SENSORS
·
2019
·Vol. 19
·期 23
·5152
·
IF 3.031
Qingmin Wei,Jianjuan Lin,Fa Liu,Changchun Wen,Na Li,Guobao Huang,Zhihui Luo
A surface-enhanced Raman scattering (SERS) tag is proposed for high-sensitivity detection of gibberellin A3(GA3). Silver nanoparticles (AgNPs) were synthesized using citrate reduction. 4-Mercaptobenzoic acid (MBA) was used for the Raman-labeled molecules, which were coupled to the surface of the AgNPs using sulfydryls. MBA was coated with silica using the Stöber method to prevent leakage. GA3antibodies were attached via the active functional groups N-Hydroxysuccinimide (NHS) and N-Ethyl-N’-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) to construct a novel[email protected]2SERS tags. The captured SERS substrates were fabricated through Fe3O4nanoparticles and gold nanoparticles (AuNPs) using chemical methods. These nanoparticles were characterized using ultraviolet-visible spectroscopy (UV–Vis), dynamic light scattering, Raman spectroscopy, transmission electron microscope (TEM), and X-ray diffraction (XRD). This[email protected]2SERS tags has a strong SERS signal based on characterizations via Raman spectroscopy. Based on antigen-antibody reaction, the[email protected]3O4nanoparticles can capture the GA3and[email protected]2SERS tags. Due to the increasing number of captured nanoprobes, the SERS signal from MBA was greatly enhanced, which favored the sensitive detection of GA3. The linear equation for the SERS signal was y = −13635x + 202211 (R2= 0.9867), and the limit of detection (LOD) was 10−10M. The proposed SERS tags are also applicable for the detection of other food risk factors.Keywords:surface-enhanced Raman scattering;[email protected]2core-shell nanoparticles;[email protected]3O4nanoparticle;gibberellin
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Journal of Ocean University of China
·
2019
·Vol. 18
·期 6
·1523-1531
·
IF 0.699
Shi Xiaofeng,Yan Xia,Zhang Xinmin,Ma Lizhen,Zhang Xu,Wang Chunyan,Ma Jun
Sensitivity is crucially important for surface-enhanced Raman spectroscopy (SERS) application to detect trace-level polycyclic aromatic hydrocarbons (PAHs) in the seawater. In this study, a high sensitivity three-dimensional (3-D) SERS substrate composed with syringe filter, glycidyl methacrylate-ethylene dimethacrylate (GMA-EDMA) porous material and optimal parameters (57 nm, pH 13) gold nanoparticles (AuNPs) was developed for the detection of PAHs in water. The enhancement effect and repeatability of this 3-D substrate were also explored. The Raman intensity of pyrene using 3-D SERS substrate is about 8 times higher than that of substrate only using pH 13 gold colloid solution and about 12 times higher than that of substrate using natural AuNPs and GMA-EDMA porous material, which means both the pH 13 AuNPs and the GMA-EDMA porous material are important factors for the sensitivity of this 3-D SERS substrate. Good repeatability of this optimal 3-D substrate was obtained. The relative standard deviation (RSD) is less than 8.66% on the same substrate and less than 3.69% on other different substrates. Four kinds of PAHs, i.e. , phenanthrene, pyrene, benzo(a)pyrene, benzo(k)fluoranthene and their mixture, were detected at the different concentrations. Their limits of detection (LODs) are 8.3×10 −10 (phenanthrene), 2.1×10 −10 (pyrene), 3.8×10 −10 (benzo(a)pyrene) and 1.7×10 −10 mol L −1 (benzo (k)fluoranthene), respectively. In addition, these four PAHs were also detected by fluorescence spectroscopy to evaluate the sensitivity of SERS technology using this optimal 3-D SERS substrate. The results showed that the sensitivity of SERS based on the 3-D SERS substrate even using the portable Raman system was closed to that of fluorescence spectroscopy. Therefore, the SERS technology using this optimal 3-D substrate is expected to be an in-situ method for the detection of environmental PAHs.
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ANALYST
·
2019
·Vol. 144
·期 15
·4526-4533
·
IF 4.019
Pan Li,Meihong Ge,Chentai Cao,Dongyue Lin,Liangbao Yang
Development of analytical methods allowing sensitive detection of neurotransmitters in various biofluids is vital. However, limitations of these methods include interference of impurities and stringent requirements concerning sample purity. In the current work, we developed a strategy for the rapid and sensitive analysis of dopamine (DA) in various biofluids with a smart surface-enhanced Raman spectroscopy (SERS) probe composed of magnetite Fe3O4 and Au nanoparticles (Fe3O4/Au NPs). Besides the simple and quick separation of DA from the specimen, Fe3O4 not only enabled a specific chemical interaction with DA molecules, but also acted as a SERS substrate capable of electromagnetically enhancing the Raman signal of DA. Therefore, the Fe3O4/Au NP composite with its coexisting electric-field effect and charger transfer (CT) enhancement was found to be beneficial for capturing the target molecules in biological environments and then enhancing the DA sensitivity. To understand the strong binding interaction between Fe3O4/Au and DA, X-ray photoelectron spectroscopy (XPS) was carried out, specifically to illuminate the chemical adsorption or possible CT complex. Moreover, a rapid purification strategy for further separating DA from serum was developed, and thus a high nanometer-level sensitivity was achieved. In addition, the feasibility of using Fe3O4/Au combined with the developed purification method was also verified using various tissue homogenates spiked with DA molecules. Such a nanocomposite can offer the possibility of efficiently separating DA from the complex specimen and then providing the sensitive detection of DA for various tissues. Accordingly, the smart SERS Fe3O4/Au nanocomposite probe, with its advantages of simple pre-treatment and synergetic enhanced mechanisms, shows great promise for the rapid and sensitive detection of DA in complicated specimens.
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TALANTA
·
2018
·
IF 4.244
Chunchen Zheng,Liying Zhang,Fangyuan Wang,Yueqing Cai,Shuhu Du,Zhongping Zhang
Most of efforts have been made to prepare high performance surface-enhanced Raman scattering (SERS) substrate for amplifying Raman signals. It still remains a grand challenging task in building a simple, conventional and low-cost SERS substrate with highly dense hotspots for improved sensitivity of the target analytes. Here, we report a very dexterous strategy to fabricate a distinctive SERS substrate with high density hotspots, using common adsorbent activated carbon (AC) as template to assemble silver nanoparticles (Ag NPs). It can be estimated that the enhancement effect of Ag NPs/AC composite is about 6.5-fold that of bare Ag NPs. Different from the resonant dyes, however, formaldehyde (FA) is a Raman-inactive molecule even though enhanced. Considering that, a novel method for quantitative analysis of FA using the Ag NPs/AC composite as SERS sensor has been developed, based on the catalytic effect of trace FA on the oxidation of malachite green (MG) through bromate under acidic condition. The change of MG from reduced form into oxidized leucomalachite green (LMG) results in the quench of Raman signals of MG, responding to 0.07 ppb FA that is about 2 orders of magnitude lower than the limit defined by the Nash's method as a standard procedure recommended in Europe, Japan and China. Moreover, SERS examinations of endogenous FA in human urine signify that the proposed method has high selectivity, reliability and accuracy. Thus, as-fabricated Ag NPs/AC composite is adequate as inexpensive and versatile SERS sensor utilized in the quantification of trace targets in various complicated matrices.
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Food Analytical Methods
·
2018
·Vol. 11
·期 11
·3161-3169
·
IF 2.245
Kang Yan,Li Long,Chen Wanchao,Zhang Feiyu,Du Yiping,Wu Ting
A structure of polyurethane micelle/Ag nanoparticle (Ag NP) cluster was fabricated as surface-enhanced Raman scattering (SERS) substrate for in situ extracting organic compounds on the surface of different matrixes, in which polyurethane micelles acted as the host material to capture target molecules and stabilize nanoparticle cluster. This method provided stable aqueous suspensions of Ag NP cluster due to the amphiphilic properties of polyurethane. We demonstrated SERS-based in situ detection of pesticides on vegetables and fruit skin, by simply dropping polyurethane micelle/Ag NPs substrate on the sample surface where the target molecules could be detected without a previous elution and extracting. The obtained results showed that this polyurethane micelle/Ag NP cluster substrate had excellent SERS performance for pesticide molecules with ideal stability and reproducibility. With further optimization, the limit of detection of 0.03 μg/mL acetamiprid, 0.08 μg/mL phosmet, and 0.002 μg/mL thiabendazole was obtained, respectively.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2014
·
IF 3.107
Mingxin Li,Hong Yang,Shuqun Li,Kang Zhao,Jianguo Li,Danni Jiang,Lulu Sun,Anping Deng
Phenylethanolamine A (PA) is a new kind of β-agonist, which was illegally used as a feed additive for growth promotion in China. In this study, a novel immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for the ultrasensitive and quantitative detection of phenylethanolamine A is presented. The principle of this new ICA is similar to that based on colloidal gold particles, but using AuMBA@Ag-Ab [e.g., polyclonal antibody of PA labeled Au@Ag core–shell nanoparticles (NPs) sandwiched with a Raman reporter (4-mercaptobenzoic acid, MBA)] as a probe. After ICA procedures, the specific Raman scattering intensity of MBA on the test line was measured for quantitative detection of PA. This assay was completed within 15 min. The IC50 and limit of detection (LOD) values of the ICA for PA detection were 0.06 ng mL–1 and 0.32 pg mL–1, respectively, which were 1–3 orders of magnitude lower than those obtained by other immunoassays, indicating the ultrasensitivity of this ICA. There was no cross-reactivity (CR) of the assay with another three β-agonists (ractopamine, clenbuterol, and salbutamol), suggesting high specificity of the SERS-based ICA. A spiking experiment revealed that the recoveries of PA from pig urine samples were in range of 99.9– 101.2% with relative standard deviations (RSDs) of 3.6–5.8%. The results demonstrated that this SERS-based ICA was able to quantitatively detect PA in urine samples with high sensitivity, specificity, precision, and accuracy and might be a powerful method for the analysis of other target analytes in the food area.
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TALANTA
·
2015
·
IF 3.545
Junrong Li,Guannan Zhang,Lihua Wang,Aiguo Shen,Jiming Hu
In this work, we suggest a chitosan-modified popcorn-like Au–Ag nanoparticles (CSPNPs) based assay for high sensitive detection of melamine , in which CSPNPs not only provide with an intrinsic peroxidase-like activity but also act as surface enhanced Raman scattering (SERS) substrates. CSPNPs can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 to the charge transfer complex (CTC), which contributes to a tremendous surface-enhanced resonant Raman scattering (SERRS) signals with 632.8 nm laser excitation. The target molecule melamine can generate an additional compound with H 2 O 2 , which means the available amount of H 2 O 2 for the oxidation of TMB reduced. Correspondingly, the SERRS intensity of CTC is decreased. The decreased Raman intensity is proportional to the concentration of melamine over a wide range from 10 nM to 50 μM ( R 2 =0.989), with a limit of detection (LOD) of 8.51 nM. Moreover, the proposed highly selective method is fully capable of rapid, separation-free detection of melamine in milk powder.
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Analytical Methods
·
2014
·Vol. 6
·期 15
·5925-5933
·
IF 1.938
Lei Zheng,Hui Mao,Liying Zhang,Yang Jin,Yujie Zhou,Yan Peng,Shuhu Du
The adulteration of traditional Chinese medicine (TCM) with synthetic drugs severely harms the TCM effect and public health. Thus, quick detection of synthetic drug found in TCM is crucial. Here, we report surface-enhanced Raman scattering (SERS) coupled with density functional theory (DFT) calculations for the direct identification and detection of aminopyrine (AP) in TCM using silver-coated gold nanoparticles (Au@Ag NPs). It has been demonstrated that the Raman enhancement of Au@Ag NPs to AP was stronger than that of Au, Ag and Ag@Au NPs, and there was a strong dependence of the Raman enhancement on the substrate concentration. Moreover, we obtained the DFT calculation result of AP and found that the calculation result was in good agreement with the experimental one. In addition, the characteristic peak at 999 cm−1, which was the strongest peak in the SERS spectra, was used for a quantitative evaluation of the AP level. The limit of detection (LOD) was 2.50 × 10−7 M (equal to 57.8 ng mL−1) in aqueous solution, which was in the same order of magnitude as that obtained by liquid chromatography-mass spectrometry (LC-MS), and the recovery of AP ranged from 93.9% to 101% in the spiked TCM (Shexiang Fengshi capsule) sample. Each test using the SERS sensor only needed a 10 μL sample solution and the whole test could be accomplished within ∼3 min. The SERS technique can be applied to authenticate and detect trace AP in real herbal products.
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ANALYST
·
2016
·Vol. 141
·期 19
·5511-5519
·
IF 4.033
Miao Chen,Hua Yang,Liya Rong,Xiaoqing Chen
A novel facile method for on-site determination of sulphite in wine using a gas-diffusion microfluidic paper-based analytical device (μPAD) combined with surface-enhanced Raman spectroscopy (SERS) was explored. The inexpensive and disposable μPAD was realized by sandwiching the ZnO-paper disc and integrating gas-diffusion separation and gas preconcentration on a paper-based platform. Through extensive condition optimization, the SERS signal at a shift of 620 cm−1 and the concentration of SO2 showed good linearity in the range of 5–300 μg mL−1. The linear correlation coefficient was 0.995 and the detection limit for sulphite was found to be 2 μg mL−1. Ultimately, this proposed method was applied to the determination of sulphite in wine, which can be essentially paralleled with the official method. Endowed with portability, minimal reagent consumption, and operational simplicity, this developed device would find broad utilization for on-site monitoring of sulphite.
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Siyue Xiong,Chengxuan Wang,Chushu Zhu,Peitao Dong,Xuezhong Wu
Sweat, a noninvasive metabolic product of normal physiological responses, offers valuable clinical insights into body conditions without causing harm. Key components in sweat, such as urea and glucose, are closely linked to kidney function and blood glucose levels. Portable sweat sensors, equipped with diverse sensing systems, can monitor fluctuations in urea and glucose concentrations, thus providing methods for assessing kidney function and monitoring diabetes. This study presents a flexible, portable microfluidic surface-enhanced Raman scattering (SERS) sensor designed to detect the unique fingerprint of target biomarkers. This flexible, self-adhesive microfluidic chip, constructed from modified polydimethylsiloxane, features silver nanotripods (AgNTs) with densely distributed “hotspots” created via the oblique angle deposition technique. These AgNTs act as active substrates for SERS within the microfluidic platform, enabling direct skin contact to collect, transport, store, and analyze sweat. The chip functions as a quantitative urea sensor with a limit of detection (LOD) of 10–7 M. For enhanced sensitivity for glucose detection, the SERS substrate is modified with 4-mercaptophenylboronic acid, achieving a LOD of 10–7 M. This satisfies the measurement requirements for both urea and glucose in sweat under physiological conditions. Furthermore, the one-dimensional convolutional neural network model significantly enhances the accuracy of biomarker detection, facilitating the quantitative analysis of urea and glucose. This advancement contributes to the development of a controlled, convenient, and dynamic biosensing system for personalized point-of-care testing and supports the creation of intelligent wearable and nondestructive devices.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Yilin Lu,Jie Zhong,Guohua Yao,Qing Huang
A prototype of label-free SERS sensor, based on the formation of T-Hg 2+ -T pairs and the corresponding orientation variation of DNA on Au shell surface, was developed for detection of Hg 2+ in aqueous solution quantitatively and selectively. The DNA aptamer immobilized on the surface of SiO 2 @Au core/shell nanoparticles (NPs) consisted of two segments, namely, the segment which has the consecutive thymines (T) severing as the Hg 2+ recognition elements, and the segment which contains the guanine (G) and adenine (A) bases working as the signal reporter. With interaction of Hg 2+ ions and the thymines between the adjacent single-stranded DNA (ssDNA), the DNA molecule adopted vertical orientation, resulting in increase of Raman intensity ratio I(660 cm −1 )/I(736 cm −1 ) with increase of Hg 2+ concentration, which thus allowed to measure trace amounts of Hg 2+ in aqueous solution selectively and quantitatively. Our results revealed that this label-free SERS sensor could sensitively respond to Hg 2+ ions within a wide concentration range (from 1 × 10 −8 to 1 × 10 −3 M). This work therefore demonstrates that proper design of aptamer-modified SiO 2 @Au core/shell NPs can be utilized for label-free SERS detection of heavy metal ions in the environment.
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LASER PHYSICS LETTERS
·
2018
·Vol. 15
·期 5
·055701
·
IF 2.235
Xi Liu,Meizhen Huang,Jie Chen,Lili Kong,Keihui Wang
A simple method, based on a roll-to-roll ultraviolet micro-pyramid imprinting technique and a nanoparticle self-assembling process in aqueous solution, to fabricate a large-area, flexible surface-enhanced Raman scattering (SERS) polyethylene glycol terephthalate substrate is proposed. The SERS substrate is demonstrated to be of high sensitivity. The detection concentration of Rhodamine 6G (R6G) measured by a portable Raman spectrometer is down to 10−9 mol l−1. The relative standard deviation values of different spots and different substrates are less than 13%. In addition, the feasibility for rapid detection of dye in herbal tea based on this SERS substrate and a portable Raman spectrometer is investigated. Three industrial dyes are employed to simulate the dyeing process. It is presented that R6G of 4.8\times {{10}^{-7}} g ml−1, malachite green of 10−6 g ml−1 and Auramine O of 10−6 g ml−1 in herbal tea could be detected rapidly. The experimental results imply that this method could be potentially applied in the field of dyed herbal tea detection.
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