JOURNAL OF SEPARATION SCIENCE
·
2022
·Vol. 45
·期 13
·2394-2405
·
IF 3.614
Xu-Wei Qi,Yi-Ming Liu,Yi-Kao Hu,Hao Yuan,Emmanuel Ayodeji Ayeni,Xun Liao
A novel strategy of performing ligand fishing with enzyme-modified open tubular microchannel was proposed for screening bioactive components present in medicinal plants. Monoamine oxidase B was immobilized onto the surface of the microchannel for the first time to specifically extract its ligands when the plant's extracts solution flows through the channel. The thermal and the storage stability of immobilized monoamine oxidase B were significantly enhanced after immobilization. Crocin I and Ⅱ were extracted from Crocus sativus , and tiliroside was extracted from Edgeworthia gardneri . All the three compounds were inhibitors of the enzyme with the half-maximal inhibitory concentration values of 26.70 ± 0.91, 19.88 ± 2.78, and 15.65 ± 0.85 μM, respectively. The enzyme inhibition kinetics and molecular docking were investigated. This is the first report on the inhibitory effects of tiliroside and crocin Ⅱ. The novel ligand fishing method proposed in this work possesses advantages of rapidness, high efficiency, and tiny sample consumption compared to routine ligand fishing, with promising potential for screening active natural products in complex mixtures.
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高影响因子
MOLECULAR PHARMACEUTICS
·
2017
·
IF 4.44
Guo Ma,Ying Zhang,Wenyan Chen,Zhifang Tang,Xiaoming Xin,Ping Yang,Xiaoqin Liu,Weimin Cai,Ming Hu
Bilirubin-related adverse reactions (ADR, e.g., jaundice and hyperbilirubinemia) induced by herbs rich in certain polyphenolic acids are widely reported. However, the causes and the mechanisms underlying these ADR are not well understood. The purpose of this article is to determine the mechanism by which certain polyphenolic acids inhibit UGT1A1-mediated bilirubin glucuronidation, leading to jaundice or hyperbilirubinemia. We investigated in vitro inhibitory effects on bilirubin glucuronidation of salvianolic acid A (SAA), salvianolic acid B (SAB), danshensu (DSS), protocatechuic aldehyde (PA), and rosmarinic acid (RA), as well as two Salvia miltiorrhiza injections (DSI and CDI) rich in polyphenolic acids. The results showed that average formation rates of three bilirubin glucuronides displayed a significant difference (p < 0.05) and the formation of monoglucuronide was favored regardless if an inhibitor was present or not. SAA, SAB, DSI, and CDI, but not DSS, PA, and RA, significantly inhibited human UGT1A1-mediated bilirubin glucuronidation via a mixed-type inhibitory mechanism. Average IC50 values of SAA, SAB, DSI, and CDI-mediated inhibition of bilirubin glucuronidation were bilirubin concentration-dependent, and their values (against total bilirubin glucuronidation) were in the range 0.44 ± 0.02 to 0.86 ± 0.04 μg/mL (for SAA), 4.22 ± 0.30 to 12.50 ± 0.93 μg/mL (for SAB), 9.29 ± 0.76 to 18.82 ± 0.63 μg/mL (for DSI), and 9.18 ± 2.00 to 22.36 ± 1.39 μg/mL (for CDI), respectively. In conclusion, SAA and its analog SAB are the main ingredients responsible for inhibition of bilirubin glucuronidation by DSI and CDI, whose use is associated with many high bilirubin-related ADR.
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高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Yimin Yan,Yanping Hou,Zebin Yu,Lingli Tu,Shanming Qin,Danquan Lan,Shuo Chen,Jiangli Sun,Shuangfei Wang
Developing efficient and durable oxygen reduction reaction (ORR) cathodic catalysts plays an essential role in application of microbial fuel cells (MFCs). Herein, the B-doped graphene quantum dots implanted into bimetallic organic framework (BGQDs/MOF-t) are fabricated by a facile electro-deposition. Results show that, the in-situ growth of FeCoMOF on nickel foam can effectively assist construction of nanoflowers with compact connections, thus improves the conductivity. More importantly, this nano-network can serve as the template for the implantation of BGQDs through powerful interface of M-O-C bonding, avoiding π-π rearrangement and providing efficient charge transfer and abundant edge active sites. Benefitting from the enhanced electrode/electrolyte transport interface, abundant catalytic sites and low charge transfer resistance, the BGQDs/MOF-15 exhibits excellent ORR activity, superior to commercial Pt/C catalyst. In the MFC with the BGQDs/MOF-15 cathode, the maximum power density of 703.55 mW m −2 is achieved, which is 1.53 times of that of the Pt/C cathode. In addition, the BGQDs/MOF-15 cathode maintains great stability over 800 h, while that of Pt/C reduces to 61% of the initial voltage. This work opens new opportunities for developing efficient and durable MOF-derived ORR catalyst.
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高影响因子
JOURNAL OF POLYMER SCIENCE
·
2023
·Vol. 61
·期 14
·1486-1494
·
IF 3.4
Chenqi Zhao,Penghua Yan,Ruofan Liu,Wei Li,Yushun Jin,Qisong shi,Yibo Wu
The cationic polymerization of isobutene vinyl ether(IBVE) contains waters system was achieved by using triphenylmethylium tetrakis-(pentafluorophenyl)borate [Ph 3 C][B(C 6 F 5 ) 4 ], which overcame the harsh experimental requirements of traditional cationic polymerization. The influences of water content, solvent polarity, and reaction temperature on polymerization were studied. The end structure of the polymer was analyzed by 1 H-NMR. Combined with the effect of the proton trapping agent and triethylamine salt which can trap [H + ], it was found that there was a reversible equilibrium between [Ph 3 C + ] and [H + ] active species during the polymerization process. The cationic polymerizations of isobutyl vinyl ether were initiated by these two active species simultaneously. The controllable cationic polymerization of isobutyl vinyl ether was achieved by the addition of ethyl methyl carbonate and ethyl benzoate. Gaussian 09 W simulation confirms the polymerization mechanism. Graphical
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ACS Sustainable Chemistry & Engineering
·
2022
·
IF 9.224
Yafei Li,Shiqing Wang,Shunxin Fan,Babar Ali,Xiaocheng Lan,Tiefeng Wang
For the ethanolysis of biomass-derived furfuryl alcohol (FA) to value-added ethyl levulinate (EL), the development of an efficient solid acid catalyst is very important for industrial application with high FA concentration. Herein, a H-ZSM-5 catalyst with nano b-axis channels (NB-H-ZSM-5) was synthesized and used for the conversion of FA. Detailed catalyst characterization proved that the NB-H-ZSM-5 catalysts (Si/Al = 25∼125) with ∼70 nm b-axis channels were successfully prepared. The NB-H-ZSM-5 catalyst with Si/Al = 75 showed the highest activity. Its structural characteristics allowed for fast diffusion of reactants and products and full utilization of active sites, which endowed it with higher activity compared to commercial H-ZSM-5 (C-H-ZSM-5). Its moderate acidity effectively inhibited the polymerization of FA even at a high FA concentration of 20 wt %. The spent catalyst was easily regenerated by coke burn-off to give a good recyclability. Furthermore, based on the proposed reaction network, a method of physically mixing the NB-H-ZSM-5 (75) catalyst with a small amount of Amberlyst-15 was proposed to further increase the EL yield from 64.7 to 82.1% at an FA concentration of 6 wt %.
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高影响因子
CERAMICS INTERNATIONAL
·
2025
·
IF 5.6
Huilin Lun,Yi Zeng,Xiang Xiong,Houbu Li,Ziming Ye,Xiaoxu Liu
This work introduces fabricating single-phase ternary-anion carbide solid solution via solid-state diffusion of B and N. Initially, non-stoichiometric (Zr,Hf,Ti)C x with carbon vacancies was synthesized by modified spark plasma sintering. Then, using B 2 O 3 and g -C 3 N 4 as sources, (Zr 0.3 Hf 0.5 Ti 0.2 )(C 0.87 B 0.03 N 0.07 ) with face-centered-cubic structure was produced via B diffusion at 1100 °C and N diffusion at 1600 °C. Trace B (0.23 wt%) and N (0.71 wt%) in (Zr 0.3 Hf 0.5 Ti 0.2 )(C 0.87 B 0.03 N 0.07 ) was found to significantly enhance the oxidation resistance (higher onset temperature 520 ± 3 °C (particle size 2.5 ± 1.3 μm) than 475 ± 3 °C of (Zr 0.3 Hf 0.5 Ti 0.2 )C 0.85 and improve structural stability with more negative binding energy.
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高影响因子
Environmental Chemistry and Ecotoxicology
·
2025
·
IF 8.2
Huizi Zheng,Zhipeng Zhang,Guanlan Wu,Haoming Sun,Xiaolin Zhu,Jiao Qu,How Yong Ng
Nanoplastics and bisphenols (including bisphenol A and its alternatives) are emerging co-contaminants in aquatic ecosystems, yet their combined toxicological effects remain poorly understood. To address this gap, we developed a novel electrochemical biosensor using HepG2 cells and a nanocomposite of hydroxylated multi-walled carbon nanotubes, poly(diallyldimethylammonium chloride), and boron-doped graphene quantum dots (MWCNT-OH/PDDA/B-GQDs). This unique composite design, verified by density functional theory (DFT) calculations, revealed strong adsorption energies for cellular electroactive substances (xanthine: −1.12 eV; guanine: −1.56 eV), confirming the sensor's superior detection capability. The biosensor was used to assess the cytotoxicity of polystyrene nanoplastics (PS 25 nm ) and five bisphenols individually; their mixture exhibited an additive combined effect. Beyond rapid toxicity screening, we further conducted comprehensive mechanistic investigations through oxidative stress analysis, apoptosis assessment, and transcriptome sequencing. Results indicated that exposure to PS 25 nm and bisphenols, either individually or in combination, induced oxidative stress, as evidenced by decreased glutathione (GSH) levels and reduced superoxide dismutase (SOD) activity. Transcriptome analysis revealed substantial enrichment of differentially expressed genes in the pathways related to protein synthesis, oxidative stress response, and liver disease. This study not only establishes a rapid and sensitive approach for evaluating the toxicity of nanoplastics and bisphenols in aquatic environments but also provides a comprehensive understanding of the underlying mechanisms through integrated multi-level analysis, thereby providing essential mechanistic data to support their comprehensive biosafety assessment and regulatory management.
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高影响因子
ELECTROCHIMICA ACTA
·
2025
·
IF 5.6
Tie Liu
The development of magnetic carbonous composites for enhancing the performance of lithium-oxygen batteries continues to pose a significant challenge. In this study, we propose a facile approach without any template agent and surfactant to prepare a porous carbon matrix with discrete active FeCo-BN/C sites, achieving a total metal loading of ca. 4.67 at.%. This lightsome and magnetic composite exhibits a hydrangea-like hierarchical morphology and possesses key features essential for cathodic catalysis, including high catalytic activity and a large porous surface structure capable of accommodating abundant discharge products. Consequently, this interesting structure shows enhanced electrocatalytic activity and cyclic stability, surpassing that of commercial Pd/C catalyst. DFT calculations also revealed that the synergistic effect of dual-metal FeCo-N/C sites with B-doping coordination induced asymmetric charge distribution, amplifying the adsorption/desorption of oxygen intermediates. This work establishes a significant paradigm for the structural construction of hierarchical bimetal-BN/C catalysts tailored for air cathodes.
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高影响因子
TALANTA
·
2025
·
IF 6.1
Xiaoshuang Wang,Lijun Wang,Mengnan Wu,Yihao Zheng,Ruirui Wang,Tong Shao,Suyuan Zeng,Aifeng Li,Rui Li,Qiaoli Yue
A dual-mode approach was developed for dopamine (DA) assay based on boron (B) and nitrogen (N) co-doped carbon dots (B, N-CDs). This platform enabled highly sensitive and specific detection of DA in biological samples through collaborative ratio fluorometry and colorimetry. B, N-CDs were synthesized via a one-pot hydrothermal method using 3-aminobenzylboric acid (3-APBA) and proline (Pro) as precursors. B, N-CDs exhibited dark blue fluorescence under ultraviolet (UV) light excitation with a quantum yield (QY) of 0.05. In the presence of DA, the fluorescence of B, N-CDs displayed bright blue with the QY at 0.22. The original fluorescence peak intensity at 420 nm of B, N-CDs decreased, while a new peak at 465 nm increased significantly with the increasing concentration of DA. In this case, a ratio fluorometry for DA detection was constructed using B, N-CDs as fluorescence probes. On the other way, the color of B, N-CDs solution changed from colorless to brown with the addition of DA. A colorimetry for DA sensing was established based on the absorbance enhancement of B, N-CDs. There is excellent linear relationship within the concentration range of 2.5–500 μM for DA sensing with limit of detection (LOD) at 0.22 μM (ratio fluorometry) and 1.04 μM (colorimetry) and the relative standard deviations (RSD%) are 0.2170 and 0.02131, respectively. To enable the real-time visual and portable quantification of DA, two intelligent methods were explored by a program named RGB color analysis in a smartphone and machine learning. This dual-mode sensing strategy combined high sensitivity, wide linear range and ease of operation, offering a novel solution for rapid analysis of DA in complex biological substrates.
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PubMed
高影响因子
BIOMACROMOLECULES
·
2012
·
IF 5.479
Gang Liu,Chang-Ming Dong
A photoresponsive S-(o-nitrobenzyl)-l-cysteine N-carboxyanhydride (NBC-NCA) monomer was for the first time designed, and the related poly(S-(o-nitrobenzyl)-l-cysteine)-b-poly(ethylene glycol) (PNBC-b-PEO) block copolymers were synthesized from the ring-opening polymerization (ROP) of NBC-NCA in DMF solution at 25 °C. Their molecular structures, physical properties, photoresponsive self-assembly, and drug release of PNBC-b-PEO were thoroughly investigated. The β-sheet conformational PNBC block within copolymers presented a thermotropic liquid crystal phase behavior, and the crystallinity of PEO block was progressively suppressed over the PNBC composition. The characteristic absorption peaks of these copolymers at about 310 and 350 nm increased over UV irradiation time and then leveled off, indicating that the o-nitrobenzyl groups were gradually photocleaved from copolymers until the completion of photocleavage. The PNBC-b-PEO copolymers self-assembled into spherical nanoparticles in aqueous solution, presenting a photoresponsive self-assembly behavior, together with a size reduction of nanoparticles after irradiation. The anticancer drug doxorubicin can be released in a controlled manner by changing the light irradiation time, which was induced by gradually photocleaving the PNBC core of nanoparticles. This work provides a facile strategy not only for the synthesis of photoresponsive polypeptide-based block copolymers but also for the fabrication of photoresponsive nanomedicine potential for anticancer therapy.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2020
·
IF 7.489
Qiao Yan,Xiao-Yu Wang,Jiu-Ju Feng,Li-Ping Mei,Ai-Jun Wang
Water pollution caused by organic pollutants has aroused great concern to earth sustainability in the last several decades. Herein, a facile one-pot solvothermal approach was developed to construct bimetallic platinum-rhodium alloyed nano-multipods (PtRh ANMPs) in oleylamine (OAm) by adopting creatinine and cetyltrimethylammonium chloride (CTAC) as the co-structure-directing agents. The as-fabricated nanocatalyst exhibited extraordinary catalytic behaviors towards 4-nitrophenol (4-NP) and rhodamine B (RhB) reduction by sodium borohydride (NaBH 4 ). Impressively, the resultant catalyst showed highly facilitated rate constant ( k : 0.209/0.354 min −1 ) and turnover frequency (TOF: 1.0 × 10 −3 /0.44 × 10 −3 mol g −1 min −1 ) towards 4-NP and RhB reduction in comparison with commercial Pt black under the same conditions. This research offers some valuable insights for facile preparation of advanced nanostructures as efficient and recyclable catalyst in environmental remediation field.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Dandan Fang,Xiangqin Zheng,Huan Yi,Hong Dai,Zhensheng Hong,Yiru Peng,Yanyu Lin
A highly sensitive electrochemiluminescence (ECL) immunosensor was fabricated for the quantitative analysis of deoxynivalenol (DON) based on an effective all-in-one bioprobe that composed of TiO 2 -B with Fluoro-coumarin silicon phthalocyanine (F-couSiPc) and luminol. Herein, TiO 2 -B with large specific area was served as a favorable carrier, greatly increased the loading of enhancer F-couSiPc and luminophore luminol, resulting in the tremendous enhancement of ECL signal. Specifically, F-couSiPc as coreaction accelerator could facilitate the decomposition of dissolved O 2 to generate substantial reactive oxygen species (ROS) to participate in the ECL reaction of luminol. Additionally, the prepared PEDOT/HA@mp MnO 2 was employed as the sensing platform of biosensor, which can not only capture substantial antibody to improve the sensitivity, but also could expedite the electronic transmission rate to make the ECL intensity further amplified. Ultimately, the proposed immunosensor presented favorable performance for the determination of DON, such as high sensitivity, good stability, wide liner range (from 1.0*10 −6 ng/mL to 10 ng/mL) and a low detection limit of 3.3*10 -7 ng/mL.
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高影响因子
危险品化学品经营许可证（带存储）