Molecular Catalysis
·
2022
·
IF 5.089
Ying Zhang,Zhi-Wen Duan,Han-Yu Liu,Feng Qian,Pu Wang
Microbial whole-cell catalysis has been a popular method for chiral alcohols preparation. In this study, Cyberlindnera saturnus ZJPH1807 was successfully employed as a biocatalyst to reduce 2-chloro-1-(2,4-dichlorophenyl)ethanone (CPE) to ( R )-2-chloro-1-(2,4-dichlorophenyl)ethanol (( R )-CPEO), a key chiral intermediate of antifungal drug miconazole. In order to further improve the bioprocess yield, natural deep eutectic solvent and cyclodextrin were introduced into the reaction system as additives. It was found that carnitine/trehalose (C/Tre, 1:1 mol/mol) could increase the permeability of cell membrane appropriately and improve the mass transfer rate. And the addition of methyl-β-cyclodextrin (MCD) could improve the solubility of CPE in buffer solution, simultaneously promoting cofactor recycling. Furthermore, MCD and C/Tre exerted a synergistic effect on the acceleration of CPE bioreduction, producing 91.0% yield in 1.5 h at 35.8 mM CPE in a C/Tre-MCD-containing system, with a 12.4-fold increase in productivity compared to neat buffer solution only. When the substrate loading was increased to 67.1 mM, the yield could also achieve 84.2%. The established bioreduction process was also feasible at 100 mL preparation scale with a 88.5% ( R )-CPEO yield. The integration of C/Tre and MCD can significantly improve catalytic efficiency for hydrophobic substrates and be applied in biocatalysis.
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Experimental and Therapeutic Medicine
·
2019
·Vol. 18
·期 4
·2924-2932
·
IF 1.448
Jingjing Wang,Yongzhong Ning,Shu Li,Yun Wang,Jinhua Liang,Chunming Jin,Hairun Yan,Yongcun Huang
Acinetobacter baumannii is an important cause of hospital‑acquired, multidrug‑resistant (MDR) infections occurring worldwide. Anti‑microbial combination regimens may be the only feasible treatment option for affected patients. In the present study, the efficacy of the combined therapy of meropenem with colistin, ampicillin‑sulbactam, tazobactam and vancomycin against clinical strains of MDR A. baumannii was determined. Anti‑microbial susceptibility testing was performed and resistance genes were characterized by a multiplex polymerase chain reaction (PCR)‑reverse line blot assay. The genetic background of New Delhi metallo‑β‑lactamase 1 (NDM‑1) was analysed by primer walking. The presence of NDM‑1 was detected using the modified Hodge test and the EDTA‑combined disk test. To screen for synergistic drug effects, the fractional inhibitory concentration index was calculated using a checkerboard assay. The results of the PCR as well as the sequence analyses suggested that NDM‑1 was located downstream of the ISAba125 element. In addition, a synergistic effect was determined for meropenem + vancomycin, meropenem + tazobactam and meropenem + ampicillin + sulbactam in two strains each, and in four strains for meropenem + colistin. A total of five A. baumannii strains with resistance to numerous antibiotics and carrying numerous resistance genes were identified. In the strains of A. baumannii, the NDM‑1 gene was integrated in a transposon structure with a copy of the ISAba125 insertion sequence. However, the genetic background was not identical among the different species and strains. The genetic variability of NDM‑1 may facilitate the rapid dissemination of this gene. In conclusion, meropenem may enhance the efficacy of antibiotics in A. baumannii strains with NDM‑1‑associated MDR.
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高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2019
·
IF 3.375
Changfa Xiao,Long Jiang,Yi Dan
The unsaturated linear (UL) copolymer of methyl acrylate (MA), 1-octene, and allyl glycidyl ether (AGE) and the long-chain branch (LCB) copolymer of MA, 1-octene, and 1,7-octadiene were synthesized, respectively, via AlCl3-catalyzed radical copolymerization, and then both UL and LCB copolymers were vulcanized using dicumyl peroxide (DCP) curing system. For UL copolymer, cross-linking heat first increases with DCP dosages and then decreases when DCP dosages increases further to 2.5, that is quite reasonable to relate with cross-link and degradation reactions during vulcanization. However, the reaction heat flow curves of DCP-LCB curing system with DCP dosages ranging from 1.5 phr to 3.5 phr exhibit a bimodal-like “crosslinking” endotherm, suggesting a two-stage reaction of intramolecular cross-linking and intermolecular cross-linking. Results from dynamic mechanical analysis and tensile tests confirm that LCB copolymers are propitious to form effective networks at low DCP dosages and to keep the vulcanized topological structure with redundant DCP.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2023
·
IF 7.2
Ran Li,Hao Shi,Jinlin Song,Zihong Dong,Tao Wan,Hua Dong
The introduction of magnetism into a catalyst can greatly optimize its separation performance. In the present work, a kind of magnetically separable catalysts for promoting NaBH 4 hydrolysis have been fabricated by anchoring cobalt nanoparticles on magnetic dendritic KCC-1 nanospheres composed of magnetic Fe 3 O 4 core and fibrous shell. The fabricated catalysts were characterized with various characterization methods, including absorption spectroscopy (AAS), scanning electron microscopy (SEM), high-resolution transmission electronic microscopy (HRTEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometry (VSM), and Fourier transform infrared (FT-IR), etc. This kind of catalysts exhibit high catalytic activity for promoting the hydrolysis of NaBH 4 under alkaline conditions, giving a hydrogen generation rate and activation energy of 3.83 L min −1 g Co −1 (30 °C) and 53.63 kJ mol −1 , respectively. After used for 5 cycles, the catalyst showed 36.5% catalytic activity reserved. Most importantly, the magnetism of the catalyst made it easily separated and recycled from the solution after the reaction completed. The development of this kind of catalysts could provide a promising option for catalyzing NaBH 4 hydrolysis for portable hydrogen production from.
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Polymer Chemistry
·
2021
·Vol. 12
·期 16
·2439-2446
·
IF 5.582
Junle Zhang,Mengya Li,Yanjie He,Xiaomeng Zhang,Zhe Cui,Peng Fu,Minying Liu,Xiaoguang Qiao,Qingxiang Zhao,Xinchang Pang
Gold (Au) nanocrystals were recognized to be a remarkable photocatalyst because of their strong localized surface plasmon resonance (LSPR). Here Au nanocrystals were exploited as plasmonic photocatalysts for photo-regulated reversible addition–fragmentation chain-transfer (RAFT) polymerization. In addition to the well-controlled RAFT polymerization process under a broadband of light (from visible to the near-infrared region), the dimension (0-D nanospheres and 1-D nanorods) and aspect ratio (length/width) of the nanocrystals exhibited a remarkable effect on the polymerization rate under different light sources. The reduction of plasmon damping leads to a relatively long-lived region of high electron density on the surface of the Au nanorods, which can accelerate charge transfer and induce a higher polymerization rate. Moreover, the aspect ratio related to the longitudinal absorption band of the Au nanorods may provide more flexible choices for advanced macromolecular photosynthesis for potential in biomedical and industrial applications.
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高影响因子
CERAMICS INTERNATIONAL
·
2019
·
IF 3.45
Cheng Meng,Wenqi Li,Chunrong Ren,Junchuan Zhao
Powellite ceramic represent one candidate to immobilize minor actinides and Mo from reprocessed UMo nuclear fuel. In this work, the Ca 1-x Li x/2 Ce x/2 MoO 4 (0 ≤ x ≤ 1) series is prepared via a solid-state reaction using Ce 3+ as trivalent minor actinide (Am 3+ ) surrogate, with structure/microstructure characterized by XRD , XPS, HRTEM , and SEM as well. The Ca 1-x Li x/2 Ce x/2 MoO 4 (0 ≤ x ≤ 1) compositions crystallize in tetragonal and are isostructural with scheelite. Rietveld refinements show that Ce 3+ and Li + simultaneously enter into the eight-fold coordinated Ca site of powellite crystal. The chemical durability of powellite phases is evaluated by the ASTM C1285-14 product consistency test method B. The leaching behaviours of Ce and Mo are accordance with the interfacial dissolution-reprecipitation mechanism. For all the Ca 1-x Li x/2 Ce x/2 MoO 4 (0 ≤ x ≤ 1) ceramics, 7-days NL Ce and NL Mo are found to be in the order of 10 −3 -10 −5 g·m −2 and 10 −2 -10 −4 g·m −2 respectively, which exhibit the great retention of Ce and Mo. Interestingly, the values of 7-days NL Ce and NL Mo are predominantly controlled by the distortion of MoO 4 tetrahedra and disordered arrangements of Ce 3+ and Li + . Thus, our initial understanding on the structure and chemical durability relation will provide insight to design new waste forms for the Mo-rich HLW condition.
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XENOBIOTICA
·
2021
·
IF 1.908
Weibin Xiao,Zhirong Deng,Chongfa Lai,Haoyang Lu,Mutu Huang,Yuguan Wen,Lei Shi
l-tetrahydropalmatine (l-THP) is mainly metabolised by CYP450 enzymes.This study was to investigate the possible effect of co-administered CYP inhibitors on the pharmacokinetics of l-THP and its metabolites in rats.An established LC-MS/MS method has been applied for the evaluation of drug-drug interaction between l-THP and CYP inhibitors. Following the administration of CYP inhibitors, a single dose of l-THP (9 mg/kg) was orally administrated.With regard to l-THP, the AUC0–48 were significantly increased by 4.3, 3.79, and 11.39 folds, and Cmax were increased by 4.74, 3.64, and 2.76 folds in the ketoconazole group (KET), quinidine group (QD), and 1-aminobenzotriazole group (ABT), respectively. KET and QD both significantly increased the AUC0–48 of 2-DM and 2-DM-Glu by 1.38 ∼ 2.43 times, while Cmax was significantly decreased by 41.3 and 78.0% in the ABT group, respectively. The Cmax of 3-DM was reduced by 51.38, 48.02, and 63.31% after pre-treatment with KET, QD, and ABT, respectively, and Cmax of 3-DM-Glu decreased correspondingly by 29.6, 22.1, and 58.0%.Results indicated that CYP inhibitors could markedly influence the systemic level of l-THP and its metabolites. To guarantee the safe use of l-THP, attention should be paid when l-THP was co-administered with CYP inhibitors, particularly with CYP3A4 and 2D6 inhibitors.
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PubMed
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2023
·
IF 6.5
Yunshuai Huang,Jiawei Song,Qixian Hao,Changling Mou,Hongming Wu,Fulin Zhang,Ziyan Zhu,Ping Wang,Tengfei Ma,Kai Fu,Yaping Chen,Thanhliem Nguyen,Shijia Liu,Ling Jiang,Jianmin Wan
Seed dormancy is a critical trait that enhances plant survival by preventing seed germination at the wrong time or under unsuitable conditions. Lack of seed dormancy in rice can lead to pre-harvest sprouting on mother plants leading to reduced yield and seed quality. Although some genes have been identified, knowledge of regulation of seed dormancy is limited. Here, we characterized a weak seed dormancy mutant named weak seed dormancy 1 ( wsd1 ) that showed a higher seed germination percentage than the wild-type following the harvest ripeness. We cloned the WSD1 encoding an aminotransferase protein using a MutMap approach. WSD1 was stably expressed after imbibition and its protein was localized in the endoplasm reticulum. A widely targeted metabolomics assay and amino acid analysis showed that WSD1 had a role in regulating homeostasis of amino acids. PAC treatment and RNA-seq analysis showed that WSD1 regulates seed dormancy by involvement in the GA biosynthesis pathway. GA 1 content and expression of GA biosynthesis-related genes were increased in the wsd1 mutant compared with the wild-type. The wsd1 mutant had reduced sensitivity to ABA. Our overall results indicated that WSD1 regulates seed dormancy by balancing the ABA and GA pathways.
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高影响因子
Theranostics
·
2019
·
IF 8.063
Xu-Qi Kang,Gao-Feng Shu,Sai-Ping Jiang,Xiao-Lin Xu,Jing Qi,Fei-Yang Jin,Di Liu,Yong-Hong Xiao,Xiao-Yang Lu,Yong-Zhong Du
The prevalence of antibiotic resistance and lack of alternative drugs have posed an increasing threat to public health. Here, we prepared β-Ga 2 O 3 :Cr 3+ nanoparticles modified with ICAM1-antibody-conjugated TPGS (I-TPGS/Ga 2 O 3 ) as a novel antibiotic carrier for the treatment of drug-resistant infections. Methods : I-TPGS/Ga 2 O 3 were firstly characterized by measuring particle size, morphology, crystal structure, drug loading capacity, and in vitro drug release behaviors. The in vitro antibacterial activities of I-TPGS/Ga 2 O 3 /TIG were evaluated using standard and drug-resistant bacteria. The internalization of I-TPGS/Ga 2 O 3 was observed by fluorescence confocal imaging, and the expression levels of the efflux pump genes of TRKP were analyzed by real-time RT-PCR. In vitro cellular uptake and in vivo biodistribution study were performed to investigate the targeting specificity of I-TPGS/Ga 2 O 3 using HUEVC and acute pneumonia mice, respectively. The in vivo anti-infective efficacy and biosafety of I-TPGS/Ga 2 O 3 /TIG were finally evaluated using acute pneumonia mice. Results : It was found that TPGS could down-regulate the over-expression of the efflux pump genes, thus decreasing the efflux pump activity of bacteria. I-TPGS/Ga 2 O 3 with small particle size and uniform distribution facilitated their internalization in bacteria, and the TPGS modification resulted in a significant reduction in the efflux of loaded antibiotics. These properties rendered the encapsulated tigecycline to exert a stronger antibacterial activity both in vitro and in vivo . Additionally, targeted delivery of I-TPGS/Ga 2 O 3 mediated by ICAM1 antibodies contributed to a safe and effective therapy. Conclusion : It is of great value to apply I-TPGS/Ga 2 O 3 as a novel and effective antibiotic delivery system for the treatment of drug-resistant infections.
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高影响因子
FUEL
·
2025
·
IF 7.5
Yanxin Zhang,Xuesi Hong,Yuzhu Shi,Duo Xu,Yueqin Song,Xiaolong Zhou,Jin An Wang
The present study investigated a novel bimetallic Pt-Mo catalyst encapsulated within Silicalite-1 zeolite for propane dehydrogenation (PDH). The catalyst was synthesized via in situ hydrothermal crystallization, followed by direct high-temperature H 2 reduction without conventional calcination, leading to uniform encapsulation of Pt nanoparticles within the zeolite framework and simultaneous removal of the template and organic ligands. PDH performance was evaluated in a continuous-flow fixed-bed microreactor. Comprehensive characterizations (XPS, CO-DRIFTS, NH 3 -TPD, Raman, etc.) reveal that Mo incorporation in 0.4Pt@S-1 catalyst significantly enhances propane conversion while maintaining high propylene selectivity. Moreover, the bimetallic 0.4PtXMo@S-1 catalyst with 0.1 % Mo doping achieved an outstanding performance at 550 °C and a WHSV of 5.49 h −1 (propane conversion: 43 %, propylene selectivity: 97 %, propylene formation rate: 16.27 mol C3H6 ·g Pt -1 ·h −1 ). The prepared catalyst possessed favorable stability and regeneration performance, showing a deactivation rate of only 0.001454 h −1 after four reaction-regeneration cycles. This enhancement is attributed to synergistic effects arising from Mo incorporation, which promotes Pt nanoparticle dispersion, modulates Brønsted /Lewis acidity, and facilitates interfacial electron transfer between Pt and Mo species. These findings highlight the role of Pt-Mo interactions within zeolite frameworks, providing new insights for designing efficient and stable PDH catalysts.
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产品关联
DOI
高影响因子
Frontiers in Immunology
·
2025
·
IF 5.9
Dong-Ni Pei,Yang Song,Ying-Xia Zhou,Bo Shu,Shao-wei Huang,Fa-Zhao Li,Wei-Dong Dai,Bao-Ye Sun
Purpose Conventional type 1 dendritic cells (cDC1s) mastermind anti-cancer immunity and play a pivotal role in determining the efficacy of cancer immunotherapies. In this study, we sought to decipher the dynamic changes in the tumor immune landscape during intrahepatic cholangiocarcinoma (iCCA) development and harness the therapeutic potential of targeting cDC1s for cancer therapy. Methods We constructed spontaneous murine iCCAs via hydrodynamic tail vein injection (HDTVi) of plasmids encoding AKT/YAP. To characterize tumor-infiltrating immune cell populations throughout iCCA carcinogenesis and progression, we performed time-of-flight mass cytometry (CyTOF). To expand and activate cDC1s, we combined Flt3L with poly I:C (FL-pIC) therapy and assessed its therapeutic efficacy in both AKT/YAP-induced iCCAs and a subcutaneous tumor injection model. Flow cytometric analyses were used to evaluate intra-tumoral infiltration levels of cDCs and CD8 + T cells. Results CyTOF analysis revealed the progressive formation of an immunosuppressive tumor microenvironment as iCCA advances. Crucially, infiltration of cDC1s dramatically decreases in advanced iCCAs compared to early-stage tumors. Combined FL-pIC therapy preferentially expanded CD103 + cDC1s, powerfully inhibiting tumorigenesis in AKT/YAP-driven murine iCCAs and sensitizing these tumors to anti-PD-1 therapy. Moreover, FL-pIC therapy markedly suppressed the growth of established mIC-23 subcutaneous tumors. Conclusions Our findings demonstrate that recruiting and activating intra-tumoral cDC1s is feasible and essential for driving anti-tumor CD8 + T cell immune responses and enhancing anti-PD-1 therapeutic effectiveness in iCCA.
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PubMed
高影响因子
JOURNAL OF ENTOMOLOGICAL SCIENCE
·
2024
·
IF 0.7
Gao Shanshan,Li Dongyu,Zhou Can,Zhu Min,Zhang Yonglei,Li Ruimin,Zhang Kunpeng
Evidence is accumulating that insect epsilon class glutathione S-transferases (GSTs) play an important role in the resistance to xenobiotics such as insecticides. A Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) epsilon class GST gene, TcGSTe17 , was previously found to be significantly induced following exposure to the plant derivative eucalyptol. However, whether TcGSTe17 is involved in resistance to eucalyptol remains unclear. In this study, TcGSTe17 was cloned from the T. castaneum . Development stage expression profiling revealed that TcGSTe17 was expressed at all development stages of T. castaneum but was highly expressed in late-stage larvae. Subsequent expression profiling of tissues showed that TcGSTe17 was highly expressed in the gut of larvae and the fat body of adults, and also revealed gender-specific expression patterns. In addition, the transcripts of TcGSTe17 were significantly increased following exposure to eucalyptol, and RNAi increased the susceptibility of the beetles to eucalyptol. Collectively, these results suggest that TcGSTe17 contributes to the susceptibility of T. castaneum to eucalyptol. These findings provide new data for the prevention of pest resistance.
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