JOURNAL OF MOLECULAR LIQUIDS
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2024
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IF 5.3
Yanrong Cheng,Chunyang Liu,Xia Guo
An aqueous two-phase system (ATPS) has been considered a green separation system. How to improve the separation efficiency and selectivity of ATPS has always been a widely concerned issue. However, so far, the construction of selective ATPS still remains a challenging task. Calixarenes are the third generation of supramolecular compounds. They exhibit significant advantages in recognizing small molecules. In the present study, calixarene was used for the first time to prepare selective ATPSs, which are sulfonatocalix[4]arene (SC[4])/1-decyl (or hexyl)-3-methylimidazolium bromide ([C n mim]Br)/trisodium citrate (Na 3 C 6 H 5 O 7 ) ATPS and SC[4]/1-decyl-3-methylimidazolium bromide ([C 10 mim]Br) ATPS. Ten aromatic molecules, including theobromine, syringic acid, vanillin, vanillic acid, theophylline, cephalexin, L-tyrosine, L-phenylalanine, S-mandelic acid, and L-tryptophan, were selected as model extracts to evaluate the selectivity of these SC[4]-based ATPSs. The results showed that among these 10 extracts, the SC[4]/[C n mim]Br/Na 3 C 6 H 5 O 7 ATPS has high selectivity toward vanillin, and the SC[4]/[C 10 mim]Br ATPS has high selectivity toward L-phenylalanine. Specifically, in the SC[4]/[C n mim]Br/Na 3 C 6 H 5 O 7 ATPS, the partition coefficient of vanillin can reach 315.7, while the partition coefficients of the other 9 extracts are around 2.4–23.1 In the SC[4]/[C 10 mim]Br ATPS, the partition coefficient of L-phenylalanine is much higher than 1 (i.e., 4.38–5.55), while the partition coefficients of the other extracts are below or around 1. The 1 H NMR spectra and response surface plots based on the Box-Behnken design further demonstrate the role of [C n mim]Br-SC[4] complex in the selectivity of these ATPSs.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2025
·
IF 3.1
Lin Chen,Han Huang,Xiali Liu,Peijing Zhao,Biao Zhou
A novel oxyphenisatin analogue was identified in a type of jelly candy during routine inspections of food products marketed for weight-loss purposes. Through analysis utilizing ultra-high-performance quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap-HRMS), the fragment ions at m/z 258 and 195 observed in the MS/MS experiments were found to be consistent with those of 4-Chloro-oxyphenisatin diacetate. It was inferred that the unknown compound is likely a derivative of 4-Chloro-oxyphenisatin diacetate. The candy was separated and purified by column chromatography, and the purified compound was determined to be 96.4 % by high-performance liquid chromatography (HPLC). Subsequently, the structure was confirmed through nuclear magnetic resonance (NMR) spectroscopy. Based on the data, it was concluded that the structure of the unknown compound involved the substitution of two symmetrical acetyl groups in the 4-chloro-oxyphenisatin diacetate molecule with two isobutyl groups. Ultimately, the novel oxyphenisatin analogue was identified as (5-chloro-2-oxoindolin-3,3-ylidene) bis (4,1-phenylbutan-2-yl) diisobutyrate and designated as 4-Chloro-oxyphenisatin diisobutyrate. Finally, a quantitative analysis of the novel unknown compound in the jelly candy revealed a concentration of 6 mg per pellet. Based on the recommended daily consumption of one pellet, as indicated on the product packaging, the level of illegal additives may lead to diarrhoea and consequently poses a risk to human health. To the best of our knowledge, this represents the first report on the identification of 4-Chloro-oxyphenisatin diisobutyrate.
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MICROCHEMICAL JOURNAL
·
2024
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IF 4.9
Fuyang Gu,Ziting Ma,Yannuo Liu,Xiaoyan Wang,Peng Du,Yang Jiang,Guowei Xiao,Yanmei Qu,Changhai Xu
The blend of hydrogen peroxide (H 2 O 2 ) and N-[4-(triethylaminomethyl)benzoyl]caprolactam chloride (TBCC) boosts exceptional bleaching efficacy under low temperature and weak alkaline conditions, greatly reducing energy consumption. Nevertheless, the intricate perhydrolysis pathway of TBCC within a hydrogen peroxide solution and its subsequent impact on the whiteness of fabrics during this process have remained elusive. This study presents an integrated analytical methodology that harnesses ultraviolet spectrophotometry, fluorescent probes, confocal fluorescence microscopy, and liquid chromatography-mass spectrometry (LC-MS) to examine the pivotal aspects of the cotton fabric bleaching process. Specifically, we focus on the detection and quantification of the critical product N-4-(triethylammoniomethyl)peracetic acid (TPA) in the bleaching reaction. The research detected and quantified TPA, while emphasizing that insufficient concentrations and abbreviated durations of TPA significantly compromise the bleaching efficacy. Notably, variations in the TPA generation rate under different conditions revealed why TBCC exhibits exceptional bleaching performance under low-temperature and mildly alkaline conditions. This insight provides new perspectives and valuable insights into the critical factors influencing the bleaching process. The method is also applicable to other bleaching activators, and by controlling these factors in conjunction with actual conditions, it is possible to effectively reduce energy waste and provide valuable information for optimizing future practices in the field of bleaching.
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CELLULAR SIGNALLING
·
2024
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IF 4.4
Wentao Hu,Menghan Wang,Guifang Sun,Limin Zhang,Hong Lu
Parkinson's disease (PD) is a gradually debilitating neurodegenerative syndrome. Here, we analyzed GSE7621 chip data obtained from the Gene Expression Omnibus (GEO) database to explore the pathogenesis of PD. Early B Cell Factor 3 (EBF3), a member of the highly evolutionarily conserved EBF-transcription factor family, is involved in neuronal development. EBF3 expression is low in the substantia nigra of patients with PD. However, whether EBF3 is implicated in dopaminergic neuron death during PD has not yet been investigated. Therefore, we aimed to reveal the potential anti-apoptotic effect and molecular mechanism of EBF3 in PD. We established a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mouse model in vivo and a 1-methyl-4-phenylpyridine (MPP + )-induced SH-SY5Y cell model in vitro . EBF3 was downregulated in the substantia nigra of PD mice and SH-SY5Y cells treated with MPP + , and the m6A methylation modification level was low. Fat mass and obesity-associated protein (FTO) siRNA upregulated m6A methylation modification of EBF3 and extended the EBF3 mRNA half-life. Functionally, as demonstrated by the results of the open-field test, pole test and gait analysis, EBF3 overexpression ameliorated MPTP-induced behavioral disorder. Further, EBF3 overexpression suppressed neuronal apoptosis in vivo , as evidenced by decreased TUNEL + cells, and the increased activation of caspase-3 and caspase-9. Similar results were obtained in vitro , as reflected by increased cell viability, decreased LDH activity and restored mitochondrial function, collectively protecting SH-SY5Y cells from MPP + -induced apoptosis. Mechanistically, the results of luciferase reporter, ch-IP and DNA pull-down assays confirmed that, as a transcription factor, EBF3 bound to the promoter of CNTNAP4 (a protein associated with neuronal differentiation) and directly regulated CNTNAP4 transcription. Strikingly, CNTNAP4 knockdown markedly abolished the effect of EBF3 on cell apoptosis, thus aggravating PD. In conclusion, the low level of m6A methylation modification may contribute to the low expression of EBF3 during PD. Additionally, EBF3 attenuates PD by activating CNTNAP4 transcription, suggesting that EBF3 may be a novel therapeutic target in PD.
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CHEMICAL ENGINEERING JOURNAL
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2011
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IF 3.074
Xue Wang,Hanping Mao,Weihong Huang,Wei Guan,Xiaohua Zou,Jianming Pan,Yongsheng Yan
Microwave heating initiated suspension polymerization was developed to prepare 2-amino-4-nitrophenol (4-NAP) magnetic molecularly imprinted polymers (mag-MIPs) with magnetic wollastonite composites as support, resulting in much shorter polymerization time and better particle morphology. Mag-MIPs were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) analysis, transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), thermogravimetric analysis (TGA) and vibrating sample magnetometer (VSM). Static adsorptive experiment was applied to investigate the adsorption property and selectivity of the mag-MIPs. The results of the equilibrium rebinding experiments and the selective adsorption experiments showed that these imprinted polymers, not only display excellent affinity and selective ability to the 4-NAP, but also exhibited good superparamagnetic to an external magnetic field. Applied as a sorbent, the imprinted sorbent was used for the determination of 4-NAP in a mixture solution by high-performance liquid chromatography (HPLC).
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JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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2024
·
IF 2.3
Wei-Ming Chai,Qiuhan Bai,Qiuxia Pan,Linjun Wang,Du Zhu
6,7-Bis-(2-methoxyethoxy)-4(3 H )-quinazolinone (BMEQ) was selected from quinazolinones for its strong tyrosinase inhibitory activity ( IC 50 = 160 ± 6 μM). It suppressed tyrosinase activity in a competitive way and quenched the fluorescence of the enzyme through a static mechanism. The binding of BMEQ to tyrosinase increased the hydrophobicity of the latter and facilitated non-radiative energy transfer between them. The formation of BMEQ–tyrosinase complex was driven by hydrogen bonds and hydrophobic interactions, and it loosened the basic framework structure of tyrosinase, affecting the conformation of the enzyme, and leading to a decrease in tyrosinase activity. In addition, the BMEQ postponed the oxidation of phenolics and flavonoids by inhibiting polyphenol oxidase (PPO) and peroxidase (POD), which resulted in the inhibition of the browning of fresh-cut apples. This study identified a novel tyrosinase inhibitor BMEQ and verified its potential application for improving the preservation of postharvest fruits.
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MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2024
·
IF 2.5
Yang Hu,Xinming Pu,Jinggang Wang,Hongdong Zhang,Liping Yang
High performance copolyesters with ultrahigh glass transition temperature ( T g ) are crucial in emerging technology fields such as electronic devices, automotives, and coatings. In this paper, a series of poly(ethylene bis[4-(2-hydroxyethoxy) phenyl] sulfone 2,6-naphthalate) (PESNs) with high T g are synthesized by the copolymerization of 2,6-naphthalenedicarboxylic acid (2,6-NDA), bis[4-(2-hydroxyethoxy) phenyl] sulfone (BHEBS), and ethylene glycol (EG). The chemical structure and compositions of prepared copolymers are confirmed by hydrogen nuclear magnetic spectra ( 1 H NMR). T g of copolyesters (121.1–152.2 °C) depends on composition and is comparable to that of polyetheretherketone (PEEK: 143 °C), increasing as the amount of BHEBS units is increased. The Fox, Gordon–Taylor, and Kwei equations are employed to evaluate the variation of T g with respect to the composition of PESNs, respectively. In these three equations, the data calculated by Kwei equation fit the actual results best, attributed to the peculiar dielectric properties. In addition, PESNs show good solubility in 1,1,2,2-tetrachloroethane, DMF, and DMSO, which is conducive to meet the needs of polyester coating.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2024
·
IF 3.3
Kangyu Zhao,Xin Long,Junle Li,Ying Wang,Ping Lan,Yong Wang
BACKGROUND The Maillard reaction involves the interaction of various amino acids and reducing sugars, resulting in food browning. It often produces appealing aromas and flavors. The complexities of the reaction are such that it can be challenging to identify the often numerous and frequently volatile products formed by it. In the present study, we sought to identify and evaluate an unusual product with anti-oxidant activity arising from a fructose-histidine Maillard reaction model. The anti-oxidant profile of this product was assessed by computational means. RESULTS The fructose-histidine Maillard reaction products (FH-MRPs) were generated by heating a 2:1 mixture of the sugar and the amino acid at 140 °C for 2 h. Chromatographically separable fractions, labelled DM-1 to DM-8, were obtained using silica gel as the stationary phase and dichloromethane/methanol (DCM/MeOH) mixtures as the mobile one. Fraction DM-5 exhibited the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and further bio-assay guided fractionation led to isolation and identification of 1-(1 H -imidazo[4,5- c ]pyridin-4-yl)ethenone (IMPE) as the active principal, the structure of which was established by nuclear magnetic resonance (NMR) spectroscopic and mass spectral techniques. A mechanism for the formation of IMPE from its precursors is proposed. Density functional theory (DFT) calculations suggest this novel heterocyclic compound exerts its anti-oxidant effects by interacting with DPPH and 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals. Essentially, IMPE was non-toxic below 300 ug mL −1 , showing a concentration-dependent free radical clearance capacity and reducing power within the 100–1000 μg mL −1 range, and moreover, exhibiting significant Fe 2+ chelating abilities wihin the 50-200 μg mL −1 range. CONCLUSION This study identified the unique FH-MRP, IMPE, and found that it acts as food antioxidant through the chelation of metal ions. © 2024 Society of Chemical Industry.
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ACS Catalysis
·
2025
·
IF 13.1
Hongzhong Wang,Kai Chen,Junheng Huang,Chengchao He,Qinlong Zhang,Junwei Li,Zhifang Zhang,Zhenhai Wen
4-Nitrophenol (4-NP), a toxic and recalcitrant pollutant, necessitates effective remediation strategies. Here, a noble metal-free NiCu-NC electrocatalyst is developed, where dual atomic Ni–Cu sites are elegantly anchored on a rhombic dodecahedral nitrogen-doped carbon matrix, enabling selective electrochemical reduction of 4-NP into value-added p-aminophenol (4-AP). This catalyst achieves a low overpotential and a high Faradaic efficiency of 95.7% for 4-AP production. Density functional theory (DFT) calculations reveal that the NiCu dual-atom configuration minimizes the energy barrier for the rate-determining step, thereby enhancing catalytic performance. Furthermore, a hybrid acid/alkali flow electrolysis cell is designed to couple the cathodic 4-NP reduction with the anodic oxygen evolution. This system maintains stable operation for over 400 h while maintaining a high Faradaic efficiency (>91%) and near-complete conversion (99%) for valorization of 4-NP. This work demonstrates a practical electrocatalytic approach for pollutant transformation and chemical production with potential implications for wastewater treatment and resource utilization.
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JOURNAL OF CHROMATOGRAPHY A
·
2024
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IF 3.8
Jiana Lin,Xiaoyan Ouyang,Yuling Hu,Gongke Li,Qisheng Zhong
Acurate and sensitive determination of hazards from food contact materials is important to monitor food safety. It is necessary to excavate efficient adsorbent for simultaneous recognition and adsorption of food hazards of trace level for sample preparation. In this work, β-cyclodextrin and calix[4]arene were employed as hybrid functional monomers to prepare macrocyclic porous organic polymer (β-CD-CX4 POP). It was proved that the supramolecular cavities of β-CD-CX4 POP could form inclusion complexes with fluorescent whitening agents (FWAs) through host-guest recognition, which greatly improved the adsorption performance. The hydrophobic cavities of β-cyclodextrin and calix[4]arene of β-CD-CX4 POP exhibited synergistic effect for simultaneous recognition of FWAs. The high-throughput enrichment of FWAs was realized by β-CD-CX4 POP membranes coupled with a multiple-channel syringe pump. Based on membrane-based solid-phase extraction combined with UHPLC-MS/MS, a sensitive analytical method was established to determine six FWAs. The LODs was in range of 3-50 ng/L with the linear range of 0.02-100 μg/L. The developed method was used to quantify FWAs in bread wrapper and bread, and the spiked recoveries ranged from 78.1%-119% with RSD of 2.3%-9.7%. This work indicated that β-CD-CX4 POP was promising for the simultaneous recognition and adsorption of FWAs migrating from food contact materials.
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FASEB JOURNAL
·
2024
·Vol. 38
·期 13
·e23781
·
IF 4.4
Wenfang Lai,Siying Huang,Junjie Liu,Binbin Zhou,Zhengshuang Yu,John Brown,Guizhu Hong
Reactive astrocytes are important pathophysiologically and synthesize neurosteroids. We observed that LPS increased immunoreactive TLR4 and key steroidogenic enzymes in cortical astrocytes of rats and investigated whether corticosteroids are produced and mediate astrocytic TLR4-dependent innate immune responses. We found that LPS increased steroidogenic acute regulatory protein (StAR) and StAR-dependent aldosterone production in purified astrocytes. Both increases were blocked by the TLR4 antagonist TAK242. LPS also increased 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) and corticosterone production, and both were prevented by TAK242 and by siRNAs against 11β-HSD1, StAR, or aldosterone synthase (CYP11B2). Knockdown of 11β-HSD1, StAR, or CYP11B2 or blocking either mineralocorticoid receptors (MR) or glucocorticoid receptors (GR) prevented dephosphorylation of p-Ser 9 GSK-3β, activation of NF-κB, and the GSK-3β-dependent increases of C3, IL-1β, and TNF-α caused by LPS. Exogenous aldosterone mimicked the MR- and GSK-3β-dependent pro-inflammatory effects of LPS in astrocytes, but corticosterone did not. Supernatants from astrocytes treated with LPS reduced MAP2 and viability of cultured neurons except when astrocytic StAR or MR was inhibited. In adrenalectomized rats, intracerebroventricular injection of LPS increased astrocytic TLR4, StAR, CYP11B2, and 11β-HSD1, NF-κB, C3 and IL-1β, decreased astrocytic p-Ser 9 GSK-3β in the cortex and was neurotoxic, except when spironolactone was co-injected, consistent with the in vitro results. LPS also activated NF-κB in some NeuN + and CD11b + cells in the cortex, and these effects were prevented by spironolactone. We conclude that intracrine aldosterone may be involved in the TLR4-dependent innate immune responses of astrocytes and can trigger paracrine effects by activating astrocytic MR/GSK-3β/NF-κB signaling.
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Scientific Reports
·
2024
·Vol. 14
·期 1
·1-12
·
IF 3.8
Jiang Tianying,Wang Yanhui,Yu Zhiyu,Du Liangwei
Herbicides are widely used to control weeds in agriculture filed, however, the excessive use of the conventional formulation causes harmful side effects on the environment. To relieve this problem, natural polymer nanoparticles as herbicide carrier were rapidly developed and applied in recent years. In the present study, chitosan/tripolyphosphate (CS/TPP) nanoparticles were synthesized as nanocarrier to load herbicide 4-chloro-2-methylphenoxyacetate sodium salt (MCPA-Na). The encapsulation efficiency (EE) of 51.32% was obtained through measuring indirectly by high performance liquid chromatography (HPLC). The free and MCPA-Na-loaded CS/TPP nanoparticles were characterized by using dynamic light scattering (DLS), zeta potential, Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The encapsulation of MCPA-Na in CS/TPP nanoparticles resulted in the change of MCPA-Na release profile in different pH media and displayed effective sustained-release under neutral condition. The evaluation of herbicidal activity against Bidens pilosa L. showed that the efficacy enhancement of MCPA-Na was realized after encapsulation in CS/TPP nanoparticles. The proposed herbicide nanoformulation presented a good potential as a sustainable alternative for weed control in agriculture.
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