FASEB JOURNAL
·
2023
·Vol. 37
·期 5
·e22924
·
IF 4.8
Liangjie Lu,Huihan Wang,Minjie Yang,Lijun Wang,Kaifeng Gan
Beta-tricalcium phosphate (β-TCP) is considered as one of the most promising biomaterials for bone reconstruction. This study generated a functional molybdenum disulfide (MoS 2 )/polydopamine (PDA)/−bone morphogenetic protein 2 (BMP2)-insulin-like growth factor-1 (IGF-1) coating on the β-TCP scaffold and analyzed the outcomes. The MoS 2 /PDA-BMP2-IGF-1@β-TCP (MPBI@β-TCP) scaffold was prepared by 3D printing and physical adsorption, followed by characterization to validate its successful construction. The in vitro osteogenic effect of the MPBI@β-TCP scaffold was evaluated. It was found that MPBI@β-TCP augmented the adhesion, diffusion and proliferation of mesenchymal stem cells (MSCs). The alkaline phosphatase (ALP) activity, collagen secretion and extracellular matrix (ECM) mineralization along with the expression of Runx2, ALP and OCN were also enhanced in the presence of MPBI@β-TCP. Additionally, MPBI@β-TCP stimulated endothelial cells to secrete VEGF and promoted capillary-like tubule formation. We then confirmed the biocompatibility of MPBI@β-TCP to macrophages and its anti-inflammatory effects. Furthermore, under near-infrared (NIR) laser irradiation, MPBI@β-TCP produced photothermal effect to not only kill MG-63 osteosarcoma cells, but also enhance bone regeneration in vivo with biosafety. Overall, this work demonstrates that 3D-printed MPBI@β-TCP with enhanced osteogenic activity under NIR laser irradiation has a vast potential in the field of tissue defects.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Yaru Hu,Dandan Lei,Da Wu,Jing Xia,Wang Zhou,Changzheng Cui
The discharge of pharmaceutical wastewater introduces numerous pollutants into the environment, and their pollution level reduction has aroused extensive concern. This study investigated the variation in residual antibiotics and ecotoxicity to two nutritional-level model organisms in the pharmaceutical wastewater treatment process (PWTP). The wastewater in the equalization tank contained massive organic matters (2.9–18.7 times higher than the permissible values in GB21903-2008) and antibiotics (310.88 μg/L), posing extremely toxic effects to Vibrio fischeri ( V. fischeri ) and Daphnia magna ( D. magna ) . The biological anaerobic/aerobic treatment units contributed the most to the reduction of antibiotics and the ecotoxicity to both organisms, with the removal rates of 72% and > 90%, respectively. The ecotoxicity of pharmaceutical wastewater was strongly and positively correlated with the residual antibiotics, amoxicillin, cephalexin, ammonia nitrogen, and total phosphorus ( P < 0.05). However, the detected amounts of amoxicillin and cephalexin were approximately 10 5 times lower than the predicted no-effect concentrations of amoxicillin and cephalexin to V. fischeri and D. magna in freshwater, which implied the joint ecotoxicity posed by multicomponent mixtures, such as the residual antibiotics and organic toxic substances, rather than the specific residual antibiotics. This study provides a better understanding of the variations and residual levels of pollutants in PWTPs , including their ecotoxicity risk to the aquatic environment, highlighting the need to optimize pharmaceutical wastewater treatment technologies .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Xiang-Hua Ling,Yue-Feng Tang,Guo-Zhi Han
The composite of hydrogels and noble metal nanoparticles has wide applications in the field of functional materials . In this paper, we developed a novel and green strategy to synthesize a kind of composite hydrogel microspheres containing silver nanoparticles and β-cyclodextrin (β-CD) modified sodium alginate (β-CD@SA@Ag) without use of strong reducing reagent. First, β-CD was chemical linked to SA by epichlorohydrin (ECH) to form the β-CD modified polymer (β-CD@SA). then AgNO 3 was added to the as-prepared polymer solution , in which silver nanoparticles were formed by the reducing power of β-CD units. Subsequently, by reversed-phase emulsion method and ionic cross-linking, a kind of β-CD@SA@Ag hydrogel microspheres were obtained. The structure and morphology of the composite material were characterized in detail by Fourier infrared spectroscopy (FT-IR), thermogravimetric analysis (TG), scanning electron microscope (SEM), transmission electron microscope (TEM) and X-ray diffraction (XRD). Test data indicated that silver nanoparticles evenly dispersed in the composite material. On the basis, the adsorption and catalytic performances of the β-CD@SA@Ag hydrogel microspheres were studied towards to the reduction of aromatic nitro compounds. Research results showed that the β-CD@SA@Ag hydrogel microspheres had excellent catalytic activity along with high environmental adaptability. whether in pure water, tap water or river water, aromatic nitro compounds can be completely reduced within 2–13 min. Moreover, the morphology of microspheres of the composite material endows the composite material with high stability and reusability. We believe that our work would provide a new idea for the green synthesis of organic-inorganic composite functional materials .
✓ 已验证
产品关联
DOI
高影响因子
CRYSTENGCOMM
·
2018
·Vol. 21
·期 3
·513-523
·
IF 3.304
Xiao Liu,Naru Zhao,Xiaoheng Guo,Haibo Duan,Jingjing Diao,Yifan Dong,Yingjun Wang
Cellular behavior is a result of homologous responses to biomaterials, especially material surfaces. An effective way to elicit a desired biological response is to employ micro/nanostructured constructions for biomaterial surface design; however, the brittleness of inorganic materials usually hinders structure regulation. In this study, a micro/nanostructured surface was fabricated using a hydrothermal treatment that did not require crystal regulation reagents. The precursor, β-tricalcium phosphate, was mixed with CaSiO3. The resulting micro/nanostructured surface had a hybrid structure containing nanowires, microrods, and rod clusters. In vitro cellular proliferation, cytotoxicity and osteodifferentiation experiments indicated that the micro/nanostructured surfaces alleviated material cytotoxicity, improved cell proliferation, and effectively promoted osteogenic differentiation of mouse bone mesenchymal stem cells. These results suggest that this simplified surface treatment is an efficient way to improve cell biological response to inorganic biomaterials.
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Chennan Zheng,Jiahao Li,Yijin Chang,Dazhi Zhou,Changchun Hao
Ligand glycosylation is often assumed to enhance protein binding by increasing hydrogen-bonding capacity, but its actual role in coumarin–β-lactoglobulin (β-LG) recognition has not been clearly resolved. Here, fraxin (FXN) and its aglycone fraxetin (FXT) were used as a paired model to examine how a glucose moiety regulates β-LG binding through multispectroscopic analysis and molecular simulations. Both ligands associated with β-LG and quenched its intrinsic fluorescence, but FXT showed stronger quenching, higher apparent affinity, a shorter FRET-derived donor–acceptor distance, and a more pronounced reduction in fluorescence lifetime. Thermodynamic analysis indicated spontaneous, entropy-favored binding for both systems. Synchronous and three-dimensional fluorescence spectra further showed that FXT caused greater perturbation of the aromatic-residue microenvironment, whereas far-UV CD revealed only limited secondary-structure fluctuations without a consistent concentration-dependent trend. Docking, MD simulation, and MM/PBSA calculations indicated that the glucose group in FXN introduced additional polar contacts but also a larger polar-solvation penalty and possible steric constraints. In contrast, FXT achieved closer hydrophobic accommodation near the fluorescence-sensitive region of β-LG. These results suggest that glycosylation does not simply strengthen coumarin–β-LG binding, but reshapes molecular recognition through a trade-off among polar interactions, desolvation, and hydrophobic fitting.
✓ 已验证
DOI
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2023
·
IF 5.6
Runxiao Lv,Lili Du,Lunhao Bai
Osteoarthritis (OA) is a chronic joint disease characterized by the progressive degradation of articular cartilage. In this study, as determined by histological staining, the cartilage surface of the OA rats was damaged, defective and broken, and chondrocytes and proteoglycan were reduced. While moderate physical exercise showed protective effects on the cartilage. Besides, RNA-seq was performed to select a target protein and RNF125 (an E3 ubiquitin ligase) was decreased in the cartilage tissues of OA rats and increased after physiological exercise. However, the precise role of RNF125 in OA is still unknown. This work aimed to investigate the involvement and underlying mechanism of RNF125 in OA pathogenesis. Our results defined that adenovirus-mediated overexpression of RNF125 inhibited the degradation of extracellular matrix of chondrocytes induced by IL-1β, as revealed by increased chondrocyte viability, upregulated COL2A1 and ACAN levels, and downregulated MMP1, MMP13 and ADAMTS5 levels, which was abrogated by NR4A2 knockdown. In vivo, RNF125 relieved OA, manifested as reduced cartilage injury and increased chondrocytes. Mechanically, NFATC2 bound to the RNF125 promoter and directly regulated RNF125 transcription, as illustrated by luciferase reporter, Ch-IP and DNA pull-down assays. Furthermore, RNF125 overexpression inhibited the nuclear translocation of β-catenin, thus suppressing activation of the Wnt/β-catenin signaling pathway. Also, RNF125 as E3 ubiquitin ligase led to the ubiquitination and degradation of TRIM14 protein, and TRIM14 overexpression efficiently reversed the effects of RNF125 overexpression on OA progression. Totally, this study provides new insights into OA pathogenesis regulated by RNF125. RNF125 may be a novel biomarker for OA therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS RESEARCH BULLETIN
·
2023
·
IF 5.4
Song Wu,Junli Wang,Xuanbing Wang,Di Jiang,Jinlong Wei,Xiaoning Tong,Zhenwei Liu,Qingxiang Kong,Naixuan Zong,Ruidong Xu,Linjing Yang
s Ti-based PbO 2 electrodes must currently address the high overpotential and low corrosiveness of the anodic oxygen evolution reaction (OER) during zinc electrowinning. In this study, a composite electrode composed of Ti/TiH 2 /β-PbO 2 − Mn 3 O 4 @C (0.6 g L –1 ) was fabricated using Ti/TiH 2 generated by electrochemical reduction of Ti/TiO 2 prepared by laser ablation as an intermediate layer, co-deposited β-PbO 2 , and Mn 3 O 4 @C micro-flakes prepared by high-temperature pyrolysis-oxidation as the active layer. In a zinc electrowinning test solution (50 g L –1 ZnSO 4 mixed with 150 g L –1 H 2 SO 4 ), the oxygen evolution and corrosion behavior of the obtained Ti/TiH 2 /β-PbO 2 − Mn 3 O 4 @C composite anodes were systematically investigated. Ti/TiH 2 /β-PbO 2 − Mn 3 O 4 @C (0.6 g L –1 ) exhibited a low overpotential of 493 mV, a low Tafel slope of 73.6 mV dec −1 , a low i corr of 2.320 × 10 −5 A cm −2 and voltage stability of 1.65 V vs. RHE for up to 72 h in stability tests, with an overall performance outperformed most of the reported Ti-based PbO 2 electrode materials. The outstanding catalytic activity is primarily attributable to the low resistance and porous interlayer of TiH 2 nanosheets, which speeds up the rate of electron transfer. The addition of Mn 3 O 4 @C micro-flakes to the active layer enhances the composite electrode's OER performance and corrosion resistance.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2019
·
IF 6.054
Lan-Lan Lou,Fu-Qiang Ni,Lin Chen,Sharpkate Shaker,Wei Li,Rong Wang,Gui-Hua Tang,Sheng Yin
A germacrane sesquiterpenoid library containing 30 compounds (2–31) was constructed by structural modification of a major component aristolactone (1) from the traditional Chinese medicine Aristolochia yunnanensis. Compound 11 was identified as a promising anticardiac fibrosis agent by systematic screening of this library. 11 could inhibit the expression of fibronectin (FN), α-smooth muscle actin (α-SMA), and collagens in transforming growth factor β 1 (TGFβ1)-stimulated cardiac fibroblasts at a micromolar level and ameliorate myocardial fibrosis and heart function in abdominal aortic constriction (AAC) rats at 5 mg/kg dose. Mechanistic study revealed that 11 inhibited the TGFβ/small mother against decapentaplegic (Smad) signaling pathway by targeting TGFβ type I receptor (IC50 = 14.9 ± 1.6 nM). The structure–activity relationships (SARs) study indicated that the unsaturated γ-lactone ring and oxidation of C-1 were important to the activity. These findings may provide a new type of structural motif for future anticardiac fibrosis drug development.
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Wenwen Bie,Shuzhao Zhang,Lin Zhang,Hengye Li,Xiaoyu Sun,Tianpei Cai,Zhongxia Wang,Fenying Kong,Wei Wang
Herein, a thioether-functionalized porous β-cyclodextrin polymer (P(Bn-S-CD)) was prepared for efficient removal of heavy metal ions and organic micropollutants (OMPs) from water. P(Bn-S-CD) showed a surface area of 763 m 2 /g and a sulfur content 5.83 wt%. Based on screening studies, Hg 2+ and diclofenac sodium (DS) were selected as model pollutants. P(Bn-S-CD) could adsorb Hg 2+ and DS simultaneously, while the adsorbed Hg 2+ afforded positive charges to the primary rims of CDs, greatly enhancing the adsorption rate and adsorption capacity of DS. Although the adsorbed DS showed no obvious effect on Hg 2+ adsorption, it improved the affinity of Hg 2+ upon P(Bn-S-CD). Adsorption mechanism studies confirmed the essential role of electrostatic interactions for these results. P(Bn-S-CD) also showed good selectivity towards heavy metal ions, excellent adsorption performance in real water at environmental levels and good reusability, implying great promise for water treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2023
·
IF 9.8
Shihao Su,Guojun Lv,Xuyang Zou,Jiangzhang Wang,Chaoyi Zhou,Yan Chen,Jialing Shen,Yangbin Shen,Zhongmin Liu
In the cyclic alkene epoxidation reactions using molecular oxygen as oxidant, aldehyde is generally added as co-oxidant to assist transition metal catalyst to activate the oxygen molecule, oxidizing cyclic alkene and producing the corresponding epoxide; however, the added aldehyde would be converted into byproduct carboxylic acid. Herein, we report the room-temperature tandem conversion of cyclic alkenes into 1,2-diols through the application of byproduct carboxylic acid as a catalyst for epoxide hydration reaction, realizing effective utilization of the byproduct and saving energy consumption. Four kinds of nanosized MnO2 specimens, including α-, β-, δ-, and γ-MnO2, were hydrothermally synthesized and applied as heterogeneous catalysts for the room-temperature tandem conversion of cyclohexene into 1,2-cyclohexanediol using molecular oxygen as oxidant and isobutyraldehyde as co-oxidant. The characterization results demonstrated that the obtained β-MnO2 sample had the maximum Mn4+/Mn3+ ratio of 1.42 and the minimum Oads/OLatt ratio of 0.34, consistent with its least oxygen vacancies evidenced by EPR measurement. Accordingly, the β-MnO2 sample revealed the quickest alkene epoxidation reaction rate and could achieve a 99.2% cyclohexene conversion within 1 h, and meanwhile, a 73.6% 1,2-cycloheanediol yield could be attained within 24 h. A plausible mechanism for the tandem conversion of cyclohexene into 1,2-cyclohexanediol over the β-MnO2 heterogeneous catalyst was proposed and further verified by quenching experiments and DFT calculations. Furthermore, the synthesized β-MnO2 catalyst sample could be reused more than ten times, and meanwhile, the tandem conversion way could be expanded to most of the cyclic alkene substrates, which demonstrated that the β-MnO2 catalyst was an active and stable heterogeneous catalyst for tandem conversion of cyclic alkenes into 1,2-diols.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·
IF 4.1
Jing Cheng,Guangwen Zhang,Liu Liu,Jianming Luo,Xichun Peng
BACKGROUND Beta-glucan is widely sourced and has various physiological activities including anti-inflammation. However, the strength of the anti-inflammatory activity of β-glucans from different sources remained elusive due to the lack of rapid and effective biomarkers. Therefore, this study aimed to screen out the β-glucan with strong anti-inflammatory activity from 5 different sources and to further screen out possible biomarkers in metabolites after fermented the β-glucans by gut microorganisms. RESULTS The results showed that all five β-glucans inhibited the production of LPS-induced pro-inflammatory mediators and suppressed the mRNA expression level of TLR4/MyD88. Their anti-inflammatory mechanisms involved the inhibition of intracellular ROS production and suppression of mRNA expression of the NF-κB pathway and JNK pathway. Among them, barley β-glucan exhibited the strongest anti-inflammatory effect, followed by Ganoderma lucidum β-glucan. Enhanced anti-inflammatory activity of β-glucan after fermentation was found and may be related to the increased abundance of metabolites such as the vanillin, dihydroxyphenylacetic acid, caffeic acid, acetic acid, butyric acid, and lactic acid. They are strongly positively correlated to the abundance of beneficial bacteria such as Blautia , suggesting that the production of those metabolites may be responsible for the flourishing of the beneficial bacteria. CONCLUSION In conclusion, barley was a preferred raw material for the preparation of β-glucans with high anti-inflammatory activity. Vanillin, dihydroxyphenylacetic acid, caffeic acid, acetic acid, butyric acid, and lactic acid were the possible biomarkers that could be utilized to evaluate the anti-inflammatory effect of β-glucans. This article is protected by copyright. All rights reserved.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Yingying Zhang,Carice Chong,Wangshu Tong,Haitao Li,Hiang Kwee Lee,Jie Han
Polyvinylidene fluoride (PVDF) is promising for piezo-catalytic applications owing to its excellent biocompatibility, flexibility, and durability. However, it is limited by weak electroactivity originating from its intrinsically low β piezoelectric phase content (<20%) and insulating properties. Here, we achieve efficient piezo-catalysis by introducing a self-polarized PVDF-based composite containing carbon nanotube (CNT) as conductive fillers. Notably, our self-polarized CNT/PVDF (SCP) ensemble exhibits a high β phase content of ∼95%, which is up to 6-fold higher than the original PVDF powder, and superior electrical activity by exploiting the polarity induction effect of water molecules. As a proof-of-concept demonstration, the SCP realizes efficient degradation of organic-polluted water by at least 95% within 2 h of ultrasonication (240 W), even at high pollutant concentration (40 mgL −1 ). Systematic investigations unveil its excellent recyclability and adaptability to various tropical or subtropical natural waters. More importantly, the SCP also achieves efficient H 2 O 2 production of up to ∼13.5 mmol g CNT −1 h −1 , which is > 6-fold and > 19-fold better than standalone self-polarized PVDF (i.e., without CNT content) and emerging piezo-catalytic designs, respectively. This study offers a unique approach for designing PVDF-based piezo-catalyst to expedite mechanically-driven catalysis towards practical applications.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）