Biomaterials Advances
·
2026
·
IF 6.7
Jieyu Su,Yuxin Zhang,Yongqiang Cao,Tiantian Ren,Kunxi Zhang,Dong Chen,Jingbo Yin
PBLG HNMs feature open-hollow nanofibrous architecture and injectability. • PBLG self-assembly drives the internal ECM-like nanofibrous network. • Covalent GHK-Cu grafting affords stable osteogenic-angiogenic activity. • PBLG-GCu HNMs promote BMSC osteogenesis and HUVEC angiogenesis. Biomimetic fibrous microspheres with a high specific surface area hold substantial promise for bone tissue engineering. In this study, asymmetric open-hollow nanofibrous microspheres (HNMs) were fabricated from polypeptide poly( γ -benzyl- L -glutamate) (PBLG) via a combination of emulsion and thermally induced phase separation, yielding PBLG HNMs. To further impart biofunctionality, the copper peptide (GHK-Cu) was covalently grafted onto the microspheres to obtain osteoinductive and pro-angiogenic PBLG-GCu HNMs. The optimized microspheres exhibited an average diameter of 372 ± 102 μm, which is suitable for injectability, and an opening size of 219 ± 53 μm, enabling efficient cellular infiltration. The internal surface featured an interconnected nanofibrous network with a fiber diameter of 417 ± 78 nm, mimicking the extracellular matrix (ECM) microenvironment and providing abundant cell-interactive sites. Live/Dead staining and CCK-8 assays confirmed the cytocompatibility of the PBLG-GCu HNMs. Compared with non-functionalized PBLG HNMs, PBLG-GCu HNMs enhanced bone marrow mesenchymal stem cell (BMSC) mineralization and upregulated osteogenic gene expression, with Runx2, OPN, and OCN expression increased by 1.61-, 3.53-, and 2.29-fold, respectively. In addition, the tube formation assay verified robust angiogenic stimulation. Overall, the PBLG-GCu HNMs integrated hierarchical structural biomimicry with dual osteogenic-angiogenic bioactivity, exhibiting great potential as injectable scaffolds for repairing irregular bone defects.
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PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2026
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IF 6.2
Chengbo Zhou,Xin Zhong,Sen Wang,Wenwu Zou,Ji Sun,Chenwei Lin,Qichang Yang,Shuhao Li
Common UV filters (OBZ, AVB, OCR, OMC) widely exist in the environment and organisms, raising global concerns about their ecological and physiological risks. However, the molecular response mechanisms of higher plants to these emerging contaminants remain largely unexplored. To address this research gap, we herein employed quantitative proteomics to examine root-specific responses in pakchoi ( Brassica rapa ssp. chinensis ) following exposure to each of the four UV filters. Across all treatments, a total of 8656 proteins were quantified, with 632, 482, 251, and 393 differentially expressed proteins (DEPs) identified under OBZ, AVB, OCR, and OMC exposure, respectively. These DEPs were primarily enriched in key metabolic pathways, including reactive oxygen species (ROS) scavenging, glycolysis (EMP)–tricarboxylic acid (TCA) cycle, respiratory electron transport chain, fatty acid metabolism, and amino acid biosynthesis. All four UV filters induced pronounced oxidative stress in pakchoi roots, as indicated by elevated ROS levels and increased lipid peroxidation. Proteins involved in EMP (6-phosphofructokinase, pyruvate kinase) and the TCA cycle (isocitrate dehydrogenase, malate dehydrogenase) exhibited significant downregulation. In contrast, proteins associated with fatty acid metabolism (aldehyde dehydrogenase, long-chain fatty acid omega-monooxygenase, acyl-CoA oxidase and Delta3-Delta2-enoyl-CoA isomerase) were predominantly upregulated. These proteomic patterns suggest a potential alteration in EMP–TCA cycle, and lipid metabolic processes. Concurrently, proteins associated with antioxidant enzymes (glutathione S-transferases, peroxidases and catalases) were predominantly upregulated. These proteomic findings are consistent with the increased peroxidases and catalases enzyme activity observed at the physiological level. Collectively, these findings provide a comprehensive proteomic overview of pakchoi root responses to UV filter exposure and offer novel insights into the underlying mechanisms of phytotoxicity, and provide a reference for the breeding of crop resistant varieties to ensure agricultural productivity and food safety.
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Food Bioscience
·
2026
·
IF 6.2
Xiangkun Du,Yasen Wang,Buqing Wang,Yu Shen,Shu Teng,Jiawen Bai,Zijie Li
Saccharomyces cerevisiae spores have emerged as a novel surface display host and have been successfully applied in the biosynthesis of useful products. To construct a dual-enzyme co-display and assembly system on the surface of S. cerevisiae spores, the fluorescent proteins were initially used as the model proteins to prove the feasibility. The result of the in vitro assembly experiment of L-arabinose isomerase from Bacillus coagulans ( Bc AI) and mannose-6-phosphate isomerase from Geobacillus thermodenitrificans ( Gt MPI) confirmed that the assembled complex could significantly improve the biosynthetic efficiency of the rare sugar L-ribose. Subsequently, Bc AI and Gt MPI were co-displayed on the spore surface and further assembled via the SpyTag/SpyCatcher system to realize the biosynthesis of L-ribose. The maximum conversion rate of L-ribose reached approximately 16% with 10 g/L L-arabinose as the substrate. Nevertheless, the achieved L-ribose conversion rate of 16% is moderate compared with previously reported values. Even with this limitation, this study establishes a feasible spore-surface assembly strategy for multi-enzyme cascades, laying a technical foundation for the biosynthesis of high-value products.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·Vol. 74
·期 30
·23947-23964
·
IF 6.7
Zhou Xinyue,Yu Wenxin,Cui Yanhong,Meng Xiang,Zhang Jian,Jiang Qingxue,Yang Bo,Ma Xiaoran,Ma Lin,Liang Qingwei,Wang Xuemin
Salt stress disrupts photosynthesis, ion homeostasis,
and antioxidant
systems, thereby inhibiting plant growth and reducing the yield and
quality. This study investigated the physiological and molecular mechanisms
of salt stress alleviation by foliar application of different concentrations
of silicon dioxide nanoparticles (SiO 2 NPs) in oats under
150 mM NaCl. Physiological and transcriptomic analyses were performed.
300 ppm of SiO 2 NPs significantly promoted growth and photosynthesis,
enhanced antioxidant enzyme activities, reduced oxidative damage,
and maintained Na + /K + balance while improving
nutritional quality. SiO 2 NPs regulated genes involved
in photosynthesis and carbon metabolism and upregulated the ion transporter
genes HKT1;1 , HKT7 , and HKT9 and ROS signaling genes MPK6 and WRKY33 . HKT proteins were localized to the plasma membrane.
Weighted correlation network analysis (WGCNA) identified six hub genes
associated with the growth and photosynthetic traits. Overall, SiO 2 NPs enhance salt tolerance, yield, and quality in oat by
maintaining Na + /K + homeostasis, activating ROS
signaling, and sustaining photosynthesis and carbon metabolism.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·Vol. 74
·期 29
·22965-22974
·
IF 6.7
Li Mengyu,Fu Chenchen,Zhuang Wei,Zhang Keke,Xu Jingliang,Wang Zhenfu
D-Amino acid oxidase (DAAO) catalyzes the oxidation of
D-phosphinothricin
(D-PPT) to produce optically pure L-PPT, but low catalytic efficiency
limits its industrial application. Here, a synergistic strategy combining
geometric remodeling and charge engineering was applied to enhance
the oxidation activity of our previously developed stable variant
TIF-DAAO (S18T/V7I/Y132F) toward D-PPT. Systematic analysis of the
active pocket-guided alanine and neutral hydrophilic (Ser/Thr/Tyr)
scanning mutagenesis revealed position-specific steric constraints.
Subsequent Arg/His scanning tailored the positively charged microenvironment
around D-PPT, leading to a triple mutant ATR (F58A/Q335T/P221R) with
improved electrostatic complementarity and spatial fit. ATR showed
a 10.7-fold increase in catalytic activity, a 35-fold improvement
in substrate affinity, and a 380-fold higher catalytic efficiency
( k cat / K M )
toward D-PPT. Molecular dynamics simulations provided mechanistic
insight. Our results demonstrate that the synergistic optimization
of substrate tunnel geometry and the electrostatic microenvironment
effectively boosts DAAO activity, offering a rational strategy for
engineering industrial biocatalysts.
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POLYMER COMPOSITES
·
2024
·
IF 4.8
Feng Yang,Linfeng Fan,Xiang Qiu,Kaiwen Shi,Hao Pan,Cijun Shuai,Pei Feng
Nano-hydroxyapatite (nano-HAP)/poly (l-lactic acid) (PLLA) bone scaffold is expected to overcome the deficiencies and achieve the complementary advantages of individual constituents, but the weak interfacial bonding due to their thermodynamic incompatibility is detrimental to the mechanical properties. Herein, the PLLA chains were grafted onto nano-HAP with 3-aminopropyltriethoxysilane (KH550) as a coupling reagent to enhance the interfacial bonding with PLLA. Specifically, the silicon hydroxyl group produced by KH550 hydrolysis could form covalent bonding with the hydroxyl group of nano-HAP, and the amino group of KH550 initiated the ring-opening polymerization of l-lactide monomers to graft PLLA chains onto nano-HAP more effectively, leading to a higher grafting ratio of 16.7% compared with 7.2% in direct grafting without KH550 modification. Consequently, the tensile and compressive strength of the modified nano-HAP/PLLA scaffold were improved by 40.8% and 59.5% enhancement due to the enhanced interfacial bonding in the composite scaffold, respectively, compared to the original nano-HAP/PLLA scaffold. Additionally, the bone scaffold was conducive to cell adhesion and proliferation, making it an ideal candidate for bone defect repair. Highlights Amino-functionalized nano-HAP boosted the grafting efficiency of PLLA chains. Interfacial bonding between nano-HAP and matrix was enhanced. Bone scaffold showed better mechanical properties and benign cytocompatibility.
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MOLECULES
·
2025
·Vol. 30
·期 12
·2526
·
IF 4.6
Yingya Qiu,Jiangxuan Lai,Yuhan Zhang,Sheng Fang,Zili Guo,Xianrui Liang
Despite their broad pharmacological potential, the clinical application of stilbenes derived fromCajanus cajan(L.) Millsp. leaves (CCMLs) is limited by their poor aqueous solubility, resulting in low oral bioavailability. In this study, an inclusion complex of stilbenes extracted from CCMLs and 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) was successfully developed to improve their solubility and oral bioavailability. The preparation conditions were optimized using the response surface methodology, with an optimum mass ratio of CCML extract (CCMLE) to HP-β-CD of 1.0:8.2 (g/g) and an optimal temperature of 33 °C for 2 h. The maximum inclusion efficiency of stilbenes was 82%, and the physicochemical properties of the inclusion complex were characterized. Both in vitro dissolution studies and in vivo pharmacokinetic evaluation demonstrated that HP-β-CD encapsulation significantly improved the solubility and dissolution rate of stilbenes compared to those of unformulated CCMLE. Notably, the relative bioavailability of cajaninstilbene acid (CSA), longistyle C (LLC) and 4-O-methylpinosylvic acid (MPA) increased to 198%, 177% and 131%, respectively. This work provides a simple and effective strategy to enhance the solubility and bioavailability of naturally derived stilbenes, offering promising potential for the development of other insoluble natural components for both functional food and pharmaceuticals.
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Food Frontiers
·
2025
·
IF 6.9
Qiuming Liu,Qingyu Ma,Jiao Li,Liping Sun,Yongliang Zhuang,Yuanlong Tang
This study aimed to develop a green and efficient technique for extracting rambutan peel polyphenols, while systematically evaluating chemical components, the water solubility, storage stability, in vitro antioxidant activity, and digestive enzyme inhibitory potential of the extracts. Subcritical water (SW) alone and SW followed by enzymatic hydrolysis (SW-EH) were employed to extract rambutan peel polyphenols. The optimal conditions of SW extraction were temperature of 130°C, time of 30 min, and liquid–solid ratio of 80:1 mL/g, which yielded a total phenol content (TPC) of 226.54 mg gallic acid equivalents per gram of dry weight powder. A total of 39 phenolics were identified with ellagic acid as the predominant compound. SW-EH extract maintained similar phenolic profiles but contained less soluble dietary fiber compared to SW extract. The transmittance and polyphenol solubility of SW-EH extract were higher than those of SW extract in the concentration range of 5 to 100 mg/mL. The ellagic acid content in the SW and SW-EH extracts decreased by 19.05% and 1.71%, respectively, after 40 days of storage. Both extracts demonstrated comparable antioxidant activities superior to ascorbic acid, while EH treatment greatly enhanced α-glucosidase inhibitory activity compared to SW extract. With superior water solubility, storage stability, antioxidant activity, and digestive enzyme inhibitory potential, SW-EH extract is a promising product for a wide range of applications.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yingqi Liang,Yupeng Liu,Jun Wu,Lingyun Li,Xue Wu,Zekun Yan,Mingyu Chen,Elena V. Ushakova,Caishi Huang,Songnan Qu
The development of stimuli-responsive fluorescent nanomaterials with molecular pores is crucial for real-time monitoring of the delivery of functional non-fluorescent small molecules. Herein, a new type of yellow emissive porous carbon dots (CDs) derived from 3,9-perylenedicarboxylic acid was synthesized, employing a radical-assisted method under ambient pressure and mild heating conditions, and it is the smallest porous nanoplatform based on CDs. The molecular pores in the CDs can absorb L-arginine via intermolecular hydrogen bonds to form CDs and L-arginine complex (Arg@CDs). The absorbed L-arginine molecules in the molecular pores break the interlayer π – π interactions in the core due to the steric hindrance, leading to a fluorescence color shift from yellow to cyan. When exposed to an acidic environment, the absorbed L-arginine can be released, resulting in a recovery of yellow fluorescence. In vitro, Arg@CDs can be endocytosed by cells and localized to lysosomes, in which the acidic environment causes L-arginine release from the Arg@CDs with a fluorescence color change from cyan to yellow. Thus, Arg@CDs can be used as novel fluorescence color-sensitive pH probe for intracellular dynamic fluorescent imaging. Furthermore, in the mouse scald wound model, intravenous injection of Arg@CDs can significantly accelerate wound healing by promoting macrophage proliferation and anti-inflammatory action.
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ULTRASONICS SONOCHEMISTRY
·
2025
·
IF 9.7
Hong-Fu Zhao,Si-Yi Han,Lu Xiao,Bo-Kang Yu,Ning Xia,Ying-Hua Zhang
Corn silk ( Zea mays L. ), an accessory product of maize, is an unconventional component with potential bioactive compounds. In this study, isoorientin was extracted from maize whiskers by ultrasound-assisted extraction, and the response surface optimization resulted in an extraction amount of 1.26 mg/g. A quantitative structure-effect relationship (QSAR) prediction model was developed, and the R 2 and RMSE of the XGBoost regression algorithm were 0.9582 and 0.01823, respectively. Interpretative analyses of the prediction model were carried out by using the SHAP visualization, which showed that the charge, structure, and lipid solubility of the molecules play a key role. Several anti-inflammatory compounds were screened by combining molecular docking and molecular dynamics. After oral toxicity testing, it was determined that isoorientin performed the best and had a semi-inhibitory concentration of 18.38 μg/mL. This study improves the high-value utilization of maize whiskers and provides a theoretical basis for the rapid screening of food-borne anti-inflammatory compounds.
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JOURNAL OF ASTHMA
·
2026
·
IF 1.3
Xueting Yang,Yu Guo,Miao Bao,Xin Lu,Qi Liu,Yumei Wang,Jie Zhang,Honglian Zhang,Huimin Sui,Hao Zhang,Huiyu Wang
Objective Allergic asthma (AAS) affects over 300 million people globally, creating significant health and economic challenges. Belamcanda chinensis (L.) DC, known as SG, is traditionally used in the “Shegan-Mahuang decoction” for AAS treatment, yet its separate therapeutic effects are underexplored. This study aims to investigate the anti-AAS properties of isoflavones derived from SG.Methods Using a mouse model induced by house dust mite (HDM), the research involved treating HDM-sensitized mice with tectorigenin, dexamethasone, and varying doses of SG (low, medium, and high). Anti-inflammatory effectiveness was assessed through assays measuring cytokines, along with histological techniques such as hematoxylin-eosin and periodic acid-Schiff staining, alongside leukocyte counts. Additionally, gas chromatography-mass spectrometry was utilized to analyze tissue metabolic changes.Results The medium dose of SG (435 mg/kg) significantly diminished inflammatory cell infiltration, goblet cell hyperplasia, and mucus production. Furthermore, SG treatment reduced cytokine levels and inflammatory cells in bronchoalveolar lavage fluid and lowered serum HDM-IgE and total IgE levels, showcasing a notable reduction in inflammation. The metabolic profile of lung tissue from SG-treated mice closely resembled that of dexamethasone-treated mice, with levels of seven amino acid-related metabolites normalizing, aligning with decreased inflammatory responses.Conclusions This study highlights the potential of SG isoflavone extract as an effective therapeutic strategy for AAS, shedding light on the mechanisms through which SG may alleviate allergic asthma symptoms.
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POLYMER
·
2021
·
IF 4.43
Hongyu Zhu,Yu Liu,Dongxu Gu,Zikun Rao,Yang Li,Jianyuan Hao
It is a challenging work to design novel responsive materials by controlling the secondary structure of polypeptide , especially β-sheet. We designed and synthesized a novel dual responsive diblock copolymer of methyl poly (ethylene glycol)- b -poly (O-benzyl- l -threonine) (mPEG- b -PBnLT). The copolymer solutions exhibited unusual and reversible gel-to-sol-to-gel transition behavior with temperature, and the transition temperature window could be easily adjusted by changing copolymer concentration or PBnLT block length . It was confirmed that low temperature gel-to-sol transition was due to the disassembly of the preliminary β-sheet layered nano-assemblies that induced the transformation of self-organized morphology from nanosized fibrils to spherical aggregates, while the high temperature sol-to-gel transition was ascribed to the dehydration of PEG segments. The disassembly of β-sheet structures was caused by the change in the polarity of the pendant benzyl ether bond of PBnLT block in water with temperature. This stimuli-responsiveness of secondary self-assembled nanostructures and the corresponding change in macroscopic phase transition inspires a brand-new strategy for structural design and functional control of polypeptide materials.
研究领域:
高分子科学
·
材料化学
·
纳米技术
·
智能材料
·
生物聚合物
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危险品化学品经营许可证（带存储）