INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2017
·
IF 3.671
Yurui Xu,Sajid Asghar,Liu Yang,Zhipeng Chen,Hongying Li,Wenwen Shi,Yibo Li,Qinqing Shi,Qineng Ping,Yanyu Xiao
Nanoparticles based on chitosan hydrochloride (CSH)-hyaluronic acid (HA)-PEG were prepared for delivering curcumin (CUR) (CUR-PNPs) to brain tumor. CUR-PNPs of 245.9 nm and spherical morphology were obtained at optimized CSH/HA/PEG20000/CUR ratios with negative charge of about −27.2 mV and EE of approximately 93.3%. Cytotoxicity studies showed that CUR-PNPs improved drug’s anticancer activity in rat glioma cells (C6). The cellular uptake mechanism study showed active targeting of CUR-PNPs into C6 cells by HA mediated endocytosis . Clathrin-coated pit mediated endocytosis, clathrin-mediated endocytosis and macropincytosis were also identified as the entry pathways of PNPs into C6 cells. Pharmacokinetics of preparations in rats after i.v. administration further proved the superiority of CUR-PNPs (3.98 times greater than the area under the curve of CUR solution). In conclusion, CUR-PNPs might be a promising carrier for the therapy of brain tumors.
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Scientific Reports
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2017
·Vol. 7
·期 1
·1-8
·
IF 4.259
Wang Min,Zhang Lele,Zhang Guilong,Pang Tao,Zhang Xin,Cai Dongqing,Wu Zhengyan
This study reported an immediate approach for the degradation of three antibiotic (amoxicillin, ofloxacin, and cefradine) residues in medical intravenous infusion bottles (MIIBs) using high energy electron beam (HEEB) irradiation. The effects of irradiation doses, initial concentrations, initial pH, and scavengers of active radicals on the degradation of three antibiotic residues (ARs) were investigated, and the results displayed that 97.02%, 97.61% and 96.87% of amoxicillin, ofloxacin, and cefradine residues could be degraded in situ through HEEB irradiation respectively. Fourier transform infrared spectroscopy (FTIR) and high performance liquid chromatography-mass spectrometry (HPLC-MS) analysis demonstrated that ARs were mainly decomposed into inorganic ions and alkanes. Typically, the detailed degradation mechanism of ARs was also investigated, and the dominant active particle inducing the degradation of antibiotics during the HEEB irradiation process was demonstrated to be hydroxyl radical.
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ELECTROCHIMICA ACTA
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2017
·
IF 4.798
Yuanyuan Zhang,Fenpei Li,Xiangqing Liu,Jiaxing Lu,Guirong Zhang
Direct methanol fuel cell (DMFC) have the advantages of low emissions of carbon dioxide , high power density and high energy density, so they have a great potential to replace fossil fuels. It is well known that Pt with high activity is an ideal catalyst for methanol oxidation in an acidic medium. However, it is an expensive material, and cost of the DMFC containing Pt is prohibitively high and it becomes poisoned by CO ads which is formed during the methanol oxidation. In this work, for the first time, the preparation of activated carbon(YBC) doped carbon paste electrode (YBCPE) and carbon paste electrode (CPE) were carried out for comparison. The electrochemical properties of YBCPE and CPE were investigated by electrochemical impedance spectroscopy . The electrodes of Pt/YBCPE and Pt/CPE were prepared by electrodeposition of platinum on YBCPE and CPE, respectively, at a constant potential (−0.1 V vs. Ag|AgCl(sat)) in 0.5 M H 2 SO 4 containing 3 mM H 2 PtCl 6 . The behavior of electrodeposition of platinum on YBCPE and CPE was studied through the application of theoretical models, and the morphology of Pt/YBCPE and Pt/CPE were characterized by SEM. The electrochemical activities of these catalysts towards methanol electrooxidation were examined by cyclic voltammetry . The influence of YBC mass percentage in YBCPE on both the electrode activity and long-term stability of electrodes in methanol electrooxidation was studied. It was shown that the addition of activated carbon improved the electrocatalytic activity of Pt catalysts on corresponding YBCPE toward methanol electrooxidation.
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DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
·
2016
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IF 2.429
Jing Lu,Shuling Kan,Yi Zhao,Wenli Zhang,Jianping Liu
The purpose of this study was to develop the novel naproxen/esomeprazole magnesium compound pellets (novel-NAP/EMZ) depending on EMZ acid-independent mechanism which has been proved to be predominate in the mechanism of co-therapy with nonsteroidal anti-inflammatory drug. The novel-NAP/EMZ compound pellets, composed of NAP colon-specific pellets (NAP-CSPs) and EMZ modified-release pellets (EMZ-MRPs), were prepared by fluid-bed coating technology with desired in vitro release profiles. The resulting pellets were filled into hard gelatin capsules for in vivo evaluation in rats and compared with the reference compound pellets, consisted of NAP enteric-coated pellets (NAP-ECPs) and EMZ immediate-release pellets (EMZ-IRPs). The reference compound pellets were prepared simulating the drug delivery system of VIMOVO®. In vivo pharmacokinetics, EMZ-MRPs had significantly larger AUC0–t (p < 0.01), 1.67 times more than that of EMZ-IRPs, and prolonged mean residence time (7.55 ± 0.12 h) than that of IRPs (1.46 ± 0.39 h). NAP-CSPs and NAP-ECPs showed similar AUC0–t. Compared to the reference compound pellets, the novel-NAP/EMZ compound pellets did not show distinct differences in histological mucosal morphology. However, biochemical tests exhibited enhanced total antioxidant capacity, increased nitric oxide content and reduced malondialdehyde level for novel-NAP/EMZ compound pellets, indicating that the acid-independent action took effect. The gastric pH values of novel-NAP/EMZ compound pellets were at a low and stable level, which could ensure normal physiological range of human gastric pH. As a result, the novel-NAP/EMZ compound pellets may be a more suitable formulation with potential advantages by improving bioavailability of drug and further reducing undesirable gastrointestinal damages.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Jia Tao,Qiang Zeng,Lishi Wang
Compared to visible light, near infrared (NIR) light is attractive for the development of novel assay method because it can provide deeper imaging penetration and lower fluorescence background and scattering intensity. In this paper, near infrared (NIR) quantum dots (QDs) were synthesized under a convenient and mild condition, afterwards applied to highly sensitive detection of Cu 2+ and monitoring the change of Cu 2+ concentration through in vitro and in vivo fluorescent imaging. The quenching of NIR light was resulted from the aggregation of NIR QDs induced by the competitive binding between 3-mercaptopropionic acid (MPA) and the Cu 2+ present in the solution. A low detection limit of 5 × 10 −8 M and a broad linear detection range from 1 × 10 −7 to 5 × 10 −5 M could be realized in this strategy for the quantitative evaluation of Cu 2+ . This NIR QDs based sensor possess high selectivity, rapid response and excellent photostability. Furthermore, the great potential of this NIR nanosensor has also been fully proved by the in vitro cellular imaging and in vivo imaging.
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APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Fang Wang,Xianhong Zhang,Yuhong Ma,Wantai Yang
The hybrid composite of poly(3,4-ethylenedioxythiophene) (PEDOT) and halloysite nanotubes (HNTs) was synthesized by a two-step process. First, poly(sodium styrene sulfonate) (PSSNa) was grafted onto HNTs via surface initiated atom transfer radical polymerization. Then with the HNTs- g -PSS as a template and the grafted PSS chains as the counterion dopant, PEDOT was precipitated onto the template via in situ oxidization polymerization of EDOT to form HNTs@PEDOT hybrid composites. The conductivity of HNTs@PEDOT can reach up to 9.35 S/cm with the content of 40% HNTs- g -PSS, which increased almost 78 times than that of pure PEDOT (about 0.12 S/cm) prepared at the similar condition. Further treated with p -toluenesulfonic acid (TsOH) as external dopant, the conductivity of HNTs@PEDOT increased to 24.3 S/cm. The electrochemical properties of the composites were investigated with cyclic voltammetry, galvanostatic charge-discharge and electrochemical impedance spectroscopy with three-electrode cell configuration. The results showed that the capacitance of HNTs@PEDOT composite increased 55% than that of pure PEDOT.
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PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2017
·Vol. 34
·期 5
·1600423
·
IF 4.474
Lipei Yue,Weidong Li,Yingjie Cao,Yongping Bai
A core–shell composite has been synthesized through in situ polymerization in emulsion with an average of 205 nm of diameter. Each composite consists of a graphene oxide (GO) core and a poly(methyl methacrylate/butyl acrylate) shell. The latex is homogeneous without any aggregation after stability testing in normal temperature for 100 d and can be applied as an ideal conductive adhesive whose glass transition temperature ( T g ) is under −30 °C and lucid conductive film whose T g is above 17 °C. There exists half core–shell structure in the composite with part of GO exposed which contributes to the electrical conductivity of film formed by composite. The electrical conductivity of the composite is sensitive to humidity, increasing from 0.233 to 0.357 S m −1 , while the related humidity ranges from 0% to 60%. The flexible aliphatic shell established by polyacrylate chains with nanolevel of interspaces makes it easy for hydrone to move in and interact with the oxygen groups on the chains, and then the interaction enhances the difficulty for hydrone to move out, on account of which film formed by core–shell composite can hold hydrone and exhibit advanced electrical conductivity in high humidity atmosphere.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2017
·
IF 3.671
Ping Shao,Qiang Qiu,Jie Xiao,Yueqiong Zhu,Peilong Sun
The aim of the present study was to examine how factors (pH, temperature, iron chelators, and free radical scavengers) influence on the chemical stabilities of β-carotene encapsulated by Ulva fasciata polysaccharide (UFP)-stabilized emulsions and the bioaccessibility under in vitro oral-gastro-intestinal digestion conditions. The concentration of β-carotene in emulsions under different environments was determined. Changes in droplet properties, particle size and microstructure, throughout the oral-gastric-intestinal model were measured and the rate and extent of lipid digestion were recorded in the simulated small intestine fluid. Our results suggested that the pH had a significant effect on the stability of β-carotene, a rapid degradation at acidic environment and a relatively higher stability at high pH (6.0 and 7.0) were observed. β-carotene in emulsions was highly sensitive to extreme temperature (80 °C, 90 °C, and 100 °C). Addition of EDTA or α-tocopherol significantly increased the stability of β-carotene. Meanwhile, digested UFP-stabilized emulsions showed higher lipid digestion rate than GA-stabilized emulsions and BP-stabilized emulsions. UFP was capable of increasing the bioaccessibility of β-carotene in emulsions compared to GA and BP. Therefore, UFP-stabilized emulsion is a promising delivery system to promote applications of β-carotene in functional food and beverage system.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2017
·Vol. 409
·期 8
·2239-2246
·
IF 3.431
Zhang Jie,Zhang Tiehua,Guan Tianzhu,Yu Hansong,Li Tiezhu
Widespread use of bisphenol A (BPA) and other bisphenol analogues has attracted increasing attention for their potential adverse effects. As environmental endocrine-disrupting compounds (EDCs), bisphenols (BPs) may activate a variety of nuclear receptors, including glucocorticoid receptor (GR). In this work, the binding of 11 BPs to GR was investigated by fluorescence polarization (FP) assay in combination with molecular dynamics simulations. The human glucocorticoid receptor was prepared as a soluble recombinant protein. A fluorescein-labeled dexamethasone derivative (Dex-fl) was employed as tracer. Competitive displacement of Dex-fl from GR by BPs showed that the binding affinities of bisphenol analogues were largely dependent on their characteristic functional groups. In order to further understand the relationship between BPs structures and their GR-mediated activities, molecular docking was utilized to explore the binding modes at the atomic level. The results confirmed that structural variations of bisphenol analogues contributed to different interactions of BPs with GR, potentially causing distinct toxic effects. Comparison of the calculated binding energies vs. experimental binding affinities yielded a good correlation ( R
2 = 0.8266), which might be helpful for the design of environmentally benign materials with reduced toxicities. In addition, the established FP assay based on GR exhibited the potential to offer an alternative to traditional methods for the detection of bisphenols.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 30
·8813-8820
·
IF 3.125
Fu Zhaofu,Li Gongke,Hu Yufei
A novel chemiluminescence (CL) system based on the reaction of fluorescent water-soluble nitrogen-rich quantum dots (N-dots) and diperiodatoargentate(III) (DPA) was developed. The prepared N-dots have a small size (≤10 nm) and high percentage of nitrogen (39.9 %), which exceeds the content of carbon in the same N-dots. The N-dots exhibit characteristic blue fluorescence under UV light and up-conversion luminescence. The relatively intense CL emission is based on the direct oxidation of N-dots by DPA. The CL emission may be attributed to the high nitrogen content and the special structure of the N-dots. The CL mechanism of N-dots and DPA was investigated by using CL, UV–Vis absorption, IR, fluorescence, and radical scavenging experiments. This investigation provides a way to study the optical properties of N-dots. The analytical applicability of the N-dots and DPA CL system in the determination of ferulic acid (FA) was explored. The CL intensity was linearly proportional to the concentration of ferulic acid from 3.0 × 10 −7 to 1.0 × 10 −5 g mL −1 with a detection limit of 8.0 × 10 −8 g mL −1 (3σ); the relative standard deviation was 2.4 % for 4.0 × 10 −7 g mL −1 FA ( n = 9). The proposed method was successfully applied to the determination of ferulic acid in Angelica sinensis . The study provides valuable insight into the role of nitrogen-rich quantum dots in CL.
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ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Weiyong Yuan,Xiaoyan Wang,Xiaoling Zhong,Chang Ming Li
The development of efficient and low-cost hydrogen evolution reaction (HER) catalysts is critical for storing energy in hydrogen via water splitting but still presents great challenges. Herein, we report synthesis of three-dimensional (3-D) hierarchical nanoporous carbon (HNC) supported transition metal phosphides (TMPs) for the first time by in situ growth of CoP nanoparticles (NPs) in CaCO3 NP-templated Cinnamomum platyphyllum leaf extract-derived carbon. They were subsequently employed as a HER catalyst, showing an onset potential of 7 mV and an overpotential of 95.8 mV to achieve 10 mA cm–2, a Tafel plot of 33 mV dec–1, and an exchange current density of 0.1182 mA cm–2, of which the onset overpotential and the Tafel plot are the lowest reported for non-noble-metal HER catalysts, and the overpotential to achieve 10 mA cm–2 and the exchange current density also compare favorably to most reported HER catalysts. In addition, this catalyst exhibits excellent durability with negligible loss in current density after 2000 CV cycles ranging from +0.01 to −0.17 V vs RHE at a scan rate of 100 mV s–1 or 22 h of chronoamperometric measurement at an overpotential of 96 mV and a high Faraday efficiency of close to 100%. This work not only creates a novel high-performance non-noble-metal HER electrocatalyst and demonstrates the great advantages of the in situ grown 3-D HNC supported TMP NPs for the electrocatalysis of HER but also offers scientific insight into the mechanism for the in situ growth of TMP and their precursor NPs, in which an ultralow reactant concentration and rich functional groups on the 3-D HNC support play critical roles.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2016
·
IF 2.76
Binbin Shao,Yiyi Guan,Zeyuan Tian,Xiaohong Guan,Deli Wu
Structural Fe(II) Hydroxide (SFH) was used to remove As(III) and As(V) from aerated synthetic wastewater with initially 30 mg/L As at neutral pH values. Batch experiments were performed to investigate the influence of O 2 , pH and coexisting HPO 4 2⿿ on As removal by 0.5 g (Fe)/L SFH. The results suggested that removal efficiency for As(III) and As(V) were ⿼60% and ⿼50% under anoxic conditions at initial pH 7.0 and 9.0, respectively. However, the efficiency increased to over 90% for both As(III) and As(V) under oxic conditions. The in situ mineral transformation of SFH to newly formed Fe(II/III) and FeOOH was responsible for the robust removal. As(III) removal was nearly unaffected by HPO 4 2⿿ (1.0 mM), while obviously inhibition was observed in the case of As(V). The microstructure of fresh and reacted SFH was characterized by FTIR and XPS and these results indicate that As was removed via inner-sphere complexation and precipitation. XPS studies showed that As(III) was converted to As(V) after 2 h of reaction in the pH range of 7.0⿿9.0, which is attributable to enhanced formation of Fe(IV) by the Fenton reaction during mineral transformation. In practice, results of this study suggest that aeration is advantageous to significantly enhancement of As(III) oxidation and removal with highly reactive SFH.
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