiovascular functional food.
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FOOD RESEARCH INTERNATIONAL
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2026
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IF 8.8
Zijun Li,Siyang Wang,Yanhui Wang,Liyan Shen,Shiyu Hu,Zhouguang Wang,Ruiqin Zhang,Zhen Li
DOP-W-1 was identified as a branched α-glucan from Dendrobium officinale . • DOP-W-1 alleviated DSS-induced colitis and preserved mucosal barrier integrity. • Gut microbiota depletion largely abolished the anti-colitic effect of DOP-W-1. • Fecal microbiota from DOP-W-1-treated mice transferred colitis protection. • DOP-W-1 restored SCFAs and corrected aromatic amino acid-related metabolism. A homogeneous polysaccharide, DOP-W-1, was isolated from Dendrobium officinale and identified as a low-molecular-weight branched α-glucan with a molecular weight of 9.76 kDa, mainly consisting of →4)-α-D-Glcp-(1 → and →4,6)-α-D-Glcp-(1 → residues. This study aimed to determine whether DOP-W-1 protects against dextran sulfate sodium (DSS)-induced colitis through gut microbiota-mediated mechanisms. In DSS-induced colitic mice, DOP-W-1 markedly alleviated disease severity, as shown by reduced body weight loss and disease activity index, restored colon length, improved histopathological injury, preserved mucus barrier integrity, decreased intestinal permeability, and rebalanced inflammatory cytokines. 16S rRNA sequencing revealed that DOP-W-1 selectively reshaped the gut microbiota, characterized by enrichment of Akkermansia and suppression of DSS-associated taxa, including Turicibacter and Alistipes . Importantly, antibiotic-mediated microbiota depletion largely abolished the protective effects of DOP-W-1, whereas fecal microbiota transplantation from DSS + DOP-W-1-treated donor mice reproduced the anti-colitic phenotype, demonstrating that gut microbiota is essential for its protective activity. Functional analysis further showed that DOP-W-1 restored microbial carbohydrate-active enzyme profiles associated with α-glucan utilization. Untargeted metabolomics and short-chain fatty acid analysis indicated that DOP-W-1 corrected aromatic amino acid-related metabolic disturbances, restored D-glucaro-1,4-lactone, and recovered short-chain fatty acid production. Collectively, these findings suggest that DOP-W-1 acts as a microbiota-targeted α-glucan that alleviates experimental colitis by restoring microbial carbohydrate metabolism, metabolic homeostasis, and mucosal barrier function. Download: Download high-res image (164KB) Download: Download full-size image
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Xiang Wang,Yejun Deng,Yang Zhang,Caihong Zhang,Lujie Liu,Yong Liu,Jianxin Jiang,Pujun Xie,Lixin Huang
Food-derived α-glucosidase inhibitory peptides have gained significant interest in treating type 2 diabetes mellitus (T2DM) owing to their favorable safety profiles. Molecular docking combined with molecular dynamics simulation was performed to screen α-glucosidase inhibitory peptides from Ginkgo biloba seed cake (GBSC), and two novel peptides (Met-Pro-Gly-Pro-Pro (MPGPP) and Phe-Ala-Pro-Ser-Trp (FAPSW)) were acquired. The results of molecular docking and molecular dynamics simulation suggested that FAPSW and MPGPP could generate stable complexes with 3wy1, and the electrostatic and van der Waals forces played contributory roles in FAPSW and MPGPP binding to 3wy1. The α-glucosidase inhibition assay corroborated that FAPSW and MPGPP had good α-glucosidase inhibition capacity, with IC50 values of 445.34 ± 49.48 and 1025.68 ± 140.78 μM, respectively. In vitro simulated digestion results demonstrated that FAPSW and MPGPP strongly resisted digestion. These findings lay a theoretical foundation for FAPSW and MPGPP in treating T2DM.
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危险品化学品经营许可证（带存储）