NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
·
2019
·Vol. 393
·期 1
·57-65
·
IF 2.058
Zhai Jiajia,Li Zeping,Zhang Huifeng,Ma Louyan,Ma Zhengquan,Zhang Yi,Zou Jian,Li Mo,Ma Li,Li Xiaomiao
The present study has been designed and carried out to evaluate the potential of coptisine on diabetic nephropathy. Diabetes was induced in SD rats through one single intraperitoneal injection of streptozotocin (65 mg/kg) method, and then diabetic rats were orally administered with 25 mg/kg/day coptisine or 50 mg/kg/day coptisine for 8 weeks. Severe impairment of renal function in rats with diabetes was observed as indicated by increased urine protein excretion, kidney hypertrophy index, serum creatinine level, and blood urea nitrogen level. Oxidative stress damage was observed as indicated by increased levels of reactive oxygen species, malondialdehyde, and decreased levels of glutathione, superoxide dismutase, and catalase. However, these alterations in kidneys of rats with diabetes were alleviated by administration of coptisine. Furthermore, the expression levels of nuclear factor-erythroid 2-related factor 2 (Nrf2) and its targeted antioxidative genes heme oxygenase 1 and NADPH quinone oxidoreductase 1 in the diabetic kidneys were significantly increased after coptisine treatment. These results suggested that coptisine ameliorated oxidative renal injury in diabetic rats, and the possible mechanisms for the renoprotective effects of coptisine may be related to activation of the Nrf2 signaling pathway.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 9
·1-9
·
IF 5.479
Chen Han,Wu Xingxing,Zhao Rui,Zheng Zhou,Yuan Qunhui,Dong Zhijun,Gan Wei
The integration of derivatives of granular metal-organic frameworks (MOFs) and an electrically conductive carbon substrate is an effective way to circumvent the deficiency of powdered pristine MOFs or MOF-derived carbon in practical application. The authors describe the use of graphite oxide (GO) as a substrate for in-situ assembly with the zeolitic imidazole framework ZIF-67. The GO and ZIF-67 composites were converted, via pyrolysis, into reduced graphite oxide loaded with Co/N-co-doped carbon polyhedrons (ZIF-67C@rGO). By using various amounts of GO, a series of ZIF-67C@rGO- x with different fractions of GO were synthesized and utilized as electrode modifiers for the detection of the antibiotic metronidazole (MNZ). The results revealed that the ZIF-67C@rGO-0.06 display best sensing performance. This is likely to be due to its hierarchically open pores, abundant active sites and good electrical conductivity. The sensor, best operated near a working potential around −0.6 V (vs. SCE), has a linear response in the 0.5 to 1000 μM MNZ concentration range and a 0.05 μM detection limit. The sensor was applied to the analysis of pharmaceutical samples where it showed excellent selectivity, good repeatability and satisfying recoveries.
Schematic representation of preparation and application of ZIF-67C@rGO- x .
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Applied Nanoscience
·
2019
·Vol. 10
·期 3
·775-786
·
IF 3.198
Jiang Jun,Hou Li,Lu Zhongyuan,Li Jun,Wang Jinxin,Yang Ying,Niu Yunhui
Nanosilica-modified swelled bentonite used as a pore-forming agent was introduced into cement pastes for the preparation of nanopore-rich lightweight cement pastes (NLCPs). The effect of nanosilica on the pore structure and hardened properties of NLCPs and their influence mechanisms were investigated. The results indicated that the introduction of nanosilica reduced pore size and improved nanopore content, due to increased platelets in voids, voids filled by hydration products and unreacted nanosilica. The percentage of nanopore content improved from 67.2 to 94.7% and 55.5–71.3% after, respectively, introducing a 60% and 80% bentonite slurry with increasing nanosilica replacement from 0 to 20%, and the median pore size decreased from 48.6 to 30.9 nm and 80.4–47.5 nm, respectively. The thermal insulation performance and compressive strength of NLCP were gradually enhanced by adding nanosilica from 0 to 20%. When nanosilica content was 20%, an increasing rate of 55.2% and 220.0% in 7-day compressive strength and 65.3% and 211.1% in 28-day compressive strength, with a 20.7% and 10.5% reduction in thermal conductivity at porosity of 61.3% and 72.3% can be achieved, respectively.
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JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
·
2019
·Vol. 24
·期 7
·1077-1085
·
IF 3.632
Wang Xiaoqing,Sun Qian,Zhao Liming,Gong Shuwen,Xu Li
Hydrogen polysulfides (H 2 S n , n > 1), as the oxidized forms of H 2 S, have attracted increasing attention these years due to their involvement in signaling transduction and cytoprotective processes. It is necessary to detect H 2 S n in living systems for the study of their functions. In this work, we report a BODIPY-based near-infrared emitting fluorescence probe NIR-PHS1 , with “turn-on” response, rapid response rate (within 10 min), outstanding selectivity and excellent sensitivity (detection limit = 12 nM) response towards H 2 S n . The probe was successfully applied to the visualizing of endogenous H 2 S n in living cells. Moreover, it can be used for near-infrared in vivo imaging of H 2 S n in living mice. Therefore, NIR-PHS1 could be a potential imaging tool to study the biological roles of H 2 S n in living systems.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 103
·期 19
·7983-7995
·
IF 3.67
Guo Jingui,Xie Yixiao,Yu Zhu,Meng Geng,Wu Zhe
For the first time, Lactobacillus plantarum strains carrying heterologous genes encoding multifunctional glycoside hydrolases were constructed and used as additives for alfalfa silage. The chemical characteristics, nonstructural carbohydrate composition, and fermentation quality of alfalfa silage were examined. The supernatant of L. plantarum expressing CbXyn10C and Bgxg1 (LP11AG) showed activities on xylan, Avicel, and carboxymethylcellulose (CMC), while the supernatant of the wild-type L. plantarum showed no activity. When LP11AG was used as silage additive, the water-soluble carbohydrate content of alfalfa silage increased by 72%, 55%, and 155% compared with control when the silage was stored at 20 °C, 30 °C, and 40 °C, respectively. With LP11AG being used as an additive for the alfalfa silage stored at 20 °C, the hemicellulose, cellulose, and acid detergent ligninin (ADL) contents decreased by 17%, 6%, and 14% compared with the control ( p < 0.05), respectively. Compared with the corresponding original contents, the contents of glucose, arabinose, galactose, and fructose detected in silage treated with LP11AG after 45 days of ensiling increased by 55%, 1494%, 68%, and 5% , respectively, when stored at 40 °C. Raffinose and stachyose, originally present in alfalfa, disappeared after ensiling. In conclusion, our results suggest that LP11AG provides a substantial benefit as a silage additive.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 15
·3587-3595
·
IF 3.307
Chen Guilin,Wu Jianlin,Li Na,Guo Mingquan
Rhamnus davurica Pall. ( R. davurica ) has been used as a traditional medicine for many years in China and abroad and shown a wide spectrum of biological activities. Previously, we reported the phytochemical fingerprinting profile of R. davurica , its distinct anti-proliferative activities against HT-29 and SGC-7901 cell lines, and the topoisomerase I (Top I) ligands based on bio-affinity ultrafiltration and HPLC-MS (UF-HPLC-MS). Nevertheless, among the 32 peaks detected in the fingerprinting profile, the common bioactive constituents responsible for the anti-inflammatory and anti-proliferative activities in the extracts remain elusive. To further explore the specific responsible components for their diversified activities and their potential action targets/mechanisms, the method based on bio-affinity UF-HPLC-MS using therapeutic targets like Top I and cyclooxygenase 2 (COX-2) was established to rapidly screen and identify the ligands binding to these known target enzymes. As a result, 12 components were revealed as potential Top I ligands along with 11 components as potential COX-2 ligands, where several components were revealed to possess both activities. Further validations of these bioactive components have also been conducted and confirmed their highlighted activities. This integrated method of UF-HPLC-MS exhibits high efficiency in rapidly screening for multi-target bioactive components responsible for multiple pharmacological effects from the complex natural products and could be very useful to explain the complex action mechanisms of herb medicines in a complex multi-component and multi-target mode at the molecular level.
Schematic diagram of UF-HPLC-MS assay to screen for Top I and COX-2 ligands. The principle of the assay usually involves the following steps: incubation, ultrafiltration, and identification.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 3
·1-10
·
IF 5.705
Li Hongji,Wang Xiaonan,Wang Zirun,Wang Yan,Dai Jiangdong,Gao Lin,Wei Maobin,Yan Yongsheng,Li Chunxiang
A new method is described for the determination of the pesticide λ-cyhalothrin (LC). It combines SERS detection with molecular imprinting and largely improves selectivity. A multilayer surface imprinted nanocomposite was synthesized in two steps on a nanostructure of type SiO 2 @rGO@Ag acting as a substrates. Firstly, the surface of the SiO 2 @rGO@Ag composite was modified with self-polymerized dopamine. Secondly, surface-initiated polymerization was carried out to prepare a molecularly imprinted polymer (MIP) using LC as the template. The use of this SiO 2 @rGO@Ag-MIP allows for excellent SERS based detection and has high selectivity for LC. The Raman intensity and LC concentration present perfect linear relationship between 10 −5 to 10 −9 mol L −1 and the detection limit is 3.8×10 −10 mol L −1 . All the procedures are conducted in aqueous or ethanol solution.
Schematic of a new method for determination of the pesticide λ-cyhalothrin. It combines SERS detection with molecular imprinting and largely improves selectivity. A multilayer surface imprinted nanocomposite was synthesized in two steps on a nanostructure of type SiO 2 @rGO@Ag acting as a substrates.
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Scientific Reports
·
2018
·Vol. 8
·期 1
·1-11
·
IF 4.122
Jiang Zhiqiang,Dong Xiaoyan,Yan Xin,Liu Yang,Zhang Lin,Sun Yan
Inhibition of amyloid β-protein (Aβ) aggregation is considered as a promising strategy for the prevention and treatment of Alzheimer’s disease. Epigallocatechin-3-gallate (EGCG) and curcumin have been recognized as effective inhibitors of Aβ aggregation. Herein, we proposed dual-inhibitor modification of hyaluronic acid (HA) to explore the synergistic effect of the two inhibitors. EGCG-modified HA (EHA) formed dispersed hydrogel structures, while EGCG-curcumin bi-modified HA (CEHA) self-assembled into nanogels like curcumin-modified HA (CHA). Thioflavin T fluorescent assays revealed that the inhibitory effect of CEHA was 69% and 55% higher than EHA and CHA, respectively, and cytotoxicity assays showed that the viability of SH-SY5Y cells incubated with Aβ and CEHA was 28% higher than that with Aβ and the mixture of EHA and CHA. These results clearly indicate the synergism of the two inhibitors. It is considered that the difference in the hydrophobicities of the two inhibitors made the bi-modification of HA provide a favorable CEHA nanostructure that coordinated different inhibition effects of the two inhibitors. This research indicates that fabrication of dual-inhibitor nanosystem is promising for the development of potent agents against Aβ aggregation and cytotoxicity.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2018
·Vol. 25
·期 12
·11970-11980
·
IF 2.8
Yang Zhihui,Liang Lifen,Yang Weichun,Shi Wei,Tong Yunping,Chai Liyuan,Gao Shikang,Liao Qi
Self-aggregation of bulk nano-hydroxyapatites (n-HAPs) undermines their immobilization efficiencies of heavy metals in the contaminated soils. Here, the low-cost, easily obtained, and environment-friendly filamentous fungi have been introduced for the bio-matrices of the hybrid bio-nanocomposites to potentially solve such problem of n-HAPs. According to SEM, TEM, XRD, and FT-IR analyses, n-HAPs were successfully coated onto the fungal hyphae and their self-aggregation was improved. The immobilization efficiencies of diethylene-triamine-pentaacetic acid (DTPA)-extractable Cd and Pb in the contaminated soils by the bio-nanocomposites were individually one to four times of that by n-HAPs or the fungal hyphae. Moreover, the Aspergillus niger- based bio-nanocomposite (ANHP) was superior to the Penicillium Chrysogenum F1-based bio-nanocomposite (PCHP) in immobilization of Cd and Pb in the contaminated soils. In addition, the results of XRD showed that one of the potential mechanisms of metal immobilization by the hybrid bio-nanocomposites was dissolution of n-HAPs followed by precipitation of new metal phosphate minerals. Our results suggest that the hybrid bio-nanocomposite (ANHP) can be recognized as a promising soil amendment candidate for effective remediation on the soils simultaneously contaminated by Cd and Pb.
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COLLOID AND POLYMER SCIENCE
·
2018
·Vol. 296
·期 3
·495-502
·
IF 1.967
Zhang Rongli,Xu Xiaofang,Fan Xiaoxia,Yang Renchun,Wu Tao,Zhang Cuige
A novel kind of biocompatible conducting micelles self-assembled from commercial poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) and chitosan (CS) via electrostatic interaction. The resultant micelles were then used to provide a platform for fabricating high sensitive biosensor. It is expected that the introduction of chitosan in micelles can solve the problem of PEDOT:PSS film easily peeling off from the surface of the electrode. The micelles with nanostructure on the electrode can facilitate the immobilization of enzyme due to the large surface to volume ratio. The good conductivity of the commercial PEDOT:PSS can provide a platform to accelerate the electron transfer and facilitate the signal transduction from the micelles film to the underlying electrode. These advantages endowed the biosensor with high sensitivity for H 2 O 2 detection. Under the optimal conditions, a linear ranged from 1 × 10 −10 to 1 × 10 −5 mol·L −1 with a detection limit of 3 × 10 −11 mol·L −1 were obtained for H 2 O 2 detection. The biosensor can be used to analyze H 2 O 2 in commercial disinfector with satisfactory results.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 7
·2011-2018
·
IF 3.307
Yu Lingling,Wen Chao,Li Xing,Fang Shiqi,Yang Lichuan,Wang Tony,Hu Kaifeng
Quantification of endogenous and exogenous plasma glucose can help more comprehensively evaluate the glucose metabolic status. A ratio-based approach using isotope dilution liquid chromatography tandem mass spectrometry (ID LC-MS/MS) with indirect multiple reaction monitoring (MRM) of the derivative tag was developed to simultaneously quantify endo-/exogenous plasma glucose. Using diluted D-[ 13 C 6 ] glucose as tracer of exogenous glucose, 12 C 6 / 13 C 6 glucoses were first derivatized and then data were acquired in MRM mode. The metabolism of exogenous glucose can be tracked and the concentration ratio of endo/exo-genous glucose can be measured by calculating the endo-/exo-genous glucose concentrations from peak area ratio of specific daughter ions. Joint application of selective derivatization and MRM analysis not only improves the sensitivity but also minimizes the interference from the background of plasma, which warrants the accuracy and reproducibility. Good agreement between the theoretical and calculated concentration ratios was obtained with a linear correlation coefficient ( R ) of 0.9969 in the range of D-glucose from 0.5 to 20.0 mM, which covers the healthy and diabetic physiological scenarios. Satisfactory reproducibility was obtained by evaluation of the intra- and inter-day precisions with relative standard deviations (RSDs) less than 5.16%, and relative recoveries of 85.96 to 95.92% were obtained at low, medium, and high concentration, respectively. The method was successfully applied to simultaneous determination of the endo-/exogenous glucose concentration in plasma of non-diabetic and type II diabetic cynomolgus monkeys.
Graphical Abstract The scheme of the proposed ratio-based approach using isotope dilution LC-MS/MS with indirect MRM of the derivative tag for simultaneous quantification of endogenous and exogenous plasma glucose.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2018
·Vol. 29
·期 22
·19111-19122
·
IF 2.324
Duan Haojie,He Yue,Li Shouchun,Liu Li,Xu Suyang,Li Yu,Li Haiying,Gong Yimin,Liang Qingcheng,Cheng Yali
The pure and Tb-doped In 2 O 3 beaded-porous nanotubes (BPNTs) are fabricated by simple electrospinning method. The crystal phase, morphology and chemical composition of the obtained samples were analyzed by characteristic techniques (XRD, FE-SEM, TEM, EDS, XPS, etc.). The mechanism of forming BPNTs structure was studied, and phase separation plays a key role in the process of formation. A series of sensing tests toward formaldehyde gas showed that 6 mol% Tb-doped In 2 O 3 BPNTs exhibited the best sensing performance: high response (75–50 ppm), shorter response and recovery times (2 s and 12 s), low limit of detection (LOD, 1.27–100 ppb), good selectivity and long-term stability, etc. The significantly improved gas sensing properties were mainly resulted from their high surface basicity, enriched defects (oxygen vacancies), small crystallite size and complex porous structures.
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