npj Clean Water
·
2025
·Vol. 8
·期 1
·1-9
·
IF 11.4
Peng Zhaozhou,Sun Jiangyuan,Li Panpan,Li Junzhang,Shen Chen,Tian Xia,Lian Jing,Khangale Phathutshedzo,Zhang Xiangjing,Matambo Tonderayi,Hildebrandt Diane,Liu Xinying,Liu Shouxin
High levels of nitrates and nitrites not only threaten aquatic ecosystems and drinking water safety but also impair the biodegradation efficiency of industrial wastewater. In this study, micro-nano-MoS 2 -1013 (0.04 g/L) enhanced denitrification by 56.9% and 29.6% in steel pickling and meat processing wastewaters, respectively, and improved chemical oxygen demand (COD) removal by 136.7% in refinery cooling wastewater under continuous-flow conditions using a 3.5 L upflow anaerobic sludge blanket (UASB) reactor. The catalytic effect of micro-nano-MoS 2 on denitrification was achieved by stimulating an increase in the abundance of denitrification genes and the transcript levels of narL (93.82 times), narG (16.34 times), and nirK (12.27 times) within the bacterial cells, which led to an increase in the expression levels of denitrifying enzymes. These findings have significant implications for the design and optimization of biodegradation processes and bio-denitrification systems, particularly for the treatment of high-concentration nitrate wastewater.
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JOURNAL OF POLYMER RESEARCH
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2025
·Vol. 32
·期 10
·1-10
·
IF 2.8
Jiang Bihe,Zhang Zhihui,Shi Jianze,Liu Siyuan,Li Dazhen,Cao Zengwen
In this study, a mercaptan-disulfide exchange mechanism was used to modify liquid polysulfide rubber through the reaction between JLY155 and 1,6-hexanedithiol to reduce the viscosity of the liquid polysulfide. The results indicated a decrease in the viscosity and molecular weight of JLY155 and a notable improvement in the peeling strength of the prepared polysulfide sealant, demonstrating an approach to facilitating sealant preparation without sacrificing mechanical performance. Compared to JLY155, the modified liquid polysulfide rubber exhibited a reduction in viscosity, and the reduction extent could be modulated by adjusting the ratio of JLY155 and 1,6-hexanedithiol. The mechanical properties of the prepared polysulfide sealant demonstrated optimal results at a molar ratio of 4:1 between the reactants and Tris(dimethylaminomethyl)phenol (DMP30) catalyst at a loading of 0.05 wt%. The tensile strength of the prepared polysulfide sealant reached 2.38 MPa with an elongation at break of 119.68%, and the sealant exhibited excellent peel performance, undergoing substrate failure rather than adhesion failure. This was associated with a slightly lowered storage modulus (E’) in dynamic mechanical analysis. However, increasing the amount of DMP30 catalyst increased the efficiency of the thiol-disulfide bond exchange reaction and accelerated the sealant’s curing process in later stages, consequently reducing the elongation at break.
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ANGIOGENESIS
·
2025
·Vol. 29
·期 1
·1-20
·
IF 9.2
Yang Qihang,He Yuening,Wang Shuo,Li Zengting,Wang Jiaxuan,Sun Zehao,Yang Wenbo,Zhong Xiang,Peng Bo,Chen Zaozao,Gu Zhongze,Zhu Dan,Yu Tingting
Microvascular network formation is governed by a variety of factors, with interstitial flow (IF) playing a pivotal role. However, the impact of multidirectional IF (MDIF) on microvascular network development remains insufficiently explored. In this study, we developed a platform consisting of a Square chip capable of generating MDIF and a deep learning-based Vasculature-on-a-Chip Analysis Tool (VoCAT) for high-efficient analysis of vascular morphology on the chip. Using this platform, we demonstrated that microvascular networks formed on the Square chip exhibited intricate structural features with enhanced functionality. We also demonstrated its utility in modeling a tumor microenvironment with complex microvascular networks and observed enhanced tumor cell migration. This study provides the first evidence that MDIF promotes microvascular network formation, offering new perspectives for advanced in vitro vascular and disease research.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-17
·
IF 12.6
Jiang Kai,Lin Zhenyi,Cheng Mingrui,Chen Yanmin,Jiang Yiru,Xu Changhua,Zhu Lijuan,Gong Bo,Zhang Chuan,Hong Jiaxu
Dry eye disease (DED) is a prevalent ocular surface disorder characterized by chronic inflammation and oxidative stress, significantly impacting vision and quality of life. Although reactive oxygen species (ROS) serve as a key upstream regulator that initiate and perpetuate inflammatory cascades in DED, current clinical therapies predominantly target downstream inflammatory pathways, leading to suboptimal treatment outcomes. To address this limitation, we developed a novel mucin-1 (MUC1)-targeted nanotherapeutic platform (tFNA-IM) by integrating the dual antioxidant and anti-inflammatory agent itaconate (ITA) with ROS-scavenging tetrahedral framework nucleic acids (tFNA) for efficient DED treatment. The engineered tFNA-IM exhibits uniform nanostructure and optimal size distribution, enabling deep penetration into ocular tissues, while the conjugated MUC1 aptamer significantly enhances cellular internalization in human corneal epithelial cells (HCECs), as demonstrated by confocal microscopy and flow cytometry analyses. Functionally, tFNA-IM effectively neutralizes ROS, reduces apoptosis, and downregulates pro-inflammatory cytokines in vitro, demonstrating potent anti-oxidative and anti-inflammatory capabilities. In a murine DED model, tFNA-IM exhibits prolonged ocular retention and superior therapeutic efficacy, markedly improving corneal epithelial integrity and suppressing inflammatory responses. These findings establish aptamer-functionalized tFNA as a precision nanoplatform for ocular drug delivery, offering a synergistic therapeutic strategy to disrupt the ROS-inflammatory vicious cycle in DED. Graphical MUC1-aptamer–guided tetrahedral DNA (tFNA-IM) delivers itaconate to the cornea, simultaneously scavenging ROS and suppressing cytokine cascades to break the ROS–inflammation loop and restore epithelial integrity.
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IONICS
·
2025
·
IF 2.6
Xiong Zhen-Yu,Wang Guan-Hua,Wang Hong-Yu,Li Peng-Yuan,Sun Yang,Ma Yu-Han,Guo Tao,Shi Fa-Nian,Zhang Yu-Hang
Polyethylene oxide (PEO) is regarded as the most promising candidate for the next generation solid polymer lithium metal batteries (LMBs). However, it suffers from low mechanical strength and limited electrochemical stability window (ESW), which restricts its application in high energy density LMBs. In this work, polycaprolactone (PCL) is chosen as the main matrix to fabricate a blending polymer-based electrolyte with PEO, which shows an improved electrochemical performance with a high lithium ion transference number of 0.65. The improvement can be attributed to the competition between ester and ether groups of different polymer chains, which can both interact with lithium ions to release more free cations. Moreover, the high-voltage resistance of PCL can broaden the electrochemical stability window to 4.8 V of PCL/PEO solid electrolyte to match LiNi 0.8 Co 0.1 Mn 0.1 O 2 (NCM811) cathode, and its high-temperature resistance ensure a satisfying dimensional stability for the assembled NCM811||Li cell to operate at 55 ℃, with a high initial specific capacity of 210 mAh/g and a retention of 95% within 100 cycles. Compared to other blending systems, the PCL/PEO blend exhibits superior compatibility, mechanical properties, and electrochemical stability, offering a promising strategy for the commercialization of high-voltage, safe, and high-energy-density solid-state lithium metal batteries operable at elevated temperatures.
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JOURNAL OF MOLECULAR HISTOLOGY
·
2025
·Vol. 56
·期 6
·1-14
·
IF 2.2
Wang Pu,Sun Congcong,Shi Chao,Gao Yong,Luan Chunyu,Yan Chao
Osteoarthritis (OA) is a degenerative joint disease characterized by pathological changes such as articular cartilage degeneration and bone hyperplasia. Mitochondrial dysfunction in chondrocytes has been identified as a critical factor contributing to the progression of OA. Although Evodiamine (Evo) has been demonstrated effeicacy in inhibiting inflammatory responses and matrix degradation in OA chondrocytes, its effects and underlying mechanisms regarding mitochondrial dysfunction in these cells remain to be elucidated. IL-1β was utilized to stimulate chondrocytes in order to establish an in vitro OA model. Subsequently, the proliferation, lactate dehydrogenase (LDH) release, and apoptosis of chondrocytes were systematically evaluated. Mitochondrial function in chondrocytes was evaluated by quantifying of ATP content, ROS level, mitochondrial DNA (mtDNA) copy number, activities of mitochondrial respiratory chain Complexes I and III, as well as mitochondrial membrane potential change. Furthermore, the protein expression levels of the SIRT1/PGC-1α signaling pathway were examined, and an intervention involving the SIRT1 inhibitor EX527 was conducted to elucidate the potential mechanisms underlying the effects of Evo on chondrocytes. Evo treatment significantly elevated the proliferation activity of IL-1β-stimulated chondrocytes, inhibited LDH release and apoptosis, increased mtDNA copy number and ATP content, enhanced the enzymatic activities of Complexes I and III, and suppressed ROS production as well as mitochondrial membrane potential loss. Furthermore, Evo treatment activated the SIRT1/PGC-1α signaling pathway. However, the addition of the SIRT1 inhibitor EX-527 partially attenuated the protective effects of Evo against IL-1β-induced chondrocyte injury by exacerbating mitochondrial dysfunction. Evo promotes mitochondrial biosynthesis, reduces ROS production and improves mitochondrial function by activating SIRT1/PGC-1α pathway, thereby inhibiting IL-1β-induced chondrocyte injury.
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Microbial Cell Factories
·
2020
·Vol. 19
·期 1
·1-11
·
IF 4.187
Xiong Liang-Bin,Liu Hao-Hao,Zhao Ming,Liu Yong-Jun,Song Lu,Xie Zhi-Yong,Xu Yi-Xin,Wang Feng-Qing,Wei Dong-Zhi
Background The bioconversion of phytosterols into high value-added steroidal intermediates, including the 9 α -hydroxy-4-androstene-3,17-dione (9-OHAD) and 22-hydroxy-23,24-bisnorchol-4-ene-3-one (4-HBC), is the cornerstone in steroid pharmaceutical industry. However, the low transportation efficiency of hydrophobic substrates into mycobacterial cells severely limits the transformation. In this study, a robust and stable modification of the cell wall in M. neoaurum strain strikingly enhanced the cell permeability for the high production of steroids. Results The deletion of the nonessential kasB , encoding a β-ketoacyl-acyl carrier protein synthase, led to a disturbed proportion of mycolic acids (MAs), which is one of the most important components in the cell wall of Mycobacterium neoaurum ATCC 25795. The determination of cell permeability displayed about two times improvement in the kasB -deficient strain than that of the wild type M. neoaurum . Thus, the deficiency of kasB in the 9-OHAD-producing strain resulted in a significant increase of 137.7% in the yield of 9 α -hydroxy-4-androstene-3,17-dione (9-OHAD). Ultimately, the 9-OHAD productivity in an industrial used resting cell system was reached 0.1135 g/L/h (10.9 g/L 9-OHAD from 20 g/L phytosterol) and the conversion time was shortened by 33%. In addition, a similar self-enhancement effect (34.5%) was realized in the 22-hydroxy-23,24-bisnorchol-4-ene-3-one (4-HBC) producing strain. Conclusions The modification of kasB resulted in a meaningful change in the cell wall mycolic acids. Deletion of the kasB gene remarkably improved the cell permeability, leading to a self-enhancement of the steroidal intermediate conversion. The results showed a high efficiency and feasibility of this construction strategy.
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JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2018
·Vol. 55
·期 8
·3016-3025
·
IF 1.797
Li Tingting,Yang Bing,Li Xuepeng,Li Jianrong,Zhao Guohua,Kan Jianquan
The spoilage of aquatic products is mainly caused by the bacterial growth, and the specific spoilage organism (SSO) plays an important role. Quorum sensing (QS) is a microbial cell–cell communication system which is coordinated with the population density, and is controlled by N -acyl-homoserine lactone (AHLs) as the Gram-negative bacteria communication signals. In this study, the SSO was Pseudomonas fluorescens (PF-04), isolated from the turbot ( Scophthalmus maximus L. ) during aerobically refrigerated storage. The supernatant extract of PF-04 tested the AHLs activities utilizing biosensor Chromobacterium violaceum CV026. AHL production was influenced by the environment temperature, and AHL production reduced obviously at 10 °C compare with 25 °C. In Luria-Bertani (LB) supplemented with 0.5–1.0% NaCl, AHL production reached the maximum. The AHL production was also regulated by pH of culture medium, acidic condition was conducive to persistent existence of the AHL molecules, but the alkaline environment would cause chemically unstable of AHL molecules. QS system in P. flurosecens played an imperative role in biofilm formation, protease and siderophore production. AHLs could regulate above three factors in PF-04. In summary, this study showed that (1) the influence of different environmental conditions (temperature, NaCl and pH) on AHL production revealed the correlation of QS in foods and (2) that proved the effect of external AHLs to regulate the biofilm formation, protease and siderophore production in PF-04.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2017
·Vol. 15
·期 1
·1-18
·
IF 4.946
Wang Yuan,Zhang Xue-Yin,Luo Yan-Ling,Xu Feng,Chen Ya-Shao,Su Yu-Yu
Background Stimuli-responsive polymer materials are a new kind of intelligent materials based on the concept of bionics, which exhibits more significant changes in physicochemical properties upon triggered by tiny environment stimuli, hence providing a good carrier platform for antitumor drug delivery. Results Dual stimuli-responsive Fe 3 O 4 graft poly(acrylic acid)- block -poly(2-methacryloyloxyethyl ferrocenecarboxylate) block copolymers (Fe 3 O 4 - g -PAA- b -PMAEFC) were engineered and synthesized through a two-step sequential reversible addition-fragmentation chain transfer polymerization route. The characterization was performed by FTIR, 1 H NMR, SEC , XRD and TGA techniques. The self-assembly behavior in aqueous solution upon triggered by pH, magnetic and redox stimuli was investigated via zeta potentials, vibration sample magnetometer, cyclic voltammetry, fluorescent spectrometry, dynamic light scattering, XPS, TEM and SEM measurements. The experimental results indicated that the Fe 3 O 4 - g -PAA- b -PMAEFC copolymer materials could spontaneously assemble into hybrid magnetic copolymer micromicelles with core–shell structure, and exhibited superparamagnetism, redox and pH stimuli-responsive features. The hybrid copolymer micromicelles were stable and nontoxic, and could entrap hydrophobic anticancer drug, which was in turn swiftly and effectively delivered from the drug-loaded micromicelles at special microenvironments such as acidic pH and high reactive oxygen species. Conclusion This class of stimuli-responsive copolymer materials is expected to find wide applications in medical science and biology, etc., especially in drug delivery system.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-10
·
IF 14.7
Zhang Xun,Feng Ximin,Guo Wenqi,Zhang Chengjian,Zhang Xinghong
Polyvinyl chloride (PVC) is the world’s third-most widely manufactured thermoplastic, but has the lowest recycling rate. The development of PVC-like plastics that can be depolymerized back to monomer contributes to a circular plastic economy, but has not been accessed. Here, we develop a series of chemically recyclable plastics from the reversible copolymerization of cyclic anhydride with chloral. The copolymerization is highly efficient through the anionic or cationic mechanism under mild conditions, yielding polyesters with tunable structure and properties from multiple commercial monomers. Notably, these polyesters manifest mechanical properties comparable to PVC and polystyrene. Meanwhile, such polyesters are flame-retardant like PVC due to high chloride content. Of significance, these polyesters can be depolymerized back to starting monomers at high temperatures owing to the reversibility of the copolymerization, leading to a circular economy. Overall, the readily available monomers, simple synthesis, advantageous performance, and practical recyclability make the polymers promising for applications.
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Waste and Biomass Valorization
·
2024
·
IF 2.6
Luo Bo,Liu Huiyu,Shan Rui,Zhang Jun,Yuan Haoran,Chen Yong
Purpose This work aims to investigate the thermal behavior and kinetic characteristics of polyethylene (PE) pyrolysis over Cu/Ce modified ZSM-5. The influences of metal types, particles size of active center and interaction of Cu–Ce species on products distribution are elucidated. Methods Metal-modified ZSM-5 catalysts were prepared by negative pressure impregnation method. Catalytic fast pyrolysis and thermogravimetric experiments were conducted using Py–GC/MS and TG/MS for the qualitative and quantitative analysis of pyrolysis products. Results Morphology characterization indicated the interaction of Cu–Ce species facilitated the regulation of active center size into a nanoparticle scale. The final products were restricted to a carbon number of less than 9 over CuCe/ZSM-5. Cu–Ce modification increased the aliphatic content from 58.5 to 76.1%. Kinetic analysis revealed a remarkable reduction in activation energies during both the intermediate and final stages, where an average decrease of 30% was achieved. Conclusion The synergistic effects of Cu 0 and CeO x facilitated the formation of more short chain olefins by enhancing C–C bonds cleavage activity. Undesired polycyclic aromatic hydrocarbons (PAHs) were avoided by the optimal steric hindrance of CuCe/ZSM-5. Graphical Abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 409
·期 29
·6821-6829
·
IF 3.431
Liu Misha,Xu Jinjin,Yang Fan,Gu Yifan,Chen Huan,Wang Ying,Li Fengting
Detection of DNA damage caused by ∙OH or radiation has led to rapidly growing interest in the fields of drug development, biochemistry, clinic diagnostics, and environmental evaluation. Electrochemical methods have been applied for DNA damage detection because of their fast and sensitive response. However, most of the electrochemical methods for DNA damage detection commonly require immobilization of the strands on the electrode surface. In the present work, sensitive electrochemical monitoring of DNA damage was realized successfully on the basis of in situ DNA chain growth by use of a hybridization chain reaction (HCR) technique. By use of [Ru(NH 3 ) 6 ] 3+ as the signal probe and the Fenton reaction as the ∙OH generator, ultrasensitive detection of DNA damage induced by ∙OH was realized successfully through differential pulse voltammetry with a linear relationship of ∙OH concentration from 15 to 750 pM and a detection limit of 12 pM. Furthermore, environmental DNA-damaging UV light was tested as the lesion source to demonstrate the practicability and reliability of the proposed HCR-based amplified signal method for DNA damage detection. By integrating the HCR technique with an electrochemical method, we provide a promising alternative approach to extend the applications of electrochemical methods in bioanalytical detection of DNA damage.
Sensitive electrochemical monitoring of DNA damage has been realized successfully on the basis of in situ DNA chain growth by use of hybridization chain reaction (HCR). Detection of DNA damage caused by ∙OH was realized successfully through differential pulse voltammetry with a linear relationship of ∙OH concentration from 15 to 750 pM and a detection limit of 12 pM
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危险品化学品经营许可证（带存储）