MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Nuo Chen,Zhiyuan Xing,Hongying Liu,Lu Fang,Yuqing Zhang,Lihua Li,Zheng-Zhi Yin,Maofeng Wang
Reduced nicotinamide adenine dinucleotide (NADH) plays crucial roles in maintaining cell growth and differentiation, cytoprotection and energy metabolism. And it is imperative to precisely monitor the variable quantity of NADH. Herein, the connotative feature of acupuncture needle (AN) was explored as electrochemical matrix. Poly-3, 4-ethylenedioxythiophene-liganded gold nanoparticle modified AN electrode (ANE/AuNPs/PEDOT) was prepared via a simple electrosynthesis technology. Working together by charge transfer and reaction has a strong response to the electron-transfer of “N” in NADH, which is coordinately presented as a current peak. Interestingly, the behavior of electron-transfer was affected by ambient environment. After the conditional optimization, the fabricated sensor exhibited good linear range of 20 ∼ 500 μmol/L with a detection limit of 18.5 μmol/L. In addition, the sensor can be successfully applied to analysis NADH in serum. Furthermore, the microelectrode exhibits the real-time monitor ability for NADH that released from MDA-MB231 cells. This sensor also possesses the significant performance of high selectivity, stability and reproducibility. Based on the acupuncture history, the developed ANE should be a fascinating microsensor besides the low price, fast response and user-friendly operation.
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高影响因子
Food & Function
·
2024
·
IF 5.1
Lianghua Xie,Xin Hao,Jiahong Xie,Jianling Mo,Changzheng Yuan,Wei Chen
Nonalcoholic fatty liver disease (NAFLD) is a worldwide public health issue, but a widely accepted therapy is still lacking until now. Anthocyanins are natural flavonoid compounds that possess various bioactivities, but their applications are limited due to their low bioavailability and stability. Acylated anthocyanins are reported to show higher stability, whereas their effects on NAFLD are still unclear. Herein, pelargonidin-3-O-(6′′-acetyl)-glucoside (Ace Pg3G) was found to dose-dependently reduce intracellular lipid droplets and triglycerides, and improve cellular oxidative stress that accompanied lipid deposition. Besides, Ace Pg3G was proved to activate AMPK phosphorylation, thus stimulating AMPK-mediated lysosome-autophagy pathway to eliminate overloaded lipid. Further study unveiled that Ace Pg3G regulated genes related to lipid metabolism downstream of AMPK to inhibit lipid synthesis and accelerate lipid oxidation. Overall, this study provided the first evidence, to our best knowledge, that Ace Pg3G ameliorated free fatty acid-induced lipid deposition in hepatocytes through regulating AMPK-mediated autophagy pathways and redox state.
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2012
·
IF 2.484
Da-Quan Sun,Ying Wang,Ding-Gan Liu
AU-rich elements are functional motifs in the 3′untranslated region of mRNA and are binding sites for the RNA binding protein HuR, an mRNA stabilizer and translation enhancer implicated in carcinogenesis. It is not clear whether, and, if so, how the AU-rich elements function in cells when they are separated from their mRNA and form an independent RNA species. Here, we show that a short RNA with AU-rich elements derived from C/EBPβ 3′UTR suppressed growth in a human liver cancer cell line. It specifically bound HuR, and it competed with C/EBPβ mRNA in order to bind to HuR. Our results provide evidence that the cancer cell growth suppression by this 62nt RNA containing AU-rich elements may be due to competitive binding to HuR. This work may open new options for the development of novel anti-cancer drugs.
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-17
·
IF 5.3
Liang Jintao,Liang Jianlu,Xiao Xinkai,Guo Fei,Zhan Yulian,Zhou Xueqing,Zhou Zhide,Li Guiyin
A sandwich-type electrochemical aptasensor for ultrasensitive detection of glypican-3 (GPC3) was constructed using GPC3 aptamer (GPC3 Apt ) labelled reduced graphene oxide-cerium oxide-gold nanoparticles (RGO-CeO 2 -Au NPs) as the signal probe and the same GPC3 Apt as the capture probe. The electrochemical redox properties of CeO 2 (Ce 3+ /Ce 4+ ) in the RGO-CeO 2 -Au NPs indicate the electrochemical signals. When the target GPC3 was present, an “aptamer-protein-aptamer” sandwich structure was formed on the sensing interface due to the specific binding between the protein and aptamers, resulting in an increased electrochemical redox signal detected by differential pulse voltammetry (DPV) technique. Under optimal conditions, the established aptasensor exhibited a logarithmic linear relationship between the current response and GPC3 concentration in the range 0.001–100.0 ng/mL, with a minimum detection limit of 0.74 pg/mL. Using the spike-recovery tests for measurement of the human serum samples, the recovery was from 99.26 to 114.01%, and the RSD range was 3.04 to 5.34%. Furthermore, the sandwich-type electrochemical aptasensor exhibited excellent performance characteristics such as good stability, high specificity, and high sensitivity, demonstrating effective detection of GPC3 in human serum samples and can be used as a clinical detection tool for GPC3. Graphical Abstract
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xiaohong Tan,Wenzhan Li,Wei He,Tingting Yu,Bingbing Wan,Yong Huang,Jintao Liang,Guiyin Li
Glypican-3 (GPC3), which is a surface heparan sulfate proteoglycan, has been widely known the ideal biomarker for diagnosis and treatment of hepatocellular carcinoma (HCC). Accurate and sensitive detection of serum GPC3 level is an important and challenging task. In this paper, a sandwich electrochemical aptasensor was designed to specifically detect serum GPC3 level through aptamer-target-aptamer recognition. A GPC3 aptamer (GPC3 Apt ) immobilized on the surface of gold nanoparticles@reduced graphene oxide modified screen-printed electrode (Au NPs@rGO/SPCE) was used as the capture probe. Hemin-reduced graphene oxide-carboxymethyl chitosan@platinum nanoparticles (H-rGO-CMC@Pt NPs), with good mimicking peroxidases activity, were labeled with amination GPC3 aptamer, which was employed as the signal probe. Once the target GPC3 was anchored on the surface of GPC3 Apt /Au NPs@rGO/SPCE, the H-rGO-CMC@Pt NPs-GPC3 Apt was further specifically bound to the GPC3, forming the aptamer-target-aptamer sandwich structure, which can catalyze the oxidation of hydroquinone (HQ) to benzoquinone (BQ) and lead the oxidation current of HQ recorded by DPV to changing. The linear relationship between the current signal of oxidation of HQ and GPC3 concentration range of 0.0001–3.0 µg/mL and 3.0–60.0 µg/mL, with a low detection limit (LOD) of 0.0685 ng/mL. Additionally, the electrochemical aptasensor was successfully applied for the detection of GPC3 in human serum samples with the recovery of 99.95–104.06 % and relative standard deviation (RSD) of 1.31–5.22 %. Thus, the sandwich aptasensor could provide a new strategy for detection of serum GPC3 level and improve the early diagnosis rate of HCC.
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Materials Today Chemistry
·
2025
·
IF 6.7
Yipin Zhang,Guitao Tan,Haonan Hu,Qinqin Zhang,Jing Tu
Bacterial infection and excessive production of free radicals, including reactive oxygen species (ROS), reactive nitrogen species (RNS), are the main factors for impaired wound healing. Development of a nanomedicine with antibacterial, antioxidant, and anti-inflammatory activities is a promising treatment modality for infected wounds. Herein, we fabricated a versatile nanomedicine with antibacterial and free radical eliminating properties through a facile layer-by-layer strategy. Polydopamine nanoparticles (PDA NPs) were first prepared as core materials, followed by in situ formation of Prussian blue (PB) and coating with epigallocatechin-3-gallate (EGCG)-derived polymer layer successively, resulting in a core-shell structured nanomedicine (designated as PDA@PB@EGCG). The integration of PDA core and uniformly decorated PB endowed as-prepared PDA@PB@EGCG with excellent NIR-triggered photothermal properties. In addition, owing to the multi-enzymatic activity of PB and potent antioxidant capacity of PDA and EGCG, PDA@PB@EGCG with robust ROS/RNS-eliminating properties exhibited a strong cytoprotective effect against oxidative damage. Under near-infrared (NIR) laser irradiation (808 nm, 1.5 W/cm 2 ), PDA@PB@EGCG (50 μg/mL) displayed a robust and broad-spectrum bactericidal activity against Escherichia coli ( E . coli ) and Staphylococcus aureus ( S . aureus ). In vivo therapeutic efficacy was evaluated using a S . aureus -infected rat model, indicating PDA@PB@EGCG could effectively eliminate bacteria, modulate the infectious microenvironment and promote wound healing.
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ENERGY & FUELS
·
2017
·
IF 3.091
Cheng Sun,Shujing Wen,Jingkai Zhao,Chongjian Zhao,Wei Li,Sujing Li,Dongxiao Zhang
In this work, the blends of 1-hydroxyethyl-3-methylimidazolium glycine ([C2OHmim][Gly]) synthesized by our laboratory and N-methyldiethanolamine (MDEA) in aqueous solution were prepared for CO2 capture, and the maximum absorption performance of the blends was obtained at the mole ratio of 8:2 of MDEA/[C2OHmim][Gly] with a total concentration of 1.0 mol L–1. CO2 loading of the blended absorbent was less adversely influenced by the temperature and O2 concentration than that of MDEA aqueous, and it had a good performance in regeneration ability. The reaction mechanism of the CO2 absorption in MDEA/[C2OHmim][Gly] was investigated by 13C nuclear magnetic resonance. CO2 first reacted with [C2OHmim][Gly] to form carbamate, and then carbamate promoted the reaction between CO2 and MDEA, which could be described as a shuttle mechanism. The kinetics of CO2 absorption was investigated in a double stirred-cell absorber at different temperatures, and some important kinetic parameters were obtained, such as the reaction rate constant (k2) and the overall rate constant (kov). Experimental results indicated that the addition of [C2OHmim][Gly] enhanced CO2 absorption of MDEA under low CO2 partial pressure, which could improve the application of MDEA in industry.
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ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE
·
2025
·Vol. 651
·期 3
·e202400210
·
IF 1.1
Ziyue Zhang,Zhuoer Cai,Yinan Zhang,Jian Chen,Xiu-Ni Hua,Baiwang Sun
The focus of this study is on the development of novel host–guest inclusion compounds, which is a crucial molecule model for the synthesis of dielectric phase transition materials. In this research, a new host–guest inclusion compound is successfully synthesized by selecting 18-crown-6 as the host and 3-aminocyclobutanone as the guest molecule. The thermal behavior and crystal structure of the compound are analyzed in this article. It is found that the compound exhibits a reversible phase transition near 147 K, accompanied by a dielectric anomaly, exhibiting dielectric switching characteristics. The 18-crown-6 molecule and the 3-aminocyclobutanone molecule are connected via hydrogen bonding. Furthermore, the enthalpy changes of the heating process and cooling process are also calculated. This finding is expected to yield valuable new insights into the design of host–guest inclusions.
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CRYSTENGCOMM
·
2024
·Vol. 26
·期 26
·3501-3508
·
IF 2.6
Manman Wang,Xing Zhang,Zifeng Wang,Dongze Li,Shusen Chen,Qinghai Shu
3-Nitro-1,2,4-triazole-5-one (NTO) has received wide attention and has applications owing to its excellent detonation performance and low sensitivity. A new crystal form of NTO was prepared via the solvent evaporation method and named γ-NTO. Its crystal properties were studied through single-crystal X-ray diffraction, and the results indicated that the unit cell of γ-NTO belongs to the monoclinic space group Pc, with eight molecules in the single unit cell. The density of this crystal is calculated to be 1.907 g cm−3, which is higher than that of β-NTO and similar to that of α-NTO. The crystal morphology was calculated to be a polygonal shape with a symmetrical growth pattern using the reactive force field. Based on the analysis of reactive molecular dynamics, the thermal decomposition mechanism of different crystal forms of NTO was elucidated, and the results showed that γ-NTO demonstrates higher gas production, providing a successful way to enhance detonation performance. The results of IR and differential thermal analysis (DTA) support the above conclusions.
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JOURNAL OF SOLID STATE CHEMISTRY
·
2024
·
IF 3.2
Le Chen,An Xie,Nan Zhang,Jianjun Yin,Qun Chen,Mingyang He,Zhihui Zhang
Zeolitic-Imidazolate Framework 8 (ZIF-8), a porous crystalline absorbent, could be synthesized from the coordination reaction of zinc ions and 2-methylimidazole (2-mIM). ZIF-8 has a large specific surface area and a high adsorption capacity for n -hexane ( n HEX). However, ZIF-8 suffers from low selectivity for both linear and mono-chain alkanes. Therefore, ZIF-8 was modified through 2-mIM ligand being partly replaced by 2-nitroimidazole (2-nIM) through Vapor-Phase post-synthesis modification. The modified materials were denoted as ZIF-8-2-nIM-X (X = 24h, 48h, 72h). ZIF-8-2-nIM-X has a higher specific surface area and better thermal stability than ZIF-8. Further thermodynamic and kinetic calculations show that the affinity of hexane isomers on ZIF-8-2-nIM-X is spontaneous physical adsorption combined with an exothermic process. In contrast, kinetic studies show that the adsorption of hexane isomers on ZIF-8-2-nIM-X is a pseudo-second-order kinetic model. The batch and dynamic adsorption and separation properties of ZIF-8-2-nIM-X for hexane isomers were studied. The results of the one-component vapor adsorption equilibrium experiment show that ZIF-8-2-nIM-72h has the best adsorption separation coefficient at 293 K. The adsorption capacity of n HEX and 3-methylpentane (3MP) were 211.63 mg/g and 33.91 mg/g, respectively. Compared with ZIF-8 (selectivity coefficient: 1.08), the adsorption selectivity coefficient of the modified material is greatly improved. The adsorption selectivity coefficient of n HEX/3MP is 6.24 (ZIF-8-2-nIM-72h).
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POLYHEDRON
·
2024
·
IF 2.4
Zhengwei Wu,Jiawen Bao,Hongyang Zhang,Yingying Luo,Zhonglu You
Molecular nanoclusters comprising heterometallic Ni-Zn cores are an interesting class of compounds. In this paper, five novel Ni II -Zn II complexes [Ni 2 Zn 2 ClL 3 (HL) 3 ]·0·.25H 2 O ( 1 ), [Ni 2 Zn 2 BrL 3 (HL) 3 ]·0·.25H 2 O ( 2 ), [Ni 2 Zn 2 Br 2 L 2 (N 3 ) 2 (MeOH) 2 ]·2MeOH ( 3 ), [Ni 2 Zn 2 I 2 L 2 (N 3 ) 2 (MeOH) 2 ]·2MeOH ( 4 ), and [Ni 2 Zn 2 I 2 L 2 (N 3 ) 2 (DMF) 2 ] ( 5 ), were prepared from the Schiff base 2-(3-ethoxy-2-hydroxybenzylideneamino)-4-methylphenol (H 2 L). The complexes have been characterized by elemental analysis, IR, UV–Vis spectra. Molecular structures of the complexes have been determined by single crystal X-ray determination. In complexes 1 and 2 , three of the Schiff bases coordinate to the metal atoms via ether oxygen, imino nitrogen and two phenolate oxygen atoms, while the fourth one coordinates through ether oxygen, imino nitrogen and one phenolate oxygen atoms. In complexes 3 , 4 and 5 , all the donor atoms in the Schiff base ligands participate in coordination. The Ni and Zn atoms in the complexes are in octahedral and trigonal bipyramidal coordination, respectively. The anions of the metal salts and the azide anion, as well as methanol and DMF molecules furnish the coordination sphere. The compounds were evaluated for their antibacterial activities on Bacillus subtilis , Staphylococcus aureus , Escherichia coli and Pseudomonas fluorescens , and antifungal activities on Candida albicans and Aspergillus niger . Interestingly, the complexes have effective activities against Bacillus subtilis and Staphylococcus aureus .
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Food Science & Nutrition
·
2025
·Vol. 13
·期 8
·e70756
·
IF 3.8
Jie Shi,Jingyi Ge,Zhenghai Zhang,Lianhui Wei,Yan Dong,Guowei Li,Qingli Yang,Jing Pan,Yanru Ji
This study aimed to investigate the antithrombotic activity of hemp seed peptides derived from trypsin hydrolysates and elucidate their molecular mechanisms using a ponatinib-induced zebrafish ( Danio rerio ) thrombosis model. Through ultrafiltration and LC–MS/MS analysis, we identified 1741 peptide sequences (< 3 kDa), with 67% distributed in the 0.5–1.5 kDa range. In vivo experiments demonstrated that 125 μg/mL hemp seed peptides significantly ameliorated ponatinib-induced thrombosis by increasing 16.55% cardiac erythrocyte staining intensity, enhancing 54.8% blood flow velocity, and restoring 11.2% intersegmental blood vessel diameters. In addition, the observed anticoagulant effect may be partially attributed to upregulated expression levels of vascular endothelial growth factor receptor 1 ( vegfr1 ) and down-regulated expression levels of cysteinyl aspartate-specific proteinase-3 ( caspase-3 ) at the mRNA level. These findings propose hemp seed peptides as novel dietary antithrombotic agents with potential applications in functional food development.
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高影响因子
危险品化学品经营许可证（带存储）