Journal of Environmental Chemical Engineering
·
2022
·
IF 7.968
Hongyang Pan,Jiamin Gu,Kaiyang Hou,Junyao Li,Yu Wang,Yiying Yue
Photocatalytic technology has received considerable attention due to its advantages of high efficiency, economy and thorough degradation of pollutants, making it the most promising sewage treatment technology. However, the difficulty in recycling usually limits its applications. In our research, we developed a composite aerogel with excellent mechanical properties, photocatalytic properties and recyclability to adsorb and degrade rhodamine B (RhB) dye. In this composite, g-C 3 N 4 /BiOBr was loaded on the matrix of rGO and chitosan (CS), with borax as the crosslinking agent. The adsorption experiment showed that the equilibrium adsorption capacity of g-C 3 N 4 /BiOBr@CS/rGA aerogels were 3.08 mg/g. The composite photocatalyst of g-C 3 N 4 /BiOBr could effectively inhibit the recombination of photo-generated carriers, loaded on the rGO matrix with admirable conductivity, which further improved the photocatalytic performance. The degradation rate of RhB could reach to 94.6% at 180 min under the neutral condition of pH= 7. In addition, the amine group of CS reacted with the carboxyl group of GO to form an amide group, it enhanced the mechanical properties of the aerogels. The compressive strength was 0.152 MPa at 50% strain, and there was basically no decrease after 5 cycles. The composite aerogels exhibited good recycling performance, and the degradation rate was about 80% after 4 cycles.
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高影响因子
NEUROSCIENCE
·
2024
·
IF 2.9
Xuejing Hou,Shanshan Xiao,Xiaohong Xu,Mingze Qin,Xuebing Cheng,Xiangping Xu
Epilepsy is a progressive neurodegenerative disease highlighted by recurrent seizures, neuroinflammation, and the loss of neurons. Microglial dysfunction is commonly found in epileptic foci and contributes to neuroinflammation in the initiation and progression of epilepsy. Glycoprotein non-metastatic melanoma protein B (GPNMB), a transmembrane glycoprotein, has been involved in the microglial activation and neuroinflammation response. The present study investigated the functional significance of GPNMB in epilepsy. A proven model of epilepsy was established by intraperitoneal injection of pilocarpine to male Sprague Dawley rats. Lentivirus vectors carrying GPNMB or GPNMB short hairpin RNA (shGPNMB) were injected into the hippocampus to induce overexpression or knockdown of GPNMB. GPNMB expression was significantly upregulated and overexpression of GPNMB in the hippocampus reduced seizure activity and neuronal loss after status epilepticus (SE). We here focused on the effects of GPNMB deficiency on neuronal injury and microglia polarization 28 days after SE. GPNMB knockdown accelerated neuronal damage in the hippocampus, evidenced by increased neuron loss and neuronal cell apoptosis. Following GPNMB knockdown, M1 polarization (iNOS) and secretion of pro-inflammatory cytokines IL-6, IL-1β, and TNF-α were increased, and M2 polarization (Arg1) and secretion of anti-inflammatory cytokines IL-4, IL-10, and TGF-β were decreased. BV2 cells were used to further confirm the regulatory role of GPNMB in modulating phenotypic transformations and inflammatory cytokine expressions in microglia. In conclusion, these results indicated that GPNMB suppressed epilepsy through repression of hippocampal neuroinflammation, suggesting that GPNMB might be considered the potential neurotherapeutic target for epilepsy management and play a protective role against epilepsy by modulating the polarization of microglia.
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高影响因子
PLoS One
·
2014
·Vol. 9
·期 4
·e94418
·
IF 3.534
Wu Li,Quanju Zhao,Wanyan Deng,Tian Chen,Minqiang Liu,Jianping Xie
Intracellular survival plays a central role in the pathogenesis of Mycobacterium tuberculosis , a process which depends on an array of virulence factors to colonize and replicate within the host. The M. tuberculosis iron regulated open reading frame (ORF) rv3402c , encoding a conserved hypothetical protein, was shown to be up-regulated upon infection in both human and mice macrophages. To explore the function of this ORF, we heterologously expressed the rv3402c gene in the non-pathogenic fast-growing Mycobacterium smegmatis strain, and demonstrated that Rv3402c, a cell envelope-associated protein, was able to enhance the intracellular survival of recombinant M. smegmatis . Enhanced growth was not found to be the result of an increased resistance to intracellular stresses, as growth of the Rv3402c expressing strain was unaffected by iron depletion, H 2 O 2 exposure, or acidic conditions. Colonization of macrophages by M. smegmatis expressing Rv3402c was associated with substantial cell death and significantly greater amount of TNF-α and IL-1β compared with controls. Rv3402c-induced TNF-α and IL-1β production was found to be mediated by NF-κB, ERK and p38 pathway in macrophages. In summary, our study suggests that Rv3402c delivered in a live M. smegmatis vehicle can modify the cytokines profile of macrophage, promote host cell death and enhance the persistence of mycobacterium within host cells.
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高影响因子
ONCOLOGY REPORTS
·
2024
·Vol. 52
·期 4
·1-8
·
IF 3.8
Tong Wang,Xu Ye,Hao Jiang,Yu Gao
B‑cell lymphoma is difficult to cure because of its biological and clinical heterogeneity, and due to native chemoresistance. Immunotherapies that overcome cancer‑induced immune evasion have been the center of recent developments in oncology. This is emphasized by the accomplishment of various agents that disrupt programmed cell death protein 1 (PD‑1)‑mediated immune suppression in diverse tumors. However, while PD‑1 blockade has been effective in numerous malignancies, a significant proportion of cancers, including B‑cell lymphoma, show certain rates of primary resistance to these therapeutic strategies. Histone deacetylase inhibitors (HDACis) have exhibited anticancer activity though suppressing cell proliferation, inducing differentiation and triggering apoptosis. The present study aimed to explore a therapeutic strategy combining a HDACi (romidepsin) and PD‑1 blockade (BMS‑1) in B‑cell lymphoma, utilizing a constructed mouse model of B‑cell lymphoma. The IC50 of the two inhibitors was confirmed by MTT assay, and their inhibitory effects were revealed to be dose‑ and time‑dependent. The data demonstrated that the combined treatment of romidepsin and BMS‑1 synergistically inhibited the growth of B‑cell lymphoma. Furthermore, it was revealed that romidepsin and BMS‑1 synergistically triggered apoptosis in mouse B‑cell lymphoma. The synergistic effect of these agents was capable of activating tumor‑infiltrating lymphocytes, particularly CD3+CD4+ and CD3+CD8+ T cells. The results of the present study underscore the potential of HDAC inhibition in conjunction with PD‑1 blockade as a novel therapeutic approach for B‑cell lymphoma, highlighting the synergistic effects of these two mechanisms in enhancing antitumor immunity.
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高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 10
·100548
·
IF 3.721
Yingying Zhang,Liming Xu,Jingkun Xu,Weiqiang Zhou,Hui Zhang
Poly(3,4-ethylenedioxythiophene) and its derivatives provide an excellent platform as electrode materials for supercapacitors due to their superior stability and conductivity. In this study, a poly(2,3-dihydrothieno[3,4-b][1,4]dioxin-2-yl)methanol (PEDOT-MeOH) porous nanonet (PEDOT-MeOH-PNN), PEDOT-MeOH hollow nanotube array (PEDOT-MeOH-HNA) and PEDOT-MeOH-PNN coated PEDOT-MeOH-HNA (PEDOT-MeOH-PNN@PEDOT-MeOH-HNA) are successfully fabricated using a template-free electrodeposition of (2,3-dihydrothieno[3,4-b][1,4]dioxin-2-yl)methanol monomer. These three nanostructures were studied by Fourier transform infrared spectrum and Raman spectrum, scanning electron microscopy, thermal analysis and electrochemical methods, respectively. PEDOT-MeOH-PNN@PEDOT-MeOH-HNA has a specific capacitance of 40.5 mF cm−2 at 40 mV s−1, which is higher than PEDOT-MeOH-PNN (21.6 mF cm−2) and PEDOT-MeOH-HNA (35.5 mF cm−2). Moreover, the PEDOT-MeOH-PNN@PEDOT-MeOH-HNA based symmetric supercapacitor delivers excellent rate performance and superior cycling stability (90% of the initial capacitance remains after 10,000 cycles). The results indicate that the structural design of a supercapacitor electrode can improve their electrochemical performance, and promote the application and development of conducting polymers in the field of supercapacitors.
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高影响因子
CHEMICAL PHYSICS
·
2020
·
IF 1.771
Lizhi Zhao,Yaxiang Zhao,Rishun Li,Dihao Wu,Xingyu Xie,Hui Ye,Yuzhong Zhang,Qingping Xin
Metal-organic frameworks (MOFs) of copper-tetrakis-(4-carboxyphenyl)-porphyrin (CuTCPP) were prepared by a solvothermal route. The two-dimensional (2D) ultrathin nanosheets with larger specific surface area show higher adsorption capacity for rhodamine B (RhB), compared with the bulk MOF of CuTCPP. The binding mechanism of the 2D nanosheets with RhB was investigated based on spectroscopic methods . The results reveal that the fluorescence of RhB is efficiently quenched by 2D CuTCPP nanosheets , and the quenching mechanism is totally the static quenching by forming a ground state RhB-CuTCPP nanosheets complex with an apparent association constant of 3.953 × 10 3 M −1 . The interaction between RhB and CuTCPP nanosheets leads to the H-aggregate of RhB in the complex. Higher-order aggregate may also be involved in the complexation. Thermodynamic studies demonstrate that RhB-CuTCPP nanosheets interaction is a spontaneous process and driven by electrostatic and hydrophobic forces . The 2D CuTCPP nanosheets can be regenerated from the complex and used repeatedly.
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Polymers
·
2025
·Vol. 17
·期 8
·1030
·
IF 4.9
Chaoqun Wang,Tong Wu,Yidi Li,Jie Liu,Yanshai Wang,Kefeng Wang,Yang Li,Xuefei Leng
Amphiphilic block copolymers with complex topologies (e.g., star and brush topologies) have attracted significant attention in drug delivery owing to their superior performance over linear micelles. However, their precise synthesis and structure–property relationships require further investigation. In this study, hydroxylated polybutadiene with adjustable topology and hydroxyl group density was employed as a macroinitiator to synthesize well-defined amphiphilic poly (ethylene oxide)- b -poly( ε -caprolactone- ran - δ -valerolactone) (PEO- b -P(CL- ran -VL)) copolymers via ring-opening polymerization (ROP). A series of linear, star, linear–comb, and star–comb copolymers were prepared as curcumin-loaded micellar carriers for the study. The self-assembly behavior, drug encapsulation efficiency, and in vitro release profiles of these copolymers in aqueous environments were systematically investigated. The results demonstrated that increasing the branch length of star–comb copolymers effectively reduced micelle size from 143 to 96 nm and enhanced drug encapsulation efficiency from 27.3% to 39.8%. Notably, the star–comb architecture exhibited 1.2-fold higher curcumin encapsulation efficiency than the linear counterparts. Furthermore, the optimized star–comb nanoparticles displayed sustained release kinetics (73.38% release over 15 days), outperforming conventional linear micelles. This study establishes a quantitative structure–property relationship between copolymer topology and drug delivery performance, providing a molecular design platform for programmable nanocarriers tailored to diverse therapeutic requirements of various diseases.
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2025
·Vol. 13
·期 38
·32562-32576
·
IF 9.5
Chenshuo Yuan,Dongwang Feng,Hui Ye,Pengkai Shan,Lin Ge,Han Chen,Yifeng Zheng,Sheng Cui
Reversible solid oxide cells (RSOCs) represent a promising technology for efficient energy conversion and storage. However, the performance is often limited by the sluggish oxygen kinetics and poor structural stability of the air electrode. The cobalt-free Bi0.5Sr0.5FeO3−δ (BSF) air electrode exhibits a comparatively desirable performance and is expected to be further optimized. Herein, we propose a B-site co-doping strategy by incorporating high valence Nb5+ and Ta5+ into BSF to synergistically optimize the oxygen transport capacity and surface reactivity. The optimized Bi0.5Sr0.5Fe0.8Nb0.1Ta0.1O3−δ (BSFN0.1T0.1) demonstrates exceptional oxygen ion diffusivity (Dchem = 4.378 × 10−4 cm2 s−1) and exchange kinetics (Kchem = 1.330 × 10−3 cm s−1), leading to a 69% reduction in polarization resistance (from 0.195 to 0.06 Ω cm2) at 750 °C. Notably, the BSFN0.1T0.1 air electrode maintains stable performance under 3–10% CO2 atmosphere, demonstrating superior CO2 tolerance. In fuel cell mode, the single cell delivers a peak power density of 616 mW cm−2 (98% enhancement over BSF) at 750 °C. In electrolysis cell mode, a current density of 1370 mA cm−2 is obtained at 750 °C and 1.5 V in 70% CO2/30% CO atmosphere (116% enhancement over BSF). The synergistic effect of Nb5+ and Ta5+ co-doping arises from their similar ionic radii, stable high valence states, and electronegativity differences, which stabilize the perovskite lattice and facilitate oxygen migration, thereby optimizing oxygen reduction/evolution reaction (ORR/OER) activity and electrochemical performance. This work provides a rational design strategy for advanced RSOC air electrodes.
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高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Bricard Mbituyimana,Longjun Luo,Fuyu Qi,Yuchi Jiang,Omar Mohammad Atta,Mohamed S. Kishta,Mostafa Maboruk,Min Wu,Wei Ge,Zhijun Shi,Guang Yang
Hypertrophic scar (HS) represents a natural consequence of abnormal fibroproliferation occurring during wound healing, which not only leads to tissue dysfunction but also causes psychological issues for patients. Up to now, no completely effective therapy for HS has been found. Salvianolic acid B (Sal B), one of the natural plant-derived compounds, has the capacity to suppress the fibrosis process in scarring treatment. Nevertheless, due to its poor skin-penetration ability and unsatisfactory stability in existing formulations, its current clinical applicability is restricted. In this study, inspired by the separation behavior of the thorns on the trunks of the silk floss tree, we developed a hyaluronic acid (HA) dissolving microneedle (MN) platform loaded with Sal B. This platform can enhance the sustainable delivery of Sal-B directly into scar tissue in a minimally invasive manner, thereby enhancing the efficacy of HS therapy and improving the stability of Sal B. To evaluate the therapeutic efficacy of Sal-B MN, we established a rabbit ear HS model. After being administered five times, Sal-B MN significantly reduced the scar elevation index (SEI) and down-regulated the expressions of collagen I, transforming growth factor beta 1 (TGF-β1), Signal Transducer and Activator of Transcription 3 (STAT3), and alpha-smooth muscle actin (α-SMA). Notably, these effects were still evident even after six months of storage at room temperature. This indicates that tree-inspired Sal-B MN holds great potential as a highly effective tool for HS treatment with a long shelf-life. This makes the system suitable for clinical application and storage under various conditions.
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PubMed
高影响因子
Molecular Medicine Reports
·
2024
·Vol. 30
·期 4
·1-10
·
IF 3.4
Ying Chen,Yu Li,Xiaojuan Li,Qingqing Fang,Feng Li,Shiyao Chen,Weichang Chen
Indole‑3‑propionic acid (IPA), a product of Clostridium sporogenes metabolism, has been shown to improve intestinal barrier function. In the present study, in vitro experiments using NCM460 human colonic epithelial cells were performed to investigate how IPA alleviates lipopolysaccharide (LPS)‑induced intestinal epithelial cell injury, with the aim of improving intestinal barrier function. In addition, the underlying mechanism was explored. NCM460 cell viability and apoptosis were measured using the Cell Counting Kit‑8 assay and flow cytometry, respectively. The integrity of the intestinal epithelial barrier was evaluated by measuring transepithelial electrical resistance (TEER). The underlying molecular mechanism was explored using western blotting, immunofluorescence staining, a dual luciferase reporter gene assay and quantitative PCR. The results showed that 10 µg/ml LPS induced the most prominent decrease in cell viability after 24 h of treatment. By contrast, IPA effectively inhibited LPS‑induced apoptosis in the intestinal epithelial cells. Additionally, >0.5 mM IPA improved intestinal barrier function by increasing TEER and upregulating the expression of tight junction proteins (zonula occludens‑1, claudin‑1 and occludin). Furthermore, IPA inhibited the release of pro‑inflammatory cytokines (IL‑1β, IL‑6 and TNF‑α) in a dose‑dependent manner and this was achieved via regulation of the Toll‑like receptor 4 (TLR4)/myeloid differentiation factor 88/NF‑κB and TLR4/TRIF/NF‑κB pathways. In conclusion, IPA may alleviate LPS‑induced inflammatory injury in human colonic epithelial cells. Taken together, these results suggest that IPA may be a potential therapeutic approach for the management of diseases characterized by LPS‑induced intestinal epithelial cell injury and intestinal barrier dysfunction.
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高影响因子
Small
·
2024
·
IF 13
Guofeng Zhong,Pengkun Lei,Peng Guo,Qin Yang,Yun Duan,Junbo Zhang,Mengyu Qiu,Kaijun Gou,Chen Zhang,Yan Qu,Rui Zeng
Wounds in harsh environments can face long-term inflammation and persistent infection, which can slow healing. Wound spray is a product that can be rapidly applied to large and irregularly dynamic wounds, and can quickly form a protective film in situ to inhibit external environmental infection. In this study, a biodegradable A and B combined multi-functional spray hydrogel is developed with methacrylate-modified chitosan (CSMA 1st ) and ferulic acid (FA) as type A raw materials and oxidized Bletilla striata polysaccharide (OBSP) as type B raw materials. The precursor CSMA 1st -FA/OBSP (CSOB-FA 1st ) hydrogel is formed by the self-cross-linking of dynamic Schiff base bonds, the CSMA-FA/OBSP (CSOB-FA) hydrogel is formed quickly after UV–vis light, so that the hydrogel fits with the wound. Rapid spraying and curing provide sufficient flexibility and rapidity for wounds and the hydrogel has good injectability, adhesive, and mechanical strength. In rats and miniature pigs, the A and B combined spray hydrogel can shrink wounds and promote healing of infected wounds, and promote the enrichment of fibrocyte populations. Therefore, the multifunctional spray hydrogel combined with A and B can protect irregular dynamic wounds, prevent wound infection and secondary injury, and be used for safe and effective wound treatment, which has a good prospect for development.
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高影响因子
MEDICAL SCIENCE MONITOR
·
2018
·
IF 1.894
Xuechan Tang,Xiaoli Xie,Xin Wang,Yan Wang,Xiaoyu Jiang,Huiqing Jiang
Background Piwi-interacting RNA (piRNA) is the largest class of small non-coding RNA, which has also been identified in somatic tissues, and aberrant expression of piRNAs in tumor tissues may be implicated in carcinogenesis. piR-823 is increased in liver cirrhosis and hepatocellular carcinoma (HCC). However, there is no report on the function of piR-823 in hepatic stellate cells (HSCs) activation during hepatic fibrosis. The present study investigated the role of piR-823 in HSC activation. Matreial/Methods Liver fibrosis was induced in mice by carbon tetrachloride (CCL 4 ) injection and bile duct ligation (BDL). The primary HSCs were isolated from mice and cultured. The expression of piR-823 was measured by real-time PCR. The effect of piR-823 on HSCs was evaluated by either sense sequence or antisense sequence of piR-823 carried by liposome. Proteins binding to piR-823 were assayed by RNA pull-down technique and liquid chromatography-mass spectrometry (LC-MS). Results Our data for the first time show that piR-823 is significantly upregulated in activated HSCs. Overexpression of piR-823 promoted HSC proliferation, α-SMA and COL1a1 production, whereas inhibition of piR-823 suppressed the activity of HSCs. Interestingly, the combination of piR-823 and EIF3B promoted TGF-β1 expression. Conclusions Our data illustrate a novel mechanism of piR-823 in HSC activities. The combination of piR-823 and EIF3B increased TGF-β1 expression, which activates HSCs in liver fibrosis. piR-823 may be a new target in the treatment of liver fibrosis.
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高影响因子
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